CN115462444A - Clostridium butyricum animal feed and application thereof - Google Patents
Clostridium butyricum animal feed and application thereof Download PDFInfo
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
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Abstract
The invention belongs to the technical field of animal feed, and particularly provides clostridium butyricum animal feed, which comprises the following components in percentage by weight: 10-15 parts of clostridium butyricum, 55-65 parts of corn, 2-4 parts of wheat bran, 20-30 parts of soybean meal, 2-4 parts of rapeseed meal, 1-2 parts of calcium hydrophosphate, 1-2 parts of amino acid composition, 1-3 parts of stone powder, 0.3-0.5 part of flavoring agent, 1-3 parts of yeast powder, 1-2 parts of compound vitamin and 1-2 parts of trace elements. The clostridium butyricum animal feed provided by the invention can improve the immunity of organisms, effectively enhance the resistance of chicks to avian infectious bronchitis virus infection, reduce morbidity and detoxification, can effectively control the diffusion of viruses in a feeding farm, and has wide application prospects.
Description
Technical Field
The invention belongs to the technical field of animal feed, and particularly relates to clostridium butyricum animal feed and application thereof.
Background
Avian Infectious Bronchitis (IB) is a highly Infectious viral disease caused by Avian Infectious Bronchitis Virus (IBV). IBV is a coronavirus, has high pathogenicity and contact infectivity, has infectivity for chickens of different ages in days, has the pathogenicity rate as high as 100 percent, has the death rate of infected chicks of 25 to 30 percent, reduces the quality of broilers of infected chicken groups, reduces the egg yield of laying hens, and brings great economic loss to the poultry industry.
At present, attenuated live vaccines are widely used for preventing and controlling avian infectious bronchitis. Inactivated vaccines are generally used in combination with live attenuated vaccines, either alone. However, there are many serotypes of the virus, and the antigenic diversity of the viruses prevents effective cross protection between different strains of the virus. The avian infectious bronchitis virus strains widely prevalent in China are mainly QX genotypes, and the prevention and control means of the strains comprise a small amount of vaccines and the like, so that the vaccine prevention and control effect is poor.
The intestinal microbiota plays an important role in the normal metabolism of the host and in maintaining the physiological health of the body. In recent years, the intestinal microbiome of livestock and poultry is concerned by the poultry industry, and the microbiology is utilized to prepare the feed with a new formula which has the effects of improving the immunity of the organism so as to enhance the antiviral infection capability of animals and improving the production performance of animals, so that the feed is beneficial to the healthy development of the livestock and poultry breeding industry, reduces the risk of food-borne diseases spreading to human beings, and is beneficial to public health and safety.
Disclosure of Invention
The invention aims to solve the problems of few prevention and control means of avian infectious bronchitis viruses and poor vaccine prevention and control effect in the prior art.
To this end, the present invention provides a clostridium butyricum animal feed comprising: 10-15 parts of clostridium butyricum, 55-65 parts of corn, 2-4 parts of wheat bran, 20-30 parts of soybean meal, 2-4 parts of rapeseed meal, 1-2 parts of calcium hydrophosphate, 1-2 parts of amino acid composition, 1-3 parts of stone powder, 0.3-0.5 part of flavoring agent, 1-3 parts of yeast powder, 1-2 parts of composite vitamin and 1-2 parts of trace elements.
Specifically, the bacterial activity of the Clostridium butyricum is l0 6 -10 7 CFU/μl。
Specifically, the amino acid composition comprises, by mass, 47:21:10 lysine, methionine and threonine.
Specifically, the flavoring agent is salt.
Specifically, the above compound vitamins include 0.15-0.25% of vitamin A, 0.03-0.05% of vitamin D, 0.25-0.35% of vitamin E, K3-85% of vitamin, 0.35-0.50% of vitamin, 1.1-1.5% of vitamin B2, B6 0.7-0.9% of vitamin, 0.0035-0.0045% of vitamin B12, 7-9.1% of nicotinic acid, 0.15-0.23% of folic acid, and 0.007-0.009% of biotin.
