CN115612706A - Preparation method of soluble dietary fiber with lead-removing effect - Google Patents
Preparation method of soluble dietary fiber with lead-removing effect Download PDFInfo
- Publication number
- CN115612706A CN115612706A CN202211399325.2A CN202211399325A CN115612706A CN 115612706 A CN115612706 A CN 115612706A CN 202211399325 A CN202211399325 A CN 202211399325A CN 115612706 A CN115612706 A CN 115612706A
- Authority
- CN
- China
- Prior art keywords
- dietary fiber
- soluble dietary
- ganoderma
- culture medium
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 76
- 230000000694 effects Effects 0.000 title abstract description 11
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 238000000855 fermentation Methods 0.000 claims abstract description 45
- 230000004151 fermentation Effects 0.000 claims abstract description 45
- 241000219095 Vitis Species 0.000 claims abstract description 41
- 235000009754 Vitis X bourquina Nutrition 0.000 claims abstract description 41
- 235000012333 Vitis X labruscana Nutrition 0.000 claims abstract description 41
- 235000014787 Vitis vinifera Nutrition 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 33
- 239000001963 growth medium Substances 0.000 claims abstract description 32
- 241000222336 Ganoderma Species 0.000 claims abstract description 27
- 239000007787 solid Substances 0.000 claims abstract description 25
- 239000012535 impurity Substances 0.000 claims abstract description 21
- 238000012258 culturing Methods 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 9
- 239000001301 oxygen Substances 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 8
- 238000005360 mashing Methods 0.000 claims abstract description 8
- 239000008223 sterile water Substances 0.000 claims abstract description 8
- 230000003213 activating effect Effects 0.000 claims abstract description 7
- 238000007873 sieving Methods 0.000 claims abstract description 6
- 239000006228 supernatant Substances 0.000 claims abstract description 6
- 240000008397 Ganoderma lucidum Species 0.000 claims description 31
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims description 28
- 241001489091 Ganoderma sinense Species 0.000 claims description 15
- 241001480597 Ganoderma tsugae Species 0.000 claims description 15
- 229920005610 lignin Polymers 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 5
- 230000029142 excretion Effects 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000000813 microbial effect Effects 0.000 abstract description 5
- 238000010298 pulverizing process Methods 0.000 abstract description 4
- 238000009776 industrial production Methods 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 description 18
- 238000001179 sorption measurement Methods 0.000 description 14
- 239000000843 powder Substances 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 239000001913 cellulose Substances 0.000 description 6
- 229920002678 cellulose Polymers 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 229920001202 Inulin Polymers 0.000 description 5
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 5
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 5
- 241000194020 Streptococcus thermophilus Species 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 229910001385 heavy metal Inorganic materials 0.000 description 5
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 5
- 229940029339 inulin Drugs 0.000 description 5
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 229920002488 Hemicellulose Polymers 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 229910021645 metal ion Inorganic materials 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000013589 supplement Substances 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 239000003463 adsorbent Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- BAPJBEWLBFYGME-UHFFFAOYSA-N Methyl acrylate Chemical compound COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 150000003272 mannan oligosaccharides Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 206010027439 Metal poisoning Diseases 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 231100000206 health hazard Toxicity 0.000 description 1
- 229940059442 hemicellulase Drugs 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 208000008127 lead poisoning Diseases 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the technical field of microbial fermentation, and particularly relates to a preparation method of soluble dietary fiber with a lead-removing effect. The method comprises the following steps: raw material treatment: drying grape skin residue, removing impurities, pulverizing, and sieving; activating strains: inoculating Ganoderma into the middle part of solid culture medium from the slant with inoculating hook, and culturing at certain temperature for a certain time; preparing a seed solution: mashing the solid culture medium with Ganoderma, and adding appropriate amount of sterile water to obtain Ganoderma seed solution; fermentation culture: mixing the Ganoderma seed solution and grape skin residue at a certain ratio, placing into a fermentation tank, fermenting at a certain temperature for a certain time, and controlling the oxygen concentration in the fermentation tank within a certain range; removing impurities: removing impurities in the fermentation liquor, and collecting supernatant to obtain soluble dietary fiber solution with lead-removing effect. The method provided by the invention can greatly reduce the production cost and promote the large-scale industrial production of the soluble dietary fiber.
Description
Technical Field
The invention belongs to the technical field of microbial fermentation, and particularly relates to a preparation method of soluble dietary fiber with a lead-removing effect.
Background
Dietary Fiber (DF) refers to a vegetable food containing carbohydrates that cannot be digested and absorbed by the human body, and is listed as the "seventh nutrient" following the traditional six nutrients of carbohydrates, proteins, fats, vitamins, minerals, water. The carboxyl in the dietary fiber and the amino acid combined on the dietary fiber can exchange with harmful ions such as lead, mercury, cadmium and the like, and the absorption rate of the substances is reduced, so that toxic substances, chemicals and the like in the human body are promoted to be discharged out of the body, and the detoxification effect is achieved. Lead is a highly interesting neuro-and cytotoxicant that enters the food chain from the environment with automobile exhaust and industrial sources of pollution, and is a serious health hazard for humans, especially children. Lead poisoning has become a prominent environmental problem facing children in developing countries.
The grape skin residue is waste formed by stacking grape skin and grape seeds which are remained in the production process of products taking grapes as raw materials, and the mass of the grape skin residue is about 20% of the raw materials. According to statistics, the grape skin dregs generated by the wine brewing industry in the world are nearly thousands of tons. The current situation of domestic treatment grape skin sediment is mostly buried as fertilizer, fodder or rubbish, not only is a waste to the resource, still causes adverse effect to the environment, has also increased pressure for environmental protection work simultaneously. Therefore, the development and utilization of the grape skin slag urgently need more intensive research and discussion, the problem of environmental pollution can be relieved by reasonably utilizing the grape skin slag, the chain of the wine industry can be perfected, and the income can be improved. The total dietary fiber content in the grape skin residue can reach more than 70% of dry matter, but more than 80% of the total dietary fiber is insoluble dietary fiber with poor lead binding capacity, and the content of soluble dietary fiber with strong lead binding capacity is less.
In the prior art, the preparation of soluble dietary fiber by using grape skin residues is mainly divided into a physical method, a chemical method, an active enzyme method and a microbial fermentation method. The physical method has low yield and can not be used independently; the chemical method has more byproducts, is difficult to separate and purify and pollutes the environment; although the enzymatic method has little pollution, the cost investment is high, and the method is not beneficial to large-scale production; and by adopting a microbial fermentation method, the yield of the soluble dietary fiber is high, and most nutrients can be digested and decomposed by microorganisms in the growth process, so that the purity and the yield of the soluble dietary fiber are obviously improved. At present, the reported strains for preparing soluble dietary fibers by fermenting grape skin residues select bacteria microorganisms capable of generating lactic acid, such as lactobacillus bulgaricus, streptococcus thermophilus and the like, the requirements on nutrition are high, skim milk powder is required to be added to supplement nutrient components, the components of an initial culture medium are increased, the difficulty of later-stage separation and purification is increased, on the other hand, the catabolism capacity of bacteria on cellulose, hemicellulose and lignin is poorer than that of edible fungi, and the reported method adopts the mixed bacteria of the lactobacillus bulgaricus and the streptococcus thermophilus as fermenting microorganisms, the fermentation process is controlled complicatedly, and fermentation failure is easily caused.
