CN115491298B - An online monitoring device for bioaerosol - Google Patents
An online monitoring device for bioaerosol Download PDFInfo
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- CN115491298B CN115491298B CN202211302709.8A CN202211302709A CN115491298B CN 115491298 B CN115491298 B CN 115491298B CN 202211302709 A CN202211302709 A CN 202211302709A CN 115491298 B CN115491298 B CN 115491298B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
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Abstract
The invention discloses an online monitoring device for biological aerosol, which not only can realize real-time online monitoring of toxicity of the aerosol, but also can realize cleaning rapidly so as to facilitate next rapid monitoring.
Description
Technical Field
The invention particularly relates to an online monitoring device for biological aerosol, and relates to the field of aerosol monitoring equipment.
Background
With the increasing importance of environmental air quality, the research on aerosols in environmental air is increasing, and because air contains a large amount of particles, the particles are generally main components of aerosols, and the particles often contain bioaerosol particles and other pollutants composed of bacteria, fungi, viruses, animal and plant debris and the like, the bioaerosols have more direct and important effects on human health and public safety than other non-particles. Therefore, it is important to check the toxicity of aerosols in air. Currently, a plurality of detection methods for biological aerosol are more accurate and convenient, namely on-line detection of the aerosol, which comprises a method for estimating the content of microorganisms in the air by using specific wave band fluorescence generated by exciting light to excite biological macromolecules based on fluorescence excitation principles of biological macromolecules such as riboflavin, reduced coenzyme NADH and the like, wherein the ATP biochemical luminescence reaction method can more reliably indicate the microorganisms with actual biological activity, therefore, the method has more application, for example, CN111763614A discloses an on-line detection system and method for the biological aerosol based on ATP biochemical luminescence, the method can reduce the problem of false negative caused by agglomeration of particles and can facilitate automatic control of unmanned detection, however, the device can not realize continuous on-line detection, or the detection results of the front side and the rear side can be greatly influenced by each other.
Disclosure of Invention
Accordingly, in order to solve the above-mentioned shortcomings, the present invention provides an online monitoring device for bioaerosols.
The invention is realized by the way, an on-line monitoring device for biological aerosol is constructed, the on-line monitoring device comprises a first liquid collecting pipe, a second liquid collecting pipe, an agitating mechanism and a circulating cleaning mechanism, wherein the first liquid collecting pipe and the second liquid collecting pipe are both arranged on a monitoring seat, the agitating mechanism is further arranged at the bottom side of the monitoring seat and stretches into the first liquid collecting pipe and the second liquid collecting pipe so as to agitate solution in the first liquid collecting pipe and the second liquid collecting pipe, the first liquid collecting pipe and the second liquid collecting pipe are connected to a rotational flow sampling device in a parallel mode, a high-flow fan is arranged on the rotational flow sampling device, the first liquid collecting pipe and the second liquid collecting pipe are further connected with a liquid supplementing pipe in a parallel mode, the liquid supplementing pipe is further connected to a monitoring pipe in a parallel mode through a peristaltic pump, the end part of the monitoring pipe is connected to a monitoring instrument, a peristaltic pump is further arranged on the monitoring seat, and the circulating cleaning mechanism is further connected to the monitoring seat, and the circulating cleaning mechanism can clean the first liquid collecting pipe and the second liquid collecting pipe in a period after the monitoring of the first liquid collecting pipe and the second liquid collecting pipe is completed.
Further, as the preference, the ambient air is collected fast by the drive of large-traffic fan, carries the separation liquid after the whirl behind the whirl sampling device separation to first collector tube, in the second collector tube, and in the solution in the fluid infusion bottle is supplied to first collector tube, second collector tube through peristaltic pump to can stir by stirring mechanism, the solution in first collector tube, the second collector tube is carried to the monitoring instrument through peristaltic pump.
Further, as the preference, stirring mechanism includes miniature stirring motor, stirring roller and stirring axle, the below of monitoring seat is provided with the driving chamber, the symmetry is provided with two miniature stirring motors in the driving chamber, miniature stirring motor's output is connected with the stirring axle, the stirring axle upwards stretches into in the first liquid collecting or the second liquid collecting pipe, and can be in first liquid collecting or the rotation in the second liquid collecting pipe, the stirring axle stretches into the part of first liquid collecting pipe or the second liquid collecting pipe is equipped with the stirring brush around the wall cover.
Further, as the preference, circulation wiper mechanism includes base, pump package driving motor, circulating pump, washing liquid storage box, connection liquid pipe, circulation feed liquor pipe, circulation drain pipe and waste liquid case, fixedly on the base be provided with pump package driving motor, washing liquid storage box and waste liquid case, still be provided with on the base by pump package driving motor driven circulating pump, the feed liquor end of circulating pump is connected to washing liquid storage box through connection liquid pipe, the output of circulating pump is connected to first collector pipe and second collector pipe through circulation feed liquor pipe, the one end of circulation drain pipe is connected to first collector pipe and second collector pipe, the other end of circulation drain pipe is connected to the waste liquid case, still be provided with the suction pump on the circulation drain pipe.
Further, as the preference, first collector tube and second collector tube are split type structure, and all include the liquid collecting cavity, go up sleeve pipe, butt joint lid, the upper end of monitoring seat is provided with two liquid collecting cavities of symmetry, the upper end detachable seal in liquid collecting cavity is provided with the sleeve pipe, go up sheathed tube upper end an organic whole and be provided with the butt joint lid.
Further, preferably, the docking cover is provided with a plurality of connection ports, the liquid supplementing pipe and the branch pipe are connected to the respective connection ports, and the circulating liquid inlet pipe and the circulating liquid outlet pipe are connected to the liquid collecting cavity.
Further, preferably, a plurality of liquid spray heads are arranged on one side of the side wall of the liquid collecting cavity in an array mode, and the liquid spray heads are communicated with the circulating liquid inlet pipe.
Further, preferably, a liquid suction nozzle is arranged on the other side of the side wall of the liquid collecting cavity in an array mode, and the circulating liquid outlet pipe is communicated with the liquid suction nozzle.
Further, preferably, the circulation cleaning mechanism further includes a controller that controls the pump group driving motor to act so that the pump group driving motor can cause the circulation pump to pump the cleaning liquid in a pulse pressure manner when the circulation cleaning is performed, and the controller can also control the agitation mechanism to perform agitation when the cleaning is performed.
Further, preferably, the controller is further capable of controlling the suction pump to suck in a pulse pressure manner.
The invention has the following advantages: compared with the same type of equipment, the online monitoring device for the biological aerosol has the following advantages:
The on-line monitoring device for the biological aerosol can not only realize the real-time on-line monitoring of the toxicity of the aerosol, but also realize the cleaning rapidly so as to facilitate the next rapid monitoring.
Drawings
FIG. 1 is a schematic diagram of the front view of the present invention;
FIG. 2 is a schematic diagram of a front view of a monitor stand according to the present invention;
FIG. 3 is a schematic three-dimensional structure of a monitor stand according to the present invention;
FIG. 4 is a schematic diagram of the structure of an on-line monitoring system for ATP biochemical luminescence based bioaerosol in the background of the invention.
Detailed Description
The following detailed description of the present invention, taken in conjunction with fig. 1-4, clearly and completely describes the technical solutions of embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides an online monitoring device for biological aerosol through improvement, which comprises a first liquid collecting pipe A, a second liquid collecting pipe B, an agitating mechanism and a circulating cleaning mechanism, wherein the first liquid collecting pipe A and the second liquid collecting pipe B are respectively arranged on a monitoring seat 1, the agitating mechanism is further arranged at the bottom side of the monitoring seat 1 and extends into the first liquid collecting pipe A and the second liquid collecting pipe B so as to agitate solution in the first liquid collecting pipe A and the second liquid collecting pipe B, the first liquid collecting pipe A and the second liquid collecting pipe B are connected to a rotational flow sampling device in a parallel connection mode, a large-flow fan is arranged on the rotational flow sampling device, the first liquid collecting pipe and the second liquid collecting pipe are respectively connected with a liquid supplementing pipe in a parallel connection mode, the liquid supplementing pipes are respectively connected to a monitoring pipe in a parallel connection mode through a branch pipe, the end parts of the monitoring pipes are respectively connected to the monitoring pipes, peristaltic pumps are respectively arranged on the branch pipes (the peristaltic pumps are respectively arranged in the upper part and the monitoring pipes, the first liquid collecting pipe and the second liquid collecting pipe B are respectively connected with the monitoring seat in a parallel connection mode, and the circulating cleaning mechanism is used for cleaning the liquid collecting pipe B is convenient for cleaning the liquid collecting pipe A and the circulating device.
According to the invention, the ambient air is rapidly collected by a high-flow fan, the cyclone liquid is separated by the cyclone sampling device and then is conveyed into the first liquid collecting pipe and the second liquid collecting pipe, the solution in the liquid supplementing bottle is supplemented into the first liquid collecting pipe and the second liquid collecting pipe through the peristaltic pump and can be stirred by the stirring mechanism, and the solution in the first liquid collecting pipe and the solution in the second liquid collecting pipe are conveyed to the monitoring instrument through the peristaltic pump. The solution in the fluid infusion bottle is a reaction solution containing reaction ions such as cell lysate, luciferase, luciferin, magnesium ions and the like, and the reaction solution reacts with living microorganisms enriched in aerosol collected in the first fluid infusion tube and the second fluid infusion tube to lyse the microbial cells so as to facilitate the monitoring of the toxicity of the microorganisms by the monitoring instrument.
The stirring mechanism comprises a miniature stirring motor 11, a stirring roller 3 and a stirring shaft, a driving cavity 14 is arranged below the monitoring seat 1, two miniature stirring motors 11 are symmetrically arranged in the driving cavity 14, the output end of the miniature stirring motor 11 is connected with the stirring shaft, the stirring shaft stretches into the first liquid collecting pipe or the second liquid collecting pipe upwards and can rotate in the first liquid collecting pipe or the second liquid collecting pipe, and stirring brushes are sleeved on the peripheral walls of the part, stretching into the first liquid collecting pipe or the second liquid collecting pipe, of the stirring shaft.
The circulating cleaning mechanism comprises a base, a pump set driving motor 7, a circulating pump 9, a cleaning liquid storage box 8, a connecting liquid pipe 6, a circulating liquid inlet pipe 10, a circulating liquid outlet pipe 4 and a waste liquid box 5, wherein the pump set driving motor 7, the cleaning liquid storage box 8 and the waste liquid box 5 are fixedly arranged on the base, the circulating pump 9 driven by the pump set driving motor 7 is further arranged on the base, the liquid inlet end of the circulating pump 9 is connected to the cleaning liquid storage box 8 through the connecting liquid pipe 6, the output end of the circulating pump is connected to a first liquid collecting pipe A and a second liquid collecting pipe B through the circulating liquid inlet pipe 10, one end of the circulating liquid outlet pipe 4 is connected to the first liquid collecting pipe and the second liquid collecting pipe, the other end of the circulating liquid outlet pipe 4 is connected to the waste liquid box 5, and a suction pump is further arranged on the circulating liquid outlet pipe 4.
The first liquid collecting pipe and the second liquid collecting pipe are of split type structures and comprise a liquid collecting cavity 15, an upper sleeve 16 and a butt joint cover 2, two symmetrical liquid collecting cavities 15 are arranged at the upper end of the monitoring seat 1, the upper sleeve 16 is arranged at the upper end of the liquid collecting cavity 15 in a detachable sealing mode, and the butt joint cover 2 is integrally arranged at the upper end of the upper sleeve 16.
The butt joint cover 2 is provided with a plurality of connectors 17, the liquid supplementing pipes and the branch pipes are connected to the connectors 17, and the circulating liquid inlet pipe and the circulating liquid outlet pipe are connected to the liquid collecting cavity.
A plurality of liquid spray heads 13 are arranged on one side of the side wall of the liquid collecting cavity in an array mode, and the liquid spray heads are communicated with the circulating liquid inlet pipe 10.
The other side of the side wall of the liquid collecting cavity is provided with liquid sucking nozzles 12 in an array mode, and the circulating liquid outlet pipe 4 is communicated with the liquid sucking nozzles 12.
In this embodiment, the circulation cleaning mechanism further includes a controller that controls the pump group driving motor to act so that the pump group driving motor can cause the circulation pump to pump the cleaning liquid in a pulse pressure manner when the circulation cleaning is performed, and the controller can also control the agitation mechanism to agitate when the cleaning is performed.
As a preferred embodiment, the controller is further capable of controlling the suction pump to suck in a pulsed pressure manner.
The invention not only can realize real-time online monitoring of the toxicity of aerosol, but also can realize cleaning rapidly so as to facilitate the next rapid monitoring, the invention can realize online continuous monitoring, also can realize online intermittent monitoring with a certain period, and improves the monitoring convenience.
The basic principle and main characteristics of the invention and the advantages of the invention are shown and described above, standard parts used by the invention can be purchased from market, special-shaped parts can be customized according to the description of the specification and the drawings, the specific connection modes of the parts adopt conventional means such as mature bolt rivets and welding in the prior art, the machinery, the parts and the equipment adopt conventional models in the prior art, and the circuit connection adopts conventional connection modes in the prior art, so that the description is omitted.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. The on-line monitoring device for the biological aerosol comprises a first liquid collecting pipe, a second liquid collecting pipe, an agitating mechanism and a circulating cleaning mechanism, wherein the first liquid collecting pipe and the second liquid collecting pipe are respectively arranged on a monitoring seat;
The circulating cleaning mechanism comprises a base, a pump set driving motor, a circulating pump, a cleaning liquid storage tank, a connecting liquid pipe, a circulating liquid inlet pipe, a circulating liquid outlet pipe and a waste liquid tank, wherein the pump set driving motor, the cleaning liquid storage tank and the waste liquid tank are fixedly arranged on the base, the circulating pump driven by the pump set driving motor is further arranged on the base, the liquid inlet end of the circulating pump is connected to the cleaning liquid storage tank through the connecting liquid pipe, the output end of the circulating pump is connected to a first liquid collecting pipe and a second liquid collecting pipe through the circulating liquid inlet pipe, one end of the circulating liquid outlet pipe is connected to the first liquid collecting pipe and the second liquid collecting pipe, the other end of the circulating liquid outlet pipe is connected to the waste liquid tank, and a suction pump is further arranged on the circulating liquid outlet pipe;
The first liquid collecting pipe and the second liquid collecting pipe are of split type structures and comprise liquid collecting cavities, an upper sleeve and a butt joint cover, two symmetrical liquid collecting cavities are arranged at the upper end of the monitoring seat, the upper sleeve is detachably arranged at the upper end of the liquid collecting cavity in a sealing mode, and the butt joint cover is integrally arranged at the upper end of the upper sleeve;
a plurality of liquid spray heads are arranged on one side of the side wall of the liquid collecting cavity in an array manner, and the liquid spray heads are communicated with the circulating liquid inlet pipe;
the other side of the side wall of the liquid collecting cavity is provided with liquid suction nozzles in an array mode, and the circulating liquid outlet pipe is communicated with the liquid suction nozzles.
2. An on-line bioaerosol monitoring apparatus as defined in claim 1, wherein: ambient air is rapidly collected by the drive of large-traffic fan, carries the separation liquid after the whirl behind the whirl sampling device separation to first collector tube, in the second collector tube, and the solution in the fluid infusion bottle is supplied to first collector tube, in the second collector tube through peristaltic pump to can stir by stirring mechanism, the solution in first collector tube, the second collector tube is carried to the monitoring instrument through peristaltic pump.
3. An on-line bioaerosol monitoring apparatus as defined in claim 1, wherein: the stirring mechanism comprises a miniature stirring motor, a stirring roller and a stirring shaft, a driving cavity is arranged below the monitoring seat, two miniature stirring motors are symmetrically arranged in the driving cavity, the output end of each miniature stirring motor is connected with the stirring shaft, the stirring shaft stretches into the first liquid collecting pipe or the second liquid collecting pipe upwards and can rotate in the first liquid collecting pipe or the second liquid collecting pipe, and stirring brushes are sleeved on the peripheral walls of the part, stretching into the first liquid collecting pipe or the second liquid collecting pipe, of the stirring shaft.
4. An on-line bioaerosol monitoring apparatus as defined in claim 3, wherein: the butt joint cover is provided with a plurality of connectors, the liquid supplementing pipe and the branch pipe are connected to the connectors respectively, and the circulating liquid inlet pipe and the circulating liquid outlet pipe are connected to the liquid collecting cavity.
5. An on-line bioaerosol monitoring apparatus as defined in claim 1, wherein: the circulating cleaning mechanism further comprises a controller which controls the pump set driving motor to act, so that when circulating cleaning is carried out, the pump set driving motor can enable the circulating pump to pump cleaning liquid in a pulse pressure mode, and when cleaning is carried out, the controller can also control the stirring mechanism to stir.
6. An on-line bioaerosol monitoring apparatus as defined in claim 5, wherein: the controller is also capable of controlling the suction pump to pump in a pulsed pressure manner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202211302709.8A CN115491298B (en) | 2022-10-24 | 2022-10-24 | An online monitoring device for bioaerosol |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211302709.8A CN115491298B (en) | 2022-10-24 | 2022-10-24 | An online monitoring device for bioaerosol |
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| CN115491298A CN115491298A (en) | 2022-12-20 |
| CN115491298B true CN115491298B (en) | 2024-05-24 |
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