CN115491363A - Preparation method and application of mesoporous nano material with antibacterial function - Google Patents
Preparation method and application of mesoporous nano material with antibacterial function Download PDFInfo
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- CN115491363A CN115491363A CN202211122054.6A CN202211122054A CN115491363A CN 115491363 A CN115491363 A CN 115491363A CN 202211122054 A CN202211122054 A CN 202211122054A CN 115491363 A CN115491363 A CN 115491363A
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- glucose oxidase
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- polyphenol
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- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 claims description 4
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- A61K38/00—Medicinal preparations containing peptides
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- A61K38/44—Oxidoreductases (1)
- A61K38/443—Oxidoreductases (1) acting on CH-OH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
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Abstract
The invention discloses a preparation method and application of a mesoporous nano material with an antibacterial function, and relates to the technical field of antibacterial materials. Mixing glucose oxidase, a metal ion source and a template for reaction, and then adding a polyphenol source for assembly to obtain the mesoporous nano material with the antibacterial function. The mesoporous nano material with the antibacterial function consists of glucose oxidase @ metal-polyphenol; the structure is as follows: the metal-polyphenol forms a network encapsulating the glucose oxidase. The glucose oxidase @ metal-polyphenol mesoporous nanomaterial prepared by the invention is used as a mesoporous material, and can quickly generate a cascade reaction under the activation of glucose, so that the enzyme activity is improved. As an antibacterial material, the antibacterial material has broad-spectrum bactericidal property, has a bactericidal effect within a certain time, is superior to free glucose oxidase and glucose oxidase with a non-mesoporous structure @ metal-polyphenol, and has an average sterilization rate of more than 99%.
Description
Technical Field
The invention relates to the technical field of antibacterial materials, in particular to a preparation method and application of a mesoporous nano material with an antibacterial function.
Background
Diseases caused by bacteria have become one of the biggest global health problems, and affect tens of thousands of people every year. Traditional antibacterial drugs are mainly antibiotics. In addition, some inorganic agents of metal inorganic salts may be used as the antibacterial material. However, these antimicrobial agents often have certain drawbacks. For this reason, new materials are being sought for antibacterial therapy. The natural enzyme is mainly extracted from microbial cells, and has the advantages of high catalytic activity, substrate specificity, catalytic diversity, mild reaction, adjustable activity and the like. Has attracted wide attention in the fields of environmental protection, disease diagnosis and treatment, antibiosis, etc. But the practical application of the method is greatly limited because of the defects of high preparation and purification cost, poor stability, easy deformation and the like. To solve these problems, new natural enzyme substitutes have been increasingly sought.
The nano enzyme is a natural enzyme substitute with great potential, and compared with natural enzyme, the nano enzyme has the advantages of low cost, high stability, easiness in batch production and storage and the like. The mimic peroxidase is an important class of nano-enzymes, and has wide application prospects. Has already achieved good research results in a plurality of fields such as clinical medicine, food safety, chemical monitoring and chemical production, etc. Polypyrrole nanoparticles, gold (Au) nanoparticles, and ferroferric oxide (Fe) 3 O 4 ) Nanoparticles, carbon nanotubes, and the like having excellent peroxidase activityAnd (3) catalytic activity. In the nano-enzyme, a considerable part of mechanisms relate to the generation of highly toxic hydroxyl radicals, so that the nano-enzyme also brings wide application of the material, such as antibiosis, anticancer and the like. However, the sterilization rate and the sterilization spectrum of the nano-enzyme in the prior art are still to be further improved.
Disclosure of Invention
Based on the content, the invention provides a preparation method and application of a mesoporous nano material with an antibacterial function.
In order to achieve the purpose, the invention provides the following scheme:
in one technical scheme of the invention, the mesoporous nano material with the antibacterial function comprises glucose oxidase @ metal-polyphenol, and has the structure as follows: the metal-polyphenol forms a network encapsulating the glucose oxidase.
Further, the metal in the glucose oxidase @ metal-polyphenol is Fe 3+ (ii) a The polyphenol in the glucose oxidase @ metal-polyphenol is gallic acid.
In the second technical scheme of the invention, the preparation method of the mesoporous nano material with the antibacterial function comprises the following steps:
mixing glucose oxidase, a metal ion source and a template for reaction, and then adding a polyphenol source for assembly to obtain the mesoporous nano material with the antibacterial function.
Further, the concentration of the glucose oxidase in the reaction system is 10-200 mug/mL, the concentration of the template is 0.1-0.5mM, the concentration of the metal ion source is 0.05-0.2mM, and the concentration of the polyphenol source is 0.1-1.0mM.
The appropriate concentration ratio helps to obtain a metal-polyphenol nanomaterial with high catalase-like activity, above or below which the catalase-like activity of the metal-polyphenol nanomaterial is affected.
Further, the mixing reaction time is 15-120min; the assembling time is 2-12h;
the appropriate reaction time and assembly time are closely related to the metal-polyphenol nano material with higher catalase-like activity. And after the assembly is finished, the method also comprises a step of removing the template and unreacted raw materials through ultrafiltration or dialysis.
Further, the metal ion source is FeCl 3 ·6H 2 O; the template is a surfactant; the polyphenol source is one of gallic acid, ellagic acid, tannic acid, epigallocatechin gallate and caffeic acid. Further preferably, the polyphenol source is gallic acid.
Further, the surfactant is sodium deoxycholate.
The high biocompatibility of metal-NaDC can protect biomolecules from extreme non-physiological environments. The NaDC is used as a soft template, and the template can be removed by simple concentration difference to obtain a required structure, and the activity of the material is not influenced in subsequent application.
In the third technical scheme of the invention, the mesoporous nano material with the antibacterial function is applied to preparation of antibacterial materials.
In the fourth technical scheme of the invention, the mesoporous nano material with the antibacterial function is applied to killing bacteria.
The technical idea of the invention is as follows:
sodium deoxycholate (NaDC) is a water-soluble bile salt, and is of great importance in biology and medicine as a biocompatible surfactant for DNA and protein purification. The NaDC hydrogel can retain its highly active biomolecules. The metal-NaDC hydrogel is also a suitable template for synthesizing mesoporous materials because the size and the shape of the metal-NaDC hydrogel can be adjusted. The invention designs a mesoporous nano material with an antibacterial function, which mainly takes NaDC as a soft template, utilizes metal-polyphenol to form a network to wrap glucose oxidase, and forms the mesoporous material after physical washing. The material can generate hydroxyl free radicals to kill bacteria under the activation of glucose, thereby achieving the antibacterial effect.
The invention discloses the following technical effects:
the method is suitable for preparing various glucose oxidase @ metal-polyphenol mesoporous nanoparticles, and has the following advantages: 1) The raw materials are simple and easy to obtain; 2) The biocompatibility and the stability are good; 3) The synthesis steps are simple, and the mass production is easy.
The glucose oxidase @ metal-polyphenol mesoporous nanoparticles prepared by the invention are used as mesoporous materials, and can quickly generate cascade reaction under the activation of glucose, so that the enzyme activity is improved. As an antibacterial material, the metal-polyphenol composite antibacterial material has broad-spectrum bactericidal property, has a bactericidal effect within a certain time, is superior to free glucose oxidase and glucose oxidase @ metal-polyphenol with a non-mesoporous structure, and has an average sterilization rate of more than 99%.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic diagram of the formation of a glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in example 1 of the present invention;
FIG. 2 is a UV absorption spectrum of glucose oxidase @ ferric ion-gallic acid mesoporous nanomaterial (GOx @ Fe-GA) prepared in example 1 of the present invention, metal-polyphenol mesoporous nanoparticles (Fe-GA) prepared in example 2, and glucose oxidase (GOx);
FIG. 3 is a transmission scanning electron micrograph of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in example 1 of the present invention, wherein the left image is a transmission electron micrograph, and the right image is an EDS (electron-dispersive spectroscopy) energy spectrum data analysis image of the transmission electron micrograph;
FIG. 4 is a nitrogen isothermal adsorption curve and a pore size distribution diagram of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in example 1 of the present invention; wherein the left graph is a nitrogen isothermal adsorption curve, and the right graph is a pore size distribution graph;
FIG. 5 is a peroxidase-like enzyme activity test chart of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in example 1 of the present invention; wherein the left graph shows the change of the light absorption value of the glucose oxidase @ iron ion-gallic acid mesoporous nano material at 652nm when the glucose solution is used as a substrate, and the right graph shows the steady-state kinetic analysis of the glucose oxidase @ iron ion-gallic acid mesoporous nano material when the glucose solution is used as a substrate;
FIG. 6 is a test chart of the cascade reaction activity of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in example 1 of the present invention, wherein a represents H with different concentrations 2 O 2 When the solution is taken as a substrate, the light absorption value of the glucose oxidase @ iron ion-gallic acid mesoporous nano material at 652nm changes, and b represents H with different concentrations 2 O 2 When the solution is a substrate, analyzing steady-state kinetics of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial, wherein c represents that when TMA with different concentrations is the substrate, the absorbance value of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial at 652nm changes, and d represents that when TMA with different concentrations is the substrate, analyzing steady-state kinetics of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial;
fig. 7 shows the killing effect of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in example 1 of the present invention on staphylococcus aureus, a representative bacterium of gram-negative bacteria klebsiella pneumoniae and a positive bacterium with multiple drug resistance; wherein a represents gram-negative bacteria with multiple drug resistance, klebsiella pneumoniae, and b represents staphylococcus aureus which is a representative bacterium of positive bacteria;
fig. 8 shows the change of absorbance at 652nm of the fe ion-polyphenol mesoporous nanomaterial prepared in example 2 and example 4 when glucose solution is used as a substrate.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in the present disclosure, it is understood that each intervening value, to the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The sodium deoxycholate solution in the embodiment of the invention is specifically as follows: obtained by dissolving sodium deoxycholate in phosphate buffer solution (pH 7.4).
Example 1
Step 2, 5mL of 4.52mg/mL aqueous solution of Gallic Acid (GA) was slowly dropped into the above mixed solution and stirring was continued for 2 hours to obtain a pale purple liquid.
And 3, dialyzing the light purple liquid for 2 hours, and removing sodium deoxycholate, unreacted iron ions, gallic acid and glucose oxidase to obtain a solution containing glucose oxidase @ metal-polyphenol mesoporous nano material (glucose oxidase @ iron ions-gallic acid mesoporous nano material, abbreviated as GOx @ Fe-GA).
Fig. 1 is a schematic diagram of formation of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in the present example.
Fig. 3 is a transmission scanning electron micrograph of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in this example, wherein the left figure is a transmission electron micrograph, and the right figure is an EDS (electron spectroscopy) energy spectrum data analysis figure of the transmission electron micrograph. The test procedure was as follows: diluting the glucose oxidase @ iron ion-gallic acid mesoporous nano material by 10 times, dripping the diluted material into a molybdenum net, standing and naturally air-drying to obtain a TEM test sample. The material is spherical nano material with the grain diameter of about 100nm as can be seen from the left graph in the figure, the P element can be clearly seen from the right graph in the figure, and the fact that the material contains glucose oxidase can be confirmed. The GOx @ Fe-GA nanomaterial prepared in this example was a nanomaterial formed by aggregation of many small particles, and it was also explained in the nitrogen adsorption diagram that the pores of the nanomaterial were slit-shaped pores, which were formed due to the aggregation of particles, and thus the nanomaterial was in a large particle state as seen in the TEM diagram.
Fig. 4 is a nitrogen isothermal adsorption curve and a pore size distribution diagram of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in the present example; wherein, the left graph is a nitrogen isothermal adsorption curve, and the right graph is a pore diameter distribution graph. The test procedure was as follows: the nitrogen adsorption isotherm (Micromeritics, 3flex, usa) was measured at 77K and the pore size distribution of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial was determined using an automated gas adsorption analyzer. As can be seen from the left panel of the figure, the sample exhibits a standard type IV isotherm indicating the presence of mesoporesAnd (5) structure. At the same time, the isotherm shows a clear H3-type hysteresis loop. This indicates that the formation of slit-like pores is likely to result from particle aggregation, as shown in the TEM image of figure 3. From the right-hand side of the figure it can be seen that the main aperture width is
FIG. 5 is a peroxidase-like enzyme activity test chart of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in the present example; wherein the left graph is the change of the light absorption value at 652nm when the glucose solution is taken as a substrate, and the right graph is the steady-state kinetic analysis of the GOx @ Fe-GA nano material when the glucose solution is taken as a substrate. The test procedure was as follows: glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in this example (100 μ L,0.1 mmoL) was mixed with 3,3',5,5' -tetramethylbenzidine (300 μ L,20.0 mmoL), acetic acid buffer solution (2,300 μ L, pH = 4.5), and glucose solutions of different concentrations (300 μ L, 25.0-1.60 mg/mL). It was then tested for uv-visible absorption in the range of 400-800 nm. As can be seen from the left graph in the figure, the catalytic activity increases as the concentration of the glucose solution increases. Therefore, the glucose oxidase @ iron ion-gallic acid mesoporous nano material has peroxidase-like activity. From the right graph in the figure, it can be seen that Vm is 0.16 × 10 -8 M/s and Km were 0.24mM.
