CN115624116A - Compound feed for improving oxidation resistance and disease resistance of carps - Google Patents
Compound feed for improving oxidation resistance and disease resistance of carps Download PDFInfo
- Publication number
- CN115624116A CN115624116A CN202211414027.6A CN202211414027A CN115624116A CN 115624116 A CN115624116 A CN 115624116A CN 202211414027 A CN202211414027 A CN 202211414027A CN 115624116 A CN115624116 A CN 115624116A
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- China
- Prior art keywords
- parts
- meal
- coenzyme
- carps
- compound feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Images
Classifications
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
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Abstract
The invention discloses a compound feed for improving oxidation resistance and disease resistance of carps, which is prepared from the following materials in parts by weight: 3-8 parts of fish meal, 7-10 parts of chicken powder, 25-32 parts of soybean meal, 9-12 parts of rapeseed meal, 6-10 parts of cottonseed meal, 7-12 parts of flour, 7-10 parts of wheat middling, 7-10 parts of bran, 3-5 parts of soybean oil, 0.5-1 part of multi-vitamin multi-mineral premix, 0.1-0.5 part of choline, 1-3 parts of sodium carboxymethyl cellulose, 5-9 parts of bentonite, 1-2.5 parts of monocalcium phosphate, 0.1-0.3 part of methionine, 0.1-0.5 part of lysine and 0.012-0.02 part of coenzyme Q. The compound feed provided by the invention can enhance the disease resistance of carps, improve the growth performance of the carps and the utilization rate of the feed, and has a good application prospect.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to a compound feed for improving the oxidation resistance and disease resistance of carps.
Background
Carp, named as carp seed and carp raisin, is a omnivorous freshwater fish which is rich in high-quality protein, has the amino acid variety of more than ten, comprises eight essential amino acids for human body, has the human body digestibility of 96 percent, and can relieve arteriosclerosis and coronary heart disease. The carp has low fat content, proper calcium-phosphorus ratio and low price, and can meet the requirements of consumers.
Carp as one of the most important economic freshwater fishes creates great economic benefit for aquaculture industry in Europe and Asia. The total yield of freshwater fish culture in 2021 years is 2640 ten thousand tons, wherein the total yield of carps is about 283 ten thousand tons. With the rapid development of aquaculture industry, intensive culture mode is adopted for carp culture. However, intensive cultivation methods bring high economic benefits, reduce the growth performance of the cultivated fishes, cause economic losses, increase the risk of infectious diseases, and lead to mass death of the cultivated fishes, thereby limiting the development of aquaculture industry.
To avoid this, many farms abuse antibiotics for disease control and prevention. However, abuse of antibiotics can lead to the development of resistant strains that are detrimental to ecosystem and human health. For the sustainable development of aquaculture industry, an additive which can not only avoid the harm to the aquaculture water area, but also promote the health of the cultured fishes and improve the growth performance is needed. Researches find that the disease resistance and the immune response can be enhanced by adding the antioxidant into the aquatic feed, the aquaculture yield is improved, and the sustainable development is realized.
Coenzyme Q10 is the only endogenously synthesized lipid-soluble antioxidant that occurs naturally in the biological system of the inner mitochondrial membrane and plays a crucial role in the Electron Transport Chain (ETC) and energy synthesis (ATP). Coenzyme Q10 is mainly present in humans, birds and fish and plays an important role in cellular metabolism and oxidative balance. Coenzyme Q10 has both oxidized and reduced forms, and only the reduced form has antioxidant effect, and can scavenge free radicals, protect DNA, cell membrane, lipid and protein from risk of oxidative damage, help vitamin E regeneration, and maintain healthy energy level. Coenzyme Q10 is not naturally formed in animals in sufficient quantities and needs to be provided from an external source. Therefore, exogenous supplementation of this super vitamin in fish feed is suggested. In addition, the solubility of coenzyme Q10 in lipid and organic solvents and the water insolubility make the coenzyme Q10 an ideal additive for aquatic animal feed.
Disclosure of Invention
The invention mainly aims to provide the compound feed which can improve the oxidation resistance and immunity of carps, enhance the disease resistance and improve the growth performance and the feed utilization rate.
In order to achieve the purpose, the invention adopts the following technical scheme:
a compound feed for improving oxidation resistance and disease resistance of carps is prepared from the following materials in parts by weight: 3-8 parts of fish meal, 7-10 parts of chicken powder, 25-32 parts of soybean meal, 9-12 parts of rapeseed meal, 6-10 parts of cottonseed meal, 7-12 parts of flour, 7-10 parts of wheat middling, 7-10 parts of bran, 3-5 parts of soybean oil, 0.5-1 part of multi-vitamin multi-mineral premix, 0.1-0.5 part of choline, 1-3 parts of sodium carboxymethylcellulose, 5-9 parts of bentonite, 1-2.5 parts of monocalcium phosphate, 0.1-0.3 part of methionine, 0.1-0.5 part of lysine and 0.012-0.02 part of coenzyme Q.
Preferably, the compound feed is prepared from the following materials in parts by weight: 5 parts of fish meal, 8 parts of chicken powder, 28 parts of soybean meal, 11 parts of rapeseed meal, 8 parts of cottonseed meal, 8 parts of flour, 9 parts of wheat middling, 9 parts of bran, 3 parts of soybean oil, 1 part of multi-vitamin multi-mineral premix, 0.2 part of choline, 1 part of sodium carboxymethylcellulose, 5.338 parts of bentonite, 2 parts of monocalcium phosphate, 0.15 part of methionine, 0.25 part of lysine and 0.012 part of coenzyme Q.
The preparation method of the compound feed comprises the steps of respectively crushing fish meal, chicken meal, bean pulp, rapeseed meal, cottonseed meal, flour, wheat middling, bran, soybean oil, multi-vitamin multi-mineral premix, choline, sodium carboxymethylcellulose, bentonite, monocalcium phosphate, methionine, lysine and coenzyme Q10, sieving with a 40-mesh sieve, weighing according to a proportion, fully stirring and uniformly mixing with the soybean oil by adopting a step-by-step amplification method, extruding into long strips with the thickness of 1.5-5 mm by using a pellet feed granulator, cutting into pellets with the length of 3-10 mm, drying at 50 ℃ for 18 hours, and refrigerating.
Compared with the existing natural bait, the carp compound feed has the following outstanding effects:
1. the compound feed for the carps has good performance and strong food calling performance, can effectively promote the growth and the feed utilization of the carps, improves the digestibility, and improves the survival rate of the carps, thereby improving the economic benefit of breeding.
2. The invention has scientific and reasonable process, simplicity, convenience and feasibility and lower processing cost.
3. The compound feed does not contain any antibiotic and chemical hormone, has the functions of better improving the immunity and the antioxidant capacity of the carps by adding the coenzyme Q10, provides enough nutritional energy for the carps by compounding the plant protein and the animal protein, and has good shape-preserving effect by adding the sodium carboxymethyl cellulose as the adhesive.
Drawings
FIG. 1 is a graph showing the effect of coenzyme Q10 on the cumulative survival rate of juvenile carps after challenge with Aeromonas hydrophila in example 2.
Detailed Description
A compound feed for improving oxidation resistance and disease resistance of carps is prepared by respectively crushing fish meal, chicken meal, bean pulp, rapeseed meal, cottonseed meal, flour, wheat middling, bran, multi-vitamin multi-mineral premix, choline, sodium carboxymethylcellulose, bentonite, monocalcium phosphate, methionine, lysine and coenzyme Q10, sieving with a 40-mesh sieve, then fully extruding into long-strip feed granules with the length of 3-10 mm after 3-8 parts by weight of fish meal, 7-10 parts by weight of chicken meal, 25-32 parts by weight of bean pulp, 9-12 parts by weight of rapeseed meal, 6-10 parts by weight of cottonseed meal, 7-12 parts by weight of flour, 7-10 parts by weight of wheat middling, 7-10 parts by weight of bran, 0.5-1 part by weight of multi-mineral premix, 0.1-0.5 part by weight of choline, 1-3 parts by weight of sodium carboxymethylcellulose, 5-9 parts by weight of bentonite, 1-2.5 parts by weight of monocalcium phosphate, 0.1-0.3 part by weight of methionine, 0.1-0.5 part by weight of coenzyme, 0.5 parts by weight of Q10.012, 0.5.5 mm by weight of soybean oil and fully extruding into long-5 mm soybean oil granules with a cold preservation method, and finally extruding into long-grain size granules with the length of 50-5 mm after being dried by a cold preservation method.
Carp is bred for 8 weeks by using feeds prepared by different addition amounts of coenzyme Q10, and the results are shown in table 1.
TABLE 1 influence of different coenzyme Q10 additions on juvenile carp growth, feed utilization and body indices (mean. + -. Standard error)
As can be seen from the table 1, when the coenzyme Q10 is added into the feed in an amount of 120 mg/kg for feeding the carp, the weight of the carp is obviously increased, the feed efficiency is obviously improved, the activities of liver superoxide dismutase (SOD) and glutathione mercaptotransferase (GST) are obviously improved, the content of Malondialdehyde (MDA) is obviously reduced, and the oxidation resistance of the carp is obviously improved.
Inoculating Aeromonas hydrophila on FWA medium, culturing at 30 deg.C for 36 hr, and eluting with 0.85% sterile normal saline to obtain 3.8 × 10 bacterial solution 7 cfu/mL. After 8 weeks of culture test, 0.3 mL of viable aeromonas hydrophila suspension is injected at the base of the pectoral fin for toxicity attack test, the death conditions 1-10 days after toxicity attack are respectively recorded, and the accumulated survival rate is calculated, and the result is shown in figure 1.
The results in figure 1 show that the cumulative survival rate of the carp fed with the feed group added with 120 mg/kg of coenzyme Q10 after being infected by aeromonas hydrophila for 10 days is obviously improved, which indicates that the coenzyme Q10 improves the disease resistance of the carp to the aeromonas hydrophila.
In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.
The multivitamin and multi-mineral premix comprises 60 ten thousand IU/kg of vitamin A, 3 ten thousand IU/kg of vitamin D, 6 g/kg of vitamin E, 0.5 g/kg of vitamin K, 0.6 g/kg of vitamin B, 2 g/kg of vitamin B, 0.7 g/kg of vitamin B, 12 mg/kg of vitamin B, 10 g/kg of vitamin C, 3 g/kg of D-pantothenic acid, 5 g/kg of nicotinamide, 0.4 g/kg of folic acid, 12 mg/kg of D-biotin, 8 g/kg of inositol, 5 g/kg of magnesium, 3 g/kg of zinc, 1.5 g/kg of manganese, 0.4 g/kg of copper, 10 g/kg of iron, 120 mg/kg of cobalt, 150 mg/kg of iodine and 20 mg/kg of selenium.
Example 1
Respectively crushing fish meal, chicken meal, soybean meal, rapeseed meal, cottonseed meal, flour, wheat middling, bran, multi-vitamin multi-mineral premix, choline, sodium carboxymethylcellulose, bentonite, monocalcium phosphate, methionine, lysine and coenzyme Q10, sieving by a 40-mesh sieve, then weighing 5kg of fish meal, 8 kg of chicken meal, 28 kg of soybean meal, 11 kg of rapeseed meal, 8 kg of cottonseed meal, 8 kg of flour, 9 kg of wheat middling, 9 kg of bran, 1 kg of multi-vitamin multi-mineral premix, 0.2 kg of choline, 1 kg of sodium carboxymethylcellulose, 5.338 kg of bentonite, 2 kg of monocalcium phosphate, 0.15 kg of methionine, 0.25 kg of lysine and 0.012 kg of coenzyme Q, uniformly stirring the raw materials by a step-by-step amplification method and 3kg of soybean oil in a feed stirrer, extruding the raw materials into 2 mm-thick strips by a pellet feed granulator, cutting the strips into 4 mm-long particles, drying the particles at 50 ℃ for 18 hours, and storing the particles at 0 ℃ in a refrigeration temperature.
Experiments are carried out in an aquaculture base of the engineering and scientific training center under Fujian agriculture and forestry university, the carp used in the experiments is about 9.8g, the feed obtained in the embodiment is adopted for cultivation, the average size of the carp reaches 82.3g after 12 weeks, and compared with a control group fed with a comparative feed (except that coenzyme Q10 is not added to the comparative feed, other feed components are the same as those of the feed prepared in the embodiment), the weight gain rate of the carp is improved by 6.5%, and the feed efficiency is improved by 6.3%. Meanwhile, compared with a control group, the addition of the coenzyme Q10 into the feed can obviously increase the intestinal villus length and the villus density of the carp, and improve the dry matter digestibility, which indicates that the coenzyme Q10 has a certain improvement effect on the intestinal digestion and absorption capacity of the carp.
Example 2
Respectively crushing fish meal, chicken meal, soybean meal, rapeseed meal, cottonseed meal, flour, wheat middling, bran, multi-vitamin multi-mineral premix, choline, sodium carboxymethylcellulose, bentonite, monocalcium phosphate, methionine, lysine and coenzyme Q10, sieving by a 40-mesh sieve, then weighing 4kg of fish meal, 8 kg of chicken meal, 28 kg of soybean meal, 11 kg of rapeseed meal, 8 kg of cottonseed meal, 8 kg of flour, 7 kg of wheat middling, 8 kg of bran, 1 kg of multi-vitamin multi-mineral premix, 0.2 kg of choline, 1 kg of sodium carboxymethylcellulose, 5.338 kg of bentonite, 2 kg of monocalcium phosphate, 0.15 kg of methionine, 0.25 kg of lysine and 0.012 kg of coenzyme Q, uniformly stirring the raw materials by a step-by-step amplification method and 4kg of soybean oil in a feed stirrer, extruding the raw materials into 3mm thick and thin strips by a pellet feed granulator, cutting the raw materials into 6mm long pellets, and finally drying the pellets at 50 ℃ for 18 hours and storing at 0 ℃ for 18 hours.
When experiments are carried out in a certain breeding base in Fuzhou Minhou, the specification of carps used in the experiments is about 51.2g, the feed obtained in the embodiment is adopted for breeding, the average specification of the carps reaches 120.5g after 8 weeks, and compared with a control group fed with a comparative feed (the comparative feed used in the control group is the same as the feed prepared in the embodiment except that coenzyme Q10 is not added), the weight gain rate is improved by 6.9 percent, and the feed cost is reduced by 7.1 percent.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (3)
1. The compound feed for improving the oxidation resistance and the disease resistance of carps is characterized by being prepared from the following materials in parts by weight: 3-8 parts of fish meal, 7-10 parts of chicken powder, 25-32 parts of soybean meal, 9-12 parts of rapeseed meal, 6-10 parts of cottonseed meal, 7-12 parts of flour, 7-10 parts of wheat middling, 7-10 parts of bran, 3-5 parts of soybean oil, 0.5-1 part of multi-vitamin multi-mineral premix, 0.1-0.5 part of choline, 1-3 parts of sodium carboxymethyl cellulose, 5-9 parts of bentonite, 1-2.5 parts of monocalcium phosphate, 0.1-0.3 part of methionine, 0.1-0.5 part of lysine and 0.012-0.02 part of coenzyme Q.
2. The compound feed according to claim 1, wherein the compound feed is prepared from the following materials in parts by weight: 5 parts of fish meal, 8 parts of chicken powder, 28 parts of bean pulp, 11 parts of rapeseed meal, 8 parts of cottonseed meal, 8 parts of flour, 9 parts of wheat middling, 9 parts of bran, 3 parts of soybean oil, 1 part of multi-vitamin multi-mineral premix, 0.2 part of choline, 1 part of sodium carboxymethylcellulose, 5.338 parts of bentonite, 2 parts of monocalcium phosphate, 0.15 part of methionine, 0.25 part of lysine and 10.012 parts of coenzyme Q.
3. The compound feed according to claim 1 or 2, characterized in that fish meal, chicken meal, soybean meal, rapeseed meal, cottonseed meal, flour, wheat middling, bran, multi-vitamin multi-mineral premix, choline, sodium carboxymethylcellulose, bentonite, monocalcium phosphate, methionine, lysine and coenzyme Q10 are respectively crushed, sieved by a 40-mesh sieve, weighed according to a proportion, fully stirred and uniformly mixed with soybean oil by adopting a step-by-step amplification method, extruded into long strips with the thickness of 1.5 mm-5 mm by a pellet feed granulator, cut into pellets with the length of 3 mm-10 mm, dried at 50 ℃ for 18 hours and then refrigerated.
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