Some studies have reported that, for example, in the patent with application number 201610450710.3 (title of the invention: method for preparing heavy metal adsorbent from grape skin), a heavy metal adsorbent prepared from grape skin by chemical modification method is described, but because of using various chemical reagents such as silicic acid, ammonium ceric nitrate and methyl acrylate, the heavy metal adsorbent is mainly applied in chemical field, and cannot be applied in food, medicine and cosmetic field. In the paper ' the influence of grape skin residue insoluble dietary fiber on lead content in a lead-poisoned mouse ', in food technology, 2018,43 (08): 222-226 ', the adsorption effect of insoluble dietary fiber with poor lead adsorption capacity is researched, and if the content of soluble dietary fiber is increased, the adsorption effect of the insoluble dietary fiber on lead is certainly and remarkably improved. In the thesis of 'optimization of a process for preparing grape skin residue dietary fiber by a microbial fermentation method', food science, 2012,33 (15): 178-182 'and' research on extracting soluble dietary fiber from grape skin residue by a fermentation method ', proceedings of the institute of urban construction in Henan, 2022,31 (01): 75-79+ 92' all report that the soluble dietary fiber is prepared by using a mixed bacterium of lactobacillus bulgaricus and streptococcus thermophilus, but because the lactobacillus bulgaricus and the streptococcus thermophilus have higher nutritional requirements, skim milk powder needs to be added to supplement nutritional ingredients, the composition of an initial culture medium is increased, and the difficulty of later-stage separation and purification is increased, on the other hand, the catabolic capacity of bacteria on cellulose, hemicellulose and lignin is poorer than that of edible fungi, and the reported method adopts the mixed bacterium of lactobacillus bulgaricus and streptococcus thermophilus as a fermentation microorganism, so that the fermentation process is complex to control and fermentation failure is easily caused.
Disclosure of Invention
Aiming at the technical problems, the invention provides a preparation method of soluble dietary fiber beneficial to lead removal, aiming at solving the problems of low utilization rate of grape skin residue and low yield of soluble dietary fiber with strong lead adsorption capacity in the prior art, and the soluble dietary fiber beneficial to lead removal is prepared by fermenting grape skin residue with lucid ganoderma.
The invention is realized by the following technical scheme:
a method of preparing soluble dietary fiber to aid in lead removal, the method comprising:
treating raw materials: drying grape skin residues, removing impurities, crushing and sieving;
activating strains: inoculating the slant mycelium of Ganoderma lucidum with the culture medium from the slant into the middle of the solid culture medium, and culturing at a certain temperature for a certain time;
preparing a seed solution: mashing the solid culture medium with Ganoderma, and adding appropriate amount of sterile water to obtain Ganoderma seed solution;
fermentation culture: mixing the ganoderma lucidum seed liquid and the grape skin residue according to a certain mass ratio, putting the mixture into a fermentation tank, fermenting the mixture for a certain time at a certain temperature, and controlling the oxygen concentration in the space in the fermentation tank to be within a certain range;
removing impurities: removing impurities in the fermentation liquor by filtering or centrifuging, and collecting supernatant to obtain solution containing soluble dietary fiber.
Further, the Ganoderma is Ganoderma lucidum (G.lucidum), ganoderma sinense (G.sinense), or Ganoderma tsugae (G.tsugae Murr). Three Ganoderma fungi, ganoderma lucidum (Ganoderma lucidum), ganoderma sinensis (G.sinense) and Ganoderma tsugae (G.tsugae Murr.) are included in the list of fungi for health food published by the Ministry of health in 2001. In order to ensure that the obtained product can be applied to health-care food in the future, the invention finally selects three ganoderma lucidum strains of ganoderma lucidum, ganoderma sinense and ganoderma tsugae.
Further, the strain activation specifically comprises the following steps: cutting Ganoderma with inoculating hook from inclined plane to 0.5-1cm 2 Inoculating the slant mycelium with culture medium into the middle of solid culture medium, and culturing at 28 + -2 deg.C for 3-5 days. Wherein, the solid culture medium is a commercially available potato glucose agar culture medium which accords with GB 4789.15-2016 food safety national standard food microbiology inspection mould and yeast count, 0.3-1.2% by mass of lignin is added, the lignin is added for inducing expression and secretion of a lignin degradation enzyme system in ganoderma lucidum, so that smooth degradation in the liquid fermentation process is ensured, effective degradation of the lignin is ensured, the lignin can not play a role in inducing expression and secretion of the lignin degradation enzyme system when the addition amount of the lignin is less than 0.3%, the growth of ganoderma lucidum can be inhibited when the addition amount is more than 1.2%, and subsequent liquid fermentation failure is caused.
Further, the seed liquid preparation method specifically comprises the following steps: mashing the solid culture medium with Ganoderma with inoculating hook, and adding sterile water with the amount of 2-5 times of the solid culture medium volume to obtain Ganoderma seed solution.
