CN115575319A - Luminescence analysis device and sensitivity adjustment method for luminescence analysis device - Google Patents
Luminescence analysis device and sensitivity adjustment method for luminescence analysis device Download PDFInfo
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- CN115575319A CN115575319A CN202210686206.9A CN202210686206A CN115575319A CN 115575319 A CN115575319 A CN 115575319A CN 202210686206 A CN202210686206 A CN 202210686206A CN 115575319 A CN115575319 A CN 115575319A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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- G01N21/763—Bioluminescence
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- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/124—Sensitivity
- G01N2201/1244—Ambient light detector, e.g. for invalidating
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
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Abstract
The invention provides a luminescence analysis device and a sensitivity adjustment method thereof, which can accurately judge the sensitivity change of a photodetector without cost and can realize the miniaturization of the device. The luminescence analysis apparatus obtains a concentration of a component to be measured in a sample solution based on a luminescence amount from a measurement liquid (4) containing the sample solution and a luminescent reagent that emits light by reacting with the component to be measured in the sample solution, and a calibration curve, and is characterized by comprising: a photodetector (20) that detects light from the measurement liquid (4); and an arithmetic unit (30) to which the light emission amount detected by the light detector (20) is input, wherein the arithmetic unit (30) compares the light emission amount detected by the light detector (20) when a blank solution not containing the measurement target component is used as the sample solution in the measurement solution 4 with the light emission amount at which the concentration of the measurement target component in the calibration curve is zero, and confirms the sensitivity of the light detector (20).
Description
Technical Field
The present invention relates to a luminescence analysis apparatus and a sensitivity adjustment method for the luminescence analysis apparatus. More specifically, the present invention relates to a luminescence analysis apparatus suitable for luminescence analysis by a bioluminescence method and a chemiluminescence method, and a sensitivity adjustment method thereof.
Background
Since the bioluminescence method and the chemiluminescence method can quantify a trace amount of a substance in a sample solution, they are used in various fields such as medicine, biochemistry, clinical examination, agriculture, and food.
For example, patent document 1 discloses a bioluminescence method for detecting endotoxin with high sensitivity, which frees a luminescent substrate from a synthetic substrate by a reagent activated by endotoxin and causes the free luminescent substrate to emit light.
In many cases, reagents derived from living organisms are used as luminescent reagents used in luminescence analysis, particularly in luminescence analysis by a bioluminescence method, and the sensitivity of each lot is different from each other. Therefore, a calibration curve showing the relationship between the analyte and the amount of luminescence is obtained for each batch of the luminescent reagent.
Documents of the prior art
Patent document
Patent document 1: international publication No. 2009/063840
Disclosure of Invention
Problems to be solved by the invention
However, the sensitivity of the photodetector used in the luminescence analysis device changes with the passage of time, and the luminescence amount may not be accurately measured.
Therefore, it is conceivable to provide a light source as a reference in the apparatus, detect the light emission amount from the light source by a light detector, check the change in the sensitivity of the light detector, and correct the measured light emission amount.
However, in this case, since the light source as a reference must be provided in the apparatus, not only the cost is increased, but also a space for providing the light source needs to be prepared, and thus it is difficult to downsize the apparatus. Further, when the light emission amount of the light source itself as a reference changes with the passage of time, the sensitivity of the photodetector cannot be accurately determined.
The present invention has been made in view of the above circumstances, and an object thereof is to provide a luminescence analysis device and a sensitivity adjustment method thereof, which can accurately determine a change in sensitivity of a photodetector without a cost and can achieve a reduction in size of the device.
Technical scheme for solving problems
In order to achieve the above object, the present invention adopts the following configuration.
A first aspect of the present invention provides a luminescence analysis apparatus for determining a concentration of a measurement target component in a sample solution based on a luminescence amount of a measurement solution from a measurement solution containing the sample solution and a luminescent reagent that emits light by reacting with the measurement target component in the sample solution, and a calibration curve of the luminescent reagent, the luminescence analysis apparatus comprising:
a photodetector that detects light from the measurement liquid; and
an arithmetic device to which the light emission amount detected by the photodetector is input,
the arithmetic device compares the light emission amount detected by the photodetector when a blank solution not containing the component to be measured is used as the sample solution in the measurement liquid with the light emission amount of the calibration curve when the concentration of the component to be measured is zero, and confirms the sensitivity of the photodetector.
The invention according to claim 2 is the luminescence analysis apparatus according to claim 1, wherein the calibration curve is a calibration curve determined for each lot of the luminescent reagent.
In accordance with a third aspect of the present invention, there is provided a luminescence analysis apparatus for determining a concentration of a measurement target component in a sample solution based on a luminescence amount of a measurement solution containing the sample solution, a luminescent reagent that emits light by reacting with the measurement target component, and a luminescent substance, and a calibration curve of the luminescent reagent and the luminescent substance, the luminescence analysis apparatus comprising:
a photodetector that detects light from the measurement liquid; and
an arithmetic device to which the light emission amount detected by the photodetector is input,
the arithmetic device compares the amount of luminescence detected by the photodetector when a blank solution not containing the component to be measured is used as the sample solution in the measurement liquid with the amount of luminescence of the calibration curve when the concentration of the component to be measured is zero, and confirms the change in sensitivity of the photodetector.
The invention according to claim 4 is the luminescence analysis apparatus according to claim 3, wherein the calibration curve is a calibration curve determined for each lot of the luminescent reagent and the luminescent substance.
The invention according to claim 5 is the luminescence analysis apparatus according to any one of claims 1 to 4, wherein the photodetector is a photomultiplier tube.
A 6 th aspect of the present invention is the sensitivity adjustment method for a luminescence analysis apparatus according to the 5 th aspect, wherein when the computing unit determines that the sensitivity of the photodetector has changed beyond a predetermined allowable range, the computing unit changes the voltage applied to the photodetector to adjust the sensitivity of the photodetector.
The invention according to claim 7 is the method for adjusting sensitivity of a luminescence analysis apparatus according to claim 6, wherein a reagent that emits light by a bioluminescence phenomenon is used as the luminescent reagent.
Effects of the invention
According to the luminescence analysis apparatus and the sensitivity adjustment method thereof of the present invention, not only can the sensitivity change of the photodetector be accurately judged without cost, but also the apparatus can be miniaturized.
Drawings
Fig. 1 is a schematic configuration diagram of a luminescence analysis apparatus according to an embodiment of the present invention.
Detailed Description
[ embodiment 1 ]
Fig. 1 shows a luminescence analysis apparatus according to an embodiment of the present invention. The luminescence analysis apparatus of the present embodiment includes: a measuring chamber 10 in which the measuring container 1 can be stored; a photodetector 20 that detects light from the inside of the measurement container 1 accommodated in the measurement chamber 10; and an arithmetic device 30 to which the light emission amount detected by the light detector 20 is input.
The measurement chamber 10 is composed of a bottomed cylindrical measurement chamber main body 11 having an opening 11a at an upper end thereof, and a door 12 covering the opening 11a in an openable and closable manner.
The measurement chamber body 11 is shielded from light except for a portion of the light-transmitting detection window 11b provided in the side surface. Further, the door 12 is also shielded from light.
When the door 12 is in an open state (the state of the door 12 indicated by a broken line in fig. 1), the measurement container 1 can be taken in and out of the measurement chamber main body 11 through the opening 11 a. When the door 12 is in the closed state (the state of the door 12 indicated by the solid line in fig. 1), the measurement chamber 10 can block the entrance of external light into the measurement chamber 10.
As the photodetector 20, a photomultiplier tube, a photodiode, a phototransistor, an avalanche photodiode, or the like can be suitably used.
In the present embodiment, the light detector 20 detects light only when the door 12 is in the closed state. For example, when the photodetector 20 is a photomultiplier tube, the photodetector 20 is energized only when the door 12 is in the closed state.
The method of detecting light by the light detector 20 only when the door 12 is in the closed state is not particularly limited, and may be, for example: a method of closing a circuit for applying a voltage to the photodetector 20 by bringing the distal end of the door 12 into contact with the periphery of the opening 11a of the measurement chamber main body 11 with the door 12 itself as an open/close switch. Further, a touch sensor or the like that senses that the distal end of the door 12 touches the periphery of the opening 11a of the measurement chamber main body 11 may be used.
The measurement container 1 accommodated in the measurement chamber 10 is composed of a bottomed cylindrical container body 2 having an open upper end, and a lid 3 that closes the upper end of the container body 2 in a liquid-tight manner.
The container body 2 is made of a transparent material such as glass to ensure sufficient light transmission at least in the wavelength range of light to be detected by the photodetector 20.
In embodiment 1, the measurement liquid 4 contained in the measurement container 1 includes a sample liquid and a luminescent reagent that emits light by reacting with a component to be measured in the sample liquid.
In the present invention, the light-emitting reagent means a reagent or the whole reagent set required for generating light emission according to the concentration of a component to be measured, and a reagent set composed of a plurality of reagents generally corresponds to the light-emitting reagent.
For example, when the component to be measured is a microbial impurity such as endotoxin or β -glucan, and when the analysis is performed by utilizing the bioluminescence phenomenon, a reagent activated by the microbial impurity, a luminescent synthesis substrate which releases a luminescent substrate by the activating reagent activated by the microbial impurity, a luminescent enzyme which emits light from the released luminescent substrate, and a luminescent reagent containing another compound required for a luminescent reaction are used.
Next, the luminescent reagent in the case where the component to be measured is endotoxin will be described in detail.
The reagent activated by endotoxin is preferably a reagent containing factor C activated by binding to endotoxin, and more preferably a reagent containing factor B activated by active factor C and proclotting enzyme activated by active factor B to produce clotting enzyme in addition to factor C. As the reagent containing factor C, factor B and proclotting enzyme, a limulus blood cell extract (lysis reagent) can be preferably used.
As the luminescent synthesis substrate, a luminescent synthesis substrate in which a luminescent substrate is bound to a peptide can be used. As the luminescent synthesis substrate when the component to be measured is endotoxin, a substance having the following structure can be used: a structure in which the binding between the luminescent substance and the peptide is cleaved by the action of at least one of the active factor C, the active factor B, and the clotting enzyme (protease activity).
As the light-emitting substrate, aminofluorescein can be preferably used. The peptide to be bound to the light-emitting substrate may be one composed of the following amino acid sequence: an amino acid sequence in which the amide bond to the aminofluorescein at the C-terminal end of the peptide is cleaved by the protease activity of at least one of active factor C, active factor B and coagulase.
The luminescent enzyme is an enzyme that generates light by functioning as a catalyst for bioluminescence of a luminescent substrate that is free from a luminescent synthesis substrate. The light-emitting enzyme when the light-emitting substrate is aminoluciferin is luciferase, and other compounds required for the light-emitting reaction are ATP and divalent metal ions.
When the sample liquid contains a salt, the luminescent reagent may further contain NaCl in order to eliminate an error caused by the salt concentration.
Preferably, the luminescent reagent is contained in the measurement vessel 1 in advance before the sample liquid is injected into the measurement vessel 1. For example, the luminescent reagent may be attached to the bottom of the container main body 2 in a freeze-dried state in advance.
The luminescence reagent is stored in the measurement container 1 in advance, and the sample liquid is simply injected into the measurement container 1, whereby the luminescence reagent can be used for analysis by the luminescence analysis apparatus of the present embodiment.
When an unknown sample whose concentration of a component to be measured is unknown is used as a sample liquid in the measurement liquid 4, the arithmetic unit 30 determines the concentration of the component to be measured in the sample liquid based on the light emission amount detected by the light detector 20.
Specifically, the concentration of the component to be measured in the sample liquid is determined by comparing the amount of luminescence detected by the photodetector 20 when the measurement container 1 containing the measurement liquid 4 is stored in the measurement chamber 10 with a calibration curve stored in advance.
The arithmetic device 30 stores a calibration curve showing a relationship between the light emission amount and the concentration of the measurement target component in advance. The calibration curve is determined for each lot of luminescent reagent, and when a new lot of luminescent reagent is used, the calibration curve is updated by inputting information of the calibration curve provided by a manufacturer or the like.
Preferably, the determined concentration of the component to be measured can be displayed on a display device built in the luminescence analysis device of the present embodiment or on a separate display device, and can be output to a built-in or separate printer or an external computer.
Preferably, the arithmetic device 30 performs an operation of determining the concentration of the measurement target component in the sample liquid when determining that the measurement container 1 is already stored in the measurement chamber 10.
Accordingly, the arithmetic device 30 can automatically start the operation of determining the concentration of the component to be measured in the sample liquid only by storing the measurement container 1 in the measurement chamber 10.
For example, when the door 12 is opened and then closed, the arithmetic device 30 can determine whether or not the measurement container 1 is stored in the measurement chamber 10 based on whether or not the photodetector 20 can detect the background light emission of the luminescent reagent itself.
Specifically, whether the background light emission is detectable is determined based on whether or not the light emission amount detected by the light detector 20 is equal to or greater than a predetermined threshold.
The threshold is a luminescence amount that can be clearly determined that background luminescence of the luminescent reagent itself has occurred.
The measurement container 1 itself may generate extremely minute natural light emission, but the amount of light emission of such natural light emission or the amount of light emission close to the amount of light emission should not be used as a threshold. This is because, if an extremely small amount of luminescence, such as natural luminescence emitted from the measurement container 1 itself, is used as a threshold, the photodetector 20 having extremely high sensitivity has to be prepared, and it is difficult to accurately determine whether or not background luminescence is present.
The amount of luminescence of the background luminescence of the luminescent reagent itself can be confirmed by measuring the amount of luminescence of the measurement liquid 4 containing the blank solution and the luminescent reagent using a sample liquid containing no component to be measured as the blank solution.
The threshold value is preferably 10% to 100%, more preferably 30% to 70%, for example, about 50% of the amount of background luminescence of the luminescent reagent itself.
When the sensitivity of the photodetector 20 is confirmed, a blank solution containing no component to be measured is used as the sample solution in the measurement solution 4. Then, the operator stores the measurement container 1 containing the measurement solution 4 including the blank solution in the measurement chamber 10 and performs an input operation requiring confirmation of the sensitivity.
Then, the arithmetic device 30 compares the light emission amount detected by the photodetector 20 with the light emission amount of the calibration curve stored in advance when the concentration of the component to be measured is zero, and confirms the change in the sensitivity of the photodetector 20.
Here, the amount of luminescence when the concentration of the component to be measured of the calibration curve stored in advance is zero (hereinafter, may be referred to as "zero reference luminescence") corresponds to the background luminescence of the luminescent reagent itself. The background luminescence of the luminescent reagent itself is generated, for example, by luminescence of free luminescent substrates contained in the luminescent synthesis matrix due to the luminescent enzyme.
The arithmetic device 30 compares the light emission amount detected by the light detector 20 with a zero reference light emission amount. For example, the comparison is performed by obtaining a ratio of the light emission amount detected by the photodetector 20 to the zero reference light emission amount or a difference therebetween.
When the ratio of the light emission amount detected by the photodetector 20 to the zero reference light emission amount is obtained, the arithmetic device 30 determines that the sensitivity of the photodetector 20 has changed beyond a predetermined allowable range when the obtained ratio is smaller than or exceeds a ratio of a predetermined range.
When the difference between the two is obtained, if the obtained difference exceeds a difference within a predetermined range, the arithmetic device 30 determines that the sensitivity of the photodetector 20 has exceeded a predetermined allowable range and has changed.
The predetermined range may be set as appropriate in accordance with the required measurement accuracy or the like, and may be stored in the arithmetic device 30.
When the computing device 30 confirms that the sensitivity of the photodetector 20 has changed beyond a predetermined allowable range, it may cause the operator to take measures such as adjusting the sensitivity of the photodetector 20 or replacing the photodetector 20 by outputting an alarm or the like.
If the photodetector 20 is a photomultiplier tube, the sensitivity of the photodetector 20 can be adjusted by changing the voltage applied to the photodetector 20.
[ 2 nd embodiment ]
The device configuration itself of the luminescence analysis device according to embodiment 2 is the same as that of the first embodiment, and includes, as shown in fig. 1: a measuring chamber 10 in which the measuring container 1 can be stored; a photodetector 20 that detects light from the inside of the measurement container 1 accommodated in the measurement chamber 10; and an arithmetic device 30 to which the light emission amount detected by the light detector 20 is input.
The luminescence analysis apparatus according to embodiment 2 differs in the measurement liquid 4 contained in the measurement container 1. The operation of the arithmetic device 30 differs depending on the measurement liquid 4. The other items are the same as those of the emission analysis device of embodiment 1, and therefore, the description thereof is omitted.
In embodiment 2, the measurement liquid 4 contained in the measurement container 1 contains a sample liquid, and a luminescent reagent and a luminescent substance that react with a component to be measured in the sample liquid to emit light.
As described in embodiment 1, the luminescent reagent means a reagent or the whole reagent group necessary for generating luminescence according to the concentration of a component to be measured, and a reagent group composed of a plurality of reagents generally corresponds to the luminescent reagent.
In the present invention, the light-emitting substance is a substance that emits light by a light-emitting reagent in the absence of a component to be measured, or a substance that emits light by itself, and may be a reagent group composed of a plurality of reagents.
The wavelength range of light emitted from the light-emitting substance is preferably equal to, more preferably equal to, the wavelength range of light emitted by the component to be measured acting on the light-emitting reagent. Accordingly, when a blank solution is used as the sample liquid, the amount of luminescence detected by the photodetector 20 can be increased.
In the case where the component to be measured is not present, the substance that emits light by the luminescent reagent may be a luminescent substrate that emits light by a luminescent enzyme contained in the luminescent reagent, for example, luciferin or the like in the case where the luminescent enzyme is firefly luciferase.
As the substance which itself emits light, there may be mentioned a combination of another luminescent enzyme other than the luminescent enzyme contained in the luminescent reagent and a luminescent substrate which emits light due to the other luminescent enzyme, for example, a combination of click beetle luciferase and luciferin, a combination of Renilla luciferase and coelenterazine, or the like.
When the light-emitting substance is a light-emitting substrate that emits light due to a luminescent enzyme contained in the light-emitting reagent, a light-emitting substrate that emits light in a wavelength range equal to that of a light-emitting substrate that is released from the light-emitting synthesis substrate contained in the light-emitting reagent is preferable, and a light-emitting substrate that is the same as a light-emitting substrate that is released from the light-emitting synthesis substrate contained in the light-emitting reagent is particularly preferable.
For example, when the luminescent reagent contains a luminescent synthesis substrate in which aminoluciferin is released and the luminescent enzyme contains luciferase, aminoluciferin is preferably used as the luminescent substance.
When the light-emitting substance is a combination of another luminescent enzyme different from the luminescent enzyme contained in the light-emitting reagent and a light-emitting substrate that emits light due to the other luminescent enzyme, it is preferable that light in a wavelength range equivalent to that of a light-emitting substrate free from the light-emitting synthesis substrate contained in the light-emitting reagent is emitted by the combination.
For example, when the luminescent reagent contains a luminescent synthesis substrate in which aminoluciferin is released and luciferase is used as the luminescent enzyme, a combination of click beetle luciferase and luciferin is preferably used.
Preferably, the luminescent substance and the luminescent reagent are contained in the measurement vessel 1 in advance before the sample liquid is injected into the measurement vessel 1. For example, the luminescent reagent and the luminescent substance may be attached to the bottom of the container main body 2 in a freeze-dried state in advance.
The luminescence reagent and the luminescent substance may be contained in the measurement cell 1 in advance, and the sample liquid may be simply poured into the measurement cell 1, and the luminescence reagent and the luminescent substance may be used for analysis by the luminescence analysis apparatus of the present embodiment.
Similarly, in the present embodiment, when an unknown sample whose concentration of a component to be measured is unknown is used as a sample liquid in the measurement liquid 4, the arithmetic unit 30 determines the concentration of the component to be measured in the sample liquid based on the light emission amount detected by the light detector 20.
Specifically, the concentration of the component to be measured in the sample liquid is determined by comparing the amount of luminescence detected by the photodetector 20 when the measurement container 1 containing the measurement liquid 4 is stored in the measurement chamber 10 with a calibration curve stored in advance.
The arithmetic device 30 stores a calibration curve showing a relationship between the light emission amount and the concentration of the measurement target component in advance. The calibration curve is determined for each combination of the luminescent reagent and the luminescent substance, and when a new lot of the luminescent reagent and the luminescent substance is used, the calibration curve is updated by inputting information of the calibration curve provided by a manufacturer or the like.
Preferably, the determined concentration of the component to be measured can be displayed on a display device built in the luminescence analysis device of the present embodiment or on a separate display device, and can be output to a built-in or separate printer or an external computer.
Similarly, in the present embodiment, it is preferable that the arithmetic unit 30 performs an operation of determining the concentration of the measurement target component in the sample liquid when determining that the measurement container 1 is already stored in the measurement chamber 10.
Accordingly, the arithmetic device 30 can automatically start the operation of determining the concentration of the measurement target component in the sample liquid by simply storing the measurement container 1 in the measurement chamber 10.
For example, when the door 12 is opened and then closed, the arithmetic device 30 can determine whether or not the measurement container 1 is already stored in the measurement chamber 10 based on whether or not the photodetector 20 detects the light emission combined with the background light emission of the luminescent reagent itself and the light emission of the luminescent substance.
The method of determining the open/close state of the door 12 by the arithmetic device 30 is the same as that of embodiment 1.
The amount of luminescence of the luminescence obtained by combining the background luminescence of the luminescent reagent itself and the luminescence of the luminescent substance is larger than that obtained by only the background luminescence of the luminescent reagent itself. Therefore, it is possible to more easily determine whether or not the measurement container 1 has been stored in the measurement chamber 10.
Specifically, it is determined whether or not the light emission in which the background light emission and the light-emitting substance are combined can be detected, based on whether or not the light emission amount detected by the photodetector 20 is equal to or greater than a predetermined threshold value.
The threshold is a luminescence amount at which it can be clearly determined that luminescence occurs by the background luminescence of the luminescent reagent itself and the luminescence of the luminescent substance.
In the present embodiment, similarly, the amount of natural light emitted from the measurement cell 1 or the amount of light close to the amount of natural light emission should not be used as a threshold.
The luminescence amount of the luminescence reagent itself combined with the background luminescence and the luminescence of the luminescent substance can be confirmed by measuring the luminescence amount of the measurement liquid 4 containing the blank solution, the luminescent reagent and the luminescent substance using a sample liquid containing no component to be measured as the blank solution.
The threshold value is preferably 10% to 100%, more preferably 30% to 70%, for example, about 50% of the amount of luminescence of the luminescence obtained by combining the background luminescence of the luminescent reagent itself and the luminescence of the luminescent substance.
When the sensitivity of the photodetector 20 is confirmed, a blank solution containing no component to be measured is used as the sample solution in the measurement solution 4. Then, the operator stores the measurement container 1 containing the measurement solution 4 including the blank solution in the measurement chamber 10 and performs an input operation requiring confirmation of the sensitivity.
Then, the arithmetic device 30 compares the light emission amount detected by the photodetector 20 with the light emission amount of the calibration curve stored in advance when the concentration of the component to be measured is zero, and confirms the change in the sensitivity of the photodetector 20.
Here, the luminescence amount when the concentration of the component to be measured of the calibration curve stored in advance is zero corresponds to luminescence in which the background luminescence of the luminescent reagent itself and the luminescence of the luminescent substance are combined.
The method of comparison and the method of using the comparison result are the same as those of embodiment 1.
According to the luminescence analysis device of each of the above embodiments, the change in sensitivity of the photodetector 20 can be accurately determined without providing a light source in the device. Therefore, the size and cost of the device can be reduced.
Description of the reference numerals
1. Measuring container
2. Container body
3. Cover
4. Assay solution
10. Measuring chamber
11. Measuring chamber body
11a opening part
11b detection window
12. Door with a door panel
20. Light detector
30. And an arithmetic device.
Claims (7)
1. A luminescence analysis apparatus for determining a concentration of a measurement target component in a sample solution based on a luminescence amount of a measurement solution from a measurement solution containing the sample solution and a luminescent reagent that emits light by reacting with the measurement target component in the sample solution, and a calibration curve of the luminescent reagent, the luminescence analysis apparatus comprising:
a photodetector that detects light from the measurement liquid; and
an arithmetic device to which the light emission amount detected by the photodetector is input,
the arithmetic unit compares the luminescence amount detected by the photodetector when a blank solution not containing the measurement target component is used as the sample solution in the measurement solution with the luminescence amount of the calibration curve when the concentration of the measurement target component is zero, and confirms the sensitivity of the photodetector.
2. The luminescence analysis apparatus according to claim 1,
the calibration curve is a calibration curve determined for each batch of the luminescent reagent.
3. A luminescence analysis apparatus for determining a concentration of a measurement target component in a sample solution based on a luminescence amount from a measurement solution containing the sample solution, a luminescent reagent that emits light by reacting with the measurement target component, and a luminescent substance, and a calibration curve of the luminescent reagent and the luminescent substance, the luminescence analysis apparatus comprising:
a photodetector that detects light from the measurement liquid; and
an arithmetic device to which the light emission amount detected by the photodetector is input,
the arithmetic device compares the amount of luminescence detected by the photodetector when a blank solution not containing the component to be measured is used as the sample solution in the measurement liquid with the amount of luminescence of the calibration curve when the concentration of the component to be measured is zero, and confirms the change in sensitivity of the photodetector.
4. A luminescence analysis apparatus according to claim 3,
the calibration curve is a calibration curve determined for each batch of the luminescent reagent and the luminescent substance.
5. The luminescence analysis apparatus according to any one of claims 1 to 4,
the light detector is a photomultiplier tube.
6. A method for adjusting the sensitivity of a luminescence analysis apparatus according to claim 5,
when the computing device determines that the sensitivity of the photodetector has changed beyond a predetermined allowable range, the computing device adjusts the sensitivity of the photodetector by changing the voltage applied to the photodetector.
7. The sensitivity adjustment method for a luminescence analysis apparatus according to claim 6,
as the light-emitting agent, an agent that emits light by a bioluminescence phenomenon is used.
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| JP2021102391A JP7417117B2 (en) | 2021-06-21 | 2021-06-21 | Emission spectrometer and sensitivity adjustment method for emission spectrometer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| SE428379B (en) * | 1978-05-31 | 1983-06-27 | Lkb Produkter Ab | DETERMINATION OF ATOL AND REAGENTS OF BIOLUMINISM |
| JPS6318900Y2 (en) * | 1978-09-29 | 1988-05-27 | ||
| JP2000046734A (en) * | 1998-07-28 | 2000-02-18 | Olympus Optical Co Ltd | Device for measuring small quantity of light |
| JP3793782B2 (en) * | 2000-07-24 | 2006-07-05 | アークレイ株式会社 | Measuring apparatus, reagent carrier and information recording medium used therefor, measuring data correction method, and program recording medium |
| JP2007330185A (en) * | 2006-06-16 | 2007-12-27 | Toyo B-Net Co Ltd | Method for detecting two or more luciferases in multispecimen sample |
| JP5669528B2 (en) * | 2010-11-17 | 2015-02-12 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP6546374B2 (en) * | 2014-07-21 | 2019-07-17 | 株式会社サカエ | Automatic analyzer |
| JP6434114B1 (en) * | 2017-11-30 | 2018-12-05 | シスメックス株式会社 | Measuring method and measuring device |
| JP6836522B2 (en) * | 2018-01-26 | 2021-03-03 | 株式会社日立ハイテク | Automatic analyzer and control method of automatic analyzer |
-
2021
- 2021-06-21 JP JP2021102391A patent/JP7417117B2/en active Active
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2022
- 2022-06-16 CN CN202210686206.9A patent/CN115575319A/en active Pending
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| JP2023001580A (en) | 2023-01-06 |
| JP7417117B2 (en) | 2024-01-18 |
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