CN115633686A - Active component of larch fruit cabbage moth attractant, attractant and attracting method - Google Patents

Active component of larch fruit cabbage moth attractant, attractant and attracting method Download PDF

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CN115633686A
CN115633686A CN202211260175.7A CN202211260175A CN115633686A CN 115633686 A CN115633686 A CN 115633686A CN 202211260175 A CN202211260175 A CN 202211260175A CN 115633686 A CN115633686 A CN 115633686A
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cis
dodecene acetate
attractant
larch
dodecene
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CN115633686B (en
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刘红霞
郝玉杰
荆小院
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Inner Mongolia Nongxin Technology Co ltd
Shanxi Yuanjie Technology Co ltd
Chongzhutou Suzhou Biotechnology Co ltd
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Inner Mongolia Nongxin Technology Co ltd
Shanxi Yuanjie Technology Co ltd
Chongzhutou Suzhou Biotechnology Co ltd
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    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract

The invention provides an attractant active component of a larch fruit cabbage moth attractant, which comprises the following components in parts by mass: 1 trans-9-dodecene acetate, cis-9-dodecene acetate. The invention also provides an attractant for the larch fruit moth, which comprises an active component and a nonpolar organic solvent, wherein the active component comprises the following components in parts by mass: 1 trans-9-dodecene acetate, cis-9-dodecene acetate. The invention also provides a method for attracting the larch fruit cabbage moth. The larch fruit cabbage moth attractant provided by the invention has the advantages of convenient use, high efficiency, no toxicity, no pollution, no harm to natural enemies, low cost and long validity period.

Description

Active component of larch fruit cabbage moth attractant, attractant and attracting method
Technical Field
The invention belongs to the field of pest control, and particularly relates to an active component of a larch fruit moth attractant, the attractant and an attracting method.
Background
Larch fruit cabbage moth (Retmia perangustana Snellen), with pupa length of 4.5 to 6.5mm, yellow brown; white cocoon and oblong shape; the larvae grow 8-10mm in length, are yellow white, are yellow brown in head, are yellow brown in the front chest and back board and dark brown in the rear part, and have a toe hook single sequence; the egg length is 0.6 to 0.7mm, the width is about 0.4mm, the egg is white or slightly reddish, peach-red when in use, flat and oval, and one end is pointed. The length of the imago is 3.2 to 5.2mm, the imago is brown, and the wingspan is 10 to 15mm. Domestic distribution is in Heilongjiang, jilin, liaoning and inner Mongolia; abroad in eastern siberia, coastal areas and parts of europe.
1 year or 2 years and 1 generation of larch fruit cabbage moth, overwintering the trunk with pupa (few larva) cocoons under the skin of the trunk, enabling adults to begin to eclosion and spawn in the middle 5 th month ten days, and taking the last 5 th month ten days as a full period; eggs begin to hatch in the middle 6 th of the month, and the middle and last 6 th of the month are the full period. Larvae leave the cones to pupate in the middle 7 th to the upper 8 th months, which is the top stage in late 7 months. The larvae are 4 years old, the larval stage is about 36 days, and the damage stage is 40-45 days. The eclosion is carried out in the daytime, and the pupa shell is hung outside the cocoon shell after the eclosion. The imagoes lay eggs on the bract scales at the base parts of the cones singly, each cone usually has a plurality of eggs, 2-3 eggs are rare, and the egg period is about 10 days. After the larvae are hatched, the larvae are drilled into scales to eat; after molting for 1 time, transferring and feeding endosperm of immature seeds and nearby scales by larvae, and opening a wormhole outlet by silking to move around a fruit shaft so that the cones are filled with insect feces; 1 larva can harm 70% of fruit scale of 1 cone, and when the larva occurs greatly, each cone has 2-15 larvae, the number of the larvae is the largest in the sunny slope, the second in the mountain top and the smallest in the cloudy slope. The natural enemies comprise the fringed coilia, the long-body cocoons, the pine cocoons, the angry cocoons and the like, and the parasitic rate is 20% -45%; the pupal stage fungal parasitism rate reaches 40 percent, and the larch head-bud insect-killing agent is one of important pests of larch cones. Its larva eats the developing cone, and eats the damaged scale and seeds along the axis of the cone, and the damaged scale becomes withered yellow, the cone bends and deforms, and the undamaged seeds in the cone develop badly, thus seriously affecting the yield and quality of the seeds.
The control measures of the larch leaf roller moth mainly comprise forestry measures, biological control, physical control and chemical control. The forestry measures mainly comprise breeding insect-resistant tree species and building mixed forests; biological control comprises protection, artificial propagation, utilization of natural enemies and the like; the physical control mainly utilizes phototaxis of the imagoes to trap and kill the imagoes in the eclosion period; chemical control is mainly carried out by using pesticides. Insect sex pheromone is a green prevention and control method for diseases and insect pests, has the advantages of high sensitivity, strong selectivity, no harm to natural enemies, environmental protection and the like, and is widely used at home and abroad as a direct or indirect pest control means. At present, more than 1000 kinds of insect sex pheromones or analogues are identified all over the world, tens of millions of hectares of agricultural crops and forest areas use sex pheromone products for pest control, and more than 100 kinds of sex pheromone products are put into use in China.
At present, relevant documents or reports about the pheromone of the larch cone moth are not found.
Disclosure of Invention
An object of the present invention is to provide an attractant active component, attractant and method for larch fruit moth and to provide at least the advantages which will be described later.
The invention also aims to provide the larch fruit cabbage moth attractant active component, the larch fruit cabbage moth attractant and the attracting method, which have the advantages of convenient use, high efficiency, no toxicity, no pollution, no harm to natural enemies, low cost and long validity period.
The technical scheme of the invention is as follows:
the attractant active component of the larch fruit cabbage moth comprises the following components in parts by mass: 1 trans-9-dodecene acetate, cis-9-dodecene acetate.
Preferably, the larch fruit moth attractant active component also comprises cis-8-dodecene acetate.
Preferably, the active components of the larch fruit moth attractant comprise, by mass, 1:1:1 trans-9-dodecene acetate, cis-9-dodecene acetate and cis-8-dodecene acetate.
Preferably, the larch fruit moth attractant active component also comprises cis-10-dodecene acetate.
Preferably, the active components of the larch fruit moth attractant comprise, by mass, 1-2:1:1:1 trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate.
The larch fruit cabbage moth attractant comprises an active component and a nonpolar organic solvent, wherein the active component comprises the following components in parts by mass: 1 trans-9-dodecene acetate, cis-9-dodecene acetate.
Preferably, in the larch fruit moth attractant, the active components further comprise cis-8-dodecene acetate, trans-9-dodecene acetate, cis-9-dodecene acetate and cis-8-dodecene acetate, and the mass part ratio of the cis-8-dodecene acetate to the cis-8-dodecene acetate is 1:1:1.
preferably, in the larch fruit moth attractant, the active components further comprise cis-10-dodecene acetate, trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate, and the mass part ratio of the cis-10-dodecene acetate to the trans-9-dodecene acetate is 1-2:1:1:1.
the method for attracting the larch fruit cabbage moth comprises the following steps:
selecting one of trans-9-dodecene acetate and cis-9-dodecene acetate, or trans-9-dodecene acetate, cis-9-dodecene acetate and cis-8-dodecene acetate, or three combinations of trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate, and mixing nonpolar organic solvents to prepare the attractant; then dripping the natural rubber plug into a small reverse opening of the natural rubber plug, and placing the natural rubber plug above a sticky plate in a triangular trap to prepare the trap; the trappers are hung on the branches of the pine trees and hung at an upper wind port, the distance between the trappers is 0.5 to 1.2 meters, and the distance between the trappers is about 50 meters.
The application prospect and the effect analysis of the invention are as follows:
1. monitoring insect pest situation: the insect condition forecast method can timely and accurately know the occurrence and growth conditions of the larch fruit cabbage moth population in the fruit tree forest, and provides scientific basis for timely and effective prevention and control. The attractant of the invention can be used for detecting and predicting the insect situation, has high sensitivity, strong specificity, convenient use and low cost, and is suitable for general popularization.
2. Mass trapping: according to the insect situation monitoring result, an adult generation curve is drawn, and in the adult generation period, the larch fruit moth attractant provided by the invention is hung in a damaged fruit tree forest, so that male and female adults of the larch fruit moth can be trapped, the population density can be reduced, and the harm degree can be reduced.
The invention is prepared by repeated tests in laboratories and forests, is favorable for protecting natural enemies, avoids using chemical pesticides, is environment-friendly and safe, is convenient to use, has low cost and long validity period, can achieve the aim of effectively preventing and controlling the larch fruit cabbage moth, and has obvious ecological benefit and economic benefit no matter the larch condition is used for forecasting or trapping a large amount of larch.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
The invention provides an attractant active component of a larch fruit moth attractant, which comprises the following components in parts by mass: 1 trans-9-dodecene acetate, cis-9-dodecene acetate.
In one embodiment of the larch fruit moth attractant active component provided by the invention, cis-8-dodecene acetate is further included.
In one embodiment of the active component of the attractant of the larch fruit moth, the active component comprises the following components in parts by mass: 1:1 trans-9-dodecene acetate, cis-9-dodecene acetate and cis-8-dodecene acetate.
In one embodiment of the larch fruit moth attractant active component provided by the invention, cis-10-dodecene acetate is further included.
In one embodiment of the attractant active component of the larch fruit moth provided by the invention, the attractant active component comprises the following components in parts by mass of 1-2:1:1:1 trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate.
The invention also provides an attractant for the larch fruit moth, which comprises an active component and a nonpolar organic solvent, wherein the active component comprises the following components in parts by mass: 1 trans-9-dodecene acetate, cis-9-dodecene acetate.
In an embodiment of the larch fruit moth attractant provided by the invention, the active component further comprises cis-8-dodecene acetate, and the mass part ratio of the trans-9-dodecene acetate, the cis-9-dodecene acetate and the cis-8-dodecene acetate is 1:1:1.
in one embodiment of the larch fruit moth attractant, the active component further comprises cis-10-dodecene acetate, trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate, and the mass part ratio of the cis-9-dodecene acetate to the cis-10-dodecene acetate is 1-2:1:1:1.
the invention also provides a method for attracting the larch fruit moth, which comprises the following steps:
selecting one of trans-9-dodecene acetate and cis-9-dodecene acetate, or trans-9-dodecene acetate, cis-9-dodecene acetate and cis-8-dodecene acetate, or three combinations of trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate, and mixing nonpolar organic solvents to prepare the attractant; then dripping the natural rubber plug into a small reverse opening of the natural rubber plug, and placing the natural rubber plug above a sticky plate in a triangular trap to prepare the trap; the trappers are hung on the branches of the pine trees and hung at an upper wind port, the distance between the trappers and the ground is 0.5-1.2 meters, and the distance between the trappers is about 50 meters.
The applicant collected damaged pine cones in 2022 in 6 months and found that the fruit decay rate of the cones was above 90%. In view of the characteristics of wide range of pinus tabulaeformis insect pests and rapid occurrence, if the pest mechanism mainly harmful to the species of larch fruit cabbage moth can be analyzed and researched, and the indoor and outdoor biological activity measurement is carried out on the pest mechanism, not only can theoretical basis and scientific basis be provided for green prevention and control of pinus tabulaeformis forest, but also the important significance of improving economic benefit and protecting ecological environment is achieved.
The experimental site: quaggers schur bayama, inner mongol, redwood 39.872673n,111.087528e;
the inducing method comprises the following steps: preparing single-component and multi-component larch fruit moth attractant, proportionally mixing the active components with hydrocarbon organic solvent petroleum ether 50 μ L (60-90 deg.C), dropping into the small reverse mouth of natural rubber plug to obtain attracting core, and placing on the upper surface of the adhesive plate in triangular trap. The trapper filled with the Plutella xylostella sex attractant of the invention is hung on the branches of the pine trees, and hung on the upper wind port, 0.5 to 1.2 meters away from the ground, the distance between the trappers is about 50 meters, 5 times of repetition are set for each group of experiments, and the comparison is a blank trapping core added with only 50 mu L of petroleum ether. Trap number was recorded daily and sticky plates were replaced.
Example 1:
a: trans-9-dodecene acetate 200. Mu.g
Cis-9-dodecene acetate 200. Mu.g
Trans-9-dodecenol 200. Mu.g
Cis-9-dodecenol 200 ug
B: trans-8-dodecene acetate 200. Mu.g
Cis-8-dodecene acetate 200. Mu.g
Trans-8-dodecenol 200 ug
Cis-8-dodecenol 200 ug
C: cis-10-dodecenol 200 ug
Cis-10-dodecene acetate 200. Mu.g
Trans-10-dodecenol 200. Mu.g
Trans-10-dodecene acetate 200. Mu.g
D: cis-8-trans-10-dodecenol 200. Mu.g
Cis-8-trans-10-dodecene acetate 200. Mu.g
Trans-8-cis-10-dodecenol 200 ug
Trans-8-cis-10-dodecene acetate 200. Mu.g.
Figure 29537DEST_PATH_IMAGE002
As can be seen from example 1, the trapping efficiency of the mixed type attractant containing three components A, B and C was relatively high.
Example 2:
a1: trans-9-dodecene acetate 200. Mu.g
A2: cis-9-dodecene acetate 200. Mu.g
A3: trans-9-dodecenol 200. Mu.g
A4: cis-9-dodecenol 200 ug
B1: trans-8-dodecene acetate 200. Mu.g
B2: cis-8-dodecene acetate 200. Mu.g
B3: trans-8-dodecenol 200 ug
B4: cis-8-dodecenol 200 ug
C1: cis-10-dodecenol 200 ug
C2: cis-10-dodecene acetate 200. Mu.g
C3: trans-10-dodecenol 200 ug
C4: trans-10-dodecene acetate 200. Mu.g
Figure 13588DEST_PATH_IMAGE004
As can be seen from Table 2, the trapping efficiency of A1, A2, B2 and C2 was high in the monocomponent.
Figure 855642DEST_PATH_IMAGE006
As can be seen from table 3, it contains four components A1, A2, B2, C2, and when A1: a2: b2: c2 is 400. Mu.g: 200 μ g:200 μ g: the trapping efficiency of the mixed type sex attractant was the highest at 200. Mu.g.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the details shown in the description and the examples, which are set forth, but are fully applicable to various fields of endeavor as are suited to the particular use contemplated, and further modifications will readily occur to those skilled in the art, since the invention is not limited to the details shown and described without departing from the general concept as defined by the appended claims and their equivalents.

Claims (9)

1. The attractant active component of the larch fruit cabbage moth attractant is characterized by comprising the following components in parts by mass: 1 trans-9-dodecene acetate, cis-9-dodecene acetate.
2. The larch fruit moth attractant active component of claim 1, further comprising cis-8-dodecene acetate.
3. The larch fruit moth attractant active component of claim 2, comprising, in parts by mass, 1:1:1 trans-9-dodecene acetate, cis-9-dodecene acetate and cis-8-dodecene acetate.
4. The larch fruit moth attractant active component of claim 2, further comprising cis-10-dodecene acetate.
5. The larch fruit moth attractant active component as claimed in claim 4, which comprises the following components in parts by mass of 1-2:1:1:1 trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate.
6. The attractant for the larch fruit cabbage moth is characterized by comprising an active component and a nonpolar organic solvent, wherein the active component comprises the following components in parts by mass: 1 trans-9-dodecene acetate, cis-9-dodecene acetate.
7. An attractant for a larch fruit moth according to claim 6, wherein the active components further include cis-8-dodecene acetate, trans-9-dodecene acetate, cis-9-dodecene acetate and cis-8-dodecene acetate in a mass part ratio of 1:1:1.
8. an attractant for a fruit moth of larch fruit according to claim 6, wherein the active components further comprise cis-10-dodecene acetate, trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate in a mass part ratio of 1 to 2:1:1:1.
9. the method for attracting the larch fruit cabbage moth is characterized by comprising the following steps:
selecting one of trans-9-dodecene acetate and cis-9-dodecene acetate, or one of the three combinations of trans-9-dodecene acetate, cis-9-dodecene acetate and cis-8-dodecene acetate, or trans-9-dodecene acetate, cis-8-dodecene acetate and cis-10-dodecene acetate, and mixing the three combinations with a nonpolar organic solvent to prepare an attractant; then dripping the natural rubber plug into a small reverse opening of the natural rubber plug, and placing the natural rubber plug above an adhesive plate in a triangular trap to prepare the trap; the trappers are hung on the branches of the pine trees and hung at an upper wind port, the distance between the trappers and the ground is 0.5-1.2 meters, and the distance between the trappers is about 50 meters.
CN202211260175.7A 2022-10-14 2022-10-14 Active component of attractant for fall She Songshi plutella xylostella, attractant and attracting method Active CN115633686B (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1429484A (en) * 2001-12-30 2003-07-16 中国科学院动物研究所 European pine shoot moth sex attractant
CN111713496A (en) * 2019-03-22 2020-09-29 广西壮族自治区林业科学研究院 Efficient sex attractant for litchi special-shaped cabbage moths

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1429484A (en) * 2001-12-30 2003-07-16 中国科学院动物研究所 European pine shoot moth sex attractant
CN111713496A (en) * 2019-03-22 2020-09-29 广西壮族自治区林业科学研究院 Efficient sex attractant for litchi special-shaped cabbage moths

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王刚;马惠芬;胡光辉;冯志伟;孟梦;闫争亮;: "实小卷蛾性信息素的研究进展", 西部林业科学, vol. 37, no. 04, 15 December 2008 (2008-12-15), pages 102 - 105 *

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