CN115615956A - Super-resolution imaging system and method based on all-fiber lattice interference - Google Patents
Super-resolution imaging system and method based on all-fiber lattice interference Download PDFInfo
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- CN115615956A CN115615956A CN202211027120.1A CN202211027120A CN115615956A CN 115615956 A CN115615956 A CN 115615956A CN 202211027120 A CN202211027120 A CN 202211027120A CN 115615956 A CN115615956 A CN 115615956A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
Abstract
The invention discloses a super-resolution imaging system and a super-resolution imaging method based on all-fiber lattice interference, wherein the imaging system comprises: the light source module is used for emitting linear polarization laser beams with different wavelengths; a beam splitting and phase shifting module including a beam splitting unit for receiving the linearly polarized laser beam and splitting it into a first beam, a second beam and a third beam, and a phase shifting unit for changing phases of the first beam and the second beam; the microscope module is used for receiving the first light beam, the second light beam and the third light beam, irradiating the first light beam, the second light beam and the third light beam on the sample to form an interference pattern, and collecting a fluorescence signal excited by the sample; the imaging module is used for receiving the fluorescence signals collected by the microscope module and imaging; the phase shifting unit comprises the polarization maintaining optical fiber and piezoelectric ceramics, the middle part of the polarization maintaining optical fiber is wound on the piezoelectric ceramics, the phase of the first light beam and the phase of the second light beam in the polarization maintaining optical fiber can be changed by applying voltage to the piezoelectric ceramics, the phase shifting speed is high, and the imaging speed can be improved.
Description
Technical Field
The invention relates to the technical field of imaging systems, in particular to a super-resolution imaging system and method based on all-fiber lattice interference.
Background
The structured light microscope (SIM) can extract high spatial frequency information from a plurality of original images to improve the resolution, so that the optical diffraction limit is broken through, and the SIM is a key technology for realizing high resolution and super-resolution imaging of biological cells and even molecules. Super-resolution SIM systems can typically double the spatial resolution of optical microscopy to show organelles and substructures below 200 nm. Super-resolution SIM systems have been able to simultaneously observe cells in polychromatic optical channels over a longer period of time, minimizing the photobleaching effects that are detrimental to the sample.
Currently, most commercial SIM microscope systems generate ± 1 st order diffracted beams through a diffraction grating, and project them onto the focal plane of a fluorescence microscope to generate interference patterns. For example, in the N-SIM series of the Nikon super-resolution microscope, a mechanical platform is used to control a grating to generate stripes with different directions and phases, but the method is limited by the problems of the operation speed of a mechanical structure, mechanical vibration and the like, and the imaging speed is slow and the stability is poor in the method, whereas in the Elyra 7 super-resolution microscope of Zeiss, a galvanometer structure is used to perform phase shifting, and the galvanometer speed is higher, but the optical path structure is complex and the device cost is too high. In addition, a Spatial Light Modulator (SLM) can be used to change the interference pattern instead of the diffraction grating, but the SLM has a low utilization rate of laser light, usually less than 10%, which puts high demands on the power of the laser.
Disclosure of Invention
The invention has been made in view of the above problems, and an object of the invention is to provide a super-resolution imaging system and method based on all-fiber lattice interference, which can shift the phase of two light beams by changing the voltage of piezoelectric ceramics, and has the advantages of compact structure, simple optical path, fast imaging speed, and good stability.
In order to achieve the above object, the present invention provides a super-resolution imaging system based on all-fiber lattice interference, comprising:
the light source module is used for emitting linear polarized laser beams with different wavelengths;
a beam splitting and phase shifting module including a beam splitting unit for receiving the linearly polarized laser beam and splitting it into a first beam, a second beam and a third beam, and a phase shifting unit for changing phases of the first beam and the second beam;
the collimation and polarization adjustment module is used for collimating the first light beam, the second light beam and the third light beam which pass through the phase shifting unit and changing the polarization direction;
the microscope module is used for receiving the first light beam, the second light beam and the third light beam which pass through the collimation and polarization adjustment module, irradiating the first light beam, the second light beam and the third light beam on a sample to form an interference pattern, and collecting a fluorescence signal excited by the sample;
and the imaging module is used for receiving the fluorescence signals collected by the microscope module and imaging.
According to the super-resolution imaging system based on all-fiber lattice interference, the light source module comprises a multi-color laser, and the multi-color laser is used for emitting linear polarized laser beams with different wavelengths.
According to the super-resolution imaging system based on all-fiber lattice interference, the beam splitting unit comprises a beam splitter for receiving the linearly polarized laser beam emitted by the multicolor laser; the phase shift unit comprises three piezoelectric ceramics and three polarization maintaining optical fibers;
the beam splitter is further configured to split the linearly polarized laser beam into a first beam, a second beam, and a third beam having equal intensities, and transmit the first beam, the second beam, and the third beam to the collimation and polarization adjustment module through the three polarization maintaining fibers, respectively;
the three piezoelectric ceramics are all positioned between the beam splitter and the collimation and polarization adjusting module, and the middle parts of the three polarization maintaining optical fibers are respectively wound on the outer sides of the three piezoelectric ceramics.
According to the full-fiber lattice interference-based super-resolution imaging system, the three polarization maintaining optical fibers are wound on the three piezoelectric ceramics in the same number of turns.
According to the all-fiber lattice interference-based super-resolution imaging system, the beam splitting and phase shifting module further comprises a support, the support is located between the three piezoelectric ceramics and the collimation and polarization adjustment module, a lantern ring for the three polarization-maintaining optical fibers to pass through is arranged on the support, and the diameter of the lantern ring is the same as that of the polarization-maintaining optical fibers;
the three lantern rings are arranged on the bracket in a regular triangle shape.
According to the above full-fiber lattice interference-based super-resolution imaging system, the collimation and polarization adjustment module includes a collimation beam expander and a polarizer, the collimation beam expander is configured to receive a first light beam, a second light beam, and a third light beam of the three polarization-maintaining fibers, and can be configured to expand and collimate the first light beam, the second light beam, and the third light beam, and the polarizer is configured to change polarization directions of the first light beam, the second light beam, and the third light beam passing through the collimation beam expander.
According to the full-fiber lattice interference-based super-resolution imaging system, the microscope module comprises a lens, a reflector, a first tube mirror, a dichroic mirror and an objective lens, and the first light beam, the second light beam and the third light beam sequentially pass through the lens, the reflector, the first tube mirror, the dichroic mirror and the objective lens to irradiate a sample and form an interference pattern.
According to the full-fiber lattice interference-based super-resolution imaging system, the imaging module comprises a filter, a second tube mirror, a camera, an acquisition card and a computer which are sequentially far away from the dichroic mirror, a fluorescence signal generated on the surface of a sample sequentially enters the camera through the objective lens, the dichroic mirror, the optical filter and the second tube mirror, and the camera is used for imaging the fluorescence signal;
and the computer outputs signals through a data acquisition card, is used for controlling the multicolor laser, the piezoelectric ceramics and the camera to work synchronously, and is used for calculating to obtain a super-resolution image.
A super-resolution imaging method based on all-fiber lattice interference comprises the following steps:
s1: dividing light beams emitted by a multicolor laser into a first light beam, a second light beam and a third light beam, arranging the divided first light beam, second light beam and third light beam in a regular triangle, creating images of three point light sources on the back pupil surface of an objective lens, and generating a hexagonal lattice-shaped interference pattern on the surface of a sample through the objective lens;
s2: the first light beam and the second light beam are subjected to phase shifting treatment through piezoelectric ceramics, so that the interference pattern moves transversely;
s3: the camera collects fluorescence signals, and the computer processes the signals through a super-resolution reconstruction algorithm to obtain a super-resolution image.
According to the above-mentioned super-resolution imaging method based on all-fiber lattice interference, in S2, the phase shift step length of the phase difference between the first beam and the second beam is
The phase shift period of the phase difference between the first beam and the second beam is 2 pi, and the phase shift step of the phase difference between the second beam and the third beam is
The second beam is out of phase with the third beam for a phase shift period of 4 pi.
The invention has the following beneficial effects:
1. the current with different voltages is provided for the piezoelectric ceramics, so that two light beams can be subjected to phase shifting, the phase shifting speed is high, the direction of interference fringes does not need to be changed, and the imaging speed can be improved;
2. only the piezoelectric ceramics are added, other phase-shifting mechanisms can be omitted, the overall occupied space of the system can be reduced, and meanwhile, the cost can be reduced;
3. the light beams are transmitted in the optical fiber, so that the interference of external stray light can be avoided as much as possible, the utilization rate of light energy is high, and the requirement on the power of a laser is reduced;
4. the three beams form regular triangle optical axis arrangement through the bracket, and finally the three polarized beams generate a hexagonal lattice-shaped illumination pattern with high contrast on the surface of the sample, so that the sample is clearer.
Drawings
FIG. 1 is an overall optical path system diagram of the present invention;
FIG. 2 is a schematic view of the assembly of the polarizing fiber of the present invention with a piezoelectric ceramic;
FIG. 3 is a schematic view of the stent structure of the present invention;
FIG. 4 is a schematic view of the polarizer construction of the present invention;
fig. 5 is a flow chart of an imaging method of the present invention.
In the figure: 1. a multi-color laser; 2. a beam splitter; 3. a polarization maintaining fiber; 4. piezoelectric ceramics; 5. a support; 51. a collar; 6. a collimated beam expander; 7. a polarizing plate; 8. a lens; 9. a mirror; 10. a first tube lens; 11. a dichroic mirror; 12. an objective lens; 13. a filter plate; 14. a second tube lens; 15. a camera; 16. collecting a card; 17. and (4) a computer.
Detailed Description
The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.
As shown in fig. 1 to 5, a super-resolution imaging system based on all-fiber lattice interference includes:
the light source module is configured to emit linearly polarized laser beams with different wavelengths, and in this embodiment, the wavelengths of the linearly polarized laser beams emitted by the light source module are 405nm, 488nm, 561nm, and 640nm.
The beam splitting and phase shifting module comprises a beam splitting unit and a phase shifting unit, wherein the beam splitting unit is used for receiving the linear polarization laser beam and splitting the linear polarization laser beam into a first light beam, a second light beam and a third light beam, the phase shifting unit is used for changing the phase of the first light beam and the second light beam, the beam splitting unit divides the linear polarization laser beam emitted by the light source module into three light beams, the phase of two light beams can be changed through the phase shifting unit, the phase of the third light beam is not changed, and then an image irradiated on a sample is transversely moved.
And the collimation and polarization adjustment module is used for collimating the first light beam, the second light beam and the third light beam which pass through the phase shift unit and changing the polarization direction, and processing the three light beams by changing the polarization directions after expanding and collimating the first light beam, the second light beam and the third light beam, so that the polarization states of the three light beams are the same, preferably tangential polarization is realized, and the contrast ratio of interference fringes can be improved.
And the microscope module is used for receiving the first light beam, the second light beam and the third light beam which pass through the collimation and polarization adjustment module, irradiating the first light beam, the second light beam and the third light beam on a sample to form an interference pattern and collecting a fluorescence signal excited by the sample, wherein the three light beams can be irradiated on the surface of the sample after being amplified by the microscope module to form an interference intensity pattern, and the fluorescence signal excited by the surface of the sample can be transmitted out again through the microscope module.
And the imaging module is used for receiving the fluorescence signals collected by the microscope module, imaging and then obtaining a super-resolution image according to calculation.
Preferably, the light source module comprises a multi-color laser 1, said multi-color laser 1 being adapted to emit linearly polarized laser beams of different wavelengths, the laser beams of different wavelengths being emitted by the multi-color laser 1.
Further preferably, the beam splitting unit comprises a beam splitter 2 for receiving the linearly polarized laser beam emitted by said polychromatic laser 1; the beam splitter 2 is further configured to split the linearly polarized laser beam into a first beam, a second beam, and a third beam having equal intensities, and transmit the first beam, the second beam, and the third beam to the collimation and polarization adjustment module through the three polarization-maintaining fibers 3, respectively, and the beams are transmitted through the fibers, so that interference of external stray light can be avoided, the utilization rate of light energy is high, and the requirement on the power of the multi-color laser 1 is reduced, wherein the intensities of the first beam, the second beam, and the third beam are equal, and it is ensured that the intensities of the interference patterns are uniform.
The phase shifting unit comprises three piezoelectric ceramics 4 and three polarization maintaining optical fibers 3, the three piezoelectric ceramics 4 are located between the beam splitter 2 and the collimation and polarization adjusting module, the middle parts of the three polarization maintaining optical fibers 3 are wound on the outer sides of the three piezoelectric ceramics 4 respectively, the first light beam, the second light beam and the third light beam correspond to the three polarization maintaining optical fibers 3 one by one, the types of the three piezoelectric ceramics 4 are consistent, voltage is introduced into the piezoelectric ceramics 4 corresponding to the first light beam and the second light beam, and the piezoelectric ceramics 4 generate radial expansion in direct proportion to the voltage, so that space phase change is generated, and phase adjustment of the light beams is realized.
Further preferably, the three polarization maintaining optical fibers 3 are wound on the three piezoelectric ceramics 4 in the same number of turns, and the three polarization maintaining optical fibers 3 are wound in the same manner for the same number of turns, so as to ensure that the initial phase is the same when the zero voltage is applied and the ratio of the phase variation to the loading voltage is the same when the phase is shifted.
Preferably, the beam splitting and phase shifting module further includes a support 5, the support 5 is located between the three piezoelectric ceramics 4 and the collimation and polarization adjustment module, a collar 51 for the three polarization maintaining fibers 3 to pass through is arranged on the support 5, the diameter of the collar 51 is the same as that of the polarization maintaining fibers 3, the three collars 51 are arranged on the support 5 in a regular triangle shape, the support 5 is used for fixing the three polarization maintaining fibers 3 and providing support for the three polarization maintaining fibers 3, and the three polarization maintaining fibers 3 are arranged in a regular triangle shape, so that the three light beams are also arranged in a regular triangle shape, and an image of three point light sources is created on the back pupil plane of the microscope objective 12, thereby generating a hexagonal lattice-shaped interference pattern on the surface of the sample, and facilitating observation.
Preferably, the collimation and polarization adjustment module includes a collimation beam expander 6 and a polarizer 7, the collimation beam expander 6 is configured to receive the first light beam, the second light beam, and the third light beam in the three polarization-maintaining fibers 3, and may be configured to perform beam expansion and collimation on the first light beam, the second light beam, and the third light beam, and the polarizer 7 is configured to change the polarization direction of the first light beam, the second light beam, and the third light beam passing through the collimation beam expander 6.
Further preferably, the microscope module comprises a lens 8, a reflecting mirror 9, a first tube mirror 10, a dichroic mirror 11 and an objective lens 12, the first light beam, the second light beam and the third light beam are irradiated onto the sample through the lens 8, the reflecting mirror 9, the first tube mirror 10, the dichroic mirror 11 and the objective lens 12 in sequence and form an interference pattern, the reflecting mirror 9 reflects the three tangentially polarized light beams into the first tube mirror 10, the first tube mirror 10 images the three tangentially polarized light beams onto a back pupil surface of the objective lens 12, the dichroic mirror 11 reflects the light beams into the objective lens 12 and converges the light beams on the surface of the sample, and the objective lens 12 adopts the objective lens 12 with the magnification of one hundred times and the vertical aperture of 1.49 to collect the three light beams and irradiate on the surface of the sample to form an interference intensity pattern.
Further preferably, the imaging module includes a filter 13, a second tube mirror 14, a camera 15, an acquisition card 16 and a computer 17 which are sequentially away from the dichroic mirror 11, wherein a fluorescent signal generated on the surface of the sample sequentially enters the camera 15 through the objective lens 12, the dichroic mirror 11, the filter and the second tube mirror 14, the camera 15 is used for imaging the fluorescent signal, the dichroic mirror 11 transmits the fluorescent signal to the filter after the objective lens 12 collects the fluorescent signal generated by the sample, the filter is used for emitting stray light and part of projected laser in the fluorescent signal and corresponds to laser with four wavelengths in the multicolor laser 1, so that the filter 13 with four different wavelength ranges is needed, and a high-speed filter wheel can be used for switching the filter, and the computer 17 is used for controlling the wheel, and the second tube mirror 14 is used for imaging the intensity information of the fluorescent signal into a connection; the computer 17 outputs signals through the data acquisition card 16, and is used for controlling the multicolor laser 1, the piezoelectric ceramic 4 and the camera 15 to work synchronously and calculating to obtain a super-resolution image, and the calculation adopts a structured light reconstruction algorithm.
A super-resolution imaging method based on all-fiber lattice interference comprises the following steps:
s1: the light beam emitted by the laser emitter is divided into a first light beam, a second light beam and a third light beam, the divided first light beam, second light beam and third light beam are arranged in a regular triangle, images of three point light sources are created on the back pupil surface of the objective lens 12, and a hexagonal lattice-shaped interference pattern is generated on the surface of a sample through the objective lens 12.
S2: the first and second beams are phase-shifted by the piezoelectric ceramics 4 to shift the interference pattern laterally.
S3: the camera 15 collects the fluorescence signal, and the computer 17 processes the signal through a super-resolution reconstruction algorithm to obtain a super-resolution image.
Preferably, in S2, the phase shift step of the phase difference between the first beam and the second beam is
The phase shift period of the phase difference between the first and second beams is 2 pi, and the phase shift step of the phase difference between the second and third beams is
The second beam is out of phase with the third beam for a phase shift period of 4 pi, wherein the third beam is not phase shifted; the relative phase at each step in the phase shifting process is shown in the following table:
TABLE 1
Table 1 shows the relative phases of the first, second and third beams during phase shifting; where k denotes the number of phase shifting steps,
indicating the phase difference of the ith beam relative to the jth beam.
The nth phase shifted image of the hexagonal lattice-like interference pattern can be represented by the following equation:
k m is a frequency vector, tableThe form is as follows:
wherein a is the distance between rows of adjacent bright spots, and the bright spots can be deconstructed from an image acquired by a camera; the above-mentioned n phase shift image equation contains 7 unknowns
Where O is the sample function, h is the optical transfer function of the imaging system, α m In order to be the amplitude of the vibration,
the image is an initial absolute phase, so that 7 original images are needed to calculate a super-resolution image, and the image is different from 9 original images in the prior art, and the imaging speed is higher; further, κ is a constant for determining the shift direction, and taking κ =2 can minimize the error propagation of the direction angle, in this case, the intensity formula of the phase-shifted image can be written as:
solving the above equation can yield:
from the light represented by the four sets of solutions described above, which can be applied to hexagonal structured light illumination, we determine the minimum step size required for phase shifting as
The minimum period is 2 pi.
The technical solutions of the present invention are explained in detail above with reference to the accompanying drawings, and the described embodiments are used to help understanding the idea of the present invention. The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.
It should be noted that all directional indicators (such as up, down, left, right, front, back \8230;) in the embodiments of the present invention are only used to explain the relative positional relationship between the components, the motion situation, etc. in a specific posture (as shown in the attached drawings), and if the specific posture is changed, the directional indicator is changed accordingly.
Moreover, descriptions of the present invention as relating to "first," "second," "a," etc. are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit ly indicating a number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "connected," "secured," and the like are to be construed broadly, and for example, "secured" may be a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.
In addition, the technical solutions in the embodiments of the present invention may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination of technical solutions should not be considered to exist, and is not within the protection scope of the present invention.
Claims (10)
1. A super-resolution imaging system based on full-fiber lattice interference is characterized by comprising:
the light source module is used for emitting linear polarized laser beams with different wavelengths;
a beam splitting and phase shifting module including a beam splitting unit for receiving the linearly polarized laser beam and splitting it into a first beam, a second beam, and a third beam, and a phase shifting unit for changing phases of the first beam and the second beam;
the collimation and polarization adjustment module is used for collimating the first light beam, the second light beam and the third light beam which pass through the phase shifting unit and changing the polarization direction;
the microscope module is used for receiving the first light beam, the second light beam and the third light beam which pass through the collimation and polarization adjustment module, irradiating the first light beam, the second light beam and the third light beam on a sample to form an interference pattern, and collecting a fluorescence signal excited by the sample;
and the imaging module is used for receiving the fluorescence signals collected by the microscope module and imaging.
2. The system of claim 1, wherein the light source module comprises a multi-color laser for emitting linearly polarized laser beams with different wavelengths.
3. The system of claim 2, wherein the beam splitting unit comprises a beam splitter for receiving the linearly polarized laser beam from the multi-color laser; the phase shift unit comprises three piezoelectric ceramics and three polarization maintaining optical fibers;
the beam splitter is further configured to split the linearly polarized laser beam into a first beam, a second beam, and a third beam having equal intensities, and transmit the first beam, the second beam, and the third beam to the collimation and polarization adjustment module through the three polarization maintaining fibers, respectively;
the three piezoelectric ceramics are all positioned between the beam splitter and the collimation and polarization adjusting module, and the middle parts of the three polarization maintaining optical fibers are respectively wound on the outer sides of the three piezoelectric ceramics.
4. The system of claim 3, wherein three polarization maintaining fibers are wound on three piezoelectric ceramics in the same number of turns.
5. The system according to claim 3, wherein the beam splitting and phase shifting module further comprises a support, the support is located between the three piezoelectric ceramics and the collimating and polarization adjusting module, the support is provided with a collar for passing three polarization-maintaining fibers, and the diameter of the collar is the same as that of the polarization-maintaining fibers;
the three lantern rings are arranged on the bracket in a regular triangle shape.
6. The system of claim 3, wherein the collimation and polarization adjustment module comprises a collimation beam expander and a polarizer, the collimation beam expander is configured to receive the first beam, the second beam, and the third beam from the three polarization-maintaining fibers, and is configured to expand and collimate the first beam, the second beam, and the third beam, and the polarizer is configured to change the polarization direction of the first beam, the second beam, and the third beam passing through the collimation beam expander.
7. The system according to claim 6, wherein the microscope module comprises a lens, a reflector, a first tube mirror, a dichroic mirror, and an objective lens, and the first, second, and third light beams sequentially pass through the lens, the reflector, the first tube mirror, the dichroic mirror, and the objective lens to irradiate onto the sample and form the interference pattern.
8. The system of claim 7, wherein the imaging module comprises a filter, a second tube lens, a camera, an acquisition card and a computer, the filter, the second tube lens, the camera and the computer are sequentially away from the dichroic mirror, a fluorescence signal generated on the surface of the sample sequentially enters the camera through the objective lens, the dichroic mirror, the filter and the second tube lens, and the camera is used for imaging the fluorescence signal;
and the computer outputs signals through a data acquisition card, is used for controlling the multicolor laser, the piezoelectric ceramics and the camera to work synchronously, and is used for calculating to obtain a super-resolution image.
9. A super-resolution imaging method based on all-fiber lattice interference is characterized by comprising the following steps:
s1: dividing light beams emitted by a multicolor laser into a first light beam, a second light beam and a third light beam, arranging the divided first light beam, second light beam and third light beam in a regular triangle, creating images of three point light sources on the back pupil surface of an objective lens, and generating a hexagonal lattice-shaped interference pattern on the surface of a sample through the objective lens;
s2: the first light beam and the second light beam are subjected to phase shifting treatment through piezoelectric ceramics, so that the interference pattern moves transversely;
s3: the camera collects fluorescence signals, and the computer processes the signals through a super-resolution reconstruction algorithm to obtain a super-resolution image.
10. The method of claim 9, wherein in S2, the phase shift step of the phase difference between the first beam and the second beam is as follows
The phase shift period of the phase difference between the first beam and the second beam is 2 pi, and the phase shift step of the phase difference between the second beam and the third beam is
The second beam is out of phase with the third beam for a phase shift period of 4 pi.
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