CN115399189A - Lyophyllum decastes strain and liquid strain production and cultivation method - Google Patents
Lyophyllum decastes strain and liquid strain production and cultivation method Download PDFInfo
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 9
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a Lyophyllum decastes strain and a liquid strain production and cultivation method, wherein the Lyophyllum decastes strain is obtained by separating wild Lyophyllum decastes tissue, and the preservation number is as follows: CGMCC No.23846, the liquid strain preparation and cultivation method comprises the following steps: s1: preparing a liquid culture medium; s2: preparing liquid strains; s3: preparing culture materials; s4: inoculating and culturing; s5: fruiting management; s6: harvesting; the invention adopts liquid stock to replace solid stock, greatly shortens the period of hypha culture, ensures consistent fungus age of strains, regular fruiting, improves the biological conversion rate and correspondingly shortens the culture period.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a Lyophyllum decastes strain and a liquid strain production and cultivation method.
Background
Lyophyllum decastes belongs to the genus Lyophyllum of the family Lyophyllaceae and the subdivision Basidiomycotina. The other names are northern aeolian fungi, psychrophile, sheep and the like, and the commercial name is the velvet antler mushroom. It is mainly distributed in Yunnan, jiangsu, liaoning, jilin, heilongjiang and other areas of China, and has delicious taste and rich nutrition, and can be eaten both in fresh products and in dried products. The fruiting body is rich in beta-1, 3-D glucan and beta-1, 6-D glucan, has good inhibition effects on preventing and treating cancer cells, specific dermatitis and the like, has the effects of reducing blood fat, improving immunity, producing blood and the like, is a precious edible fungus for both food and medicine, is popular with people, and has wide future market prospect.
In recent years, the lyophyllum decastes has realized industrial production and has large market demand, but the properties of the production strains are difficult to maintain at present, and the problems of poor anti-microbial capacity, very low biological conversion rate, generally only 47.5 percent, very long cultivation period and the like exist, so the development of the lyophyllum decastes industry is seriously influenced, and therefore, the lyophyllum decastes production strains with high yield and excellent properties are urgently required to be cultivated; therefore, the method for producing and cultivating the lyophyllum decastes strain and the liquid strain is provided aiming at the problems.
Disclosure of Invention
In order to make up for the defects of the prior art and solve the problems of poor antibacterial capability, long cultivation period and low biological conversion rate of the lyophyllum decastes, the invention provides a lyophyllum decastes strain and a liquid strain production and cultivation method.
A Lyophyllum decastes strain is obtained by tissue isolation of wild Lyophyllum decastes, specifically ZJLZS001, and is classified and named as Lyophyllum decastes, latin name is Lyopyllum decastes; the name of the preservation unit is China general microbiological culture Collection center (CGMCC); the address is No. 3 of Xilu No. 1 of Beijing, chaoyang, north Chen; the preservation date is 2021, 11 months and 08 days, and the preservation number is CGMCC No.23846; the culture medium is rich PDA culture medium (potato 200g, glucose 20g, agar 15-18g, peptone 6g, yeast powder 3g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, and distilled water 1L.) and is cultured in dark environment at 25 deg.C.
A preparation and cultivation method of a lyophyllum decastes liquid strain comprises the following steps:
s1: preparing a liquid culture medium;
s2: preparing liquid strains;
s3: preparing cultivation compost;
s4: inoculating and culturing;
s5: fruiting management;
s6: and (6) harvesting.
Preferably, in S1, the preparation of the liquid culture medium comprises the following steps:
weighing 200g of potatoes, slicing, putting into boiling water, boiling for 20 minutes, and filtering 1000ml of filtrate by using 4-8 layers of gauze; adding glucose 20g, yeast powder 3g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g and VB into the filtrate 1 1, 1 piece of the composition; sterilizing at 121 deg.C and 0.15MPa for 25 min.
In S2, the preparation of the liquid strain comprises the following steps: inoculating the isolated Lyophyllum decastes test tube strain into the liquid culture medium, and culturing in a shaker at 25 deg.C and rotation speed of 160rpm/min for 7-10 days.
Preferably, in S3, the preparation of the cultivation medium includes the following steps:
uniformly mixing the cultivation compost, adjusting the water content to 60-65% and the pH value to 6.0-7.0 to obtain the cultivation compost; the culture compost obtained in the above step is filled into 1150ml culture bottles, and sterilized for 120min at 121 ℃ under 0.15 MPa.
Preferably, the cultivation compost comprises three different formulas, specifically:
formula a: 40 parts of straw, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime;
and (b) formula: 40 parts of corncobs, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime;
and (c) formula: 40 parts of cottonseed hulls, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime.
Preferably, in S4, the inoculating and culturing comprises: inoculating the liquid strain of Lyophyllum decastes to sterilized culture bottle compost, culturing at 20-25 deg.C in dark for 60-90 days, and transferring to fruiting room for fruiting after the culture bottle is full of mycelia.
Preferably, in S5, the fruiting management includes: transferring the cultivation bottle full of mycelia to a fruiting room, and culturing at 18-22 deg.C, humidity of 80-95% and CO 2 The fruiting management is carried out under the conditions of the concentration of 600-1500ppm and the illumination intensity of 200-500lx.
The invention has the advantages that:
1. provides a domesticated and preserved Lyophyllum decastes strain.
2. The invention adopts liquid stock to replace solid stock, greatly shortens the period of hypha culture, ensures consistent fungus age of strains, regular fruiting, improves the biological conversion rate and correspondingly shortens the culture period.
3. According to the invention, the cultivation medium is used as the only variable, three new cultivation formulas are screened through the first to third embodiments, the mushrooms can grow, the raw materials are easy to obtain, the mushroom bodies are large, the yield is high, and the biological efficiency is up to more than 70%; wherein the hyphae of the formula a grows fastest, buds are earliest and have good shape, the maximum mass of a single flower reaches 33.5g, the average yield is 176.47 g/bottle, and the biological efficiency is 70.59%; the yield of the formula b is highest, the average yield is 187.65 g/bottle, and the biological efficiency is 75.06%; the yield of the formula c is close to that of the formula a, and is 176.35 g/bottle, and the biological efficiency is 70.54 percent.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without inventive labor.
FIG. 1 is a flow chart of a method for cultivating a Lyophyllum decastes strain according to the present invention;
FIG. 2 is a drawing of a physical specimen of a wild Lyophyllum decastes according to the present invention;
FIG. 3 is a phylogenetic tree diagram of the molecular identification of strains according to the invention;
FIG. 4 is a drawing of a Lyophyllum decastes prepared by the culture medium of formula a in the invention;
FIG. 5 is a drawing of a Lyophyllum decastes prepared by the cultivation medium of formula b in the present invention;
FIG. 6 is a drawing of Lyophyllum decastes prepared by the cultivation medium of formula c in the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
please refer to FIGS. 1-6, a Lyophyllum decastes strain, which is obtained by tissue isolation of wild Lyophyllum decastes, specifically ZJLZS001, and is classified and named as Lyophyllum decastes, latin under the name Lyopyllum decastes; the name of the preservation unit is China general microbiological culture Collection center (CGMCC); the address is No. 3 of Xilu No. 1 of Beijing, chaoyang, north Chen; the preservation date is 2021, 11 months and 08 days, and the preservation number is CGMCC No.23846; the culture medium is rich PDA culture medium (potato 200g, glucose 20g, agar 15g, peptone 6g, yeast powder 3g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, and distilled water 1L.) and is cultured in dark environment at 25 deg.C.
A preparation and cultivation method of a lyophyllum decastes liquid strain comprises the following steps:
s1: preparing a liquid culture medium;
s2: preparing liquid strains;
s3: preparing cultivation compost;
s4: inoculating and culturing;
s5: fruiting management;
s6: and (6) harvesting.
Preferably, in S1, the preparation of the liquid medium comprises the following steps:
weighing 200g of potatoes, slicing, putting into boiling water, boiling for 20 minutes, and filtering 1000ml of filtrate by using 4 layers of gauze; adding glucose 20g, yeast powder 3g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g and VB into the filtrate 1 1, slicing; sterilizing at 121 deg.C under 0.15MPa for 25 min.
In S2, the preparation of the liquid strain comprises the following steps: inoculating the isolated Lyophyllum decastes test tube strain into the liquid culture medium, and culturing in a shaker at 25 deg.C and rotation speed of 160rpm/min for 7-10 days.
Preferably, in S3, the preparation of the cultivation medium includes the following steps:
uniformly mixing the cultivation compost, adjusting the water content to 60 percent and the pH value to 6.0 to obtain the cultivation compost; the culture compost obtained in the above step is filled into 1150ml culture bottles, and sterilized for 120min at 121 ℃ under 0.15 MPa.
Preferably, the cultivation compost specifically comprises:
40 parts of straw, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime;
preferably, in S4, the inoculating and culturing comprises: inoculating the liquid strain of Lyophyllum decastes to sterilized culture bottle compost, culturing at 20 deg.C in dark for 60 days, and transferring to fruiting room for fruiting management after mycelia overgrow the culture bottle.
Preferably, in S5, the fruiting management includes: transferring the cultivation bottle full of mycelia to a fruiting room, at 18 deg.C, humidity 80%, and CO 2 Concentration of 600ppm and illumination intensity of 200lxAnd (5) mushroom management.
The second embodiment:
pleurotus Nebrodensis strain shown in FIGS. 1-6 is a Lyophyllum decastes strain obtained by separating wild Lyophyllum decastes tissue, specifically ZJLZS001, named as Lyophyllum decastes by classification, latin brand as Lyopylum decastes; the name of the preservation unit is China general microbiological culture Collection center (CGMCC); the address is No. 3 of Xilu No. 1 Beijing, chaoyang, beicheng area; the preservation date is 2021, 11 months and 08 days, and the preservation number is CGMCC No.23846; the culture medium is rich PDA culture medium (potato 200g, glucose 20g, agar 15g, peptone 6g, yeast powder 3g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, and distilled water 1L.) and is cultured in dark environment at 25 deg.C.
A preparation and cultivation method of a lyophyllum decastes liquid strain comprises the following steps:
s1: preparing a liquid culture medium;
s2: preparing liquid strains;
s3: preparing cultivation compost;
s4: inoculating and culturing;
s5: fruiting management;
s6: and (6) harvesting.
Preferably, in S1, the preparation of the liquid medium comprises the following steps:
weighing 200g of potatoes, slicing, putting into boiling water, boiling for 20 minutes, and filtering 1000ml of filtrate by using 4 layers of gauze; adding glucose 20g, yeast powder 3g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g and VB into the filtrate 1 1, 1 piece of the composition; sterilizing at 121 deg.C under 0.15MPa for 25 min.
In S2, the preparation of the liquid strain comprises the following steps: inoculating the isolated Lyophyllum decastes test tube strain into the liquid culture medium, and culturing in a shaker at 25 deg.C and rotation speed of 160rpm/min for 7-10 days.
Preferably, in S3, the preparation of the cultivation medium includes the following steps:
uniformly mixing the cultivation compost, adjusting the water content to 60% and the pH value to 6.0 to obtain the cultivation compost; the cultivation compost obtained in the above step is filled into 1150ml cultivation bottles, and sterilized for 120min at 121 ℃ under 0.15 MPa.
Preferably, the cultivation compost specifically comprises:
40 parts of corncobs, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime.
Preferably, in S4, the inoculating and culturing comprises: inoculating the liquid strain of Lyophyllum decastes to sterilized culture bottle compost, culturing at 20 deg.C in dark for 60 days, and transferring to fruiting room for fruiting management after mycelia overgrow the culture bottle.
Preferably, in S5, the fruiting management includes: transferring the cultivation bottle full of mycelia to a fruiting room, at 18 deg.C, humidity 80%, and CO 2 And (4) performing fruiting management under the conditions of concentration of 600ppm and illumination intensity of 200 lx.
Example three:
please refer to FIGS. 1-6, a Lyophyllum decastes strain, which is obtained by tissue isolation of wild Lyophyllum decastes, specifically ZJLZS001, and is classified and named as Lyophyllum decastes, latin under the name Lyopyllum decastes; the name of the preservation unit is China general microbiological culture Collection center (CGMCC); the address is No. 3 of Xilu No. 1 of Beijing, chaoyang, north Chen; the preservation date is 2021, 11 months and 08 days, and the preservation number is CGMCC No.23846; the culture medium is rich PDA culture medium (potato 200g, glucose 20g, agar 15g, peptone 6g, yeast powder 3g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, and distilled water 1L.) and is cultured in dark environment at 25 deg.C.
A preparation and cultivation method of a lyophyllum decastes liquid strain comprises the following steps:
s1: preparing a liquid culture medium;
s2: preparing liquid strains;
s3: preparing cultivation compost;
s4: inoculating and culturing;
s5: fruiting management;
s6: and (6) harvesting.
Preferably, in S1, the preparation of the liquid medium comprises the following steps:
weighing 200g of potatoes, slicing, putting into boiling water, boiling for 20 minutes, and filtering 1000ml of filtrate by using 4 layers of gauze; adding glucose 20g, yeast powder 3g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g and VB into the filtrate 1 1, slicing; sterilizing at 121 deg.C under 0.15MPa for 25 min.
In S2, the preparation of the liquid strain comprises the following steps: inoculating the isolated Lyophyllum decastes test tube strain into the liquid culture medium, and culturing in a shaker at 25 deg.C and rotation speed of 160rpm/min for 7-10 days.
Preferably, in S3, the preparation of the cultivation medium includes the following steps:
uniformly mixing the cultivation compost, adjusting the water content to 60 percent and the pH value to 6.0 to obtain the cultivation compost; the culture compost obtained in the above step is filled into 1150ml culture bottles, and sterilized for 120min at 121 ℃ under 0.15 MPa.
Preferably, the cultivation compost specifically comprises:
40 parts of cottonseed hulls, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime.
Preferably, in S4, the inoculating and culturing comprises: inoculating the liquid strain of Lyophyllum decastes to sterilized culture bottle compost, culturing at 20 deg.C in dark for 60 days, and transferring to fruiting room for fruiting management after mycelia overgrow the culture bottle.
Preferably, in S5, the fruiting management includes: transferring the cultivation bottle full of mycelia to a fruiting room, at 18 deg.C, humidity 80%, and CO 2 And (4) carrying out fruiting management under the conditions of the concentration of 600ppm and the illumination intensity of 200 lx.
Wherein the harvesting operation is picking timely before the pileus is flat and the color of the edge becomes light, as shown in fig. 4, fig. 5 and fig. 6.
Specifically, the hypha growth rate, the bottle filling time and the bud forming time of the three culture formulas of the lyophyllum decastes are shown in the following table under the culture composts of the three different formulas:
hypha growth rate, bottle filling time and budding time table of three cultivation formulas of lyophyllum decastes
| Hypha growth rate cm/d | Time of bottle full of hyphae/d | Bud time/d | |
| a | 0.24 | 62 | 67 |
| b | 0.17 | 89 | 94 |
| c | 0.16 | 91 | 96 |
Under the cultivation culture materials of three different formulas, the agronomic character conditions of the fruit bodies of the three cultivation formulas of the Lyophyllum decastes are shown in the following table:
agronomic character condition table of fruiting bodies of three cultivation formulas of Lyophyllum decastes
| Pileus diameter/mm | Diameter of stipe/mm | Length/cm of stipe | Heaviest single/g | Average fresh weight per bottle/g | Biological efficiency/%) | |
| a | 43.51 | 22.54 | 8.76 | 33.5 | 176.47 | 70.59 |
| b | 54.24 | 17.77 | 8.24 | 25.7 | 187.65 | 75.06 |
| c | 59.09 | 19.42 | 7.95 | 28.3 | 176.35 | 70.54 |
The Lyophyllum decastes strain is separated from a wild Lyophyllum decastes fruiting body specimen, the number of the Lyophyllum decastes strain is ZJLZS001, the strain is sent to China general microbiological culture Collection center (CGMCC) to be preserved at 11/8/2021, and the preservation number of the patent strain is as follows: CGMCC No.23846; the specimens were collected in the Wulong mountain landscape, dandong, liaoning province, 6/5/2020 as shown in FIG. 2;
molecular identification of the strain:
DNA extraction and ribosome internal transfer spacer (ITS sequence) sequencing are carried out on mycelium of a Lyophyllum decastes strain ZJLZS001 obtained by separation, used primers are primer pairs consisting of ITS4 and ITS5, then the sequence of a tested sample is submitted to an NCBI database for BLAST sequence comparison analysis, and the result shows that the homology with Lyophyllum decastes is 99.21%;
downloading sequences of different species of the same genus from a GenBank database, using a MEGA software component NJ phylogenetic tree and default parameters, and performing Bootstrap test 1000 times; phylogenetic tree shows: the strain ZJLZS001 and 4 Lyophyllum decastes (Lyophyllum decastes) strains are gathered in the same branch, and the results of BLAST comparison and phylogenetic tree are combined, so that the sequenced strain ZJLZS001 is Lyophyllum decastes as shown in figure 3.
The invention adopts liquid to replace solid stock culture, greatly shortens the period of mycelium culture, ensures that the liquid strains have consistent fungus age and uniform fruiting, improves the anti-bacteria capability of the strains to a certain extent, and ensures that the biological conversion rate of the lyophyllum decastes cultivated according to the invention is improved and the cultivation period is correspondingly shortened.
According to the invention, the cultivation medium is used as the only variable, and through the first embodiment to the third embodiment, three new cultivation formulas are screened, so that the mushrooms can grow, the raw materials are easy to obtain, the mushroom bodies are large, the yield is high, and the biological efficiency is up to more than 70%; wherein the hypha of the formula a grows fastest, the bud is earliest, the shape of each bud is good, the maximum mass of a single flower reaches 33.5g, the average yield is 176.47 g/bottle, and the biological efficiency is 70.59%; the yield of the formula b is highest, the average yield is 187.65 g/bottle, and the biological efficiency is 75.06%; the yield of the formula c is close to that of the formula a, and is 176.35 g/bottle, and the biological efficiency is 70.54 percent.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.
Claims (7)
1. A Lyophyllum decastes strain is characterized in that: the Lyophyllum decastes strain is obtained by separating the tissues of wild Lyophyllum decastes, specifically ZJLZS001, and is preserved in China general microbiological culture Collection center (CGMCC), with the preservation number: CGMCC No.23846.
2. A method for preparing and cultivating lyophyllum decastes liquid strains is characterized in that: the method comprises the following steps:
s1: preparing a liquid culture medium;
s2: preparing liquid strains;
s3: preparing cultivation compost;
s4: inoculating and culturing;
s5: fruiting management;
s6: and (6) harvesting.
3. The method for producing and cultivating lyophyllum decastes liquid spawn according to claim 2, which is characterized in that: in S1, the preparation of the liquid culture medium comprises the following steps:
weighing 200g of potatoes, slicing, putting into boiling water, boiling for 20 minutes, and filtering 1000ml of filtrate by using 4-8 layers of gauze; adding glucose 20g, yeast powder 3g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g and VB into the filtrate 1 1, 1 piece of the composition; sterilizing at 121 deg.C under 0.15MPa for 25 min.
4. The method for producing and cultivating lyophyllum decastes liquid spawn according to claim 2, which is characterized in that: in S3, the preparation of the cultivation compost comprises the following steps:
uniformly mixing the cultivation compost, adjusting the water content to 60-65% and the pH value to 6.0-7.0 to obtain the cultivation compost; the cultivation compost obtained in the above step is filled into 1150ml cultivation bottles, and sterilized for 120min at 121 ℃ under 0.15 MPa.
5. The method for producing and cultivating lyophyllum decastes liquid spawn according to claim 4, which is characterized in that: the preparation of the cultivation compost comprises three different formulas, specifically:
formula a: 40 parts of straw, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime;
and (b) a formula: 40 parts of corncobs, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime;
and (c) formula: 40 parts of cottonseed hulls, 38 parts of sawdust, 12 parts of bran, 8 parts of corn flour and 2 parts of lime.
6. The method for producing and cultivating lyophyllum decastes liquid spawn according to claim 2, which is characterized in that: in the S4, the inoculation and culture conditions are that the temperature is 20-25 ℃, and the culture is carried out for about 60 days under the dark condition.
7. The method for producing and cultivating lyophyllum decastes liquid spawn according to claim 2, wherein the method comprises the following steps: in S5, the fruiting management conditions comprise temperature of 18-22 deg.C, humidity of 80-95%, and CO 2 The concentration is 600-1500ppm, and the illumination intensity is 200-500lx.
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