CN115595289A - Autologous cell suspension for promoting hair regeneration and preparation method and application thereof - Google Patents

Autologous cell suspension for promoting hair regeneration and preparation method and application thereof Download PDF

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Publication number
CN115595289A
CN115595289A CN202110720869.3A CN202110720869A CN115595289A CN 115595289 A CN115595289 A CN 115595289A CN 202110720869 A CN202110720869 A CN 202110720869A CN 115595289 A CN115595289 A CN 115595289A
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cell suspension
decomposition
autologous cell
preparation
autologous
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叶文珏
程庆伟
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Beijing Weili New Century Science And Technology Development Ltd By Share Ltd
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Beijing Weili New Century Science And Technology Development Ltd By Share Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation

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  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • General Engineering & Computer Science (AREA)
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Abstract

The invention discloses an autologous cell suspension for promoting hair regeneration and a preparation method and application thereof, belonging to the technical field of biological medicines. The autologous cell suspension for promoting hair regeneration is obtained by decomposing skin tissue with hair follicles by using a cell suspension preparation device, wherein the autologous cell suspension is derived from the whole layer of the skin tissue with hair follicles in the occiput on the head side of a patient. The invention belongs to the homologous cell transplantation technology, and the skin tissue of the occiput of the head of a patient is decomposed into cell suspension and then is used for the alopecia part of the patient. The autologous cell suspension is prepared by adopting the cell suspension preparation device, has simple process and convenient operation, is used for the alopecia part of a patient, can arouse sleeping hair and promote the natural growth of the hair, and has obvious effect.

Description

Autologous cell suspension for promoting hair regeneration and preparation method and application thereof
Technical Field
The invention relates to the technical field of biomedicine, in particular to autologous cell suspension for promoting hair regeneration and a preparation method and application thereof.
Background
Alopecia has been a long-standing problem, and the treatment methods for treating alopecia in the current market mainly comprise: hair washing, hair care and hair nourishing, oral administration or medicine application, traditional Chinese medicine conditioning or surgical hair transplantation. Wherein, the hair washing, hair care and hair nourishing have slow effect and poor effect; oral administration or smearing medicine and traditional Chinese medicine conditioning often have the problem that the medicine cannot be taken consistently, inconvenience is brought to life of patients, and the patients can relapse after stopping taking the medicine, continue to suffer from alopecia and even suffer from adverse reactions of sexual dysfunction. Surgical hair transplantation techniques include follicular unit scalp strip cutting techniques and follicular unit extraction techniques. The method has the disadvantages of complicated operation, long time, slow recovery, and long time for patients to take rest, which affects life and work. Moreover, the survival hair can not grow any more with the probability of 30-50 percent, and the effect can not reach the satisfaction of patients.
It is therefore apparent that the above-mentioned conventional methods for treating alopecia still have the disadvantages and drawbacks, and further improvements are desired. How to create a new autologous cell suspension for promoting hair regeneration and a preparation method and application thereof, so that autologous cells are utilized to promote autologous hair growth, the problem of alopecia of patients is fundamentally solved, the operation is simple, the recovery is fast, and the method becomes an extremely improved target in the current industry.
Disclosure of Invention
The invention aims to provide autologous cell suspension for promoting hair regeneration, which utilizes autologous cells to promote autologous hair growth and is used for fundamentally solving the problem of alopecia of patients.
In order to solve the above-mentioned problems, the present invention provides an autologous cell suspension for promoting hair regeneration, which is derived from a skin tissue with hair follicles in the whole layer of the occipital region on the head of a patient and is obtained by cell dissociation of the skin tissue.
The autologous cell suspension is obtained by decomposing the skin tissue by using a cell suspension preparation device.
The aperture of the micropore of the decomposition filter screen in the cell suspension preparation device is 85-100 mu m, the micropore of the decomposition filter screen is formed by punching a polygonal conical punch, and the inner side of each micropore is provided with a plurality of sharp edges facing the inner part of the decomposition chamber.
The preparation method of the autologous cell suspension comprises the following steps:
(1) Placing skin tissue of occiput of patient head side into decomposition chamber of cell suspension preparation device, and fastening protective cover body; meanwhile, 1.2ml of normal saline is injected into the collection chamber through an extraction mechanism of the cell suspension preparation device, and a sealing cover body is tightly buckled;
(2) Starting a power mechanism of the cell suspension preparation device to enable a rotating shaft of the cell suspension preparation device to rotate at the rotating speed of 100rpm twice for 1min each time, then extracting 1ml of decomposition liquid in a collection chamber from an extraction channel, injecting 1.2ml of physiological saline, continuously starting the rotating shaft to enable the rotating shaft to rotate for 1min to complete decomposition, extracting the decomposition liquid in the decomposition chamber, and mixing the decomposition liquid with the decomposition liquid extracted in the early stage to obtain the autologous cell suspension for promoting hair regeneration.
The autologous cell suspension is prepared within 10min by the preparation method.
In another embodiment of the present invention, there is also provided a method for preparing the autologous cell suspension for promoting hair regeneration, including the steps of:
(1) Placing skin tissue of occiput at head side of patient into decomposition chamber of cell suspension preparation device, and fastening protective cover body; meanwhile, 1.2ml of normal saline is injected into the collection chamber through an extraction mechanism of the cell suspension preparation device, and a sealing cover body is tightly buckled;
(2) Starting a power mechanism of the cell suspension preparation device to enable a rotating shaft of the cell suspension preparation device to rotate at the rotating speed of 100rpm twice for 1min each time, then extracting 1ml of decomposition liquid in a collection chamber from an extraction channel, injecting 1.2ml of physiological saline, continuously starting the rotating shaft to enable the rotating shaft to rotate for 1min to complete decomposition, extracting the decomposition liquid in the decomposition chamber, and mixing the decomposition liquid with the decomposition liquid extracted in the early stage to obtain the autologous cell suspension for promoting hair regeneration.
The preparation method is controlled to be completed within 10 min.
According to another embodiment of the invention, the application of the autologous cell suspension in preparing a preparation for promoting hair regeneration is also provided.
The autologous cell suspension is injected at the bald tissue.
The autologous cell suspension is injected to the alopecia tissue of the patient from the skin tissue of the occiput on the head side of the patient, and the time is controlled within 15 min.
After adopting such design, the invention has at least the following advantages:
the autologous cell suspension for promoting hair regeneration is used for the alopecia part of a patient because the cells of the autologous cell suspension originate from the skin tissue of the occiput of the head side of the patient, belongs to the homologous cell transplantation technology, only a small amount of physiological saline is added in the preparation process of the cell suspension, no additive is added, and when the cell suspension is injected into the sparse hair part, the cell suspension can wake up the sleeping hair and promote the natural growth of the hair, has remarkable effect and completely solves the problem of alopecia. And the whole cell suspension preparation process is simple to operate, the damage area to the patient is small, the normal working life of the patient is not influenced, and the compliance of the patient is good.
Detailed Description
The disclosure in the invention application 201911063274.4 is incorporated by reference in the present application. The cell suspension preparation device adopted in the application is the cell suspension preparation device disclosed in the invention application 201911063274.4. The cell suspension preparation device is used for decomposing biological tissues to form cell suspensions.
The cell suspension preparation apparatus includes: the decomposition chamber is of a cylindrical structure with a hollow interior, and the inner wall of the cylindrical structure is of a step-shaped structure; the decomposition mechanism comprises a decomposition filter screen, a spiral blade, a rotating shaft and a power mechanism, wherein the spiral blade is arranged on the upper surface of the decomposition filter screen, the rotating shaft and the power mechanism drive the spiral blade to rotate; the collection chamber is arranged at the lower part of the cylindrical structure of the decomposition chamber and is used for receiving the cell suspension filtered by the decomposition filter screen; wherein, the collection room is the collection container of taking the mechanism, and the upper portion and the tubular structure lower part zonulae occludens of collection container draw the mechanism to set up on collection container's lateral wall, and draw including at least one in the mechanism and draw the passageway, draw the one end of passageway and collect the inside of container and communicate with each other, the other end communicates with each other with collection container's outside, and draws the outer port department of passageway and be equipped with sealed lid.
Examples of the preparation of autologous cell suspensions that promote hair regrowth using this cell suspension preparation device of the present application are as follows.
Several 1 x 2.5mm full-layer skin tissues with hair follicles on the occiput of the head side of the patient were drilled using a 2.5mm disposable microscopic skin biopsy loop, and were rapidly placed into the decomposition chamber of the cell suspension preparation device, and simultaneously, 1.2ml of physiological saline was injected into the collection chamber through the extraction mechanism of the cell suspension preparation device, and the closure body was tightly closed. Starting the power mechanism of the cell suspension preparation device to crack the skin tissue.
The working parameters of the cell suspension preparation device are of vital importance, and the quality of the obtained cell suspension and the application effect of the cell suspension are directly influenced.
Specifically, the cell suspension preparation apparatus has a decomposition screen having micropores punched by a polygonal conical punch, each micropore having a plurality of sharp edges on the inside facing the inside of the decomposition chamber. In the embodiment, 100-120 regular hexagon micropores are uniformly distributed on the decomposition filter screen, namely, the blades are formed by 600-720 sharp edges, and the pore size of each micropore is 85-100 mu m. It should be noted that if the pore size is too large, the skin tissue will be present in the cell suspension as small pieces of tissue, and the desired cells cannot be lysed, so that the effective cell concentration is not high; if the pore size is too small, the tissue lysis effect is poor, and the effective cell concentration in the formed cell suspension is still not high, so that the selection of an appropriate pore size has a large influence on the quality of the obtained cell suspension, and further influences on the subsequent application of the cell suspension.
In addition, the cell suspension preparation device comprises the following specific operation steps:
starting a power mechanism of the cell suspension preparation device to rotate a rotating shaft of the cell suspension preparation device at the rotating speed of 100rpm twice for 1min each time, then extracting 1ml of decomposition liquid in a collection chamber from an extraction channel, injecting 1.2ml of physiological saline, continuously starting the rotating shaft to rotate the rotating shaft for 1min to complete decomposition, extracting the decomposition liquid in the decomposition chamber, and mixing the decomposition liquid with the decomposition liquid extracted in the early stage to obtain the autologous cell suspension for promoting hair regeneration.
It should be noted that, when the rotating speed of the rotating shaft is too fast or too slow during the skin tissue decomposition process, the tissue cells are damaged, and the activity of the cells is affected. And the autologous cell suspension is prepared within 10min from the step of obtaining the skin tissue to the step of preparing the cell suspension, so that the biological activity of the cells is ensured.
The cell suspension prepared by the preparation method is applied to a preparation for promoting hair regeneration, and specific examples are as follows.
The cell suspension prepared above was introduced into a 1ml screw syringe by a converter, and injected into the site of alopecia on the head of the homologous patient using a 30G sharp needle. And allowing the autologous cell suspension to complete within 15min from the time of obtaining the homologous skin tissue to the time of completing the cell suspension injection.
The hair loss problem of the patients is treated by adopting the autologous cell transplantation method, and the applicant treats 317 patients, and finds that 95 percent of hairs of the patients begin to grow within 1 month after injection, the newly grown hairs are healthy, strong, elastic and good in glossiness, white hairs can be changed into black hairs, the hairs of the original hair loss part are dense after 3 months, the obvious improvement is achieved, and the problem of hair re-loss of the hairs of the original hair loss part is found through follow-up of the patients.
The application of the autologous cell suspension is suitable for people suffering from initial and middle alopecia, and has a remarkable curative effect of obviously improving alopecia. Is also suitable for the population of hair planting combination therapy, can improve the microenvironment of the scalp, establish new blood circulation, achieve better prevention of alopecia reoccurrence and ensure that the hair planted is more active and healthy. And the wound of the occiput of the head side caused by the application only needs to be pasted with a regeneration paste, does not need to be sewed, does not need special care, is naturally healed, and does not influence the work and life.
While the invention has been described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.

Claims (10)

1. An autologous cell suspension for promoting hair regeneration, which is derived from a full-thickness skin tissue with hair follicles in the occiput of the head side of a patient and is obtained by decomposing cells of the skin tissue.
2. The autologous cell suspension according to claim 1, wherein the autologous cell suspension is obtained by subjecting the skin tissue to a decomposition treatment using a cell suspension preparation apparatus.
3. The autologous cell suspension of claim 2, wherein the size of the pores of the decomposition screen in the cell suspension preparation device is 85-100 μm, and the pores of the decomposition screen are punched by a polygonal conical punch and each of the pores has a plurality of sharp edges facing the inside of the decomposition chamber.
4. The autologous cell suspension of claim 3, wherein the preparation method of the autologous cell suspension comprises the following steps:
(1) Placing skin tissue of occiput at head side of patient into decomposition chamber of cell suspension preparation device, and fastening protective cover body; meanwhile, 1.2ml of normal saline is injected into the collection chamber through an extraction mechanism of the cell suspension preparation device, and a sealing cover body is tightly buckled;
(2) Starting a power mechanism of the cell suspension preparation device to enable a rotating shaft of the cell suspension preparation device to rotate at the rotating speed of 100rpm twice for 1min each time, then extracting 1ml of decomposition liquid in a collection chamber from an extraction channel, injecting 1.2ml of physiological saline, continuously starting the rotating shaft to enable the rotating shaft to rotate for 1min to complete decomposition, extracting the decomposition liquid in the decomposition chamber, and mixing the decomposition liquid with the decomposition liquid extracted in the early stage to obtain the autologous cell suspension for promoting hair regeneration.
5. The autologous cell suspension of claim 4, wherein the autologous cell suspension is prepared within 10min by the preparation method.
6. The method for preparing an autologous cell suspension for promoting hair regeneration according to any one of claims 1 to 5, wherein the preparation method comprises the following steps:
(1) Placing skin tissue of occiput at head side of patient into decomposition chamber of cell suspension preparation device, and fastening protective cover body; meanwhile, 1.2ml of normal saline is injected into the collection chamber through an extraction mechanism of the cell suspension preparation device, and a sealing cover body is tightly buckled;
(2) Starting a power mechanism of the cell suspension preparation device to enable a rotating shaft of the cell suspension preparation device to rotate at the rotating speed of 100rpm twice for 1min each time, then extracting 1ml of decomposition liquid in a collection chamber from an extraction channel, injecting 1.2ml of physiological saline, continuously starting the rotating shaft to enable the rotating shaft to rotate for 1min to complete decomposition, extracting the decomposition liquid in the decomposition chamber, and mixing the decomposition liquid with the decomposition liquid extracted in the early stage to obtain the autologous cell suspension for promoting hair regeneration.
7. The method for preparing autologous cell suspension according to claim 6, wherein the preparation is completed within 10 min.
8. Use of the autologous cell suspension of any one of claims 1 to 5 for the preparation of a preparation for promoting hair regrowth.
9. Use of the autologous cell suspension according to claim 8 for preparing a preparation for promoting hair regeneration, wherein the autologous cell suspension is injected at the site of hair loss tissue.
10. Use of autologous cell suspension according to claim 9 for preparing a preparation for promoting hair regeneration, wherein the autologous cell suspension is administered within 15min from the time of obtaining the skin tissue of the occipital region on the head side of the patient to the time of injection at the hair loss tissue of the patient.
CN202110720869.3A 2021-06-28 2021-06-28 Autologous cell suspension for promoting hair regeneration and preparation method and application thereof Pending CN115595289A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110720869.3A CN115595289A (en) 2021-06-28 2021-06-28 Autologous cell suspension for promoting hair regeneration and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110720869.3A CN115595289A (en) 2021-06-28 2021-06-28 Autologous cell suspension for promoting hair regeneration and preparation method and application thereof

Publications (1)

Publication Number Publication Date
CN115595289A true CN115595289A (en) 2023-01-13

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Country Status (1)

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CN (1) CN115595289A (en)

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