CN115521884B - Paenibacillus HNP12 and application thereof - Google Patents
Paenibacillus HNP12 and application thereof Download PDFInfo
- Publication number
- CN115521884B CN115521884B CN202210857304.4A CN202210857304A CN115521884B CN 115521884 B CN115521884 B CN 115521884B CN 202210857304 A CN202210857304 A CN 202210857304A CN 115521884 B CN115521884 B CN 115521884B
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- paenibacillus
- hnp12
- soil
- acid
- improving
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- 241000179039 Paenibacillus Species 0.000 title claims abstract description 37
- 239000002689 soil Substances 0.000 claims abstract description 48
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 24
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000011591 potassium Substances 0.000 claims abstract description 14
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 14
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000011574 phosphorus Substances 0.000 claims abstract description 13
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 13
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 12
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000011575 calcium Substances 0.000 claims abstract description 12
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 12
- 229910052742 iron Inorganic materials 0.000 claims abstract description 12
- 239000011777 magnesium Substances 0.000 claims abstract description 12
- 229910052749 magnesium Inorganic materials 0.000 claims abstract description 12
- 244000005700 microbiome Species 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims description 22
- 230000004151 fermentation Effects 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 9
- 238000002791 soaking Methods 0.000 claims description 9
- 238000009472 formulation Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000003337 fertilizer Substances 0.000 abstract description 20
- 239000002253 acid Substances 0.000 abstract description 17
- 241000750042 Vini Species 0.000 abstract description 12
- 239000003513 alkali Substances 0.000 abstract description 12
- 235000015097 nutrients Nutrition 0.000 abstract description 9
- 239000000126 substance Substances 0.000 abstract description 9
- 230000001580 bacterial effect Effects 0.000 description 15
- 239000001888 Peptone Substances 0.000 description 13
- 108010080698 Peptones Proteins 0.000 description 13
- 235000015278 beef Nutrition 0.000 description 13
- 235000019319 peptone Nutrition 0.000 description 13
- 238000011282 treatment Methods 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 9
- 230000012010 growth Effects 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 240000003768 Solanum lycopersicum Species 0.000 description 7
- 230000002053 acidogenic effect Effects 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 235000015193 tomato juice Nutrition 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- XJRPTMORGOIMMI-UHFFFAOYSA-N ethyl 2-amino-4-(trifluoromethyl)-1,3-thiazole-5-carboxylate Chemical compound CCOC(=O)C=1SC(N)=NC=1C(F)(F)F XJRPTMORGOIMMI-UHFFFAOYSA-N 0.000 description 2
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- IWYDHOAUDWTVEP-UHFFFAOYSA-N mandelic acid Chemical compound OC(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-N 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 235000019260 propionic acid Nutrition 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 1
- 239000001124 (E)-prop-1-ene-1,2,3-tricarboxylic acid Substances 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241000193749 Bacillus coagulans Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- HEBKCHPVOIAQTA-IMJSIDKUSA-N L-arabinitol Chemical compound OC[C@H](O)C(O)[C@@H](O)CO HEBKCHPVOIAQTA-IMJSIDKUSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 229930182816 L-glutamine Natural products 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229940091181 aconitic acid Drugs 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 229960003767 alanine Drugs 0.000 description 1
- DLHONNLASJQAHX-UHFFFAOYSA-N aluminum;potassium;oxygen(2-);silicon(4+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Al+3].[Si+4].[Si+4].[Si+4].[K+] DLHONNLASJQAHX-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229960003121 arginine Drugs 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 229940054340 bacillus coagulans Drugs 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- QVYARBLCAHCSFJ-UHFFFAOYSA-N butane-1,1-diamine Chemical compound CCCC(N)N QVYARBLCAHCSFJ-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- GTZCVFVGUGFEME-IWQZZHSRSA-N cis-aconitic acid Chemical compound OC(=O)C\C(C(O)=O)=C\C(O)=O GTZCVFVGUGFEME-IWQZZHSRSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229960002743 glutamine Drugs 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 1
- 239000010445 mica Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229960001790 sodium citrate Drugs 0.000 description 1
- 229960004025 sodium salicylate Drugs 0.000 description 1
- 229940074404 sodium succinate Drugs 0.000 description 1
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- GTZCVFVGUGFEME-UHFFFAOYSA-N trans-aconitic acid Natural products OC(=O)CC(C(O)=O)=CC(O)=O GTZCVFVGUGFEME-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 description 1
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Fertilizers (AREA)
Abstract
The paenibacillus HNP12 disclosed by the invention is preserved in the microorganism strain collection center (GDMCC) of Guangdong province in 2022 and 06 month 02, the strain collection number is GDMCC NO.62513, and the class is named as paenibacillus ((Paenibaciplus vini). The paenibacillus HNP12 for fermenting and producing acid provided by the invention can convert the fixed nutrients such as phosphorus, potassium, iron, magnesium, calcium and the like in the saline-alkali soil into effective states by adjusting the pH value of the saline-alkali soil, so that the nutrients are absorbed and utilized by crops, the application amount of chemical fertilizer is reduced, and the fertilizer utilization rate is improved.
Description
Technical Field
The invention relates to the technical field of biology, in particular to bacillus species HNP12 and application thereof.
Background
Acid-producing bacteria have wide application in the fields of food, chemical industry and the like. Acetic acid bacteria for producing vinegar, lactic acid bacteria for fermenting pickle and dairy products, yeast for producing citric acid by fermentation, and the like. The documents related to the acid production of bacillus are also reported, such as bacillus coagulans, lactobacillus and the like, but the documents related to the acid production of paenibacillus are rarely reported, and particularly, the paenibacillus with stronger acid production capability is not reported.
Nitrogen, phosphorus and potassium are three essential nutrient elements for plant growth, and the contents of available phosphorus and quick-acting potassium which can be directly absorbed and utilized by crops in most of the soil in China are low. In recent years, along with the mass abuse of chemical fertilizers, excessive phosphate in the chemical fertilizers is easy to form phosphate which is difficult to be absorbed and utilized by crops, such as iron, magnesium, calcium and other trace elements in soil, and excessive potassium in the chemical fertilizers is easy to exist in potassium feldspar and mica in a mineral form, so that the crops are difficult to absorb and utilize. In the past, a large amount of fertilizer is applied, so that not only is the soil structure destroyed, but also the problem of soil hardening is increasingly serious, the utilization rate of the fertilizer is reduced, and the fertilizer cost is increased year by year. Through the action of microorganisms, the pH value of the soil is regulated to convert nutrients such as phosphorus, potassium, iron, calcium, magnesium and the like which are difficult to be absorbed and utilized by crops in the soil into an effective state for the crops to directly absorb and utilize, so that the application amount of chemical fertilizers can be reduced, the fertilizer utilization rate can be improved while the yield is ensured, the fertilizer cost is saved, and the method has very important significance.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the problems in the related art. Therefore, the invention aims to provide the paenibacillus HNP12 for fermenting and producing acid, which can convert the fixed nutrients such as phosphorus, potassium, iron, magnesium, calcium and the like in the saline-alkali soil into effective states for crop absorption and utilization by adjusting the pH value of the saline-alkali soil, reduce the application amount of chemical fertilizers and improve the utilization rate of the chemical fertilizers.
In order to achieve the above purpose, the present application adopts the following technical scheme: paenibacillus HNP12 of the invention has been deposited in the microorganism strain collection (GDMCC) of Guangdong province on the year 06 month 02 of 2022, the strain collection number is GDMCC NO.62513, the class name is Paenibacillus ((Paenibaciplus vini), and the deposit address is building 5 of No. 59 of 100 university of Mitsui in Guangzhou, city.
The application provides application of paenibacillus (Paenibaciplus vini) HNP12 in the field of improving soil pH value.
The application provides application of paenibacillus (Paenibaciplus vini) HNP12 in the field of improving element content in soil.
The application provides a preparation for improving the pH value of soil, comprising paenibacillus (Paenibaciplus vini) HNP12.
The application provides a preparation for improving element content in soil, comprising paenibacillus (Paenibaciplus vini) HNP12.
The application provides a method for improving the pH value of soil, which comprises the step of soaking the soil by using fermentation liquor of paenibacillus (Paenibaciplus vini) HNP12.
The application provides a method for improving element content in soil, which comprises the step of soaking the soil by using fermentation liquor of paenibacillus (Paenibaciplus vini) HNP12.
Compared with the prior art, the technical scheme provided by the embodiment of the application has the following advantages: the paenibacillus HNP12 provided by the application has the advantages of strong acid production capacity, high growth speed, strong environmental adaptability and strong stress tolerance. The strain is used for fermenting and producing acid, the pH value of the saline-alkali soil is regulated, and the fixed nutrients such as phosphorus, potassium, iron, calcium, magnesium and the like in the soil are converted into effective states which can be directly absorbed and utilized by crops, so that the utilization rate of the fertilizer is improved, and the application amount of the fertilizer is reduced.
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention. Modifications and substitutions to methods, procedures, or conditions of the present invention may be made without departing from the spirit and nature of the invention and are intended to be within the scope of the present invention. Unless otherwise indicated, the experimental materials, reagents, instruments, etc. used in the examples of the present invention are commercially available; all technical means in the embodiments of the present invention are conventional means well known to those skilled in the art unless specifically indicated.
The following media components of the present application are shown below:
modified beef extract peptone medium: 20g of glucose, 15g of peptone, 5g of sodium chloride, 0.5g of beef extract, 1000mL of distilled water and 20g of agar.
Acid-producing strain screening medium: 10g of glucose, 10g of starch, 15g of peptone, 5g of sodium chloride, 0.5g of beef extract, 3-4 drops of bromocresol purple indicator, 1000mL of distilled water and 20g of agar; the pH of the acid-producing strain screening medium is 7.1-7.3,
acid-producing strain fermentation broth: 10g of glucose, 10g of starch, 15g of peptone, 5g of sodium chloride, 0.5g of beef extract, 3-4 drops of bromocresol purple indicator and 1000mL of distilled water; the pH of the fermentation broth of the acid-producing strain is 7.1-7.3.
Modified beef extract peptone liquid medium: 10g of glucose, 10g of starch, 15g of peptone, 5g of sodium chloride, 0.5g of beef extract, 1000mL of distilled water and the pH of the modified beef extract peptone liquid medium is 7.1-7.3.
EXAMPLE 1 isolation and purification of strains
In the embodiment, the modified beef extract peptone culture medium is used for separating and purifying the strain of the tomato sample which is collected in tomato plant bases in Panzhihua city of Sichuan province and is putrefactive, odorous and sour. The method comprises the following specific steps:
30 parts of a tomato sample which is derived from a tomato planting base in Panzhihua city of Sichuan province and is putrefactive, odorous and sour are collected. After washing the tomato sample with distilled water, the juice of the tomato sample was squeezed out with a sterilized clip, and the squeezed tomato juice was then placed in a sterilized beaker. The resulting 30 tomato juice samples were then diluted to 10 with sterile water -6 After doubling. 20. Mu.L of the diluted tomato juice samples were pipetted into a sterile operating table and spread evenly on a modified beef extract peptone medium plate, 3 replicates per tomato juice sample. The uniformly coated 90 plates were then cultured in an incubator at 37 ℃. And (3) observing the growth condition of the thalli, picking out the bacterial colony with better growth vigor and different characteristics, and repeatedly subculturing by using a plate streaking method until the shape, color, texture and transparency of the bacterial colony are consistent. Finally, the morphology is further observed through simple dyeing and microscopic examination, and the dyeing condition is unified as a strain purification standard with uniform length and width.
And (3) preserving: culturing the separated and purified strain on a modified beef extract peptone culture medium plate for 1-2d, and collecting thalli on the plate. Stored in 15% sterilized glycerol (-20 ℃).
Screening: activating the separated and purified strain, and performing strain acidogenesis qualitative test to obtain a strain with concentration of 10 with sterile water 8 cell/mL bacterial suspension is absorbed to 10 mu L of bacterial suspension point-planted on an acidogenic strain screening culture medium, and after 24 hours of culture in a bacterial incubator at 37 ℃, the bacterial suspension is screened out, the bacterial suspension with the color of the culture medium changed from purple to yellow is totally screened out to 92 acidogenic strains, and the bacterial suspension point-planted on the acidogenic strain is named FQ01-FQ92. Then the primary screening is carried out to obtain92 acidogenic strains FQ01-FQ92 of (1) are respectively inoculated into an acidogenic strain fermentation liquid culture medium, and after shaking culture is carried out for 48 hours at 37 ℃, the pH value of a fermentation liquid is measured, so that the strain HNP12 with more viable bacteria and the strongest acidogenic capacity is finally obtained, the pH value of the fermentation liquid is reduced to 4.0, and a fermentation product is measured and analyzed by a gas-phase mass spectrometer, wherein the product contains organic acids such as acetic acid, propionic acid, butyric acid, citric acid and the like.
Example 2 physiological and Biochemical Properties and Classification of strains
The paenibacillus HNP12 has the following morphological, physiological and biochemical characteristics:
a. characteristics of bacterial cell shape
The bacillus cells are rod-shaped and can produce spores and gram-positive bacteria.
b. Colony morphology characterization
The growth speed of the beef extract peptone culture medium plate is high, the bacterial colony is round and convex (the growth is carried out for 24 hours at 37 ℃), the bacterial colony is not transparent in beige color, and the bacterial colony diameter is 2-5 mm. The optimal growth temperature is 37 ℃ and the optimal growth pH value is 5-6.
c. Physiological and biochemical characteristics
The preparation method is facultative anaerobic, the methyl red reaction is positive, the V.P reaction is negative, the catalase is positive, the indole reaction is positive, the auxin IAA can be secreted, the gelatin can be liquefied, and the starch hydrolysis is positive. The paenibacillus HNP12 can produce acid on the modified beef extract peptone liquid medium, after fermentation for 48 hours, the pH value of the fermentation liquid can be reduced to 4.0, and the fermentation product contains organic acids such as acetic acid, propionic acid, butyric acid, citric acid and the like. Can grow on a culture medium taking vanillic acid, histidine, dextrin, aconitic acid, butanediamine, hydroxyphenylacetic acid, gamma-aminobutyric acid, L-glutamine, sodium citrate, inosine, L-alanine, arginine, L-arabitol, sodium succinate, aspartic acid, D-xylose, sodium salicylate, tyrosine, proline and phenylalanine as carbon sources; the pH growth range is wider, and the pH can be resisted between 2 and 8; the concentration of NaCl resistance can reach 7 percent.
The 16S rDNA sequence of Paenibacillus HNP12 is shown in the appendix.
The invention has the determination of the molecular classification status of the paenibacillus HNP12 with stronger acid production capacity:
the bacterial 16S rDNA gene universal primers 25f and 1492r are adopted for amplification, the PCR product is directly subjected to sequence determination, the obtained DNA sequence is input into GenBank for comparison, and the position of the bacterial strain species of the paenibacillus (Paenibaciplus vini, HNP 12) in the taxonomy is preliminarily determined. As a result, it was found that the Paenibacillus of the present invention belongs to Paenibacillus genus (Paenibacillus) and has a similarity of 99.4% with the Paenibacillus (Paenibaciplus vini, HNP 12) model strain FW 100M-2.
In combination with the above physiological and biochemical characteristics, 16S rDNA sequence analysis, paenibacillus (Paenibaciplus vini, HNP 12) of the present invention belongs to Paenibacillus genus.
Example 3 use of strains in improving soil
Inoculating the strain HNP12 into an acidogenic strain fermentation liquid culture medium, and reducing the pH value of the fermentation liquid to between 4.0 and 4.2 after shake cultivation for 48 hours at 37 ℃.
Collecting soil with serious soil hardening and soil saline-alkali degree caused by long-term application of chemical fertilizer, filtering the collected saline-alkali soil by using a 60-mesh filter screen, uniformly dividing the obtained soil into 9 parts, ensuring that the characters of each part of saline-alkali soil are consistent, and taking 500g of each part of saline-alkali soil for test, wherein the initial pH value of the soil is 8.0. And (3) soaking a saline-alkali soil by using a bacterial strain fermentation liquid for testing: 3 treatments were performed and 3 replicates were performed. The strain HNP12 is inoculated in a modified beef extract peptone liquid culture medium for fermentation for 48 hours, and the fermentation product is obtained for the following treatment.
A1: sterile culture solution; a2, fermenting liquid; a3: and (5) clean water. And 3 treatments are carried out on the soil in the saline-alkali soil until 500g of the soil is completely soaked and submerged, the soil in the sample and the soaking liquid are separated by using a large-scale centrifuge after soaking for 48 hours, and finally the pH value of the soaked soil and the contents of elements such as available phosphorus, quick-acting potassium, iron, calcium and magnesium in the soaking liquid are measured. The result shows that the pH value of the soil of the treatment group A2 is reduced to about 6.5; the pH values of the soil of the treatment group A1 and the soil of the treatment group A3 are not greatly different, and the pH values of the two are about 7.6. The content of the effective phosphorus, the quick-acting potassium, the iron, the calcium, the magnesium and other elements in the soaking liquid are measured, and the result shows that the content of each nutrient in the treatment group A2 is higher than that in the treatment group A1 and the treatment group A3, wherein the content of each nutrient in the treatment group A1 is slightly higher than that in the treatment group A3, but the content of each nutrient does not reach the level of obvious difference, and compared with the treatment group A3, the effective phosphorus content in the soaking liquid is increased by 67.2%, the quick-acting potassium content is increased by 34.5%, the iron content is increased by 17.1%, the magnesium content is increased by 13.1%, and the calcium content is increased by 23.9%.
Paenibacillus (Paenibaciplus vini) HNP12 for producing acid by fermentation can produce acid under different fermentation conditions. The fertilizer is derived from the tomatoes which are putrefactive, smelly and sour, the pH value of the saline-alkali soil can be adjusted through fermentation and acid production, and the fixed nutrients such as phosphorus, potassium, iron, calcium, magnesium and the like in the soil can be converted into effective states which can be directly absorbed and utilized by crops, so that the fertilizer application amount is reduced, the fertilizer utilization rate is improved, and the fertilizer has important significance in agricultural production.
It is to be understood that the above examples only represent preferred embodiments of the present invention, which are described in more detail and are not to be construed as limiting the scope of the invention; it should be noted that, for a person skilled in the art, the above technical features can be freely combined, and several variations and modifications can be made without departing from the scope of the invention; therefore, all changes and modifications that come within the meaning and range of equivalency of the claims are to be embraced within their scope.
Claims (7)
1. A paenibacillus HNP12, wherein paenibacillus HNP12 was deposited with the collection of microorganism strains, canton province, under accession number GDMCC No.62513, at month 2 of 2022.
2. The use of Paenibacillus HNP12 according to claim 1 for improving the pH of soil.
3. The application of the paenibacillus HNP12 in the field of improving the content of phosphorus, potassium, iron, calcium and magnesium elements in soil.
4. A formulation for improving soil pH comprising paenibacillus HNP12 according to claim 1.
5. A formulation for improving the content of phosphorus, potassium, iron, calcium and magnesium in soil, comprising paenibacillus HNP12 according to claim 1.
6. A method for improving the pH value of soil, which is characterized in that the fermentation liquid of paenibacillus HNP12 in claim 1 is used for soaking the soil.
7. A method for improving the content of phosphorus, potassium, iron, calcium and magnesium elements in soil is characterized in that the fermentation liquor of paenibacillus HNP12 in claim 1 is adopted to soak the soil.
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| CN104560789A (en) * | 2014-12-13 | 2015-04-29 | 郑州市污水净化有限公司 | Peanut growth promoting rhizobacteria HS2 and application thereof |
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| CN104126003A (en) * | 2012-02-17 | 2014-10-29 | 医药中心有限公司 | Recombinant escherichia coli strains |
| CN104560789A (en) * | 2014-12-13 | 2015-04-29 | 郑州市污水净化有限公司 | Peanut growth promoting rhizobacteria HS2 and application thereof |
| CN110613849A (en) * | 2018-06-20 | 2019-12-27 | 珠海岐微生物科技有限公司 | Traditional Chinese medicine composition and application thereof in regulating intestinal microorganisms |
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