CN115521781B - High-fluorescence-performance carbon quantum dot for detecting oxytocin and preparation method thereof - Google Patents
High-fluorescence-performance carbon quantum dot for detecting oxytocin and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a high-fluorescence carbon quantum dot for detecting oxytocin, and a preparation method and application thereof, wherein the preparation method comprises the following steps: uniformly mixing a carbon source, a nitrogen source and deionized water, and then carrying out hydrothermal reaction to obtain a reaction solution; after cooling, the carbon quantum dots with high fluorescence performance for detecting oxytocin are prepared by filtering, dialyzing and freeze-drying in sequence. The nitrogen-doped yellowish green fluorescent carbon quantum dot obtained by the hydrothermal reaction has good fluorescent property and excellent water solubility, is used for measuring oxytocin, and has the advantages of simplicity, convenience and rapidness in operation, low detection limit and high accuracy. The invention relates to the technical field of medicine content detection, and solves the problems of complex operation and low accuracy of a method for detecting the oxytocin content in the prior art.
Description
Technical Field
The invention relates to the technical field of drug content detection, in particular to a high-fluorescence carbon quantum dot for detecting oxytocin and a preparation method thereof.
Background
The oxytocin is neuropeptide (Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-HN 2) composed of 9 amino acids, can promote delivery and milk secretion, is prepared by extracting pig pituitary gland or total synthesis, and can be clinically used for inducing labor, hastening parturition, postpartum and uterine bleeding caused by uterine contractile debilitation or dysplasia after abortion. Currently, the assay methods of oxytocin mainly include a biological activity assay method, an HPLC method, an LC/MS method and an immunoassay method; wherein, the biological activity measuring method has complex operation, and the measuring result is easily influenced by individual differences of animals and human operation errors; HPLC and LC/MS require instrument support, and are long in time consumption and high in cost; immunoassays are rapid and sensitive, but are not highly accurate. Thus, there is a strong need to establish a rapid, sensitive and accurate analytical assay for oxytocin concentration in oxytocin materials, formulations or clinically useful drugs.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide the carbon quantum dot with high fluorescence performance for detecting the oxytocin, and the preparation method and the application thereof, so as to solve the problems of complex operation and low accuracy of the method for detecting the oxytocin content in the prior art.
The technical scheme for solving the technical problems is as follows: the preparation method of the high-fluorescence carbon quantum dot for detecting oxytocin comprises the following steps:
(1) Uniformly mixing a carbon source, a nitrogen source and deionized water, and then carrying out hydrothermal reaction to obtain a reaction solution;
(2) And (3) cooling the reaction solution prepared in the step (1), and sequentially filtering, dialyzing and freeze-drying to prepare the carbon quantum dots with high fluorescence performance for detecting the oxytocin.
The beneficial effects of the invention are as follows: the nitrogen-doped yellowish green fluorescent carbon quantum dot obtained by the hydrothermal reaction has good fluorescent property and excellent water solubility, is used for measuring oxytocin, and has the advantages of simplicity, convenience and rapidness in operation, low detection limit and high accuracy.
Based on the technical scheme, the invention can also be improved as follows:
further, in the step (1), the molar volume ratio of the carbon source, the nitrogen source and the deionized water is 0.5mmol:0.25-2mmol:10-25mL.
Further, in the step (1), the carbon source is quercetin.
Further, in the step (1), the nitrogen source is o-phenylenediamine.
Further, in the step (1), the hydrothermal reaction is carried out for 4-8 hours at 160-240 ℃.
Further, in the step (2), the reaction solution is filtered through a membrane having a pore size of 0.2 to 0.24. Mu.m.
Further, in the step (2), the reaction solution was filtered through a membrane having a pore size of 0.22. Mu.m.
Further, in step (2), the solution is dialyzed for 12 to 48 hours under the condition that the molecular weight cut-off is 1000 to 5000 Da.
The invention also provides the carbon quantum dot prepared by the preparation method for detecting the high-fluorescence-performance carbon quantum dot by using the oxytocin.
The invention also provides application of the carbon quantum dot with high fluorescence performance in detecting the content of the oxytocin.
A detection method of the content of the oxytocin comprises the following steps:
(1) Mixing the carbon quantum dots with high fluorescence performance for detecting the oxytocin with water to prepare a carbon quantum dot solution;
(2) Respectively adding different concentrations of oxytocin standard solutions into the carbon quantum dot solution prepared in the step (1), and respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after the standard solution is added by taking 460nm as an excitation wavelength to obtain a fluorescence intensity change value of the carbon quantum dot solution after the standard solution is added relative to the carbon quantum dot solution before the standard solution is added at the maximum emission wavelength;
(3) Drawing a standard curve according to the change value of the fluorescence intensity obtained in the step (2) and the concentration of the corresponding oxytocin standard solution to obtain a linear equation;
(4) And (3) adding the to-be-detected oxytocin solution into the carbon quantum dot solution prepared in the step (1), respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after adding the to-be-detected oxytocin solution by taking 460nm as an excitation wavelength to obtain a fluorescence intensity change value of the carbon quantum dot solution after adding the to-be-detected oxytocin solution relative to the carbon quantum dot solution before adding the to-be-detected oxytocin solution at the maximum emission wavelength, and substituting the fluorescence intensity change value into a linear equation to obtain the concentration of the to-be-detected oxytocin solution.
Further, in the step (1), the concentration of the carbon quantum dot solution is 0.05-1.5mg/mL.
Further, in the step (1), the concentration of the carbon quantum dot solution is 0.5mg/mL.
Further, in the step (2), the volume ratio of the carbon quantum dot solution to the oxytocin standard solution is 1:0.5-2.
Further, in the step (2), the volume ratio of the carbon quantum dot solution to the oxytocin standard solution is 1:1.
further, in step (2), the concentrations of the different oxytocin standard solutions are 0.2IU/mL, 0.36IU/mL, 0.4IU/mL, 0.6IU/mL, 0.8IU/mL, 1.2IU/mL, 1.6IU/mL, 2IU/mL, 3IU/mL, 4IU/mL, 5IU/mL, 6IU/mL, 7IU/mL, 8IU/mL, 9IU/mL and 10IU/mL, respectively.
Further, in the step (3), the concentration of the oxytocin standard solution is in the range of 0.2-5IU/mL, and the linear equation is y=29298x+17654, R 2 =0.9957, where y is the variation of fluorescence intensity, x is the concentration of the oxytocin standard solution, and R is the linear fitting constant.
Further, in the step (3), the concentration of the oxytocin standard solution is linearly in the range of 5-10IU/mLThe process is y=6894x+127986, r 2 = 0.9928, where y is the variation of fluorescence intensity, x is the concentration of the oxytocin standard solution, and R is the linear fitting constant.
Further, in the step (4), the detection limit of oxytocin is 0.0196IU/mL.
Further, the detection limit LOD is calculated by the following method: repeatedly measuring the fluorescence intensity of the carbon quantum dot solution before adding the standard solution in the step (2) for 11 times, calculating the standard deviation of the blank fluorescence intensity, obtaining the slope of the standard curve according to the standard curve in the step (3), and calculating the detection limit LOD by using the following formula according to the signal-to-noise ratio S/N=3:
lod=3×standard deviation of blank fluorescence intensity/standard curve slope
The invention has the following beneficial effects:
1. the carbon source adopted by the invention is quercetin, and a plurality of plants contain abundant quercetin, so that the carbon source is abundant in resources, low in cost and easy to obtain; the invention adopts water as a reaction solvent, is synthesized by a one-step hydrothermal method, has simple and convenient operation process, is environment-friendly, is economical and efficient, and has very important social significance.
2. Compared with the traditional sensing probe based on the organic dye, the characteristic of the carbon quantum dot as a fluorophore is superior, the problems of photobleaching and toxicity are avoided, the existence of functional groups such as surface hydroxyl groups, carboxyl groups, carbonyl groups, epoxy groups and the like can be endowed with the solubility of the carbon quantum dot in water, and meanwhile, the groups pave the way for the surface functionalization of the quantum dot and provide selectivity for target analytes.
3. The method takes the simple and easily obtained quercetin as the raw material to dope the polyamino micromolecules, prepares the carbon quantum dot with high fluorescence performance, and successfully uses the carbon quantum dot for measuring the content of the oxytocin.
Drawings
FIG. 1 is a high resolution transmission electron microscope image of the carbon quantum dots prepared in example 1;
FIG. 2 is a graph showing the particle size distribution of the carbon quantum dots prepared in example 1;
FIG. 3 is an infrared spectrum of the carbon quantum dots prepared in example 1;
FIG. 4 is an ultraviolet spectrum of the carbon quantum dots prepared in example 1;
FIG. 5 is a fluorescence spectrum of the carbon quantum dots prepared in example 1;
FIG. 6 is a graph showing the photo stability of the carbon quantum dots prepared in example 1;
FIG. 7 is a graph showing the linear relationship between fluorescence intensity and oxytocin concentration (0.2-5 IU/mL) of the carbon quantum dots prepared in example 1;
FIG. 8 is a graph showing the linear relationship between fluorescence intensity and oxytocin concentration (5-10 IU/mL) of the carbon quantum dots prepared in example 1.
Detailed Description
The principles and features of the present invention are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1:
the preparation method of the carbon quantum dot for detecting the high fluorescence performance by using the oxytocin comprises the following steps:
(1) Uniformly mixing quercetin, o-phenylenediamine and deionized water, and performing hydrothermal reaction for 6 hours at 220 ℃ to obtain a reaction solution; wherein, the mol volume ratio of quercetin, o-phenylenediamine and deionized water is 0.5mmol:1.5mmol:20mL;
(2) And (3) cooling the reaction solution prepared in the step (1), filtering by a membrane with the pore diameter of 0.22 mu m, transferring the filtrate into a dialysis bag with the molecular weight cutoff of 2000Da, dialyzing for 24 hours, and freeze-drying to obtain the high-fluorescence carbon quantum dot for detecting oxytocin.
A detection method of the content of the oxytocin comprises the following steps:
(1) Mixing the prepared carbon quantum dots with high fluorescence performance for detecting oxytocin with water to prepare a carbon quantum dot solution with the concentration of 0.5 mg/mL;
(2) Taking 100 mu L of the carbon quantum dot solution prepared in the step (1), respectively adding 100 mu L of the different-concentration hysterin standard solution (0.2 IU/mL, 0.36IU/mL, 0.4IU/mL, 0.6IU/mL, 0.8IU/mL, 1.2IU/mL, 1.6IU/mL, 2IU/mL, 3IU/mL, 4IU/mL, 5IU/mL, 6IU/mL, 7IU/mL, 8IU/mL, 9IU/mL and 10 IU/mL), respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after the standard solution is added by taking 460nm as an excitation wavelength, and obtaining a fluorescence intensity change value of the carbon quantum dot solution at the maximum emission wavelength 542nm of the carbon quantum dot solution before and after the standard solution is added;
(3) Drawing a standard curve according to the change value of the fluorescence intensity obtained in the step (2) and the concentration of the corresponding oxytocin standard solution to obtain a linear equation;
wherein, the concentration of the oxytocin standard solution is in the range of 0.2-5IU/mL, the linear equation is y=29298x+176554, R 2 =0.9957, where y is the variation of fluorescence intensity, x is the concentration of the oxytocin standard solution, and R is the linear fitting constant (see fig. 7,F 0 The fluorescence intensity without adding oxytocin Gong Sushi is shown, and F is the fluorescence intensity when oxytocin is added);
the linear equation of the concentration of the oxytocin standard solution in the range of 5-10IU/mL is y=6894x+127986, R 2 0.9928 where y is the variation of fluorescence intensity, x is the concentration of the oxytocin standard solution, and R is the linear fitting constant (see fig. 8,F 0 The fluorescence intensity without adding oxytocin Gong Sushi is shown, and F is the fluorescence intensity when oxytocin is added);
(4) Taking 100 mu L of the carbon quantum dot solution prepared in the step (1), adding 100 mu L of the to-be-detected oxytocin solution (the known concentration is 1.67IU/mL, and measuring for 6 times in parallel), respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after adding the to-be-detected oxytocin solution by taking 460nm as an excitation wavelength, so as to obtain a fluorescence intensity change value of the carbon quantum dot solution after adding the to-be-detected oxytocin solution relative to the carbon quantum dot solution before adding the to-be-detected oxytocin solution at the maximum emission wavelength of 542nm, and substituting the fluorescence intensity change value into a linear equation to obtain the concentration of the to-be-detected oxytocin solution to be 1.69IU/mL.
Example 2:
the preparation method of the carbon quantum dot for detecting the high fluorescence performance by using the oxytocin comprises the following steps:
(1) Uniformly mixing quercetin, o-phenylenediamine and deionized water, and performing hydrothermal reaction for 8 hours at 160 ℃ to prepare a reaction solution; wherein, the mol volume ratio of quercetin, o-phenylenediamine and deionized water is 0.5mmol:0.25mmol:10mL;
(2) And (3) cooling the reaction solution prepared in the step (1), filtering by a membrane with the pore diameter of 0.22 mu m, transferring the filtrate into a dialysis bag with the molecular weight cutoff of 1000Da, dialyzing for 48 hours, and freeze-drying to obtain the high-fluorescence carbon quantum dot for detecting the oxytocin.
A detection method of the content of the oxytocin comprises the following steps:
(1) Mixing the prepared carbon quantum dots with high fluorescence performance for detecting oxytocin with water to prepare a carbon quantum dot solution with the concentration of 0.05 mg/mL;
(2) Taking 100 mu L of the carbon quantum dot solution prepared in the step (1), respectively adding 50 mu L of the different-concentration hysterin standard solution (0.2 IU/mL, 0.36IU/mL, 0.4IU/mL, 0.6IU/mL, 0.8IU/mL, 1.2IU/mL, 1.6IU/mL, 2IU/mL, 3IU/mL, 4IU/mL, 5IU/mL, 6IU/mL, 7IU/mL, 8IU/mL, 9IU/mL and 10 IU/mL), respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after the standard solution is added by taking 460nm as an excitation wavelength, and obtaining a fluorescence intensity change value of the carbon quantum dot solution at the maximum emission wavelength 542nm of the carbon quantum dot solution before and after the standard solution is added;
(3) Drawing a standard curve according to the change value of the fluorescence intensity obtained in the step (2) and the concentration of the corresponding oxytocin standard solution to obtain a linear equation;
wherein, the concentration of the oxytocin standard solution is in the range of 0.2-5IU/mL, the linear equation is y=733x+2235, R 2 = 0.9908, where y is the variation of fluorescence intensity, x is the concentration of the oxytocin standard solution, and R is the linear fitting constant;
the concentration of the oxytocin standard solution is in the range of 5-10IU/mL, the linear equation is y=1518x+31106, R 2 ＝09878 wherein y is the variation of fluorescence intensity, x is the concentration of a standard solution of oxytocin, and R is a linear fitting constant;
(4) Taking 100 mu L of the carbon quantum dot solution prepared in the step (1), adding 50 mu L of the to-be-detected oxytocin solution (the known concentration is 3.75IU/mL, and measuring for 6 times in parallel), respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after adding the to-be-detected oxytocin solution by taking 460nm as an excitation wavelength, so as to obtain a fluorescence intensity change value of the carbon quantum dot solution at the maximum emission wavelength 542nm of the to-be-detected oxytocin solution relative to the carbon quantum dot solution before adding the to-be-detected oxytocin solution, and substituting the fluorescence intensity change value into a linear equation to obtain the concentration of the to-be-detected oxytocin solution to be 3.7IU/mL.
Example 3:
the preparation method of the carbon quantum dot for detecting the high fluorescence performance by using the oxytocin comprises the following steps:
(1) Uniformly mixing quercetin, o-phenylenediamine and deionized water, and performing hydrothermal reaction for 4 hours at 240 ℃ to prepare a reaction solution; wherein, the mol volume ratio of quercetin, o-phenylenediamine and deionized water is 0.5mmol:2mmol:25mL;
(2) And (3) cooling the reaction solution prepared in the step (1), filtering by a membrane with the pore diameter of 0.22 mu m, transferring the filtrate into a dialysis bag with the molecular weight cutoff of 5000Da, dialyzing for 12 hours, and freeze-drying to obtain the high-fluorescence carbon quantum dot for detecting oxytocin.
A detection method of the content of the oxytocin comprises the following steps:
(1) Mixing the prepared carbon quantum dots with high fluorescence performance for detecting oxytocin with water to prepare a carbon quantum dot solution with the concentration of 1.5 mg/mL;
(2) Taking 50 mu L of the carbon quantum dot solution prepared in the step (1), respectively adding 100 mu L of the different-concentration hysterin standard solution (0.2 IU/mL, 0.36IU/mL, 0.4IU/mL, 0.6IU/mL, 0.8IU/mL, 1.2IU/mL, 1.6IU/mL, 2IU/mL, 3IU/mL, 4IU/mL, 5IU/mL, 6IU/mL, 7IU/mL, 8IU/mL, 9IU/mL and 10 IU/mL), respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after the standard solution is added by taking 460nm as an excitation wavelength, and obtaining a fluorescence intensity change value of the carbon quantum dot solution at the maximum emission wavelength 542nm of the carbon quantum dot solution before and after the standard solution is added;
(3) Drawing a standard curve according to the change value of the fluorescence intensity obtained in the step (2) and the concentration of the corresponding oxytocin standard solution to obtain a linear equation;
wherein, the concentration of the oxytocin standard solution is in the range of 0.2-5IU/mL, the linear equation is y=21382x+12486, R 2 = 0.9943, where y is the variation of fluorescence intensity, x is the concentration of the oxytocin standard solution, and R is the linear fitting constant;
the linear equation of the concentration of the oxytocin standard solution in the range of 5-10IU/mL is y=5625x+91977, R 2 = 0.9937, where y is the variation of fluorescence intensity, x is the concentration of the oxytocin standard solution, and R is the linear fitting constant;
(4) And (3) adding 100 mu L of the to-be-detected oxytocin solution (with the known concentration of 6.67IU/mL and measuring for 6 times in parallel) into 50 mu L of the carbon quantum dot solution prepared in the step (1), respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after adding the to-be-detected oxytocin solution by taking 460nm as an excitation wavelength to obtain a fluorescence intensity change value of the carbon quantum dot solution at the maximum emission wavelength 542nm of the to-be-detected oxytocin solution relative to the carbon quantum dot solution before adding the to-be-detected oxytocin solution, and substituting the fluorescence intensity change value into a linear equation to obtain the concentration of the to-be-detected oxytocin solution of 6.91IU/mL.
Test examples
The characterization and performance of the carbon quantum dots with high fluorescence performance for detecting oxytocin prepared in examples 1-3 are basically consistent, and the following detection is performed by taking example 1 as an example.
1. The high-fluorescence carbon quantum dots for detecting oxytocin prepared in the example 1 are subjected to high-resolution transmission electron microscopy, particle size, infrared detection and ultraviolet detection, and the results are shown in figures 1-4.
As is clear from FIGS. 1 to 2, the synthesized carbon dots have a uniform particle size and an average particle size of 8.28nm.
As can be seen from FIG. 3, 3360cm -1 And 3031cm -1 Is the telescopic vibration peak of amino and hydroxyl, 1621-1460cm -1 Is the stretching vibration peak of benzene ring frameworkC＝C，1276cm -1 Is C-N substituted telescopic vibration peak on benzene ring of 745.64cm -1 The C-H stretching vibration peak on the ortho-disubstituted benzene ring shows that the synthesized carbon point has a benzene ring skeleton, the surface has hydroxyl and amino, and the existence of a large number of polar hydroxyl and amino leads the carbon point to have good water solubility.
As can be seen from FIG. 4, the carbon dot solution has maximum absorption at 280nm and 230nm, further indicating that the carbon dot has a conjugated double bond structure.
2. The high fluorescence carbon quantum dot solution for detecting oxytocin prepared in example 1 is subjected to fluorescence spectrum detection, and the result is shown in fig. 5. As can be seen from fig. 5, the maximum excitation wavelength of the present carbon quantum dot was 460nm, and the maximum emission wavelength was 542nm.
3. The high fluorescence carbon quantum dot solution for detecting oxytocin prepared in example 1 is continuously irradiated for 10 hours by using 460nm of excitation light, and the fluorescence signal is shown in fig. 6. As can be seen from fig. 6, the carbon dots prepared by the method have strong photo-bleaching resistance and good photo-stability.
4. Effect of interfering substances on the results of the oxytocin assay
Preparing 50 mu L of carbon quantum dot solution (1 mg/mL) of the carbon quantum dot for detecting the oxytocin, adding 100 mu L of oxytocin standard solution (the mass concentration is 1.67 ug/mL) with the concentration of 1IU/mL, respectively adding 50 mu L of water or aqueous solutions of different interfering substances (each group is measured in parallel for 6 times), and respectively measuring the fluorescence intensity of a reaction system at the maximum emission wavelength of 542nm after adding water and adding different interfering substances by taking 460nm as an excitation wavelength; the fluorescence intensity of the reaction system after the addition of the interfering substance was compared with that of the reaction system with water to obtain a relative error (%), and the results are shown in Table 1.
As shown in Table 1, common interfering ions such as common metal ions in water and various amino acids possibly introduced in the oxytocin extraction process and auxiliary material chlorobutanol in the oxytocin injection have no influence on the measurement result (the relative error is within +/-5 percent, and the influence of interfering substances on the measurement is negligible), so that the carbon quantum dot has good selectivity on the oxytocin, is not interfered by other substances during the measurement, and can be used for measuring the oxytocin.
TABLE 1 influence of interfering substances on measurement results (n=6)
5. Accuracy and precision
The recovery rate and the precision of the measurement results of examples 1 to 3 were measured, and the results are shown in Table 2. As can be seen from Table 2, the carbon quantum dots of the invention have good accuracy and precision in detecting the oxytocin content.
Table 2 determination of accuracy and precision of method (n=6)
6. Detection limit
Repeating the measurement of the fluorescence intensity of the carbon quantum dot solution before the standard solution is added in the step (2) of the example 1 for 11 times, calculating the standard deviation of the blank fluorescence intensity, obtaining the slope of the standard curve according to the standard curve in the step (3), and calculating the detection limit LOD with the following formula by using the signal to noise ratio S/n=3:
lod=3×standard deviation of blank fluorescence intensity/standard curve slope
The detection limit of the oxytocin is 0.0196IU/mL.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (6)
1. The application of the high-fluorescence-performance carbon quantum dot for detecting the oxytocin in the aspect of detecting the oxytocin content is characterized in that the preparation method of the high-fluorescence-performance carbon quantum dot for detecting the oxytocin comprises the following steps:
(1) Uniformly mixing a carbon source, a nitrogen source and deionized water, and then carrying out hydrothermal reaction to obtain a reaction solution;
(2) Cooling the reaction solution prepared in the step (1), and sequentially filtering, dialyzing and freeze-drying to prepare the carbon quantum dots with high fluorescence performance for detecting oxytocin;
in the step (1), the carbon source is quercetin;
in the step (1), the nitrogen source is o-phenylenediamine.
2. The application of the high-fluorescence carbon quantum dot for detecting the oxytocin according to claim 1, wherein in the step (1), the molar volume ratio of the carbon source to the nitrogen source to the deionized water is 0.5mmol:0.25-2mmol:10-25mL.
3. The use of the carbon quantum dot with high fluorescence performance for detecting the oxytocin according to claim 1, wherein in the step (1), the carbon quantum dot is subjected to hydrothermal reaction at 160-240 ℃ for 4-8 hours.
4. The method for detecting the content of the oxytocin is characterized by comprising the following steps of:
(1) Mixing the carbon quantum dot for detecting high fluorescence performance of oxytocin according to any one of claims 1-3 with water to obtain a carbon quantum dot solution;
(2) Respectively adding different concentrations of oxytocin standard solutions into the carbon quantum dot solution prepared in the step (1), and respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after the standard solution is added by taking 460nm as an excitation wavelength to obtain a fluorescence intensity change value of the carbon quantum dot solution after the standard solution is added relative to the carbon quantum dot solution before the standard solution is added at the maximum emission wavelength;
(3) Drawing a standard curve according to the change value of the fluorescence intensity obtained in the step (2) and the concentration of the corresponding oxytocin standard solution to obtain a linear equation;
(4) And (3) adding the to-be-detected oxytocin solution into the carbon quantum dot solution prepared in the step (1), respectively measuring fluorescence emission spectra of the carbon quantum dot solution before and after adding the to-be-detected oxytocin solution by taking 460nm as an excitation wavelength to obtain a fluorescence intensity change value of the carbon quantum dot solution after adding the to-be-detected oxytocin solution relative to the carbon quantum dot solution before adding the to-be-detected oxytocin solution at the maximum emission wavelength, and substituting the fluorescence intensity change value into a linear equation to obtain the concentration of the to-be-detected oxytocin solution.
5. The method for detecting oxytocin content according to claim 4, wherein in the step (1), the concentration of the carbon quantum dot solution is 0.05-1.5mg/mL.
6. The method for detecting the oxytocin content according to claim 4, wherein in the step (2), the volume ratio of the carbon quantum dot solution to the oxytocin standard solution is 1:0.5-2.
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