CN115464511B - Device integrating multi-channel aperture detection and nanopore preparation and application method thereof - Google Patents
Device integrating multi-channel aperture detection and nanopore preparation and application method thereof Download PDFInfo
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- CN115464511B CN115464511B CN202211135546.9A CN202211135546A CN115464511B CN 115464511 B CN115464511 B CN 115464511B CN 202211135546 A CN202211135546 A CN 202211135546A CN 115464511 B CN115464511 B CN 115464511B
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- nanopore
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B24—GRINDING; POLISHING
- B24B—MACHINES, DEVICES, OR PROCESSES FOR GRINDING OR POLISHING; DRESSING OR CONDITIONING OF ABRADING SURFACES; FEEDING OF GRINDING, POLISHING, OR LAPPING AGENTS
- B24B19/00—Single-purpose machines or devices for particular grinding operations not covered by any other main group
- B24B19/16—Single-purpose machines or devices for particular grinding operations not covered by any other main group for grinding sharp-pointed workpieces, e.g. needles, pens, fish hooks, tweezers or record player styli
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B24—GRINDING; POLISHING
- B24B—MACHINES, DEVICES, OR PROCESSES FOR GRINDING OR POLISHING; DRESSING OR CONDITIONING OF ABRADING SURFACES; FEEDING OF GRINDING, POLISHING, OR LAPPING AGENTS
- B24B19/00—Single-purpose machines or devices for particular grinding operations not covered by any other main group
- B24B19/22—Single-purpose machines or devices for particular grinding operations not covered by any other main group characterised by a special design with respect to properties of the material of non-metallic articles to be ground
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B24—GRINDING; POLISHING
- B24B—MACHINES, DEVICES, OR PROCESSES FOR GRINDING OR POLISHING; DRESSING OR CONDITIONING OF ABRADING SURFACES; FEEDING OF GRINDING, POLISHING, OR LAPPING AGENTS
- B24B41/00—Component parts such as frames, beds, carriages, headstocks
- B24B41/06—Work supports, e.g. adjustable steadies
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B24—GRINDING; POLISHING
- B24B—MACHINES, DEVICES, OR PROCESSES FOR GRINDING OR POLISHING; DRESSING OR CONDITIONING OF ABRADING SURFACES; FEEDING OF GRINDING, POLISHING, OR LAPPING AGENTS
- B24B49/00—Measuring or gauging equipment for controlling the feed movement of the grinding tool or work; Arrangements of indicating or measuring equipment, e.g. for indicating the start of the grinding operation
- B24B49/006—Measuring or gauging equipment for controlling the feed movement of the grinding tool or work; Arrangements of indicating or measuring equipment, e.g. for indicating the start of the grinding operation taking regard of the speed
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B24—GRINDING; POLISHING
- B24B—MACHINES, DEVICES, OR PROCESSES FOR GRINDING OR POLISHING; DRESSING OR CONDITIONING OF ABRADING SURFACES; FEEDING OF GRINDING, POLISHING, OR LAPPING AGENTS
- B24B49/00—Measuring or gauging equipment for controlling the feed movement of the grinding tool or work; Arrangements of indicating or measuring equipment, e.g. for indicating the start of the grinding operation
- B24B49/10—Measuring or gauging equipment for controlling the feed movement of the grinding tool or work; Arrangements of indicating or measuring equipment, e.g. for indicating the start of the grinding operation involving electrical means
Abstract
The invention discloses a device integrating multi-channel aperture detection and nanopore preparation and a use method thereof. Wherein, the clamp holder, the linear bearing, the carrying disc, the ball bearing and the motor form a polishing part; the conductive probe, the man-machine interaction interface and the circuit form a detection control part; the holder, the linear bearing, the conductive probe, the carrier disc, the ball bearing, the motor, the man-machine interaction interface and the circuit are simultaneously arranged in the host shell to form the device integrating nano-pore preparation and nano-pore aperture detection. The invention realizes the real-time detection of the multi-channel aperture and the controllable preparation of the nano-holes, and has wide application prospect in the research of scientific problems in the channels under the nano scale.
Description
Technical Field
The invention relates to the field of nanopore preparation and nanopore aperture detection, in particular to a device integrating multichannel aperture detection and nanopore preparation.
Background
The glass nano pore canal is prepared by sealing the tip of a microfilament such as gold or platinum which is sharpened electrochemically at the end of a capillary. The capillary tip is polished manually using a high input impedance Metal Oxide Semiconductor Field Effect Transistor (MOSFET) based circuit to monitor the radius of the gold or platinum or other microwire tip exposed at the capillary tip. Based on the numerical analysis of the impedance of the polishing circuit, the MOSFET circuit is properly biased, the nano pore canal with the radius as small as 10nm can be repeatedly manufactured, and the size of the nano pore can be determined according to the current of the tip of the microfilament such as gold or platinum. However, currently, during manual grinding of glass nanopores, researchers receive a stop signal of the manual grinding alarm device and react longer than a machine, thereby causing excessive grinding; on the other hand, in the polishing process, the pore size of the nano-pore cannot be detected in real time, and a researcher needs to constantly observe the pore size through a microscope in the polishing process, so that the prepared pore size of the nano-pore has great contingency. Therefore, the existing glass nano pore canal preparation has the problems of low efficiency, low success rate and incapability of real-time pore diameter detection. Has wide application prospect in the research of scientific problems in the channel under the nanometer scale.
Disclosure of Invention
The invention aims to solve the problems and provide a device integrating multi-channel aperture detection and nanopore preparation, which can simultaneously realize nanopore preparation and real-time detection of the nanopore aperture and has wide application prospects in scientific problem research in channels under nanoscale.
In order to achieve the above purpose, the present invention adopts the following technical scheme.
The device integrating the multi-channel aperture detection and the nano-pore preparation comprises a host shell, wherein a circuit unit is arranged in the host shell, and a man-machine interaction interface is arranged on the front surface of the shell; the shell is provided with a support frame which comprises a vertical column and a horizontal plate; wherein the horizontal plate is positioned above the shell and is parallel to the upper surface of the shell;
the carrying disc is arranged at the middle part of the upper surface of the shell and right below the horizontal plate of the supporting frame; the carrying disc is internally provided with a grinding tool and a KCl solution with the concentration of 0.02M; the grinding tool can rotate relative to the horizontal plate;
the bottom of the center of the horizontal plate is provided with a conductive probe downwards, and the tail end of the conductive probe is immersed in 0.02M KCl solution of the carrier plate;
the horizontal plate is also provided with at least one perforation, and the perforation is used for being embedded into a linear bearing, and the linear bearing is inserted into the clamp holder; the capillary tube seal sharpening metal wire is inserted into the clamp holder, and the top end of the capillary tube seal sharpening metal wire is connected with the signal detection circuit; the capillary seal sharpening wire ends are immersed in a conductive solution (e.g., 0.02M KCl solution) of the carrier plate and are in contact with the carrier plate grinder.
The overall resistance of the capillary seal sharpening wire and the conductive solution is the sharpening resistance. In the polishing process, the aperture of the exposed tip end of the capillary seal sharpening metal wire is continuously increased, and the polishing resistance is continuously decreased.
Further, the abrasive article comprises sandpaper or a grinding wheel.
Further, the grinding tool rotates relative to the horizontal plate, and the carrier plate rotates through the driving unit through the synchronous rotation connection relation of the grinding tool and the carrier plate.
Further, the driving unit is a motor, the motor is arranged in the shell, and an output shaft of the motor and the carrier disc form a synchronous rotary connection relationship.
Further, the conductive solution is used for conducting the capillary seal sharpening wire and the conductive probe.
Further, the device also comprises a linear bearing, the linear bearing is embedded into the horizontal plate through hole, and the clamp holder penetrates through the linear bearing from top to bottom.
Further, the vertical column of the support frame is arranged on the upper surface of the shell near the edge.
Further, the man-machine interaction interface hardware part comprises an LCD display screen and a touch screen, and is provided with a graphical user interface on the basis.
Further, the circuit comprises a singlechip minimum system, a signal amplifying circuit, an analog-to-digital conversion circuit, a motor driving circuit, an upper computer communication circuit and a DC-DC conversion circuit.
Further, the man-machine interaction interface and the motor are used as a controller by a singlechip in the circuit.
Furthermore, multichannel signal acquisition is realized through a singlechip in the circuit, so that simultaneous preparation of multiple nanopores is realized.
Further, the graphical user interface comprises an electrical signal display, an aperture size display, a polishing time display, a polishing speed regulation and control, a switching function and a task completion prompt.
The invention also provides a using method of the device, and the rotating speed and the preparation time of the motor are controlled through a human-computer interaction interface, so that the preparation speed of the nano holes is controlled. The specific process comprises the steps of clamping a capillary tube seal sharpening metal wire in a clamp holder and automatically moving downwards in a direction perpendicular to a nanopore preparation plane; simultaneously, the bottom carrying disc drives the grinding tool to grind the tip of the capillary seal sharpening wire; when the tip of the capillary tube seal sharpening metal wire is exposed, the polishing resistance changes, signals are amplified and converted into digital signals through a circuit, and data are displayed in real time through a man-machine interaction interface.
The device integrating multi-channel aperture detection and nanopore preparation has the following positive effects:
can realize the preparation of the nano-pores, and detect and control the size of the prepared nano-pore diameter; meanwhile, the preparation of a plurality of glass nanopores and the detection of electric signals can be realized; the method helps scientific researchers to improve the preparation efficiency of the nano-pores and simultaneously can acquire related data in the preparation process, so that the method has wide application prospect in the research of scientific problems in channels under nano scale.
Drawings
The structure of the present invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic structural diagram of a device integrating multi-channel aperture detection and nanopore preparation according to the present invention
FIG. 2 is an exploded view of the device of the present invention integrating multi-channel aperture detection and nanopore preparation
FIG. 3 is a front view of the device integrating multi-channel aperture detection and nanopore preparation
FIG. 4 is a side view of the device integrating multi-channel aperture detection and nanopore preparation of the present invention
FIG. 5 is a top view of the device integrating multi-channel aperture detection and nanopore preparation
The reference numerals of the drawings are respectively: 1. a holder; 2. a linear bearing; 3. a conductive probe; 4. a carrier plate; 5. a ball bearing; 6. a motor; 7. a support frame; 8. a host housing; 9. a human-computer interaction interface; 10. a circuit;
Detailed Description
The following describes specific embodiments of a device integrating nanopore preparation and nanopore aperture detection with reference to the drawings, but it should be noted that the implementation of the present invention is not limited to the following embodiments.
The device integrating multi-channel aperture detection and nanopore preparation into a whole, referring to fig. 1-5, comprises a clamp holder 1, a linear bearing 2, a conductive probe 3, a carrier disc 4, a ball bearing 5, a motor 6, a support frame 7, a host shell 8, a man-machine interaction interface 9 and a circuit 10. Wherein, the clamp holder 1, the linear bearing 2, the carrying disc 4, the ball bearing 5 and the motor 6 form a polishing part; the conductive probe 3, the man-machine interaction interface 9 and the circuit 10 form a detection control part; the clamp holder 1, the linear bearing 2, the conductive probe 3, the carrying disc 4, the ball bearing 5, the motor 6, the man-machine interaction interface 9 and the circuit 10 are arranged in the host shell 8 to form a device integrating multi-channel aperture detection and nanopore preparation.
The host shell 8 on be equipped with support frame 7, support frame 7 include vertical post and horizontal plate two parts. The vertical column is vertically arranged on one side of the upper surface of the main machine shell, and the horizontal plate extends from the upper end of the vertical column to the upper side of the middle part of the main machine shell 8 and is parallel to the upper surface of the main machine shell 8. The carrying disc 4 is arranged at the middle part of the upper surface of the host shell 8, and is arranged right below the horizontal plate of the supporting frame 7.
The center of the bottom of the carrying disc 4 is fixed with the output shaft of the motor 6 to form a synchronous rotary connection relationship. A plurality of ball bearings 5 are also arranged between the bottom of the carrier disc 4 and the upper surface of the host casing 7, so that the carrier disc 4 rotates more stably.
A sanding material, in this embodiment sandpaper, is secured within the carrier disc 4. The carrier tray 4 is also provided with an electroconductive solution. In this example, a KCl solution was used at a concentration of 0.02M.
The center of the bottom of the horizontal plate of the supporting frame 7 is downwards provided with a conductive probe 3, and the tail end of the conductive probe is immersed in KCl solution with the concentration of 0.02M in the carrier plate 4. The horizontal plate of the support frame 7 is also provided with a plurality of perforations, and the linear bearings 2 are embedded in the perforations, the linear bearings are used for being inserted into the clamp holders 1, and the clamp holders 1 penetrate through the linear bearings 2 from top to bottom. The linear bearing 2 prevents the clamp 1 from shaking, and is smoother when moving up and down. In this embodiment, the holders 1 are provided in four numbers, respectively provided at four corners of the horizontal plate of the support frame 7.
The circuit 10 comprises a singlechip minimum system, a signal amplifying circuit, an analog-to-digital conversion circuit, a motor driving circuit, an upper computer communication circuit and a DC-DC conversion circuit, and is mainly used for signal acquisition processing, motor driving, power management and upper computer data transmission. The microcontroller simultaneously controls the man-machine interaction interface and the detection preparation circuit.
The man-machine interaction interface comprises an LCD display screen, a graphical user interface and a touch screen. The system is used for controlling the polishing speed and the switch in the polishing process, and displaying polishing loop data acquired and processed by the microcontroller and the detection circuit in the polishing process, so that a user can observe experimental data and aperture change in the polishing process in real time, and the data can be uploaded to the upper computer in real time.
In the specific implementation process, after the sand paper is fixed on the carrying disc 4, adding 0.02M KCl solution; and then the capillary tube seal sharpening metal wire is put into the clamp holder 1 and fixedly inserted into the linear bearing 2, and the upper end of the capillary tube seal sharpening metal wire is connected into the signal detection circuit through a lead. The clamp holder 1 automatically moves downwards continuously due to the gravity; turning on a polishing switch, and driving the abrasive paper by the carrier disc 4 to continuously polish the capillary tube sealed with the sharpening metal wire; when the capillary tube sealed sharpening metal wire exposes a proper nano aperture, polishing is stopped, the capillary tube sealed with the sharpening metal wire is taken out, and the capillary tube sealed with the sharpening metal wire is corroded by an acid solution, so that the glass nano aperture can be obtained.
The working principle of the device integrating the multi-channel aperture detection and the nano-pore preparation instrument into a whole is as follows: the capillary seal sharpening metal is put into a clamp holder and fixedly inserted into a linear bearing, the clamp holder is continuously worn out by the action of gravity,will automatically move downwards continuously; the motor drives the carrier disc fixed with sand paper and added with 0.02M KCl solution to rotate to form polishing action; during the polishing process, when the capillary seal sharpening wire is exposed to the proper nanoaperture, the polishing resistance can be reduced from 10 18 Down to the order of 10 9 The magnitude of the electrical signal is mutated. Through the setting of a user, the microcontroller and the detection preparation circuit can detect the mutation of the electric signal, collect and calculate the signal and detect whether the aperture meets the requirement of the user. In the polishing process, if the aperture requirement set by the user is not met, continuing polishing until the requirement of the user is met, stopping polishing, and indicating which capillary tube sealed with the sharpened wire is polished.
The device integrating multi-channel aperture detection and nanopore preparation can realize nanopore preparation, and detect and control the size of the prepared nanopore; meanwhile, the preparation of a plurality of glass nanopores and the detection of electric signals can be realized; the method helps scientific researchers to improve the preparation efficiency of the nano holes and simultaneously can acquire related data in the preparation process, so that the method has wide application prospects in the research of scientific problems in channels under nano scale.
Claims (10)
1. The utility model provides a collect multichannel aperture detection and nanopore preparation in device of an organic whole which characterized in that: the device comprises a host shell, wherein a circuit unit is arranged in the host shell, and a man-machine interaction interface is arranged on the front surface of the shell; the shell is provided with a support frame which comprises a vertical column and a horizontal plate; wherein the horizontal plate is positioned above the shell and is parallel to the upper surface of the shell;
the carrying disc is arranged at the middle part of the upper surface of the shell and right below the horizontal plate of the supporting frame; and the carrying disc is internally provided with a grinding tool and a conductive solution; the grinding tool can rotate relative to the horizontal plate;
the bottom of the center of the horizontal plate is provided with a conductive probe downwards, the tail end of the conductive probe is immersed in a conductive solution of the carrier disc, and the upper end of the conductive probe is connected into a signal detection circuit;
the horizontal plate is also provided with at least one perforation, the perforation is used for being embedded into a linear bearing, and the linear bearing is inserted into the clamp holder so as to keep the clamp holder stable in the polishing process; the capillary tube seal sharpening metal wire is inserted into the clamp holder, and the top end of the capillary tube seal sharpening metal wire is connected with the signal detection circuit; the end of the capillary seal sharpening wire is immersed by gravity in the conductive solution of the carrier plate and is in contact with the carrier plate grinder.
2. The device integrating multi-channel aperture detection and nanopore preparation as claimed in claim 1, wherein: the grinding tool is sand paper or grinding wheel.
3. The device integrating multi-channel aperture detection and nanopore preparation as claimed in claim 1, wherein: the grinding tool rotates relative to the horizontal plate, and the grinding tool and the carrier disc are in synchronous rotation connection, and the carrier disc rotates through the driving unit.
4. A device for integrating multi-channel pore size detection and nanopore preparation as claimed in claim 3, wherein: the driving unit is a motor, the motor is arranged in the shell, and an output shaft of the motor and the carrier disc form a synchronous rotary connection relationship.
5. The device integrating multi-channel aperture detection and nanopore preparation as claimed in claim 1, wherein: the clamp is characterized by further comprising a linear bearing, wherein the linear bearing is embedded into the horizontal plate through hole, and the clamp holder penetrates through the linear bearing from top to bottom.
6. The device integrating multi-channel aperture detection and nanopore preparation as claimed in claim 1, wherein: the vertical column of the support frame is arranged on the upper surface of the shell and is close to the edge.
7. The device integrating multi-channel aperture detection and nanopore preparation as claimed in claim 1, wherein: the holder includes a collet and a hollow stem for holding the capillary seal sharpening wire.
8. The device integrating multi-channel aperture detection and nanopore preparation as claimed in claim 1, wherein: the man-machine interaction interface hardware comprises an LCD display screen and a touch screen, and a graphical user interface is carried on the basis of the hardware.
9. The device integrating multi-channel pore size detection and nanopore preparation as claimed in claim 8, wherein: the graphical user interface comprises an electrical signal display, an aperture size display, a polishing time display, a polishing speed regulation and control, a switching function and a task completion prompt.
10. A method of using a device according to any one of claims 1 to 9, wherein: controlling the rotating speed and the preparation time of the motor through a human-computer interaction interface, thereby controlling the preparation speed of the nano-pores; the capillary tube sealing sharpening metal wire is clamped in the clamp and automatically moves downwards in the direction perpendicular to the nano-pore preparation plane, and the tail end of the capillary tube sealing sharpening metal wire is always immersed in the conductive solution and is contacted with the grinding tool; simultaneously, the bottom carrying disc drives the grinding tool to grind the tip of the capillary seal sharpening wire; when the tip of the capillary tube seal sharpening metal wire is exposed, the polishing resistance changes, a real-time electric signal is obtained through a signal detection circuit, the signal is amplified and converted into a digital signal by the circuit, and the data is displayed in real time through a human-computer interaction interface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211135546.9A CN115464511B (en) | 2022-09-19 | 2022-09-19 | Device integrating multi-channel aperture detection and nanopore preparation and application method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211135546.9A CN115464511B (en) | 2022-09-19 | 2022-09-19 | Device integrating multi-channel aperture detection and nanopore preparation and application method thereof |
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| Publication Number | Publication Date |
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| CN115464511A CN115464511A (en) | 2022-12-13 |
| CN115464511B true CN115464511B (en) | 2023-08-01 |
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| CN202211135546.9A Active CN115464511B (en) | 2022-09-19 | 2022-09-19 | Device integrating multi-channel aperture detection and nanopore preparation and application method thereof |
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| CN114290223A (en) * | 2021-12-14 | 2022-04-08 | 朗峰新材料启东有限公司 | A burnishing and polishing device for production of nanocrystalline strip |
| CN114789378A (en) * | 2022-05-31 | 2022-07-26 | 福建福晶科技股份有限公司 | Polishing device and method with ultrahigh damage threshold on surface |
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| US7465661B2 (en) * | 2003-05-28 | 2008-12-16 | The United States Of America As Represented By The Secretary Of The Navy | High aspect ratio microelectrode arrays |
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| CN1708377A (en) * | 2002-10-28 | 2005-12-14 | 卡伯特微电子公司 | Transparent microporous materials for CMP |
| CN201579692U (en) * | 2009-12-30 | 2010-09-15 | 东南大学 | Polishing device of electrochemical electrode |
| CN102320564A (en) * | 2011-09-02 | 2012-01-18 | 厦门大学 | Nanopore preparation method based on tungsten needle tip and thick-wall glass tube |
| CN103551926A (en) * | 2013-11-11 | 2014-02-05 | 广东工业大学 | Device for polishing micropores through electrophoresis auxiliary micro-ultrasonic or micro-rotating ultrasonic and processing method |
| CN105033838A (en) * | 2015-07-27 | 2015-11-11 | 郑州大学 | Forming method for metal surface micro-nano holes through mechanical lapping and lapping and polishing machine |
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| CN115464511A (en) | 2022-12-13 |
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