CN115494050B - Low-light-level collection method, low-light-level collection device and luminescent bacteria low-light-level detection module - Google Patents
Low-light-level collection method, low-light-level collection device and luminescent bacteria low-light-level detection module Download PDFInfo
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Abstract
The invention discloses a low-light-level collection method, a low-light-level collection device and a luminescent bacteria low-light-level detection module, relates to the technical field of luminescent bacteria low-light-level detection, and solves the problem that the traditional luminescent bacteria detection method is less in light collection, and the technical scheme key points of the low-light-level collection method are as follows: includes receiving diverging light; performing first reflection on the divergent light to obtain collimated light; performing second reflection on the collimated light to obtain light converged at one point; by adopting the technical scheme, through twice reflection, divergent light at the light source is converted into collimated light, and the collimated light is further converged at one point, so that weak light is collected, and when the method is applied to detection of luminescent bacteria, the light collection rate of the luminescent bacteria can be improved, and the quantity of the luminescent bacteria reagent required by single detection is reduced.
Description
Technical Field
The invention relates to the technical field of low-light-level detection of luminescent bacteria, in particular to a low-light-level collection method, a low-light-level collection device and a low-light-level detection module of the luminescent bacteria.
Background
The luminescent bacteria emit blue-green light (about 450-490 nm) during metabolism, and if the metabolic process is affected, the luminous intensity is changed. Toxic substances contained in the water sample can inhibit normal metabolism of bacteria, so that the luminous intensity is reduced. The greater the toxicity, the stronger the inhibition effect and the smaller the luminescence intensity of the bacteria, and the intensity of water poisoning is evaluated by measuring the decrease of the luminescence intensity after the luminescent bacteria are contacted with a water sample relative to the intensity after the luminescent bacteria are contacted with a reference water sample (namely the relative luminescence inhibition rate).
ISO11348-3/GB-T15441 1995 utilizes the difference of luminescent strains on the water toxicity luminescence brightness to detect the water toxicity strength.
The water quality biotoxicity detector measures the acute biotoxicity caused by heavy metals and other organic pollutants in a detected environmental sample by measuring the change of the luminosity of luminous bacteria. Compared with the traditional method for detecting the biological toxicity of fish, fleas and other aquatic organisms, the luminous bacteria method is simple, convenient, quick, sensitive, strong in adaptability, good in repeatability, high in precision, low in cost and wide in application, and the biological toxicity of toxic compounds, waste water and wastes can be measured.
The luminous bacteria method is a direct collection detection method: the equipment developed by various manufacturers in the market at present directly uses a photoelectric detector to detect the luminous intensity of luminescent bacteria on the wall of a glass container or the wall of a test tube, and a receiving port of the photoelectric detector is directly aligned with the glass container, so that the quantity of light rays which can be received by a receiving window is small and is basically equal to the light rays of one surface of the glass container, which faces the photoelectric detector, the light rays emitted by most other surfaces of the glass container cannot be effectively received, the collection of the light rays is small, the detection result sensitivity of the photoelectric sensor is low, the upper limit value of the detection is low, the required quantity of the luminescent bacteria is large, the receiver has the defects that the receiver is close to a luminescent bacteria vessel, and the like.
Disclosure of Invention
The utility model aims at providing a shimmer collection method, shimmer collection device and luminous bacteria shimmer detect module, and the intention is utilizing a special shimmer collection method, device and brand-new luminous bacteria shimmer to detect the module, solves traditional luminous bacteria detection method light and collects few problem, collection light that can furthest to reach the purpose that improves the sensitivity that detects.
The first aspect of the present application provides a method for collecting dim light, which is implemented by the following technical solutions: comprises that
Receiving the diverging light;
performing first reflection on the divergent light to obtain collimated light;
and performing secondary reflection on the collimated light to obtain light converged at one point.
By adopting the technical scheme, through twice reflection, divergent light at the light source is converted into collimated light, and the collimated light is further converged at one point, so that weak light is collected, and when the method is applied to detection of luminescent bacteria, the light collection rate of the luminescent bacteria can be improved, and the quantity of the luminescent bacteria reagent required by single detection is reduced.
The second aspect of the present application provides a shimmer collection device for implementing the above-mentioned shimmer collection method: comprises that
The first reflecting surface, the second reflecting surface and the volume tube;
the first reflecting surface and the second reflecting surface are paraboloids of revolution, and the first reflecting surface is connected with the second reflecting surface to form a cavity;
the volume tube is arranged in the cavity and comprises a spherical cavity and a connecting rod which are mutually connected, and the connecting rod is arranged in the cavity formed by the first reflecting surface and the second reflecting surface in a penetrating mode and is used for fixing the spherical cavity in the cavity.
By adopting the technical scheme, the luminous bacteria culture solution is contained in the spherical cavity of the volume tube, the light source is uniformly emitted through the spherical cavity to form divergent light, the divergent light reaches the first reflecting surface and is reflected by the first reflecting surface to form collimated light, the collimated light reaches the second reflecting surface and is reflected by the second reflecting surface, so that the light of the electric light source is guaranteed to be converged at a focus as much as possible, and low-light collection is realized; when the fluorescent probe is applied to luminescent bacteria detection, weak light of the luminescent bacteria can be gathered, the use and collection rate of the luminescent bacteria is improved, and the use amount of the luminescent bacteria is reduced.
Furthermore, the focal point of the first reflecting surface, the focal point of the second reflecting surface and the center of the spherical cavity are located on the same straight line, and the incidence direction of the collimated light is parallel to the main axis of the second reflecting surface.
Further, the center of the spherical cavity is located at the focal point of the first reflecting surface, and the focal point of the second reflecting surface is located outside the spherical cavity.
Furthermore, the volume tube is made of transparent acrylic materials or glass.
Further, the inner wall surfaces of the first reflecting surface and the second reflecting surface are formed by vacuum chromium plating or gold polishing.
Further, the method also comprises the following steps: the transmitting cup and the collecting cup are movably connected, the inner wall surfaces of the transmitting cup and the collecting cup are sunken, the first reflecting surface and the second reflecting surface are respectively placed on the inner wall surfaces of the transmitting cup and the collecting cup, and fixing grooves corresponding to the connecting rods are further formed in the inner wall surfaces of the transmitting cup and the collecting cup.
Furthermore, a plug connector is arranged on the connecting rod, and a positioning hole matched with the plug connector is arranged in the fixing groove.
This application third aspect provides a luminous fungus shimmer detects module, obtains through following technical scheme: the glimmer collection device comprises the glimmer collection device and the photoelectric sensor, wherein a window is arranged on the surface of the second reflecting surface and connected with the photoelectric sensor, and a light receiving surface of the photoelectric sensor is located at the focus of the second reflecting surface or is located on one side, close to the spherical cavity, of the focus of the second reflecting surface.
By adopting the technical scheme, the luminous bacteria are contained in the spherical cavity to emit uniform divergent light, the divergent light is reflected by the first reflecting surface to form collimated light, the collimated light is reflected by the second reflecting surface to converge at the focus of the second reflecting surface, the light converged at the focus is transmitted to the photoelectric sensor through the light receiving surface, and an optical signal is converted into an electric signal for quantitative detection; on one hand, the light amplitude received by the photoelectric sensor can be greatly improved, and the detection sensitivity, the resolution and the upper limit value are improved; on the other hand, the dependence on the micro-light detection capability of the photoelectric sensor can be reduced, so that the type selection cost is reduced, the change of luminescent bacteria is more obvious after the light source is converged, and the change of water quality can be measured by adopting a common photoelectric sensor.
Further, the volume of the spherical cavity is 1-4ml.
Compared with the prior art, the method has the following beneficial effects: the utility model relates to a low light collecting method, a device and a luminescent bacteria low light detecting device; firstly, light captured by the sensor is improved by N times, detection precision is improved, and the detection upper limit value of the sensor is improved; secondly, the requirements on the sensor can be reduced, the cost of the sensor is reduced, the light source is sufficiently gathered, a high-precision photon counter is not needed, and the problem of alignment of the photon counter equipment can be avoided; thirdly, the using amount of the luminescent bacteria during detection is reduced, and the detection cost is saved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:
FIG. 1 is a schematic diagram of the detection of conventional luminescent bacteria according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of another conventional luminescent bacterium detection provided by an embodiment of the present invention;
FIG. 3 is a schematic flow chart of a method for collecting micro light according to an embodiment of the present invention;
FIG. 4 is a schematic diagram illustrating a method for collecting micro light according to an embodiment of the present invention;
FIG. 5 is a schematic view of a micro light collection device according to an embodiment of the present invention;
FIG. 6 is a schematic diagram illustrating light collection according to an embodiment of the present invention;
fig. 7 is a schematic structural diagram of a luminescent bacteria micro-light detection module according to an embodiment of the present invention;
FIG. 8 is a diagram illustrating the detection result of a conventional micro-light detection device for luminescent bacteria according to an embodiment of the present invention;
fig. 9 is a detection result of the luminescent bacteria glimmer detection apparatus according to the present application provided in an embodiment of the present invention.
Reference numbers and corresponding part names in the drawings:
1. a containment tube; 11. a spherical cavity; 12. a connecting rod; 2. a first reflective surface; 3. a second reflective surface; 4. a photoelectric sensor.
Detailed Description
Hereinafter, the term "includes" or "may include" used in various embodiments of the present application indicates the presence of the applied function, operation or element, and does not limit the addition of one or more functions, operations or elements. Furthermore, as used in various embodiments of the present application, the terms "comprising," "having," and their derivatives, are intended to be inclusive and mean only that a particular feature, number, step, operation, element, component, or combination of the foregoing is meant, and should not be construed as first excluding the presence of, or adding to, one or more other features, numbers, steps, operations, elements, components, or combinations of the foregoing.
In various embodiments of the present application, the expression "or" at least one of B or/and C "includes any or all combinations of the words listed simultaneously. For example, the expression "B or C" or "at least one of B or/and C" may include B, may include C, or may include both B and C.
Expressions (such as "first", "second", and the like) used in various embodiments of the present application may modify various constituent elements in various embodiments, but may not limit the respective constituent elements. For example, the above description does not limit the order and/or importance of the elements described. The foregoing description is for the purpose of distinguishing one element from another. For example, the first user device and the second user device indicate different user devices, although both are user devices. For example, a first element could be termed a second element, and, similarly, a second element could be termed a first element, without departing from the scope of various embodiments of the present application.
It should be noted that: if it is described that one constituent element is "connected" to or "connected" with another constituent element, the first constituent element may be directly connected to the second constituent element, and the third constituent element may be "connected" between the first constituent element and the second constituent element. In contrast, when one constituent element is "directly connected" to or with another constituent element, it is understood that there is no third constituent element between the first constituent element and the second constituent element.
The terminology used in the various embodiments of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the various embodiments of the present application. As used herein, the singular forms are intended to include the plural forms as well, unless the context clearly indicates otherwise. Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the various embodiments of the present application belong. The terms (such as those defined in commonly used dictionaries) should be interpreted as having a meaning that is consistent with their contextual meaning in the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein in various embodiments.
To make the purpose, technical solution and advantages of the present application more apparent, the present application is further described in detail below with reference to examples and drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present application and are not used as limitations of the present application.
The traditional detection method of luminous bacteria is a direct collection detection method, which is shown in fig. 1-2 and comprises the following steps: the device comprises a glass vessel for containing luminescent bacteria and a photoelectric sensor 4, wherein the photoelectric sensor 4 is arranged on one side of the glass vessel to detect the side light of the glass vessel, and the photoelectric sensor 4 is arranged at the bottom of the glass vessel to detect the slave light at the bottom of the glass vessel.
No matter the side light or the bottom light is detected, the receiving window of the photoelectric sensor 4 is directly aligned to the glass container, the photoelectric sensor 4 can only receive the light of the glass container on the opposite surface of the receiving window, and most of the light emitted by other surfaces of the glass container cannot be effectively received; the luminous intensity of the luminous bacteria is low, the luminous bacteria is glimmer, the visibility of naked eyes is low, and when the luminous bacteria is directly detected, the required dosage of the luminous bacteria is large, otherwise, the amplitude of light received by the photoelectric sensor 4 is low, the upper limit value of detection is low, and the sensitivity is not high; and during detection, the intensity of light intensity is in direct proportion to one square of the receiving distance, so that the receiving window is required to be very close to the luminescent bacteria vessel, and the arrangement of a detection assembly is limited.
In view of the problem that above-mentioned direct detection exists, this application first aspect provides a shimmer collection method, holds the many curved surfaces of household utensils reflection light through luminous fungus and collects for luminous fungus's light collection rate promotes by a wide margin, can reduce the required luminous fungus reagent volume of single detection by a wide margin.
Referring to fig. 3, fig. 3 is a schematic flow chart of a method for collecting dim light, comprising:
receiving the diverging light;
performing first reflection on the divergent light to obtain collimated light;
and performing secondary reflection on the collimated light to obtain light converged at one point.
Specifically, as described with reference to fig. 4, the light source is a point light source, emits uniform divergent light, the divergent light reaches the first reflecting surface 2, is reflected by the first reflecting surface 2 to form parallel collimated light, the collimated light reaches the second reflecting surface 3, is reflected by the second reflecting surface 3, and converges at a point, which is a focal point of the second reflecting surface 3.
Adopt the shimmer collection method that this application provided, through twice reflection, convert the divergent light of light source department into collimated light, further assemble in a bit again, realize the collection to the weak light, when being applied to the luminous fungus and examining, can promote the light collection rate of luminous fungus, reduce the required luminous fungus reagent volume of single detection.
The utility model provides a shimmer detection device for realize foretell shimmer detection method, the light that sends luminous fungus through the optics curved surface that changes glassware shape and external special design is directly and indirectly collected and is sampled, reaches the purpose of collecting more light.
Referring to fig. 5, fig. 5 is a schematic structural diagram of a micro light collecting device, including:
a first reflecting surface 2, a second reflecting surface 3 and a volume pipe 1;
the first reflecting surface 2 and the second reflecting surface 3 are paraboloids of revolution, and the first reflecting surface 2 is connected with the second reflecting surface 3 to form a cavity;
the volume tube 1 is arranged in the cavity, the volume tube 1 comprises a spherical cavity 11 and a connecting rod 12 which are connected with each other, and the connecting rod 12 penetrates through the cavity formed by the first reflecting surface 2 and the second reflecting surface 3 and is used for fixing the spherical cavity 11 in the cavity.
Specifically, the interior of the spherical cavity 11 of the volume tube 1 is filled with the photobacteria culture solution, the center of the spherical cavity 11 is a light source center, the light source is uniformly emitted through the spherical cavity 11 to form divergent light, the divergent light reaches the first reflecting surface 2 and is reflected by the first reflecting surface 2 to form collimated light, and the collimated light reaches the second reflecting surface 3 and is reflected by the second reflecting surface 3 to be converged at one point.
In a possible embodiment, the focal point of the first reflecting surface 2, the focal point of the second reflecting surface 3 and the center of the spherical cavity 11 are located on the same straight line, and the incidence direction of the collimated light is parallel to the main axis of the second reflecting surface 3.
In a possible embodiment, the center of the spherical cavity 11 is located at the focal point of the first reflecting surface 2, and the focal point of the second reflecting surface 3 is located outside the spherical cavity 11. The light reflected by the first reflecting surface 2 and the second reflecting surface 3 is ensured to be converged outside the spherical cavity 11, namely, converged at the focus of the second reflecting surface 3. Referring to fig. 6, the center of the spherical cavity 11 is located at the focal point of the first reflecting surface 2, and the electric light source of the spherical cavity 11 is reflected by the first reflecting surface 2 and the second reflecting surface 3 and converges at the focal point of the second reflecting surface 3.
In one possible embodiment, the volume tube 1 is made of transparent acrylic material or glass, and the inner wall surfaces of the first reflecting surface 2 and the second reflecting surface 3 are formed by vacuum plating chrome or gold polishing.
It should be noted that the above-mentioned materials of the containment tube 1 and the manufacturing process of the first and second reflection surfaces 2 and 3 are only some preferred embodiments, and do not constitute a limitation to the present application, and all the materials that make the containment tube 1 transparent and the manufacturing process that makes the first and second reflection surfaces 2 and 3 smooth surfaces with high reflectivity are known to those skilled in the art and fall within the protection scope of the present application.
In a possible embodiment, the low light collection device further comprises: the internal face of swing joint's transmission cup and collection cup, transmission cup and collection cup is sunken, places first plane of reflection 2 and second plane of reflection 3 respectively, and the internal face of transmission cup and collection cup still is provided with the fixed slot that corresponds with connecting rod 12. Specifically, the transmission cup is the arc cup with collect the cup, can adopt ABS plastics to make, when the transmission cup is connected with the collection cup, first plane of reflection 2 and the 3 looks counterbalance vacuoles of second plane of reflection in it, the internal face of transmission cup and collection cup all is provided with the tubulose fixed slot of caving in formation, during the assembly, the fixed slot can be arranged in to the connecting rod 12 of volume pipe 1, the assembly back, and the cavity is airtight, avoids external light to disturb and inside light to reveal.
Furthermore, a plug connector is arranged on the connecting rod 12, and a positioning hole matched with the plug connector is arranged in the fixing groove. Specifically, the plug connector is columnar, and during assembly, the plug connector on the connecting rod 12 can be inserted into the positioning hole to ensure that the center of the spherical cavity 11 is located at the focus of the first reflecting surface and located on the same straight line with the focus of the second reflecting surface.
By adopting the glimmer collection device, the light of the point light source is directly or indirectly converged at the focus of the second reflecting surface 3 through the first reflecting surface 2 and the second reflecting surface 3 which are specially arranged, the volume tube 1 is used for containing the light source and is fixed at the appointed position of the cavity through the connecting rod 12, the whole cavity is closed, the light of the electric light source is guaranteed to be converged at the focus as much as possible, and glimmer collection is realized; when the fluorescent probe is applied to luminescent bacteria detection, weak light of the luminescent bacteria can be gathered, the use and collection rate of the luminescent bacteria is improved, and the use amount of the luminescent bacteria is reduced.
The utility model provides a light-emitting bacterium shimmer detects module is applied to light-emitting bacterium shimmer with foretell shimmer collection device and detects during to detect quality of water.
Referring to fig. 7, fig. 7 is a schematic structural diagram of a luminescent bacteria glimmer detection module, a photoelectric sensor 4 is added on the basis of the glimmer collection device, a window is arranged on the surface of the second reflection surface 3 and connected with the photoelectric sensor 4, a light receiving surface of the photoelectric sensor 4 is located at a focus of the second reflection surface 3, or a focus of the second reflection surface 3 is close to the spherical cavity 11, namely, one side of the volume tube 1.
In an ideal state, light rays of the electric light source of the spherical cavity 11 are converged at the focus of the second reflecting surface 3 through the first reflecting surface 2 and the second reflecting surface 3, and the light ray receiving surface is arranged at the focus of the second reflecting surface 3, so that the maximum light rays can be received; however, considering the loss of light propagating in the medium and the area of the light receiving surface, the light receiving surface may be moved a certain distance toward the spherical cavity 11, so that the converged light enters the light receiving surface in a careful manner, and the optical path length is shortened to reduce the loss of light propagating in the medium. Through the simulation result of EDA optical simulation software, the light receiving surface can be moved 1mm from the focus of the second reflecting surface 3 to the spherical cavity 11, and the light flux value received by the light receiving surface is maximum at the moment.
It should be noted that, in actual use, the specific position of the light receiving surface may be comprehensively adjusted according to the material of the first reflecting surface 2, the material of the spherical cavity 11, the material of the second reflecting surface 3, the cavity medium, and the area of the light receiving surface, so that the light intensity received by the light receiving surface is the maximum.
It should be noted that the light receiving surface, i.e. the plane on which the photoelectric sensor 4 senses light, is transformed into an electrical parameter by the light receiving surface.
Specifically, sufficient photogenic bacteria culture solution or a to-be-detected aqueous solution doped with photogenic bacteria is poured into the spherical cavity 11 of the volume tube 1, the spherical cavity 11 uniformly emits divergent light, the divergent light is reflected by the collecting cup to form collimated light, the collimated light is emitted by the collecting cup and converged to the light receiving surface, the collimated light is transmitted to the photoelectric sensor 4 through the light receiving surface, and an optical signal is converted into an electrical signal to be quantitatively detected.
In a possible embodiment, the volume of the spherical cavity 11 is set to 1-4ml according to the actual amount of the detection reagent for water quality monitoring, and specifically, if the volume of the spherical cavity 11 is set to 2ml, the corresponding actual spherical cavity 11 has an inner diameter of 13.6mm, a thickness of 1mm and an outer diameter of 15.6mm.
The volume of the spherical cavity 11 is only a preferred embodiment, and is not limited to the device itself, and the volume of the spherical cavity 11 may be set by itself according to experimental requirements in actual use.
By adopting the luminescent bacteria glimmer detection module, on one hand, the light amplitude value received by the photoelectric sensor 4 can be greatly improved, and the detection sensitivity, the resolution and the upper limit value are improved; on the other hand, the cost of the photoelectric sensor 4 can be reduced, the change of luminescent bacteria is more obvious after the light source is gathered, the water quality change can be measured by adopting the common photoelectric sensor 4, and accurate measurement can be realized without carrying high-precision operational amplifier or matching strict anti-interference capability.
In order to further explain the effect that the luminous fungus shimmer detection device that this application provided, this application fourth aspect provides CAE emulation test result, compares this application luminous fungus shimmer detection device and traditional luminous fungus shimmer detection device.
Adopt 250 ten thousand light simulation, simulate traditional direct acquisition detection method respectively and detect, and the luminous bacteria shimmer of this application detects the module.
Referring to the detection results shown in fig. 8-9, fig. 8 is a detection result of a conventional luminescent bacteria glimmer detection device, and fig. 9 is a detection result of the luminescent bacteria glimmer detection device of the present application; compare than traditional testing result, this application testing result photon number has promoted more than 10 times, if when not diluting the tradition method detect out the photon and be 200 ten thousand units, this application device detects the photon and is 2300 ten thousand units, and the check-out period is 1000 ms 1 times, and the number of times of detection is single 60 times to get the average value.
In conclusion, the low light collecting method, the device and the luminescent bacteria low light detection device are adopted; firstly, light captured by the sensor is improved by N times, detection precision is improved, and the detection upper limit value of the sensor is improved; secondly, the requirements on the sensor can be reduced, the cost of the sensor is reduced, the light source is sufficiently converged, a high-precision photon counter is not needed, and the problem of equipment alignment of the photon counter can be avoided; thirdly, the using amount of the luminescent bacteria during detection is reduced, and the detection cost is saved.
The above embodiments are provided to further explain the objects, technical solutions and advantages of the present invention in detail, it should be understood that the above embodiments are merely exemplary embodiments of the present invention and are not intended to limit the scope of the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. The utility model provides a luminous fungus shimmer detects module which characterized by: comprises a low light collecting device and a photoelectric sensor;
the micro light collecting device includes: the first reflecting surface, the second reflecting surface and the volume tube; the first reflecting surface and the second reflecting surface are paraboloids of revolution, and the first reflecting surface is connected with the second reflecting surface to form a closed cavity; the volume tube is arranged in the cavity and comprises a spherical cavity and a connecting rod which are mutually connected, and the connecting rod is arranged in the cavity formed by the first reflecting surface and the second reflecting surface in a penetrating way and is used for fixing the spherical cavity in the cavity;
the spherical cavity part extends out of the opening of the first reflecting surface;
the surface of the second reflecting surface is provided with a window, the window is connected with the photoelectric sensor, and a light receiving surface of the photoelectric sensor is positioned on one side, close to the spherical cavity, of a focus of the second reflecting surface and used for ensuring that the sum of light rays from all directions reaching the light receiving surface is the maximum, and the light intensity superposition total value of the light receiving surface reaches the maximum;
the shimmer collection device is used for realizing a shimmer collection method and comprises the following steps:
receiving the diverging light;
performing first reflection on the divergent light to obtain collimated light;
and performing secondary reflection on the collimated light to obtain light converged at one point.
2. The module of claim 1, wherein: the focus of the first reflecting surface, the focus of the second reflecting surface and the center of the spherical cavity are located on the same straight line, and the incidence direction of collimated light is parallel to the main shaft of the second reflecting surface.
3. The module of claim 2, wherein: the center of the spherical cavity is located at the focus of the first reflecting surface, and the focus of the second reflecting surface is located outside the spherical cavity.
4. The module of claim 1 for testing micro-light of luminescent bacteria, wherein: the volume tube is made of transparent acrylic material or glass.
5. The module of claim 1 for testing micro-light of luminescent bacteria, wherein: the inner wall surfaces of the first reflecting surface and the second reflecting surface are formed by adopting vacuum chromium plating or gold polishing.
6. The module of claim 1 for testing micro-light of luminescent bacteria, wherein: further comprising: the transmitting cup and the collecting cup are movably connected, the inner wall surfaces of the transmitting cup and the collecting cup are sunken, the first reflecting surface and the second reflecting surface are respectively placed on the inner wall surfaces of the transmitting cup and the collecting cup, and fixing grooves corresponding to the connecting rods are further formed in the inner wall surfaces of the transmitting cup and the collecting cup.
7. The module of claim 6, wherein: the connecting rod is provided with a plug connector, and the fixed groove is internally provided with a positioning hole matched with the plug connector.
8. The module of claim 1, wherein: the volume of the spherical cavity is 1-4ml.
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