Further, the fermentation culture steps are specifically as follows: mixing the glossy ganoderma seed liquid and the grape skin residue according to the mass ratio of (5-10) to 1, putting the mixture into a fermentation tank, and controlling the oxygen concentration of the space in the fermentation tank to be 0.010-0.025 mol/L under the condition of 28 +/-2 ℃ for 4-10 days. Because the ganoderma lucidum belongs to aerobic microorganisms, if the supply of oxygen is insufficient, the growth of the ganoderma lucidum is slow, the decomposition of cellulose and lignin by the ganoderma lucidum is reduced, the yield of the mannan-oligosaccharide is low finally, the ganoderma lucidum is easy to be polluted by other microorganisms, and the fermentation fails, so that the fermentation condition needs to be strictly controlled in order to ensure the yield of the mannan-oligosaccharide. And in the fermentation culture process, 1.0-10.0g of yeast powder is added into the fermentation culture medium to supplement a nitrogen source.
Further, in the step of removing impurities, the mass percentage of the soluble dietary fiber in the solution containing the soluble dietary fiber is 3.00-6.60%.
Further, filtering the solution containing the soluble dietary fiber, decolorizing with active carbon, concentrating under reduced pressure at 50-70 ℃ under 0.001-0.01MPa, refining and drying to obtain soluble dietary fiber solid.
The invention has the beneficial technical effects that:
ganoderma (Ganoderma) is an important dual-purpose fungus for medicine and food in Basidiomycetes, polyporaceae and Ganoderma, and is one of traditional and rare medicinal materials in China. Three Ganoderma fungi, ganoderma lucidum (Ganoderma lucidum), ganoderma sinensis (G.sinense) and Ganoderma tsugae (G.tsugae Murr.) are included in the list of fungi for health food published by Ministry of health in 2001. During the deep fermentation process of the ganoderma, rich extracellular enzyme systems such as cellulase, hemicellulase, amylase, pectinase and the like can be generated, the rich extracellular enzyme systems ensure that the ganoderma can grow well in a substrate rich in cellulose, and the enzymes can perform various extracellular biochemical reactions outside the cells and can effectively degrade the cellulose, the hemicellulose and the like in a culture substrate. In addition, the extracellular enzyme system generated by the ganoderma lucidum can effectively degrade pectin and hemicellulose in the grape skin residues and can restrictively degrade cellulose into dietary fibers. The method is simple to operate, mild in production conditions, free of environmental pollution, low in energy consumption and capable of meeting the requirements of energy conservation and emission reduction. The soluble dietary fiber which is beneficial to lead-removing effect is produced by using the invention, so that the production cost and the environmental pollution can be greatly reduced, and the large-scale industrial production of the soluble dietary fiber is promoted.
Drawings
FIG. 1 is a flow chart of a method for preparing soluble dietary fiber for aiding lead excretion according to an embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following examples, which are intended to illustrate the present invention and are not intended to limit the scope of the present invention. It is intended that all modifications or alterations to the steps or conditions of the present invention be made without departing from the spirit or essential characteristics thereof.
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The ganoderma lucidum used in the following examples is a strain of the following species: ganoderma lucidum (G.lucidum), ganoderma sinensis (G.sinense), ganoderma tsugae (G.tsugae Murr.). Each strain was purchased from China general microbiological culture Collection center (CGMCC).
Example 1
The embodiment of the invention provides a preparation method of soluble dietary fiber for facilitating lead removal, which comprises the following steps:
(1) Raw material treatment: drying grape skin residue in an oven at 60 deg.C, removing impurities, pulverizing, and sieving with 20-40 mesh sieve.
(2) Activating strains: cutting Ganoderma lucidum (G.lucidum) with inoculating hook to 1cm from slant 2 Inoculating the slant mycelium with culture medium into the middle of solid culture medium of potato glucose agar containing 0.4 wt% lignin, and culturing at 28 + -2 deg.C for 3-5 days.
(3) Preparing a seed solution: mashing the solid culture medium with Ganoderma lucidum with inoculating hook, and adding 5 times of sterile water to obtain Ganoderma lucidum seed solution.
