CN115497558A - 在基质上使用具有间隙的挂锁对来自组织的基因进行空间测序的方法 - Google Patents

在基质上使用具有间隙的挂锁对来自组织的基因进行空间测序的方法 Download PDF

Info

Publication number
CN115497558A
CN115497558A CN202210684664.9A CN202210684664A CN115497558A CN 115497558 A CN115497558 A CN 115497558A CN 202210684664 A CN202210684664 A CN 202210684664A CN 115497558 A CN115497558 A CN 115497558A
Authority
CN
China
Prior art keywords
sample
oligonucleotide
single stranded
tissue
padlock
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210684664.9A
Other languages
English (en)
Chinese (zh)
Inventor
H·迈耶
R·皮纳德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Meitianshi Biotechnology Co ltd
Original Assignee
Meitianshi Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meitianshi Biotechnology Co ltd filed Critical Meitianshi Biotechnology Co ltd
Publication of CN115497558A publication Critical patent/CN115497558A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/30Detection of binding sites or motifs
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/50Mutagenesis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN202210684664.9A 2021-06-18 2022-06-17 在基质上使用具有间隙的挂锁对来自组织的基因进行空间测序的方法 Pending CN115497558A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21180189 2021-06-18
EP21180189.9 2021-06-18

Publications (1)

Publication Number Publication Date
CN115497558A true CN115497558A (zh) 2022-12-20

Family

ID=76522852

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210684664.9A Pending CN115497558A (zh) 2021-06-18 2022-06-17 在基质上使用具有间隙的挂锁对来自组织的基因进行空间测序的方法

Country Status (4)

Country Link
US (1) US20220403462A1 (enExample)
EP (1) EP4105339B1 (enExample)
JP (1) JP2023001092A (enExample)
CN (1) CN115497558A (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6730525B2 (ja) 2016-11-21 2020-07-29 ナノストリング テクノロジーズ,インコーポレイティド 化学組成物とそれを利用する方法
JP7525402B2 (ja) 2018-05-14 2024-07-30 ナノストリング テクノロジーズ,インコーポレイティド 化学的組成物とそれを利用する方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
EP4589017A3 (en) * 2017-10-06 2025-10-08 10x Genomics, Inc. Rna templated ligation
EP3938538A1 (en) * 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing

Also Published As

Publication number Publication date
EP4105339B1 (en) 2023-05-24
US20220403462A1 (en) 2022-12-22
JP2023001092A (ja) 2023-01-04
EP4105339A1 (en) 2022-12-21

Similar Documents

Publication Publication Date Title
US20240416315A1 (en) Capturing oligonucleotides in spatial transcriptomics
US20230032082A1 (en) Spatial barcoding
JP7372927B6 (ja) 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法
CN111662960B (zh) 采用微流控装置的空间编码生物分析
CN105392898B (zh) 通过顺序杂交编条形码的分子多重标记
EP1711631B1 (en) Nucleic acid characterisation
JP6875998B2 (ja) 生物学的組織試料の分子プロファイルの空間マッピング
US10329614B2 (en) DNA sequencing and epigenome analysis
CN103228798B (zh) 使用固定的引物直接捕获、扩增及测序靶标dna
US9238834B2 (en) Efficient shotgun sequencing methods
US20160369329A1 (en) Multiplex labeling of molecules by sequential hybridization barcoding using probes with cleavable linkers
US20080274904A1 (en) Method of target enrichment
CN115497558A (zh) 在基质上使用具有间隙的挂锁对来自组织的基因进行空间测序的方法
Zhou et al. Encoding method of single-cell spatial transcriptomics sequencing
EP4108783A1 (en) Spatial sequencing with mictag
KR20180124789A (ko) 고순도 뉴클레오타이드 획득 방법 및 장치
US20240158784A1 (en) Molecular barcodes and related methods and systems
AU2024229387A1 (en) Compositions and methods for molecular barcoding
US20240287580A1 (en) Unit-dna composition for spatial barcoding and sequencing
Sokolowskei et al. Spatial transcriptomics in bone research: navigating hype and hurdles
JP2023175664A (ja) RT-PCRを用いてin situ標的増幅と空間固有分子識別子(SUMI)同定とを組み合わせた方法
EP4623079A1 (en) Chromatin profiling compositions and methods
CN116970689A (zh) 原位生成的rca产物的非原位测序
CN119452098A (zh) 使用适体的空间mRNA/蛋白质共测定

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination