CN115624573B

CN115624573B - Probiotic composition for improving hair health condition and application thereof

Info

Publication number: CN115624573B
Application number: CN202211652274.XA
Authority: CN
Inventors: 张文朋; 金庭飞; 陶纯长
Original assignee: Guangdong Yike Weisheng Technology Co ltd
Current assignee: Guangdong Yike Weisheng Technology Co ltd
Priority date: 2022-12-22
Filing date: 2022-12-22
Publication date: 2023-04-18
Anticipated expiration: 2042-12-22
Also published as: CN115624573A

Abstract

The invention discloses a probiotic composition for improving the health condition of hair and application thereof, wherein the probiotic composition comprises one or two of bifidobacterium bifidum Y22 and lactobacillus fermentum FM-12; and, one or two combinations including lactobacillus rhamnosus YGG and lactobacillus reuteri K07. The probiotic composition for improving the health condition of the hair has good effects of promoting hair growth, fixing the hair, preventing alopecia, improving the strength of the hair and increasing the elasticity of the hair. The probiotic freeze-dried powder can be applied to preparing various functional products, including probiotic freeze-dried powder, probiotic lysate, probiotic traditional Chinese medicine fermentation product and the like; the product can be an oral preparation or an external preparation, and has wide application prospect.

Description

Probiotic composition for improving hair health condition and application thereof

Technical Field

The invention relates to the technical field of probiotics, in particular to a probiotic composition for improving the health condition of hair and application thereof.

Background

Alopecia is a physiological phenomenon caused by the fact that the number of new hairs on the scalp is less than the number of fallen hairs, hair is thinned for a long time, and then partial or all areas of the scalp have no hairs, namely complete alopecia is caused. Although the hair loss does not affect the health of the body, the hair loss greatly affects the beauty of the body, and particularly causes great trouble to the body and the heart of people in the current 'exterior-emphasized' age.

Factors that lead to hair loss are: seasonal alopecia, neurogenic alopecia, chemical alopecia, physical alopecia, lipoid alopecia (androgenetic alopecia), and the like, and among these factors, lipoid alopecia (androgenetic alopecia) and neurogenic alopecia are the most common.

The neurogenic alopecia is related to stress, and the excessive stress can cause physical and mental fatigue, liver fire and liver depression. According to the traditional Chinese medicine, the liver depression hurts the spleen, namely the liver takes the spleen (liver wood is used for tonifying spleen earth), and the hair loss is further caused after the spleen and the stomach are weak.

Seborrheic alopecia (seborrhoeic alopecia, SA) is also called androgenetic alopecia, and is called as 'carious alopecia' and 'decayed hair tinea' in traditional Chinese medicine. The common skin damage of the disease is the top of the head or M-type progressive alopecia of the forehead, and the plain scalp is itchy, oily and dandruff. Seborrheic alopecia is divided into acute and chronic. In the acute stage, the scalp is oiled, or dandruff or pimple, or itch, and the hair is scooped up and falls off in a short time and even completely fades. The scalp in the chronic period is greasy and shiny, is oiled, has a large amount of grey bran-like dandruff, is dry, lacks luster, has severe pruritus, and mostly slowly falls off from the two sides of the forehead and the top of the head, and baldness is formed after years or decades, but the alopecia totalis is not easy to form.

The western medicine considers that the causes of seborrheic alopecia are complex and related to genetic factors, endocrine dyscrasia, emotion, autoimmunity and the like, but the main related factors are androgen metabolism disorder and are closely related to the levels of testosterone and a metabolite Dihydrotestosterone (DHT) thereof, 5 alpha-reductase of follicular unit and Androgen Receptor (AR). Only two drugs, finasteride and minoxidil, have received acceptance by the united states Food and Drug Administration (FDA) (u.s.a) as alopecia therapeutics to date. However, because of unpredictable effect and side effect, and long-term administration is needed, the alopecia is more serious after the medicine is stopped, and the use is limited.

The traditional Chinese medicine recognizes alopecia earlier, and considers that hair growth and metabolism are originated from viscera, originated from essence and blood, and are honored in meridians and collaterals, and are longer than skin striae. The record of Huangdi's classic on medicine: the excessive blood-qi can lead to the strong kidney-qi, so it turns black; deficiency of blood-qi can lead to kidney-qi deficiency, leading to white hair and loss, suggesting that hair loss is related to kidney-qi and blood-qi. Wang Qingren, yi Lin correction records that the hair falls off and all medical books are concerned with blood damage. Lingshu-Yin twenty five people cloud: "blood and qi are less, without hair, and rare and withered if there is blood and qi. The theory of the etiology of various diseases and the hair loss state are recorded in the book: if blood is abundant, the hair will be lustered, so the beard and hair will be beautiful; if the blood and qi are weakened, the meridians are exhausted and unable to nourish, so the beard and hair fall off. Plain questions and five zang organs generation cloud: the kidney also binds to the bone, it also nourishes hair, and it also governs spleen. Therefore, it is \8230, and if it is eaten more often, it will cause bone pain and fall. According to the above classic, the hair is rich and moist and closely related to qi and blood. The spleen is the source of the generation and transformation of qi and blood, and is the acquired root, and the function of the spleen in transportation and transformation can make qi and blood up to the vertex, thereby nourishing hair, and making hair glossy and tough. The students think that spleen earth is healthy and healthy for ever, one of the spleen earth can generate qi and blood and cultivate the origin of hair growth, and roots of all things are planted in the earth, which is the 'earth grows all things'; secondly, the lung governs skin and hair adheres to the skin, and grows by means of lung qi diffusing and moistening, so that the lung can be fumigated and hair can be moisturized by virtue of hilling; thirdly, the hair can tonify the innate essence in the acquired, the spleen and earth are healthy and strong, the acquired essence is strong, and the innate essence can tonify the innate essence, so that the kidney essence is full and the hair is luxuriant.

Therefore, no matter what the liver and kidney are responsible for, it is the essential method for promoting hair growth, strengthening and consolidating hair, preventing alopecia by the way of spleen-earth, transporting and transforming qi and blood.

The probiotics has the powerful effect of conditioning the gastrointestinal tract, promotes the nutrient absorption and keeps the gastrointestinal tract healthy by regulating the host mucous membrane and system immune function or regulating the flora balance in the intestinal tract, has the probiotic function, has the functions of promoting the digestion and absorption of nutrient substances, improving the immunity of the organism, maintaining the intestinal tract flora structure balance, inhibiting intestinal inflammation, protecting the intestinal tract mucous membrane barrier and the like, is related to the functions of the spleen and the stomach in the traditional Chinese medicine, and promotes the functions and the effects, namely, the functions are equivalent to the functions of strengthening and transporting the spleen and the stomach in the traditional Chinese medicine. Therefore, from the perspective of traditional Chinese medicine, the probiotics has the function of strengthening and transporting the spleen and stomach.

Therefore, the method has the advantages of screening and optimizing probiotics and the combination thereof, strengthening the spleen and stomach, and transporting and transforming qi and blood to achieve the effects of promoting hair growth, fixing hair and preventing alopecia, and is a feasible and innovative direction.

Patent CN110325194A discloses a composition containing fermentation product of aquatic animals for preventing alopecia and promoting hair growth, which contains lactobacillus and yeast fermentation product of aquatic animals or aquatic animal processing by-products as effective ingredients, wherein the lactobacillus includes lactobacillus acidophilus, pediococcus pentosaceus, pediococcus cerevisiae, etc. The composition has effects of inhibiting and preventing alopecia and promoting hair growth, but the disclosed strain is many and complicated, and is not clear and optimized. And the samples used in the implementation examples are fermented fish oil. The preparation process of the fermented fish oil comprises the following steps: adding Lactobacillus plantarum and Saccharomyces cerevisiae, fermenting, extracting fish oil with hexane, and recovering hexane to obtain fermented fish oil. Thus, the product is free of active probiotics and is also substantially free of probiotic or metabolite components thereof (little fat soluble components will be incorporated into the fish oil). Therefore, it is not confirmed that the effective ingredients for preventing hair loss and promoting hair growth include probiotics.

The market lacks a safe and healthy probiotic product which can be orally taken or/and externally applied and has better effects of promoting hair growth, fixing hair and preventing alopecia.

Disclosure of Invention

In order to overcome the disadvantages of the prior art, it is an object of the present invention to provide a probiotic composition for improving the health of hair.

The invention also aims to provide application of the probiotic composition for improving the health condition of hair.

One of the purposes of the invention is realized by adopting the following technical scheme:

a probiotic composition for improving hair health comprises one or two of Bifidobacterium bifidum and Lactobacillus fermentum; and, one or a combination of two of lactobacillus rhamnosus and lactobacillus reuteri; the bifidobacterium bifidum is bifidobacterium bifidum Y22 with the preservation number of CGMCC NO. 15708; the lactobacillus fermentum is lactobacillus fermentum FM-12 with the preservation number of GDMCC NO. 62045; the lactobacillus rhamnosus is Lactobacillus rhamnosus YGG with the preservation number of CGMCC NO. 15027; the lactobacillus reuteri is lactobacillus reuteri K07 with the preservation number of CGMCC NO. 15703.

In the probiotic composition for improving the health status of hair according to the present invention, the bifidobacterium bifidum Y22 and the lactobacillus fermentum FM-12 are oil controlling strains, and the lactobacillus rhamnosus YGG and the lactobacillus reuteri K07 are anti-inflammatory strains. The oil-controlling strain and the oil-resistant strain take effect synergistically and improve the health condition of hair together, wherein 4 strains are compounded, so that the synergistic effect is most remarkable.

The bifidobacterium bifidum Y22 is one of the first members in the construction of intestinal microbial flora of early infants, has the advantages of acid resistance, bile salt resistance, oxygen resistance and strong intestinal colonization capacity, can balance the intestinal flora, and reduces the absorption of finger fat by a digestive system, thereby reducing the amount of scalp fat, restoring the scalp environment and reducing alopecia caused by vigorous fat secretion.

The lactobacillus fermentum FM-12 has strong acid resistance, has an inhibiting effect on various pathogenic bacteria, can balance intestinal flora, reduce TG and TC contents, reduce blood fat and cholesterol contents in vivo, radically reduce scalp grease secretion, improve scalp environment and reduce alopecia.

The lactobacillus rhamnosus YGG has strong acid resistance, cholate resistance and intestinal tract colonization capacity, can reduce the quantity of intestinal harmful bacteria, improve the intestinal tract environment, reduce intestinal tract virulence factors, reduce the occurrence of body inflammation, improve the folliculitis inflammation symptoms, and is beneficial to recovering the scalp environment and recovering the health of hair follicle cells.

Lactobacillus reuteri K07, which is acid and bile salt resistant, can produce short chain fatty acids (such as butyric acid) to promote the proliferation of intestinal epithelial cells and facilitate the repair of intestinal mucosa; can produce bacteriostatic substance (Roy's element), inhibit the growth of harmful bacterium; can synthesize vitamin B group, convert inorganic selenium into organic selenium for organism, has obvious antioxidation effect, is beneficial to repair inflammatory cells, improves folliculitis inflammatory symptoms, and is beneficial to recovering scalp environment and recovering hair follicle cells to be healthy.

The four strains are acid-resistant and cholate-resistant strains, have strong capability of colonizing and growing in gastrointestinal tracts, can promote the health of the gastrointestinal tracts, and jointly play the effects of strengthening and activating spleen soil, and generating qi and blood so as to promote hair growth, fix hair and prevent alopecia.

As a preferred embodiment of the present invention, the probiotic composition comprises a combination of the bifidobacterium bifidum Y22 and the lactobacillus rhamnosus YGG.

As a preferred embodiment of the present invention, said probiotic composition comprises a combination of said bifidobacterium bifidum Y22 and said lactobacillus reuteri K07.

As a preferred embodiment of the present invention, the probiotic composition comprises a combination of the lactobacillus fermentum FM-12 and the lactobacillus rhamnosus YGG.

As a preferred embodiment of the present invention, the probiotic composition comprises a combination of said lactobacillus fermentum FM-12 and said lactobacillus reuteri K07.

As a preferred embodiment of the present invention, the probiotic composition comprises a combination of the bifidobacterium bifidum Y22, the lactobacillus rhamnosus YGG, and the lactobacillus reuteri K07.

As a preferred embodiment of the present invention, the probiotic composition comprises a combination of the lactobacillus fermentum FM-12, the lactobacillus rhamnosus YGG, and the lactobacillus reuteri K07.

In a preferred embodiment of the present invention, the probiotic composition comprises a combination of the lactobacillus rhamnosus YGG, the bifidobacterium bifidum Y22 and the lactobacillus fermentum FM-12.

As a preferred embodiment of the present invention, said probiotic composition comprises a combination of said lactobacillus reuteri K07, said bifidobacterium bifidum Y22 and said lactobacillus fermentum FM-12.

In a preferred embodiment of the present invention, the probiotic composition comprises a combination of Bifidobacterium bifidum Y22, lactobacillus fermentum FM-12, lactobacillus rhamnosus YGG, and Lactobacillus reuteri K07.

As a preferred embodiment of the present invention, the probiotic composition comprises the Bifidobacterium bifidum Y22, the Lactobacillus fermentum FM-12, the Lactobacillus rhamnosus YGG, and the Lactobacillus reuteri K07 in a weight ratio of 1 (0.125-8): 0.125-8 (0.125-8). The proportion of the four strains in the range can play a role in synergy, promote hair growth, fix hair, prevent alopecia, improve hair strength and increase hair elasticity.

As a preferred embodiment of the present invention, the probiotic composition comprises the Bifidobacterium bifidum Y22, the Lactobacillus fermentum FM-12, the Lactobacillus rhamnosus YGG, and the Lactobacillus reuteri K07 in a weight ratio of 1 (0.125-1.5): (0.5-2): (0.125-1.75). The proportion of the four strains is within the range, and the synergistic effect can be better exerted.

As a preferred embodiment of the present invention, the probiotic composition comprises the bifidobacterium bifidum Y22, the lactobacillus fermentum FM-12, the lactobacillus rhamnosus YGG, and the lactobacillus reuteri K07 in a weight ratio of 1. The four strains are mixed in equal proportion, and the synergistic effect is most obvious.

As a preferred aspect of the present invention, the active ingredients of the probiotic composition comprise a first active ingredient and a second active ingredient; the first active ingredient is provided by the oil-controlling strain of the invention and the second active ingredient is provided by the anti-inflammatory strain of the invention.

As a preferred embodiment of the present invention, the first active ingredient comprises one or any combination of live cells, inactivated cells, fermentation product and lysate of bifidobacterium bifidum Y22; and/or one or any combination of living bacteria, inactivated bacteria, fermentation product and lysate of the lactobacillus fermentum FM-12.

In a preferred embodiment of the present invention, the second active ingredient comprises one or any combination of live bacteria, inactivated bacteria, fermentation product and lysate of lactobacillus rhamnosus YGG; and/or one or any combination of living thallus, inactivated thallus, fermentation product and lysate of the lactobacillus reuteri K07.

As a preferred embodiment of the present invention, the probiotic composition is capable of improving hair health, comprising: promoting hair growth, fixing hair, preventing alopecia, improving hair strength, and increasing hair elasticity.

The second purpose of the invention is realized by adopting the following technical scheme:

the invention also provides the use of the probiotic composition according to any one of the objects for the preparation of a product for improving hair health, wherein the improvement of hair health comprises the promotion of hair growth, hair strengthening, hair loss prevention, hair strength improvement and hair elasticity improvement.

As a preferred embodiment of the present invention, the hair health improving product is an oral preparation; the dosage forms of the oral preparation comprise oral liquid, drops, tablets, powder, capsules and granules.

As a preferred embodiment of the present invention, the hair health improving product is an external preparation; the dosage forms of the external preparation comprise liquid preparations, emulsifying agents, ointments and sticking agents.

As a preferred embodiment of the present invention, the hair health-improving products include pharmaceuticals, functional foods and cosmetics.

As a preferred embodiment of the invention, the invention provides a probiotic freeze-dried powder for improving the health condition of hair, which comprises the probiotic composition in any one of the purposes. The probiotic freeze-dried powder can be orally taken.

In a preferred embodiment, the invention provides a probiotic lysate for improving hair health, which is obtained by lysing a probiotic composition according to any one of the objects. The probiotic lysate may be administered orally or topically.

The probiotic lysate for improving the health condition of hair provided by the invention is formed by lysing the probiotic composition, does not mean that the final product only comprises the lysed probiotic composition, and actually, the final product can also comprise other components according to the actual application condition. For example, probiotic fermentation broths are also included, mixed with the probiotic fermentation broths after lysis. Alternatively, the probiotic fermentation broth is subjected to lysis treatment.

As a preferred embodiment of the present invention, the process for preparing the probiotic lysate comprises: fermenting the probiotic composition according to any one of the objects and then lysing the fermented probiotic composition; or, the probiotic composition of any one of the objects is prepared into probiotic powder, purified water is added for suspension, and finally the lysis treatment is carried out.

As a preferred embodiment of the invention, for the probiotic lysate, lysis methods include, but are not limited to, high pressure homogeneous lysis, sonication lysis and enzymatic lysis.

As a preferred scheme of the invention, the traditional Chinese medicine probiotic fermented product for improving the health condition of hair is prepared by fermenting a traditional Chinese medicine liquid with the probiotic composition according to any one of the purposes, wherein the traditional Chinese medicine liquid is prepared by extracting or pulping the traditional Chinese medicine composition, and the traditional Chinese medicine composition is prepared from the following raw materials: 10-30 parts of black sesame, 5-25 parts of walnut kernel, 2-25 parts of black bean, 5-25 parts of black rice, 5-20 parts of mulberry, 5-20 parts of medlar and 5-20 parts of yam.

The traditional Chinese medicine probiotic fermented product for improving the health condition of hair provided by the invention comprises a traditional Chinese medicine composition comprising black sesame, walnut kernels, black beans, black rice, mulberry, medlar and Chinese yam, and the traditional Chinese medicine composition is extracted or pulped, and then added with the probiotic composition for fermentation to obtain a fermented product containing traditional Chinese medicines and live probiotics.

Compared with the prior art, the invention has the beneficial effects that:

(1) The probiotic composition for improving the health condition of the hair has good effects of promoting hair growth, fixing the hair, preventing alopecia, improving the strength of the hair and increasing the elasticity of the hair.

(2) The probiotic composition for improving the health condition of the hair can be applied to preparing products with various effects, including probiotic freeze-dried powder, probiotic lysate, probiotic traditional Chinese medicine fermentation product and the like; the product can be an oral preparation or an external preparation, and has wide application prospect.

Biological material preservation information:

the strain provided by the invention, bifidobacterium bifidum Y22, with the preservation number of CGMCC NO.15708, is classified and named as: bifidobacterium bifidum has been deposited in 28.4.2018 in China general microbiological culture Collection center (address: ministry No.1 Xilu, north Chen of the south Kogyo, beijing, institute of microbiology, national academy of sciences, postal code: 100101), and the abbreviation of the unit of deposition is CGMCC.

Lactobacillus rhamnosus YGG, the collection number is CGMCC NO.15027, the classification name is: lactobacillus rhamnosus, which has been deposited in the general microbiological culture Collection center of China Committee for culture Collection of microorganisms (address: no. 3, institute of microbiology, national academy of sciences, japan) on 12.7.2017, and the abbreviation of the unit of deposition is CGMCC.

Lactobacillus reuteri K07 with the preservation number of CGMCC NO.15703, is classified and named as: lactobacillus reuteri has been deposited in China general microbiological culture Collection center (address: no. 3 of Xilu No.1 of Beijing, chaoyang district, ministry of microbiology, china academy of sciences, postal code: 100101) in 28.4.2018, and the abbreviation of the collection unit is CGMCC.

Lactobacillus fermentum FM-12 with the deposit number GDMCC NO.62045, with classification and designation: lactobacillus fermentum has been deposited at the Guangdong minimum microbial cultures Collection (address: no. 59 building 5 of Middleya 100, md., guangzhou, ministry of sciences and microbiology, guangdong, inc., postal code: 510070) at 11/8.2021, and the short name of the depository is GDMCC.

Detailed Description

The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment. The raw materials, equipments and the like used in the following examples are commercially available unless otherwise specified.

In the examples of the present invention, the probiotic strains used were all from Guangdong Yivikov technologies, inc.

Test system: the mouse assay system was set up as follows.

In the following experiments of the embodiments of the present invention, the experiments of mice were used to verify the hair growth promotion, hair fixing and hair loss prevention, and the animal model was a sodium sulfide depilation mouse model.

Animal sources: selecting Kunming white mice, which are from southern medical university animal experiment center, have the body weight of 18-22g and are male, and the age is 6-8 weeks.

Establishing a model: taking a mouse, breeding the mouse for 1 week, adapting to the environment, marking a depilation region on the back of the mouse by picric acid, shaving the hair of the mouse marking region by a shaver, and then removing the hair by 6% Na ₂ And (S) carrying out depilation by using the solution. The depilatory area is 10% of the surface area of the mouse body (the depilatory area is about 6 cm calculated according to the Meeh-Rubner formula) ² Thus, it was determined that each mouse was 6 cm ² Unhairing), selecting smooth and non-traumatic mouse as model success model.

Grouping and trial method: the mice successfully modeled are randomly grouped into 8 mice each group, and the mice are divided into a blank control group, a positive control group and a probiotic group. All groups of animals were dosed 1/day for 30 consecutive days.

Blank control group (no drug, jar stomach distilled water).

Positive control group (finasteride, gavage 0.15 mg/kg). When the finasteride is used for treating alopecia, the application method comprises the following steps: orally taking for 1 mg/time and 1 time/day, wherein the dosage of the mouse is 10 times of that of the human body according to the average weight of 70 kilograms of the human body, the gastric lavage amount of the mouse is 0.143 mg/kilogram, and the whole is taken according to 0.15 mg/kilogram.

Probiotic group: mice were gavaged with probiotic powder at 22 hundred million CFU/kg.

Index measurement: the measurement indexes include the length of the new hair, the weight of the new hair and the number of hair follicles.

(1) Measurement of the Length of newborn Hair in mice

At the end of the experiment, 10 new hairs were randomly picked up in the experimental area of each mouse using tweezers and a magnifying glass, the length of the 10 new hairs was measured with a vernier caliper, the average value of the new hair length of each mouse was calculated, and finally the average value of each group was calculated and counted as X.

(2) Mouse new hair weight determination

All new hairs were carefully shaved with a shaver on the last day of dosing, weighed and the mean new hair weight (including the 10 hair weight measured above) was calculated for each group of mice and calculated as Y.

(3) Determination of number of hair follicles in mice

At the end of the experiment, all mice were sacrificed, the skin of the back experimental area where hairs had been shaved was taken, the skin was fixed with 10% formalin, the hair follicle cross section was taken, the number of hair follicles was observed under a microscope, 3 fields were randomly selected for each under x 100 conditions, the number of hair follicles was counted, the average of the 3 fields was taken as a sample hair follicle count, and was counted as Z.

When scoring the test sample, the above-mentioned 3 criteria were considered in combination. The embodiment of the invention adopts a standard score calculation method, and the specific calculation process is as follows: average hair length group (X) _i ) Each group of data was compared to a blank control group (X) ₀ ) By a difference of (X) _i -X ₀ ) The maximum difference is 100 points, the blank control group is 0 points (the blank group is also counted as an index group, namely the blank control group-blank control group, and 0 is obtained, the counting standard is 0 point, if the data of the sample group is smaller than that of the blank group, the sample has the reverse phase effect and is directly shaved off). The other groups were scored as: (X) _i -X ₀ ）/（X _i -X ₀ ） _Max *100. The average hair weight Y and the average hair follicle number Z are calculated according to the same method.

Among the factors, hair follicles are a key factor for hair growth, and healthy hair can grow only in mature hair follicles, so that the weight is given to 40%, and the hair length index and the hair weight index are given to 30% respectively. The final scores for each group were calculated according to the following formula: each set of scores = (X) _i -X ₀ ）/（X _i -X ₀ ） _Max *100*30%+（Y _i -Y ₀ ）/（Y _i -Y ₀ ） _Max *100*30%+（Z _i -Z ₀ ）/（Z _i -Z ₀ ） _Max *100*40%。

Verification system one: screening test for probiotics to promote hair growth, fix hair and prevent alopecia

1. Preliminary screening test for probiotics

A series of probiotic strains are subjected to a mouse hair-fixing and hair-loss-preventing test, and comprise bifidobacterium adolescentis Y54, bifidobacterium bifidum Y22, lactobacillus acidophilus K43, lactobacillus paracasei K9, bifidobacterium animalis subsp lactis Y6, bifidobacterium longum W68, lactobacillus reuteri K07, pediococcus pentosaceus LN-PT16, lactobacillus rhamnosus YGG and lactobacillus fermentum FM-12. And (3) taking freeze-dried powder of the strain, performing intragastric administration on the mouse according to the test system, testing, measuring the 3 groups of indexes after the test is finished, and calculating the standard score. The specific results are shown in Table 1 below.

Mouse dose conversion method: for a probiotic experiment group, the probiotic is eaten per person in an amount of 150 hundred million CFU (circulating fluid units) per day, the weight of a human body is 70 kilograms on average, the dosage per kilogram of the human body is 2.14 hundred million/kg, the dosage of a white mouse is 10 times that of the human body, namely 21.4 hundred million CFU/kg, and the whole is taken in an amount of 22 hundred million CFU/kg (counted upwards) to be irrigated to the stomach of the mouse (in order to facilitate the operation of irrigating the stomach, 1ml of purified water is added for suspension during the irrigation of the stomach).

The positive control group (finasteride, gavage 0.15 mg/kg) has a gavage volume of 1ml.

The blank group was subjected to intragastric administration with distilled water, and the intragastric administration volume was 1ml.

TABLE 1 test results of different strains

From the results of the standard components in table 1 above, the standard component values of a single strain are, from large to small: bifidobacterium bifidum Y22, lactobacillus rhamnosus YGG, lactobacillus reuteri K07, lactobacillus fermentum FM-12, lactobacillus acidophilus K43 group, bifidobacterium animalis subspecies lactis Y6 group, pediococcus pentosaceus LN-PT16 group, bifidobacterium longum W68 group, lactobacillus paracasei K9 group, and Bifidobacterium adolescentis Y54 group.

Wherein the standard score of Bifidobacterium bifidum Y22, lactobacillus rhamnosus YGG, lactobacillus reuteri K07 and Lactobacillus fermentum FM-12 is higher and is separated from other groups, which indicates that the above 4 strains have better hair growth promoting effect.

The function of each single strain is analyzed, wherein bifidobacterium bifidum Y22 and lactobacillus fermentum FM-12 have better oil control effect. Lactobacillus rhamnosus YGG and Lactobacillus reuteri K07 have good anti-inflammatory effect. While both oil control and anti-inflammatory have positive effects on hair growth. Of course, the 4 strains also have better gastrointestinal tract conditioning function.

2. Probiotic combination screening assay

4 strains screened out are compounded to verify whether better effects can be obtained. The results are shown in table 2 below.

TABLE 2 recording table of the results of the combined tests of strains

Remarking: the above proportions are counted by the total number of colonies, and are proportioned in equal proportion. The standard score calculation method is the same as that in Table 1.

The results in table 2 above show that the use of oil-controlling bacteria in combination with anti-inflammatory bacteria results in better hair growth than a combination of strains with only a single effect. The 2 strains in the 3 rd group are oil control bacteria, the 2 strains in the 4 th group are anti-inflammatory bacteria, the scores of the bacteria are not much different from those of single strains and are all lower than those of the groups 5-9, and basically no synergistic effect exists.

And the results of groups 5-9 show that the average hair length X (mm), the average hair weight Y (mg) and the average hair follicle number Z (number) are better than the single-strain groups of bifidobacterium bifidum Y22, lactobacillus rhamnosus YGG, lactobacillus reuteri K07 and lactobacillus fermentum FM-12 in the table 1, which indicates that the efficacy is better than that of the single strain by adopting oil-controlling bacteria and anti-inflammatory bacteria for matching. Particularly, in the group 7, the standard score obtained by compounding 4 strains of bacteria is the highest, which shows that the 4 strains of bacteria have the best matching effect.

3. Probiotic combination ratio screening test

4 strains of the screened bacteria are compounded according to different proportions, and the effects of promoting hair growth, fixing hair and preventing alopecia of the strain combination under different proportions are verified. The ratios of the 4 strains and the test results are shown in Table 3 below.

TABLE 3 Strain proportioning Table

Remarking: the above proportions are counted and proportioned according to the total number of colonies.

The composite strain freeze-dried powder of the combinations 1 to 9 is taken, and the gavage test is carried out on the mice according to the test system, and the test results are shown in the following table 4.

TABLE 4 test results of different ratios of strains

From the test data results in table 4 above, the test results of hair-fixing and anti-hair loss are as follows: the compound strain combination is 9, the compound strain combination is 7, the compound strain combination is 8, the compound strain combination is 1, the compound strain combination is 2, the compound strain combination is 3, the compound strain combination is 4, the compound strain combination is 5, and the compound strain combination is 6.

By combining the results of Table 1, the compound strain can better promote hair growth, fix hair and prevent alopecia. And when the 4 strains are uniformly matched, the effect is better. When the proportion of one strain in the formula is smaller, the score value is reduced. Particularly, when the proportion of strains with the same functions is small, the score value is reduced more obviously.

The results in table 4 show that different strains, especially different functional strains, can exert a synergistic effect. In the combination 1-9, the weight ratio of the bifidobacterium bifidum Y22 to the lactobacillus fermentum FM-12 to the lactobacillus rhamnosus YGG to the lactobacillus reuteri K07 is 1 (0.125-8) to (0.125-8). Particularly, the effect is better when the weight ratio of the bifidobacterium bifidum Y22 to the lactobacillus fermentum FM-12 to the lactobacillus rhamnosus YGG to the lactobacillus reuteri K07 is 1 (0.125-1.5) to (0.5-2) to (0.125-1.75) (the combination is 1-2 and 7-9). And bifidobacterium bifidum Y22: lactobacillus rhamnosus YGG: lactobacillus reuteri K07: lactobacillus fermentum FM-12=2.5:2.5:2.5:2.5, namely 4 strains, the effect is best.

And (2) verifying a system II: application extension

According to the functions and research of the traditional Chinese medicines, part of the traditional Chinese medicines also have good effects of promoting hair growth, fixing hair, preventing alopecia and blackening hair. Selecting a traditional Chinese medicine composition with better nutrients required by fermentation, adding glucose as a nutrient substrate, performing combined fermentation by using the above 4 strains of bacteria, filtering fermentation liquor (rough filtering, retaining viable bacteria), freeze-drying to obtain the probiotic fermented traditional Chinese medicine composition, and verifying the effect of promoting hair growth.

Meanwhile, in order to verify the effect of the 4 strains of bacteria in external application, two probiotic samples are prepared for verification, and the sample 1 is probiotic freeze-dried powder added with purified water to obtain probiotic suspension. Sample 2 is a lysate obtained by lysing sample 1.

Specific embodiments are as follows.

Example 1

A probiotic fermented traditional Chinese medicine product for fixing hair and preventing alopecia comprises the following probiotic fermented traditional Chinese medicine processes:

(1) Mixing 20 parts of black sesame, 15 parts of walnut kernels, 15 parts of black beans, 15 parts of black rice, 10 parts of mulberries, 10 parts of medlar and 10 parts of Chinese yams, adding 10 times of purified water for soaking, and homogenizing to obtain serous fluid.

(2) Transferring the slurry to a sterilized fermenter, adding 2.0% glucose as nutrient substrate based on the weight of the purified water added in step (1), stirring to dissolve, adjusting pH to 6.0, sterilizing, and cooling to 37 deg.C.

(3) Respectively activating and culturing Bifidobacterium bifidum Y22, lactobacillus rhamnosus YGG, lactobacillus reuteri K07 and Lactobacillus fermentum FM-12 seed glycerol tubes with MRS liquid culture medium to obtain first-stage seed solution. Then adding MRS liquid culture medium into the first seed liquid to amplify to obtain second seed liquid.

(4) Culturing: adding the mixed secondary seed liquid into a sterilization culture tank according to the proportion of 4 percent (based on the weight of the total added water), and carrying out anaerobic culture at 37 ℃ for 12 hours to obtain the probiotic traditional Chinese medicine fermentation liquid.

(5) Discharging culture solution, primarily filtering with 100 mesh, removing residue, and filtering with 400 mesh filter cloth to obtain probiotic fermented extract. Meanwhile, the fermented extract is taken for bacterial load detection, 2 parallel samples are taken for measurement, and the bacterial load result is 9.1 hundred million CFU/ml.

(6) Further concentrating the probiotic fermented extract of the traditional Chinese medicine at low temperature (lower than 40 ℃) until the medicinal materials: and (3) fermenting the concentrated solution (1), adding trehalose with the liquid amount of 20% as a freeze-drying protective agent, placing the freeze-drying protective agent in a freeze dryer, and freeze-drying according to the following procedures to obtain the traditional Chinese medicine probiotic fermented extract freeze-dried powder. The lyophilization procedure was as follows: pre-freezing: freezing at-42 ℃ to-50 ℃ for 4h, and then performing vacuum drying under the drying conditions: the vacuum degree is 2 to 5Pa, the drying temperature is-40 to 26 ℃, and the drying time is 40 to 50h. And (3) judging a drying end point: the vacuum degree of the drying chamber does not rise to 15Pa after 10 minutes without vacuumizing. Taking out, and fermenting to obtain probiotic fermented Chinese medicinal lyophilized powder.

Example 2

Example 2 differs from example 1 in that: probiotics were not inoculated and the procedure was the same throughout the rest of the procedure. Comparing the effect of probiotic fermentation with that of probiotic fermentation without probiotic.

Example 3

A probiotic lysate for fixing hair and preventing alopecia is prepared by the following steps:

(1) And (3) mixing bifidobacterium bifidum Y22, lactobacillus rhamnosus YGG, lactobacillus reuteri K07 and lactobacillus fermentum FM-12 powder according to the equal ratio of the total number of the colonies of the probiotics to obtain the composite bacteria powder.

(2) And (3) adding purified water into the compound bacteria powder to obtain a compound probiotic suspension with the concentration of 4% (mass ratio).

Example 4

Example 4 differs from example 3 in that: taking the composite probiotic suspension prepared in the example 3, and homogenizing the suspension by using a high-pressure homogenizer under the conditions: the pressure is 1200bar, and the composite probiotic lysate is obtained after 2 times (suspension type, milk-shaped, and sealed preservation at the low temperature of 2-6 ℃).

Example 5

Example 5 differs from example 4 in that: taking the composite probiotic lysate of the example 4, adding composite protease (papain, neutral protease and alkaline protease), carrying out enzymolysis for 5 hours at 50 ℃, heating to 90 ℃ to inactivate the enzyme, cooling to 50 ℃, centrifuging, and taking supernatant fluid to obtain the composite probiotic lysate (clear type, normal temperature closed storage).

The samples of examples 1 to 5 were sampled and tested for their effects according to the above-mentioned "test system".

Groups 3 to 7: samples from examples 1-5, respectively. Example 2 mice were gavaged with the same amount of traditional Chinese medicine as in example 1 without probiotics. Example 3 is a complex probiotic suspension (topical), example 4 is a complex probiotic lysate (suspension type), and example 5 is a complex probiotic lysate (clarified type). When the samples are externally used, the concentration is 0.02ml/cm ² Is uniformly applied to the depilatory part of the mouse.

Group 8: mixing a composite probiotic group, namely bifidobacterium bifidum Y22, lactobacillus rhamnosus YGG, lactobacillus reuteri K07 and lactobacillus fermentum FM-12 according to the equal ratio of the total number of colonies to form a composite probiotic group; mice were gavaged with 22 hundred million CFU/kg probiotic.

Mouse dose conversion method: according to the daily administration of 150 hundred million CFU probiotic-containing traditional Chinese medicine fermentation liquid for each person, the weight of a human body is calculated according to average 70 kilograms, the administration amount of each kilogram of the body is 2.14 hundred million/kg, the dosage of a white mouse is 10 times of that of the human body, namely 21.4 hundred million/kg, the fermentation liquid is calculated according to 9.1 hundred million CFU/ml, namely, the stomach filling amount of the mouse is 2.35 ml/kg, and the whole mouse is filled with the stomach according to 2.5 ml/kg (the blank group is filled with purified water for stomach filling). The specific results are shown in Table 5 below.

TABLE 5 mouse test results of applied samples are recorded in Table

Remarking: the standard score calculation method is the same as that in table 1.

As can be seen from table 5 above:

(1) Group 6, example 4 (suspension) and group 7, example 5 (clear), the composite probiotic lysate had a hair growth promoting effect, and the clear form of group 7 was slightly superior to the suspension form of group 6.

(2) Group 3 the group 1 of the example had a comprehensive score higher than that of the group 4 of the example 2 and also higher than that of the group 8 of the composite probiotics group, which indicates that the effect of promoting hair growth by the combined action of the probiotic fermented traditional Chinese medicines is better than that of the single traditional Chinese medicine or the single composite probiotics.

(3) The combined score of the group 9 composite probiotic + example 5 (clarified) group was higher than that of the group 8 composite probiotic group and that of the group 7 example 5 (clarified) group, indicating that the oral composite probiotic and the topical composite probiotic lysate had a synergistic effect on hair growth. The group 5 composite probiotic suspension is externally applied, the score value is very low and is less than 10, the result difference with a blank control group is very small, and the result shows that the hair growth promotion effect is almost absent when the pure probiotics are externally applied.

The research results show that the probiotics oral administration, the probiotics fermented traditional Chinese medicine product oral administration and the probiotics lysate external coating have the hair growth promoting effect, and compared with the oral administration of distilled water, the hair growth speed is higher, the weight of newly grown hair is heavier, and the number of newly grown hair follicles is more.

The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention should not be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are intended to be covered by the claims.

Claims

1. Use of a probiotic composition in the manufacture of a product for improving the health of hair, wherein the probiotic composition comprises one or a combination of bifidobacterium bifidum and lactobacillus fermentum; and, one or a combination of two of lactobacillus rhamnosus and lactobacillus reuteri; the bifidobacterium bifidum is bifidobacterium bifidum Y22 with the preservation number of CGMCC NO. 15708; the lactobacillus fermentum is lactobacillus fermentum FM-12 with the preservation number of GDMCC NO. 62045; the lactobacillus rhamnosus is Lactobacillus rhamnosus YGG with the preservation number of CGMCC NO. 15027; the lactobacillus reuteri is lactobacillus reuteri K07 with the preservation number of CGMCC NO. 15703.

2. The use of claim 1, wherein the probiotic composition comprises a combination of the bifidobacterium bifidum Y22 and the lactobacillus rhamnosus YGG;

or, a combination comprising said bifidobacterium bifidum Y22 and said lactobacillus reuteri K07;

or, a combination comprising the lactobacillus fermentum FM-12 and the lactobacillus rhamnosus YGG;

or, a combination comprising said lactobacillus fermentum FM-12 and said lactobacillus reuteri K07;

or, a combination comprising said bifidobacterium bifidum Y22, said lactobacillus rhamnosus YGG and said lactobacillus reuteri K07;

or, a combination comprising the lactobacillus fermentum FM-12, the lactobacillus rhamnosus YGG, and the lactobacillus reuteri K07;

or, a combination comprising the Lactobacillus rhamnosus YGG, the Bifidobacterium bifidum Y22 and the Lactobacillus fermentum FM-12;

or, a combination comprising said lactobacillus reuteri K07, said bifidobacterium bifidum Y22 and said lactobacillus fermentum FM-12;

or a combination of said Bifidobacterium bifidum Y22, said Lactobacillus fermentum FM-12, said Lactobacillus rhamnosus YGG, and said Lactobacillus reuteri K07.

3. The use of claim 1, wherein the probiotic composition comprises the bifidobacterium bifidum Y22, the lactobacillus fermentum FM-12, the lactobacillus rhamnosus YGG, and the lactobacillus reuteri K07 in a weight ratio of 1 (0.125-8): (0.125-8).

4. The use of claim 1, wherein the active ingredients of the probiotic composition comprise a first active ingredient and a second active ingredient;

the first active component comprises one or any combination of living bacteria, inactivated bacteria, fermentation product and lysate of the bifidobacterium bifidum Y22; and/or, one or any combination of living bacteria, inactivated bacteria, fermentation product and lysate of the lactobacillus fermentum FM-12;

the second active component comprises one or any combination of live bacteria, inactivated bacteria, fermentation product and lysate of the Lactobacillus rhamnosus YGG; and/or, one or any combination of live thallus, inactivated thallus, fermentation product and lysate of the lactobacillus reuteri K07.

5. The use of claim 1, wherein the improvement in hair health comprises: promoting hair growth, fixing hair, preventing alopecia, improving hair strength, and increasing hair elasticity.

6. The use according to claim 1, wherein the hair health improving product is an oral preparation; the dosage forms of the oral preparation comprise oral liquid, drops, tablets, powder, capsules and granules.

7. A probiotic composition for improving the health of hair, characterized in that it comprises a combination of bifidobacterium bifidum and lactobacillus rhamnosus;

or, a combination comprising lactobacillus fermentum and lactobacillus rhamnosus;

or, a combination comprising lactobacillus fermentum and lactobacillus reuteri;

or, a combination comprising bifidobacterium bifidum, lactobacillus rhamnosus and lactobacillus reuteri;

or, a combination comprising lactobacillus fermentum, lactobacillus rhamnosus, and lactobacillus reuteri;

or, a combination comprising lactobacillus rhamnosus, bifidobacterium bifidum, and lactobacillus fermentum;

or, a combination comprising lactobacillus reuteri, bifidobacterium bifidum, and lactobacillus fermentum;

or, a combination comprising bifidobacterium bifidum, lactobacillus fermentum, lactobacillus rhamnosus, and lactobacillus reuteri;

the bifidobacterium bifidum is bifidobacterium bifidum Y22 with the preservation number of CGMCC NO. 15708; the lactobacillus fermentum is lactobacillus fermentum FM-12 with the preservation number of GDMCC NO. 62045; the lactobacillus rhamnosus is Lactobacillus rhamnosus YGG with the preservation number of CGMCC NO. 15027; the lactobacillus reuteri is lactobacillus reuteri K07 with the preservation number of CGMCC NO. 15703.

8. The probiotic composition for improving the health condition of hair according to claim 7, comprising the Bifidobacterium bifidum Y22, the Lactobacillus fermentum FM-12, the Lactobacillus rhamnosus YGG, and the Lactobacillus reuteri K07 in a weight ratio of 1 (0.125-8): (0.125-8): (0.125-8).

9. The probiotic composition for improving hair health of claim 8, comprising the Bifidobacterium bifidum Y22, the Lactobacillus fermentum FM-12, the Lactobacillus rhamnosus YGG, and the Lactobacillus reuteri K07 in a weight ratio of 1 (0.125-1.5): (0.5-2): (0.125-1.75).

10. The probiotic composition for improving the health condition of hair according to claim 8, characterized in that it comprises the bifidobacterium bifidum Y22, the lactobacillus fermentum FM-12, the lactobacillus rhamnosus YGG and the lactobacillus reuteri K07 in a weight ratio of 1.

11. A probiotic freeze-dried powder for improving the health condition of hair, characterized in that it comprises a probiotic composition according to any one of claims 7 to 10.

12. A probiotic lysate for improving the health of hair, prepared by lysing the probiotic composition of any one of claims 7-10.

13. A traditional Chinese medicine probiotic fermented product for improving the health condition of hair is characterized in that the traditional Chinese medicine liquid is prepared by fermenting the probiotic composition according to any one of claims 7 to 10, the traditional Chinese medicine liquid is prepared by extracting or pulping the traditional Chinese medicine composition, and the traditional Chinese medicine composition is composed of the following raw materials: 10-30 parts of black sesame, 5-25 parts of walnut kernels, 2-25 parts of black beans, 5-25 parts of black rice, 5-20 parts of mulberries, 5-20 parts of medlar and 5-20 parts of Chinese yams.