(4) Fermentation culture: mixing ganoderma lucidum seed liquid and grape skin residue raw material powder according to the mass ratio of 10:1, adding 1.0 percent of yeast powder by mass percentage to supplement a nitrogen source, putting the mixture into a fermentation tank, culturing for 8 days at the temperature of 28 +/-2 ℃, and controlling the oxygen concentration of the space in the fermentation tank to be 0.025mol/L.
(5) Removing impurities: removing impurities of the wall-broken homogenate by filtration or centrifugation, and collecting supernatant to obtain soluble dietary fiber solution.
The content of the soluble dietary fiber in the solution is detected to be 3.54 percent by referring to a method of GB 5009.88-2014 'determination of dietary fiber in national standard food for food safety'.
Filtering the solution containing the soluble dietary fiber, decoloring by active carbon, then decompressing and concentrating at the temperature of 50-70 ℃ under the pressure of 0.001-0.01MPa, refining and drying to obtain soluble dietary fiber solid, wherein each kilogram of grape skin residue dry powder can obtain 360.2 grams of the soluble dietary fiber solid.
Example 2
The embodiment of the invention provides a preparation method of soluble dietary fiber for helping lead removal, which comprises the following steps:
(1) Treating raw materials: drying grape skin residue in an oven at 60 deg.C, removing impurities, pulverizing, and sieving with 20 mesh sieve.
(2) Activating strains: cutting Ganoderma sinensis (G.sinense) with inoculating hook to 1cm from slant 2 Inoculating the slant mycelium with culture medium into the middle of potato glucose agar solid culture medium added with lignin with the mass percent of 1.0%, and culturing for 3-5 days at 28 +/-2 ℃.
(3) Preparing a seed solution: mashing the solid culture medium with Ganoderma sinensis with inoculating hook, and adding 5 times of sterile water to obtain Ganoderma sinensis seed solution.
(4) Fermentation culture: mixing Ganoderma sinensis seed liquid and grape skin residue raw material powder according to a mass ratio of 5:1, placing the mixture into a fermentation tank, culturing for 8 days at the temperature of 28 +/-2 ℃, and controlling the oxygen concentration of the space in the fermentation tank to be 0.025mol/L.
(5) Removing impurities: removing impurities of the wall-broken homogenate by filtration or centrifugation, and collecting supernatant to obtain soluble dietary fiber solution.
The content of the soluble dietary fiber in the solution is detected to be 6.60 percent by referring to a method of GB 5009.88-2014 determination of dietary fiber in national standard food for food safety.
Example 3
The embodiment of the invention provides a preparation method of soluble dietary fiber for facilitating lead removal, which comprises the following steps:
(1) Raw material treatment: drying grape skin residue in an oven at 60 deg.C, removing impurities, pulverizing, and sieving with 20 mesh sieve.
(2) Activating strains: cutting Ganoderma tsugae (G.tsugae Murr.) with inoculating hook 1cm from the slant 2 Inoculating the slant mycelium with culture medium into the middle of potato glucose agar solid culture medium added with 0.5% lignin by mass, and culturing at 28 + -2 deg.C for 3-5 days.
(3) Preparing a seed solution: mashing the solid culture medium with Ganoderma tsugae with an inoculation hook, and adding 5 times of sterile water to obtain Ganoderma tsugae seed solution.
(4) Fermentation culture: mixing ganoderma tsugae seed liquid and grape skin residue raw material powder according to a mass ratio of 5:1, placing the mixture into a fermentation tank, culturing for 6 days at the temperature of 28 +/-2 ℃, and controlling the oxygen concentration of the space in the fermentation tank to be 0.025mol/L.
(5) Removing impurities: removing impurities of the wall-broken homogenate by filtration or centrifugation, and collecting supernatant to obtain soluble dietary fiber solution.
The content of the soluble dietary fiber in the solution is detected to be 5.13% by referring to a method of GB 5009.88-2014 'determination of dietary fiber in national standard food for food safety'.