Specifically, the microelements comprise 25-30% of iron, 2-2.5% of copper, 30-40% of manganese, 25-35% of zinc, 0.15-0.25% of iodine, and 0.075-0.1% of selenium.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the clostridium butyricum animal feed provided by the invention can improve the immunity of organisms, effectively enhance the resistance of chicks to avian infectious bronchitis virus infection, reduce morbidity and toxin expulsion, effectively control the diffusion of viruses in a feeding farm, and has wide application prospects.
The present invention will be described in further detail below with reference to the accompanying drawings.
Drawings
FIG. 1 is a graph showing the results of the disease incidence of 21 st day chickens challenged with the toxin of example 4 of the present invention.
FIG. 2 is a graph showing the results of the detoxification rate of 21 day chickens challenged with the toxin of example 4 of the present invention.
FIG. 3 is a graph of the viral load of day 3, 5, 7 tubes challenged in example 4 of the invention.
FIG. 4 shows the renal viral loads on days 3, 5 and 7 of the challenge in example 4 of the present invention.
FIG. 5 is a photograph of pathological sections of trachea and kidney at day 7 of the challenge in example 4 of the present invention.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the following embodiments, and it should be apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Although representative embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that various modifications and changes may be made thereto without departing from the scope of the invention. Therefore, the scope of the present invention should not be limited to the embodiments, but should be defined by the appended claims and equivalents thereof.
The effect of the clostridium butyricum animal feed of the present invention is studied by the following specific examples.
Example 1:
the embodiment provides a clostridium butyricum animal feed which comprises 12 parts of clostridium butyricum, 55 parts of corn, 3 parts of wheat bran, 20 parts of soybean meal, 3 parts of rapeseed meal, 1.5 parts of amino acid composition, 1.5 parts of calcium hydrophosphate, 2 parts of stone powder, 0.3 part of salt, 2 parts of yeast powder, 1.5 parts of multivitamin and 1.5 parts of trace elements.
Wherein the bacterial activity of Clostridium butyricum is 10 7 CFU/. Mu.l; the amino acid composition comprises 47 mass ratio: 21: lysine, methionine and threonine of 10; the compound vitamins include vitamin A1000 IU, vitamin D200 IU, vitamin E1.5 mg, vitamin K3 mg, vitamin B1.96 mg, vitamin B2.76 mg, vitamin B6.92 g, vitamin B12.02 mg, nicotinic acid 39.2mg, folic acid 0.98mg and biotin 0.04mg; the microelements comprise iron 100mg, copper 8mg, manganese 120mg, zinc 100mg, iodine 0.7mg, and selenium 0.3mg.
Example 2:
the embodiment provides clostridium butyricum animal feed which comprises 10 parts of clostridium butyricum, 60 parts of corn, 2 parts of wheat bran, 25 parts of soybean meal, 2 parts of rapeseed meal, 1 part of amino acid composition, 1 part of calcium hydrophosphate, 1 part of stone powder, 0.3 part of salt, 1 part of yeast powder, 1 part of multivitamin and 1 part of trace elements.
Wherein the bacterial activity of Clostridium butyricum is 10 7 CFU/. Mu.l; the amino acid composition comprises 47 mass percent: 21:10 lysine, methionine and threonine; the compound vitamins include vitamin A1000 IU, vitamin D200 IU, vitamin E1.5 mg, vitamin K3 mg, vitamin B1.96 mg, vitamin B2.76 mg, vitamin B6.92 g, vitamin B12.02 mg, nicotinic acid 39.2mg, folic acid 0.98mg, and biotin 0.04mg; the microelements comprise iron 100mg, copper 8mg, manganese 120mg, zinc 100mg, iodine 0.7mg, and selenium 0.3mg.
Example 3:
the embodiment provides a clostridium butyricum animal feed which comprises 15 parts of clostridium butyricum, 65 parts of corn, 4 parts of wheat bran, 30 parts of soybean meal, 4 parts of rapeseed meal, 2 parts of an amino acid composition, 2 parts of calcium hydrophosphate, 3 parts of stone powder, 0.5 part of salt, 3 parts of yeast powder, 2 parts of multivitamin and 2 parts of trace elements.
Wherein the bacterial activity of Clostridium butyricum is 10 6 CFU/. Mu.l; the amino acid composition comprises 47 mass ratio: 21:10 lysine, methionine and threonine; the compound vitamins include vitamin A1000 IU, vitamin D200 IU, vitamin E1.5 mg, vitamin K3 mg, vitamin B1.96 mg, vitamin B2.76 mg, vitamin B6.92 g, vitamin B12.02 mg, nicotinic acid 39.2mg, folic acid 0.98mg and biotin 0.04mg; the microelements comprise iron 100mg, copper 8mg, manganese 120mg, zinc 100mg, iodine 0.7mg, and selenium 0.3mg.
Example 4:
this example studies the effect of clostridium butyricum animal feed in chick feeding. The commercial chick feed used in this example included 62% corn, 4% wheat bran, 28.2% soybean meal, 1.3% calcium hydrogen phosphate, 1.2% stone powder, 0.3% salt, 1% amino acids, 1% vitamins, 1% trace elements.
Selecting 1-day-old SPF chicks, and randomly and equally dividing the SPF chicks into a positive control group, a negative control group, a formula feed group, a control group 1 and a control group 2.
Positive control group: feeding commercial chicken feed;
negative control group: feeding commercial chicken feed;
the formula experimental group is as follows: feeding the clostridium butyricum animal feed provided in example 1;
formulation control group 1: feeding commercial chick feed added with clostridium butyricum with the bacterial activity of 10 7 CFU/. Mu.l, the addition amount in the feed is the same as that of the clostridium butyricum in the example 1;
formulation control 2: the feed is fed to the feed prepared by the formula except the clostridium butyricum in the example 1.
All the groups are raised in an isolator, 10 chicks are raised per square meter, the temperature of the isolator is controlled to be about 30 ℃ in 21 days of the test period, natural illumination and daylight lamp illumination are combined, and the chicks can eat and drink water freely.
When the chickens are 10 days old, the QX type IBV strains are used for eye-drop and nose-drop poison attacking on the chickens of a positive control group, a formula feed group, a formula control group 1 and a formula control group 2. The dosage is 10 5 EID 50 After challenge, the status of the chickens was observed daily and the incidence of the chickens was recorded as shown in fig. 1. The pathological changes of various organs are examined 21 days after the toxin is attacked, an anal swab is taken, and the toxin expelling rate is detected by RT-PCR, and the result is shown in figure 2. Tracheal and renal tissues were harvested on days 3, 5, and 7 after challenge, and viral load was measured, as shown in fig. 3 and 4. Pathological sections of the trachea and the kidney are taken on the 7 th day of toxin attack for examination, the pathological sections are magnified by 200 times, as shown in figure 5, the positive control group and the formula control group 2 have lesions such as obvious organ cilia separation, cell necrosis and the like, and the formula experimental group, the formula control group 1 and the negative control group have no obvious lesions.
The above examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention, which is intended to be covered by the claims and any design similar or equivalent to the scope of the invention.
Claims (8)
1. A Clostridium butyricum animal feed comprising: 10-15 parts of clostridium butyricum, 55-65 parts of corn, 2-4 parts of wheat bran, 20-30 parts of soybean meal, 2-4 parts of rapeseed meal, 1-2 parts of calcium hydrophosphate, 1-2 parts of amino acid composition, 1-3 parts of stone powder, 0.3-0.5 part of flavoring agent, 1-3 parts of yeast powder, 1-2 parts of compound vitamin and 1-2 parts of trace elements.
2. The clostridium butyricum animal feed of claim 1, wherein: the bacterial activity of the clostridium butyricum is l0 6 -10 7 CFU/μl。
3. The clostridium butyricum animal feed of claim 1, wherein: the amino acid composition comprises 47 mass ratio: 21:10 lysine, methionine and threonine.
4. The clostridium butyricum animal feed of claim 1, wherein: the feed additive is choline chloride.
5. The clostridium butyricum animal feed of claim 1, wherein: the flavoring agent is salt.
6. Clostridium butyricum animal feed according to claim 1, wherein: the compound vitamin comprises 0.15-0.25% of vitamin A, 0.03-0.05% of vitamin D, 0.25-0.35% of vitamin E, K3 75-85% of vitamin, 0.35-0.50% of vitamin, 1.1-1.5% of vitamin B2, B6 0.7-0.9% of vitamin, 0.0035-0.0045% of vitamin B12, 7-9.1% of nicotinic acid, 0.15-0.23% of folic acid and 0.007-0.009% of biotin.
7. The clostridium butyricum animal feed of claim 1, wherein: the microelements comprise 25-30% of ferrum, 2-2.5% of copper, 30-40% of manganese, 25-35% of zinc, 0.15-0.25% of iodine, and 0.075-0.1% of selenium.
8. Use of a clostridium butyricum animal feed according to any one of claims 1 to 7 for combating avian infectious bronchovirus.
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| CN202211038976.9A CN115462444A (en) | 2022-08-29 | 2022-08-29 | Clostridium butyricum animal feed and application thereof |
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| CN202211038976.9A CN115462444A (en) | 2022-08-29 | 2022-08-29 | Clostridium butyricum animal feed and application thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1324618A (en) * | 1970-12-29 | 1973-07-25 | Shionogi & Co | Infectious coryza infectious bronchitis and newcastle disease vaccines |
| CN102461732A (en) * | 2010-11-05 | 2012-05-23 | 天津瑞普生物技术股份有限公司 | Composition containing butyric acid bacteria |
| CN107712392A (en) * | 2017-09-30 | 2018-02-23 | 安徽麒麟正大饲料有限公司 | 4% premix for preventing and treating infectious bronchitis of chicken and preparation method thereof |
| CN109566900A (en) * | 2018-12-07 | 2019-04-05 | 成都铁骑力士饲料有限公司 | A kind of special compound feed and preparation method thereof for rock hawk chicken brood time |
| CN114431360A (en) * | 2021-12-29 | 2022-05-06 | 广州无两生物科技有限公司 | Feed formula for black soldier fly powder chicks and application of feed formula to resisting avian infectious bronchitis viruses |
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- 2022-08-29 CN CN202211038976.9A patent/CN115462444A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1324618A (en) * | 1970-12-29 | 1973-07-25 | Shionogi & Co | Infectious coryza infectious bronchitis and newcastle disease vaccines |
| CN102461732A (en) * | 2010-11-05 | 2012-05-23 | 天津瑞普生物技术股份有限公司 | Composition containing butyric acid bacteria |
| CN107712392A (en) * | 2017-09-30 | 2018-02-23 | 安徽麒麟正大饲料有限公司 | 4% premix for preventing and treating infectious bronchitis of chicken and preparation method thereof |
| CN109566900A (en) * | 2018-12-07 | 2019-04-05 | 成都铁骑力士饲料有限公司 | A kind of special compound feed and preparation method thereof for rock hawk chicken brood time |
| CN114431360A (en) * | 2021-12-29 | 2022-05-06 | 广州无两生物科技有限公司 | Feed formula for black soldier fly powder chicks and application of feed formula to resisting avian infectious bronchitis viruses |
Non-Patent Citations (1)
| Title |
|---|
| 祖立闯 等: "丁酸梭菌的生物学功能及其在养猪生产中的研究与应用进展", 《养猪》, no. 1, pages 17 - 20 * |
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