CN115624652A - Preparation method of polyvinyl alcohol artificial eye tissue engineering substitute material and artificial eye tissue engineering substitute material prepared by same - Google Patents
Preparation method of polyvinyl alcohol artificial eye tissue engineering substitute material and artificial eye tissue engineering substitute material prepared by same Download PDFInfo
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- CN115624652A CN115624652A CN202211324482.7A CN202211324482A CN115624652A CN 115624652 A CN115624652 A CN 115624652A CN 202211324482 A CN202211324482 A CN 202211324482A CN 115624652 A CN115624652 A CN 115624652A
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- polyvinyl alcohol
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- eye tissue
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- 239000004372 Polyvinyl alcohol Substances 0.000 title claims abstract description 131
- 229920002451 polyvinyl alcohol Polymers 0.000 title claims abstract description 131
- 239000000463 material Substances 0.000 title claims abstract description 118
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 239000000017 hydrogel Substances 0.000 claims abstract description 60
- 238000007731 hot pressing Methods 0.000 claims abstract description 34
- 238000011282 treatment Methods 0.000 claims abstract description 30
- 238000002791 soaking Methods 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 24
- 239000004480 active ingredient Substances 0.000 claims abstract description 22
- 230000001939 inductive effect Effects 0.000 claims abstract description 19
- 239000002904 solvent Substances 0.000 claims abstract description 19
- 238000001953 recrystallisation Methods 0.000 claims abstract description 17
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 13
- 230000007935 neutral effect Effects 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 238000004132 cross linking Methods 0.000 claims abstract description 6
- 230000003110 anti-inflammatory effect Effects 0.000 claims abstract description 4
- 230000006698 induction Effects 0.000 claims description 45
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 30
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 claims description 24
- 210000004087 cornea Anatomy 0.000 claims description 12
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 8
- 229920000669 heparin Polymers 0.000 claims description 8
- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 claims description 5
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 4
- HZFGMQJYAFHESD-UHFFFAOYSA-M bromfenac sodium Chemical compound [Na+].NC1=C(CC([O-])=O)C=CC=C1C(=O)C1=CC=C(Br)C=C1 HZFGMQJYAFHESD-UHFFFAOYSA-M 0.000 claims description 4
- 229960002716 bromfenac sodium Drugs 0.000 claims description 4
- 229960002897 heparin Drugs 0.000 claims description 4
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims description 4
- 229960001008 heparin sodium Drugs 0.000 claims description 4
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims description 3
- 229930182566 Gentamicin Natural products 0.000 claims description 3
- 229960002518 gentamicin Drugs 0.000 claims description 3
- 238000002834 transmittance Methods 0.000 abstract description 13
- 230000015556 catabolic process Effects 0.000 abstract description 7
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- 229920002554 vinyl polymer Polymers 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- 238000011369 optimal treatment Methods 0.000 description 2
- 238000001878 scanning electron micrograph Methods 0.000 description 2
- 201000004569 Blindness Diseases 0.000 description 1
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010038848 Retinal detachment Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- ZBPLOVFIXSTCRZ-UHFFFAOYSA-N bromfenac Chemical compound NC1=C(CC(O)=O)C=CC=C1C(=O)C1=CC=C(Br)C=C1 ZBPLOVFIXSTCRZ-UHFFFAOYSA-N 0.000 description 1
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- 239000000377 silicon dioxide Substances 0.000 description 1
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- 230000003075 superhydrophobic effect Effects 0.000 description 1
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- C08J2329/02—Homopolymers or copolymers of unsaturated alcohols
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Abstract
The invention provides a preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material and the prepared artificial eye tissue engineering substitute material, belonging to the technical field of eye tissue engineering repair, the preparation method of the polyvinyl alcohol artificial eye tissue engineering substitute material comprises the following steps: preparing polyvinyl alcohol hydrogel by using a polyvinyl alcohol solution as a raw material and adopting a physical crosslinking method; placing polyvinyl alcohol hydrogel in an eye tissue mold for limited-area hot pressing treatment to shape and dry the polyvinyl alcohol hydrogel, and then soaking the polyvinyl alcohol hydrogel in an inducing solvent for inducing recrystallization to obtain a substitute material prototype; and dialyzing the embryonic form of the substitute material to be neutral, and then loading functional active ingredients with antibacterial or anti-inflammatory effects to obtain the artificial eye tissue engineering substitute material. The artificial ocular tissue engineering substitute material prepared by the invention is acid and alkali resistant, swelling resistant, degradation resistant, antibacterial, excellent in mechanical property and light transmittance, and has a good application prospect in the ocular tissue engineering repair field.
