CN115558151B - Aerogel taking mung bean protein and burdock nanocellulose synergistically stabilized foam as template - Google Patents
Aerogel taking mung bean protein and burdock nanocellulose synergistically stabilized foam as template Download PDFInfo
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- CN115558151B CN115558151B CN202211026457.0A CN202211026457A CN115558151B CN 115558151 B CN115558151 B CN 115558151B CN 202211026457 A CN202211026457 A CN 202211026457A CN 115558151 B CN115558151 B CN 115558151B
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- 235000008078 Arctium minus Nutrition 0.000 title claims abstract description 94
- 239000004964 aerogel Substances 0.000 title claims abstract description 41
- 229920001046 Nanocellulose Polymers 0.000 title claims abstract description 37
- 239000006260 foam Substances 0.000 title claims abstract description 29
- 235000019707 mung bean protein Nutrition 0.000 title claims abstract description 24
- 240000005528 Arctium lappa Species 0.000 title 1
- 241000208843 Arctium Species 0.000 claims abstract description 93
- 239000000835 fiber Substances 0.000 claims abstract description 37
- 239000000843 powder Substances 0.000 claims abstract description 24
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- 239000001509 sodium citrate Substances 0.000 claims abstract description 9
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 9
- 238000004108 freeze drying Methods 0.000 claims abstract description 8
- 108010059892 Cellulase Proteins 0.000 claims abstract description 7
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims abstract description 7
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- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 7
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- 102000013142 Amylases Human genes 0.000 claims abstract description 5
- 108010065511 Amylases Proteins 0.000 claims abstract description 5
- 235000019418 amylase Nutrition 0.000 claims abstract description 5
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- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 description 18
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- 235000012754 curcumin Nutrition 0.000 description 9
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- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 description 9
- 230000029087 digestion Effects 0.000 description 7
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- 102000004882 Lipase Human genes 0.000 description 4
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- 239000002285 corn oil Substances 0.000 description 4
- 235000005687 corn oil Nutrition 0.000 description 4
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- 238000002360 preparation method Methods 0.000 description 4
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- 239000002131 composite material Substances 0.000 description 2
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- 239000000126 substance Substances 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/28—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof by elimination of a liquid phase from a macromolecular composition or article, e.g. drying of coagulum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/38—Cellulose; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2205/00—Foams characterised by their properties
- C08J2205/02—Foams characterised by their properties the finished foam itself being a gel or a gel being temporarily formed when processing the foamable composition
- C08J2205/026—Aerogel, i.e. a supercritically dried gel
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2205/00—Foams characterised by their properties
- C08J2205/04—Foams characterised by their properties characterised by the foam pores
- C08J2205/042—Nanopores, i.e. the average diameter being smaller than 0,1 micrometer
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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- C08J2301/00—Characterised by the use of cellulose, modified cellulose or cellulose derivatives
- C08J2301/02—Cellulose; Modified cellulose
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2401/00—Characterised by the use of cellulose, modified cellulose or cellulose derivatives
- C08J2401/02—Cellulose; Modified cellulose
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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- C08J2489/00—Characterised by the use of proteins; Derivatives thereof
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Abstract
The invention relates to an aerogel taking mung bean protein and burdock nanocellulose synergistically stabilized foam as a template, which comprises the following steps: fresh burdock is selected as a raw material, dried after cleaning, superfine crushed and sieved, burdock fine powder and water are mixed and stirred uniformly, amylase, saccharifying enzyme and papain are respectively added, water bath boiling is carried out for enzyme deactivation, and then the burdock fiber is obtained through centrifugation. Washing and centrifuging the obtained burdock fiber for many times to remove trace impurities to obtain a fiber solution, freeze-drying the fiber solution to obtain burdock fiber powder, dispersing the burdock fiber powder in a sodium citrate buffer solution containing xylanase and cellulase, boiling the burdock fiber powder after stirring to inactivate the enzyme, centrifuging the burdock fiber after ultrasonic treatment, collecting precipitate, and washing the precipitate with absolute ethyl alcohol to obtain the burdock nanocellulose. Homogenizing burdock nanocellulose and mung bean protein to form foam. And (5) putting the foam into a refrigerator for freeze drying to obtain the aerogel. Compared with the prior aerogel, the burdock serving as the raw material has low cost and is easy to obtain, and the cost of the aerogel can be reduced.
Description
Technical Field
The invention relates to aerogel taking mung bean protein and burdock nanocellulose synergistically stabilized foam as a template, and belongs to the technical field of aerogel biology.
Background
Although the burdock is widely planted in China, the utilization rate of the burdock in China is not high, the development of deep products is lacking, and the waste of the existing burdock resources is caused to a great extent. The burdock root serving as the main edible part of burdock contains rich dietary fibers, and in root plants, the burdock has the advantages of complete biodegradability, easiness in modification, good biocompatibility and the like, and finer nano cellulose is prepared through finish machining.
Aerogel is a novel functional material, is a porous solid, has the surface spread over nanoscale pore channels, high porosity and large specific surface area, and has excellent porosity and specific surface area, so that the aerogel provides structural support for the fields of foods, medicines, bioscience, adsorption and the like. The three-dimensional pore structure of the aerogel can play a role in slow release in the fields of active substance loading and drug delivery, and has good application prospect.
However, the price of the aerogel is high because the aerogel is manufactured by manually using chemical substances, and the cost is high, so that more different raw materials are needed to solve the problem.
Disclosure of Invention
The invention aims to: aiming at the problems and the defects existing in the prior art, the invention aims to provide the aerogel taking the mung bean protein and burdock nanocellulose synergistically stabilized foam as a template, which is a white spongy porous solid and has good mechanical property and adsorptivity. The novel composite material is formed by mixing mung bean proteins, the biocompatibility of the burdock nanocellulose is improved, and meanwhile, the high-strength nano structure of the burdock nanocellulose is utilized to improve the strength of the burdock nanocellulose and mung bean protein aerogel composite material. The method can be used for exploring the application of aerogel materials taking foam as a template in aspects of structured grease, targeted delivery of functional factors and the like. Compared with the aerogel used at present, the burdock is used as the raw material, has low cost and is easy to obtain, and the cost of the aerogel can be reduced.
The technical scheme is as follows: in order to achieve the above purpose, the present invention adopts the following technical scheme:
an aerogel taking mung bean protein and burdock nanocellulose synergistically stabilized foam as a template is characterized in that: the method comprises the following steps:
step 1: pretreatment of raw materials: selecting fresh burdock as a raw material, cleaning, drying, ultrafine grinding and sieving to obtain burdock fine powder;
step 2: preparing burdock fibers: mixing the burdock fine powder obtained in the step 1 with water, uniformly stirring, respectively adding amylase, saccharifying enzyme and papain, boiling in water bath, inactivating enzyme, and centrifuging to obtain burdock fibers;
step 3: preparing burdock fiber powder: washing and centrifuging the burdock fibers obtained in the step 2 for multiple times to remove trace impurities to obtain a fiber solution, and freeze-drying the fiber solution to obtain burdock fiber powder;
step 4: preparing burdock nanocellulose: dispersing the burdock fiber powder obtained in the step 3 in sodium citrate buffer solution containing xylanase and cellulase, boiling to inactivate enzyme after stirring, centrifuging after ultrasonic treatment, collecting precipitate, and washing the precipitate with absolute ethyl alcohol to obtain burdock nanocellulose;
step 5: homogenizing the burdock nanocellulose obtained in the step 4 and mung bean protein to form foam;
step 6: and (5) putting the foam obtained in the step (5) into a refrigerator for freeze drying to obtain aerogel.
Further, in the step 1, superfine grinding is carried out until the particle size is 20-40 mu m, and sieving is carried out through a 400-600 mesh sieve.
Further, in the step 2, burdock fine powder and water are mixed according to a mass ratio of 1:5 to obtain a mixed solution, and the mass ratio of the added amylase, saccharifying enzyme and papain to the mixed solution is (2-4): 3-5): 19-21): 12000.
Further, in the step 2, the burdock fibers are obtained by centrifugation for 10-25 min at the rotation speed of 5000 g/min.
Further, in the step 2, a magnetic stirrer is used for stirring uniformly.
Further, in the step 3, washing is performed by using water and ethanol.
Further, in the step 4, a 50 mM sodium citrate buffer solution containing 2000U/mL xylanase and 3000U/mL cellulase is used, burdock fiber powder and the sodium citrate buffer solution are prepared according to a mass ratio of 1:50, 600 r/min is carried out at 20-80 ℃, stirring is carried out for 18-30 h, boiling is carried out for 5-15 min for enzyme deactivation, 600W ultrasonic treatment is carried out for 1-3 h, and centrifugation is carried out for 5-15 min at 1000 g/min.
Further, in the step 5, burdock nanocellulose with a concentration of 1% and mung bean protein with a concentration of 10% are used.
Further, in the step 5, a homogenizer is used, and the homogenizing is carried out for 10-20 min at 8000rpm to form foam.
Further, the temperature of the refrigerator in the step 6 is minus 60 to minus 100 ℃.
The beneficial effects are that: compared with the prior art, the aerogel taking the novel mung bean protein and burdock nanocellulose synergistically stabilized foam as the template has the following advantages: has high porosity, and plays a role in loading active substances and slow release. The burdock and mung bean food has high safety, green and healthy properties and high value. The burdock is economical and practical, has stable sources and is easy to realize industrialized development. The aerogel prepared by the method is a white spongy porous solid, the surface of the porous solid is distributed over nanoscale pore channels, the porous solid has high porosity, good adsorptivity and reusability, and the porous solid has a good slow release effect after the curcumin is loaded on the aerogel.
Drawings
FIG. 1 is a schematic diagram of a homogeneous foam of an embodiment of the present invention;
FIG. 2 is a graph of aerogel preparation of an embodiment of the present invention;
FIG. 3 is an aerogel scanning electron microscope of an embodiment of the present invention;
FIG. 4 is a diagram of the finished curcumin oleogel preparation of an embodiment of the invention;
fig. 5 is a simulated hydrolysis diagram of in vitro digestion of curcumin aerogel of an embodiment of the invention.
Detailed Description
The present invention is further illustrated in the accompanying drawings and detailed description which are to be understood as being merely illustrative of the invention and not limiting of its scope, and various modifications of the invention, which are equivalent to those skilled in the art upon reading the invention, will fall within the scope of the invention as defined in the appended claims.
An aerogel taking mung bean protein and burdock nanocellulose synergistically stabilized foam as a template comprises the following specific steps:
(1) Preparation of burdock nanocellulose
a: selecting burdock raw materials: selecting fresh burdock with the diameter of 4-6 cm as a raw material;
b: pretreatment of an extracted sample: cleaning burdock, drying, superfine grinding to a particle size of about 20-40 μm, and sieving with a 400-600 mesh sieve to obtain burdock fine powder;
c: preparing burdock nanocellulose: mixing the obtained 100 g burdock powder with 500 mL water, and uniformly dispersing by using a magnetic stirrer; 0.15g of alpha-amylase (60 ℃, pH 4.5) is added for 40 min,0.20 g of saccharifying enzyme (60 ℃, pH 6.0) is added for 40 min,1.0 g of papain (50 ℃, pH 6.0) is added for 60 min, and the enzyme is inactivated by boiling in water bath. Centrifuging at 5000 g/min for 5-20 min to obtain burdock fiber which is a light gray precipitate, washing and centrifuging with water and ethanol for multiple times to remove trace impurities to obtain fiber solution, and freeze-drying to obtain powdery fiber substance. Dispersing 5g burdock fiber powder in a sodium citrate buffer (50 mM, pH 4.8) 250 mL containing xylanase (2000U/mL) and cellulase (3000U/mL), and stirring at 50 ℃ for 18-30 h at 600 r/min; then boiling the sample for 5-15 min to inactivate enzyme, performing 600W ultrasonic treatment for 1-3 h, centrifuging at 1000 g/min for 5-15 min, collecting precipitate, and repeating twice; washing the precipitate with absolute ethyl alcohol, repeating twice, and collecting the precipitate, namely the burdock nanocellulose separated from the burdock fibers.
(2) Homogenizing the burdock nanocellulose obtained in the step (1) for 15min with 1% concentration and 10% concentration mung bean protein by using a homogenizer at 8000rpm, wherein abundant foam can be formed after homogenization;
(3) And (3) placing the foam obtained in the step (2) into a refrigerator at the temperature of minus 60 ℃ to minus 100 ℃ and freeze-drying to obtain aerogel.
Example 1
(1) Preparation of burdock nanocellulose
a: selecting burdock raw materials: selecting fresh burdock with the diameter of 5cm as a raw material;
b: pretreatment of an extracted sample: cleaning burdock, oven drying, micronizing to particle size of about 30 μm, and sieving with 600 mesh sieve to obtain burdock fine powder;
c: preparing burdock nanocellulose: mixing the obtained 100 g burdock powder with 500 mL water, and uniformly dispersing by using a magnetic stirrer; 0.15g of alpha-amylase (60 ℃, pH 4.5) is added for 40 min,0.20 g of saccharifying enzyme (60 ℃, pH 6.0) is added for 40 min,1.0 g of papain (50 ℃, pH 6.0) is added for 60 min, and the enzyme is inactivated by boiling in water bath. Centrifuging at 5000 g/min for 10 min to obtain burdock fiber as light gray precipitate, washing with water and ethanol for several times, centrifuging to remove trace impurities to obtain fiber solution, and lyophilizing to obtain powdery fiber material. Dispersing 5g burdock fiber powder in 250 mL sodium citrate buffer (50 mM, pH 4.8) containing xylanase (2000U/mL) and cellulase (3000U/mL), stirring at 50deg.C for 600 r/min, and stirring for 24 h; then boiling the sample for 10 min to inactivate enzyme, performing 600W ultrasonic treatment for 2.0 and h, centrifuging at 1000 g/min for 10 min, collecting precipitate, and repeating twice; washing the precipitate with absolute ethyl alcohol, repeating twice, and collecting the precipitate, namely the burdock nanocellulose separated from the burdock fibers.
(2) As shown in fig. 1, the obtained burdock nanocellulose was homogenized for 15min with 1% concentration and 10% concentration of mung bean protein using a homogenizer at 8000rpm, and after homogenization, abundant foam was formed, and the foam was maintained at 47 h at normal temperature.
(3) As shown in FIG. 2, the foam formed by homogenizing mung bean protein and burdock nanocellulose is put into a refrigerator at-80 ℃ and freeze-dried to prepare aerogel. An electron micrograph of the aerogel is shown in figure 3.
(4) As shown in fig. 4, under the heating condition of 80 ℃, corn oil with the loading amount of 1% curcumin is dissolved, corn oil with curcumin dissolved is adsorbed by aerogel, and the mass ratio of curcumin oil solution to aerogel is 5:1, so that the curcumin-loaded oleogel is obtained.
In vitro digestion simulation of curcumin loaded oleogel results are shown in figure 5. Configuration of gastric digestive juice: 2 mg/mL NaCl, 7 mL/L HCI and 3.2 mg/mL pepsin, wherein all the components are prepared by ultrapure water; configuration of intestinal juice: salt solution (36.7. 36.7 mg/mL CaC 1) 2 . H 2 O, 218.7 mg/mL NaCI), 24 mg/mL lipase, 54 mg/mL bile salts, wherein the saline solution was prepared with ultrapure water and the lipase and bile salts were prepared with 5 mmol/L PBS (pH 7.0). Preheating gastric digestion solution at 37deg.C for 5min to obtain 15 ml, adding curcumin oleogel 15g, rapidly adjusting pH of the mixed system to 2.5 with 1 mol/L NaOH, digesting 2 h in a water bath (100 r/min) with constant temperature shaking table at 37deg.C, and rapidly adjusting pH of the mixed system to 7.0 with 2 mol/L NaOH after gastric digestion. The intestinal simulated solution was preheated at 37℃for 5min, 1.5. 1.5 mL of saline solution, 3.5mL of bile salt solution and 2.5mL of lipase solution were sequentially added to the above gastric digestion solution, the pH of the mixed system was adjusted to 7.0 with 1 mol/L NaOH, and finally digested at 37℃in a thermostatic shaker water bath (100 r/min) for 2 h. During this time, naOH was used to maintain the pH of the mixed system at 7.0. The amount of fat in the sample that was hydrolysed to free fatty acids by the action of lipase during in vitro simulated digestion was measured.
FFA release rate (%) =100×c NaOH ×V NaOH ×M×2W
Wherein C is NaOH The molar concentration of the NaOH solution used for titration;
m is the mass fraction of corn oil (872, g/mol);
w is the weight (g) of corn oil during digestion.
Therefore, the aerogel can achieve better slow release effect, has similar effect as the ordinary aerogel, but has lower cost.
Claims (6)
1. An aerogel taking mung bean protein and burdock nanocellulose synergistically stabilized foam as a template is characterized in that: the method comprises the following steps:
step 1: pretreatment of raw materials: selecting fresh burdock as a raw material, cleaning, drying, ultrafine grinding and sieving to obtain burdock fine powder;
step 2: preparing burdock fibers: mixing the burdock fine powder obtained in the step 1 with water, uniformly stirring, respectively adding amylase, saccharifying enzyme and papain, boiling in water bath, inactivating enzyme, and centrifuging to obtain burdock fibers;
step 3: preparing burdock fiber powder: washing and centrifuging the burdock fibers obtained in the step 2 for multiple times to remove trace impurities to obtain a fiber solution, and freeze-drying the fiber solution to obtain burdock fiber powder;
step 4: preparing burdock nanocellulose: dispersing the burdock fiber powder obtained in the step 3 in sodium citrate buffer solution containing xylanase and cellulase, boiling to inactivate enzyme after stirring, centrifuging after ultrasonic treatment, collecting precipitate, and washing the precipitate with absolute ethyl alcohol to obtain burdock nanocellulose;
step 5: homogenizing the burdock nanocellulose obtained in the step 4 and mung bean protein to form foam;
step 6: putting the foam obtained in the step 5 into a refrigerator for freeze drying to obtain aerogel;
in the step 2, burdock fine powder and water are mixed according to a mass ratio of 1:5 to obtain a mixed solution, and the mass ratio of amylase, saccharifying enzyme, papain and the mixed solution is (2-4): (3-5): (19-21): 12000;
in the step 4, 50 mM sodium citrate buffer containing 2000U/mL xylanase and 3000U/mL cellulase is used, and burdock fiber powder and the sodium citrate buffer are mixed according to a ratio of 1:50, stirring for 18-30 h at the temperature of 20-80 ℃ at 600 r/min, boiling for 5-15 min to inactivate enzyme, performing ultrasonic treatment at 600W for 1-3 h, and centrifuging for 5-15 min at 1000 g/min;
in the step 5, burdock nanocellulose with the concentration of 1% and mung bean protein with the concentration of 10% are used.
2. The aerogel with mung bean protein and burdock nanocellulose synergistically stabilized foam as templates according to claim 1, wherein: and (3) superfine grinding is carried out in the step (1) until the particle size is 20-40 mu m, and sieving with a 400-600 mesh sieve.
3. The aerogel with mung bean protein and burdock nanocellulose synergistically stabilized foam as templates according to claim 1, wherein: and in the step 2, centrifuging for 10-25 min at the rotation speed of 5000 g/min to obtain the burdock fibers.
4. The aerogel with mung bean protein and burdock nanocellulose synergistically stabilized foam as templates according to claim 1, wherein: and in the step 2, a magnetic stirrer is used for stirring uniformly, and in the step 3, water and ethanol are used for washing.
5. The aerogel with mung bean protein and burdock nanocellulose synergistically stabilized foam as templates according to claim 1, wherein: and in the step 5, a homogenizer is used, and the homogenization is carried out for 10-20 min at 8000rpm to form foam.
6. The aerogel with mung bean protein and burdock nanocellulose synergistically stabilized foam as templates according to claim 1, wherein: the temperature of the refrigerator in the step 6 is minus 60 to minus 100 ℃.
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