CN115581732A - Application of palladium nano material in preparation of antibacterial drugs - Google Patents
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Abstract
The invention belongs to the technical field of antibacterial materials, and discloses an application of a palladium nano material in preparation of antibacterial drugs, wherein polyvinylpyrrolidone, ascorbic acid and citric acid are uniformly stirred, and a sodium chloropalladate solution, titanium dioxide and 3, 4-difluorothiophenol are added to obtain the palladium nano material; extracting green tea with alcohol, acetylating polyphenol, separating, hydrolyzing, and drying to obtain epigallocatechin gallate; respectively weighing fructus forsythiae and fructus Gardeniae, mixing, reflux-extracting with ethanol, concentrating under reduced pressure, adsorbing, removing impurities, and drying under reduced pressure to obtain extract; dissolving the raw materials for preparing the medicament, polylactic acid and polycaprolactone into an organic solvent for electrostatic spinning, volatilizing the organic solvent, and drying to obtain the antibacterial medicament based on the palladium nano material. The preparation method of the antibacterial drug based on the palladium nano material is simple and easy to operate, the cost is low, and the prepared antibacterial drug has a good antibacterial effect on candida albicans, escherichia coli and staphylococcus aureus.
Description
Technical Field
The invention belongs to the technical field of antibacterial materials, and particularly relates to an application of a palladium nano material in preparation of antibacterial drugs.
Background
At present, infectious diseases caused by bacteria seriously threaten human health, particularly, the appearance of super bacteria arouses more and more attention of people on microorganisms which harm human survival, so that the research and development of various materials with antibacterial and bactericidal properties have extremely important significance for protecting human beings, reducing diseases and creating green and healthy environments, and the development of antibacterial materials also forms a huge and wide market.
With the development of nanotechnology and nanoscience, the nano material has become a hot research point of the antibacterial material due to its large specific surface area, excellent antibacterial performance and no drug resistance. Among them, silver nano materials are the most widely studied, but they have high toxicity and poor antibacterial durability and stability. The prior art (Advanced Materials,2015,27 (6): 1097-1104) discloses a preparation method of a nano composite material of porous silica loaded with gold particles, wherein the gold particles are loaded into the pore channels of the porous silica by the antibacterial agent, and the antibacterial agent has higher antibacterial performance and antibacterial stability by promoting the generation of active oxygen in bacteria. However, the antibacterial agent needs to synthesize porous silica in advance, which increases the complexity of the preparation process; and only gold nanoparticles with low oxidase and peroxidase activities in the precious metal nanometer material are loaded into the silicon dioxide pore channels, so that the activity of the nanometer enzyme can be only partially improved, the activity of part of the enzyme is limited, and the antibacterial effect is finally influenced. Therefore, there is a need to design a new palladium nanomaterial and its application in preparing antibacterial drugs.
Through the above analysis, the problems and defects of the prior art are as follows:
(1) The existing silver nano material has high toxicity and poor antibacterial durability and stability.
(2) The existing antibacterial agent needs to synthesize porous silicon dioxide in advance, so that the complexity of the preparation process is increased; and only gold nanoparticles with low oxidase and peroxidase activities are loaded in the silicon dioxide pore channels, so that the activity of the nanoparticles can be only partially improved, the activity of part of enzymes is limited, and the antibacterial effect is finally influenced.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an application of a palladium nano material in preparing an antibacterial drug.
The invention is realized in such a way that the preparation method of the antibacterial drug based on the palladium nano material comprises the following steps:
step one, preparing a palladium nano material: uniformly stirring and mixing a polyvinylpyrrolidone solution, an ascorbic acid solution and a citric acid solution according to a certain volume ratio; adding a sodium chloropalladate solution to react for a period of time under a certain condition, and then adding titanium dioxide and 3, 4-difluorothiophenol to obtain a palladium nano material;
step two, preparing epigallocatechin gallate: extracting green tea with alcohol to obtain crude green tea extract; acetylating polyphenol substance hydroxyl in green tea extractive crude liquid, separating, hydrolyzing in protonic solvent under acidic condition, and drying to obtain epigallocatechin gallate;
step three, preparing the forsythia suspense extract and the gardenia jasminoides extract: respectively weighing fructus forsythiae and fructus Gardeniae, mixing, extracting with ethanol under reflux, concentrating the extractive solution under reduced pressure, and dispersing in water to obtain a sample solution; adsorbing the sample solution with low-polarity or non-polar macroporous adsorbent resin, removing impurities, and drying under reduced pressure to obtain extract;
step four, preparing the antibacterial drug: dissolving the raw materials for preparing the medicament, polylactic acid and polycaprolactone in an organic solvent to obtain high polymer electrospinning solution; and (3) carrying out electrostatic spinning on the high polymer electrospinning solution under a certain condition, volatilizing the organic solvent, and drying in the dark to prepare the antibacterial drug based on the palladium nano material.
Further, the preparation method of the palladium nano-material in the first step comprises the following steps:
(1) Sequentially preparing a polyvinylpyrrolidone solution, an ascorbic acid solution and a citric acid solution, sequentially placing the polyvinylpyrrolidone solution, the ascorbic acid solution and the citric acid solution in a stirring device according to the volume ratio of 1-3;
(2) Adding a sodium chloropalladate solution into the mixed solution obtained in the step (1), placing the mixed solution into a reaction device, uniformly stirring and mixing, and reacting for 4-6 hours at the temperature of 90-110 ℃ to obtain a palladium nano material solution;
(3) And (3) adding titanium dioxide serving as a carrier into the palladium nano material solution obtained in the step (2), adding 3, 4-difluorothiophenol, and stirring to obtain the palladium nano material with the size of 10-15 nm.
Further, the preparation method of epigallocatechin gallate in the second step comprises the following steps:
(1) Placing a green tea medicinal material into an extraction device, heating and refluxing the green tea medicinal material by using 60% ethanol with the weight being 10-15 times that of the green tea medicinal material for 2-3 times, wherein each time lasts for 2-4 hours, and combining the respectively obtained extracting solutions to obtain a green tea extracting crude solution;
(2) Acetylating polyphenol substance hydroxyl in the green tea extract crude liquid by using an acetylating reagent, and separating by using silica gel column chromatography to obtain fully acetylated epigallocatechin gallate;
(3) Hydrolyzing the acetylated epigallocatechin gallate in a protic solvent under acidic conditions to obtain epigallocatechin gallate, and drying to obtain epigallocatechin gallate.
Further, the preparation method of the forsythia suspense extract and the gardenia jasminoides ellis extract in the third step comprises the following steps:
(1) Weighing fructus forsythiae and fructus gardeniae according to the mass parts, mixing, placing in an extraction device, and extracting the mixed material by refluxing with 30-80% ethanol in an amount which is 8-12 times the mass parts for 2-4 times, wherein each time for 1-3 hours;
(2) Cooling and centrifuging the extracting solution obtained in the step (1), filtering until filtrate is clear, combining, concentrating under reduced pressure to obtain clear paste with the relative density of 1.25-1.45 g/mL, and adding sterilized deionized water for dispersion to obtain a sample solution;
(3) And (3) adsorbing the sample solution obtained in the step (2) by using weak-polarity or non-polarity macroporous adsorption resin, eluting the volume of the resin with water to remove impurities, collecting ethanol eluate, and drying under reduced pressure to obtain the extract.
Further, the preparation method of the antibacterial agent in the fourth step comprises the following steps:
(1) Dissolving a palladium nano material, a fructus forsythiae extract, a fructus gardeniae extract, epigallocatechin gallate, curcumin, a pleiomycin antibacterial compound, polylactic acid and polycaprolactone into an organic solvent to obtain a high polymer electrospinning solution, wherein the organic solvent is a mixed solution consisting of ethanol and acetone in a volume ratio of 2;
(2) Under the conditions of 25-35 ℃ and 45-65% relative humidity, applying 10-25 kV voltage to the high polymer electrospinning solution, and carrying out electrostatic spinning at the advancing speed of 0.3-0.6 mL/min;
(3) Volatilizing an organic solvent from the electrostatic spinning reaction product obtained in the step (2), and naturally drying under a dark condition to prepare the antibacterial drug based on the palladium nano material.
The invention also aims to provide the antibacterial drug based on the palladium nano material, which is prepared by the preparation method of the antibacterial drug based on the palladium nano material, wherein the antibacterial drug based on the palladium nano material comprises, by mass, 30-50 parts of the palladium nano material, 20-30 parts of a forsythia suspense extract, 15-20 parts of a gardenia extract, 10-15 parts of epigallocatechin gallate, 8-12 parts of curcumin, 6-8 parts of pleconycin antibacterial complex and the balance of a drug additive;
wherein the pharmaceutical additive is selected from one or more of magnesium carbonate, magnesium stearate, starch, talc, cyclodextrin, filler, disintegrant, lubricant or emulsifier.
Another object of the present invention is to provide a system for preparing an antibacterial agent based on a palladium nanomaterial, in which the method for preparing an antibacterial agent based on a palladium nanomaterial is applied, the system comprising:
the palladium nano material preparation module is connected with the central control module and is used for sequentially placing polyvinylpyrrolidone, ascorbic acid and citric acid in the reaction device to prepare the palladium nano material;
the green tea crude extract preparation module is connected with the central control module and is used for placing a green tea medicinal material in an extraction device, heating, refluxing and extracting the green tea medicinal material by using ethanol for 2-3 times, and combining the extracting solution to obtain a green tea crude extract;
an epigallocatechin gallate preparation module connected with the central control module and used for acetylating hydroxyl in the green tea extract crude liquid, separating and hydrolyzing, and drying to obtain epigallocatechin gallate;
the extract preparation module is connected with the central control module and is used for mixing fructus forsythiae and fructus gardeniae, placing the mixture into an extraction device, performing reflux extraction by using ethanol, and performing adsorption and impurity removal by using macroporous adsorption resin to obtain an extract;
the central control module is connected with each module of the preparation system and is used for coordinating and controlling the normal operation of each module of the preparation system of the antibacterial drug based on the palladium nano material through the central processing unit;
and the antibacterial drug preparation module is connected with the central control module and is used for dissolving the drug preparation raw materials, the polylactic acid and the polycaprolactone into an organic solvent, carrying out electrostatic spinning under a certain condition, volatilizing the organic solvent, and naturally drying under a light-shading condition to prepare the antibacterial drug based on the palladium nano material.
Another object of the present invention is to provide a computer program product stored on a computer readable medium, which includes a computer readable program for providing a user input interface to apply the steps of the method for preparing an antibacterial agent based on palladium nanomaterial when the computer program product is executed on an electronic device.
Another object of the present invention is to provide a computer-readable storage medium storing instructions which, when executed on a computer, cause the computer to apply the steps of the method for preparing an antibacterial agent based on a palladium nanomaterial.
Another object of the present invention is to provide an information data processing terminal for implementing the system for preparing an antibacterial agent based on a palladium nanomaterial.
In combination with the technical solutions and the technical problems to be solved, please analyze the advantages and positive effects of the technical solutions to be protected in the present invention from the following aspects:
first, aiming at the technical problems existing in the prior art and the difficulty in solving the problems, the technical problems to be solved by the technical scheme of the present invention are closely combined with results, data and the like in the research and development process, and some creative technical effects are brought after the problems are solved. The specific description is as follows:
in the antibacterial drug based on the palladium nano material, the palladium nano material plays the roles of oxidase and peroxidase to achieve the antibacterial effect, but not release ions; through unique surface modification, the structure and the surface interface performance of the palladium nano material are changed, and a brand-new palladium nano material modified by mercaptan is obtained, so that the palladium nano material has a stable and lasting antibacterial effect. The invention takes related terpenoid components and phenolic components in forsythia and gardenia as basic components of the drug substance, and adopts macroporous adsorption resin to separate and enrich the drug substance to obtain the drug substance with antibacterial, synergistic and antibacterial effects; wherein fructus forsythiae extract and fructus Gardeniae extract have inhibitory effect on various pathogenic microorganisms, such as antibacterial, antifungal, antiviral, and leptospira killing effects.
In addition, the antibacterial drug loaded with the palladium nano material, the epigallocatechin gallate, the curcumin, the forsythia suspensa extract, the gardenia extract and the pleconycin antibacterial compound is prepared from the antibacterial drug and the biodegradable high polymer material by an electrospinning technology, and the antibacterial drug has good biocompatibility and degradability, so that the internal drug can be released continuously and slowly, and the aim of effective antibiosis is fulfilled.
The invention utilizes the mutual synergistic effect of the antibiotic composition of the pleiomycin, the palladium nano material, the epigallocatechin gallate, the curcumin, the forsythia extract and the gardenia extract to attack each metabolism part of pathogens, thereby greatly slowing the development of drug resistance and reducing or even eliminating respective harmful side effects. Wherein epigallocatechin gallate (EGCG) is a flavanol compound in white or white powder, and is an important component of green tea polyphenols. EGCG not only has the effects of resisting oxidation, tumors, viruses, angiogenesis, thrombosis and the like, but also has strong bacteriostatic activity and has certain killing effect on most microorganisms including escherichia coli in nature. Curcumin (CUR) is a yellow powdery, bitter-tasting, hydrophobic polyphenol widely found in the rhizomes of various plants such as turmeric, curcuma aromatica, curcuma zedoary, calamus, etc. Modern pharmacological research shows that curcumin has pharmacological activities of antioxidation, antibiosis, anti-inflammation and the like.
Secondly, considering the technical scheme as a whole or from the perspective of products, the technical effect and advantages of the technical scheme to be protected by the invention are specifically described as follows:
the preparation method of the antibacterial drug based on the palladium nano material is simple and easy to operate, the cost is low, and the prepared antibacterial drug has a good antibacterial effect on candida albicans, escherichia coli and staphylococcus aureus.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for preparing an antibacterial drug based on a palladium nano-material according to an embodiment of the present invention;
FIG. 2 is a flow chart of a method for preparing a palladium nanomaterial provided by an embodiment of the invention;
FIG. 3 is a flow chart of the preparation method of fructus forsythiae extract and fructus Gardeniae extract according to the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
Aiming at the problems in the prior art, the invention provides an application of a palladium nano material in preparation of an antibacterial drug, and the invention is described in detail with reference to the accompanying drawings.
1. Illustrative embodiments are explained. This section is an illustrative example developed to explain the claims in order to enable those skilled in the art to fully understand how to implement the present invention.
The antibacterial drug based on the palladium nano material provided by the embodiment of the invention is composed of 30-50 parts of the palladium nano material, 20-30 parts of forsythia suspense extract, 15-20 parts of gardenia extract, 10-15 parts of epigallocatechin gallate, 8-12 parts of curcumin, 6-8 parts of a priomycin antibacterial compound and the balance of a drug additive in parts by mass; wherein the pharmaceutical additive is selected from one or more of magnesium carbonate, magnesium stearate, starch, talc, cyclodextrin, filler, disintegrant, lubricant or emulsifier.
The invention utilizes the mutual synergistic effect of the antibiotic composition of the pleiomycin, the palladium nano material, the epigallocatechin gallate, the curcumin, the forsythia extract and the gardenia extract to attack each metabolism part of pathogens, thereby greatly slowing the development of drug resistance and reducing or even eliminating respective harmful side effects. Wherein epigallocatechin gallate (EGCG) is a flavanol compound in white or white powder, and is an important component of green tea polyphenols. EGCG not only has the effects of resisting oxidation, tumors, viruses, angiogenesis, thrombosis and the like, but also has strong bacteriostatic activity and has certain killing effect on most microorganisms including escherichia coli in nature. Curcumin (CUR) is a yellow powdery, bitter-tasting, hydrophobic polyphenol widely found in the rhizomes of various plants such as turmeric, curcuma aromatica, curcuma zedoary and calamus. Modern pharmacological research shows that curcumin has pharmacological activities of antioxidation, antibiosis, anti-inflammation and the like.
As shown in fig. 1, a preparation method of an antibacterial drug based on a palladium nanomaterial provided by the embodiment of the present invention includes the following steps:
s101, preparing a palladium nano material: uniformly stirring and mixing a polyvinylpyrrolidone solution, an ascorbic acid solution and a citric acid solution according to a certain volume ratio; adding a sodium chloropalladate solution to react for a period of time under a certain condition, and then adding titanium dioxide and 3, 4-difluorothiophenol to obtain a palladium nano material;
s102, preparing epigallocatechin gallate: extracting green tea with alcohol to obtain crude green tea extract; acetylating polyphenol substance hydroxyl in green tea extractive crude liquid, separating, hydrolyzing in protonic solvent under acidic condition, and drying to obtain epigallocatechin gallate;
s103, preparing a fructus forsythiae extract and a fructus gardeniae extract: respectively weighing fructus forsythiae and fructus Gardeniae, mixing, extracting with ethanol under reflux, concentrating the extractive solution under reduced pressure, and dispersing in water to obtain a sample solution; adsorbing the sample solution with low-polarity or non-polar macroporous adsorbent resin, removing impurities, and drying under reduced pressure to obtain extract;
s104, preparing the antibacterial medicament: dissolving the medicine preparation raw materials, polylactic acid and polycaprolactone in an organic solvent to obtain a high polymer electrospinning solution; and (3) carrying out electrostatic spinning on the high polymer electrospinning solution under a certain condition, volatilizing the organic solvent, and drying in the dark to prepare the antibacterial drug based on the palladium nano material.
The preparation method of the antibacterial drug based on the palladium nano material is simple and easy to operate, the cost is low, and the prepared antibacterial drug has a good antibacterial effect on candida albicans, escherichia coli and staphylococcus aureus.
As shown in fig. 2, the preparation method of the palladium nanomaterial of step S101 provided by the embodiment of the present invention includes:
s201, preparing a polyvinylpyrrolidone solution, an ascorbic acid solution and a citric acid solution in sequence, placing the polyvinylpyrrolidone solution, the ascorbic acid solution and the citric acid solution in a stirring device in sequence according to a volume ratio of 1-3;
s202, adding a sodium chloropalladate solution into the mixed solution obtained in the S201, placing the mixture into a reaction device, uniformly stirring and mixing the mixture, and reacting for 4 to 6 hours at a temperature of between 90 and 110 ℃ to obtain a palladium nano material solution;
s203, adding titanium dioxide serving as a carrier into the palladium nano material solution obtained in the S202, adding 3, 4-difluorothiophenol, and stirring to obtain the palladium nano material with the size of 10-15 nm.
In the antibacterial drug based on the palladium nano material, the palladium nano material plays the roles of oxidase and peroxidase to achieve an antibacterial effect, but not release ions; through unique surface modification, the structure and the surface interface performance of the palladium nano material are changed, and a brand-new palladium nano material modified by mercaptan is obtained, so that the palladium nano material has a stable and lasting antibacterial effect.
The preparation method of epigallocatechin gallate in step S102 provided by the embodiment of the present invention includes:
(1) Placing a green tea medicinal material into an extraction device, heating and refluxing the green tea medicinal material by using 60% ethanol with the weight being 10-15 times that of the green tea medicinal material for 2-3 times, wherein each time lasts for 2-4 hours, and combining the respectively obtained extracting solutions to obtain a green tea extracting crude solution;
(2) Acetylating polyphenol substance hydroxyl in green tea extract crude liquid with acetylating agent, and separating with silica gel column chromatography to obtain acetylated epigallocatechin gallate;
(3) Hydrolyzing the acetylated epigallocatechin gallate in a protic solvent under acidic conditions to obtain epigallocatechin gallate, and drying to obtain epigallocatechin gallate.
As shown in fig. 3, the preparation method of the forsythia suspense extract and gardenia jasminoides ellis extract in step S103 according to the embodiment of the present invention includes:
s301, weighing fructus forsythiae and fructus gardeniae respectively according to the mass parts, mixing and placing the fructus forsythiae and the fructus gardeniae in an extraction device, and extracting the mixed material for 2-4 times by refluxing with 30-80% ethanol in an amount which is 8-12 times the mass parts, wherein the extraction time is 1-3 hours each time;
s302, cooling and centrifuging the extracting solution obtained in the step S301, filtering until filtrate is clear, combining the filtrate, concentrating under reduced pressure to obtain clear paste with the relative density of 1.25-1.45 g/mL, and adding sterilized deionized water for dispersing to obtain a sample solution;
and S303, adsorbing the sample solution obtained in the step S302 by using weak-polarity or non-polarity macroporous adsorption resin, eluting the resin by using water to remove impurities, collecting ethanol eluate, and drying under reduced pressure to obtain the extract.
The invention takes the relative terpenoid components and phenolic components in forsythia and gardenia as the basic components of the drug substance and adopts macroporous absorption resin to separate and enrich to obtain the drug substance with the antibacterial, synergistic and antibacterial effects; the fructus forsythiae extract and fructus Gardeniae extract have inhibitory effect on various pathogenic microorganisms, such as antibacterial, antifungal, antiviral, and leptospira killing effects.
The preparation method of the antibacterial agent in step S104 provided by the embodiment of the present invention includes:
(1) Dissolving a palladium nano material, a fructus forsythiae extract, a fructus gardeniae extract, epigallocatechin gallate, curcumin, a priomycin antibacterial compound, polylactic acid and polycaprolactone into an organic solvent to obtain a high polymer electrospinning solution, wherein the organic solvent is a mixed solution of ethanol and acetone in a volume ratio of 2;
(2) Under the conditions of 25-35 ℃ and 45-65% relative humidity, applying 10-25 kV voltage to the high polymer electrospinning solution, and carrying out electrostatic spinning at the advancing speed of 0.3-0.6 mL/min;
(3) Volatilizing the organic solvent from the electrostatic spinning reaction product obtained in the step (2), and naturally drying under a dark condition to prepare the antibacterial drug based on the palladium nano material.
The antibacterial drug loaded with the palladium nano material, the epigallocatechin gallate, the curcumin, the forsythia suspensa extract, the gardenia extract and the pleconycin antibacterial compound is prepared from the antibacterial drug and the biodegradable high polymer material by an electrospinning technology, has good biocompatibility and degradability, and can continuously and slowly release the internal drug, thereby achieving the purpose of effective antibiosis.
The preparation system of the antibacterial drug based on the palladium nano material provided by the embodiment of the invention comprises:
the palladium nano material preparation module is connected with the central control module and is used for sequentially placing polyvinylpyrrolidone, ascorbic acid and citric acid in the reaction device to prepare the palladium nano material;
the green tea crude extract preparation module is connected with the central control module and is used for placing a green tea medicinal material in an extraction device, heating, refluxing and extracting the green tea medicinal material by using ethanol for 2-3 times, and combining the extracting solution to obtain a green tea crude extract;
an epigallocatechin gallate preparation module connected with the central control module and used for acetylating hydroxyl in the green tea extraction crude liquid, separating and hydrolyzing, and drying to obtain epigallocatechin gallate;
the extract preparation module is connected with the central control module and is used for mixing fructus forsythiae and fructus gardeniae, putting the mixture into an extraction device, performing reflux extraction by using ethanol, and performing adsorption and impurity removal by using macroporous adsorption resin to obtain an extract;
the central control module is connected with each module of the preparation system and is used for coordinating and controlling the normal operation of each module of the preparation system of the antibacterial drug based on the palladium nano material through the central processing unit;
and the antibacterial drug preparation module is connected with the central control module and is used for dissolving the drug preparation raw materials, the polylactic acid and the polycaprolactone into an organic solvent, carrying out electrostatic spinning under a certain condition, volatilizing the organic solvent, and naturally drying under a dark condition to prepare the antibacterial drug based on the palladium nano material.
2. Application examples. In order to prove the creativity and the technical value of the technical scheme of the invention, the part is an application example of the technical scheme of the claims to a specific product or related technology.
An application embodiment of the present invention provides a computer program product stored on a computer readable medium, which includes a computer readable program for providing a user input interface to apply the steps of the method for preparing an antibacterial drug based on palladium nanomaterial when the computer program product is executed on an electronic device.
An application embodiment of the present invention provides a computer-readable storage medium storing instructions, which when executed on a computer, cause the computer to apply the steps of the method for preparing an antibacterial agent based on a palladium nanomaterial.
The application embodiment of the invention provides an information data processing terminal, which is used for realizing the preparation system of the antibacterial drug based on the palladium nano material.
It should be noted that embodiments of the present invention can be realized in hardware, software, or a combination of software and hardware. The hardware portions may be implemented using dedicated logic; the software portions may be stored in a memory and executed by a suitable instruction execution system, such as a microprocessor or specially designed hardware. Those skilled in the art will appreciate that the apparatus and methods described above may be implemented using computer executable instructions and/or embodied in processor control code, such code being provided on a carrier medium such as a disk, CD-or DVD-ROM, programmable memory such as read only memory (firmware), or a data carrier such as an optical or electronic signal carrier, for example. The apparatus and its modules of the present invention may be implemented by hardware circuits such as very large scale integrated circuits or gate arrays, semiconductors such as logic chips, transistors, or programmable hardware devices such as field programmable gate arrays, programmable logic devices, etc., or by software executed by various types of processors, or by a combination of hardware circuits and software, e.g., firmware.
The above description is only for the purpose of illustrating the embodiments of the present invention, and the scope of the present invention should not be limited thereto, and any modifications, equivalents and improvements made by those skilled in the art within the technical scope of the present invention as disclosed in the present invention should be covered by the scope of the present invention.
Claims (10)
1. A preparation method of an antibacterial drug based on a palladium nano material is characterized by comprising the following steps:
step one, preparing a palladium nano material: uniformly stirring and mixing a polyvinylpyrrolidone solution, an ascorbic acid solution and a citric acid solution according to a certain volume ratio; adding a sodium chloropalladate solution to react for a period of time under a certain condition, and then adding titanium dioxide and 3, 4-difluorothiophenol to obtain a palladium nano material;
step two, preparing epigallocatechin gallate: extracting green tea with alcohol to obtain crude green tea extract; acetylating polyphenol substance hydroxyl in green tea extractive crude liquid, separating, hydrolyzing in protonic solvent under acidic condition, and drying to obtain epigallocatechin gallate;
step three, preparing the forsythia suspense extract and the gardenia jasminoides extract: respectively weighing fructus forsythiae and fructus Gardeniae, mixing, reflux-extracting with ethanol, concentrating the extractive solution under reduced pressure, and dispersing in water to obtain a sample solution; adsorbing the sample solution with low-polarity or non-polar macroporous adsorbent resin, removing impurities, and drying under reduced pressure to obtain extract;
step four, preparing the antibacterial medicament: dissolving the raw materials for preparing the medicament, polylactic acid and polycaprolactone in an organic solvent to obtain high polymer electrospinning solution; and (3) carrying out electrostatic spinning on the high polymer electrospinning solution under a certain condition, volatilizing the organic solvent, and drying in the dark to prepare the antibacterial drug based on the palladium nano material.
2. The method for preparing an antibacterial drug based on palladium nanomaterial as claimed in claim 1, wherein the method for preparing the palladium nanomaterial in the first step comprises:
(1) Preparing a polyvinylpyrrolidone solution, an ascorbic acid solution and a citric acid solution in sequence, placing the polyvinylpyrrolidone solution, the ascorbic acid solution and the citric acid solution in a stirring device in sequence according to a volume ratio of 1-3;
(2) Adding a sodium chloropalladate solution into the mixed solution obtained in the step (1), placing the mixed solution into a reaction device, uniformly stirring and mixing, and reacting for 4-6 hours at the temperature of 90-110 ℃ to obtain a palladium nano material solution;
(3) And (3) adding titanium dioxide serving as a carrier into the palladium nano material solution obtained in the step (2), adding 3, 4-difluorothiophenol, and stirring to obtain the palladium nano material with the size of 10-15 nm.
3. The method for preparing an antibacterial drug based on palladium nanomaterial as claimed in claim 1, wherein the method for preparing epigallocatechin gallate in step two comprises:
(1) Placing the green tea medicinal material in an extraction device, heating and refluxing the green tea medicinal material for 2-3 times by using 60% ethanol with the weight being 10-15 times of that of the green tea medicinal material, extracting for 2-4 hours each time, and combining the respectively obtained extracting solutions to obtain a green tea extracting crude solution;
(2) Acetylating polyphenol substance hydroxyl in the green tea extract crude liquid by using an acetylating reagent, and separating by using silica gel column chromatography to obtain fully acetylated epigallocatechin gallate;
(3) Hydrolyzing the acetylated epigallocatechin gallate in a protic solvent under acidic conditions to obtain epigallocatechin gallate, and drying to obtain epigallocatechin gallate.
4. The method for preparing an antibacterial drug based on palladium nanomaterial as claimed in claim 1, wherein the method for preparing the forsythia suspense extract and the gardenia jasminoides ellis extract in step three comprises:
(1) Weighing fructus forsythiae and fructus gardeniae according to the mass parts respectively, mixing, placing the mixture into an extraction device, and extracting the mixture for 2 to 4 times by refluxing 30 to 80 percent ethanol in an amount which is 8 to 12 times of the mass parts, wherein each time of extraction is 1 to 3 hours;
(2) Cooling and centrifuging the extracting solution obtained in the step (1), filtering until filtrate is clear, combining, concentrating under reduced pressure to obtain clear paste with the relative density of 1.25-1.45 g/mL, and adding sterilized deionized water for dispersion to obtain a sample solution;
(3) And (3) adsorbing the sample liquid obtained in the step (2) by using weak-polarity or non-polarity macroporous adsorption resin, eluting the resin volume with water to remove impurities, collecting ethanol eluate, and drying under reduced pressure to obtain the extract.
5. The method for preparing an antibacterial agent based on palladium nanomaterial as claimed in claim 1, wherein the method for preparing an antibacterial agent in step four comprises:
(1) Dissolving a palladium nano material, a fructus forsythiae extract, a fructus gardeniae extract, epigallocatechin gallate, curcumin, a priomycin antibacterial compound, polylactic acid and polycaprolactone into an organic solvent to obtain a high polymer electrospinning solution, wherein the organic solvent is a mixed solution of ethanol and acetone in a volume ratio of 2;
(2) Under the conditions of 25-35 ℃ and 45-65% of relative humidity, applying 10-25 kV voltage to the high polymer electrospinning solution, and carrying out electrostatic spinning at the advancing speed of 0.3-0.6 mL/min;
(3) Volatilizing an organic solvent from the electrostatic spinning reaction product obtained in the step (2), and naturally drying under a dark condition to prepare the antibacterial drug based on the palladium nano material.
6. The antibacterial drug based on the palladium nano-material is prepared by the preparation method of the antibacterial drug based on the palladium nano-material according to any one of claims 1 to 5, and is characterized in that the antibacterial drug based on the palladium nano-material comprises 30 to 50 parts by mass of the palladium nano-material, 20 to 30 parts by mass of forsythia suspensa extract, 15 to 20 parts by mass of gardenia jasminoides extract, 10 to 15 parts by mass of epigallocatechin gallate, 8 to 12 parts by mass of curcumin, 6 to 8 parts by mass of a pleiomycin antibacterial compound and the balance of drug additives;
wherein the pharmaceutical additive is selected from one or more of magnesium carbonate, magnesium stearate, starch, talc, cyclodextrin, filler, disintegrant, lubricant or emulsifier.
7. A system for preparing an antibacterial agent based on palladium nanomaterial according to the method for preparing an antibacterial agent based on palladium nanomaterial of any one of claims 1 to 5, wherein the system for preparing an antibacterial agent based on palladium nanomaterial comprises:
the palladium nano material preparation module is connected with the central control module and is used for sequentially placing polyvinylpyrrolidone, ascorbic acid and citric acid in the reaction device to prepare the palladium nano material;
the green tea crude extract preparation module is connected with the central control module and is used for placing a green tea medicinal material in an extraction device, heating, refluxing and extracting the green tea medicinal material by using ethanol for 2-3 times, and combining the extracting solution to obtain a green tea crude extract;
an epigallocatechin gallate preparation module connected with the central control module and used for acetylating hydroxyl in the green tea extraction crude liquid, separating and hydrolyzing, and drying to obtain epigallocatechin gallate;
the extract preparation module is connected with the central control module and is used for mixing fructus forsythiae and fructus gardeniae, putting the mixture into an extraction device, performing reflux extraction by using ethanol, and performing adsorption and impurity removal by using macroporous adsorption resin to obtain an extract;
the central control module is connected with each module of the preparation system and is used for coordinating and controlling the normal operation of each module of the preparation system of the antibacterial drug based on the palladium nano material through the central processing unit;
and the antibacterial drug preparation module is connected with the central control module and is used for dissolving the drug preparation raw materials, the polylactic acid and the polycaprolactone into an organic solvent, carrying out electrostatic spinning under a certain condition, volatilizing the organic solvent, and naturally drying under a light-shading condition to prepare the antibacterial drug based on the palladium nano material.
8. A computer program product stored on a computer readable medium, comprising a computer readable program for providing a user input interface for applying the steps of the method of preparing a palladium nanomaterial-based antimicrobial drug of any one of claims 1 to 5 when executed on an electronic device.
9. A computer-readable storage medium storing instructions which, when executed on a computer, cause the computer to apply the steps of the method for preparing an antibacterial agent based on a palladium nanomaterial according to any one of claims 1 to 5.
10. An information data processing terminal, characterized in that the information data processing terminal is used for implementing the system for preparing antibacterial drugs based on palladium nano-materials according to claim 7.
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