Description
Technical Field
The invention belongs to the technical field of eye tissue engineering repair, and particularly relates to a preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material and the prepared artificial eye tissue engineering substitute material.
Background
Cataract and retinal detachment caused by diseases or external injuries are the primary factors causing blindness of patients all over the world, and the conventional treatment means is mainly replacement implantation of artificial eye tissue engineering materials. Wherein, the artificial lens and the artificial retina are medical instruments for eye tissue engineering which are widely applied clinically. At present, the commonly used eye tissue engineering substitutes for medical instruments and instruments which are mainly classified into silica gels, polymethacrylates, polyhydroxyethylmethacrylate and the like. However, the natural physiological microenvironment of the human eye is fragile and complex, especially with stringent requirements for light transmittance of alternative materials. In addition, the antibacterial property, biocompatibility, degradation resistance, low inflammatory reactivity and anti-biological macromolecular adhesion of the substitute material also become main standards for investigating the timeliness of the medical instruments.
Based on the above-mentioned industrial problems, the current practice is mainly to carry out the attack and the customs based on the following strategies: (1) The surface modification is carried out on the prior artificial lens, and the problems are solved by adjusting functional groups and surface microstructures; (2) The functions and effects are realized by loading effective active ingredients in situ; (3) The eye tissue engineering substitute material is directly prepared by adopting an antibacterial or super-hydrophilic and super-hydrophobic material so as to realize the functions. However, a single processing measure often cannot ensure the integration of multiple necessary functions.
Therefore, from the current industry strategy, realizing the synergistic enhancement of the key factors of high strength, high transparency, antibiosis, adhesion prevention and degradation resistance still remains a great problem to be solved clinically.
Disclosure of Invention
Aiming at the technical problems, the invention provides a preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material and the prepared artificial eye tissue engineering substitute material.
In order to achieve the purpose, the invention adopts the technical scheme that:
the invention provides a preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material, which comprises the following steps:
preparing polyvinyl alcohol hydrogel by using a polyvinyl alcohol solution as a raw material and adopting a physical crosslinking method;
placing polyvinyl alcohol hydrogel in an eye tissue mold for limited-area hot pressing treatment to shape and dry the polyvinyl alcohol hydrogel, and then soaking the polyvinyl alcohol hydrogel in an inducing solvent for inducing recrystallization to obtain a substitute material prototype;
and dialyzing the prototype of the substitute material to be neutral, and then loading functional active ingredients with antibacterial or anti-inflammatory effects to obtain the artificial eye tissue engineering substitute material.
In some of these embodiments, the concentration of the polyvinyl alcohol solution is 0.1% or greater.
In some of these examples, the polyvinyl alcohol hydrogel is prepared using solvent induction.
In some embodiments, the inducing solvent used for preparing the polyvinyl alcohol hydrogel and the inducing solvent used for inducing recrystallization are selected from any one of sodium hydroxide solution, potassium hydroxide solution and lithium hydroxide solution.
In some embodiments, the polyvinyl alcohol hydrogel is prepared and subjected to induced recrystallization treatment at the temperature of-10-40 ℃ for 0.5-24 h.
In some of these embodiments, the mold of ocular tissue is selected from any one of a lens mold, a corneal mold, a hollow tubular mold, a mesh mold, and a microneedle array mold.
In some embodiments, the hot-pressing temperature is 40-200 ℃, and the hot-pressing time is 0.5-24 h.
In some of these embodiments, the functionally active ingredient is selected from any one of heparin, heparin sodium, penicillin sodium, gentamicin, and bromfenac sodium.
In some embodiments, the specific steps of loading the functional active ingredient are: soaking the dialyzed embryonic form of the substitute material in a solution containing functional active ingredients; the concentration of the functional active ingredients in the solution is 0.05-5%, and the soaking time is 1-24 h.
The invention also provides a polyvinyl alcohol artificial eye tissue engineering substitute material which is prepared by the preparation method of the polyvinyl alcohol artificial eye tissue engineering substitute material according to any one technical scheme.
Compared with the prior art, the invention has the advantages and positive effects that:
1. the preparation method of the polyvinyl alcohol artificial eye tissue engineering substitute material provided by the invention adopts polyvinyl alcohol with good biocompatibility and degradability as a raw material, adopts a physical cross-linking method to prepare the polyvinyl alcohol hydrogel with the super-hydrophilic surface, utilizes an eye tissue mold to obtain substitute material prototypes with different appearances in a limited domain-hot pressing mode, and further combines the in-situ load of functional active ingredients, so that the strength, antibacterial property, degradation resistance and adhesion resistance of the artificial eye tissue engineering substitute material are synergistically enhanced;
2. the preparation method of the polyvinyl alcohol artificial eye tissue engineering substitute material provided by the invention has the advantages of easily available raw materials, simple preparation process and low preparation cost, and the prepared artificial eye tissue engineering substitute material has good application prospect in the field of eye tissue engineering repair and has potential as an eye medical apparatus;
3. the polyvinyl alcohol artificial eye tissue engineering substitute material provided by the invention is acid-base resistant, swelling resistant, degradation resistant, antibacterial, excellent in mechanical property and light transmittance, and has a good application prospect in the field of eye tissue engineering repair.
Drawings
FIG. 1 is a schematic representation of the placement of a polyvinyl alcohol hydrogel in a lens mold as provided in example 1 of the present invention;
FIG. 2 is a schematic diagram showing the appearance of the substitute material for artificial eye tissue engineering provided in example 1 of the present invention;
FIG. 3 is an SEM image of an artificial eye tissue engineering substitute material provided in example 1 of the present invention;
FIG. 4 is a graph showing the results of the transmittance test of the substitute material for artificial eye tissue engineering provided in example 1 of the present invention;
FIG. 5 is a graph showing the results of tensile testing of the substitute material for artificial eye tissue engineering provided in example 1 of the present invention;
fig. 6 is a surface contact angle test result chart of the artificial eye tissue engineering substitute material provided in embodiment 1 of the present invention.
In the figure, the position of the first and second end faces,
1. a lens mold; 11. a lower die; 111. a hemispherical recess; 12. an upper die; 2. polyvinyl alcohol hydrogels.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
The embodiment of the invention provides a preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material, which comprises the following steps:
s1: preparing polyvinyl alcohol hydrogel by using a polyvinyl alcohol solution as a raw material and adopting a physical crosslinking method;
s2: placing polyvinyl alcohol hydrogel in an eye tissue mold for limited-area hot pressing treatment to shape and dry the polyvinyl alcohol hydrogel, and then soaking the polyvinyl alcohol hydrogel in an inducing solvent for inducing recrystallization to obtain a substitute material prototype;
s3: and dialyzing the prototype of the substitute material to be neutral, and then loading functional active ingredients with antibacterial or anti-inflammatory effects to obtain the artificial eye tissue engineering substitute material.
The preparation method of the polyvinyl alcohol artificial eye tissue engineering substitute material adopts polyvinyl alcohol with good biocompatibility and degradability as a raw material, adopts a physical crosslinking method to prepare polyvinyl alcohol hydrogel with super-hydrophilic performance on the surface, utilizes an eye tissue mold to obtain substitute material prototypes with different shapes in a limited domain-hot pressing mode, and further combines the in-situ load of functional active ingredients, so that the strength, the antibacterial property, the degradation resistance and the anti-adhesion performance of the artificial eye tissue engineering substitute material are synergistically enhanced, the light transmittance of the obtained artificial eye tissue engineering substitute material is higher than 85%, the strength is 5 MPa-50 MPa, and the surface contact angle is lower than 10 degrees. Meanwhile, the preparation method of the polyvinyl alcohol artificial eye tissue engineering substitute material has the advantages of easily available raw materials, simple preparation process and low preparation cost, and the prepared artificial eye tissue engineering substitute material has good application prospect in the field of eye tissue engineering repair and has the potential of being used as an eye medical appliance.
In some of these embodiments, the concentration of the polyvinyl alcohol solution is 0.1% or greater. In this example, the lower limit of the concentration of the polyvinyl alcohol solution is given, and when the concentration is lower than the lower limit, the tensile strength of the polyvinyl alcohol hydrogel cannot be ensured.
In some of these examples, the polyvinyl alcohol hydrogel is prepared using a solvent-induced method. In the embodiment, the preferable preparation method of the polyvinyl alcohol hydrogel is provided, the polyvinyl alcohol hydrogel is prepared by a solvent induction method, the steps are simple, the preparation cost is low, and the prepared polyvinyl alcohol hydrogel has super-hydrophilic property, high transparency and good mechanical property, and is favorable for obtaining the artificial eye tissue engineering substitute material with excellent performance through subsequent treatment. The solvent induction method is a common method for preparing polyvinyl alcohol hydrogel, and includes two methods, namely directly inducing a polyvinyl alcohol solution to prepare the polyvinyl hydrogel and inducing a polyvinyl alcohol film to prepare the polyvinyl hydrogel, wherein the former induction method is to place the polyvinyl alcohol solution in an induction solvent for induction, and the latter induction method is to immerse the polyvinyl alcohol film in the induction solvent for induction, wherein when the induced polyvinyl alcohol film is used for preparing the polyvinyl hydrogel, the used film is prepared by adopting a flow casting-drying method, the drying temperature is 45 ℃, the flow casting-drying method is a common method for preparing the polyvinyl alcohol film in the field, and specific preparation steps are not repeated herein.
In some embodiments, the inducing solvent used for preparing the polyvinyl alcohol hydrogel and the inducing solvent used for inducing recrystallization are selected from any one of sodium hydroxide solution, potassium hydroxide solution and lithium hydroxide solution. In the embodiment, the preferable types of the inducing solvents are given, and the preferable inducing solvents are favorable for preparing and obtaining the polyvinyl alcohol hydrogel with super-hydrophilic property, high transparency and good mechanical property. The concentration of the sodium hydroxide solution is preferably 5% to 50%, the concentration of the potassium hydroxide solution is preferably 5% to 60%, and the concentration of the lithium hydroxide solution is preferably 5% to 15%.
In some embodiments, the polyvinyl alcohol hydrogel is prepared and subjected to induced recrystallization, wherein the induction temperature is-10-40 ℃, and the induction time is 0.5-24 hours. In the embodiment, a preferable induction temperature range and an induction time range are given, and the preferable induction conditions are adopted, so that the polyvinyl alcohol hydrogel with super-hydrophilic property, high transparency and good mechanical property can be prepared and obtained.
In some of these embodiments, the mold of ocular tissue is selected from any one of a lens mold, a corneal mold, a hollow tubular mold, a mesh mold, and a microneedle array mold. By adopting different eye tissue molds, artificial eye tissue engineering substitute materials with different appearances can be prepared and obtained. It should be noted that the lens mold, the cornea mold, the hollow tubular mold, the mesh mold and the microneedle array mold are all existing ocular tissue molds.
In some embodiments, the hot-pressing temperature is 40-200 ℃, and the hot-pressing time is 0.5-24 h. In the embodiment, the optimal treatment conditions for the hot pressing treatment are given, and the optimal treatment conditions are adopted, so that the morphology and the mechanical property of the artificial eye tissue engineering substitute material can be guaranteed.
In some of these embodiments, the functionally active ingredient is selected from any one of heparin, sodium penicillin, gentamicin, and sodium bromfenac. In the embodiment, the preferable functional active ingredients are provided, and the functional active ingredients are loaded, so that the antibacterial property of the substitute material is improved.
In some embodiments, the specific steps of loading the functional active ingredient are: soaking the dialyzed embryonic substitute material in a solution containing functional active ingredients; the concentration of the functional active ingredients in the solution is 0.05-5%, and the soaking time is 1-24 h. In the embodiment, a preferable mode of loading functional active ingredients is provided, which is beneficial to ensuring the antibacterial property of the artificial eye tissue engineering substitute material.
The embodiment of the invention also provides a polyvinyl alcohol artificial eye tissue engineering substitute material which is prepared by the preparation method of the polyvinyl alcohol artificial eye tissue engineering substitute material. The polyvinyl alcohol artificial eye tissue engineering substitute material is acid-base resistant, swelling resistant, degradation resistant and antibacterial, has excellent mechanical property and light transmittance, and has good application prospect in the field of eye tissue engineering repair.
In order to more clearly and specifically describe the preparation method of the polyvinyl alcohol artificial eye tissue engineering substitute material and the artificial eye tissue engineering substitute material prepared by the preparation method, the following description will be made with reference to specific examples.
Example 1
A preparation method of polyvinyl alcohol artificial eye tissue engineering substitute material comprises the following steps:
(1) Dissolving polyvinyl alcohol in deionized water at 85 ℃ to prepare a polyvinyl alcohol solution with the concentration of 5%, drying the polyvinyl alcohol solution at 45 ℃ to form a film, then soaking the film in a sodium hydroxide solution with the concentration of 50% to induce the film to form polyvinyl alcohol hydrogel, wherein the induction temperature is-10 ℃, and the induction time is 24 hours;
(2) As shown in fig. 1, placing the polyvinyl alcohol hydrogel 2 obtained in the step (1) in a hemispherical groove 111 of a lower mold 11 of a lens mold 1, then pressing by using a hemispherical upper mold 12, performing limited hot pressing treatment to shape and dry the polyvinyl alcohol hydrogel, wherein the hot pressing treatment temperature is 200 ℃, the hot pressing treatment time is 0.1h, then soaking the obtained material in 5% potassium hydroxide solution for induced recrystallization, the induction temperature is 40 ℃, and the induction time is 0.5h, so as to obtain a prototype of the lens substitute material;
(3) Dialyzing the embryonic lens substitute material obtained in the step (2) in deionized water to be neutral, and soaking in a heparin solution with the concentration of 0.05% for 24 hours to obtain the lens substitute material, wherein the appearance of the lens substitute material is shown in figure 2, and the SEM image of the lens substitute material is shown in figure 3, so that the inside of the lens substitute material is a porous structure. As shown in fig. 4 to 6, the lens-replacing material prepared was tested to have a light transmittance of 87%, an intensity of 28MPa, and a surface contact angle of 8 °.
Example 2
A preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material comprises the following steps:
(1) Dissolving polyvinyl alcohol in deionized water at 85 ℃ to prepare a 15% polyvinyl alcohol solution, placing the polyvinyl alcohol solution in a 60% potassium hydroxide solution to induce to form polyvinyl alcohol hydrogel, wherein the induction temperature is 40 ℃, and the induction time is 12 hours;
(2) Placing the polyvinyl alcohol hydrogel obtained in the step (1) in a cornea mold for limited-area hot-pressing treatment to shape and dry the polyvinyl alcohol hydrogel, wherein the hot-pressing treatment temperature is 50 ℃, the hot-pressing treatment time is 24 hours, then soaking the obtained material in a sodium hydroxide solution with the concentration of 5% for induced recrystallization, the induction temperature is 4 ℃, and the induction time is 10 hours, so as to obtain a cornea substitute material prototype;
(3) Dialyzing the cornea substitute material prototype obtained in the step (2) in deionized water to be neutral, soaking in a heparin sodium solution with the concentration of 5% for 12 hours to obtain the cornea substitute material, and testing that the cornea substitute material has the light transmittance of 92%, the strength of 12MPa and the surface contact angle of 5 degrees.
Example 3
A preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material comprises the following steps:
(1) Dissolving polyvinyl alcohol in deionized water at 85 ℃ to prepare a polyvinyl alcohol solution with the concentration of 30%, and placing the polyvinyl alcohol solution in a lithium hydroxide solution with the concentration of 15% to induce to form polyvinyl alcohol hydrogel, wherein the induction temperature is 10 ℃, and the induction time is 4 hours;
(2) Placing the polyvinyl alcohol hydrogel obtained in the step (1) in a hollow tubular mold for limited hot pressing treatment to shape and dry the polyvinyl alcohol hydrogel, wherein the hot pressing treatment temperature is 100 ℃, the hot pressing treatment time is 12 hours, then soaking the obtained material in a sodium hydroxide solution with the concentration of 30% for induced recrystallization, the induction temperature is 15 ℃, and the induction time is 12 hours, so as to obtain a substitute material prototype with the hollow tubular shape;
(3) And (3) dialyzing the prototype of the hollow tubular shape substitute material obtained in the step (2) in deionized water to be neutral, soaking in a 1% bromfenac sodium solution for 1h to obtain the hollow tubular shape substitute material, and testing that the light transmittance is 95%, the strength is 5MPa and the surface contact angle is 6 degrees.
Example 4
A preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material comprises the following steps:
(1) Dissolving polyvinyl alcohol in deionized water at 85 ℃ to prepare a polyvinyl alcohol solution with the concentration of 10%, drying the polyvinyl alcohol solution at 45 ℃ to form a film, then soaking the film in a sodium hydroxide solution with the concentration of 40% to induce the film to form polyvinyl alcohol hydrogel, wherein the induction temperature is-10 ℃, and the induction time is 0.5h;
(2) Placing the polyvinyl alcohol hydrogel obtained in the step (1) in a microneedle array mould for limited hot-pressing treatment to shape and dry the polyvinyl alcohol hydrogel, wherein the hot-pressing treatment temperature is 150 ℃, the hot-pressing treatment time is 8h, then soaking the obtained material in a lithium hydroxide solution with the concentration of 5% for induced recrystallization, the induction temperature is 30 ℃, and the induction time is 20h, so as to obtain a substitute material prototype with the microneedle array morphology;
(3) And (3) dialyzing the prototype of the substitute material with the shape of the microneedle array obtained in the step (2) in deionized water to be neutral, soaking in a penicillin sodium solution with the concentration of 2% for 8 hours to obtain the substitute material with the shape of the microneedle array, wherein the test shows that the substitute material has the light transmittance of 90%, the strength of 40MPa and the surface contact angle of 7 degrees.
Example 5
A preparation method of polyvinyl alcohol artificial eye tissue engineering substitute material comprises the following steps:
(1) Dissolving polyvinyl alcohol in deionized water at 85 ℃ to prepare a 0.1% polyvinyl alcohol solution, drying the polyvinyl alcohol solution at 45 ℃ to form a film, then soaking the film in a 5% sodium hydroxide solution to induce the film to form polyvinyl alcohol hydrogel, wherein the induction temperature is-4 ℃, and the induction time is 3 hours;
(2) Placing the polyvinyl alcohol hydrogel obtained in the step (1) in a microneedle array mould for limited-area hot pressing treatment to shape and dry the polyvinyl alcohol hydrogel, wherein the hot pressing treatment temperature is 100 ℃, the hot pressing treatment time is 6 hours, then soaking the obtained material in a sodium hydroxide solution with the concentration of 50% for induced recrystallization, the induction temperature is-10 ℃, and the induction time is 24 hours, so as to obtain a prototype of the lens substitute material;
(3) And (3) dialyzing the prototype of the substitute material with the shape of the microneedle array obtained in the step (2) in deionized water to be neutral, soaking in a penicillin sodium solution with the concentration of 1.5% for 4 hours to obtain the substitute material with the shape of the microneedle array, and testing the substitute material with the shape of the microneedle array, wherein the light transmittance is 91%, the strength is 20MPa, and the surface contact angle is 9 degrees.
Example 6
A preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material comprises the following steps:
(1) Dissolving polyvinyl alcohol in deionized water at 85 ℃ to prepare a polyvinyl alcohol solution with the concentration of 12%, and placing the polyvinyl alcohol solution in a potassium hydroxide solution with the concentration of 5% to induce to form polyvinyl alcohol hydrogel, wherein the induction temperature is 30 ℃, and the induction time is 10 hours;
(2) Placing the polyvinyl alcohol hydrogel obtained in the step (1) in a cornea mold for limited hot pressing treatment to shape and dry the polyvinyl alcohol hydrogel, wherein the hot pressing treatment temperature is 80 ℃, the hot pressing treatment time is 20 hours, then soaking the obtained material in a potassium hydroxide solution with the concentration of 60% for induced recrystallization, the induction temperature is 12 ℃, and the induction time is 16 hours, so as to obtain a cornea substitute material prototype;
(3) Dialyzing the cornea substitute material prototype obtained in the step (2) in deionized water to be neutral, soaking in heparin sodium solution with the concentration of 3% for 10 hours to obtain the cornea substitute material, and testing that the cornea substitute material has the light transmittance of 91%, the strength of 10MPa and the surface contact angle of 12 degrees.
Example 7
A preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material comprises the following steps:
(1) Dissolving polyvinyl alcohol in deionized water at 85 ℃ to prepare a polyvinyl alcohol solution with the concentration of 25%, and placing the polyvinyl alcohol solution in a lithium hydroxide solution with the concentration of 5% to induce to form polyvinyl alcohol hydrogel, wherein the induction temperature is 25 ℃, and the induction time is 15 hours;
(2) Placing the polyvinyl alcohol hydrogel obtained in the step (1) in a hollow tubular mold for limited hot pressing treatment to shape and dry the polyvinyl alcohol hydrogel, wherein the hot pressing treatment temperature is 120 ℃, the hot pressing treatment time is 18h, then soaking the obtained material in a lithium hydroxide solution with the concentration of 15% for induced recrystallization, the induction temperature is 18 ℃, and the induction time is 18h, so as to obtain a substitute material prototype with the hollow tubular shape;
(3) And (3) dialyzing the prototype of the hollow tubular shape substitute material obtained in the step (2) in deionized water to be neutral, soaking in 0.5% bromfenac sodium solution for 5h to obtain the hollow tubular shape substitute material, and testing to obtain the hollow tubular shape substitute material with the light transmittance of 94%, the strength of 8MPa and the surface contact angle of 8 degrees.
Claims (10)
1. A preparation method of a polyvinyl alcohol artificial eye tissue engineering substitute material is characterized by comprising the following steps:
preparing polyvinyl alcohol hydrogel by using a polyvinyl alcohol solution as a raw material and adopting a physical crosslinking method;
placing the polyvinyl alcohol hydrogel in an eye tissue mold for limited-area hot-pressing treatment to shape and dry the polyvinyl alcohol hydrogel, and then immersing the polyvinyl alcohol hydrogel in an inducing solvent for induced recrystallization to obtain a substitute material prototype;
and dialyzing the embryonic form of the substitute material to be neutral, and then loading functional active ingredients with antibacterial or anti-inflammatory effects to obtain the artificial eye tissue engineering substitute material.
2. The method for preparing the polyvinyl alcohol artificial eye tissue engineering substitute material according to claim 1, wherein the concentration of the polyvinyl alcohol solution is 0.1% or more.
3. The method for preparing the polyvinyl alcohol artificial eye tissue engineering substitute material according to claim 1, wherein the polyvinyl alcohol hydrogel is prepared by a solvent induction method.
4. The method for preparing a polyvinyl alcohol artificial eye tissue engineering substitute material according to claim 1 or 3, wherein the inducing solvent used for preparing the polyvinyl alcohol hydrogel and the inducing solvent used for the induced recrystallization treatment are selected from any one of a sodium hydroxide solution, a potassium hydroxide solution and a lithium hydroxide solution.
5. The method for preparing the polyvinyl alcohol artificial eye tissue engineering substitute material according to claim 4, wherein the induction temperature and the induction time are both-10 ℃ to 40 ℃ and 0.5h to 24h, respectively, during the preparation of the polyvinyl alcohol hydrogel and the induced recrystallization.
6. The method for preparing a PVA artificial eye tissue engineering substitute material according to claim 1, wherein the eye tissue mold is selected from any one of a lens mold, a cornea mold, a hollow tubular mold, a mesh mold and a microneedle array mold.
7. The method for preparing the polyvinyl alcohol artificial eye tissue engineering substitute material according to claim 1, wherein the hot-pressing temperature is 40 ℃ to 200 ℃, and the hot-pressing time is 0.5h to 24h.
8. The method for preparing a polyvinyl alcohol artificial eye tissue engineering substitute material according to claim 1, wherein the functional active ingredient is selected from any one of heparin, heparin sodium, penicillin sodium, gentamicin and bromfenac sodium.
9. The method for preparing the polyvinyl alcohol artificial eye tissue engineering substitute material according to claim 1, wherein the specific steps of loading the functional active ingredients are as follows: soaking the dialyzed embryonic substitute material in a solution containing functional active ingredients; the concentration of the functional active ingredients in the solution is 0.05-5%, and the soaking time is 1-24 h.
10. A polyvinyl alcohol artificial eye tissue engineering substitute material, characterized by being prepared by the method for preparing the polyvinyl alcohol artificial eye tissue engineering substitute material according to any one of claims 1 to 9.
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