CN115381892B - Preparation method of blackberry lily extract - Google Patents
Preparation method of blackberry lily extract Download PDFInfo
- Publication number
- CN115381892B CN115381892B CN202211242258.3A CN202211242258A CN115381892B CN 115381892 B CN115381892 B CN 115381892B CN 202211242258 A CN202211242258 A CN 202211242258A CN 115381892 B CN115381892 B CN 115381892B
- Authority
- CN
- China
- Prior art keywords
- ethanol
- blackberry lily
- extract
- weighing
- porous resin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000596154 Belamcanda Species 0.000 title claims abstract description 88
- 239000000284 extract Substances 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 153
- 239000011347 resin Substances 0.000 claims abstract description 42
- 229920005989 resin Polymers 0.000 claims abstract description 42
- 238000005303 weighing Methods 0.000 claims abstract description 32
- 239000003480 eluent Substances 0.000 claims abstract description 26
- 238000002791 soaking Methods 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 16
- 239000002904 solvent Substances 0.000 claims abstract description 11
- 238000011068 loading method Methods 0.000 claims abstract description 8
- 239000000126 substance Substances 0.000 claims description 31
- 238000003756 stirring Methods 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 11
- 230000006837 decompression Effects 0.000 claims description 9
- 230000001376 precipitating effect Effects 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 238000010981 drying operation Methods 0.000 claims description 3
- 238000001125 extrusion Methods 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 11
- 206010011224 Cough Diseases 0.000 abstract description 5
- 206010062717 Increased upper airway secretion Diseases 0.000 abstract description 5
- 208000006673 asthma Diseases 0.000 abstract description 5
- 238000000605 extraction Methods 0.000 abstract description 5
- 208000026435 phlegm Diseases 0.000 abstract description 5
- 239000003053 toxin Substances 0.000 abstract description 3
- 231100000765 toxin Toxicity 0.000 abstract description 3
- 210000000440 neutrophil Anatomy 0.000 description 8
- 210000003979 eosinophil Anatomy 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 206010068319 Oropharyngeal pain Diseases 0.000 description 3
- 201000007100 Pharyngitis Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 241000700159 Rattus Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 241001480043 Arthrodermataceae Species 0.000 description 1
- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000001974 Hyaluronidases Human genes 0.000 description 1
- 241001113425 Iridaceae Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 241000282376 Panthera tigris Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000004856 capillary permeability Effects 0.000 description 1
- 230000037304 dermatophytes Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000001882 diuretic effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000588 effect on asthma Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/10—Expectorants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/14—Antitussive agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses a preparation method of blackberry lily extract, which comprises the following steps: s1, weighing the dried blackberry lily and placing the dried blackberry lily on an operation table for preparation operation; s2, soaking the weighed blackberry lily in ethanol with a certain proportion, extracting for about 3 times, and filtering and recovering the soaked ethanol to obtain a pre-extract; s3, D is to 101 Standing the porous resin on an operation table, and loading the pre-extraction solution on the D 101 A fully porous resin column; s4, eluting with water with the volume of (1-8) times of v/w resin, eluting with 80% -100% ethanol with the volume of (8-15) times of v/w resin to obtain ethanol eluent, and collecting with a solvent barrel; s5, filtering the ethanol eluent. The preparation method of the blackberry lily extract provided by the invention has the advantages that the preparation process is simple and convenient, the blackberry lily extract has an important curative effect on heat toxin phlegm fire stagnation and cough and asthma, the practicability of the blackberry lily extract preparation is greatly improved, and the actual application and operation are facilitated.
Description
Technical Field
The invention belongs to the technical field of preparation of blackberry lily extracts, and particularly relates to a preparation method of blackberry lily extracts.
Background
The existing blackberry lily extract is a dry rhizome extract of blackberry lily of Iridaceae, mainly contains flavonoid and other components, is also called a tiger fan and a ghost fan, has bitter and cold nature, has the effects of clearing heat and detoxicating, resolving phlegm and relieving sore throat, and is commonly used for heat toxin and phlegm fire stagnation, sore throat, excessive phlegm and saliva accumulation, cough and asthma.
