CN115485602A - 显微镜系统、投影单元以及精子筛选辅助方法 - Google Patents

显微镜系统、投影单元以及精子筛选辅助方法 Download PDF

Info

Publication number
CN115485602A
CN115485602A CN202180025391.0A CN202180025391A CN115485602A CN 115485602 A CN115485602 A CN 115485602A CN 202180025391 A CN202180025391 A CN 202180025391A CN 115485602 A CN115485602 A CN 115485602A
Authority
CN
China
Prior art keywords
sperm
image
microscope
information
microscope system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180025391.0A
Other languages
English (en)
Chinese (zh)
Inventor
木村円香
山根拓人
服部敏征
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yijingtong Co ltd
Original Assignee
Yijingtong Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yijingtong Co ltd filed Critical Yijingtong Co ltd
Publication of CN115485602A publication Critical patent/CN115485602A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20081—Training; Learning
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20084—Artificial neural networks [ANN]

Landscapes

  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Multimedia (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Dispersion Chemistry (AREA)
  • Signal Processing (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Image Analysis (AREA)
CN202180025391.0A 2020-03-31 2021-03-10 显微镜系统、投影单元以及精子筛选辅助方法 Pending CN115485602A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020063284 2020-03-31
JP2020-063284 2020-03-31
PCT/JP2021/009685 WO2021200003A1 (ja) 2020-03-31 2021-03-10 顕微鏡システム、投影ユニット、及び、精子選別支援方法

Publications (1)

Publication Number Publication Date
CN115485602A true CN115485602A (zh) 2022-12-16

Family

ID=77928722

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180025391.0A Pending CN115485602A (zh) 2020-03-31 2021-03-10 显微镜系统、投影单元以及精子筛选辅助方法

Country Status (4)

Country Link
EP (1) EP4130843A4 (https=)
JP (1) JPWO2021200003A1 (https=)
CN (1) CN115485602A (https=)
WO (1) WO2021200003A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022192436A1 (en) 2021-03-09 2022-09-15 Thread Robotics Inc. System and method for automated gamete selection
US11694344B2 (en) 2021-11-05 2023-07-04 Thread Robotics Inc. System and method for automated cell positioning
JP7693533B2 (ja) * 2021-12-27 2025-06-17 株式会社エビデント 顕微鏡システム、投影ユニット、及び、選別支援方法
US20230399598A1 (en) * 2022-06-08 2023-12-14 Ipreg Incorporation. Vertical thermal gradient equipment and method for progressive sperm sorting
WO2023248958A1 (ja) * 2022-06-24 2023-12-28 株式会社エビデント 顕微鏡システム、投影ユニット、選別支援方法、及び、記録媒体
CN115074237B (zh) * 2022-07-29 2024-09-27 贵州省畜牧兽医研究所 一种山羊育种优选精子的显微操作取出采集装置
EP4605726A4 (en) 2022-10-17 2026-01-14 Vitruvianmd Pte Ltd VISUAL ANALYSIS OF SPERM DNA FRAGMENTATION
TWI876619B (zh) * 2023-10-25 2025-03-11 國立陽明交通大學 精蟲分選裝置
JP7580169B1 (ja) 2024-09-09 2024-11-11 株式会社アークス 精子選別補助装置、精子選別補助システム、及び精子選別補助プログラム
JP7751345B1 (ja) * 2025-04-24 2025-10-08 株式会社アークス 精子選別補助装置、投影装置、及び顕微鏡システム

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012198249A (ja) * 2012-07-27 2012-10-18 Chiba Univ 精子機能の検査方法
JP2017092730A (ja) * 2015-11-11 2017-05-25 ソニー株式会社 情報処理装置、情報処理方法、プログラム及び情報処理システム
WO2020008920A1 (ja) * 2018-07-02 2020-01-09 ソニー株式会社 医療用観察システム、医療用観察装置、及び医療用観察装置の駆動方法
WO2020059522A1 (ja) * 2018-09-21 2020-03-26 公立大学法人横浜市立大学 生殖医療支援システム
CN112714888A (zh) * 2018-09-28 2021-04-27 奥林巴斯株式会社 显微镜系统、投影单元以及图像投影方法
CN112714887A (zh) * 2018-09-28 2021-04-27 奥林巴斯株式会社 显微镜系统、投影单元以及图像投影方法
CN113260894A (zh) * 2018-12-28 2021-08-13 奥林巴斯株式会社 显微镜系统
CN116736512A (zh) * 2022-03-08 2023-09-12 仪景通株式会社 显微镜系统、叠加单元以及动作方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0829694A (ja) * 1994-07-20 1996-02-02 Nikon Corp 画像処理装置付き顕微鏡
JPWO2012150689A1 (ja) 2011-05-02 2014-07-28 オリンパス株式会社 顕微鏡、及び、顕微鏡を用いた顕微授精方法

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012198249A (ja) * 2012-07-27 2012-10-18 Chiba Univ 精子機能の検査方法
JP2017092730A (ja) * 2015-11-11 2017-05-25 ソニー株式会社 情報処理装置、情報処理方法、プログラム及び情報処理システム
CN108351919A (zh) * 2015-11-11 2018-07-31 索尼公司 信息处理设备、信息处理方法、程序及信息处理系统
WO2020008920A1 (ja) * 2018-07-02 2020-01-09 ソニー株式会社 医療用観察システム、医療用観察装置、及び医療用観察装置の駆動方法
WO2020059522A1 (ja) * 2018-09-21 2020-03-26 公立大学法人横浜市立大学 生殖医療支援システム
CN112714888A (zh) * 2018-09-28 2021-04-27 奥林巴斯株式会社 显微镜系统、投影单元以及图像投影方法
CN112714887A (zh) * 2018-09-28 2021-04-27 奥林巴斯株式会社 显微镜系统、投影单元以及图像投影方法
CN113260894A (zh) * 2018-12-28 2021-08-13 奥林巴斯株式会社 显微镜系统
CN116736512A (zh) * 2022-03-08 2023-09-12 仪景通株式会社 显微镜系统、叠加单元以及动作方法

Also Published As

Publication number Publication date
WO2021200003A1 (ja) 2021-10-07
EP4130843A4 (en) 2024-05-01
EP4130843A9 (en) 2024-06-05
JPWO2021200003A1 (https=) 2021-10-07
EP4130843A1 (en) 2023-02-08

Similar Documents

Publication Publication Date Title
CN115485602A (zh) 显微镜系统、投影单元以及精子筛选辅助方法
CN113260894B (zh) 显微镜系统
Louis et al. Review of computer vision application in in vitro fertilization: the application of deep learning-based computer vision technology in the world of IVF
US10843190B2 (en) Apparatus and method for analyzing a bodily sample
CN110431463A (zh) 组织样本的组织学检查系统
EP3065105A1 (en) Technique for determining the state of a cell aggregation, image processing program and image processing device using the technique, and method for producing a cell aggregation
US9145572B2 (en) Observation system, recording medium, and control method of observation system
JP7253273B2 (ja) 生殖医療支援システム
CN114127307A (zh) 细胞图像解析方法和细胞图像解析装置
CN115552309A (zh) 显微镜系统、投影单元以及检卵辅助方法
US20250308267A1 (en) Automated training of a machine-learned algorithm on the basis of the monitoring of a microscopy measurement
WO2023248958A1 (ja) 顕微鏡システム、投影ユニット、選別支援方法、及び、記録媒体
Gill et al. Looking with new eyes: advanced microscopy and artificial intelligence in reproductive medicine
JP7751345B1 (ja) 精子選別補助装置、投影装置、及び顕微鏡システム
Brooks et al. CelFDrive: Artificial Intelligence assisted microscopy for automated detection of rare events
Sidhu Automated Blastomere Segmentation for Visual Servo on Early-stage Embryo
JP7693533B2 (ja) 顕微鏡システム、投影ユニット、及び、選別支援方法
WO2023127001A1 (ja) 顕微鏡システム、投影ユニット、及び、選別支援方法
Sergeev et al. Development of automated computer vision methods for cell counting and endometrial gland detection for medical images processing
JP2025158211A (ja) 顕微鏡システム
JP2024059329A (ja) 顕微鏡システム
Mulani et al. AN ARTIFICIAL INTELLIGENCE APPROACH TO DETECT AND CLASSIFY DNA INTEGRITY OF SPERMS AND EGGS USING YOLO ALGORITHM

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20221216

WD01 Invention patent application deemed withdrawn after publication