Comparative example
The grape skin residue is dried in an oven at 60 ℃, impurities are removed, then the grape skin residue is crushed and sieved by a 20-mesh sieve, and the total dietary fiber content is detected to be 66.1 percent by referring to the method of GB 5009.88-2014 'determination of dietary fiber in national standard food for food safety', wherein the insoluble dietary fiber content is 54.9 percent, and the soluble dietary fiber content is 11.2 percent. According to the following steps of 10:1 and 5:1, the soluble dietary fibre content was 1.02% and 1.87% respectively after mixing with water. In examples 1-3, the soluble dietary fiber content was 3.54%, 6.60% and 5.13%, respectively, which was significantly higher than that of the unfermented grape skin residue.
Measurement of adsorption Capacity for heavy Metal
Preparing a lead metal ion solution with the concentration of 1.0g/L, accurately weighing a soluble dietary fiber sample (the soluble dietary fiber solid obtained in the embodiment 1) and a grape skin residue sample which is not fermented by lucid ganoderma by 1.0g respectively, and selecting commercially available inulin (also called inulin, which is high-quality soluble dietary fiber and is confirmed as a well-known safe substance by the Food and Drug Administration (FDA) and approved as new resource food by China. (2009 No. 5 of 2009, ministry of health) as a positive control, accurately weighing 1.0g of inulin, adding 100mL of 1.0g/L metal solution, adjusting the pH value to 7.0, oscillating at 37 ℃ for 3h, centrifuging at 4000r/min for 20min, measuring the content of heavy metal in the solution by using an atomic absorption spectrophotometer, and calculating the adsorption capacity according to the content difference before and after the reaction. The mean was taken in triplicate for each sample.
Metal ion adsorption amount (mg/g) = m 1 －m 2 /m
In the formula: m is a unit of 1 The content (mg) of metal ions before adsorption; m is 2 Is the metal ion content (mg) after adsorption; m is the mass of the dietary fiber per gram.
Detecting Pb of soluble dietary fiber prepared from Ganoderma fermented grape skin residue + The adsorption amount of (a) is 67.8 +/-3.1 mg/g, and Pb of the grape skin residue sample which is not fermented by the lucid ganoderma + The adsorption amount of (b) is 7.4 +/-1.2 mg/g, pb of inulin + The adsorption amount of (b) is 50.8. + -. 3.0mg/g.
Therefore, the lead adsorption capacity of the soluble dietary fiber prepared by fermenting the grape skin residue with the lucid ganoderma is obviously improved and is superior to that of the commercial inulin Pb + The adsorption capacity of (1).
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. A method for preparing soluble dietary fiber for aiding lead excretion, the method comprising:
treating raw materials: drying grape skin residues, removing impurities, crushing and sieving;
activating strains: inoculating the slant mycelium of Ganoderma lucidum with the culture medium cut from the slant into the middle part of the solid culture medium, and culturing at a certain temperature for a certain time;
preparing a seed solution: mashing the solid culture medium with the ganoderma lucidum, and then adding a proper amount of sterile water to obtain ganoderma lucidum seed liquid;
fermentation culture: mixing the ganoderma lucidum seed liquid and the grape skin residue according to a certain mass ratio, putting the mixture into a fermentation tank, fermenting the mixture for a certain time at a certain temperature, and controlling the oxygen concentration of the space in the fermentation tank within a certain range;
removing impurities: removing impurities in the fermentation liquor by filtering or centrifuging, and collecting supernatant to obtain solution containing soluble dietary fiber.
2. The method of claim 1, wherein the Ganoderma lucidum is selected from Ganoderma lucidum, ganoderma sinensis, and Ganoderma tsugae.
3. The method for preparing the soluble dietary fiber for helping lead excretion according to claim 1, wherein the step of activating the strain comprises the following steps: cutting Ganoderma with inoculating hook from inclined plane to 0.5-1cm 2 Inoculating the slant mycelia with the culture medium into the middle part of a solid culture medium, and culturing at 28 +/-2 ℃ for 3-5 days, wherein the solid culture medium is a potato glucose agar culture medium added with 0.3-1.2% of lignin by mass percent.
4. The method for preparing the soluble dietary fiber for helping lead removal according to claim 1, wherein the seed solution is prepared by the following steps: mashing the solid culture medium with Ganoderma with inoculating hook, and adding sterile water with the volume of 2-5 times of the solid culture medium to obtain Ganoderma seed solution.
5. The method for preparing the soluble dietary fiber for helping lead excretion according to claim 1, wherein the fermentation culture comprises the following steps: mixing the glossy ganoderma seed liquid and the grape skin residue according to the mass ratio of (5-10) to 1, putting the mixture into a fermentation tank, and controlling the oxygen concentration of the space in the fermentation tank to be 0.010-0.025 mol/L under the condition of 28 +/-2 ℃ for 4-10 days.
6. The method for preparing soluble dietary fiber for helping lead excretion according to claim 1, wherein the soluble dietary fiber content of the solution containing the soluble dietary fiber in the impurity removing step is 3.00-6.60% by weight.
7. The method for preparing soluble dietary fiber helpful for lead removal as claimed in claim 1, wherein the solution containing soluble dietary fiber is filtered and decolorized with activated carbon, then concentrated under reduced pressure at 0.001-0.01MPa and 50-70 ℃, refined and dried to obtain soluble dietary fiber solid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211399325.2A CN115612706A (en) | 2022-11-09 | 2022-11-09 | Preparation method of soluble dietary fiber with lead-removing effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211399325.2A CN115612706A (en) | 2022-11-09 | 2022-11-09 | Preparation method of soluble dietary fiber with lead-removing effect |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115612706A true CN115612706A (en) | 2023-01-17 |
Family
ID=84879317
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211399325.2A Pending CN115612706A (en) | 2022-11-09 | 2022-11-09 | Preparation method of soluble dietary fiber with lead-removing effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115612706A (en) |
-
2022
- 2022-11-09 CN CN202211399325.2A patent/CN115612706A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102268490B (en) | Clean technique for co-producing xylose, xylitol and arabinose from agricultural waste and forest waste | |
| CN101691538B (en) | Aspergillus oryzae and method for preparing high purity galacto-oligosaccharides by using same | |
| CN102796673A (en) | Feruloyl esterase production strain and method for producing feruloyl esterase by using same | |
| CN114289446A (en) | Resourceful treatment method for kitchen waste | |
| CN106636230A (en) | Method for producing lactic acid through combined fermentation of kitchen waste having undergone enzymatic hydrolysis and corn stalk | |
| CN110093281B (en) | Phellinus igniarius liquid submerged fermentation culture process | |
| CN112011531A (en) | Enzyme preparation produced by using immobilized cell technology and preparation method thereof | |
| CN113621674B (en) | Method for producing L-lactic acid by using liquor distiller grains | |
| CN114107403B (en) | Method for co-producing ellagic acid and biological feed by fermenting pericarpium Granati with microbial community | |
| CN113321548B (en) | Organic fertilizer prepared by comprehensively utilizing wastes generated in beer production and preparation method thereof | |
| CN108251476B (en) | Method for extracting vitamin B12 from enzyme preparation wastewater | |
| CN102392008B (en) | Bioprotein capable of replacing protein raw material and preparation method of bioprotein | |
| CN111019996A (en) | Method for preparing active polypeptide by liquid fermentation of camellia seed meal | |
| CN102173879B (en) | Method for producing biological potassium fertilizer by utilizing cellulose fermented waste mycelium and biogas residue | |
| CN101654697A (en) | Method for preparing rapeseed peptides by mixed fermentation | |
| CN113957109B (en) | Industrial green production process of polystictus glycopeptide | |
| CN115612706A (en) | Preparation method of soluble dietary fiber with lead-removing effect | |
| CN113416757B (en) | Method for preparing epigallocatechin through biological fermentation | |
| CN102643754B (en) | Aspergillus oryzae and application thereof in aspect of improving yield of alcohol | |
| CN102212480A (en) | Method for producing yeast extract by utilizing tetracycline decoction dregs | |
| CN110583867B (en) | Method for producing protein feed from waste potato residues in potato starch processing | |
| CN110885760B (en) | Liquid fermentation method for producing pectinase | |
| CN110791436B (en) | Aspergillus niger strain capable of producing pectinase at high yield and application thereof | |
| CN110964706A (en) | Preparation method of cellulase, xylanase and pectinase | |
| CN110938552A (en) | Method for degrading herb tea residue and preparing aspergillus niger |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |