CN115452905A - Electrochemical impedance meter based on smart phone and use method - Google Patents
Electrochemical impedance meter based on smart phone and use method Download PDFInfo
- Publication number
- CN115452905A CN115452905A CN202211373912.4A CN202211373912A CN115452905A CN 115452905 A CN115452905 A CN 115452905A CN 202211373912 A CN202211373912 A CN 202211373912A CN 115452905 A CN115452905 A CN 115452905A
- Authority
- CN
- China
- Prior art keywords
- electrochemical impedance
- impedance
- module
- channel
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000001514 detection method Methods 0.000 claims abstract description 76
- 238000004891 communication Methods 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims abstract description 11
- 239000004973 liquid crystal related substance Substances 0.000 claims abstract description 6
- 239000011159 matrix material Substances 0.000 claims abstract description 6
- 230000002093 peripheral effect Effects 0.000 claims abstract description 6
- 229940088597 hormone Drugs 0.000 claims description 28
- 239000005556 hormone Substances 0.000 claims description 28
- 239000000427 antigen Substances 0.000 claims description 9
- 102000036639 antigens Human genes 0.000 claims description 9
- 108091007433 antigens Proteins 0.000 claims description 9
- 239000002114 nanocomposite Substances 0.000 claims description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 239000000523 sample Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000003113 dilution method Methods 0.000 claims description 3
- 239000012488 sample solution Substances 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 239000003990 capacitor Substances 0.000 claims description 2
- 238000002847 impedance measurement Methods 0.000 description 12
- 238000005259 measurement Methods 0.000 description 9
- 238000013461 design Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000003321 amplification Effects 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000002593 electrical impedance tomography Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 150000001721 carbon Chemical class 0.000 description 1
- 238000013523 data management Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005476 size effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04B—TRANSMISSION
- H04B5/00—Near-field transmission systems, e.g. inductive or capacitive transmission systems
- H04B5/70—Near-field transmission systems, e.g. inductive or capacitive transmission systems specially adapted for specific purposes
- H04B5/73—Near-field transmission systems, e.g. inductive or capacitive transmission systems specially adapted for specific purposes for taking measurements, e.g. using sensing coils
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/72—Mobile telephones; Cordless telephones, i.e. devices for establishing wireless links to base stations without route selection
- H04M1/724—User interfaces specially adapted for cordless or mobile telephones
- H04M1/72403—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality
- H04M1/72409—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality by interfacing with external accessories
- H04M1/72412—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality by interfacing with external accessories using two-way short-range wireless interfaces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02D—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN INFORMATION AND COMMUNICATION TECHNOLOGIES [ICT], I.E. INFORMATION AND COMMUNICATION TECHNOLOGIES AIMING AT THE REDUCTION OF THEIR OWN ENERGY USE
- Y02D30/00—Reducing energy consumption in communication networks
- Y02D30/70—Reducing energy consumption in communication networks in wireless communication networks
Landscapes
- Engineering & Computer Science (AREA)
- Computer Networks & Wireless Communication (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Signal Processing (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Human Computer Interaction (AREA)
- Molecular Biology (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
The invention relates to an electrochemical impedance meter based on a smart phone and a using method thereof, and the electrochemical impedance meter specifically comprises a multi-channel electrochemical impedance sensor, an electrochemical impedance detection module and an Android smart phone; the multi-channel electrochemical impedance sensor comprises a plurality of channel working electrodes, a pair of reference electrodes and a pair of counter electrodes; the electrochemical impedance detection module comprises an STM32 main control module, a plurality of single-channel electrochemical impedance modules in communication connection with the STM32 main control module, a Bluetooth transmission module, a liquid crystal display module, a matrix keyboard module and a power supply module; the electrochemical impedance module is in communication connection with an electrode channel of the electrochemical impedance sensor and comprises an AD5933 chip, a low impedance amplifying circuit and a peripheral circuit; the STM32 main control module drives and controls the electrochemical impedance module, and transmits detection data to the Android smart phone through the Bluetooth transmission module; the smart phone displays the detection result in a graph and numerical form and stores the data.
Description
Technical Field
The invention relates to an electrochemical impedance meter, in particular to an electrochemical impedance meter based on a smart phone and a using method thereof.
Background
With the continuous development of sensor technology, the electrochemical sensor is widely concerned by researchers, can be applied to the detection of environmental hormones, utilizes the nanocomposite and the electroactive substance to modify the electrochemical sensor to carry out impedance detection so as to achieve the purpose of detecting the environmental hormones with different concentrations, and becomes a novel detection method. Usually, impedance measurement is mainly realized by using a professional detection instrument, such as an electrochemical workstation Autolab, gamry and the like, but the instrument is expensive in price, large in size, inconvenient to carry, and difficult to meet the requirements of hormone field, rapid and high-sensitivity impedance detection.
Disclosure of Invention
The invention provides an electrochemical impedance meter based on a smart phone, which comprises a multi-channel electrochemical impedance sensor, an electrochemical impedance detection module and an Android smart phone, wherein the multi-channel electrochemical impedance sensor is connected with the electrochemical impedance detection module; the multi-channel electrochemical impedance sensor comprises a plurality of channel working electrodes, a pair of reference electrodes and a pair of counter electrodes; the electrochemical impedance detection module comprises an STM32 main control module, a plurality of single-channel electrochemical impedance modules in communication connection with the STM32 main control module, a Bluetooth transmission module, a liquid crystal display module, a matrix keyboard module and a power supply module; the electrochemical impedance module is in communication connection with an electrode channel of the electrochemical impedance sensor and comprises an AD5933 chip, a low impedance amplifying circuit and a peripheral circuit;
the STM32 main control module drives and controls the electrochemical impedance module, and transmits detection data to the Android smart phone through a Bluetooth transmission module; the Android smart phone displays the detection result in a graph and numerical form, and stores data.
The invention also provides a use method of the electrochemical impedance meter for detecting hormone based on the smart phone, which comprises the following steps: modifying a working electrode of the multi-channel electrochemical impedance sensor by the carbon-based nano composite material and the antibody of the object to be detected, and then repeatedly cleaning by ultrapure water; preparing a series of hormone standard antigen solutions by adopting a proportional dilution method; parameter setting is carried out on an electrochemical impedance meter, hormone standard antigen solutions with different concentrations are measured, corresponding impedance results are obtained, fitting is carried out according to a Randles circuit, and impedance values corresponding to hormones with different concentrations are respectively obtained according to the corresponding relation between the impedance values and impedance curves; and respectively taking hormones with different concentrations and corresponding impedance values obtained by detection of the hormones with different concentrations as horizontal and vertical coordinates, obtaining a fitting curve between the concentration of the hormones and the impedance by using a linear fitting method, and writing the obtained fitting curve into an STM32 main control code for subsequent detection. And dropwise adding a sample solution to be detected to the sample adding position of the multi-channel electrochemical impedance sensor by using a pipette, enabling the sample to be detected to reach the surface of the multi-channel working electrode through the siphonage effect of cellulose filter paper, standing for a preset time, inserting an electrode joint of the multi-channel electrochemical impedance sensor into a slot of an electrochemical impedance meter after an antigen and an antibody are fully reacted, carrying out impedance detection according to the detection method in the step, obtaining a corresponding impedance value according to a multi-channel detection result, and bringing the impedance value into an impedance standard curve equation so as to obtain the concentration of the object to be detected.
Preferably, the multi-channel working electrode of the multi-channel electrochemical impedance sensor is selected by rotating a switch to select different working electrode channels.
Preferably, the surface of the working electrode of the electrochemical impedance sensor is modified with the carbon-based nanocomposite material.
The invention has the beneficial effects that: the invention adopts a high-precision electrochemical impedance measurement method, and can realize impedance measurement of different modification layers, thereby realizing detection of measured objects with different concentrations. The multi-channel electrochemical impedance sensor comprises a plurality of working electrodes, a reference electrode and a counter electrode, can simultaneously carry out impedance detection on various different objects to be detected, and has high detection efficiency. The wireless Bluetooth module breaks the restriction of traditional wired communication, can realize wireless communication of data, and greatly expands the application range of equipment. The invention has the advantages of accurate detection, lower cost, simple and reasonable structure, small volume, portability and wide market prospect, and can be used at any time.
Drawings
FIG. 1 is a block diagram of the overall structural design of the present invention;
FIG. 2 is a circuit diagram of an electrochemical impedance module of the present invention;
FIG. 3 is a circuit diagram of an electrochemical impedance detection module according to the present invention;
FIG. 4 is a diagram illustrating the results of the impedance test according to the embodiment of the present invention.
Detailed Description
In the following description, for purposes of explanation and not limitation, specific details are set forth, such as particular circuit diagrams, in order to provide a thorough understanding of the embodiments of the invention. In other instances, detailed descriptions of well-known systems, devices, circuits, and methods are omitted so as not to obscure the description of the present invention with unnecessary detail.
The invention provides a multi-channel electrochemical impedance meter based on a smart phone, which comprises a multi-channel electrochemical impedance sensor, an electrochemical impedance detection module and an Android smart phone.
The electrochemical impedance detection module comprises an STM32 main control module, a plurality of single-channel electrochemical impedance modules, a Bluetooth transmission module, a liquid crystal display module, a matrix keyboard module and a power supply module.
As shown in fig. 1, the multi-channel electrochemical impedance sensor is connected with the electrochemical impedance module to convert a hormone concentration signal to be detected into an electrochemical impedance electrical signal, and a detection result of the electrochemical impedance detection module is transmitted to the Android smartphone through the wireless bluetooth module to store and display the electrochemical impedance detection signal. The invention solves the problem of lacking of portable impedance detection equipment for rapid hormone detection.
In the embodiment, the multi-channel electrochemical impedance sensor comprises a plurality of symmetrically arranged multi-channel n (n =6 to 12) working electrodes, a pair of reference electrodes and a pair of counter electrodes, wherein the multi-channel working electrodes can select different working electrode channels by using a rotary change-over switch; the electrochemical impedance sensor surface modified carbon-based nanocomposite is used for hormone detection, and the carbon-based nanocomposite forms a loose porous structure on the sensor surface to adsorb protein molecules and an electroactive substance amplification detection signal; the multi-channel electrochemical impedance sensor is connected with the electrochemical impedance detection module through the connecting component and can be used for simultaneously detecting multiple hormones; the nano material has excellent physical and chemical properties such as quantum size effect, surface effect and the like, so that the amplification of a detection signal can be realized, and the problems of poor result reproducibility, high false positive rate and false negative rate and the like of an immunoassay method can be solved.
The multi-channel electrochemical impedance detection module comprises an STM32 main control module, an electrochemical impedance module, a Bluetooth transmission module, a liquid crystal display module, a matrix keyboard module and a power supply module.
The electrochemical impedance detection module in the embodiment comprises six channels, the requirements of different detection precision ranges can be met, the wireless Bluetooth data transmission function can be realized, the real-time communication function with an Android smart phone is realized, the instant and rapid detection is realized, the detection result is displayed on an Android smart phone interface in a graph and numerical form in real time and is stored in a database inside the mobile phone, and the number of the channels of the electrochemical impedance detection module can be expanded according to the detection precision requirements of a multi-channel electrochemical impedance sensor.
And the STM32 main control module is used for generating an impedance detection control algorithm, driving the electrochemical impedance module and setting an initial parameter value of the electrochemical impedance module. The detection signal is subjected to signal amplification, filtering and other processing through the electrochemical impedance module and then applied to the electrode end of the electrochemical impedance sensor to detect the hormone concentration.
The power supply module is used for providing proper power supply voltage for the STM32 main control module, and the matrix keyboard module is used for inputting parameter values and the like.
Electrochemical impedance detection module: the high-precision impedance conversion chip AD5933 is adopted to realize impedance detection with different precisions; the electrochemical impedance module of each single channel is realized by an AD5933 chip, a low-impedance amplifying circuit and a peripheral circuit.
As shown in fig. 2, AD5933 internally contains a frequency generator and a 12-bit analog-to-digital converter ADC, with a conversion rate of bit 1MSPS. The impedance measurement can control an internal register to set the initial frequency, the frequency resolution and the number of scanning points through an STM32 chip, different output voltage ranges of a frequency generator are selected, an excitation signal generated by the frequency signal generator is applied to two ends of the impedance to be measured, high-speed sampling is carried out through an on-chip ADC (analog to digital converter), fourier variation DFT (discrete Fourier transform) is realized on sampling data through an on-chip DSP (digital signal processor), a measurement result is stored in a corresponding memory, and the impedance measurement is completed through calibration calculation.
The AD5933 chip has the limitations of high output impedance of a drive signal, weak drive capability of drive voltage, non-VDD/2 of direct current bias and the like, which affect the measurement precision, and the measurement precision needs to be improved by optimizing a peripheral circuit. The peripheral circuitry not only removes some of the limitations of the AD5933 chip itself, but also improves the accuracy and precision of the impedance measurements.
The low-impedance amplifying circuit is mainly used for assisting in measuring the resistor to be measured with a small impedance value. The high-precision operational amplifier OPA4364, the T-shaped feedback resistance network and the capacitor are designed to form an amplifying circuit for amplifying a detection signal so as to complete small-impedance resistance measurement.
OPA4364 is a high-performance CMOS operational amplifier with 7MHz bandwidth, 90dB CMRR and 5V/us slew rate. The design utilizes reference power supply chip LM4040 to provide fixed voltage VDD/2 for the positive phase input end of operational amplifier OPA4364, and the voltage is applied to the negative phase input end through 'virtual short', and the T-shaped feedback resistance network can realize different amplification factors through resistance impedance adjustment.
As shown in FIG. 3, the multi-channel electrochemical impedance detection module circuit can select different channels to output measurement ranges through a multi-way switch, so that the measurement of objects to be measured with different concentrations is realized, and the measurement result can pass through I of an STM32 chip 2 The C interface data lines SCL and SDA.
The Bluetooth transmission module: the impedance detection result can be displayed on the liquid crystal display module in real time, and can also be connected with the Android smart phone through the wireless Bluetooth module to perform data transmission, the impedance detection result is stored in the mobile phone in a database mode and used for data management and query, and the impedance detection result can be transmitted to a cloud end and sent to a large detection mechanism. The Bluetooth module adopts an HC-05 module, designs and utilizes a CSR BC417143B chip and a Bluetooth 2.0 protocol, can set the baud rate within the range of 1200-115200bps, and performs Bluetooth data communication with an STM32 chip through TXD and RXD ports. The module has the advantages of low price, small volume and low power consumption, and can be used for mobile detection equipment.
In the invention, the impedance measurement adopts a high-performance AD5933 chip and a low-impedance amplifier circuit to form an impedance measurement circuit to carry out impedance measurement on the multi-channel electrochemical impedance sensor. The measurement is carried out according to the principle that resistance impedances generated between a working electrode and a reference electrode of the electrochemical impedance sensor by substances to be measured with different concentrations are different. Setting an internal register through an STM32 chip, setting an impedance measurement starting frequency, a frequency increment and an increment number, outputting a peak value of a sine waveform, providing a sine wave excitation waveform through a DDS core of a 27-phase accumulator at a transmitting end for exciting the impedance to be detected between electrodes of a sensor to be detected, inputting a generated electric signal into a receiving stage of an AD5933 chip, performing FFT calculation through an internal current-voltage amplifier, a programmable gain amplifier, a filter and an ADC (analog-to-digital converter), obtaining a real part and an imaginary part of the impedance to be detected, storing the real part and the imaginary part into the internal register, and performing FFT calculation through I 2 The C interface signals SCL and SDA are communicated with the STM32 chip to obtain impedance values, and the corresponding concentration of the object to be measured is obtained through the measured impedance values.
The invention also provides a using method of the device for detecting the hormone, aiming at the requirement of simultaneously detecting various hormones, the multichannel electrochemical impedance sensor is combined, and the rapid high-sensitivity detection of the object to be detected is realized by utilizing the multichannel electrochemical impedance meter based on the intelligent mobile phone. The hormone is used as a detection object, a multichannel electrochemical impedance sensor structure formed by a multichannel working electrode, a pair of reference electrodes and a counter electrode is designed, and the sensor is modified by using a carbon-based nano composite material and different electroactive substances, so that simultaneous detection of multiple hormones is realized.
The standard curve of the hormone impedance can be obtained by an impedance measurement mode, then the standard curve equation is written into a program of an impedance meter, and finally, a corresponding concentration value is obtained according to the standard curve impedance value corresponding to each hormone in the measurement.
Taking the detection of FSH as an example, the specific steps are as follows:
(1) Preparing a sufficient amount of prepared carbon-based nano composite material and a multi-channel electrochemical impedance sensor modified by an antibody of an object to be detected, and repeatedly cleaning the multi-channel electrochemical impedance sensor by using ultrapure water for later use;
(2) Preparing a series of FSH standard antigen solutions by adopting a proportional dilution method: 0 mIU mL -1 、5 mIU mL -1 、25 mIU mL -1 、50 mIU mL -1 ;
(3) The parameters of the multi-channel electrochemical impedance meter are set, and the frequency range is as follows: 1 to 10 5 Hz, measuring FSH standard antigen solutions with different concentrations to obtain corresponding impedance results, fitting according to a Randles circuit, and respectively obtaining impedance values corresponding to the FSH with different concentrations according to the corresponding relation between the impedance values and the impedance curve;
(4) And respectively taking FSH with different concentrations and corresponding impedance values obtained by detection as horizontal and vertical coordinates, obtaining a fitting curve between the FSH concentration and the impedance by using a linear fitting method, and writing the obtained fitting curve into an STM32 main control code for subsequent detection.
The detection steps of the FSH to be detected are as follows:
firstly, a sample solution to be detected is dripped to the sample adding position of the multi-channel electrochemical impedance sensor by using a pipette, the sample to be detected reaches the surface of a multi-channel working electrode through the siphon effect of cellulose filter paper, the multi-channel electrochemical impedance sensor is kept stand for 25 minutes, after an antigen and an antibody fully react, an electrode joint of the multi-channel electrochemical impedance sensor is inserted into a slot of the multi-channel electrochemical impedance meter, impedance detection is carried out according to the detection method in the steps, a corresponding impedance value is obtained according to the detection result of six channels, and the impedance value is brought into an impedance standard curve equation, so that the concentration of an object to be detected can be obtained.
The results of impedance measurements of different concentrations of FSH are shown in fig. 4.
The invention has the beneficial effects that:
(1) The multi-channel electrochemical impedance sensor comprises a plurality of working electrodes, a reference electrode and a counter electrode, can simultaneously carry out impedance detection on six different objects to be detected, and has high detection efficiency.
(2) The invention adopts a high-precision electrochemical impedance measurement method, and can realize impedance measurement of different modification layers, thereby realizing detection of measured objects with different concentrations.
(3) The impedance meter provided by the invention adopts the Bluetooth wireless module to realize communication between the portable impedance meter and the Android smart phone, and can display the acquired data on the smart phone in real time and perform data processing and management. The use of the Bluetooth wireless module breaks through the restriction of traditional wired communication, can realize wireless communication of data, and greatly expands the application range of equipment.
(4) Low-cost low-power consumption design: the audio interface is used as a power supply mode, so that the defect of a traditional short board for battery endurance is overcome, and the power consumption of the equipment can be greatly reduced by the audio power supply mode; and meanwhile, the USB and the button battery are used as standby power supplies, so that more power supply modes are provided for the equipment. The equipment adopts an ARM STM32F103RCT6 chip with low power consumption, and the inside of the chip comprises a 12-bit ADC and a DAC, so that the design cost is greatly reduced compared with the design mode of singly adopting the ADC and the DAC; and the power consumption of the whole equipment is further reduced by selecting the low-power-consumption Bluetooth module.
Claims (5)
1. An electrochemical impedance meter based on a smart phone is characterized by comprising a multi-channel electrochemical impedance sensor, an electrochemical impedance detection module and an Android smart phone;
the multi-channel electrochemical impedance sensor comprises a plurality of channel working electrodes, a pair of reference electrodes and a pair of counter electrodes; the multi-channel working electrode selects different working electrode channels through a rotary selector switch;
the electrochemical impedance detection module comprises an STM32 main control module, a plurality of single-channel electrochemical impedance modules in communication connection with the STM32 main control module, a Bluetooth transmission module, a liquid crystal display module, a matrix keyboard module and a power supply module;
the electrochemical impedance module is in communication connection with an electrode channel of the electrochemical impedance sensor and comprises an AD5933 chip, a low impedance amplifying circuit and a peripheral circuit;
the STM32 main control module drives and controls the electrochemical impedance module, and transmits detection data to the Android smart phone through a Bluetooth transmission module;
the Android smart phone displays the detection result in a graph and numerical form, and stores data.
2. The smart-phone-based electrochemical impedance meter according to claim 1, wherein the surface of the working electrode of the electrochemical impedance sensor is modified with a carbon-based nanocomposite material.
3. The electrochemical impedance meter based on the smart phone as claimed in claim 1, wherein the low impedance amplifying circuit comprises an operational amplifier OPA4364, a T-type feedback resistance network and a capacitor to form an amplifying circuit for amplifying the detection signal.
4. The electrochemical impedance meter based on the smart phone according to claim 1, wherein the STM32 main control module selects an ARM STM32F103RCT6 chip with low power consumption, and adopts an audio interface as a power supply mode.
5. The method for using the electrochemical impedance meter to detect hormones as claimed in any one of claims 1 to 4, comprising:
(1) Modifying a working electrode of the multi-channel electrochemical impedance sensor by the carbon-based nano composite material and the antibody of the object to be detected, and then repeatedly cleaning by ultrapure water;
(2) Preparing a series of hormone standard antigen solutions by adopting a proportional dilution method;
(3) Parameter setting is carried out on an electrochemical impedance meter, hormone standard antigen solutions with different concentrations are measured, corresponding impedance results are obtained, fitting is carried out according to a Randles circuit, and impedance values corresponding to hormones with different concentrations are respectively obtained according to the corresponding relation between the impedance values and impedance curves;
(4) Respectively taking hormones with different concentrations and corresponding impedance values obtained by detection of the hormones as horizontal and vertical coordinates, obtaining a fitting curve between the concentration of the hormones and the impedance by using a linear fitting method, and writing the obtained fitting curve into an STM32 main control code for subsequent detection;
(5) And (3) dropwise adding a sample solution to be detected to the sample adding position of the multi-channel electrochemical impedance sensor by using a pipette, enabling the sample to be detected to reach the surface of the multi-channel working electrode through the siphon effect of cellulose filter paper, standing for a preset time, inserting an electrode connector of the multi-channel electrochemical impedance sensor into a slot of an electrochemical impedance instrument after the antigen and the antibody fully react, carrying out impedance detection according to the detection methods in the steps (2) and (3), obtaining a corresponding impedance value according to the detection result of the multi-channel, and bringing the impedance value into an impedance standard curve equation so as to obtain the concentration of the object to be detected.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211373912.4A CN115452905A (en) | 2022-11-04 | 2022-11-04 | Electrochemical impedance meter based on smart phone and use method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211373912.4A CN115452905A (en) | 2022-11-04 | 2022-11-04 | Electrochemical impedance meter based on smart phone and use method |
Publications (1)
Publication Number | Publication Date |
---|---|
CN115452905A true CN115452905A (en) | 2022-12-09 |
Family
ID=84310427
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202211373912.4A Pending CN115452905A (en) | 2022-11-04 | 2022-11-04 | Electrochemical impedance meter based on smart phone and use method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN115452905A (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN203933543U (en) * | 2014-05-17 | 2014-11-05 | 徐云鹏 | A kind of T-shaped filter amplification circuit of fast detector |
CN107255665A (en) * | 2017-07-03 | 2017-10-17 | 武汉科技大学 | A kind of mancarried device of quick detection human body female hormone and progestational hormone |
CN111404491A (en) * | 2020-03-25 | 2020-07-10 | 杭州顾宸科技有限公司 | T-shaped resistance network trans-impedance amplifying circuit with automatic voltage compensation function |
CN111912976A (en) * | 2020-06-30 | 2020-11-10 | 无锡市妇幼保健院 | Electrochemical impedance biosensor and preparation method and application thereof |
CN113281386A (en) * | 2021-04-01 | 2021-08-20 | 中山大学 | Multi-channel electrochemical sensor detection device and detection method thereof |
-
2022
- 2022-11-04 CN CN202211373912.4A patent/CN115452905A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN203933543U (en) * | 2014-05-17 | 2014-11-05 | 徐云鹏 | A kind of T-shaped filter amplification circuit of fast detector |
CN107255665A (en) * | 2017-07-03 | 2017-10-17 | 武汉科技大学 | A kind of mancarried device of quick detection human body female hormone and progestational hormone |
CN111404491A (en) * | 2020-03-25 | 2020-07-10 | 杭州顾宸科技有限公司 | T-shaped resistance network trans-impedance amplifying circuit with automatic voltage compensation function |
CN111912976A (en) * | 2020-06-30 | 2020-11-10 | 无锡市妇幼保健院 | Electrochemical impedance biosensor and preparation method and application thereof |
CN113281386A (en) * | 2021-04-01 | 2021-08-20 | 中山大学 | Multi-channel electrochemical sensor detection device and detection method thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101579236A (en) | Multi-frequency and multi-segment measuring device and measuring method of human body impedance | |
CN209342650U (en) | Water environment trace element detection device based on electrochemistry | |
CN106370714A (en) | Mobile terminal-based cyclic voltammetry biochemical detection device and method | |
CN103149441A (en) | Portable impedance spectrum analyzer applied to electrochemical measurement and impedance spectrum analyzing method | |
CN109374694A (en) | A kind of Multi-channel hand-held electrochemical instrument | |
CN202891910U (en) | Portable blood sugar blood pressure determinator | |
CN104931558A (en) | Universal POCT (point-of-care testing) apparatus based on intelligent cellphone platform and test method thereof | |
CN115452905A (en) | Electrochemical impedance meter based on smart phone and use method | |
CN110007056B (en) | Novel low-power-consumption wireless portable water quality detection system | |
CN201107295Y (en) | Portable twin channel electrochemistry analytical equipment | |
CN117288820A (en) | Three-way electrochemical sensing system and method based on Internet of things communication | |
CN202693518U (en) | Dissolved oxygen monitoring system based on polarographic electrode | |
CN104614405B (en) | Mobile bioelectrical impedance sensing device and method for TNT detection | |
CN203117280U (en) | STM 32-based intelligent four-probe meter | |
CN207751928U (en) | A kind of residual electrochemical analyser of agriculture | |
CN101089621A (en) | Portable two-channel electrochemical analysing equipment | |
CN214310656U (en) | Centralized measuring system | |
CN202255529U (en) | Liquid volume measuring device | |
CN211124351U (en) | Multifunctional data acquisition unit based on simulation and digital acquisition | |
CN205881273U (en) | Wireless sensor network tests platform | |
CN109212001B (en) | Detection device and method | |
CN206725628U (en) | Multifunctional portable electronic tests virtual instrument | |
CN109374971B (en) | Megahertz impedance meter based on embedded system | |
CN110672793A (en) | Multi-parameter microenvironment air quality monitoring device capable of achieving rapid and accurate detection | |
CN214252429U (en) | STM 32-based modular portable impedance testing system |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20221209 |