CN115452787A - Method for measuring streptomycin in milk by using fluorescence sensor constructed by silver nanoclusters and gold palladium nanoparticles - Google Patents

Method for measuring streptomycin in milk by using fluorescence sensor constructed by silver nanoclusters and gold palladium nanoparticles Download PDF

Info

Publication number
CN115452787A
CN115452787A CN202211154362.7A CN202211154362A CN115452787A CN 115452787 A CN115452787 A CN 115452787A CN 202211154362 A CN202211154362 A CN 202211154362A CN 115452787 A CN115452787 A CN 115452787A
Authority
CN
China
Prior art keywords
dna
pdnps
agncs
milk
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211154362.7A
Other languages
Chinese (zh)
Inventor
李发兰
王晓阳
田宇航
孙霞
郭业民
杨青青
张妍妍
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong University of Technology
Original Assignee
Shandong University of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong University of Technology filed Critical Shandong University of Technology
Priority to CN202211154362.7A priority Critical patent/CN115452787A/en
Publication of CN115452787A publication Critical patent/CN115452787A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B22CASTING; POWDER METALLURGY
    • B22FWORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
    • B22F9/00Making metallic powder or suspensions thereof
    • B22F9/16Making metallic powder or suspensions thereof using chemical processes
    • B22F9/18Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
    • B22F9/24Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00Nanotechnology for materials or surface science, e.g. nanocomposites
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00Manufacture or treatment of nanostructures
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432Quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Manufacturing & Machinery (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The invention provides a preparation method of a fluorescence aptamer sensor for detecting Streptomycin (STR) residues in milk, belonging to the field of food safety detection. The invention comprises a method for synthesizing a hairpin type DNA-silver nano cluster (DNA-AgNCs) regulated and controlled by guanine (G) base, synthesizing core-shell gold-palladium nano particles (Au @ PdNPs) and constructing a fluorescent sensor. According to the invention, different numbers of G bases are added into the hairpin loop, so that the red shift of the emission spectrum and the increase of the fluorescence intensity of DNA-AgNCs are realized. The Au @ PdNPs is prepared by a seed growth method and a deposition method, and the Au @ PdNPs composite material not only solves the problem that the quenching capacity of the palladium nanoparticles (PdNPs) is weak in the long wavelength range, but also effectively expands the fluorescence quenching range of the gold nanoparticles (AuNPs). The inventive sensor responded well to STRs in the range of 50-1250 nM with a limit of detection (LOD) of 18.7 nM. In addition, the invention has been successfully applied to STR detection in milk, and shows that the invention has good application prospect in STR detection in the field of animal-derived foods.

Description

Method for measuring streptomycin in milk by using fluorescence sensor constructed by silver nanoclusters and gold palladium nanoparticles
Technical Field
The invention provides a preparation method of an aptamer sensor for detecting streptomycin residue in milk, and belongs to the technical field of food safety detection.
Background
Streptomycin (STR) is a common aminoglycoside antibiotic, is used for treating mastitis and other diseases caused by bacterial infection of cows, and can cause STR residues in dairy products such as milk if unreasonably used, thereby not only affecting the quality of the dairy products, but also causing serious harm to human bodies, including allergy, damage to intestinal flora and toxicity to kidneys.
Therefore, it is crucial to develop sensitive and specific technologies for detecting STR in foods of animal origin, such as milk, and at present, sensitive detection methods such as capillary electrophoresis, high performance liquid chromatography and enzyme-linked immunosorbent assay have been developed for detecting STR in milk residues, but their application is limited due to the disadvantages of long turnaround time, complicated steps, expensive equipment and high technical requirements.
Disclosure of Invention
The invention aims to establish a rapid, simple and efficient detection technology for detecting STR in milk.
The technical scheme is as follows: aptamers are high-affinity and specific RNA or ssDNA sequences screened by a number-enriched ligand systematic evolution technology (SELEX), and in recent years, due to the advantages of rapidness, sensitivity and easiness in operation, fluorescent sensors based on aptamers are widely applied.
The preparation method of the fluorescent aptamer sensor for detecting STR residues in milk (figure 1) is characterized by comprising the following steps: the method is characterized in that a G base group is added into a hairpin type DNA-silver nanocluster (DNA-AgNCs) ring to adjust the fluorescence spectrum of the DNA-AgNCs, a template chain of the DNA-AgNCs contains an STR aptamer (STP) sequence, the aptamer of the DNA-AgNCs is adsorbed on the surface of gold palladium nanoparticles (Au @ PdNPs) through coordination under the condition of no STR, therefore, energy is transferred from the fluorescence donor DNA-AgNCs to the Au @ PdNPs to cause fluorescence quenching, when the STR is added, the conformation of the aptamer is changed due to specific combination of the STR and the aptamer, the interaction between the aptamer and the Au @ PdNPs is weakened, the fluorescence signal is recovered, and the STR in the milk can be successfully detected based on the change of the fluorescence signal.
The preparation method of the fluorescent aptamer sensor for detecting STR residues in milk is characterized by comprising the following steps: in addition, the Au @ PdNPs composite material with excellent quenching property is synthesized, so that the problem of weak quenching capability of palladium nanoparticles (PdNPs) in a long wavelength range is solved, and the fluorescence quenching range of gold nanoparticles (AuNPs) is effectively expanded.
The preparation principle is as follows: on the basis of Fluorescence Resonance Energy Transfer (FRET), a fluorescence sensor utilizing DNA-AgNCs and core-shell gold-palladium nanoparticles (Au @ PdNPs) is designed to detect Streptomycin (STR) in milk, and an aptamer of the DNA-AgNCs is adsorbed on the surface of the Au @ PdNPs through coordination. DNA-AgNCs and Au @ PdNPs generate FRET, energy is transferred from fluorescence donor DNA-AgNCs to Au @ PdNPs, fluorescence quenching is caused, when STR is added, the specific binding of the STR and the aptamer causes the conformation of the aptamer to change, the FRET between the aptamer and the Au @ PdNPs is destroyed, and the recovery of fluorescence signals is caused.
In order to achieve the purpose, the following technical scheme is adopted for realizing the purpose: silver nanoclusters were prepared by first preparing all DNA template solutions (100. Mu.M) with TE buffer and holding in a water bath (95 ℃) for 10 minutes, then cooling the solution to room temperature in an ice-water bath, followed by 500. Mu.L of 100. Mu.M DNA solution and 300. Mu.L of 1 mM silver nitrate (AgNO) 3 ) The solutions were added to 3.9 ml of TE buffer, mixed thoroughly and shaken, all the mixed solutions were incubated in ice bath and in the dark for 30 minutes, and 300. Mu.L of 1 mM freshly prepared and ice-cold sodium borohydride (NaBH) was added to the mixed solutions, respectively 4 ) Solution, after the reaction is finished, fully shaking the mixed solution for 1 minute, and finally obtaining the productStoring the obtained solution at 4 deg.C in dark place, preparing Au @ PdNPs, and mixing 100 ml of 0.01% (w/v) gold chloride trihydrate (HAuCl) 4 ·3H 2 O) solution was heated to boiling, after which 2.7 mL of 1% (w/v) trisodium citrate (Na) were added rapidly 3 C 6 H 5 O 7 ) The solution was continuously heated and boiled until the color of the gold seed solution did not change, and then the gold seed solution was cooled to room temperature under constant stirring, followed by mixing 2.0 mL of the gold seed solution and 4 mL of 1% (w/v) HAuCl under vigorous stirring 4 ·3H 2 The O solution was added to 200 mL of ultrapure water, and after stirring well, 800. Mu.L of 1% (w/v) Na was added 3 C 6 H 5 O 7 Solution and 400. Mu.L of 30 mM Hydroquinone (C) 6 H 6 O 2 ) The solution is quickly added into the mixed solution for reduction. The above reducing agent was repeatedly added to the mixed solution 7 times at intervals of 10 minutes, and after the addition was completed, the mixed solution was further stirred for 1 hour, and finally, 1 mM H was synthesized by the following method 2 PdCl 4 Solution, 10.64 mg of palladium chloride (PdCl) 2 ) Is dissolved in 6 mL of 0.02M HCl solution and 54 mL of ultrapure water are added to the mixture in a water bath at 70 ℃ until PdCl 2 Complete dissolution to give 1 mM H 2 PdCl 4 Solutions of Au @ PdNPs obtained by deposition of Palladium, 30 mL of 75 nm AuNPs solution with 1 mL, 4 mL, 7 mL and 10 mL of H, respectively 2 PdCl 4 The solutions (1 mM) were mixed, the mixed solution was cooled in an ice bath, 1 mL, 4 mL, 7 mL and 10 mL ascorbic acid solution and 18 mL, 12 mL, 6 mL and 0 mL ultrapure water were respectively and slowly added to the mixed solution under vigorous stirring, then the mixed solution was further stirred for 30 minutes, after stirring, the resulting solution was centrifuged, washed with water-ethanol solution (1, 2, v/v) several times, and finally, au @ PdNPs were dissolved in C 6 H 5 Na 3 O 7 In solution (0.02%, w/v).
In order to achieve the purpose, the following technical scheme is adopted to realize the purpose: before detecting STR, the concentration of hairpin DNA-AgNCs is diluted to 1 μ M and the excitation wavelength is 586 nm in the whole experiment process, and the detection result of STR is obtained under the optimal experiment condition, 500 μ L of DNA-AgNCs and 500 μ L of Au @ PdNPs solution are mixed for 15 minutes, a series of STR solutions with different concentrations of 500 μ L are respectively added into the mixed solution of DNA-AgNCs and Au @ PdNPs, the reaction lasts for 60 minutes, and then the fluorescence intensity is measured.
The preparation process of the aptamer sensor comprises the following steps: STRs were added to milk at three concentration levels (300 nM, 600 nM and 900 nM) before pretreatment, and subsequently, the milk sample was pretreated by the following method, 20 mL of a milk-methanol (1, 4, v/v) mixture was kept at-20 ℃ for 20 minutes, then the mixture was centrifuged at 12500 rpm for 25 minutes, the supernatant was filtered through a membrane filter (0.22 μm), after filtration, the supernatant was concentrated and dried in a water bath at 60 ℃, the residue was dried with nitrogen and then reconstituted with ultrapure water, the reconstituted solution was filtered through a membrane filter (0.22 μm), and finally, the volume of the filtrate was set to 4 mL.
Drawings
FIG. 1 shows a process for constructing a fluorescence aptamer sensor.
FIG. 2 excitation and emission wavelengths of DNA-AgNCs synthesized from different template strands.
FIG. 3 fluorescence spectrum and fluorescence intensity of DNA-AgNCs.
FIG. 4 Electron microscopy characterization of nanomaterials.
Fig. 5 optimization of palladium shell thickness.
Figure 6 experimental conditions were optimized.
FIG. 7 fluorescent determination of STR.
FIG. 8 specific assay.
FIG. 9 STR testing in real samples of milk.
Detailed Description
Example 1: FIG. 3A shows fluorescence spectra of DNA-AgNCs of different template strand synthesis, excitation and emission wavelengths are listed in FIG. 2, and fluorescence intensity is shown in FIG. 3B, however, in DNA 2 、DNA 3 And DNA 4 、DNA 5 In (2), there is no regular red shift as the number of G bases increases, and when the number of G bases reaches 10 and 12, the red shift no longer occurs, when the number of bases is 0 to 6In time, the fluorescence intensity did not change much, however, when the number of G bases increased to 8, significant enhancement and change occurred due to DNA 4 -AgNCs、DNA 5 -AgNCs、DNA 6 AgNCs and DNA 7 The emission spectrum of AgNCs is close to the near infrared region, which means that it requires large metal nanoparticles, but larger metal nanoparticles may cause scattering, leading to a distortion of the fluorescence spectrum, DNA 3 Fluorescence intensity of AgNCs in DNA 1 -AgNCs、DNA 2 AgNCs and DNA 3 The strongest of the AgNCs, DNA 3 -position ratio DNA of maximum emission peak of AgNCs 2 The AgNCs are short, so the quencher nanoparticles required are smaller in diameter and less susceptible to scattering, and in summary, DNA was selected 3 AgNCs as fluorescent material used in this experiment.
Example 2: DNA Observation by TEM 3 Morphology of AgNCs, DNA synthesized as shown in FIG. 4A 3 AgNCs showed a uniform dispersion, almost circular, with an average size of 2.47 nm, and measured for DNA 3 Stability of AgNCs over 120 hours, results show, DNA 3 AgNCs are stable for 12-24 hours, so the experiment is on DNA 3 Measured DNA, performed within 12-24 hours after AgNCs synthesis 3 Absolute quantum yield of-AgNCs is 10.76%, TEM image of gold seeds is shown in FIG. 4B, from which it can be seen that the gold seeds are spherical particles with diameter of 19.9 nm, FIG. 4E shows large size gold nanoparticles synthesized by seed growth method with diameter of 75 nm, from FIGS. 4F-I, with different amount of H 2 PdCl 4 And ascorbic acid on the surface of AuNPs, it can be seen that the thickness of the palladium shell is about 4 nm, 7 nm, 11 nm and 16 nm, respectively, FIG. 4J-M shows EDX of Au @ PdNPs with diameter of 75+16 nm, it can be seen that palladium is dispersed in the outer layer of the gold core, the weight ratio of gold/palladium is 33.36/66.64, FIG. 4C, D shows SEM of Au @ PdNPs with diameter of 75+16 nm, according to the image, au @ PdNPs are almost spherical particles with diameter of 107.11 nm, the above analysis shows that Au @ PdNPs and DNA are almost spherical particles 3 The synthesis effect of the AgNCs is good.
Example 3: the optimization of the thickness of the palladium shell (FIG. 5), the energy transfer efficiencies (E) of 75+4 nm, 75+7 nm, 75+11 nm and 75+16 nm Au @ PdNPs are respectively 26.7%, 46.6%, 56.7% and 62.8%, it can be found that the energy transfer efficiency is gradually increased along with the increase of the thickness of the palladium shell, the 75+16 nm Au @ PdNPs has very high energy transfer efficiency which is enough to meet the experimental requirements, and meanwhile, the interference of metal nanoparticle scattering is increased by further increasing the particle size, so the 75+16 nm Au @ PdNPs with the highest quenching efficiency is selected as the quenching agent of the experiment.
Example 4: optimization of the conditions in the assay (FIG. 6), (A) optimization of the pH in the DNA-AgNCs synthesis (6.5-9.0), pH optimum 7.5, (B) addition of 0-24.8 pm concentrations of Au @ PdNPs to 1. Mu.M DNA 3 In AgNCs, the optimal concentration of Au @ PdNPs is 17.2 pM, (C) the incubation time for fluorescence quenching (0-30 min), the optimal quenching time is 15min, and (D) the incubation time for fluorescence recovery (0-90 min), and the optimal recovery time is 60min.
Example 5: the performance of the sensor for determining the analyte is evaluated by measuring STRs with different concentrations, as shown in FIG. 7, the fluorescence intensity gradually increases with the increase of the STR concentration within the range of 50 to 2800 nM, the fluorescence intensity and the STR concentration have a positive linear relation within the range of 50 to 1250 nM, and the equation of a linear calibration curve is as follows: i =0.43314C STR +102.94683, correlation coefficient (R) 2 ) 0.99258, with a limit of detection (LOD) of the STR fluorometry of 18.7 nM according to 3 σ/k, where σ is the standard deviation of the measurement of the calibration standard blank (n = 10) and k is the slope of the calibration curve.
Example 6: to evaluate the specificity of the sensor, the specificity of the DNA-AgNCs-based fluorescence aptamer was determined by comparison with other antibiotics such as Telavancin (TER), chloramphenicol (CHL), tetracycline (TET), erythromycin (ERY), chlortetracycline hydrochloride (CHH) and Penicillin (PEN), at a concentration 10 times that of STR, as shown in FIG. 8, I and I 0 Indicating the fluorescence intensity in the presence and absence of different types of antibiotics, respectively, and that STR responds to fluorescence aptamers more strongly than other antibiotics, these results indicate that fluorescence adaptationThe instrument has strong specificity for detecting STR, and shows that the developed fluorescence adapter can be used for detecting STR.
Example 7: to evaluate the feasibility of this invention to detect STR in milk samples, we added three different concentrations of streptomycin to actual milk, and the recovery is shown in fig. 9, and it can be seen that the peak recovery of kanamycin detection is between 97.45% and 105.34%, and the Relative Standard Deviation (RSD) is between 1.05% and 3.04%, and these excellent properties indicate the feasibility of our proposed streptomycin detection method in this application.

Claims (4)

1. A preparation method of a fluorescence aptamer sensor for detecting streptomycin in milk is characterized by comprising the following steps: a fluorescence aptamer sensor based on guanine (G) base-regulated hairpin-type DNA silver nanoclusters (DNA-AgNCs) and core-shell gold-palladium nanoparticles (Au @ PdNPs) is constructed and used for detecting Streptomycin (STR).
2. The method for preparing an aptamer sensor for detecting streptomycin in milk according to claim 1, wherein the aptamer sensor comprises: different numbers of G bases were added to the hairpin loop to achieve a red shift in the emission spectrum and an increase in the fluorescence intensity of the DNA-AgNCs.
3. The method of claim 1, wherein the aptamer sensor for detecting streptomycin in milk comprises: the excellent quenching material Au @ PdNPs is prepared by a seed growth method and a deposition method, the Au @ PdNPs and the DNA-AgNCs are utilized to carry out fluorescence resonance energy transfer, and the streptomycin is detected by the change of fluorescence signals of the Au @ PdNPs and the DNA-AgNCs under the corresponding excitation wavelength.
4. The method of claim 1, wherein the aptamer sensor for detecting streptomycin in milk comprises: the sensor has better sensing performance, and can detect STR residues in an actual milk sample.
CN202211154362.7A 2022-09-22 2022-09-22 Method for measuring streptomycin in milk by using fluorescence sensor constructed by silver nanoclusters and gold palladium nanoparticles Pending CN115452787A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211154362.7A CN115452787A (en) 2022-09-22 2022-09-22 Method for measuring streptomycin in milk by using fluorescence sensor constructed by silver nanoclusters and gold palladium nanoparticles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211154362.7A CN115452787A (en) 2022-09-22 2022-09-22 Method for measuring streptomycin in milk by using fluorescence sensor constructed by silver nanoclusters and gold palladium nanoparticles

Publications (1)

Publication Number Publication Date
CN115452787A true CN115452787A (en) 2022-12-09

Family

ID=84306013

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211154362.7A Pending CN115452787A (en) 2022-09-22 2022-09-22 Method for measuring streptomycin in milk by using fluorescence sensor constructed by silver nanoclusters and gold palladium nanoparticles

Country Status (1)

Country Link
CN (1) CN115452787A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115894624A (en) * 2022-12-29 2023-04-04 吉林大学 Gold nanocluster using polypeptide as ligand and detection method of chlortetracycline hydrochloride
CN116200538A (en) * 2022-12-12 2023-06-02 盐城工学院 Application of nano kite sensor in preparation of multi-target virus gene detection reagent

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140024137A1 (en) * 2012-07-11 2014-01-23 Nubad, LLC Methods and compositions related to nucleic acid binding assays
US20150240297A1 (en) * 2012-07-31 2015-08-27 Juan Carlos Jaime Method for obtaining and detecting a marker of objects to be identified, related marker, authentication method and verification method
US20170166890A1 (en) * 2013-06-14 2017-06-15 The University Of Notre Dame Dnazyme-nanoparticle conjugates and methods of use thereof
CN108315421A (en) * 2018-04-04 2018-07-24 山东师范大学 Method of the constant-temperature amplification with the combination of quantum dot fluorescence Resonance energy transfer for detecting a variety of MicroRNAs simultaneously
CN108445031A (en) * 2018-04-26 2018-08-24 山东理工大学 A kind of preparation method of aptamer sensor that is while detecting kanamycins and Determination of Streptomycin Residues
WO2021004379A1 (en) * 2019-07-05 2021-01-14 南京金斯瑞生物科技有限公司 Fluorescently labeled nucleic acid and synthesis method therefor
CN112893864A (en) * 2021-01-20 2021-06-04 江南大学 Silver nanocluster prepared based on hairpin template and application of silver nanocluster in chloramphenicol detection
WO2021145796A2 (en) * 2020-01-14 2021-07-22 Максим Петрович НИКИТИН Method of determining analyte content in a sample with the aid of a nanoparticle-based agent and a recognition oligonucleotide
WO2021175762A1 (en) * 2020-03-03 2021-09-10 Universiteit Gent Hydrolysis-based probe and method for str genotyping
CN114136944A (en) * 2021-12-10 2022-03-04 山东理工大学 Preparation method of fluorescence aptamer sensor for detecting kanamycin residue in milk
CN114518359A (en) * 2022-03-08 2022-05-20 山东理工大学 Preparation method of G-quadruplet-based dual-mode kanamycin aptamer sensor
CN114624223A (en) * 2022-03-15 2022-06-14 山东农业大学 Method for detecting streptomycin based on surface enhanced Raman spectroscopy sensor embedded with specific nuclease DNA hydrogel

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140024137A1 (en) * 2012-07-11 2014-01-23 Nubad, LLC Methods and compositions related to nucleic acid binding assays
US20150240297A1 (en) * 2012-07-31 2015-08-27 Juan Carlos Jaime Method for obtaining and detecting a marker of objects to be identified, related marker, authentication method and verification method
US20170166890A1 (en) * 2013-06-14 2017-06-15 The University Of Notre Dame Dnazyme-nanoparticle conjugates and methods of use thereof
CN108315421A (en) * 2018-04-04 2018-07-24 山东师范大学 Method of the constant-temperature amplification with the combination of quantum dot fluorescence Resonance energy transfer for detecting a variety of MicroRNAs simultaneously
CN108445031A (en) * 2018-04-26 2018-08-24 山东理工大学 A kind of preparation method of aptamer sensor that is while detecting kanamycins and Determination of Streptomycin Residues
WO2021004379A1 (en) * 2019-07-05 2021-01-14 南京金斯瑞生物科技有限公司 Fluorescently labeled nucleic acid and synthesis method therefor
WO2021145796A2 (en) * 2020-01-14 2021-07-22 Максим Петрович НИКИТИН Method of determining analyte content in a sample with the aid of a nanoparticle-based agent and a recognition oligonucleotide
WO2021175762A1 (en) * 2020-03-03 2021-09-10 Universiteit Gent Hydrolysis-based probe and method for str genotyping
CN112893864A (en) * 2021-01-20 2021-06-04 江南大学 Silver nanocluster prepared based on hairpin template and application of silver nanocluster in chloramphenicol detection
CN114136944A (en) * 2021-12-10 2022-03-04 山东理工大学 Preparation method of fluorescence aptamer sensor for detecting kanamycin residue in milk
CN114518359A (en) * 2022-03-08 2022-05-20 山东理工大学 Preparation method of G-quadruplet-based dual-mode kanamycin aptamer sensor
CN114624223A (en) * 2022-03-15 2022-06-14 山东农业大学 Method for detecting streptomycin based on surface enhanced Raman spectroscopy sensor embedded with specific nuclease DNA hydrogel

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WANG, XIAOYANG ETAL.: "A novel fluorescence sensor for streptomycin detection based on hairpin DNA-AgNCs and core-shell gold-palladium nanoparticles", 《SENSORS AND ACTUATORS B-CHEMICAL》, vol. 373, 15 December 2022 (2022-12-15) *
阿说阿沙 等: "荧光免疫分析法在牛奶抗生素残留检测中的研究进展", 《中国兽医杂志》, vol. 55, no. 09, 30 September 2019 (2019-09-30) *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116200538A (en) * 2022-12-12 2023-06-02 盐城工学院 Application of nano kite sensor in preparation of multi-target virus gene detection reagent
CN115894624A (en) * 2022-12-29 2023-04-04 吉林大学 Gold nanocluster using polypeptide as ligand and detection method of chlortetracycline hydrochloride
CN115894624B (en) * 2022-12-29 2023-12-08 吉林大学 Gold nanocluster with polypeptide as ligand and detection method of aureomycin hydrochloride

Similar Documents

Publication Publication Date Title
CN115452787A (en) Method for measuring streptomycin in milk by using fluorescence sensor constructed by silver nanoclusters and gold palladium nanoparticles
Liu et al. Construction of biomass carbon dots based fluorescence sensors and their applications in chemical and biological analysis
Li et al. Designing an aptamer based magnetic and upconversion nanoparticles conjugated fluorescence sensor for screening Escherichia coli in food
CN113155807B (en) MicroRNA ultrasensitive detection method based on surface enhanced Raman spectroscopy technology
Zhou et al. Gold nanobones enhanced ultrasensitive surface-enhanced Raman scattering aptasensor for detecting Escherichia coli O157: H7
Lu et al. Target-driven switch-on fluorescence aptasensor for trace aflatoxin B1 determination based on highly fluorescent ternary CdZnTe quantum dots
Kong et al. Carbon dots for fluorescent detection of α-glucosidase activity using enzyme activated inner filter effect and its application to anti-diabetic drug discovery
Wang et al. Magnetic plasmonic particles for SERS-based bacteria sensing: A review
Jayan et al. Mesoporous silica coated core-shell nanoparticles substrate for size-selective SERS detection of chloramphenicol
Wei et al. A novel gold nanostars-based fluorescent aptasensor for aflatoxin B1 detection
Ren et al. A novel fluorescence resonance energy transfer (FRET)-based paper sensor with smartphone for quantitative detection of Vibrio parahaemolyticus
CN108535236B (en) Method for ultrasensitively detecting miRNA based on dual-amplification SERS signal system
US8263668B2 (en) Tunable fluorescent gold nanocluster and method for forming the same
CN110455756B (en) Method for simultaneously detecting divalent lead ions and divalent copper ions
CN113005180A (en) Magnetic SERS biosensor and preparation method and application thereof
CN115825037B (en) Preparation method and application of hydrogel-loaded gold nanoparticle SERS substrate
CN109387500B (en) Method for detecting escherichia coli based on magnetic graphene oxide composite star @ gold-silver alloy nanoparticles
CN109303923B (en) Method for preparing nano-cluster gel containing hydroxyapatite-like component
KR20210019767A (en) Magnetic-Optical Composite Nanoparticles
Li et al. Recent advances in rare earth ion‐doped upconversion nanomaterials: From design to their applications in food safety analysis
WO2010114297A2 (en) Microsphere having hot spots and method for identifying chemicals through surface enhanced raman scattering using the same
Wang et al. A novel fluorescence sensor for streptomycin detection based on hairpin DNA-AgNCs and core-shell gold-palladium nanoparticles
Gao et al. A sensitive colorimetric aptasensor for chloramphenicol detection in fish and pork based on the amplification of a nano-peroxidase-polymer
Kumar et al. Fast tyrosinase detection in early stage melanoma with nanomolar sensitivity using a naphthalimide-based fluorescent read-out probe
CN112710649B (en) Method for detecting kanamycin sulfate by using dual-signal-enhanced surface-enhanced Raman spectroscopy

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination