CN115372630A - Method, device and medium for detecting peripherin in urine based on porous material - Google Patents
Method, device and medium for detecting peripherin in urine based on porous material Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
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Abstract
The invention relates to the field of artificial intelligence, and discloses a method, a device, electronic equipment and a storage medium for detecting peripherin in urine based on a porous material, wherein the method comprises the following steps: mixing the raw materials to obtain a mixed material, constructing a homogeneous material of the mixed material, emulsifying the homogeneous material to obtain an emulsified material, and performing solid-state conversion on the emulsified material; collecting urine to be detected, carrying out supernatant separation treatment on the urine to be detected to obtain separated supernatant, calculating the solution ratio of a solid material, and carrying out supernatant protein enrichment on the separated supernatant to obtain enriched supernatant protein; preparing a laser analysis auxiliary solution for enriching the supernatant protein, performing molecule-ion conversion on the enriched supernatant protein to obtain protein ions, and constructing a mass spectrum image of the protein ions; and performing peak value detection on the mass spectrum image, and determining the detection result of the peripherin in the urine to be detected. The invention can improve the detection sensitivity of the peripherin in urine.
Description
Technical Field
The invention relates to the field of artificial intelligence, in particular to a method and a device for detecting peripherin in urine based on a porous material, electronic equipment and a storage medium.
Background
The detection of the peripherin in the urine based on the porous material refers to a process of adsorbing a protein complex in the urine by using the porous material and detecting whether the adsorbed protein complex has the peripherin, and aims to detect whether abnormal protein exists in the urine.
At present, the detection methods for the proteins in the urine at this week include immuno-immobilization electrophoresis and immunoturbidimetry, wherein immuno-immobilization electrophoresis refers to a process of performing zone electrophoresis on a protein mixture on a solid phase carrier and then reacting with a specific antibody to detect a corresponding antigen combined with the antibody, and the process needs to perform specific antibody reaction on the urine with other materials to detect whether the proteins at this week exist, but the reaction result is usually not single and the reaction time is long; the immunoturbidimetry is a process that an antigen and an antibody react in a specific dilution system to form a soluble immune complex in a proper proportion and are precipitated from a liquid state to form particles under the action of a coagulant in the dilution system. Therefore, the detection sensitivity of the present week protein in urine is insufficient.
Disclosure of Invention
In order to solve the problems, the invention provides a method, a device, an electronic device and a storage medium for detecting the peripherin in the urine based on a porous material, which can improve the detection sensitivity of the peripherin in the urine.
In a first aspect, the present invention provides a method for detecting peripherin in urine based on a porous material, comprising:
obtaining an original material, mixing the original material to obtain a mixed material, constructing a homogeneous material of the mixed material, emulsifying the homogeneous material to obtain an emulsified material, and performing solid-state conversion on the emulsified material to obtain a solid material;
collecting urine to be detected, carrying out supernatant separation treatment on the urine to be detected to obtain separated supernatant, calculating the solution ratio of the solid material, and carrying out supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain enriched supernatant protein;
preparing the laser analysis auxiliary solution for enriching the supernatant protein, performing molecule-ion conversion on the enriched supernatant protein according to the laser analysis auxiliary solution to obtain protein ions, and constructing a mass spectrum image of the protein ions;
and performing peak detection on the mass spectrum image to obtain a detection peak value, and determining a detection result of the peripherin in the urine to be detected according to the detection peak value.
In a possible implementation manner of the first aspect, the performing material mixing on the raw materials to obtain a mixed material includes:
inquiring the mixing concentration and the mixing capacity of the original material;
calculating the material usage of the original material according to the mixing concentration and the mixing capacity;
mixing and dissolving the original material according to the material dosage to obtain a dissolved material;
performing liquid-mass conversion on the dissolved material to obtain the mixed material, wherein the material usage amount of the original material is calculated by using the following formula:
wherein,
represents the material usage of the original material,
the concentration of the mixture is expressed as,
representing the mixing capacity.
In one possible implementation manner of the first aspect, the constructing the homogeneous material of the mixed material includes:
preparing a constant-stability material of the mixed material;
constructing a temperature environment of the mixed material;
according to the temperature environment, performing material stabilization on the mixed material by using the constant-stability material to obtain a stabilized material;
checking whether the stabilizing material meets a preset uniformity;
when the stabilizing material does not accord with the preset uniformity, returning to the temperature environment, and utilizing the constant-stability material to stabilize the mixed material to obtain a stabilizing material;
and when the stabilizing material meets the preset uniformity, taking the stabilizing material as a homogeneous material of the mixed material.
In one possible implementation manner of the first aspect, the solid-state converting the emulsified material to obtain a solid-state material includes:
carrying out material solidification on the emulsified material to obtain a solidified material;
purifying the solidified material by using a hydrothermal method to obtain a purified material;
configuring a curing temperature of the purification material;
and carrying out solid-state conversion on the purified material according to the curing temperature to obtain the solid material.
In a possible implementation manner of the first aspect, the performing supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain an enriched supernatant protein includes:
obtaining a solid material corresponding to the solution ratio, and performing solution dissolution on the solid material by using sterile water to obtain a dissolved material;
preparing the mixing speed and the mixing temperature of the separated supernatant;
uniformly mixing the separated supernatant solution by using the dissolving material according to the uniformly mixing speed and the uniformly mixing temperature to obtain uniformly mixed supernatant;
carrying out protein enrichment centrifugation treatment on the uniformly mixed supernatant to obtain centrifugal enriched protein;
and (4) carrying out journal cleaning treatment on the centrifugal enriched protein to obtain the enriched supernatant protein.
In one possible implementation manner of the first aspect, the constructing a mass spectrum image of the protein ions includes:
obtaining supernatant protein corresponding to the protein ions, and inquiring the protein quality of the supernatant protein;
calculating the mass-to-charge ratio of the protein ions according to the mass of the protein by using the following formula:
wherein,
represents the mass-to-charge ratio of the protein ions,
represents the protein mass of the protein ion,
represents the charge carried by the protein ion;
determining a mass spectral image of the protein ions from the mass of the protein to the mass-to-charge ratio of the protein ions.
In a possible implementation manner of the first aspect, the performing peak detection on the mass spectrum image to obtain a detected peak includes:
inquiring the protein quantity value and mass-to-charge ratio scale of the protein ions in the mass spectrum image;
calculating a protein difference value of the protein quantity value according to the mass-to-charge ratio scale by using the following formula:
wherein,
a protein difference value representing the amount of said protein,
representing an ith of the mass-to-charge ratio scales,
a protein metric representing the protein ion;
comparing the protein difference value with a preset difference value in size;
and when the protein difference value is not smaller than the preset difference value, inquiring subsequent values in the protein value, and taking the subsequent values as the detection peak value.
In a second aspect, the present invention provides a device for detecting peripherin in urine based on a porous material, said device comprising:
the material solid-state conversion module is used for obtaining an original material, mixing the original material to obtain a mixed material, constructing a homogeneous material of the mixed material, emulsifying the homogeneous material to obtain an emulsified material, and performing solid-state conversion on the emulsified material to obtain a solid material;
the clear liquid protein enrichment module is used for collecting urine to be detected, carrying out supernatant separation treatment on the urine to be detected to obtain separated supernatant, calculating the solution ratio of the solid material, and carrying out supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain enriched supernatant protein;
the mass spectrum image construction module is used for configuring the laser analysis auxiliary solution for enriching the supernatant protein, performing molecule-ion conversion on the enriched supernatant protein according to the laser analysis auxiliary solution to obtain protein ions, and constructing a mass spectrum image of the protein ions;
and the detection result determining module is used for carrying out peak detection on the mass spectrum image to obtain a detection peak value, and determining the detection result of the peripherin in the urine to be detected according to the detection peak value.
In a third aspect, the present invention provides an electronic device comprising:
at least one processor; and a memory communicatively coupled to the at least one processor;
wherein the memory stores a computer program executable by the at least one processor to enable the at least one processor to perform a method of detecting a circumferential protein in urine based on a porous material as defined in any one of the above first aspects.
In a fourth aspect, the present invention provides a computer-readable storage medium storing a computer program, which when executed by a processor, implements the method for detecting the peripherin in urine based on a porous material as described in any one of the above first aspects.
Compared with the prior art, the technical principle and the beneficial effects of the scheme are as follows:
the embodiment of the present invention provides a method for preparing a material capable of extracting protein substances from urine by using the original material, further provides a method for preparing a material capable of extracting protein substances from urine by using the original material, provides a solution for extracting chemical substances by using the original material, provides a method for converting an inhomogeneous and unstable mixed material into a homogeneous material by mixing materials, and avoids adverse effects of unstable chemical substances on subsequent chemical reactions, provides a method for converting a homogeneous material into a homogeneous and stable material by using a homogeneous material for extracting chemical substances from urine, provides a method for converting a homogeneous material in a liquid state into a material capable of solidifying into a solid by using a solid material, provides a method for preparing a mixture by using a liquid material, provides a method for separating protein substances from urine by using a supernatant, provides a method for separating protein substances from urine, provides a supernatant fluid by using a supernatant, and provides a method for detecting whether protein substances are present in a serum solution, and provides a serum solution for separating protein substances from urine to be separated into a serum solution by using a serum, and a serum solution, further, the embodiment of the present invention provides a buffer protection effect for the enriched supernatant protein by configuring the laser desorption auxiliary solution for attaching the laser desorption auxiliary solution to the surface of the enriched supernatant protein, so as to ensure that the laser irradiated subsequently is absorbed by the laser desorption auxiliary solution first, and then the absorbed light beam is transmitted to the enriched supernatant protein, further, the embodiment of the present invention performs molecule-ion conversion for the enriched supernatant protein according to the laser desorption auxiliary solution, so as to convert chemical substances into ionized substances, and displays the detection result by using an electronic technology, so as to improve the efficiency of abnormal protein detection, further, the embodiment of the present invention improves the sensitivity of abnormal protein detection by constructing a mass spectrum image of the protein ions, so as to represent the detection result by an image, the embodiment of the present invention performs peak detection for the mass spectrum image, so as to detect abnormal data from the mass spectrum image, uses the abnormal data as a sign of abnormal protein, and further, the embodiment of the present invention determines the sensitivity of the urine detection according to the present detection peak, so as to determine whether abnormal protein occurs according to the detected by the present detection result of the detected protein, so as to quickly determine whether abnormal protein occurs according to the present detection result of the abnormal protein. Therefore, the method, the device, the electronic equipment and the storage medium for detecting the peripherin in the urine based on the porous material, which are provided by the embodiment of the invention, can improve the sensitivity of the detection of the peripherin.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and together with the description, serve to explain the principles of the invention.
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive exercise.
FIG. 1 is a schematic flow chart of a method for detecting peripherin in urine based on a porous material according to an embodiment of the present invention;
FIG. 2 is a schematic flow chart illustrating one of the steps of the method for detecting cyclin in urine provided in FIG. 1 according to an embodiment of the present invention;
FIG. 3 is a schematic flow chart illustrating another step of the method for detecting peripherin in urine based on porous material provided in FIG. 1 according to an embodiment of the present invention;
FIG. 4 is a schematic block diagram of a device for detecting peripherin in urine based on porous material according to an embodiment of the present invention;
fig. 5 is a schematic view of an internal structure of an electronic device for implementing a method for detecting a peripherin in urine based on a porous material according to an embodiment of the present invention.
Detailed Description
It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are given by way of illustration only.
The embodiment of the invention provides a method for detecting peripherin in urine based on a porous material, and the execution main body of the method for detecting the peripherin in urine based on the porous material comprises but is not limited to at least one of a server, a terminal and other electronic devices which can be configured to execute the method provided by the embodiment of the invention. In other words, the method for detecting the circumferential protein in urine based on the porous material can be performed by software or hardware installed in a terminal device or a server device, and the software can be a block chain platform. The server includes but is not limited to: a single server, a server cluster, a cloud server or a cloud server cluster, and the like. The server may be an independent server, or may be a cloud server that provides basic cloud computing services such as a cloud service, a cloud database, cloud computing, a cloud function, cloud storage, a Network service, cloud communication, a middleware service, a domain name service, a security service, a Content Delivery Network (CDN), a big data and artificial intelligence platform, and the like.
Fig. 1 is a schematic flow chart of a method for detecting peripherin in urine based on a porous material according to an embodiment of the present invention. The method for detecting the cyclin in urine based on the porous material, which is described in figure 1, comprises the following steps:
s1, obtaining an original material, mixing the original material to obtain a mixed material, constructing a homogeneous material of the mixed material, emulsifying the homogeneous material to obtain an emulsified material, and performing solid-state conversion on the emulsified material to obtain a solid material.
The embodiment of the invention is used for preparing the material capable of extracting the protein substances in the urine by obtaining the raw material. The raw materials are sodium acetate and acetic acid, the sodium acetate and the acetic acid can be used for preparing a porous material, the porous material is an organic complex polymer, has a three-dimensional pore structure, generally takes metal ions as connecting points, and is supported by organic ligands to form space 3D extension, so that the porous material is widely applied to catalysis, energy storage and separation, has high adsorption performance in the aspect of gas adsorption separation, can be used for adsorption separation of hydrogen storage and toxic and harmful gases, and is applied to catalysis.
Further, the embodiment of the invention can ensure that new chemical substances are generated subsequently for extracting the chemical substances by mixing the raw materials to realize the chemical reaction of the materials under the action of mixing the raw materials.
In an embodiment of the present invention, the mixing the raw materials to obtain a mixed material includes: inquiring the mixing concentration and the mixing capacity of the original material; calculating the material usage of the original material according to the mixing concentration and the mixing capacity; mixing and dissolving the original material according to the material dosage to obtain a dissolved material; performing liquid-mass conversion on the dissolved material to obtain the mixed material, wherein the material usage amount of the original material is calculated by using the following formula:
wherein,
represents the material usage of the original material,
the concentration of the mixture is expressed as,
representing the mixing capacity.
Optionally, the raw materials are mixed and dissolved according to the material dosage to obtain a dissolved material, and the dissolved material is obtained by dissolving 0.5298g of sodium acetate and 0.2085ml of acetic acid in a beaker by adding a small amount of deionized water; and performing liquid-to-mass conversion on the dissolved material to obtain the mixed material, and transferring the dissolved material into a 500ml volumetric flask, and adding water to a constant volume of 500ml to realize the constant volume.
Further, embodiments of the present invention avoid the adverse effect of unstable chemicals on subsequent chemical reactions by constructing a homogeneous material of the mixed material for converting the inhomogeneous and unstable mixed material into a homogeneous and stable material. Wherein, the homogeneous material refers to a uniform and stable solution material formed by mixing two or more than two substances.
In an embodiment of the present invention, the homogeneous material for constructing the hybrid material includes: preparing a constant-stability material of the mixed material; constructing a temperature environment of the mixed material; according to the temperature environment, performing material stabilization on the mixed material by using the constant-stability material to obtain a stabilized material; checking whether the stabilizing material meets a preset uniformity; when the stabilizing material does not accord with the preset uniformity, returning to the temperature environment, and utilizing the constant-stability material to stabilize the mixed material to obtain a stabilizing material; and when the stabilizing material meets the preset uniformity, taking the stabilizing material as a homogeneous material of the mixed material.
Optionally, said formulating a chemostat material of said mixed material is achieved by formulating 1g of gelatinous P123 (PEG-PPG-PEG, EO2OPO7OEO 2O) and 1.7g of sodium acetate; the temperature environment for constructing the mixed material is realized by constructing a temperature environment at 35 ℃; according to the temperature environment, the constant-stability material is utilized to perform material stabilization on the mixed material to obtain a stabilized material, and 1g of gelatinous P123 (PEG-PPG-PEG, EO2OPO7OEO 2O) and 1.7g of sodium acetate are added into 30ml of sodium acetate-acetic acid buffer solution and stirred at 35 ℃; the checking whether the stabilizing material meets the preset uniformity is carried out by checking whether the stirring time at 35 ℃ is greater than or equal to 3 hours.
Further, the embodiment of the invention performs emulsification treatment on the homogeneous phase material to convert the homogeneous phase material which is originally in a liquid state into a material which can be solidified into a solid, thereby ensuring convenience in subsequent proportioning by using the solid material.
In an embodiment of the present invention, the emulsifying the homogeneous material to obtain an emulsified material includes: preparing an emulsification auxiliary material and a temperature environment of the homogeneous phase material; and according to the temperature environment, mixing the emulsification auxiliary material and the homogeneous phase material to obtain the emulsification material.
Wherein the emulsification auxiliary material refers to TMSO and a silicon dioxide source, and is used for gelatinizing other substances.
Further, the embodiment of the invention performs solid-state conversion on the emulsified material to solidify the solidifiable emulsified material, thereby ensuring the convenience in subsequent proportioning by using the solid material.
In an embodiment of the present invention, referring to fig. 2, the performing solid state transformation on the emulsified material to obtain a solid state material includes:
s201, solidifying the emulsified material to obtain a solidified material;
s202, purifying the solidified material by using a hydrothermal method to obtain a purified material;
s203, configuring the curing temperature of the purification material;
and S204, carrying out solid-state conversion on the purified material according to the curing temperature to obtain the solid material.
The hydrothermal method refers to a method for preparing materials by dissolving and recrystallizing powder in a sealed pressure vessel by using water as a solvent. Optionally, the emulsifying material is subjected to material solidification, and the obtained solidified material is obtained by placing the mixture under static conditions.
S2, collecting urine to be detected, carrying out supernatant separation treatment on the urine to be detected to obtain separated supernatant, calculating the solution ratio of the solid material, and carrying out supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain enriched supernatant protein.
According to the embodiment of the invention, the urine to be detected is subjected to supernatant separation treatment so as to remove impurities in the urine to be detected, and a supernatant layer with a plasma component is extracted from the urine to be detected.
In an embodiment of the present invention, referring to fig. 3, the separating supernatant from the urine to be detected to obtain a separated supernatant includes:
s301, configuring the separation speed, the separation duration and the separation temperature of the urine to be detected;
s302, according to the separation speed, the separation duration and the separation temperature, carrying out supernatant separation treatment on the urine to be detected by utilizing a pre-configured high-speed centrifuge to obtain the separation supernatant.
Further, the embodiment of the present invention calculates the solution ratio of the solid material to design the required material dosage in advance.
In an embodiment of the present invention, the step of calculating the solution ratio of the solid material is similar to the principle of calculating the material usage amount of the raw material according to the mixing concentration and the mixing capacity, and is not further described herein.
Further, according to the embodiment of the invention, the supernatant protein of the separated supernatant is enriched according to the solution ratio, so that the solid material is used for adsorbing the protein complex in the separated supernatant, and whether abnormal substances exist in the protein complex in subsequent detection is ensured.
In an embodiment of the present invention, the performing supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain an enriched supernatant protein includes: obtaining a solid material corresponding to the solution ratio, and performing solution dissolution on the solid material by using sterile water to obtain a dissolved material; preparing the mixing speed and the mixing temperature of the separated supernatant; uniformly mixing the separated supernatant solution by using the dissolving material according to the uniformly mixing speed and the uniformly mixing temperature to obtain uniformly mixed supernatant; carrying out protein enrichment centrifugation treatment on the uniformly mixed supernatant to obtain centrifugal enriched protein; and (4) carrying out journal cleaning treatment on the centrifugally enriched protein to obtain the enriched supernatant protein.
Illustratively, the solution dissolution of the solid material with sterile water to obtain a dissolved material is carried out by dissolving the solid material in 1ml of sterile water; uniformly mixing the separated supernatant solution by using the dissolving material according to the uniformly mixing speed and the uniformly mixing temperature to obtain uniformly mixed supernatant, and uniformly mixing the dissolving material on a constant-temperature mixer at 850rpm for 2 hours at the temperature of 4 ℃; carrying out protein enrichment centrifugation on the uniformly mixed supernatant to obtain centrifugal enriched protein, centrifuging for 5 minutes at 15000rpm and 4 ℃, carefully sucking the supernatant, and reserving the precipitate to realize the purpose; and (3) carrying out journal cleaning treatment on the centrifugally enriched protein to obtain the enriched supernatant protein, and adding 1ml of deionized water to wash and precipitate for 1 time.
S3, preparing the laser analysis auxiliary solution for enriching the supernatant protein, performing molecule-ion conversion on the enriched supernatant protein according to the laser analysis auxiliary solution to obtain protein ions, and constructing a mass spectrum image of the protein ions.
According to the embodiment of the invention, the laser analysis auxiliary solution for enriching the supernatant protein is configured to be attached to the surface of the supernatant protein by using the laser analysis auxiliary solution, so that the laser irradiated subsequently is ensured to be firstly absorbed by the laser analysis auxiliary solution, and then the absorbed light beam is transmitted to the supernatant protein enrichment, thereby playing a role in buffering and protecting the supernatant protein enrichment. Wherein the laser analysis auxiliary solution refers to a matrix solution, such as ferulic acid matrix solution.
In an embodiment of the invention, the configuring the laser desorption auxiliary solution for enriching the supernatant protein includes: identifying a planar area of the enriched supernatant protein; constructing an auxiliary area of the enriched supernatant protein according to the plane area; preparing the auxiliary dosage of the enriched supernatant protein according to the attachment area; and preparing the laser analysis auxiliary solution for enriching the supernatant protein according to the auxiliary dosage.
Wherein the planar area refers to the area of the head top surface of the container in which the enriched supernatant protein is spread, and the auxiliary area refers to the area of the surface of the container in which the laser desorption auxiliary solution can completely cover the enriched supernatant protein.
Furthermore, according to the embodiment of the invention, the enriched supernatant protein is subjected to molecule-ion conversion according to the laser analysis auxiliary solution so as to be used for converting chemical substances into ionic substances, and the detection result is displayed by using an electronic technology, so that the efficiency of abnormal protein detection is improved.
In an embodiment of the present invention, the performing molecule-ion conversion on the enriched supernatant protein according to the laser desorption auxiliary solution to obtain protein ions includes: preparing the sample to be detected for enriching the supernatant protein according to the laser analysis auxiliary solution; and carrying out electronic removal treatment on the enriched supernatant protein by using a pulse laser beam to obtain the protein ions.
Further, the embodiment of the invention constructs the mass spectrum image of the protein ions to express the detection result through the image, so that the sensitivity of abnormal protein detection is improved. The mass spectrum image is an image formed by the use amount and the mass-to-charge ratio of protein ions, the use amount of the protein ions is generally expressed by ug, and is taken as the vertical axis of the mass spectrum image, and the mass-to-charge ratio is taken as the horizontal axis of the mass spectrum image.
In an embodiment of the present invention, the constructing a mass spectrum image of the protein ions includes: obtaining supernatant protein corresponding to the protein ions, and inquiring the protein quality of the supernatant protein; calculating the mass-to-charge ratio of the protein ions according to the mass of the protein by using the following formula:
wherein,
represents the mass-to-charge ratio of the protein ions,
represents the protein mass of the protein ions,
represents the charge carried by the protein ion;
determining a mass spectral image of the protein ions from the mass of the protein to the mass-to-charge ratio of the protein ions.
And S4, performing peak detection on the mass spectrum image to obtain a detection peak value, and determining the detection result of the peripherin in the urine to be detected according to the detection peak value.
According to the embodiment of the invention, the mass spectrum image is subjected to peak detection so as to be used for detecting abnormal data from the mass spectrum image, and the abnormal data is taken as a mark of abnormal protein.
In an embodiment of the present invention, the performing peak detection on the mass spectrum image to obtain a detected peak includes: inquiring the protein quantity value and mass-to-charge ratio scale of the protein ions in the mass spectrum image; calculating a protein difference value of the protein quantity value according to the mass-to-charge ratio scale by using the following formula:
wherein,
a protein difference value representing the amount of said protein,
representing an ith of the mass-to-charge ratio scales,
a protein metric representing the protein ion;
comparing the protein difference value with a preset difference value in size; and when the protein difference value is not smaller than the preset difference value, inquiring subsequent values in the protein value, and taking the subsequent values as the detection peak value.
Furthermore, according to the embodiment of the invention, the detection result of the peripherin in the urine to be detected is determined according to the detection peak value, so that the abnormal peripherin is determined according to the detected abnormal data, and thus whether the abnormal protein exists or not can be quickly judged, and the sensitivity of the peripherin detection is improved. The term "present week protein" refers to a free, abnormal immunoglobulin composed of light chains, usually present in the form of a dimer.
In an embodiment of the present invention, the determining the detection result of the peripherin in the urine to be detected according to the detection peak value includes: determining the peripherin mark and the peripherin value in the urine to be detected according to the detection peak value; and taking the peripherin mark and the peripherin quantity value as a detection result of the peripherin in the urine to be detected.
It can be seen that, in the embodiments of the present invention, by obtaining the original material for preparing the material capable of extracting the protein substance in the urine by using the original material, further, by mixing the original material for realizing the chemical reaction of the material under the action of mixing the material, it is ensured that new chemical substances are generated subsequently for extracting the chemical substances, further, by constructing the homogeneous material of the mixed material for converting the inhomogeneous and unstable mixed material into the homogeneous material which is homogeneous and stable, it is avoided that the unstable chemical substances adversely affect the subsequent chemical reaction, further, by performing emulsification treatment on the homogeneous material for converting the homogeneous material which is originally in a liquid state into a material capable of being solidified into a solid, the convenience in proportioning by using solid materials is guaranteed, the convenience in proportioning by using the solid materials is further guaranteed by carrying out solid-state conversion on the emulsified materials to solidify the emulsified materials which can be solidified, and the convenience in proportioning by using the solid materials is further guaranteed, the urine to be detected is subjected to supernatant separation treatment to remove impurities in the urine to be detected and extract a supernatant layer with a plasma component from the urine, the solution proportioning of the solid materials is calculated to design a required material using amount in advance, the supernatant protein enrichment is further carried out on the separated supernatant according to the solution proportioning to adsorb protein compounds in the separated supernatant by using the solid materials, whether abnormal substances exist in the protein complex or not is guaranteed to be detected subsequently, further, the laser analysis auxiliary solution for enriching the supernatant protein is configured to be attached to the surface of the protein complex, the laser irradiated subsequently is guaranteed to be absorbed by the laser analysis auxiliary solution firstly, and then the absorbed light beam is transmitted to the protein complex, so that the buffer protection effect on the protein complex is achieved. Therefore, the method for detecting the peripherin in the urine based on the porous material can improve the sensitivity of the detection of the peripherin.
FIG. 4 is a functional block diagram of the device for detecting the peripherin in urine based on porous material according to the present invention.
The present invention provides a device 400 for detecting the peripherin in urine based on porous material, which can be installed in an electronic device. According to the realized functions, the device for detecting the peripherin in the urine based on the porous material can comprise a material solid-state conversion module 401, a serum protein enrichment module 402, a mass spectrum image construction module 403 and a detection result determination module 404. The module of the present invention, which may also be referred to as a unit, refers to a series of computer program segments that can be executed by a processor of an electronic device and that can perform a fixed function, and that are stored in a memory of the electronic device.
In the embodiment of the present invention, the functions of the modules/units are as follows:
the material solid-state conversion module 401 is configured to obtain an original material, perform material mixing on the original material to obtain a mixed material, construct a homogeneous material of the mixed material, perform emulsification treatment on the homogeneous material to obtain an emulsified material, and perform solid-state conversion on the emulsified material to obtain a solid material;
the clear liquid protein enrichment module 402 is configured to collect urine to be detected, separate a supernatant of the urine to be detected to obtain a separated supernatant, calculate a solution ratio of the solid material, and enrich the supernatant of the separated supernatant according to the solution ratio to obtain an enriched supernatant protein;
the mass spectrum image construction module 403 is configured to configure the laser desorption auxiliary solution for enriching the supernatant protein, perform molecule-ion conversion on the enriched supernatant protein according to the laser desorption auxiliary solution to obtain protein ions, and construct a mass spectrum image of the protein ions;
the detection result determining module 404 is configured to perform peak detection on the mass spectrum image to obtain a detection peak value, and determine a detection result of the peripherin in the urine to be detected according to the detection peak value.
In detail, when the modules in the device 400 for detecting peripherin in urine based on porous material according to the embodiment of the present invention are used, the same technical means as the method for detecting peripherin in urine based on porous material described in fig. 1 to 3 is adopted, and the same technical effects can be produced, which is not described herein again.
Fig. 5 is a schematic structural diagram of an electronic device for implementing the method for detecting the peripherin in urine based on a porous material according to the present invention.
The electronic device may include a processor 50, a memory 51, a communication bus 52 and a communication interface 53, and may further include a computer program stored in the memory 51 and executable on the processor 50, such as a porous material-based urine peripherin detection program.
In some embodiments, the processor 50 may be composed of an integrated circuit, for example, a single packaged integrated circuit, or may be composed of a plurality of integrated circuits packaged with the same function or different functions, and includes one or more Central Processing Units (CPUs), microprocessors, digital Processing chips, graphics processors, and combinations of various control chips. The processor 50 is a Control Unit (Control Unit) of the electronic device, connects various components of the electronic device by using various interfaces and lines, and executes various functions and processes data of the electronic device by running or executing programs or modules stored in the memory 51 (for example, executing a circumferential protein detection program in urine based on a porous material, etc.), and calling data stored in the memory 51.
The memory 51 includes at least one type of readable storage medium including flash memory, removable hard disks, multimedia cards, card-type memory (e.g., SD or DX memory, etc.), magnetic memory, magnetic disks, optical disks, etc. The memory 51 may in some embodiments be an internal storage unit of the electronic device, for example a removable hard disk of the electronic device. The memory 51 may also be an external storage device of the electronic device in other embodiments, such as a plug-in mobile hard disk, a Smart Media Card (SMC), a Secure Digital (SD) Card, a Flash memory Card (Flash Card), and the like provided on the electronic device. Further, the memory 51 may also include both an internal storage unit and an external storage device of the electronic device. The memory 51 may be used to store not only application software installed in the electronic device and various types of data, such as codes of a database configuration connection program, but also temporarily store data that has been output or will be output.
The communication bus 52 may be a Peripheral Component Interconnect (PCI) bus, an Extended Industry Standard Architecture (EISA) bus, or the like. The bus may be divided into an address bus, a data bus, a control bus, etc. The bus is arranged to enable connection communication between the memory 51 and at least one processor 50 or the like.
The communication interface 53 is used for communication between the electronic device 5 and other devices, and includes a network interface and a user interface. Optionally, the network interface may include a wired interface and/or a wireless interface (e.g., WI-FI interface, bluetooth interface, etc.), which are typically used to establish a communication connection between the electronic device and other electronic devices. The user interface may be a Display (Display), an input unit, such as a Keyboard (Keyboard), and optionally a standard wired interface, a wireless interface. Alternatively, in some embodiments, the display may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, an OLED (Organic Light-Emitting Diode) touch device, or the like. The display, which may also be referred to as a display screen or display unit, is suitable, among other things, for displaying information processed in the electronic device and for displaying a visualized user interface.
Fig. 5 shows only an electronic device with components, and those skilled in the art will appreciate that the structure shown in fig. 5 does not constitute a limitation of the electronic device, and may include fewer or more components than shown, or some components may be combined, or a different arrangement of components.
For example, although not shown, the electronic device may further include a power supply (such as a battery) for supplying power to each component, and preferably, the power supply may be logically connected to the at least one processor 50 through a power management device, so that functions of charge management, discharge management, power consumption management and the like are realized through the power management device. The power supply may also include any component of one or more dc or ac power sources, recharging devices, power failure detection circuitry, power converters or inverters, power status indicators, and the like. The electronic device may further include various sensors, a bluetooth module, a Wi-Fi module, and the like, which are not described herein again.
It is to be understood that the embodiments described are for illustrative purposes only and that the scope of the claimed invention is not limited to this configuration.
The database configuration connection program stored in the memory 51 of the electronic device is a combination of a plurality of computer programs, and when running in the processor 50, can realize:
obtaining an original material, mixing the original material to obtain a mixed material, constructing a homogeneous material of the mixed material, emulsifying the homogeneous material to obtain an emulsified material, and performing solid-state conversion on the emulsified material to obtain a solid material;
collecting urine to be detected, carrying out supernatant separation treatment on the urine to be detected to obtain separated supernatant, calculating the solution ratio of the solid material, and carrying out supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain enriched supernatant protein;
preparing the laser analysis auxiliary solution for enriching the supernatant protein, and performing molecule-ion conversion on the enriched supernatant protein according to the laser analysis auxiliary solution to obtain protein ions and construct a mass spectrum image of the protein ions;
and performing peak detection on the mass spectrum image to obtain a detection peak value, and determining a detection result of the peripherin in the urine to be detected according to the detection peak value.
Specifically, the processor 50 may refer to the description of the relevant steps in the embodiment corresponding to fig. 1 for a specific implementation method of the computer program, which is not described herein again.
Further, the electronic device integrated module/unit, if implemented in the form of a software functional unit and sold or used as a separate product, may be stored in a non-volatile computer-readable storage medium. The storage medium may be volatile or nonvolatile. For example, the computer-readable medium may include: any entity or device capable of carrying said computer program code, a recording medium, a usb-disk, a removable hard disk, a magnetic diskette, an optical disk, a computer Memory, a Read-Only Memory (ROM).
The present invention also provides a storage medium, which is readable and stores a computer program that, when executed by a processor of an electronic device, can implement:
obtaining an original material, mixing the original material to obtain a mixed material, constructing a homogeneous material of the mixed material, emulsifying the homogeneous material to obtain an emulsified material, and performing solid-state conversion on the emulsified material to obtain a solid material;
collecting urine to be detected, carrying out supernatant separation treatment on the urine to be detected to obtain separated supernatant, calculating the solution ratio of the solid material, and carrying out supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain enriched supernatant protein;
preparing the laser analysis auxiliary solution for enriching the supernatant protein, performing molecule-ion conversion on the enriched supernatant protein according to the laser analysis auxiliary solution to obtain protein ions, and constructing a mass spectrum image of the protein ions;
and performing peak detection on the mass spectrum image to obtain a detection peak value, and determining a detection result of the peripherin in the urine to be detected according to the detection peak value.
In the embodiments provided in the present invention, it should be understood that the disclosed apparatus, device and method can be implemented in other ways. For example, the above-described apparatus embodiments are merely illustrative, and for example, the division of the modules is only one logical functional division, and other divisions may be realized in practice.
The modules described as separate parts may or may not be physically separate, and parts displayed as modules may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, functional modules in the embodiments of the present invention may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit. The integrated unit can be realized in a form of hardware, or in a form of hardware plus a software functional module.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof.
The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference signs in the claims shall not be construed as limiting the claim concerned.
It is noted that, in this document, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrases "comprising a," "8230," "8230," or "comprising" does not exclude the presence of additional like elements in a process, method, article, or apparatus that comprises the element.
The foregoing are merely exemplary embodiments of the present invention, which enable those skilled in the art to understand or practice the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A method for detecting the peripherin in urine based on a porous material, which is characterized by comprising the following steps:
obtaining an original material, mixing the original material to obtain a mixed material, constructing a homogeneous material of the mixed material, emulsifying the homogeneous material to obtain an emulsified material, and performing solid-state conversion on the emulsified material to obtain a solid material;
collecting urine to be detected, carrying out supernatant separation treatment on the urine to be detected to obtain separated supernatant, calculating the solution ratio of the solid material, and carrying out supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain enriched supernatant protein;
preparing the laser analysis auxiliary solution for enriching the supernatant protein, performing molecule-ion conversion on the enriched supernatant protein according to the laser analysis auxiliary solution to obtain protein ions, and constructing a mass spectrum image of the protein ions;
and performing peak detection on the mass spectrum image to obtain a detection peak value, and determining a detection result of the peripherin in the urine to be detected according to the detection peak value.
2. The method of claim 1, wherein said material mixing said starting materials to obtain a mixed material comprises:
inquiring the mixing concentration and the mixing capacity of the original materials;
calculating the material usage of the original material according to the mixing concentration and the mixing capacity;
mixing and dissolving the original material according to the material dosage to obtain a dissolved material;
performing liquid-mass conversion on the dissolved material to obtain the mixed material, wherein the material usage amount of the original material is calculated by using the following formula:
wherein,
represents the material usage of the original material,
the concentration of the mixture is expressed by the following formula,
representing the mixing capacity.
3. The method of claim 1, wherein the constructing a homogeneous material of the hybrid material comprises:
preparing a stabilizing material of the mixed material;
constructing a temperature environment of the mixed material;
according to the temperature environment, performing material stabilization on the mixed material by using the constant-stability material to obtain a stabilized material;
checking whether the stabilizing material meets a preset uniformity;
when the stabilizing material does not accord with the preset uniformity, returning to the temperature environment, and utilizing the constant-stability material to stabilize the mixed material to obtain a stabilizing material;
and when the stabilizing material meets the preset uniformity, taking the stabilizing material as a homogeneous material of the mixed material.
4. The method of claim 1, wherein said solid state converting said emulsified material to obtain a solid state material comprises:
carrying out material solidification on the emulsified material to obtain a solidified material;
purifying the solidified material by using a hydrothermal method to obtain a purified material;
configuring a curing temperature of the purification material;
and carrying out solid-state conversion on the purified material according to the curing temperature to obtain the solid material.
5. The method according to claim 1, wherein the supernatant protein enrichment of the separated supernatant according to the solution ratio is performed to obtain an enriched supernatant protein, and the method comprises:
obtaining a solid material corresponding to the solution ratio, and performing solution dissolution on the solid material by using sterile water to obtain a dissolved material;
preparing the mixing speed and the mixing temperature of the separated supernatant;
uniformly mixing the separated supernatant solution by using the dissolving material according to the uniformly mixing speed and the uniformly mixing temperature to obtain uniformly mixed supernatant;
carrying out protein enrichment centrifugation treatment on the uniformly mixed supernatant to obtain centrifugal enriched protein;
and (4) carrying out journal cleaning treatment on the centrifugally enriched protein to obtain the enriched supernatant protein.
6. The method of claim 1, wherein constructing a mass spectral image of the protein ions comprises:
obtaining supernatant protein corresponding to the protein ions, and inquiring the protein quality of the supernatant protein;
calculating the mass-to-charge ratio of the protein ions according to the mass of the protein by using the following formula:
wherein,
represents the mass-to-charge ratio of the protein ions,
represents the protein mass of the protein ions,
represents the charge carried by the protein ion;
determining a mass spectral image of the protein ions from the mass of the protein to the mass-to-charge ratio of the protein ions.
7. The method of claim 1, wherein the performing peak detection on the mass spectrum image to obtain a detected peak value comprises:
inquiring the protein quantity value and mass-to-charge ratio scale of the protein ions in the mass spectrum image;
calculating a protein difference value of the protein quantity value according to the mass-to-charge ratio scale by using the following formula:
wherein,
a protein difference value representing the amount of said protein,
representing an ith of the mass-to-charge ratio scales,
a protein metric representing the protein ion;
comparing the protein difference value with a preset difference value in size;
when the protein difference is not less than the preset difference, querying a subsequent quantity value in the protein quantity values, and taking the subsequent quantity value as the detection peak value.
8. A method and a device for detecting the peripherin in urine based on a porous material are characterized in that the device comprises:
the material solid-state conversion module is used for obtaining an original material, mixing the original material to obtain a mixed material, constructing a homogeneous material of the mixed material, emulsifying the homogeneous material to obtain an emulsified material, and performing solid-state conversion on the emulsified material to obtain a solid material;
the clear liquid protein enrichment module is used for collecting urine to be detected, carrying out supernatant separation treatment on the urine to be detected to obtain separated supernatant, calculating the solution ratio of the solid material, and carrying out supernatant protein enrichment on the separated supernatant according to the solution ratio to obtain enriched supernatant protein;
the mass spectrum image construction module is used for configuring the laser analysis auxiliary solution for enriching the supernatant protein, performing molecule-ion conversion on the enriched supernatant protein according to the laser analysis auxiliary solution to obtain protein ions, and constructing a mass spectrum image of the protein ions;
and the detection result determining module is used for carrying out peak detection on the mass spectrum image to obtain a detection peak value, and determining the detection result of the peripherin in the urine to be detected according to the detection peak value.
9. An electronic device, characterized in that the electronic device comprises:
at least one processor; and a memory communicatively coupled to the at least one processor; wherein the memory stores a computer program executable by the at least one processor to enable the at least one processor to perform the method of detecting the peripherin in urine based on a porous material according to any one of claims 1 to 7.
10. A computer-readable storage medium storing a computer program, wherein the computer program, when executed by a processor, implements the method for detecting the circumferential protein in urine based on a porous material according to any one of claims 1 to 7.
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