CN115613542A - Method for promoting calcium bloom restoration by submerged plants - Google Patents
Method for promoting calcium bloom restoration by submerged plants Download PDFInfo
- Publication number
- CN115613542A CN115613542A CN202211397109.4A CN202211397109A CN115613542A CN 115613542 A CN115613542 A CN 115613542A CN 202211397109 A CN202211397109 A CN 202211397109A CN 115613542 A CN115613542 A CN 115613542A
- Authority
- CN
- China
- Prior art keywords
- bloom
- calcium
- calcium bloom
- deposition
- restoration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011575 calcium Substances 0.000 title claims abstract description 108
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 title claims abstract description 75
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000001737 promoting effect Effects 0.000 title claims abstract description 11
- 239000013078 crystal Substances 0.000 claims abstract description 62
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 49
- 241000196324 Embryophyta Species 0.000 claims abstract description 41
- 241000543445 Vallisneria spiralis Species 0.000 claims abstract description 22
- 241001123263 Zostera Species 0.000 claims abstract description 15
- 230000008569 process Effects 0.000 claims abstract description 3
- 241000195940 Bryophyta Species 0.000 claims description 7
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 7
- 229910001424 calcium ion Inorganic materials 0.000 claims description 7
- 238000004062 sedimentation Methods 0.000 claims description 4
- 230000008439 repair process Effects 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 claims description 2
- 102000007590 Calpain Human genes 0.000 claims 1
- 108010032088 Calpain Proteins 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 230000008021 deposition Effects 0.000 abstract description 37
- 241000195493 Cryptophyta Species 0.000 abstract description 20
- 238000002441 X-ray diffraction Methods 0.000 abstract description 16
- 241000252229 Carassius auratus Species 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 9
- MUKYLHIZBOASDM-UHFFFAOYSA-N 2-[carbamimidoyl(methyl)amino]acetic acid 2,3,4,5,6-pentahydroxyhexanoic acid Chemical compound NC(=N)N(C)CC(O)=O.OCC(O)C(O)C(O)C(O)C(O)=O MUKYLHIZBOASDM-UHFFFAOYSA-N 0.000 abstract description 7
- 241000159213 Zygophyllaceae Species 0.000 abstract description 7
- 235000009165 saligot Nutrition 0.000 abstract description 7
- 230000008859 change Effects 0.000 abstract description 6
- 241000251468 Actinopterygii Species 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 4
- 239000012620 biological material Substances 0.000 abstract description 3
- 241000209443 Ceratophyllum demersum Species 0.000 abstract description 2
- 238000001228 spectrum Methods 0.000 abstract description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 19
- 239000002131 composite material Substances 0.000 description 18
- 239000002244 precipitate Substances 0.000 description 18
- 239000002245 particle Substances 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 9
- 235000002639 sodium chloride Nutrition 0.000 description 7
- 229910021532 Calcite Inorganic materials 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 230000029553 photosynthesis Effects 0.000 description 4
- 238000010672 photosynthesis Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000002308 calcification Effects 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000005137 deposition process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 241000195967 Anthoceros Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000252233 Cyprinus carpio Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000001878 scanning electron micrograph Methods 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000206751 Chrysophyceae Species 0.000 description 1
- 235000002722 Dioscorea batatas Nutrition 0.000 description 1
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 1
- 240000001811 Dioscorea oppositifolia Species 0.000 description 1
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 1
- 241001523348 Picrasma Species 0.000 description 1
- 240000004659 Picrasma quassioides Species 0.000 description 1
- 235000010913 Picrasma quassioides Nutrition 0.000 description 1
- 241000877996 Vallisneria natans Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 101150026370 calua gene Proteins 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000009372 pisciculture Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Images
Classifications
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Structural Engineering (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Agronomy & Crop Science (AREA)
- Mining & Mineral Resources (AREA)
- Paleontology (AREA)
- Civil Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses a method for promoting the restoration of caltrop by submerged plants, belonging to the technical field of geological restoration. The invention takes eel grass (Vallisianatana Hara) and golden fish algae (Ceratophyllum demersum L.) as the repairing biological material to promote the deposition of the calcium bloom, analyze the planting density of different plants and Ca in different water bodies 2+ The effect of submerged plants on the deposition of caltrop bloom. From the measured Ca 2+ The concentration adopts an attenuation index equation to fit the calcium bloom deposition kinetic process, and the appearance and the structure of the calcium bloom crystal are characterized and analyzed by combining an SEM (scanning electron microscope) and an XRD (X-ray diffraction) spectrum, the submerged plant restoration technology can accelerate the deposition rate of the calcium bloom and change the crystal appearance, and the calcium bloom crystal appearance of the tape grass group is better and more regular in development compared with the goldfish algae group.
Description
Technical Field
The invention relates to the technical field of geological restoration, in particular to a method for promoting restoration of calvities by submerged plants.
Background
The landform is a natural peculiar landscape and is an important constituent part of karst landform in the world. The Chinese yam Huanglong and Yunnan Baishui platform are famous for the rich calcium bloom deposition landforms in China, and attract vast tourists and scholars to watch and study. However, in recent years, due to factors such as natural environment changes and artificial damages, some of the calcium bloom landforms have been changed or even degraded to different degrees, and have attracted extensive attention.
The influence factors of the deposition of calcium bloom are various, and the influence of aquatic plants is very important. In the research progress on the deposition process of calcium bloom, the influence of biological causes on the formation of calcium bloom has been of great concern. The physical and chemical characteristics and biological actions of submerged plants, which are aquatic plants growing in water all the time, influence the deposition process of the calsium. However, there is no detailed research on how the submerged plant affects the micro-morphology and crystal structure of the calcium bloom and the growth change rule of the submerged plant in the calcium bloom water pool.
At present, the influence of submerged plants on the calcium bloom is researched and lacked, and the calcium bloom conservation work is extremely important in recent years due to the degeneration condition of the calcium bloom. In order to protect and prevent the calcium bloom from degeneration, the method for promoting the calcium bloom repair by using submerged plants is significant.
Disclosure of Invention
The invention aims to provide a method for promoting the restoration of calcium bloom by using submerged plants, so as to solve the defects in the prior art. The invention takes eel grass (Vallisneria natans Hara) and golden carp algae (Ceratophyllum demersum L.) as repairing biological materials to promote the deposition of calcium bloom, analyze the planting density of different plants and Ca in different water bodies 2+ The effect of concentration-submerged plants on the deposition of calcium bloom.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a method for promoting calcium bloom restoration by using submerged plants, which is characterized in that the submerged plants are added into a water body containing calcium ions for culture to accelerate the sedimentation of calcium bloom crystals in the water body.
Preferably, the concentration of the calcium ions is 100 to 500mg/L.
Preferably, the other ion concentrations of the solution are: mg (Mg) 2+ ＝20mg/L、HCO 3 － ＝720mg/L、 SO 4 2- ＝30mg/L。
Preferably, the culture conditions are: the pH was set at 7.0. The culture temperature is indoor natural temperature, and the light-dark ratio is 12h: and (4) 12h.
Preferably, the submerged plants include tape grass and hornworts.
Preferably, the adding amount of the tape grass is 5 to 30 strains/3.5L.
Preferably, the addition amount of the hornworts is 5-30 strains/3.5L.
The invention also provides a product prepared by the method.
The invention also provides application of the product in promoting the restoration of the calcium bloom.
The method for promoting the restoration of the calvital by using the submerged plants has the advantages and positive effects that:
the invention takes submerged plants of tape grass and goldfish algae as biological materials to promote the deposition of the calcium bloom and repair the landscape of the calcium bloom. Screening the planting density of different plants and the initial Ca of different water bodies 2+ Comparing the concentrations, performing dynamic fitting on the calcium bloom deposition, characterizing the morphology and structure of the calcium bloom crystal by a scanning electron microscope and XRD (X-ray diffractometer technology), and measuring Ca content 2+ The concentration adopts an attenuation index equation to fit the calcium bloom deposition kinetic process, and the appearance and the structure of the calcium bloom crystal are characterized and analyzed by combining an SEM (scanning electron microscope) and an XRD (X-ray diffraction) spectrum, the submerged plant restoration technology can accelerate the deposition rate of the calcium bloom and change the appearance of the crystal, and the appearance of the calcium bloom crystal of the tape grass group is better and more regular in development compared with the goldfish algae group.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is SEM image of the deposition of calcium bloom in tape grass, wherein A is 5 strains; 10 strains of B; c, 15 strains; d, 30 strains;
FIG. 2 is an SEM image of algal tufa calcium bloom deposition, wherein A is 5 strains; b, 10 strains; 15 strains; and D, 30 strains.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein methods, unless otherwise specified, are generally performed using conventional methods, and reagents, unless otherwise specified, are generally commercially available or configured using conventional methods. This detailed description is not to be taken in a limiting sense, but is to be understood as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in the present disclosure, it is understood that each intervening value, to the upper and lower limit of that range, is also specifically disclosed. Every intervening value, to the extent any stated value or intervening value in a stated range, and any other stated or intervening value in a stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only the preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
Photosynthesis and respiration of submerged plants affect CO in water 2 Changes occur in the day and night. Because the leaves of the submerged plant have more chloroplasts, the illumination in water can be fully utilized in the daytime to carry out photosynthesis. During photosynthesis, the submerged plants are treated with HCO in water 3 － And CO 2 As the main carbon source, CO is added 2 Separated from the solution to reduce inorganic carbon and CO 2 The content in water is converted into biological organic substances, and CO is reduced 2 Partial pressure in water and increasing the pH of the solution, promotes deposition of calcium bloom. When submerged plants perform respiration at night, CO is released 2 The pH of the water body is reduced, so that CO is generated 2 The partial pressure in the water increases, thereby slowing down the deposition rate of the calsium.
In addition, the branches and leaves of the submerged plant and the root system thereof can absorb pollutants in water, and microorganisms can effectively degrade and remove pollutants through the root system. Meanwhile, the submerged plant has strong capability of absorbing and storing water body nutrient substances, so that the eutrophication phenomenon of the water body can be prevented and treated, and the calcium bloom is prevented from being degraded. Therefore, the submerged plant can achieve the good effects of improving the water body environment and improving the self-purification capacity of the water body, and has good promotion effect on the calcium bloom deposition.
Ultrapure water and CaCl used in laboratory for water body in experiment 2 、MgCl 2 、NaHCO 3 、K 2 SO 4 Is configured according to the set concentration.
Water body Ca 2+ And (3) concentration determination: 12mL of experiment group solution is sequentially sucked by a liquid transfer gun and stored in a centrifugal tube, every 2d is sequentially taken, a label is pasted and placed in a refrigerator for cold storage and storage, and the sample is sent to a Chengdu all Hui Biotech limited company for determination by using an atomic absorption spectrometer. The culture temperature is indoor natural temperature, and the light-dark ratio is 12h:12h, setting the total precipitation period of the calsium to be 15d, setting the measurement time of each index of the water body to be 12d, and measuring each index of the water body and Ca every 2d 2+ And (4) concentration.
Characterization and analysis of a scanning electron microscope and XRD: after the caltrop precipitation period, all plants containing 30 plants and all Ca were collected 2+ Putting all the glass slides in a group with the concentration of 300mg/L into an oven to be dried for two hours, selecting one glass slide from each dried sample to carry out scanning electron microscope observation on the morphology of the calcium bloom crystal, scraping the precipitate by using a knife on the rest glass slides, collecting the precipitate into a 5mL centrifugal tube, and sending the centrifugal tube to Brookfield science and technology Limited company for XRD calcium bloom crystal structureAnd (6) analyzing.
Example 1
(1) Separately prepare Ca 2+ The concentration of the composite water body is 100mg/L, 300mg/L and 500 mg/L;
(2) Preparation of 4 groups of prepared Ca 2+ Adding the tape grass into the composite water body with the concentration of 100mg/L, wherein the volume of each group of composite water body is 3.5L, and the adding amount of the tape grass in 4 groups of composite water bodies is respectively 5, 10, 20 and 30 strains;
(3) Sequentially adding Ca according to the method in the step (2) 2+ Adding tape grass into the composite water body with the concentration of 300mg/L and 500 mg/L;
(4) Culturing the tape grass-containing composite water body prepared in the steps (2) and (3), wherein the culture conditions are as follows: the pH was set at 7.0. The culture temperature is indoor natural temperature, and the light-dark ratio is 12h: and (4) 12h.
(5) Detection of Ca in composite water body at intervals of 2d 2+ A change in concentration;
(6) Calculating the calcification rate in the composite water body by an exponential decay equation, wherein the calculation formula is as follows:
[Ca 2+ ]＝e kt+b formula (1)
In[Ca 2+ ]= kt + b formula (2)
Wherein [ Ca 2+ ]Represents the calcium ion concentration, t represents the sampling time, and k represents the calsium rate constant.
(7) XRD and SEM detection are respectively carried out on the deposited caltrop crystals.
Example 2
The difference from example 1 is that the eel grass is changed to golden fish algae.
Comparative example 1
(1) Separately prepare Ca 2+ The concentration of the composite water body is 100mg/L, 300mg/L and 500 mg/L;
(2) Culturing the composite water prepared in the step (1) under the culture conditions: the pH value is set to
7.0. The culture temperature is indoor natural temperature, and the light-dark ratio is 12h: and (4) 12h.
(3) Detection of Ca in composite water body at intervals of 2d 2+ A change in concentration;
(4) Calculating the calcification rate in the composite water body by an exponential decay equation, wherein the calculation formula is as follows:
[Ca 2+ ]＝e kt+b formula (1)
In[Ca 2+ ]= kt + b equation (2)
Wherein [ Ca 2+ ]Represents the calcium ion concentration, t represents the sampling time, and k represents the calsium rate constant.
(5) And respectively carrying out XRD (X-ray diffraction) and SEM (scanning Electron microscope) detection on the deposited caltrop crystals.
Comparative example 2
(1) Preparation of Ca 2+ Dividing the composite water body with the concentration of 0 into 4 groups, and respectively adding 5, 10, 20 or 30 tape grass into the 4 groups of composite water bodies;
(2) Culturing the tape grass prepared in the step (1) under the following culture conditions: the pH was set to 7.0.
The culture temperature is indoor natural temperature, and the light-dark ratio is 12h: and (5) 12h.
(3) Detection of Ca in composite water body at intervals of 2d 2+ A change in concentration;
(4) Calculating the calcification rate in the composite water body by an exponential decay equation, wherein the calculation formula is as follows:
[Ca 2+ ]＝e kt+b formula (1)
In[Ca 2+ ]= kt + b equation (2)
Wherein [ Ca ] 2+ ]Represents the calcium ion concentration, t represents the sampling time, and k represents the calsium rate constant.
(5) And respectively carrying out XRD (X-ray diffraction) and SEM (scanning Electron microscope) detection on the deposited caltrop crystals.
Comparative example 3
The difference from comparative example 2 is that the eel grass is changed to hornworts.
The experimental results are as follows:
(1) Kinetic fitting during Calua deposition
The kinetics of deposition of Calori in 12d are fitted to Table 1
TABLE 1
Note: in the table, Y represents: calwaals rate, in units (mg/L. D), x represents: days reacted, units (d).
As can be seen from Table 1, the goodness of fit R 2 The calcium bloom deposition kinetics are all above 0.7, the fitting equation has good linearity, and the deposition kinetics of the calcium bloom follow the exponential decay equation.
Ca 2+ The concentration is 100mg/L, the planting quantity of the tape grass is the maximum calcium bloom deposition rate of 20 strains, and the calcium bloom deposition rate of 30 strains of tape grass is the minimum. The calcium bloom deposition rate of the whole goldfish algae group is high, and the calcium bloom deposition rate of 10 goldfish algae groups reaches the maximum value; ca 2+ When the concentration is 300mg/L, the deposition rate of the calcium bloom in the 30 goldfish algae group is reduced; when Ca is present 2+ At a concentration of 500mg/L, the deposition rate of the calcium bloom is the lowest in the group of 5 tape grass plants, and the deposition rate of the calcium bloom is supposed to be slowed down when the planting density of the submerged plants is smaller or larger.
The deposition kinetics of the calhua of the eel grass group and the goldfish algae group follow the exponential decay equation, and the goodness of fit of the goldfish algae group is integrally higher than that of the eel grass group. When Ca is present 2+ When the concentration is higher, the deposition rate of the calcium bloom in the 30 picrasma and goldfish algae groups is lower. The deposition rate of the whole calhua in the Goldfish algae group is greater than that in the Swingla grass group.
(2) Effect of different planting densities on micro-morphology of calcium bloom
Ca in composite water body 2+ The scanning result of the electron microscope of the eel grass group calcium bloom crystal under the condition of the concentration of 300mg/L is shown in figure 1, and from the scanning electron microscope result, the surface of the eel grass blank group glass sheet is covered with a layer of amorphous calcium carbonate crystal with the grain diameter of 2-4 μm. Wherein a small amount of crystals of the square and petal-shaped calcium carbonate are connected and stacked with each other, and the single crystal has a particle size of about 50 to 70 μm.
The calcium bloom crystal of the 5-plant eel grass group has the best morphology effect, a large amount of amorphous precipitates with the grain diameter less than 1 mu m are arranged on the surface, and the types are not determined. The precipitate contains square calcite crystals with particle size of 10-20 μm, and the crystals are wrapped by amorphous precipitate. There are standard calcite crystals with larger particle sizes, and some crystals with larger particle sizes have obvious step structures, which indicates that when particles are stacked on crystal nuclei, multiple layers are stacked simultaneously.
The calcium bloom surfaces of the 10 bitter herb groups and the 20 bitter herb groups respectively have large-area unshaped precipitates with the grain sizes smaller than 1 mu m, the typical square crystals and long-strip crystals are found in the calcium bloom precipitates of the 10 bitter herb groups, and the calcium bloom precipitates of the 20 bitter herb groups have a small amount of petal-shaped and long-strip crystals.
The 30 picrasma quassioides groups had a thicker packing of precipitates including amorphous precipitates as well as vaterite and square crystals. The vaterite has a particle size of about 2 μm, small square crystals have a particle size of 2-8 μm, and larger square crystals can reach about 40 μm. The square crystals can be obviously observed to have a connected and stepped structure, and the phenomenon that small square crystals develop on the top and around the larger square crystals can be also observed.
Composite water body Ca 2+ As shown in FIG. 2, the development of the calcium bloom crystals of the 30-strain hornwort group was the most excellent in terms of the amount of crystals and the growth of the crystals, as seen in the electron microscope scanning results of the calcium bloom crystals of the hornwort group at a concentration of 300mg/L, and the crystals were mainly in the form of vaterite, droplets and petals, the diameter of the vaterite was about 3 to 8 μm, the crystals were connected to each other to form larger precipitates having a diameter of 400 to 500 μm, and petal-shaped crystals having a diameter of about 10 μm were developed thereon, and they were connected to each other in a scaly structure. Meanwhile, a small amount of square calcite crystals with the grain size of 20-40 mu m exist.
In the calcium bloom precipitates of the 10 and 20 Goldfish algae groups, a large number of amorphous precipitates with a particle size of less than 1 μm and a precipitate formed by the association of vaterite with a diameter of about 1 μm were observed, wherein more petal-shaped crystals were found in the calcium bloom precipitates of the 10 Goldfish algae groups and the lamellar structure was more distinct. The surface of the glass slide of the 5-strain chrysophyceae group was less likely to be covered with amorphous precipitates and had agglomerated crystals. The crystal types comprise vaterite, water drop and petal, the water drop and petal crystal layer structure is obvious, and the grain diameter is 20-30 μm. The diameter of the vaterite is between 2 and 8 μm, and the vaterite is connected into a cluster.
Scanning electron microscope pair Ca 2+ The characterization results of the calcium bloom deposition in the water body with the concentration of 300mg/L show that a small amount of square and petal-shaped calcium carbonate crystals are mutually connected and stacked in the comparison ratio 1. The crystal shape of the comparative example 2 is more regular than that of the comparative example 3, the shape effect of the caltrop crystal of the 5-plant eel grass experimental group is best, and standard calcite crystal with larger particle size exists in the sample precipitate. The samples of the experimental group of the golden pisces have more vaterite, petal-shaped and drop-shaped crystals, and the square calcite crystals and the amorphous crystals are less; and the sedimentation effect of the experimental group of 30 golden pisciculture is the best, and the diameter of the sedimentation particles can reach 400-500 mu m.
(3) Qualitative analysis of XRD calcium bloom crystal structure
XRD characterization of the deposit revealed that samples planted in the 5, 10, 20, 30 eel grass groups of example 1 had calcite as the main component, caCO of the formula 3 The space group is R-3C (167). Samples of the 20 tape grass group were analyzed to crystallize predominantly as salt rock, chemical formula NaCl, space group Fm-3m (225).
The most intense diffraction peaks of the three calcium carbonate crystals of the tape grass groups (5, 10 and 30 plants) appear on the 104 surface, the crystallinity of the 5 tape grass groups is the highest, and the average grain size of the 10 tape grass groups is the largest. Meanwhile, the NaCl content in the sediment of each group of the eel grass is also very high, and compared with the corresponding group of the golden fish algae under the same condition, the crystallinity and the content are both larger. Even 20 tape grass groups were found in which the major component was salt rock. The baselines of the four groups are not flat, the base parts of diffraction peaks are wider, and the four groups of deposits are in an amorphous state, and the interior of a matrix has lattice distortion. The 10 picrass groups had more dominant side deletions and poorer crystal development (see B in fig. 1).
Comparing example 2 with different densities of Goldfish algae (as shown in FIG. 2), the strongest peaks of diffraction all appear on the 104 plane. The crystallinity was highest in the 10 hornworts group, but the average grain size was largest in the 30 hornworts group, while sodium chloride deposition in a semi-crystalline state was observed in each group. The base line of the diffraction peak is less flat, which indicates that the deposit contains more amorphous impurities.
For Ca 2+ The concentration of each group in the water body is 300mg/LPerforming XRD (X-ray diffraction) characteristic analysis on the crystal structures of the calcium bloom precipitates, wherein the crystals of the calcium blooms of the eel grass group and the golden fish algae group are both of a cubic structure with calcium carbonate as a main component; and the crystals of the 20 eel grass groups are found to be parallelepipedal rock salt, the main component is sodium chloride, and the crystallinity and the content of the corresponding groups of the golden carp algae are higher than those of the same case. The crystallinity of 5 picrass groups is the maximum and reaches 92.63 percent; secondly, the crystallinity of 20 goldfish algae groups reaches 88.02 percent; and the greater the density of the two implants, the lower the crystallinity. The baseline in the tape grass group is not flat, and the base of the diffraction peak is wider, which indicates that the four groups of sediments are in an amorphous state, and the crystal inside has lattice distortion.
In summary, the present invention affects CO in water through the main photosynthesis and respiration of submerged plants 2 The partial pressure of the calcium bloom crystal has certain promotion effect on the deposition rate of the calcium bloom and the growth of the calcium bloom crystal. Meanwhile, the planting density of different submerged plants and Ca in the water body are determined by fitting the planting density with the deposition rate of calcium bloom 2+ The concentration of the water-soluble calcium bloom can influence the deposition process of the calcium bloom to different degrees, and meanwhile, the deposition of the calcium bloom can also influence the growth vigor and the life metabolic activities of the submerged plants, so that the deposition of the calcium bloom is further promoted through the submerged plants, and the support is provided for the restoration of the calcium bloom.
Finally, it should be noted that: the above embodiments are only intended to illustrate the technical solution of the present invention and not to limit the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.
Claims (9)
1. A method for promoting the restoration of calcium bloom by using submerged plants is characterized in that the submerged plants are added into a water body containing calcium ions for culture to accelerate the sedimentation of calcium bloom crystals in the water body.
2. The method according to claim 1, wherein the concentration of calcium ion is 100 to 500mg/L.
3. The method of claim 1, wherein the other ion concentrations of the solution are: mg (magnesium) 2+ ＝20mg/L、HCO 3 － ＝720mg/L、SO 4 2- ＝30mg/L。
4. The method of claim 1, wherein the culture conditions are: the pH value is 7.0, the culture temperature is indoor natural temperature, and the light-dark ratio is 12h: and (4) 12h.
5. The method of claim 1, wherein the submerged plants comprise eel grass and hornworts.
6. The method according to claim 4, wherein said tape grass is added in an amount of 5 to 30 strains/3.5L.
7. The method as claimed in claim 4, wherein the amount of the hornworts added is 5 to 30 strains/3.5L.
8. A product obtained by the process of any one of claims 1 to 6.
9. Use of a product according to claim 7 for promoting calpain repair.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211397109.4A CN115613542A (en) | 2022-11-09 | 2022-11-09 | Method for promoting calcium bloom restoration by submerged plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211397109.4A CN115613542A (en) | 2022-11-09 | 2022-11-09 | Method for promoting calcium bloom restoration by submerged plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115613542A true CN115613542A (en) | 2023-01-17 |
Family
ID=84877728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211397109.4A Pending CN115613542A (en) | 2022-11-09 | 2022-11-09 | Method for promoting calcium bloom restoration by submerged plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115613542A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5106504A (en) * | 1991-02-12 | 1992-04-21 | Murray David P | Artificial open water structures |
| US20020023876A1 (en) * | 2000-08-23 | 2002-02-28 | Debusk Thomas A. | Algal and nutrient control system and method for a body of water |
| CN1769198A (en) * | 2005-09-05 | 2006-05-10 | 中国科学院生态环境研究中心 | Method for transferring water bloom to water-bed plant using clay agglomeration |
| US20110086400A1 (en) * | 2009-05-07 | 2011-04-14 | Aquatech Bioenergy LLC | Method and System for Collecting Ethanol from Aquatic Plants |
| CN106430526A (en) * | 2016-10-14 | 2017-02-22 | 台州市绿野环保工程有限公司 | River sewage treatment process |
| CN107169679A (en) * | 2017-06-22 | 2017-09-15 | 北京师范大学 | A kind of method of utilization food web estimation of stability Wetland ecological moisturizing implementation result |
| CN109024461A (en) * | 2018-09-06 | 2018-12-18 | 成都环美园林生态股份有限公司 | A kind of artificial ecology lake construction method for karst landform |
| CN109534765A (en) * | 2018-12-14 | 2019-03-29 | 成都理工大学 | Travertine geology grouting material, preparation method and the technique for repairing underwater travertine geology crack |
| US20200172438A1 (en) * | 2018-02-14 | 2020-06-04 | Dust Biosolutions Gmbh | Preventing or reducing plant growth by biocementation |
| CN112062405A (en) * | 2020-09-08 | 2020-12-11 | 中国科学院南京地理与湖泊研究所 | Landscape lake water replenishing purification process |
| EP3789353A1 (en) * | 2019-09-09 | 2021-03-10 | Nanjing Institute Of Geography & Limnology. Chinese Academy Of Sciences | Ecological remediation method for water bodies rich in high-turbidity colloidal particles |
| CN112607956A (en) * | 2020-11-25 | 2021-04-06 | 中国科学院地球化学研究所 | Ecological restoration and aquatic ecosystem restoration method for polluted water body of plateau lake |
-
2022
- 2022-11-09 CN CN202211397109.4A patent/CN115613542A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5106504A (en) * | 1991-02-12 | 1992-04-21 | Murray David P | Artificial open water structures |
| US20020023876A1 (en) * | 2000-08-23 | 2002-02-28 | Debusk Thomas A. | Algal and nutrient control system and method for a body of water |
| CN1769198A (en) * | 2005-09-05 | 2006-05-10 | 中国科学院生态环境研究中心 | Method for transferring water bloom to water-bed plant using clay agglomeration |
| US20110086400A1 (en) * | 2009-05-07 | 2011-04-14 | Aquatech Bioenergy LLC | Method and System for Collecting Ethanol from Aquatic Plants |
| CN106430526A (en) * | 2016-10-14 | 2017-02-22 | 台州市绿野环保工程有限公司 | River sewage treatment process |
| CN107169679A (en) * | 2017-06-22 | 2017-09-15 | 北京师范大学 | A kind of method of utilization food web estimation of stability Wetland ecological moisturizing implementation result |
| US20200172438A1 (en) * | 2018-02-14 | 2020-06-04 | Dust Biosolutions Gmbh | Preventing or reducing plant growth by biocementation |
| CN109024461A (en) * | 2018-09-06 | 2018-12-18 | 成都环美园林生态股份有限公司 | A kind of artificial ecology lake construction method for karst landform |
| CN109534765A (en) * | 2018-12-14 | 2019-03-29 | 成都理工大学 | Travertine geology grouting material, preparation method and the technique for repairing underwater travertine geology crack |
| EP3789353A1 (en) * | 2019-09-09 | 2021-03-10 | Nanjing Institute Of Geography & Limnology. Chinese Academy Of Sciences | Ecological remediation method for water bodies rich in high-turbidity colloidal particles |
| CN112062405A (en) * | 2020-09-08 | 2020-12-11 | 中国科学院南京地理与湖泊研究所 | Landscape lake water replenishing purification process |
| CN112607956A (en) * | 2020-11-25 | 2021-04-06 | 中国科学院地球化学研究所 | Ecological restoration and aquatic ecosystem restoration method for polluted water body of plateau lake |
Non-Patent Citations (10)
| Title |
|---|
| ANDRZEJ PUKACZ等: "Depth-dependence andmonthly variability of charophyte biomass production: consequences for the precipitation of calcium carbonate in a shallow Chara-lake", ENVIRON SCI POLLUT RES, vol. 23, 22 August 2016 (2016-08-22), pages 22433 * |
| XU HONG等: "Biogenic carbonate formation and sedimentation in the Xisha Islands: evidences from living Halimeda", ACTA OCEANOL. SIN., vol. 34, no. 4, 17 April 2015 (2015-04-17), pages 62 - 73 * |
| 刘再华等: "云南白水台钙华水池中水化学日变化及其生物控制的发现", 水文地质工程地质, no. 6, 30 June 2005 (2005-06-30), pages 10 - 15 * |
| 刘彦;张金流;何媛媛;孙海龙;刘再华;: "单生卵囊藻对DIC的利用及其对CaCO_3沉积影响的研究", 地球化学, no. 02, 26 March 2010 (2010-03-26), pages 191 - 196 * |
| 曾振宇;晏浩;孙海龙;刘再华;: "云南白水台钙华池出入口水化学和δ~(13)C_(DIC)昼夜变化的影响因素及水生光合作用影响比例的计算", 中国岩溶, no. 06, 15 December 2016 (2016-12-15), pages 605 - 613 * |
| 李丽;蒲俊兵;李建鸿;于奭;肖琼;张陶;: "亚热带典型岩溶溪流水气界面CO_2交换通量变化过程及其环境影响", 环境科学, no. 07, 28 June 2016 (2016-06-28), pages 2487 - 2495 * |
| 李永新;: "藻类在黄龙钙华景观中的作用", 安徽农业科学, no. 09, 20 March 2011 (2011-03-20), pages 5433 - 5435 * |
| 杨明镜;李成仙;姚俊杰;沈昆根;: "喀斯特山区花溪河大型水生植物资源的初步研究", 水生态学杂志, no. 03, 15 May 2009 (2009-05-15), pages 13 - 16 * |
| 胡刚;王培;曹建华;张春来;莫碧琴;: "黑藻对岩溶水中DIC的利用及其生长的响应", 环境科学与技术, no. 05, 15 May 2016 (2016-05-15), pages 34 - 37 * |
| 黄浩;陈林;岳芯;谭铭磊;甘杰;: "四川黑水卡龙沟钙华形成影响因素", 四川地质学报, no. 04, 26 December 2017 (2017-12-26), pages 113 - 116 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhu et al. | A comparative study of iron and temperature interactive effects on diatoms and Phaeocystis antarctica from the Ross Sea, Antarctica | |
| Yoshimura et al. | Himalayan ice-core dating with snow algae | |
| Willén et al. | Summer phytoplankton in 73 nutrient-poor Swedish lakes. Classification, ordination and choice of long-term monitoring objects | |
| Fischer et al. | Stable carbon isotope composition, depth distribution and fate of macroalgae from the Antarctic Peninsula region | |
| WO2024051151A1 (en) | Silicon-releasing streptomyces cs13-6 and use thereof | |
| Bełdowska et al. | Mercury concentration in phytoplankton in response to warming of an autumn–winter season | |
| Tanaka et al. | Production of dissolved and particulate organic matter by the reef-building corals Porites cylindrica and Acropora pulchra | |
| WO2023010620A1 (en) | Biological crust restoration material for promoting ecological recovery of ionic rare earth tailings region, application, and restoration method | |
| Canfora et al. | Compartmentalization of gypsum and halite associated with cyanobacteria in saline soil crusts | |
| Douglas | Structural and geomicrobiological characteristics of a microbial community from a cold sulfide spring | |
| Rogozin et al. | Meromixis and seasonal dynamics of vertical structure of Lake Uchum (South Siberia) | |
| Jin et al. | Phosphorus utilization by phytoplankton in the Yellow Sea during spring bloom: Cell surface adsorption and intracellular accumulation | |
| Cao et al. | Advances in the research on micropropagules and their role in green tide outbreaks in the Southern Yellow Sea | |
| OULIAEI | Micromorphology of pedogenic carbonate features in soils of Kohgilouye, southwestern Iran | |
| CN115613542A (en) | Method for promoting calcium bloom restoration by submerged plants | |
| Legendre et al. | Chlorophyll a biomass and growth of sea-ice microalgae along a salinity gradient (southeastern Hudson Bay, Canadian Arctic) | |
| Panieri et al. | Microbial communities in Messinian evaporite deposits of the Vena del Gesso (northern Apennines, Italy) | |
| Kakita et al. | Effects of environmental factors and metal ions on growth of the red alga Gracilaria chorda Holmes (Gracilariales, Rhodophyta) | |
| Sundberg | The ecological significance of sexual reproduction in peat mosses (Sphagnum) | |
| CN105368756B (en) | The bacterium and application thereof of one plant of removing calcium ion and magnesium ion | |
| Jordan | Coccolithophorid communities in the North East Atlantic | |
| Liu et al. | Early Triassic stromatolites from the Xingyi area, Guizhou Province, southwest China: geobiological features and environmental implications | |
| Zhao et al. | Progress in the study of algae and mosses in biological soil crusts | |
| Dorais et al. | Assessing the potential of biochar as a growing media component for potted plants | |
| Siver et al. | History of the Giraffe Pipe locality inferred from microfossil remains: a thriving freshwater ecosystem near the Arctic Circle during the warm Eocene |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |