CN115406869A - High-sensitivity U-shaped metal super-surface biosensor and use method thereof - Google Patents
High-sensitivity U-shaped metal super-surface biosensor and use method thereof Download PDFInfo
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- CN115406869A CN115406869A CN202211250320.3A CN202211250320A CN115406869A CN 115406869 A CN115406869 A CN 115406869A CN 202211250320 A CN202211250320 A CN 202211250320A CN 115406869 A CN115406869 A CN 115406869A
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- 229910052751 metal Inorganic materials 0.000 title claims abstract description 46
- 239000002184 metal Substances 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 8
- 239000002086 nanomaterial Substances 0.000 claims description 27
- 239000000758 substrate Substances 0.000 claims description 15
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 3
- 239000010931 gold Substances 0.000 claims description 3
- 229910052737 gold Inorganic materials 0.000 claims description 3
- 229910000510 noble metal Inorganic materials 0.000 claims description 3
- 235000012239 silicon dioxide Nutrition 0.000 claims description 3
- 239000000377 silicon dioxide Substances 0.000 claims description 3
- 230000005684 electric field Effects 0.000 claims description 2
- 230000008859 change Effects 0.000 abstract description 13
- 230000035945 sensitivity Effects 0.000 abstract description 12
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 230000003287 optical effect Effects 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 4
- 230000004044 response Effects 0.000 abstract description 3
- 238000013461 design Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000004186 food analysis Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012269 metabolic engineering Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
The invention discloses a high-sensitivity U-shaped metal super-surface biosensor and a using method thereof. According to the invention, the concentration of the solution to be measured is represented by measuring the position change of a resonance peak through a spectrometer by virtue of the characteristic that the magnetic resonance optical response characteristic of the set metal micro-nano array structure excited under vertical incidence has high sensitivity along with the change of the environmental refractive index. The refractive index sensor designed by the invention has the advantages of simple structure, easy processing and high sensing sensitivity, and can be used for realizing the sensing of the unmarked antigen-antibody binding rate.
Description
Technical Field
The invention relates to the technical field of micro-nano optical sensing, in particular to a high-sensitivity U-shaped metal super-surface biosensor and a using method thereof.
Background
In recent years, biosensors have been widely used as analytical tools that take biological reactions as input and convert them into electrical signals. Biosensors are self-contained, integrated devices that provide quantitative results for systematic and comprehensive analysis by biological recognition or receptors in contact with the sensor. Biosensors have high specificity, selectivity, independence from physical limitations such as pH and temperature, and other several advantages, and thus are applied to various fields including environmental assessment, medical diagnosis, metabolic engineering, and food analysis, etc. Among them, the plasma biosensor has extremely high specificity and sensitivity, which makes it have great interest in various fields such as virus detection, diagnosis of pollutants in the environment, monitoring of biomolecules in food, and the like. Due to the nanoscale near-field enhancement with high sensitivity and non-destructive operation, label-free biomolecular sensing based on plasma super-surfaces has achieved considerable success for high-throughput real-time detection. Driven by the strong demand for rapid, highly sensitive and cost-effective biosensing in point-of-care and ambulatory medicine, researchers have improved the performance of plasma sensors by using various nanostructured meta-surfaces, but most of the processing is complex.
Most of the existing biological detection technologies can damage biomolecules, the operation is complex, the plasmon biosensor has the effect similar to an antibody, the resonance position can be changed after the plasmon biosensor is combined with antigens with different concentrations, and the plasmon biosensor has the characteristics of high sensitivity, no need of labeling and the like, so that the design of the plasmon sensor which is low in cost, high in sensitivity, easy to process and capable of being repeatedly manufactured is of great significance.
Disclosure of Invention
The invention aims to provide a high-sensitivity U-shaped metal super-surface biosensor and a using method thereof, which solve the problem that in the prior art, according to the magnetic resonance optical response characteristic of a metal micro-nano array structure excited under vertical incidence, the change of the ambient environment can still be sensitively sensed through the change of a resonance position under the condition of small change of the ambient refractive index, and the sensitivity of a refractive index sensor is greatly enhanced.
The technical scheme adopted by the invention is as follows:
a high-sensitivity U-shaped metal super-surface biosensor comprises a substrate and metal micro-nano structures arranged on the substrate, wherein the metal micro-nano structures are U-shaped, the U-shaped included angle of the metal micro-nano structures is 90 degrees, and the metal micro-nano structures are arrayed periodically along the X direction and the Y direction.
Furthermore, the periods of the metal micro-nano structure in the X direction and the Y direction are both 400nm.
Further, the metal micro-nano structure is noble metal gold.
Further, the length of the U-shaped unit structure of the metal micro-nano structure is 200nm, the distance between the bottoms of the U-shaped unit structures is 80nm, the distance between two arms of the U-shaped unit structure is 100nm, and the height of the U-shaped unit structure is 30nm.
Further, the substrate is made of silicon dioxide, and the refractive index of the substrate is 1.45.
Further, the biosensor adopts a broad spectrum light source to vertically irradiate the emission signal to the surface of the biosensor, and adopts a spectrometer to receive the reflection signal.
Furthermore, the biosensor works in the range of near infrared wavelength of 1.5-2.0 μm.
Furthermore, the detection range of the biosensor to the external environment refractive index is 1.3-1.5.
The invention also provides a use method of the biosensor based on any one of the above, the metal micro-nano structure is positioned on the substrate, the light source is a plane wave, the electric field of the biosensor is polarized along the x axis and vertically incident along the-z axis, the magnetic resonance mode of the metal micro-nano structure is excited, and the refractive index of the biological solution to be measured is represented by the position of a resonance peak displayed by a spectrometer.
The invention has the beneficial effects that: according to the invention, the concentration of the solution to be measured is represented by measuring the position change of a resonance peak through a spectrometer by virtue of the characteristic that the magnetic resonance optical response characteristic of the set metal micro-nano array structure excited under vertical incidence has high sensitivity along with the change of the environmental refractive index. The refractive index sensor designed by the invention has the advantages of simple structure, easy processing and high sensing sensitivity, and can be used for realizing the sensing of the unmarked antigen-antibody binding rate.
Drawings
FIG. 1 is a schematic diagram of a three-dimensional periodic structure of a high-sensitivity U-shaped metal super-surface biosensor according to the present invention;
FIG. 2 is a schematic diagram of a metal micro-nano structure according to the present invention;
FIG. 3 is a graph of the sensitivity function of the sensor fitted to an ambient refractive index in the range of 1.3 to 1.5 in the example.
Description of the reference numerals
1-substrate, 2-metal micro-nano structure.
Detailed Description
The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-2, the high-sensitivity U-shaped metal super-surface biosensor comprises a substrate 1 and metal micro-nano structures 2 arranged on the substrate 1, wherein the metal micro-nano structures 2 are U-shaped, the U-shaped included angle of the metal micro-nano structures 2 is 90 degrees, and the metal micro-nano structures 2 are arranged in a periodic array along the X and Y directions.
The periods of the metal micro-nano structure 2 in the X direction and the Y direction are both 400nm.
The metal micro-nano structure 2 is noble metal gold.
The length a of the U-shaped unit structure of the metal micro-nano structure 2 is 200nm, the distance b between the bottoms of the U-shaped unit structures is 80nm, the distance c between two arms of the U-shaped unit structure is 100nm, and the height h of the U-shaped unit structure is 30nm.
The material of the substrate 1 is silicon dioxide, and the refractive index of the substrate 1 is 1.45.
The biosensor adopts a broad spectrum light source to vertically irradiate an emission signal to the surface of the biosensor, and a spectrometer is adopted to receive a reflection signal.
The range of the working of the biosensor in the near infrared wavelength is 1.5-2.0 μm.
The detection range of the biosensor to the outside environment refractive index is 1.3-1.5.
The embodiment is as follows: dropping a solution to be detected on the surface of the biosensor, vertically approaching an optical fiber to the surface, observing the change of a resonance position through a spectrometer, and obtaining a result as shown in figure 3, wherein when the refractive index of the solution to be detected is increased from 1.3 to 1.5, a red shift phenomenon appears at the resonance position along with the increase of the refractive index, the transmission value does not obviously change along with the change of the refractive index, when the environmental refractive index changes by 0.02, the resonance position moves by at least more than 15nm, the rule of the resonance position along with the change of the refractive index is further fitted, and through calculation, in a refractive index change interval, the linear relation between a resonance wavelength (lambda) and the refractive index (n) is expressed as: λ =728.1+843.1 + n, the refractive index sensitivity S of the designed refractive index sensor in this interval is 843.1nm/RIU.
The invention has simple structural design, easy processing, high sensitivity, no need of marking and simple operation, and the concentration and the sensing performance of the solution to be measured can be obtained by observing the change of the resonance position through the spectrometer.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The high-sensitivity U-shaped metal super-surface biosensor is characterized by comprising a substrate and metal micro-nano structures arranged on the substrate, wherein the metal micro-nano structures are U-shaped, the U-shaped included angle of the metal micro-nano structures is 90 degrees, and the metal micro-nano structures are arrayed periodically along the X direction and the Y direction.
2. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the metal micro-nano structure has a period of 400nm in both X and Y directions.
3. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the metal micro-nano structure is noble metal gold.
4. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the length of the U-shaped unit structure of the metal micro-nano structure is 200nm, the distance between the bottoms of the U-shaped unit structures is 80nm, the distance between the two arms of the U-shaped unit structure is 100nm, and the height of the U-shaped unit structure is 30nm.
5. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the substrate is made of silicon dioxide, and the refractive index of the substrate is 1.45.
6. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the biosensor uses a broad-spectrum light source to vertically incident emission signals on the surface of the biosensor, and uses a spectrometer to receive reflection signals.
7. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the biosensor works in the near infrared wavelength range of 1.5-2.0 μm.
8. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the detection range of the biosensor to the external environment refractive index is 1.3-1.5.
9. The use method of the biosensor based on any one of claims 1 to 8 is characterized in that the metal micro-nano structure is positioned on a substrate, a light source is adopted as a plane wave, an electric field of the biosensor is polarized along an x axis and vertically incident along a-z axis, a magnetic resonance mode of the metal micro-nano structure is excited, and the position of a resonance peak displayed by a spectrometer represents the refractive index of a biological solution to be measured.
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| CN202211250320.3A CN115406869A (en) | 2022-10-13 | 2022-10-13 | High-sensitivity U-shaped metal super-surface biosensor and use method thereof |
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| CN202211250320.3A CN115406869A (en) | 2022-10-13 | 2022-10-13 | High-sensitivity U-shaped metal super-surface biosensor and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116953828A (en) * | 2023-09-12 | 2023-10-27 | 之江实验室 | Multiband absorber and design method thereof |
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|---|---|---|---|---|
| CN106290270A (en) * | 2016-07-22 | 2017-01-04 | 浙江大学 | A kind of Fluorescence Increasing structure based on U-shaped metal array structure and system |
| CN114264627A (en) * | 2021-12-20 | 2022-04-01 | 河南工业大学 | Terahertz sensor and using method thereof |
| CN114858754A (en) * | 2022-04-29 | 2022-08-05 | 聊城大学 | Novel refractive index biosensor and manufacturing method thereof |
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- 2022-10-13 CN CN202211250320.3A patent/CN115406869A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106290270A (en) * | 2016-07-22 | 2017-01-04 | 浙江大学 | A kind of Fluorescence Increasing structure based on U-shaped metal array structure and system |
| CN114264627A (en) * | 2021-12-20 | 2022-04-01 | 河南工业大学 | Terahertz sensor and using method thereof |
| CN114858754A (en) * | 2022-04-29 | 2022-08-05 | 聊城大学 | Novel refractive index biosensor and manufacturing method thereof |
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| 张萌徕 等: "基于开口环阵列结构的表面晶格共振产生及二次谐波增强" * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116953828A (en) * | 2023-09-12 | 2023-10-27 | 之江实验室 | Multiband absorber and design method thereof |
| CN116953828B (en) * | 2023-09-12 | 2024-03-12 | 之江实验室 | Multiband absorber and design method thereof |
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Application publication date: 20221129 |