CN115406869A - High-sensitivity U-shaped metal super-surface biosensor and use method thereof - Google Patents

High-sensitivity U-shaped metal super-surface biosensor and use method thereof Download PDF

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Publication number
CN115406869A
CN115406869A CN202211250320.3A CN202211250320A CN115406869A CN 115406869 A CN115406869 A CN 115406869A CN 202211250320 A CN202211250320 A CN 202211250320A CN 115406869 A CN115406869 A CN 115406869A
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biosensor
sensitivity
shaped
metal
metal micro
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张萌徕
张磊
王敬好
储涛
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Zhejiang Lab
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Zhejiang Lab
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited

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Abstract

The invention discloses a high-sensitivity U-shaped metal super-surface biosensor and a using method thereof. According to the invention, the concentration of the solution to be measured is represented by measuring the position change of a resonance peak through a spectrometer by virtue of the characteristic that the magnetic resonance optical response characteristic of the set metal micro-nano array structure excited under vertical incidence has high sensitivity along with the change of the environmental refractive index. The refractive index sensor designed by the invention has the advantages of simple structure, easy processing and high sensing sensitivity, and can be used for realizing the sensing of the unmarked antigen-antibody binding rate.

Description

High-sensitivity U-shaped metal super-surface biosensor and use method thereof
Technical Field
The invention relates to the technical field of micro-nano optical sensing, in particular to a high-sensitivity U-shaped metal super-surface biosensor and a using method thereof.
Background
In recent years, biosensors have been widely used as analytical tools that take biological reactions as input and convert them into electrical signals. Biosensors are self-contained, integrated devices that provide quantitative results for systematic and comprehensive analysis by biological recognition or receptors in contact with the sensor. Biosensors have high specificity, selectivity, independence from physical limitations such as pH and temperature, and other several advantages, and thus are applied to various fields including environmental assessment, medical diagnosis, metabolic engineering, and food analysis, etc. Among them, the plasma biosensor has extremely high specificity and sensitivity, which makes it have great interest in various fields such as virus detection, diagnosis of pollutants in the environment, monitoring of biomolecules in food, and the like. Due to the nanoscale near-field enhancement with high sensitivity and non-destructive operation, label-free biomolecular sensing based on plasma super-surfaces has achieved considerable success for high-throughput real-time detection. Driven by the strong demand for rapid, highly sensitive and cost-effective biosensing in point-of-care and ambulatory medicine, researchers have improved the performance of plasma sensors by using various nanostructured meta-surfaces, but most of the processing is complex.
Most of the existing biological detection technologies can damage biomolecules, the operation is complex, the plasmon biosensor has the effect similar to an antibody, the resonance position can be changed after the plasmon biosensor is combined with antigens with different concentrations, and the plasmon biosensor has the characteristics of high sensitivity, no need of labeling and the like, so that the design of the plasmon sensor which is low in cost, high in sensitivity, easy to process and capable of being repeatedly manufactured is of great significance.
Disclosure of Invention
The invention aims to provide a high-sensitivity U-shaped metal super-surface biosensor and a using method thereof, which solve the problem that in the prior art, according to the magnetic resonance optical response characteristic of a metal micro-nano array structure excited under vertical incidence, the change of the ambient environment can still be sensitively sensed through the change of a resonance position under the condition of small change of the ambient refractive index, and the sensitivity of a refractive index sensor is greatly enhanced.
The technical scheme adopted by the invention is as follows:
a high-sensitivity U-shaped metal super-surface biosensor comprises a substrate and metal micro-nano structures arranged on the substrate, wherein the metal micro-nano structures are U-shaped, the U-shaped included angle of the metal micro-nano structures is 90 degrees, and the metal micro-nano structures are arrayed periodically along the X direction and the Y direction.
Furthermore, the periods of the metal micro-nano structure in the X direction and the Y direction are both 400nm.
Further, the metal micro-nano structure is noble metal gold.
Further, the length of the U-shaped unit structure of the metal micro-nano structure is 200nm, the distance between the bottoms of the U-shaped unit structures is 80nm, the distance between two arms of the U-shaped unit structure is 100nm, and the height of the U-shaped unit structure is 30nm.
Further, the substrate is made of silicon dioxide, and the refractive index of the substrate is 1.45.
Further, the biosensor adopts a broad spectrum light source to vertically irradiate the emission signal to the surface of the biosensor, and adopts a spectrometer to receive the reflection signal.
Furthermore, the biosensor works in the range of near infrared wavelength of 1.5-2.0 μm.
Furthermore, the detection range of the biosensor to the external environment refractive index is 1.3-1.5.
The invention also provides a use method of the biosensor based on any one of the above, the metal micro-nano structure is positioned on the substrate, the light source is a plane wave, the electric field of the biosensor is polarized along the x axis and vertically incident along the-z axis, the magnetic resonance mode of the metal micro-nano structure is excited, and the refractive index of the biological solution to be measured is represented by the position of a resonance peak displayed by a spectrometer.
The invention has the beneficial effects that: according to the invention, the concentration of the solution to be measured is represented by measuring the position change of a resonance peak through a spectrometer by virtue of the characteristic that the magnetic resonance optical response characteristic of the set metal micro-nano array structure excited under vertical incidence has high sensitivity along with the change of the environmental refractive index. The refractive index sensor designed by the invention has the advantages of simple structure, easy processing and high sensing sensitivity, and can be used for realizing the sensing of the unmarked antigen-antibody binding rate.
Drawings
FIG. 1 is a schematic diagram of a three-dimensional periodic structure of a high-sensitivity U-shaped metal super-surface biosensor according to the present invention;
FIG. 2 is a schematic diagram of a metal micro-nano structure according to the present invention;
FIG. 3 is a graph of the sensitivity function of the sensor fitted to an ambient refractive index in the range of 1.3 to 1.5 in the example.
Description of the reference numerals
1-substrate, 2-metal micro-nano structure.
Detailed Description
The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-2, the high-sensitivity U-shaped metal super-surface biosensor comprises a substrate 1 and metal micro-nano structures 2 arranged on the substrate 1, wherein the metal micro-nano structures 2 are U-shaped, the U-shaped included angle of the metal micro-nano structures 2 is 90 degrees, and the metal micro-nano structures 2 are arranged in a periodic array along the X and Y directions.
The periods of the metal micro-nano structure 2 in the X direction and the Y direction are both 400nm.
The metal micro-nano structure 2 is noble metal gold.
The length a of the U-shaped unit structure of the metal micro-nano structure 2 is 200nm, the distance b between the bottoms of the U-shaped unit structures is 80nm, the distance c between two arms of the U-shaped unit structure is 100nm, and the height h of the U-shaped unit structure is 30nm.
The material of the substrate 1 is silicon dioxide, and the refractive index of the substrate 1 is 1.45.
The biosensor adopts a broad spectrum light source to vertically irradiate an emission signal to the surface of the biosensor, and a spectrometer is adopted to receive a reflection signal.
The range of the working of the biosensor in the near infrared wavelength is 1.5-2.0 μm.
The detection range of the biosensor to the outside environment refractive index is 1.3-1.5.
The embodiment is as follows: dropping a solution to be detected on the surface of the biosensor, vertically approaching an optical fiber to the surface, observing the change of a resonance position through a spectrometer, and obtaining a result as shown in figure 3, wherein when the refractive index of the solution to be detected is increased from 1.3 to 1.5, a red shift phenomenon appears at the resonance position along with the increase of the refractive index, the transmission value does not obviously change along with the change of the refractive index, when the environmental refractive index changes by 0.02, the resonance position moves by at least more than 15nm, the rule of the resonance position along with the change of the refractive index is further fitted, and through calculation, in a refractive index change interval, the linear relation between a resonance wavelength (lambda) and the refractive index (n) is expressed as: λ =728.1+843.1 + n, the refractive index sensitivity S of the designed refractive index sensor in this interval is 843.1nm/RIU.
The invention has simple structural design, easy processing, high sensitivity, no need of marking and simple operation, and the concentration and the sensing performance of the solution to be measured can be obtained by observing the change of the resonance position through the spectrometer.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (9)

1. The high-sensitivity U-shaped metal super-surface biosensor is characterized by comprising a substrate and metal micro-nano structures arranged on the substrate, wherein the metal micro-nano structures are U-shaped, the U-shaped included angle of the metal micro-nano structures is 90 degrees, and the metal micro-nano structures are arrayed periodically along the X direction and the Y direction.
2. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the metal micro-nano structure has a period of 400nm in both X and Y directions.
3. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the metal micro-nano structure is noble metal gold.
4. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the length of the U-shaped unit structure of the metal micro-nano structure is 200nm, the distance between the bottoms of the U-shaped unit structures is 80nm, the distance between the two arms of the U-shaped unit structure is 100nm, and the height of the U-shaped unit structure is 30nm.
5. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the substrate is made of silicon dioxide, and the refractive index of the substrate is 1.45.
6. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the biosensor uses a broad-spectrum light source to vertically incident emission signals on the surface of the biosensor, and uses a spectrometer to receive reflection signals.
7. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the biosensor works in the near infrared wavelength range of 1.5-2.0 μm.
8. The high-sensitivity U-shaped metal super-surface biosensor as claimed in claim 1, wherein the detection range of the biosensor to the external environment refractive index is 1.3-1.5.
9. The use method of the biosensor based on any one of claims 1 to 8 is characterized in that the metal micro-nano structure is positioned on a substrate, a light source is adopted as a plane wave, an electric field of the biosensor is polarized along an x axis and vertically incident along a-z axis, a magnetic resonance mode of the metal micro-nano structure is excited, and the position of a resonance peak displayed by a spectrometer represents the refractive index of a biological solution to be measured.
CN202211250320.3A 2022-10-13 2022-10-13 High-sensitivity U-shaped metal super-surface biosensor and use method thereof Pending CN115406869A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116953828A (en) * 2023-09-12 2023-10-27 之江实验室 Multiband absorber and design method thereof

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Publication number Priority date Publication date Assignee Title
CN106290270A (en) * 2016-07-22 2017-01-04 浙江大学 A kind of Fluorescence Increasing structure based on U-shaped metal array structure and system
CN114264627A (en) * 2021-12-20 2022-04-01 河南工业大学 Terahertz sensor and using method thereof
CN114858754A (en) * 2022-04-29 2022-08-05 聊城大学 Novel refractive index biosensor and manufacturing method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106290270A (en) * 2016-07-22 2017-01-04 浙江大学 A kind of Fluorescence Increasing structure based on U-shaped metal array structure and system
CN114264627A (en) * 2021-12-20 2022-04-01 河南工业大学 Terahertz sensor and using method thereof
CN114858754A (en) * 2022-04-29 2022-08-05 聊城大学 Novel refractive index biosensor and manufacturing method thereof

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116953828A (en) * 2023-09-12 2023-10-27 之江实验室 Multiband absorber and design method thereof
CN116953828B (en) * 2023-09-12 2024-03-12 之江实验室 Multiband absorber and design method thereof

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Application publication date: 20221129