CN115487249A - Preparation method of isatis root and folium isatidis granules - Google Patents
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- CN115487249A CN115487249A CN202210951277.7A CN202210951277A CN115487249A CN 115487249 A CN115487249 A CN 115487249A CN 202210951277 A CN202210951277 A CN 202210951277A CN 115487249 A CN115487249 A CN 115487249A
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Abstract
The invention relates to a preparation method of isatis root and folium isatidis granules. The radix Isatidis granule is prepared from radix Isatidis and folium Isatidis. Has the functions of clearing away heat and toxic material and cooling blood. It can be used for treating wind-heat type common cold, sore throat, and warm-heat type diseases such as fever and speckle. Although the isatis root granules are simple in medicinal material composition and convenient to prepare, the extraction conditions of the active ingredients of isatis root and isatis leaf are different, and the active ingredients extracted at high temperature for a long time are easy to decompose, so that the isatis leaf granules are extracted by alcohol and the isatis root is extracted by water, and the temperature is strictly controlled. The invention designs a scientific and reasonable preparation method, and can ensure that the content of the effective component (R, S) -goitrin in each batch of indigowoad leaf granules is kept at about 120 mu g/g and the content of the indirubin is kept at about 70 mu g/g which is far higher than the average level while the steam dosage is reduced due to low extraction temperature and short heating time.
Description
Technical Field
The invention relates to the technical field of veterinary medicines, in particular to a preparation method of isatis root-folium isatidis granules.
Background
The isatis root and isatis leaf granules are prepared by the steps of decocting, filtering, concentrating, granulating and the like of two medicinal materials of isatis root and isatis leaf. Has the functions of clearing away heat and toxic material and cooling blood. It can be used for treating wind-heat type common cold, sore throat, and warm-heat type diseases such as fever and speckle.
At present, the standard quality of isatis leaf granules requires a few indexes, only physicochemical identification and thin-layer identification are required, and the quantitative index requirements of active ingredients are not required. The quality standard requires less indexes, so that the content difference between the products of different manufacturers and the products of different batches of the same manufacturer is very large. Even a plurality of counterfeit and shoddy products appear, which is not beneficial to the standardization and the expanded production of the indigowoad root granules.
In order to solve the problems, relevant personnel intensively study the drug properties and effective components of the two medicinal materials of the isatis root and the indigowoad leaf, and add the detection of the effective components (R, S) -goitrin and indirubin of the isatis root granules on the basis of the qualitative detection of the isatis root granules. After a large number of experiments, the two effective components are easily decomposed under the condition of long-time decoction, and the indirubin is only extracted in a very small amount in the traditional water extraction method and can be extracted in a large amount in an ethanol solution. Therefore, the method for preparing the indigowoad root-bark particles is scientific, and the indigowoad root-bark particles which have higher effective components and controllable quality and are suitable for large-scale production are necessary.
Disclosure of Invention
The invention aims to provide a preparation method of isatis root-folium isatidis particles, and the isatis root-folium isatidis particles prepared by the method are stable in quality and extremely high in content of effective components, so that the production trend of the isatis root-folium isatidis particles is promoted to be digital and standardized.
In order to achieve the purpose, the invention designs the following scheme:
preparing 600 parts of isatis root medicinal material and 900 parts of folium isatidis medicinal material. The adjuvants are sucrose and dextrin.
The process for preparing the isatis root granules is as follows:
step 1: soaking radix Isatidis in water.
Step 2: taking the folium isatidis as a medicinal material, adding an ethanol solution for extraction, and collecting an extracting solution after extraction.
And 3, step 3: and (3) extracting the folium isatidis dregs extracted in the step (2) and the radix isatidis with soaking water together, and collecting an extracting solution.
And 4, step 4: and (3) mixing the extracting solutions in the step (2) and the step (3), centrifuging, and concentrating under reduced pressure to prepare an extract.
And 5: and (4) adding a proper amount of auxiliary materials into the extract prepared in the step (4) for granulation.
Preferably, in the step 1, the water for soaking the isatis root is 8-15 times of the total mass of the isatis root and the dyers woad leaf, and the soaking time is 1-3 hours, so that the isatis root is fully soaked for convenient extraction.
Preferably, the ethanol solution in the step 2 is 40 to 50 mass percent of ethanol solution, the dosage of the ethanol solution is 8 to 15 times of the mass of the folium isatidis, the extraction temperature is 75 to 85 ℃, and the extraction time is 0.5 to 1.5 hours.
Preferably, in step 3, the dregs of the extraction of the dyers woad leaf and the isatis root are extracted together, the soaking water of the isatis root is reserved, and the water also contains a large amount of (R, S) -goitrin.
Preferably, the reduced pressure concentration in the step 4 is carried out at a temperature of 50 ℃ to 60 ℃ and a pressure of-0.08 MPa to-0.1 MPa. Concentrating until the relative density of the extract is 1.33-1.35 (55 deg.C).
Preferably, in the step 5, the adding mass ratio of the extract, the dextrin and the sucrose is respectively 15-25%, 53-60% and 22-25%. The dextrin and the sucrose are uniformly mixed, then the extract is added, and the three are stirred until the granulation condition is met.
Preferably, the wet particle drying method in step 5 is oven drying at a temperature of 40 ℃ to 50 ℃.
Compared with the prior art, the invention has the advantages that:
the method has the advantages that: the content of (R, S) -goitrin in the prepared indigowoad root granules is 1.5 to 3 times of that of indigowoad root granules on the market, and the content of most of (R, S) -goitrin in the indigowoad root granules on the market is 30 to 70 mu g/g, and the content of the (R, S) -goitrin in the indigowoad root granules is about 120 mu g/g.
The method has the advantages that: the content of indirubin in the indigowoad grains prepared by the invention is 1.5-2 times of that of indigowoad grains on the market, and the content of the indirubin in most indigowoad grains on the market is about 40 mu g/g, and the content of the indirubin in the indigowoad grains is about 70 mu g/g.
The method has the advantages that: the isatis root-folium isatidis granules prepared by the method have stable quality and basically unchanged effective component content under the condition that medicinal materials are used in the same batch, and are favorable for digitalizing and standardizing the trend of the isatis root-folium isatidis granules.
The advantages are that: compared with the conventional preparation method, the preparation method disclosed by the invention has the advantages that the extraction temperature is reduced by 20 ℃, so that the energy consumption in the process of producing the indigowoad root particles is reduced, and the purposes of energy conservation and emission reduction are achieved.
Drawings
FIG. 1 is a high performance liquid chromatogram of effective component (R, S) -goitrin in Banqing granule.
FIG. 2 is a high performance liquid chromatogram of the effective component indirubin in Banqing granules.
FIG. 3 shows the conditions for extraction of (R, S) -goitrin.
FIG. 4 shows the indirubin extraction conditions.
Detailed Description
The present invention will be described in detail with reference to specific examples, which are not intended to limit the present invention but are merely illustrative thereof. The scope of the invention is defined by the appended claims. The medicinal materials, reagents and reference substances used in the examples are all purchased from the market without special instructions.
Example 1: and (3) detecting the content of the effective component (R, S) -goitrin in the isatis root granules.
Chromatographic conditions and system applicability test: octadecylsilane chemically bonded silica (C18 chromatographic column) is used as a filler; methanol-0.02% phosphoric acid solution (7; the detection wavelength was 245nm. The number of theoretical plates is not less than 5000 according to the calculation of (R, S) -goitrin peak.
Preparation of control solutions: accurately weighing appropriate amount of (R, S) -goitrin reference substance, and adding methanol to obtain solution containing 40 μ g per 1 ml.
Preparation of a test solution: grinding the isatis root granules into fine powder (sieving by a No. four sieve), weighing about 1g, and precisely weighing. Adding appropriate amount of methanol, ultrasonic treating for 30 min, cooling, diluting to 50ml, shaking, filtering, and collecting filtrate.
Precisely sucking 10 μ l of each of the reference solution and the sample solution, injecting into liquid chromatograph, and measuring.
The high performance liquid chromatogram is shown in the attached figure of the specification, and figure 1 is the high performance liquid chromatogram of effective components (R, S) -goitrin in Banqing granules.
Example 2: and detecting the content of the effective component indirubin in the isatis root granules.
Chromatographic conditions and system applicability test: octadecylsilane chemically bonded silica (C18 chromatographic column) is used as a filler; methanol-water (75; the detection wavelength is 289nm. The number of theoretical plates should be not less than 4000 in terms of indirubin peak.
Preparation of control solutions: taking a proper amount of indirubin reference substance, precisely weighing, and adding methanol to obtain a solution containing 2 μ g per 1 ml.
Preparation of a test solution: grinding the isatis root granules into fine powder (sieving by a No. four sieve), weighing about 1g, and precisely weighing. Adding appropriate amount of methanol, ultrasonic treating for 30 min, cooling, diluting to 50ml, shaking, filtering, and collecting filtrate.
The determination method comprises the following steps: precisely sucking 20 μ l of each of the reference solution and the sample solution, injecting into liquid chromatograph, and measuring.
The high performance liquid chromatogram is shown in the attached figure of the specification, and figure 2 is the high performance liquid chromatogram of the effective component indirubin in the isatis root granules.
Example 3: and (R, S) -goitrin extraction condition screening.
Taking 6 500mL round-bottom flasks, and respectively adding 20g of isatis root with the number of 1-6. 1. No. 4 with water 200mL, no. 2, no. 5 with 50% ethanol 200mL, no. 3, no. 6 with 70% ethanol 200mL. No. 1-3 is placed in a water bath at 60 ℃, and No. 4-6 is placed in a water bath at 80 ℃. Sampling and detecting every 1h.
The results of the (R, S) -goitrin extraction are shown in FIG. 3.
As can be seen from FIG. 3, the optimum extraction temperature of (R, S) -goitrin was 80 ℃ and the time was 2 hours. The content of (R, S) -goitrin is almost 0 by detecting the isatis root dregs under the condition, so that the (R, S) -goitrin can be completely extracted by judging at 80 ℃, and the conventional decoction condition is not needed, thereby saving a large amount of energy.
Example 4: and (4) screening the indirubin extraction conditions.
Taking 6 500mL round-bottom flasks, and respectively adding 20g of folium isatidis, wherein the number is 1-6. 1. No. 4 with water 200mL, no. 2, no. 5 with 50% ethanol 200mL, no. 3, no. 6 with 70% ethanol 200mL. No. 1-3 is placed in water bath at 60 deg.C, and No. 4-6 is placed in water bath at 80 deg.C. Sampling and detecting every 1h.
The indirubin extraction results are shown in fig. 4.
From the results of fig. 4, it can be seen that the optimum extraction time of indirubin is 1 hour, and the optimum temperature is 80 ℃. And the indirubin is decomposed after being extracted at high temperature for a long time, so the extraction time and temperature should be controlled. The content of indirubin in folium Isatidis dregs after extraction under the optimum condition is below the detection limit, and the indirubin can be considered to be extracted completely.
As can be seen from examples 3 and 4, the conventional extraction method has a great problem in that indirubin cannot be extracted and the extraction temperature of (R, S) -goitrin is too high, which causes energy waste.
Example 5: azure particles were prepared according to the method of the invention.
Taking 120g of radix isatidis and 180g of folium isatidis. 196g of dextrin and 84g of cane sugar (after being crushed) as auxiliary materials.
Soaking radix Isatidis in 3000ml water at room temperature for 2 hr.
Adding folium Isatidis into 45% ethanol solution, and extracting at 80 deg.C for 1h. Collecting the extract after extraction.
Extracting the extracted folium Isatidis residue with radix Isatidis and soaking water at 80 deg.C for 2 hr, and collecting the extractive solution.
The collected extracts were mixed well and centrifuged, concentrated at-0.1MPa and 55 ℃ to give an extract with a relative density of 1.35 (55 ℃), amounting to 73.3g.
Mixing dextrin and sucrose uniformly (about ten minutes for stirring), slowly adding the extract into the adjuvant, stirring the soft material until the soft material is agglomerated, and dispersing when the soft material is contacted.
Granulating the soft material by using a rotary granulator, and drying the wet granules in an oven at 45 ℃ to finally obtain 295.3g of indigowoad leaf granules.
The content of the indigowoad leaf granules was measured by the method of example 1 and example 2, and the (R, S) -goitrin content was 118.6. Mu.g/g, and the indirubin content was 71.7. Mu.g/g.
Example 5 was repeated several times using the same batch of crude drug and the content of active ingredient of azure granules was measured every time. The results are within the error tolerance range, and the repeatability of the method is extremely high.
The content of (R, S) -goitrin particles in common isatis leaf granules in the market is about 30-70 mu g/g, the content of (R, S) -goitrin in partial granules is 20-40 mu g/g even under the detection limit, and the content of partial granules is below the detection limit. The isatis root granules contain about 120 mu g/g of (R, S) -goitrin and about 70 mu g/g of indirubin. As can be seen by comparison, the isatis root granules prepared by the method have stable content, the content of (R, S) -goitrin in the isatis root granules is 1.5-3 times that of the isatis root granules in the market, and the content of indirubin in the isatis root granules is 1.5-2 times that of the isatis root granules in the market.
Claims (6)
1. The preparation method of the isatis root-folium isatidis granules is characterized by comprising the following steps of:
step 1: soaking radix Isatidis in water.
Step 2: taking the folium isatidis as a medicinal material, adding an ethanol solution for extraction, and collecting an extracting solution after extraction.
And 3, step 3: and (3) extracting the folium isatidis dregs extracted in the step (2) and the radix isatidis with soaking water together, and collecting an extracting solution.
And 4, step 4: and (3) mixing the extracting solutions in the step (2) and the step (3), centrifuging, and concentrating under reduced pressure to prepare an extract.
And 5: and (4) adding a proper amount of auxiliary materials into the extract prepared in the step (4) for granulation.
2. The preparation method of isatis root-isatis root granules according to claim 1, characterized in that the amount of water added for soaking the isatis root in the step 1 is 8-15 times of the total mass of the isatis root and the isatis leaf, and the soaking time is 1-3 hours.
3. The method for preparing isatis root granules according to claim 1, wherein the ethanol solution in step 2 is 40-50% by mass, and the amount of the ethanol solution added is 8-15 times of the mass of the folium isatidis. The extraction temperature is 75-85 ℃, and the extraction time is 0.5-1.5 hours.
4. The method for preparing isatis root-folium isatidis granules according to claim 1, wherein the temperature for co-extraction of isatis root and folium isatidis in step 3 is 75-85 ℃ for 1-3 hours.
5. The process for producing azure granules according to claim 1, characterized in that in step 4, the vacuum degree of concentration under reduced pressure is-0.08 to-0.1 MPa and the temperature is 50 ℃ to 60 ℃. Concentrating until the relative density of the extract is 1.30-1.35 (55 deg.C).
6. The method for preparing isatis root granules according to claim 1, wherein in the step 5, the auxiliary materials are dextrin and sucrose. The mass ratio of the extract, the dextrin and the sucrose is respectively 15-25%, 53-60% and 22-25%.
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| CN117815319A (en) * | 2023-10-26 | 2024-04-05 | 广州白云山和记黄埔中药有限公司 | Compound isatis root preparation, preparation method thereof and application thereof in resisting influenza |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899349A (en) * | 2006-07-04 | 2007-01-24 | 贵州百花医药股份有限公司 | Compound isatis root effervescence tablet and its preparing method |
| CN102727545A (en) * | 2012-06-30 | 2012-10-17 | 郑州百瑞动物药业有限公司 | Preparation method of indigowoad root and indigowoad leaf particles used for livestock and poultry |
| CN104173601A (en) * | 2014-08-20 | 2014-12-03 | 赵燕翼 | Radix isatidis and folium isatidis tablet and preparation method thereof |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899349A (en) * | 2006-07-04 | 2007-01-24 | 贵州百花医药股份有限公司 | Compound isatis root effervescence tablet and its preparing method |
| CN102727545A (en) * | 2012-06-30 | 2012-10-17 | 郑州百瑞动物药业有限公司 | Preparation method of indigowoad root and indigowoad leaf particles used for livestock and poultry |
| CN104173601A (en) * | 2014-08-20 | 2014-12-03 | 赵燕翼 | Radix isatidis and folium isatidis tablet and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 吴国军 等: "基于表告依春的板蓝根水提正交试验研究", 《黑龙江八一农垦大学学报》, vol. 26, no. 6, pages 29 - 33 * |
| 和翀翼 等: "大青叶中靛玉红提取工艺的研究", 《黑龙江畜牧兽医》, no. 3, pages 53 - 54 * |
| 张前珍 等: "复方板蓝根制剂中靛玉红提取工艺的研究", 《安徽卫生职业技术学院学报》, vol. 19, no. 6, pages 93 - 95 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117815319A (en) * | 2023-10-26 | 2024-04-05 | 广州白云山和记黄埔中药有限公司 | Compound isatis root preparation, preparation method thereof and application thereof in resisting influenza |
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