CN115428949B - Probiotic traditional Chinese medicine composition with weight-losing function and preparation method thereof - Google Patents

Abstract

The invention discloses a probiotic traditional Chinese medicine composition with a weight-losing function and a preparation method thereof, wherein the probiotic traditional Chinese medicine composition comprises the following components in percentage by mass: 20-40% of fructo-oligosaccharide; 5-15% of resistant dextrin; 2-15% of white kidney bean extract; 5-20% of fungus powder; 5-15% of erythritol; 5-15% of barley seedling powder; 10-30% of lotus leaf extract; wherein the bacterial powder comprises lactobacillus plantarum P9, lactobacillus casei Zhang and bifidobacterium infantis B1398. The probiotic traditional Chinese medicine composition provided by the invention can obviously inhibit weight gain induced by high-fat diet, simultaneously reduce subcutaneous fat and visceral fat weight, obviously increase basal metabolic rate and energy consumption of an organism, activate thermogenesis function of brown fat, obviously improve glucose tolerance of the organism and enhance insulin sensitivity of peripheral organs.

Description

Probiotic traditional Chinese medicine composition with weight-losing function and preparation method thereof

Technical Field

The invention relates to the field of nutritional health-care foods, in particular to a probiotic traditional Chinese medicine composition with a weight-losing function and a preparation method thereof.

Background

Obesity is a chronic epidemic disease in which fat accumulates in the body due to multiple factors, thereby affecting human health. Obesity has been formally defined by the world health organization (world health organization, WHO) as a chronic metabolic disease as early as 1997, and is typically characterized by increased adipose tissue and weight gain, and is currently measured primarily by the ratio of body weight to height, the Body Mass Index (BMI). With the development and progress of global economy, people's living standard and daily diet have been greatly improved and raised, which has also led to the development of explosive fashion trends of obesity worldwide.

Obesity is an increase in fat deposition due to a long-term imbalance in energy metabolism, i.e., excessive energy intake and/or insufficient energy expenditure. In this state, the occurrence of pathological conditions such as hyperinsulinemia, abnormal blood lipid levels, insulin resistance and the like is often accompanied, and the most important factor is the occurrence of a series of metabolic disorder symptoms such as fatty liver, atherosclerosis, type 2 diabetes, hypertension, hyperlipidemia, cardiovascular diseases, cancers and the like.

There is no effective solution to the problems of obesity caused by long-term imbalance of energy metabolism, and a series of metabolic disorders. At present, the weight losing method mainly solves the problems of weight losing through weight losing, but the weight losing method mainly comprises the steps of exercise weight losing and medicine weight losing, the time required by the exercise weight losing is long, the effect is not clear, and the exercise weight losing method is not easy for most people to adhere to, and the medicine weight losing effect is obvious, but the long-term taking of the medicine weight losing method affects the health of a human body, and the medicine is easy to rebound after stopping taking the medicine.

Disclosure of Invention

Aiming at the prior art, the invention provides a probiotic traditional Chinese medicine composition with a weight-losing function and a preparation method thereof, so as to provide a solution for effectively solving the problems of obesity and metabolic disorder.

A probiotic traditional Chinese medicine composition with a weight-losing function comprises the following raw materials in percentage by mass:

20-40% of fructo-oligosaccharide;

5-15% of resistant dextrin;

2-15% of white kidney bean extract;

5-20% of fungus powder;

5-15% of erythritol;

5-15% of barley seedling powder;

10-30% of lotus leaf extract;

wherein the bacterial powder comprises lactobacillus plantarum P9, lactobacillus casei Zhang and bifidobacterium infantis B1398.

The lactobacillus casei Zhang (Lactobacillus casei Zhang) and lactobacillus plantarum P9 (Lactobacillus plantarum P) are both deposited with the China general microbiological culture Collection center (national patent office designation patent microbiological collection center), address: beijing, china. Wherein the microorganism preservation number of lactobacillus casei Zhang is CGMCC No.5469, and the preservation date is 11 months and 18 days in 2011; the preservation number of the lactobacillus plantarum P9 is CGMCC No.16662, and the preservation time is 2018, 6 and 22 days; bifidobacterium infantis B1398 was deposited under accession number 22241 for 2021, 4 and 27 days.

The lactobacillus casei Zhang (Lactobacillus casei Zhang) is 1 strain of probiotics separated from an inner Mongolian tin Lin Guole big grassland fermented mare milk sample, and has the functions of regulating fatty acid metabolism, resisting oxidization, antagonizing growth of intestinal pathogenic bacteria and the like.

The lactobacillus plantarum P9 (Lactobacillus plantarum P9) is 1 strain of probiotics separated from the inner Mongolian Barbar through natural fermentation of porridge, and has the functions of regulating intestinal flora, reducing in-vivo inflammation, anaphylactic reaction and the like.

The bifidobacterium infantis B1398 (Bifidobacterium infantis B1398) is 1 strain of probiotics obtained from intestinal tracts of healthy infants in pasture area of Qinghai Hainan, and has the functions of adjusting intestinal flora, resisting inflammation, reducing blood fat and the like.

Further, in the probiotic traditional Chinese medicine composition, the mass fraction of the lactobacillus plantarum P9 is 3-10%, the mass fraction of the lactobacillus casei Zhang is 1-5%, and the mass fraction of the bifidobacterium infantis B1398 is 1-5%.

Further, the probiotic traditional Chinese medicine composition comprises the following components in percentage by mass:

30% of fructo-oligosaccharide, 7.5% of resistant dextrin, 10% of white kidney bean extract, 12.5% of fungus powder, 10% of erythritol, 10% of barley seedling powder and 20% of lotus leaf extract.

Further, in the probiotic traditional Chinese medicine composition, the mass of the lactobacillus plantarum P9 in the bacterial powder is 7.5% of that of the probiotic traditional Chinese medicine composition raw material, the mass of the lactobacillus casei Zhang is 2.5% of that of the probiotic traditional Chinese medicine composition raw material, and the mass of the bifidobacterium infantis B1398 is 2.5% of that of the probiotic traditional Chinese medicine composition raw material.

The invention also provides a preparation method of the probiotic traditional Chinese medicine composition with the weight-losing function, which comprises the following steps:

premixing fructo-oligosaccharide, lactobacillus plantarum P9, bifidobacterium infantis B1398 and lactobacillus casei Zhang, and uniformly mixing to obtain premixed powder, wherein the rotating speed of a premixed motor is 10-15 r/min, and the premixing time is 8-15 min;

mixing the premixed powder with the resistant dextrin, the white kidney bean extract, the erythritol, the barley seedling powder and the lotus leaf extract, and uniformly mixing to obtain total mixed powder which is the probiotic traditional Chinese medicine composition, wherein the total mixed motor rotating speed is 10-15 r/min, the mixing time is 30-50 min, and the moisture index in the total mixed powder is controlled to be less than or equal to 5%.

Further, the preparation method comprises the following steps of:

taking qualified lotus leaf decoction pieces, adding 8-12 times of water into the decoction pieces, soaking for 20-40 minutes, heating to boiling, decocting for 1-2 hours, filtering an extracting solution by a 80-mesh filter screen, adding 7-9 times of water into the decoction pieces, heating to boiling, decocting for 50-70 minutes, filtering the extracting solution by a 80-mesh filter screen, merging filtrate, and transferring to a liquid storage tank;

cooling filtrate in the liquid storage tank through a heat exchanger, centrifuging with a disc type centrifuge, controlling a proper liquid inlet rate, and transferring the centrifugate to a concentration tank;

weighing maltodextrin, and adding the weighed maltodextrin into warm water for dissolution;

adding the completely dissolved maltodextrin solution into a concentration tank to be mixed with centrifugate;

concentrating the liquid in the shrinking tank under reduced pressure, controlling the vacuum degree to be between-0.08 and-0.04 MPa, controlling the temperature to be between 70 ℃ and 80 ℃, controlling the steam pressure to be between 0.04 and 0.1MPa, concentrating to prepare concentrated solution with the relative density of 1.08 to 1.10g/ml, passing the concentrated solution through a 80-mesh filter screen, putting the concentrated solution into a clean barrel, and weighing;

and uniformly stirring the concentrated solution, and then carrying out spray drying.

Further, the mass of maltodextrin in the preparation method is 6% of the total amount of lotus leaf decoction pieces.

The probiotic traditional Chinese medicine composition has the following advantages:

can significantly inhibit weight gain induced by high fat diet while reducing subcutaneous fat and visceral fat weight;

the basal metabolic rate and the energy consumption of the organism can be obviously increased;

can activate the thermogenic function of brown fat; can significantly improve glucose tolerance of the body and enhance insulin sensitivity of peripheral organs;

can remarkably improve brown fat activity and promote white fat beige.

Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.

Drawings

Fig. 1A is a schematic representation of the effect of a probiotic traditional Chinese medicine composition on inhibiting the weight induced by a high fat diet in mice with p <0.001;

fig. 1B shows that mice SUB (subcutaneous fat, ×p < 0.05) and EP (visceral fat, ×p < 0.01) in the probiotic chinese medicinal composition treated group were significantly reduced compared to the control group, but the BAT group was not significantly changed;

fig. 1C is a schematic diagram of the effect of a probiotic chinese herbal composition on glucose tolerance in HFD mice, p <0.01;

fig. 1D is a schematic representation of the effect of probiotic chinese herbal composition on insulin sensitivity in HFD mice, p <0.05;

FIG. 1E is a schematic representation of the amount of exercise in mice between treatment groups;

fig. 1F is a schematic diagram of the effect of probiotic chinese herbal composition on energy expenditure in HFD mice, p <0.05;

FIG. 1G is a graph showing the temperature change of mice treated with the probiotic Chinese medicinal composition after cold stimulation,

*p<0.05；

FIG. 1H is a schematic illustration of the effect of probiotic traditional Chinese medicine composition treatment on body surface temperature of HFD mice;

FIG. 2A is a representation of immunohistochemical staining of BAT and SUB tissue UCP1 in mice treated with a probiotic Chinese medicinal composition;

fig. 2B is an upregulation of COX5 expression in both BAT (< p < 0.01) and SUB (< p < 0.05) tissues in mice treated with probiotic chinese herbal compositions;

fig. 2C shows that the probiotic chinese medicinal composition significantly upregulates thermogenesis-related proteins and mitochondrial oxidative phosphorylation-related proteins in BAT and SUB tissues.

Detailed Description

The following detailed description of the present invention will provide further understanding of the objects, features and advantages of the present invention by way of example. Several embodiments of the invention are presented in the examples. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Example 1

A probiotic Chinese medicinal composition with weight reducing function comprises (by weight) fructo-oligosaccharide 30%, resistant dextrin 7.5%, white kidney bean extract 10%, fungus powder 12.5%, erythritol 10%, barley seedling powder 10% and folium Nelumbinis extract 20%.

The bacterial powder consists of 7.5% of lactobacillus plantarum, 2.5% of lactobacillus casei Zhang and 2.5% of bifidobacterium infantis B1398.

The preparation method of the probiotic traditional Chinese medicine composition comprises the following steps:

premixing fructo-oligosaccharide, lactobacillus plantarum P9, bifidobacterium infantis B1398 and lactobacillus casei Zhang, and uniformly mixing to obtain premixed powder, wherein the rotating speed of a premixed motor is 10-15 r/min, and the premixing time is 8-15 min;

mixing the premixed powder with the resistant dextrin, the white kidney bean extract, the erythritol, the barley seedling powder and the lotus leaf extract, and uniformly mixing to obtain total mixed powder which is the probiotic traditional Chinese medicine composition, wherein the total mixed motor rotating speed is 10-15 r/min, the mixing time is 30-50 min, and the moisture index in the total mixed powder is controlled to be less than or equal to 5%.

Specifically, in the preparation method of the probiotic traditional Chinese medicine composition, the preparation steps of the lotus leaf extract comprise:

(1) Taking qualified lotus leaf decoction pieces, adding 8-12 times of water into the decoction pieces, soaking for 20-40 minutes, heating to boiling, decocting for 1-2 hours, filtering an extracting solution by a 80-mesh filter screen, adding 7-9 times of water into the decoction pieces, heating to boiling, decocting for 50-70 minutes, filtering the extracting solution by a 80-mesh filter screen, merging filtrate, and transferring to a liquid storage tank;

(2) Cooling filtrate in the liquid storage tank through a heat exchanger, centrifuging with a disc type centrifuge, controlling a proper liquid inlet rate, and transferring the centrifugate to a concentration tank;

(3) Weighing maltodextrin, and adding the weighed maltodextrin into warm water for dissolution;

adding the completely dissolved maltodextrin solution into a concentration tank to be mixed with centrifugate;

(4) Concentrating the liquid in the shrinking tank under reduced pressure, controlling the vacuum degree to be between-0.08 and-0.04 MPa, controlling the temperature to be between 70 ℃ and 80 ℃, controlling the steam pressure to be between 0.04 and 0.1MPa, concentrating to prepare concentrated solution with the relative density of 1.08 to 1.10g/ml, passing the concentrated solution through a 80-mesh filter screen, putting the concentrated solution into a clean barrel, and weighing;

(5) And uniformly stirring the concentrated solution, and then carrying out spray drying.

Specifically, the mass of maltodextrin in the step (3) is 6% of the total amount of lotus leaf decoction pieces.

Example 2

A probiotic Chinese medicinal composition with weight reducing function comprises, by mass, fructo-oligosaccharide 20%, resistant dextrin 5.5%, white kidney bean extract 15%, lactobacillus plantarum P9.5%, lactobacillus casei Zhang 1.5%, bifidobacterium infantis B1398 1%, erythritol 10%, barley seedling powder 5%, and folium Nelumbinis extract 30%.

Example 3

A probiotic Chinese medicinal composition with weight reducing function comprises, by mass, 20% of fructo-oligosaccharide, 5% of resistant dextrin, 2% of white kidney bean extract, 9% of lactobacillus plantarum P9%, 5% of lactobacillus casei Zhang and 10% of bifidobacterium infantis B1398%, 10% of erythritol, 15% of barley seedling powder and 10% of lotus leaf extract.

The probiotic Chinese medicinal composition obtained in example 1 above was subjected to a number of experiments with 4-6 week old adult male C57BL/6 mice to investigate the effect of the probiotic Chinese medicinal composition on the metabolic level of HFD (high fat diet) mice, and to investigate the effect of the probiotic Chinese medicinal composition on the brown fat activity and white fat beige of HFD mice. All mice used for experiments were kept in the SPF-grade animal management center of the institute of Chinese sciences, five mice per cage were fed 60% kcal high-fat diet and water, the environment was maintained at a constant temperature of 24℃and 12h of light per day was ensured. The probiotic Chinese medicinal composition is administered in a manner of gastric lavage at 300 mg/Kg. The following describes the study and experimental conditions in detail.

Study 1, effect of probiotic Chinese medicinal composition on HFD (high fat diet) mice metabolism level

In order to explore the therapeutic effect of the probiotic Chinese medicinal composition on obesity and metabolic diseases, after an obesity model (HFD) is constructed by feeding 60% Kcal High-fat feed to mice, 300mg/Kg of the probiotic Chinese medicinal composition is subjected to gastric lavage treatment to evaluate the effect of the probiotic Chinese medicinal composition on the metabolic level of the mice.

The change of body weight of HFD mice after the stomach is irrigated with the probiotic Chinese medicinal composition was continuously recorded for 10 weeks. As a result, as shown in fig. 1A, the probiotic traditional Chinese medicine composition can significantly inhibit weight gain of mice induced by a high-fat diet at week 10 (< p < 0.001). At the same time, mice were weighed at week 10 for brown fat (Brown adipose tissue, BAT), subcutaneous white fat (Subcutaneous white adipose tissue, SUB) and visceral fat (Epididymal adipose tissue, EP), and SUB (< 0.05) and EP (< 0.01) of the probiotic chinese herbal composition treated group mice were also significantly reduced at the same time with a decrease in weight gain, while the weight of brown fat was not significantly changed (fig. 1B). This indicates that the probiotic traditional Chinese medicine composition has the effect of inhibiting the weight increase of mice induced by high-fat diet.

Fig. 1A shows that the probiotic Chinese medicinal composition (shown in fig. 1A) can significantly inhibit weight gain of mice induced by a high-fat diet, wherein the HFD model group (shown in fig. 1A) is red, and p <0.001 is the probiotic Chinese medicinal composition treatment group. Fig. 1B shows that mice SUB (subcutaneous fat, ×p < 0.05) and EP (visceral fat, ×p < 0.01) in the probiotic chinese medicinal composition treated group were significantly reduced compared to the control group (HFD in fig. 1A), but the BAT group was not significantly altered.

Moreover, through researches, the utilization of blood sugar and insulin sensitivity of peripheral organs can be improved while the activity of brown fat is improved, so that the effects of treating obesity, type 2 diabetes and other related metabolic diseases are achieved. To further determine the interaction of the probiotic traditional Chinese medicine composition with glucose tolerance and insulin sensitivity in HFD mice, glucose tolerance experiments (Glucose Tolerance Test, GTT) and insulin tolerance experiments (Insulin Tolerance Test, ITT) were performed on HFD mice, respectively. As a result, as shown in fig. 1C, the glucose tolerance of HFD mice in the probiotic traditional Chinese medicine composition treatment group was significantly enhanced (p < 0.01). At the same time, the insulin sensitivity of the mice of the experimental group was also significantly improved (< p < 0.05) (fig. 1D). This shows that the probiotic traditional Chinese medicine composition can significantly improve glucose tolerance of HFD mice and enhance insulin sensitivity of peripheral organs.

Obesity occurs mostly due to the intake of energy greater than the consumption of energy. Next, in order to verify that the inhibition of HFD mice by the probiotic chinese herbal composition is caused by the increase of energy consumption of the mice organism by the probiotic chinese herbal composition, an energy consumption experiment was performed on HFD mice. As shown in fig. 1E and F, the oxygen consumption of mice of the probiotic chinese herbal composition group was significantly increased (p < 0.05) in both the daytime rest and the nighttime active periods without significant change in exercise amount.

Since brown fat is an important thermogenic organ of the body, it can maintain a constant body temperature by non-shivering thermogenesis under cold stimulation. In order to verify whether the activity of brown fat is improved by activating the brown fat or not, the purpose of increasing energy consumption is achieved by the traditional Chinese medicine composition for growing bacteria. For this reason, HFD mice were subjected to cold stimulation experiments. After 4h cold stimulation of the experimental mice with a 4 ℃ incubator, the rectal temperature was measured and found that the body temperature of the mice in the probiotic traditional Chinese medicine composition treatment group was significantly higher than that of the control group (p < 0.05) (fig. 1G). Simultaneously, the experimental mice were subjected to infrared scanning imaging. As shown in fig. 1H, the probiotic traditional Chinese medicine composition can significantly increase the body surface temperature of mice. These results indicate that the probiotic traditional Chinese medicine composition can significantly increase the activity of brown fat of mice and the thermogenic function.

Study 2, study of probiotic Chinese medicinal composition on increasing Brown fat Activity and promoting white fat beige in HFD mice

Several studies have shown that white fat treated with β3-adrenoreceptor agonists or PPAR-gamma agonists may appear as part of brown adipose-like cells, i.e. beige fat. And classical brown adipose-like cells and beige adipocytes are present simultaneously in healthy adult humans. In order to further investigate whether the probiotic traditional Chinese medicine composition can promote white fat browning of the HFD mice, western blotting experiments, namely UCP1 immunohistochemical staining, are carried out on BAT and SUB tissues of experimental mice, and as shown in a figure 2A, lipid droplets in the BAT and SUB tissues of the HFD mice treated by the probiotic traditional Chinese medicine composition are obviously reduced, and white fat beige phenomenon occurs. Simultaneously, qPCR results showed a significant increase in COX5 expression in BAT (p < 0.01) and SUB (p < 0.05) tissues according to RNA extraction and real-time quantitative PCR experiments (fig. 2B). In addition, western blot detection shows that both the thermogenesis-related proteins and mitochondrial oxidative phosphorylation-related proteins in BAT and SUB tissues are significantly up-regulated (fig. 2C). This suggests that the probiotic Chinese medicinal composition improves obesity by increasing energy consumption of the body and promotes beige white fat.

The respective experiments involved in the above two studies are specifically described below.

1. Experiment of energy consumption

The energy consumption test was used to measure the metabolic rate and the exercise amount, and the metabolic rate in the test was evaluated by oxygen consumption. When oxygen consumption is measured, the indoor environment is consistent with the feeding conditions of the SPF-class animal house, and the constant temperature of 24 ℃ and 12h illumination are maintained. Mice fed 3 weeks on high fat diet were placed individually in TSE metabolic cages, and after 24h adaptation, the levels of oxygen and carbon dioxide in the cages were recorded for the next 24 h. When the amount of exercise was measured, the mice were individually placed in a Columbus system equipped cage, and the amount of exercise within 24 hours of the mice was calculated by the light beam technique.

2. Experiment of Cold stimulation

In this experiment, after the mice were placed in a 4 ℃ incubator for stimulation for 4 hours, rectal temperatures before and after cold stimulation of the mice were measured with an anal thermometer. During the temperature measurement, it is necessary to ensure a consistent length each time it goes deep into the rectum. The skin temperature around the brown fat before and after cold stimulation was recorded simultaneously using an infrared imager and data analysis was performed using FLIR software.

3. Glucose tolerance test

Mice were fasted for 16h before the start of the experiment and were numbered. Blood was taken from the rat tail, and fasting blood glucose was measured with a blood glucose meter. After the mice had been acclimatized for 30min, 2.0g/Kg glucose solution was intraperitoneally injected, and timing was started after the completion of the injection, and blood glucose values were recorded at 15, 30, 60, 90 and 120 minutes after the injection, respectively.

4. Insulin resistance test

After 4h fasted mice before the start of the experiment, blood was taken from the rat tail and fasting blood glucose values were recorded. After the mice had been acclimatized for 30min, 0.6U/Kg of insulin solution was intraperitoneally injected, and timing was started after the completion of the injection, and blood glucose values were recorded at 15, 30, 45 and 60 minutes after the injection, respectively.

RNA extraction and real-time quantitative PCR experiments

Total RNA in tissues and cells was extracted by Trizol method, and 2. Mu.g of total RNA was reverse transcribed into cDNA using a high fidelity cDNA reverse transcription kit. qPCR reactions were performed using SYBR Green Master Mix and detected by Prism VIIA7 real-time PCR system. Primers required for qPCR reactions were designed by Primer Quest.

6. Western blotting experiment

The total protein of the tissues and cells was extracted by RIPA lysis, and the RIPA buffer system comprised 150mM sodium chloride, 1.0% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50mM protease-containing Tris and phosphatase inhibitors. An equal amount of protein sample was subjected to 10% SDS-polyacrylamide gel electrophoresis, and then the protein was transferred onto PVDF membrane, incubated with blocking buffer (5% skimmed milk) for 1h at room temperature, and blotted overnight with anti-UCP 1, anti-HSP 90, anti-phosphorus antibodies.

After the end of the primary antibody incubation and washing, PVDF membrane was incubated with HRP-conjugated secondary antibody for 1 hour at room temperature. Chemiluminescent color development was performed using Mini chemi 580 with Super Signal West Pico chemiluminescent substrate, and photographic and image observation and analysis were performed in a GE Healthcare gel imaging system.

Experiments show that the probiotic traditional Chinese medicine composition in the embodiment has the following beneficial effects:

(1) Can significantly inhibit weight gain induced by high fat diet while reducing subcutaneous fat and visceral fat weight;

(2) The basal metabolic rate and the energy consumption of the organism can be obviously increased;

(3) Can activate the thermogenic function of brown fat;

(4) Can significantly improve glucose tolerance of the body and enhance insulin sensitivity of peripheral organs;

(5) Can remarkably improve brown fat activity and promote white fat beige.

It should be noted that, the probiotic traditional Chinese medicine compositions prepared in the embodiment 2 and the third embodiment 3 of the present invention were tested by the same experimental method as in the embodiment 1, and the effects thereof are the same as those of the first embodiment, and can increase the metabolic level of HFD mice, increase the brown fat activity of HFD mice, promote the beige of white fat, and the like.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims (6)

1. The probiotic traditional Chinese medicine composition with the weight-losing function is used for increasing energy consumption by activating brown fat, and is characterized by comprising the following raw materials in percentage by mass:

20-40% of fructo-oligosaccharide;

5-15% of resistant dextrin;

2-15% of white kidney bean extract;

5-20% of fungus powder;

5-15% of erythritol;

5-15% of barley seedling powder;

10-30% of lotus leaf extract;

the probiotic traditional Chinese medicine composition comprises 3-10% of lactobacillus plantarum P9, 1-5% of lactobacillus casei Zhang and 1-5% of bifidobacterium infantis B1398.

2. The probiotic traditional Chinese medicine composition according to claim 1, comprising, in mass percent:

30% of fructo-oligosaccharide, 7.5% of resistant dextrin, 10% of white kidney bean extract, 12.5% of fungus powder, 10% of erythritol, 10% of barley seedling powder and 20% of lotus leaf extract.

3. The probiotic traditional Chinese medicine composition according to claim 2, wherein in the bacterial powder, the mass of lactobacillus plantarum P9 is 7.5% of that of the probiotic traditional Chinese medicine composition raw material, the mass of lactobacillus casei Zhang is 2.5% of that of the probiotic traditional Chinese medicine composition raw material, and the mass of bifidobacterium infantis B1398 is 2.5% of that of the probiotic traditional Chinese medicine composition raw material.

4. A method of preparing a probiotic Chinese medicinal composition according to any one of claims 1 to 3, comprising:

premixing fructo-oligosaccharide, lactobacillus plantarum P9, bifidobacterium infantis B1398 and lactobacillus casei Zhang, and uniformly mixing to obtain premixed powder, wherein the rotating speed of a premixed motor is 10-15 r/min, and the premixing time is 8-15 min;

mixing the premixed powder with the resistant dextrin, the white kidney bean extract, the erythritol, the barley seedling powder and the lotus leaf extract, and uniformly mixing to obtain total mixed powder which is the probiotic traditional Chinese medicine composition, wherein the total mixed motor rotating speed is 10-15 r/min, the mixing time is 30-50 min, and the moisture index in the total mixed powder is controlled to be less than or equal to 5%.

5. The method of preparing as claimed in claim 4, wherein the step of preparing the lotus leaf extract comprises:

taking qualified lotus leaf decoction pieces, adding 8-12 times of water into the decoction pieces, soaking for 20-40 minutes, heating to boiling, decocting for 1-2 hours, filtering an extracting solution by a 80-mesh filter screen, adding 7-9 times of water into the decoction pieces, heating to boiling, decocting for 50-70 minutes, filtering the extracting solution by a 80-mesh filter screen, merging filtrate, and transferring to a liquid storage tank;

cooling filtrate in the liquid storage tank through a heat exchanger, centrifuging with a disc type centrifuge, controlling a proper liquid inlet rate, and transferring the centrifugate to a concentration tank;

weighing maltodextrin, and adding the weighed maltodextrin into warm water for dissolution;

adding the completely dissolved maltodextrin solution into a concentration tank to be mixed with centrifugate;

concentrating the liquid in the shrinking tank under reduced pressure, controlling the vacuum degree to be between-0.08 and-0.04 MPa, controlling the temperature to be between 70 ℃ and 80 ℃, controlling the steam pressure to be between 0.04 and 0.1MPa, concentrating to prepare concentrated solution with the relative density of 1.08 to 1.10g/ml, passing the concentrated solution through a 80-mesh filter screen, putting the concentrated solution into a clean barrel, and weighing;

and uniformly stirring the concentrated solution, and then carrying out spray drying.

6. The preparation method according to claim 5, wherein the mass of maltodextrin is 6% of the total amount of lotus leaf decoction pieces.