FIG. 6 is a test chart of the cascade reaction activity of the glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in this example, wherein a represents H with different concentrations 2 O 2 When the solution is taken as a substrate, the light absorption value of the glucose oxidase @ iron ion-gallic acid mesoporous nano material at 652nm changes, and b represents H with different concentrations 2 O 2 When the solution is used as a substrate, the steady-state kinetic analysis of the glucose oxidase @ iron ion-gallic acid mesoporous nano material is carried out, wherein c represents the change of the light absorption value of the glucose oxidase @ iron ion-gallic acid mesoporous nano material at 652nm (the two highest lines are overlapped) when TMA with different concentrations are used as the substrate, d represents the steady-state kinetic analysis of the glucose oxidase @ iron ion-gallic acid mesoporous nano material when TMA with different concentrations are used as the substrateAnd (6) analyzing. The test procedure was as follows: glucose oxidase @ ferric ion-gallic acid mesoporous nano material (100 muL, 0.1 mmoL) prepared in the example, 3,3',5,5' -tetramethylbenzidine (300 muL, 20.0-1.30 mM), acetic acid buffer solution (2300 muL, pH = 4.5) and H with different concentrations 2 O 2 (300. Mu.L, 25.0 to 1.60 mM) and then tested for UV-visible absorption in the 400-800nm range. As can be seen in FIG. 6, with 3,3',5,5' -tetramethylbenzidine and H 2 O 2 The concentration of the solution increases and the absorbance increases. Therefore, the glucose oxidase @ iron ion-gallic acid mesoporous nano material has the characteristic of cascade catalytic reaction.
The glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial prepared in this example was tested for its killing effect on gram-negative bacteria having multiple drug resistance, klebsiella pneumoniae (a) and representative bacteria, staphylococcus aureus (b), with positive bacteria, and the results are shown in fig. 7: the Klebsiella pneumoniae and Staphylococcus aureus solutions were separated and resuspended in sterile PBS at a concentration of 1 × 10 7 CFU/mL. Then 300. Mu.L of the bacterial solution and 300. Mu.L of the material solution (equivalent to the concentration of glucose oxidase (GOx), 300. Mu.g/mL) were placed in a 1.5mL centrifuge tube and put into a 37 ℃ bacterial incubator for static culture for 3 hours. After the completion of the culture, 100. Mu.L of the bacterial suspension was aspirated, dispersed in 900. Mu.L of sterile PBS, and then serially diluted, and the number of viable bacteria in the solution was measured by the plate coating method. Data were repeated three times per group and averaged.
The glucose oxidase @ iron ion-gallic acid prepared in this example had Minimal Inhibitory Concentration (MIC) and minimal inhibitory concentration (MBC) in multi-drug resistant ESKAPE pathogenic bacteria: separating multi-drug resistant ESKAPE pathogenic bacteria solution, and suspending in sterile PBS at concentration of 2 × 10 5 CFU/mL. Then 100. Mu.L of the bacterial solution and 100. Mu.L of the material solution (0 to 400. Mu.g/mL) were placed in a 96-well plate, and placed in a 37 ℃ bacterial incubator for static culture for 24 hours. MIC values are considered as the Minimum Inhibitory Concentration (MIC) values with no bacterial growth. Subsequently, the minimum inhibitory concentration (MBC) value was determined by a suspension of the minimum concentration that did not produce visible bacteria on the agar plate. Data were repeated three times per group and averaged. The results are shown in Table 1.
TABLE 1
As can be seen from table 1, the glucose oxidase @ iron ion-gallic acid mesoporous nano material particles prepared in example 1 of the present invention undergo a cascade reaction under activation of glucose (the culture medium contains glucose), generate enough hydroxyl radicals to kill bacteria, have a killing effect on multi-drug-resistant ESKAPE pathogenic bacteria, and have a broad-spectrum sterilization effect.
Example 2
Step 2, slowly dropping 5mL of 4.52mg/mL aqueous solution of gallic acid into the above mixed solution and continuing stirring for 2 hours to obtain a pale purple liquid.
And 3, dialyzing the light purple liquid for 2 hours, and removing sodium deoxycholate, unreacted iron ions and gallic acid to obtain the metal-polyphenol mesoporous nano material.
FIG. 2 is a UV absorption spectrum of glucose oxidase @ iron ion-gallic acid mesoporous nanomaterial (GOx @ Fe-GA) prepared in example 1, metal-polyphenol mesoporous nanomaterial (Fe-GA) prepared in example 2, and glucose oxidase (GOx). The test procedure was as follows: GOx @ Fe-GA, fe-GA and GOx are respectively diluted by 10 times, and an absorption spectrum at 300-800nm is read by an ultraviolet-visible spectrophotometer. As can be seen from FIG. 2, a broad absorption band in the range of 450-650nm occurs in the GOx @ Fe-GA and Fe-GA UV-visible spectra. This is typically catechol to Fe 3+ A charge transfer band. Therefore, the formation of Fe-GA is not affected even if glucose oxidase is added.
Example 3
Step 2, slowly dropping 5mL of 4.52mg/mL aqueous solution of gallic acid into the above mixed solution and continuing stirring for 2 hours to obtain a pale purple liquid.
And 3, dialyzing the light purple liquid for 2 hours, and removing phosphate, unreacted iron ions, gallic acid and glucose oxidase to obtain the glucose oxidase @ metal-polyphenol nano material with a non-mesoporous structure.
As a result: the glucose oxidase @ metal-polyphenol nano material with a non-mesoporous structure has the minimum inhibitory concentration of 7.42ug/mL on staphylococcus aureus, and shows a higher minimum inhibitory concentration compared with the glucose oxidase @ metal-polyphenol nano material with a mesoporous structure.
Example 4
The only difference from example 2 is that the gallic acid aqueous solution was replaced with different polyphenols: ellagic Acid (EA), tannic Acid (TA), epigallocatechin gallate (EGCG), caffeic Acid (CA), pyrogallic Acid (PA), to obtain mesoporous iron-polyphenol nanomaterial.
Fig. 8 is a graph showing the change of absorbance at 652nm of the iron ion-polyphenol mesoporous nanomaterials prepared in examples 2 and 4. The test procedure was as follows: the mesoporous fe ion-polyphenol nanomaterial prepared in this example (300 μ L,0.1 mmoL) was mixed with 3,3',5,5' -tetramethylbenzidine (300 μ L,20.0 mmoL), acetic acid buffer solution (2300 μ L, pH = 4.5), and H 2 O 2 (300. Mu.L, 25.0 mM) were mixed. It was then tested for uv-visible absorption in the 652nm range. As can be seen from FIG. 8, when gallic acid solution was used as the polyphenol source, the absorbance at 652nm was the highest.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (9)
1. A mesoporous nano material with an antibacterial function is characterized by comprising glucose oxidase @ metal-polyphenol, and having the structure as follows: the metal-polyphenol forms a network encapsulating the glucose oxidase.
2. The mesoporous nanomaterial with antibacterial function according to claim 1, wherein the glucose oxidase @ metal-polyphenol specifically is: glucose oxidase @ iron ion-gallic acid.
3. The preparation method of the mesoporous nano material with the antibacterial function according to claim 1, characterized by comprising the following steps:
mixing and reacting glucose oxidase, a metal ion source and a template, and then adding a polyphenol source for assembly to obtain the mesoporous nano material with the antibacterial function.
4. The production method according to claim 3, wherein the concentration of the glucose oxidase in the reaction system is 10 to 200. Mu.g/mL, the concentration of the template is 0.1 to 0.5mM, the concentration of the metal ion source is 0.05 to 0.2mM, and the concentration of the polyphenol source is 0.1 to 1.0mM.
5. The method according to claim 3, wherein the time of the mixing reaction is 15 to 120min; the assembling time is 2-12h; and after the assembly is finished, the method also comprises the step of removing the template and unreacted raw materials through ultrafiltration or dialysis.
6. The method of claim 3, wherein the source of metal ions is FeCl 3 ·6H 2 O; the template is a surfactant; the polyphenol source is one of gallic acid, ellagic acid, tannic acid, epigallocatechin gallate and caffeic acid.
7. The method of claim 6, wherein the surfactant is sodium deoxycholate.
8. The use of the mesoporous nanomaterial with antibacterial function of claim 1 in the preparation of antibacterial materials.
9. The use of the mesoporous nanomaterial with antibacterial function of claim 1 in killing bacteria.
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