In actual life, the pharmacological action of the blackberry lily extract is relatively wide, and the action is mainly summarized as six points: 1. the anti-inflammatory effect can inhibit the increase of capillary permeability of rat skin caused by acetic acid, can inhibit the effects of hyaluronidase edema and the like of rats, can reduce blood pressure continuously by using blackberry lily alcohol extract, has obvious effect, has an inhibiting effect on common pathogenic dermatophytes, has a fourth and antiviral effect, particularly has an inhibiting effect on viruses in throat diseases and other viruses, has a fifth and a third effect on enhancing salivary secretion, can quickly improve the permeability of capillary vessels, and has a sixth and a diuretic effect.
Therefore, the blackberry lily extract plays an important therapeutic role in actual life, plays a better therapeutic effect on cough, asthma and sore throat, and is particularly important, but in the prior art, the blackberry lily extract is formed by a method, but in the existing blackberry lily extract preparation, the operation is more complicated, more errors are easy to occur, the practicability of the blackberry lily extract preparation method is greatly reduced, and the actual application and operation are not facilitated.
The invention comprises the following steps:
the present invention aims to solve the above problems and provide a method for preparing blackberry lily extract, which solves the problems mentioned in the background art.
In order to solve the problems, the invention provides a technical scheme that:
a preparation method of blackberry lily extract comprises the following steps:
s1, weighing the dried blackberry lily and placing the dried blackberry lily on an operation table for preparation operation;
s2, soaking the weighed blackberry lily in ethanol with a certain proportion, extracting for about 3 times, and filtering and recovering the soaked ethanol to obtain a pre-extract;
s3, D is to 101 Standing the porous resin on an operation table, and loading the pre-extraction solution on the D 101 A fully porous resin column;
s4, eluting with water with the volume of (1-8) times of v/w resin, eluting with 80% -100% ethanol with the volume of (8-15) times of v/w resin to obtain ethanol eluent, and collecting with a solvent barrel;
s5, filtering the ethanol eluent, controlling the temperature and precipitating;
s6, concentrating the precipitated substance under reduced pressure to obtain a paste, and drying the paste to obtain the blackberry lily extract.
Preferably, the specific manufacturing steps in the step S1 placed on the operation table to prepare are as follows:
s11, before preparation, the operation table is required to be disinfected;
s12, carrying out detailed inspection on the weighing instrument, weighing the weighing instrument to check whether the instrument is normal or not, and ensuring the accuracy of the prepared data;
s13, standing the dried blackberry lily on a weighing instrument for weighing operation, weighing 100g, and recording.
Preferably, the specific operation steps of filtering and recovering the pre-extracted solution in the step S2 are as follows:
s21, weighing 100g of dry blackberry lily, placing the dry blackberry lily in a container, and weighing 230g of ethanol by a weighing instrument;
s22, diluting with a proper amount of water and 550g of ethanol, wherein the diluted ethanol is an 80% ethanol solution;
s23, mixing 550g of 80% ethanol with 100g of dry blackberry lily, soaking, and stirring while soaking, wherein the stirring is as follows; the method comprises the steps of using a stirring rod to stir clockwise for about 5 minutes every 15 minutes, wherein the stirring time is 1 hour, observing ethanol and blackberry lily in detail, recording the color of blackberry lily, soaking the blackberry lily for 1 hour, standing the blackberry lily for 20 minutes, extracting the blackberry lily, standing the blackberry lily after the extraction is finished, repeating the operation for 3 times, recovering the soaked ethanol through a filtering device, placing the recovered ethanol in a spare solvent barrel, and using the ethanol as a pre-extracting solution.
Preferably, the extract in the step S3 is applied to D 101 The specific operation steps of the full porous resin column are as follows:
s31, using disinfectant to treat D 101 Sterilizing the porous resin, and placing it inStanding a clean operation table;
s32, loading the pre-extract into the D 101 Full porous resin column D 101 The dosage of the fully porous resin is 300g;
s33, performing decompression drying operation on the mixture, wherein the decompression drying temperature is 70 ℃.
Preferably, the ethanol eluent is obtained in step S4, and the specific operation steps of collecting using a solvent barrel are as follows:
S41、D 101 the full porous resin needs to be eluted with water with the volume of (1-8) times of v/w resin, then the water is drained and added with 80% -100% ethanol with the volume of (8-15) times of v/w resin for eluting, the ethanol is drained to obtain ethanol eluent, and the ethanol eluent which is collected to 100ml is used for subsequent operation.
Preferably, the ethanol eluent in step S5 is filtered, concentrated and then precipitated as follows:
s51, slowly pouring the ethanol eluent into the filter container, and precipitating for 24 hours;
s52, placing the precipitated substance into a device with the temperature of minus 10 ℃ for standing, wherein the precipitated substance can be coagulated under the condition of lower temperature, so that the coagulated substance is more stable and is not easy to volatilize.
Preferably, in the step S6, the precipitated substance is concentrated under reduced pressure to obtain a paste, and the paste is dried as follows:
s61, putting the precipitated substances into a closed tank, introducing oxygen into the closed tank, mixing the precipitated substances, and performing decompression operation inside the substances;
s62, extruding and concentrating the decompressed substance to obtain paste with extrusion strength of 500 mpa;
and S63, drying the paste in a collection bottle at the temperature of 30 ℃ for 6 hours to obtain the blackberry lily extract.
Preferably, the time of each ethanol soaking in the step S2 is 100-120 minutes.
Preferably, the weight of the ethanol in the step S2 is 5-10 times of that of the blackberry lily.
The beneficial effects of the invention are as follows: according to the preparation method of the blackberry lily extract, the preparation process is simple and convenient, the blackberry lily extract has an important curative effect on heat toxin and phlegm fire stagnation and cough and asthma, the blackberry lily can be fully reacted with ethanol through the mixing and stirring effects of drying blackberry lily, the accuracy of subsequent experiments can be ensured, the precipitated substances can be rapidly aggregated through the standing effect of placing the precipitated substances into a device with the temperature of-10 ℃, the substances can be more stable and are not easy to volatilize, the practicability of the preparation of the blackberry lily extract is greatly improved, and the preparation method is beneficial to practical application and operation.
Description of the drawings:
for ease of illustration, the invention is described in detail by the following detailed description and the accompanying drawings.
FIG. 1 is a schematic flow chart of a preparation method of blackberry lily extract.
The specific embodiment is as follows:
as shown in fig. 1, the following technical scheme is adopted in this embodiment.
Examples:
a preparation method of blackberry lily extract comprises the following steps:
s1, weighing the dried blackberry lily, and placing the dried blackberry lily on an operation table for preparation operation so as to ensure the authenticity of the subsequent comparison data;
s2, soaking the weighed blackberry lily in ethanol with a certain proportion, extracting for about 3 times, and filtering and recovering the soaked ethanol to obtain a pre-extract for facilitating the accuracy of data;
s3, D is to 101 Standing the porous resin on an operation table, and loading the pre-extraction solution on the D 101 The full porous resin column is convenient for obtaining experimental data;
s4, eluting with water with the volume of 8 times of v/w resin, eluting with 80% ethanol with the volume of 8 times of v/w resin to obtain ethanol eluent, and collecting with a solvent barrel;
s5, filtering the ethanol eluent, controlling the temperature and precipitating;
s6, concentrating the precipitated substance under reduced pressure to obtain a paste, and drying the paste to obtain the blackberry lily extract.
The specific manufacturing steps of the step S1 placed on the operation table to prepare are as follows:
s11, before preparation, the operation table is required to be disinfected;
s12, carrying out detailed inspection on the weighing instrument, weighing the weighing instrument to check whether the instrument is normal or not, and ensuring the accuracy of the prepared data;
s13, standing the dried blackberry lily on a weighing instrument for weighing operation, weighing 100g, and recording.
The specific operation steps of filtering and recovering the pre-extracted liquid in the step S2 are as follows:
s21, weighing 100g of dry blackberry lily, placing the dry blackberry lily in a container, and weighing 230g of ethanol by a weighing instrument;
s22, diluting with a proper amount of water and 550g of ethanol, wherein the diluted ethanol is an 80% ethanol solution;
s23, mixing 550g of 80% ethanol with 100g of dry blackberry lily, soaking, and stirring while soaking, wherein the stirring is as follows; the method comprises the steps of using a stirring rod to stir clockwise for about 5 minutes every 15 minutes, wherein the stirring time is 1 hour, observing ethanol and blackberry lily in detail, recording the color of blackberry lily, soaking the blackberry lily for 1 hour, standing the blackberry lily for 20 minutes, extracting the blackberry lily, standing the blackberry lily after the extraction is finished, repeating the operation for 3 times, recovering the soaked ethanol through a filtering device, placing the recovered ethanol in a spare solvent barrel, and using the ethanol as a pre-extracting solution.
Wherein, the pre-extracting solution in the step S3 is applied to the D 101 The specific operation steps of the full porous resin column are as follows:
s31, using disinfectant to treat D 101 Sterilizing the porous resin, and placing the porous resin on a clean operation table for standingPlacing;
s32, loading the pre-extract into the D 101 Full porous resin column D 101 The dosage of the fully porous resin is 300g;
s33, performing decompression drying operation on the mixture, wherein the decompression drying temperature is 70 ℃.
Wherein, the ethanol eluent is obtained in the step S4, and the specific operation steps of collecting by using a solvent barrel are as follows:
S41、D 101 the fully porous resin needs to be eluted with water with the volume of 8 times of v/w resin, then the water is drained and 80% ethanol with the volume of 8 times of v/w resin is added for eluting, the ethanol is drained to obtain ethanol eluent, and the ethanol eluent is collected to 100ml for subsequent operation.
The specific steps of filtering the ethanol eluent in the step S5, concentrating and then precipitating are as follows:
s51, slowly pouring the ethanol eluent into the filter container, and precipitating for 24 hours;
s52, placing the precipitated substance into a device with the temperature of minus 10 ℃ for standing, wherein the precipitated substance can be coagulated under the condition of lower temperature, so that the coagulated substance is more stable and is not easy to volatilize.
In the step S6, the precipitated substance is concentrated under reduced pressure to obtain a paste, and the paste is dried as follows:
s61, putting the precipitated substances into a closed tank, introducing oxygen into the closed tank, mixing the precipitated substances, and performing decompression operation inside the substances;
s62, extruding and concentrating the decompressed substance to obtain paste with extrusion strength of 500 mpa;
and S63, drying the paste in a collection bottle at the temperature of 30 ℃ for 6 hours to obtain the blackberry lily extract.
Wherein, the time of each ethanol soaking in the step S2 is 120 minutes.
Wherein the weight of the ethanol in the step S2 is 5 times of that of the blackberry lily.
Comparative example 1 preparation of blackberry lily extract step:
firstly, weighing the dried blackberry lily, and placing the dried blackberry lily on an operation table for preparation operation;
secondly, soaking the weighed blackberry lily in ethanol with a certain proportion, extracting for 2 times, and filtering and recovering the soaked ethanol to obtain a pre-extract;
third step, D 101 Standing the porous resin on an operation table, and loading the extract on D 101 A fully porous resin column;
fourthly, eluting with water with the amount of 16 times of v/w resin to obtain eluent, and collecting the eluent by using a solvent barrel;
fifthly, filtering the eluent, controlling the temperature and precipitating;
and sixthly, concentrating the precipitated substance under reduced pressure to obtain a paste, and drying the paste to obtain the blackberry lily extract.
The invention adopts the example 1 and the comparative example 1 to respectively carry out corresponding comparative treatment, 40 patients suffering from cough and asthma are divided into 4 groups of 10 persons, wherein 1 group of 5 men and 5 women are implemented, the ages of the 5 men and the 5 women are all within the interval of 20-24, and the average age is about 22 years; in contrast, 5 men and women in group 1 had ages of 20-24, average ages of about 22 years, 6 men and women in group 2 had ages of 4, about 20-24, average ages of about 22 years, and 4 men and women in group 2 had ages of 6, about 20-24, average ages of about 22 years.
The experimental method comprises the following steps: patients were subjected to corresponding comparative treatments, 50ml of the blackberry lily extract prepared in example 1 was drunk by each person of example 1 with 50ml of warm water per day at 20 ℃, 50ml of the blackberry lily extract prepared in comparative example 1 was drunk by each person of example 1 with 50ml of warm water per day at 20 ℃, 50ml of the blackberry lily extract prepared in example 1 was drunk by each person of example 2 with 50ml of warm water per day at 20 ℃, 50ml of the blackberry lily extract prepared in comparative example 1 was drunk by each person of example 2 with 50ml of warm water per day at 20 ℃.
The judging method comprises the following steps: the counts of neutrophils and eosinophils at the very beginning and days 5, 10, 15, 20, 30 of most patients in the four groups were recorded and compared, with the results shown in the following table.
Neutrophils:
eosinophils:
the normal value of neutrophils is 2000-7000ml, while eosinophils belong to one of white blood cells, the proportion of neutrophils is 4% -8% in normal, and it is known from the table that after the patients in the 1 group and the 2 group eat medicines, the content of neutrophils and eosinophils approaches to the normal value after fifteenth day, and the content of neutrophils and eosinophils drops slowly over time before fifteen days, which means that the medicine effect of the medicine extracted by the invention is better, and the content of neutrophils and eosinophils in the patients in the 1 group and the 2 group is reduced in thirty days, but the dropping speed is slower, until the thirty days, the content of part of neutrophils and eosinophils in the patients does not enter the normal range, and the part of the patients do not experience improvement, so the curative effect of the blackberry lily extract prepared by the patients in the 1 group and the 2 group is greater than the curative effect of blackberry lily extract prepared by the 1 group and the 2 group.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The preparation method of the blackberry lily extract is characterized by comprising the following steps:
s1, weighing the dried blackberry lily and placing the dried blackberry lily on an operation table for preparation operation;
s2, soaking the weighed blackberry lily in ethanol with a certain proportion, extracting for 3 times, and filtering and recovering the soaked ethanol to obtain a pre-extract;
s3, D is to 101 Standing the porous resin on an operation table, and loading the extract on D 101 A fully porous resin column;
s4, eluting with water with the volume of (1-8) times of the volume of the v/w resin, eluting with 80% -100% ethanol with the volume of (8-15) times of the volume of the v/w resin to obtain ethanol eluent, and collecting with a solvent barrel;
s5, filtering the ethanol eluent, controlling the temperature and precipitating;
s6, concentrating the precipitated substance under reduced pressure to obtain a paste, and drying the paste to obtain a blackberry lily extract;
the specific steps of the step S5 are as follows:
s51, slowly pouring the ethanol eluent into the filter container, and precipitating for 24 hours;
s52, placing the precipitated substance into a device with the temperature of minus 10 ℃ for standing, wherein the precipitated substance can be coagulated under the condition of lower temperature, so that the coagulated substance is more stable and is not easy to volatilize.
2. The method for preparing blackberry lily extract according to claim 1, wherein the specific steps of preparing the blackberry lily extract in the step S1 are as follows:
s11, before preparation, the operation table is required to be disinfected;
s12, carrying out detailed inspection on the weighing instrument, weighing the weighing instrument to check whether the instrument is normal or not, and ensuring the accuracy of the prepared data;
s13, standing the dried blackberry lily on a weighing instrument for weighing operation, weighing 100g, and recording.
3. The method for preparing blackberry lily extract according to claim 1, wherein the specific operation steps of filtering and recovering the pre-extract in the step S2 are as follows:
s21, weighing 100g of dry blackberry lily, placing the dry blackberry lily in a container, and weighing 230g of ethanol by a weighing instrument;
s22, diluting with a proper amount of water and 550g of ethanol, wherein the diluted ethanol is an 80% ethanol solution;
s23, mixing 550g of 80% ethanol with 100g of dry blackberry lily, soaking, and stirring while soaking, wherein the stirring is as follows; stirring clockwise for 5 min with stirring rod every 15 min for 1 hr, observing ethanol and rhizoma Belamcandae in detail, recording the color of rhizoma Belamcandae, soaking for 1 hr, standing for 20 min, extracting, standing for 3 times, recovering ethanol after soaking, and placing the recovered ethanol in the empty solvent barrel for use as pre-extractive solution.
4. The method of claim 3, wherein the extract in step S3 is applied to the treated D 101 The specific operation steps of the full porous resin column are as follows:
s31, sterilizing the full-porous resin by using a sterilizing liquid, and placing the full-porous resin on a clean operation table for standing;
s32, loading the pre-extract into the D 101 Full porous resin column D 101 The dosage of the fully porous resin is 300g;
s33, performing decompression drying operation on the mixture, wherein the decompression drying temperature is 70 ℃.
5. The method for preparing blackberry lily extract according to claim 1, wherein the ethanol eluent is obtained in step S4, and the specific operation steps of collecting the ethanol eluent by using a solvent barrel are as follows:
S41、D 101 the full porous resin needs to be eluted with (1-8) times of v/w resin amount of water, then the water is drained and added with (8-15) times of v/w resin amount of 80% -100% ethanol for eluting, the ethanol is drained to obtain ethanol eluent, and the ethanol eluent is collected to 100ml for subsequent operation.
6. The method for preparing blackberry lily extract according to claim 1, wherein the step S6 is characterized in that the precipitated substance is concentrated under reduced pressure to obtain a paste, and the paste is dried as follows:
s61, putting the precipitated substances into a closed tank, introducing oxygen into the closed tank, mixing the precipitated substances, and performing decompression operation inside the substances;
s62, extruding and concentrating the decompressed substance to obtain paste with extrusion strength of 500 mpa;
and S63, drying the paste in a collection bottle at the temperature of 30 ℃ for 6 hours to obtain the blackberry lily extract.
7. The method according to claim 2, wherein the time of each ethanol soaking in step S2 is 100-120 minutes.
8. The method for preparing blackberry lily extract according to claim 2, wherein the weight of ethanol in the step S2 is 5-10 times of that of blackberry lily.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211242258.3A CN115381892B (en) | 2022-10-11 | 2022-10-11 | Preparation method of blackberry lily extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211242258.3A CN115381892B (en) | 2022-10-11 | 2022-10-11 | Preparation method of blackberry lily extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115381892A CN115381892A (en) | 2022-11-25 |
| CN115381892B true CN115381892B (en) | 2024-04-09 |
Family
ID=84127586
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211242258.3A Active CN115381892B (en) | 2022-10-11 | 2022-10-11 | Preparation method of blackberry lily extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115381892B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106963853A (en) * | 2017-04-11 | 2017-07-21 | 四川省中医药科学院 | A kind of Rhizoma Belamcandae total-flavonoid aglycone extract and its production and use |
-
2022
- 2022-10-11 CN CN202211242258.3A patent/CN115381892B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106963853A (en) * | 2017-04-11 | 2017-07-21 | 四川省中医药科学院 | A kind of Rhizoma Belamcandae total-flavonoid aglycone extract and its production and use |
Non-Patent Citations (1)
| Title |
|---|
| "射干有效组分大孔吸附树脂分离特性的研究";尤献民等;《辽宁中医杂志》;第40卷(第11期);第2328-2330页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115381892A (en) | 2022-11-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109260340B (en) | Traditional Chinese medicine composition and preparation method and application thereof | |
| CN111454378B (en) | Method for improving extraction amount of dendrobium officinale polysaccharide by utilizing low-temperature stress effect | |
| CN102860445A (en) | Preparation method for honeycomb element cream | |
| CN107513052A (en) | A kind of method that anthocyanidin is extracted in the fruit pomace from Black Box Tracing | |
| CN106810904A (en) | A kind of extracting method of anthocyan composition | |
| CN110075213A (en) | The method of extraction purification general flavone and total saposins from Rootlet Ophiopogonis | |
| CN106279462B (en) | The preparation method of the extracting method of astragalus polyose and astragalus polysaccharides oral liquid for animals | |
| CN115381892B (en) | Preparation method of blackberry lily extract | |
| CN110283257A (en) | A kind of extracting method of Radix Angelicae Sinensis polysaccharide | |
| CN114404444B (en) | Polysaccharide composition for promoting expression of aquaporin, and preparation method and application thereof | |
| CN114107418B (en) | Preparation method of ginseng polypeptide | |
| CN1806831B (en) | Medicine for treating psoriasis and its preparing process | |
| CN112569287B (en) | Anti-allergy itching-relieving plant composition and preparation method and application thereof | |
| CN114041516A (en) | Method for increasing GABA content in mulberry leaf tea | |
| CN113416117B (en) | Method for extracting nerolidol from schisandra chinensis | |
| CN109369398A (en) | A kind of maize alcohol stillage Content of Chlorogenic Acid purifying process | |
| CN108938805A (en) | Xiaoyanling Decoction and preparation method thereof | |
| CN114053333A (en) | Traditional Chinese medicine composition for treating rhinitis and preparation method and using method thereof | |
| CN115154514B (en) | Industrial-grade preparation method of traditional Chinese medicine composition for treating hemorrhoids and product thereof | |
| CN1324044C (en) | Ursolic acid extracting method from privet leaf | |
| CN114306406B (en) | Method for preparing effective components of groundsel and removing adrifolin | |
| CN114425058B (en) | Traditional Chinese medicine composition for treating rhinitis and preparation method, medicine and preparation thereof | |
| CN115400060A (en) | Preparation process of gentian extract | |
| CN110025678A (en) | A kind of method of paeoniflorin content in raising KANGGAN JIAONANG | |
| CN111991503A (en) | Production process of snake gall and bulbus fritilariae liquid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |