CN115433712A - Umbilical cord mesenchymal stem cell proliferation culture medium and culture method - Google Patents
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Abstract
The invention discloses an umbilical cord mesenchymal stem cell proliferation culture medium, which comprises a basic culture medium and an additive, wherein the additive comprises the following components: eicosapentaenoic acid, piperine, retinervus Luffae fructus powder, glutathione, sodium selenite, epidermal growth factor, and vitamin C. The proliferation culture medium provided by the invention is added with the synergistic effect of the components such as eicosapentaenoic acid, piperine, loofah sponge powder and the like, animal serum is not required to be added, the proliferation speed of the umbilical cord mesenchymal stem cells can be effectively improved, the adherence time is shortened, the cell characteristics are not changed, a large amount of umbilical cord mesenchymal stem cells can be obtained after short-time amplification, and the application of the umbilical cord mesenchymal stem cells in various fields is fully ensured. The invention also provides a culture method of the umbilical cord mesenchymal stem cells, and the culture process is simple and convenient and easy to operate by adopting the proliferation culture medium provided by the invention, so that the cost of the umbilical cord mesenchymal stem cells in vitro culture is effectively reduced.
Description
Technical Field
The invention relates to stem cells, in particular to a proliferation culture medium and a culture method for umbilical cord mesenchymal stem cells.
Background
Mesenchymal Stem Cells (MSCs) are currently the most promising seed cells, derived from early-developing mesoderm and ectoderm, and have the ability of self-renewal, proliferation and multidirectional differentiation, and MSCs, as a class of undifferentiated cells with the potential of self-renewal and multidirectional differentiation, have been proved to have strong ability to repair and regenerate damaged tissues due to the self-renewal, replication and multidirectional differentiation potential.
The mesenchymal stem cells of the bone marrow are the earliest discovered mesenchymal stem cells and are most widely researched, but the quantity and the differentiation and proliferation capacity of the mesenchymal stem cells of the adult are greatly influenced by the age and the health state of an individual, the bone marrow is required to be punctured for sampling, the injury to the body is large, and the operation process is easy to be polluted. Compared with the mesenchymal stem cells of the bone marrow, the mesenchymal stem cells derived from the umbilical cord of the newborn belong to medical waste, are convenient to obtain materials, rich in sources, low in cost, large in quantity and strong in proliferation and differentiation capacity, can be better applied to the aspects of medical treatment and cosmetology, and are more ideal mesenchymal cell sources.
The umbilical cord mesenchymal stem cells have the characteristics of strong proliferation capacity, rapid amplification, difficult aging, multidirectional differentiation potential and the like. In order to ensure the application of umbilical cord mesenchymal stem cells, it is a common technical means to obtain a sufficient number of cells through in vitro proliferation culture. The existing umbilical cord mesenchymal stem cell culture medium is often added with blood products such as animal serum and the like, and the uncertainty and the risk of the culture process are increased, so that the improvement of the proliferation culture medium of the umbilical cord mesenchymal stem cells is necessary, the safety of the culture medium and the efficiency of the in-vitro proliferation of the umbilical cord mesenchymal stem cells are improved, a large amount of umbilical cord mesenchymal stem cells can be obtained in a short time, and the application cost of the umbilical cord mesenchymal stem cells is reduced.
Disclosure of Invention
In order to overcome the defects of the prior art, one of the purposes of the invention is to provide the umbilical cord mesenchymal stem cell proliferation culture medium which is safe in components, improves the proliferation speed and the cell viability of the umbilical cord mesenchymal stem cells, shortens the adherence time, enables the proliferation efficiency of the umbilical cord mesenchymal stem cells to be higher and reduces the culture cost.
The second purpose of the invention is to provide a method for culturing umbilical cord mesenchymal stem cells.
One of the purposes of the invention is realized by adopting the following technical scheme:
an umbilical cord mesenchymal stem cell proliferation culture medium comprises a basic culture medium and an additive, wherein the additive comprises the following components: eicosapentaenoic acid, piperine, loofah sponge powder, glutathione, sodium selenite, epidermal growth factor and vitamin C.
Further, the basic culture medium is a DMEM low-sugar culture medium, and the mass concentration of each component in the additive in the culture medium is as follows: 3.5-6.5ng/mL of eicosapentaenoic acid, 4.2-7.4ng/mL of piperine, 6.5-9.5ng/mL of loofah sponge powder, 10-15 mu g/mL of glutathione, 8.6-10.5ng/mL of sodium selenite, 5-8ng/mL of epidermal growth factor and 1-4 mu g/mL of vitamin C.
Further, the mass concentration of each component in the additive in the culture medium is as follows: 5ng/mL of eicosapentaenoic acid, 6.4ng/mL of piperine, 8.5ng/mL of loofah sponge powder, 12 mu g/mL of glutathione, 9.2ng/mL of sodium selenite, 6ng/mL of epidermal growth factor and 3 mu g/mL of vitamin C.
Further, the fineness of the loofah sponge powder is 100-150 meshes. The preparation method of the loofah sponge powder comprises the following steps: cleaning retinervus Luffae fructus, carbonizing at 175 deg.C in vacuum drying oven for 5 hr, cooling to room temperature, pulverizing, and sieving to obtain retinervus Luffae fructus powder with fineness of 100-150 mesh.
The second purpose of the invention is realized by adopting the following technical scheme:
a culture method of umbilical cord mesenchymal stem cells adopts the proliferation culture medium to culture.
Further, the seeding density of the umbilical cord mesenchymal stem cells in the proliferation culture medium is 1-5 x 10 4 One per mL.
Further, 5% CO at 37 ℃% 2 The cell culture is performed under conditions.
Compared with the prior art, the invention has the beneficial effects that: the invention provides an umbilical cord mesenchymal stem cell proliferation culture medium, which is added with eicosapentaenoic acid, piperine, loofah sponge powder and other components for synergistic effect, does not need to be added with animal serum, can effectively improve the proliferation speed of the umbilical cord mesenchymal stem cells, shortens the adherence time, does not change the cell characteristics, can harvest a large amount of umbilical cord mesenchymal stem cells after being amplified for a short time, and fully ensures the application of the umbilical cord mesenchymal stem cells in various fields.
The invention also provides a culture method of the umbilical cord mesenchymal stem cells, and the culture process is simple and convenient and easy to operate by adopting the proliferation culture medium provided by the invention, so that the cost of the umbilical cord mesenchymal stem cells in vitro culture is effectively reduced.
Drawings
FIG. 1 is a proliferation curve of umbilical cord mesenchymal stem cells in example 1 of the present invention, comparative examples 1 to 5.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that any combination of the embodiments or technical features described below can be used to form a new embodiment without conflict.
Example 1
The proliferation culture medium for umbilical cord mesenchymal stem cells consists of a DMEM low-sugar culture medium and an additive, wherein the additive consists of: eicosapentaenoic acid, piperine, retinervus Luffae fructus powder, glutathione, sodium selenite, epidermal growth factor, and vitamin C; the mass concentration of each component in the additive in the culture medium is as follows: 5ng/mL of eicosapentaenoic acid, 6.4ng/mL of piperine, 8.5ng/mL of loofah sponge powder with the fineness of 100-150 meshes, 12 mu g/mL of glutathione, 9.2ng/mL of sodium selenite, 6ng/mL of epidermal growth factor and 3 mu g/mL of vitamin C.
A culture method of umbilical cord mesenchymal stem cells comprises the following processes: rapidly recovering P3 generation umbilical cord mesenchymal stem cells which are frozen and stored for 1 month and are in logarithmic growth phase in a water bath kettle at 37 ℃, centrifuging to remove frozen stock solution, washing cells with PBS, adding the proliferation culture medium for resuspension, and adjusting the cell density to be 3 multiplied by 10 4 seed/mL, in 96-well plates at 37 ℃,5% CO 2 The culture was carried out in the incubator of (1), with changing the medium every 3 days, and continuously for 7 days.
Example 2
The proliferation culture medium for umbilical cord mesenchymal stem cells consists of a DMEM low-sugar culture medium and an additive, wherein the additive consists of: eicosapentaenoic acid, piperine, retinervus Luffae fructus powder, glutathione, sodium selenite, epidermal growth factor, and vitamin C; the mass concentration of each component in the additive in the culture medium is as follows: 3.5ng/mL of eicosapentaenoic acid, 4.2ng/mL of piperine, 6.5ng/mL of loofah sponge powder with the fineness of 100-150 meshes, 10 mu g/mL of glutathione, 8.6ng/mL of sodium selenite, 5ng/mL of epidermal growth factor and 1 mu g/mL of vitamin C.
A culture method of umbilical cord mesenchymal stem cells comprises the following processes: rapidly recovering P3 generation umbilical cord mesenchymal stem cells in logarithmic growth phase in water bath at 37 deg.C after freezing for 1 month, centrifuging to remove frozen stock solution, washing cells with PBS, adding the proliferation culture medium, resuspending, and adjusting cell density to 1 × 10 4 seed/mL, in 6-well plates at 37 ℃,5% CO 2 The culture was carried out in the incubator of (1), with changing the medium every 3 days, and continuously for 7 days.
Example 3
The proliferation culture medium for umbilical cord mesenchymal stem cells consists of a DMEM low-sugar culture medium and an additive, wherein the additive consists of: eicosapentaenoic acid, piperine, retinervus Luffae fructus powder, glutathione, sodium selenite, epidermal growth factor, and vitamin C; the mass concentration of each component in the additive in the culture medium is as follows: 6.5ng/mL of eicosapentaenoic acid, 7.4ng/mL of piperine, 9.5ng/mL of loofah sponge powder with the fineness of 100-150 meshes, 15 mu g/mL of glutathione, 10.5ng/mL of sodium selenite, 8ng/mL of epidermal growth factor and 4 mu g/mL of vitamin C.
A culture method of umbilical cord mesenchymal stem cells comprises the following processes: rapidly recovering P3 generation umbilical cord mesenchymal stem cells in logarithmic growth phase, which are frozen for 1 month, in a water bath at 37 ℃, centrifuging to remove frozen stock solution, washing cells with PBS, adding the proliferation culture medium for resuspension, and adjusting the cell density to 5 × 10 4 seed/mL in 12-well plates at 37 ℃,5% CO 2 The culture was carried out in the incubator of (2), the medium was changed every 3 days, and the culture was continued for 7 days.
Comparative example 1
Comparative example 1 provides an umbilical cord mesenchymal stem cell proliferation medium, which is different from example 1 in that: piperine was omitted and the procedure in example 1 was repeated.
Comparative example 2
Comparative example 2 provides an umbilical cord mesenchymal stem cell proliferation medium, which is different from example 1 in that: piperine was omitted and the amount of eicosapentaenoic acid used was adjusted to 11.4ng/mL, the remainder being the same as in example 1.
Comparative example 3
Comparative example 3 provides an umbilical cord mesenchymal stem cell proliferation medium, which is different from example 1 in that: eicosapentaenoic acid was omitted and the rest was the same as in example 1.
Comparative example 4
Comparative example 4 provides an umbilical cord mesenchymal stem cell proliferation medium, which is different from example 1 in that: eicosapentaenoic acid was omitted, and the amount of piperine used was adjusted to 11.4ng/mL, and the remainder was the same as in example 1.
Comparative example 5
Comparative example 5 provides a proliferation medium for umbilical cord mesenchymal stem cells, which is different from example 1 in that: the loofah sponge powder is omitted and the rest is the same as in example 1.
Detecting the OD value from the next day after the umbilical cord mesenchymal stem cells are inoculated, wherein the detection method comprises the following steps: adding 100 μ L CCK-8 into each well, culturing for 2h, detecting light absorption value (OD value) at 450nm with microplate reader, taking average value of 6 wells each time, continuously detecting to 7d, and drawing cell growth curve, wherein the result is shown in FIG. 1. The cells cultured to 7d were sampled, stained with 0.4% trypan blue, and the cell viability was calculated, and the results are shown in table 1.
TABLE 1
| Group of | Example 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 |
| Cell viability (%) | 99.06 | 91.47 | 92.35 | 94.27 | 89.42 | 87.63 |
As can be seen from FIG. 1, the proliferation rate of the umbilical cord mesenchymal stem cells cultured in example 1 is higher, which is significantly higher than that of comparative examples 1 to 5. The composition of the medium was adjusted in comparative examples 1 to 5. In comparative example 1, piperine was omitted and the growth rate of the cells was less decreased than in example 1 as can be seen from the cell proliferation curve. In comparative example 2 in which the amount of eicosapentaenoic acid was increased after omitting piperin, it can be seen from the proliferation curve of the cells that the proliferation rate of umbilical cord mesenchymal stem cells in comparative example 2 was higher than that in comparative example 1, but it was still inferior to that in example 1. In comparative example 3, eicosapentaenoic acid was omitted, and in comparative example 4, the amount of piperine was increased after eicosapentaenoic acid was omitted, and it is understood from fig. 1 that the proliferation rate of umbilical cord mesenchymal stem cells was not as high as that of example 1. The synergistic effect of the eicosapentaenoic acid and the piperine added into the culture medium of the invention is demonstrated to promote the proliferation of the umbilical cord mesenchymal stem cells. Comparative example 5 omits loofah sponge powder, and also influences the proliferation rate of umbilical cord mesenchymal stem cells to a certain extent, which shows that the addition of loofah sponge powder further promotes the proliferation of umbilical cord mesenchymal stem cells, increases the proliferation rate of cells, and a large amount of cells can be harvested through in vitro culture in a short time.
It can be seen from table 1 that the viability of the umbilical cord mesenchymal stem cells is high in example 1, the viability of the cells is decreased in various degrees in comparative examples 1 to 5, and the composition of the culture medium is adjusted in comparative examples 1 to 5. The result shows that the eicosapentaenoic acid, the piperine and the loofah sponge powder in the culture medium are also beneficial to the maintenance of the activity of the umbilical cord mesenchymal stem cells and the reduction of the apoptosis of the umbilical cord mesenchymal stem cells.
The attachment time of umbilical cord mesenchymal stem cells in statistical examples 1 to 3 and comparative examples 1 to 5, respectively, was observed under an inverted microscope, and the results are shown in table 2.
TABLE 2
As can be seen from Table 2, the cell attachment times in examples 1 to 3 were 48 to 49h, and those in comparative examples 1 to 4 were 50 to 51h, which were not much different from example 1. The attachment time in comparative example 5 was 67 hours, and the cells were used longer to attach compared to example 1. In comparative examples 1 to 4, eicosapentaenoic acid, piperine and the amount thereof are adjusted, and in comparative example 5, loofah sponge powder is omitted, which indicates that the loofah sponge powder has a large influence on the adherence performance of umbilical cord mesenchymal stem cells, and the addition of loofah sponge powder helps to shorten the adherence time of umbilical cord mesenchymal stem cells and improve the proliferation characteristics of cells.
The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention should not be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are intended to be covered by the claims.
Claims (7)
1. The proliferation culture medium for umbilical cord mesenchymal stem cells is characterized by comprising a basic culture medium and an additive, wherein the additive comprises the following components: eicosapentaenoic acid, piperine, retinervus Luffae fructus powder, glutathione, sodium selenite, epidermal growth factor, and vitamin C.
2. The umbilical cord mesenchymal stem cell proliferation medium of claim 1, wherein the basal medium is a DMEM low-sugar medium, and the mass concentration of each component in the additive in the medium is as follows: 3.5-6.5ng/mL of eicosapentaenoic acid, 4.2-7.4ng/mL of piperine, 6.5-9.5ng/mL of loofah sponge powder, 10-15 mu g/mL of glutathione, 8.6-10.5ng/mL of sodium selenite, 5-8ng/mL of epidermal growth factor and 1-4 mu g/mL of vitamin C.
3. The umbilical cord mesenchymal stem cell proliferation medium of claim 2, wherein the mass concentration of each component in the additive in the medium is: 5ng/mL of eicosapentaenoic acid, 6.4ng/mL of piperine, 8.5ng/mL of loofah sponge powder, 12 mu g/mL of glutathione, 9.2ng/mL of sodium selenite, 6ng/mL of epidermal growth factor and 3 mu g/mL of vitamin C.
4. The umbilical cord mesenchymal stem cell proliferation medium of claim 2, wherein the fineness of the retinervus luffae fructus powder is 100-150 mesh.
5. A method for culturing umbilical cord mesenchymal stem cells, which comprises culturing the umbilical cord mesenchymal stem cells in the proliferation medium according to any one of claims 1 to 4.
6. The method for culturing umbilical cord mesenchymal stem cells according to claim 5, wherein the umbilical cord mesenchymal stem cells are seeded in the proliferation medium at a density of 1-5 x 10 4 One per mL.
7. The method for culturing umbilical cord mesenchymal stem cells according to claim 5, wherein the percentage of CO is 5% at 37 ℃% 2 The cell culture is performed under conditions.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105705633A (en) * | 2013-05-09 | 2016-06-22 | R生物有限公司 | Culture medium composition for improving regenerative capacity of stem cells, and stem cell culturing method using same |
| CN106190964A (en) * | 2016-07-13 | 2016-12-07 | 中国科学院广州生物医药与健康研究院 | A kind of mesenchymal stem cell serum-free culture medium |
| CN110257328A (en) * | 2019-08-14 | 2019-09-20 | 广州赛莱拉干细胞科技股份有限公司 | A kind of mesenchymal stem cell serum-free culture medium |
| US20190359942A1 (en) * | 2018-05-25 | 2019-11-28 | Jung-Chan Ra | Method for culturing mesenchymal stem cells using gamma-irradiated serum |
| CN110734893A (en) * | 2019-11-15 | 2020-01-31 | 华辰未来(北京)生物医学技术有限公司 | Culture medium containing vitamin C and used for promoting proliferation of umbilical cord mesenchymal stem cells |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105705633A (en) * | 2013-05-09 | 2016-06-22 | R生物有限公司 | Culture medium composition for improving regenerative capacity of stem cells, and stem cell culturing method using same |
| CN106190964A (en) * | 2016-07-13 | 2016-12-07 | 中国科学院广州生物医药与健康研究院 | A kind of mesenchymal stem cell serum-free culture medium |
| US20190359942A1 (en) * | 2018-05-25 | 2019-11-28 | Jung-Chan Ra | Method for culturing mesenchymal stem cells using gamma-irradiated serum |
| CN110257328A (en) * | 2019-08-14 | 2019-09-20 | 广州赛莱拉干细胞科技股份有限公司 | A kind of mesenchymal stem cell serum-free culture medium |
| CN110734893A (en) * | 2019-11-15 | 2020-01-31 | 华辰未来(北京)生物医学技术有限公司 | Culture medium containing vitamin C and used for promoting proliferation of umbilical cord mesenchymal stem cells |
Non-Patent Citations (2)
| Title |
|---|
| TERESA ESPOSITO等: "Effects of curcumin and its adjuvant on TPC1 thyroid cell line" * |
| 艾小林等: "丝瓜络纤维的综合利用研究进展" * |
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