CN115346604A - DNA sample equilibrium analysis method and device - Google Patents
DNA sample equilibrium analysis method and device Download PDFInfo
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- CN115346604A CN115346604A CN202211283642.8A CN202211283642A CN115346604A CN 115346604 A CN115346604 A CN 115346604A CN 202211283642 A CN202211283642 A CN 202211283642A CN 115346604 A CN115346604 A CN 115346604A
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
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- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F16/00—Information retrieval; Database structures therefor; File system structures therefor
- G06F16/20—Information retrieval; Database structures therefor; File system structures therefor of structured data, e.g. relational data
- G06F16/22—Indexing; Data structures therefor; Storage structures
- G06F16/2282—Tablespace storage structures; Management thereof
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/30—Detection of binding sites or motifs
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
Abstract
The embodiment of the invention provides a method and a device for analyzing DNA sample equilibrium, wherein the method comprises the following steps: determining the type of the kit; introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identifier, a color group identifier, a locus name, a locus typing value, and a peak height of the locus typing value; calculating the average peak height of the gene locus according to the peak height of the gene locus typing value corresponding to the gene locus name; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result. The embodiment of the invention realizes the automatic analysis of the DNA sample balance, can assist the manual work to efficiently and accurately complete part of repetitive work in production or quality inspection work, and can reduce repetitive errors caused by human factors, thereby improving the production or quality inspection work efficiency and reducing unnecessary loss and risk.
Description
Technical Field
The embodiment of the invention relates to the technical field of computers and life sciences, in particular to a method and a device for analyzing DNA sample equilibrium.
Background
At present, in the analysis of DNA sample equilibrium in the production and quality inspection links of products, the obtained data are manually calculated and compared through Excel tables, a large number of Excel tables are required to carry out self-carried function screening and unnecessary data items are deleted, various equilibrium indexes are manually calculated according to requirements, and the next production or inspection is carried out. The process is very complicated, time-consuming, poor in operation experience, and has a certain degree of human error, which causes production failure and unnecessary loss, or quality inspection failure, which causes defective goods to flow into the market.
In order to reduce the error of manual calculation with manual repeatability and reduce the production error or quality inspection error caused by human error, an automated method is urgently needed to replace the manual method for performing DNA sample equalization analysis, thereby reducing unnecessary loss and the possibility of defective products entering the market and improving the efficiency and quality of production quality inspection.
Disclosure of Invention
Aiming at the defects in the prior art, the embodiment of the invention provides a DNA sample equilibrium analysis method and a DNA sample equilibrium analysis device.
The embodiment of the invention provides a DNA sample equilibrium analysis method, which comprises the following steps: determining the type of the kit; introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identity, a color group identity, a locus name, a locus typing value, and a peak height of the locus typing value; calculating the average peak height of the gene loci according to the peak height of the typing value of the gene loci corresponding to the name of the gene loci; and carrying out DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the DNA sample equilibrium analysis result comprises a locus equilibrium analysis result; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the DNA sample equilibrium analysis result comprises the following steps: obtaining the locus names having 2 of the locus typing values; obtaining a minimum height and a maximum height among peak heights of the 2 locus typing values; and calculating a first ratio of the minimum height to the maximum height, and taking the first ratio as a locus equilibrium analysis result corresponding to the locus name.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the DNA sample equilibrium analysis result comprises an intra-group equilibrium analysis result; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the DNA sample equilibrium analysis result comprises the following steps: obtaining the minimum value of the average peak height of the gene loci and the maximum value of the average peak height of the gene loci in the color group corresponding to each color group identification according to the color group identifications; and calculating a second ratio of the minimum value of the locus average peak heights to the maximum value of the locus average peak heights in the color group, and taking the second ratio as an in-group equilibrium analysis result of the color group.
According to the DNA sample balance analysis method provided by the embodiment of the invention, the DNA sample balance analysis result comprises an intergroup balance analysis result; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the analysis result comprises the following steps: acquiring the number of loci in the color group of the sample according to the sample identification, the color group identification and the locus name; calculating the average peak height of the color group according to the average peak height of the gene loci and the number of the gene loci in the color group; obtaining a color group maximum average peak height and a color group minimum average peak height according to the color group average peak heights of the color groups of the sample; and calculating a third ratio of the minimum average peak height of the color group to the maximum average peak height of the color group, and taking the third ratio as the analysis result of the balance among the groups.
According to an embodiment of the present invention, a method for analyzing DNA sample balance includes calculating a color group average peak height according to the locus average peak height and the number of loci in the color group, including: calculating the sum of the average peak heights of the loci of each locus in the color group to obtain the accumulated value of the average peak heights of the loci; dividing the accumulated value of the locus mean peak heights by the number of loci in the color group to obtain the color group mean peak height.
According to the method for analyzing the DNA sample balance, provided by the embodiment of the invention, the analysis result of the DNA sample balance comprises an overall balance analysis result; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the analysis result comprises the following steps: obtaining the minimum value of the average peak height of the gene locus and the maximum value of the average peak height of the gene locus in the corresponding sample according to the sample identification; and calculating a fourth ratio of the minimum value of the average peak height of the gene locus in the sample to the maximum value of the average peak height of the gene locus, and taking the fourth ratio as the overall equilibrium analysis result.
According to the method for analyzing the DNA sample balance provided by the embodiment of the invention, the method further comprises the following steps: displaying the DNA sample equilibrium analysis result and/or deriving the DNA sample equilibrium analysis result.
According to the method for analyzing the DNA sample balance, provided by the embodiment of the invention, the analysis result of the DNA sample balance comprises the analysis result of at least one preset balance index; the step of displaying the DNA sample equilibrium analysis result comprises the following steps: and comparing the analysis result of the preset balance index with the expected value of the corresponding preset balance index, and if the analysis result of the preset balance index is lower than the expected value of the corresponding preset balance index, highlighting the analysis result of the preset balance index.
The embodiment of the invention also provides a DNA sample equilibrium analysis device, which comprises: a determination module to: determining the type of the kit; an import module to: introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identification, a color group identification, a locus name, a locus typing value and a peak height of the locus typing value; a calculation module to: calculating the average peak height of the gene loci according to the peak height of the typing value of the gene loci corresponding to the name of the gene loci; an analysis module to: and carrying out DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
An embodiment of the present invention further provides an electronic device, which includes a memory, a processor, and a computer program stored in the memory and executable on the processor, where the processor implements the steps of any one of the methods for analyzing DNA sample equilibrium described above when executing the program.
Embodiments of the present invention further provide a non-transitory computer-readable storage medium, on which a computer program is stored, and the computer program, when executed by a processor, implements the steps of any of the methods for DNA sample equilibrium analysis described above.
An embodiment of the present invention further provides a computer program product, which includes a computer program, and when the computer program is executed by a processor, the steps of the DNA sample equilibrium analysis method as described in any one of the above are implemented.
According to the method and the device for analyzing the DNA sample balance, provided by the embodiment of the invention, the type of the kit is determined, the DNA sample data based on the type of the kit is introduced, the DNA sample data comprises the sample identification, the color group identification, the locus name, the locus typing value and the peak height of the locus typing value, the average peak height of the locus is calculated according to the peak height of the locus typing value corresponding to the locus name, the DNA sample balance analysis is carried out based on the DNA sample data and the average peak height of the locus, the analysis result of the DNA sample balance is obtained, the automatic analysis of the DNA sample balance is realized, the partial repeated work existing in the production or quality inspection work can be completed efficiently and accurately by manpower, meanwhile, the repeated errors caused by human factors can be reduced, the production or quality inspection work efficiency is improved, and unnecessary loss and risks are reduced.
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In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.
FIG. 1 is a schematic flow chart of a DNA sample equilibrium analysis method provided by an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a DNA sample equilibrium analysis apparatus according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of an electronic device according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
FIG. 1 is a schematic flow chart of a method for analyzing DNA sample equilibrium according to an embodiment of the present invention. As shown in fig. 1, the method includes:
and S1, determining the type of the kit.
Different types of kits collect different types of DNA samples because the types of sample data of DNA sample corresponding to different types of kits are different. DNA sample data collected from the same type of kit needs to be analyzed. Therefore, the kit type is first determined before the DNA sample data is subjected to the equilibrium analysis.
The equilibrium analysis of the DNA sample can be used as one of the items for detecting the quality of the components of the kit. The process of determining the type of kit may include: inputting project names, and selecting corresponding project types and kit types to create DNA production quality inspection projects; wherein, the item type is used for distinguishing quality inspection quality indexes, such as DNA sample equilibrium property inspection items.
For example, a human genome STR identification kit component production quality inspection project is newly established, project names are input, and corresponding project types and kit type creation projects are selected. In this embodiment, the item supports management operations such as opening and deleting.
S2, importing DNA sample data based on the kit type; wherein the DNA sample data comprises a sample identification, a color group identification, a locus name, a locus typing value and a peak height of the locus typing value.
And introducing DNA sample data based on the kit type, namely DNA sample data collected by the kit of the corresponding kit type. The DNA sample data includes a sample identity, a color group identity, a locus name, a locus typing value, and a peak height of the locus typing value. The sample identifier is used to uniquely identify a DNA sample (which may be simply referred to as a sample), and the sample identifier may be, for example, a sample name, a sample number, or the like. The kit performs sample collection by fluorescent dyes of different colors (or single color). The color group identifier is used to uniquely identify the color group, and may be represented by a name of a color, an english symbol, a letter, or the like. For example, blue is denoted by B and green is denoted by G. The locus names correspond to the loci one to one for identifying different loci. There are 1 to 2 locus typing values per locus. Locus typing values correspond to peak heights.
DNA detection data information (DNA sample data) may be obtained through a sample file imported by a user, and the import file type may be in the ". Csv" format. In this embodiment, a user can import several to several hundred thousand pieces of product detection data at a time, and can simultaneously analyze all the data imported at one time. After the csv file is imported, the backup file is also included in an item list corresponding to the software terminal.
The DNA sample data in the sample file is read and can be further stored into a database such as SQLite according to a standard format. The fields processed by the algorithm and stored in the sample raw data table of the database may include: ID (automatically generated serial number), project ID, file ID, color group identification, sample identification, locus name, locus typing value, and peak height.
And S3, calculating the average peak height of the loci according to the peak height of the locus typing value corresponding to the locus name.
After the original sample data is recorded into a database, the mean locus peak height of each locus is firstly calculated. The general calculation of the mean peak height of a locus is as follows: if the locus has two locus typing values, the sum of the peak heights of the two locus typing values is divided by 2 to be used as the locus average peak height of the locus; if a locus has only one locus typing value then the locus average peak height is determined by directly dividing the peak height of that locus typing value by 2 (since the peak height for a typing value is actually the superposition of two peak heights).
To be noted: there are special cases when finding the mean peak height of a locus, such as: a. the Y chromosome is calculated and balanced and the single peak does not need to be averaged, namely if the locus of the Y chromosome has only one locus typing value, the peak height of the locus typing value is directly taken as the average peak height of the locus and is not divided by 2; b. if the DYF404S1 isogenic locus of the Y chromosome is a single peak, the peak height is divided by 2 to obtain an average value, namely, if only one locus typing value exists in the DYF404S1 isogenic locus of the Y chromosome, the peak height of the locus typing value is divided by 2 to obtain the average peak height of the DYF404S1 locus.
As another example, in the X chromosome, since men and women have different numbers on the chromosome, they also need to be treated separately. When calculating female X chromosome balance, if the balance is double peak, adding the peak heights of the two locus typing values and dividing by 2 to be used as the locus average peak height of the locus; if it is a single peak, the locus average peak height is determined by dividing the locus typing value by 2. When the male X chromosome balance is calculated, the average value is not required to be taken, namely if the male X chromosome has only one locus typing value, the peak height of the locus typing value is directly taken as the average peak height of the locus and is not divided by 2.
This method includes, but is not limited to, average peak height acquisition of loci or sites such as autosomes, X chromosomes, Y chromosomes, mitochondrial DNA, SNPs, etc.
After the average locus peak height is calculated, the average locus peak height can be stored in a new table, the equilibrium height result data table. The table field may include: id. Item ID, color group identification, sample identification, locus name, number of locus typing values for a locus, and locus average peak height.
And S4, carrying out DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
Different DNA samples can be distinguished according to the sample identifications in the DNA sample data. Different color groups can be distinguished according to color group identification in the DNA sample data. According to the locus name, the locus typing value and the peak height of the locus typing value in the DNA sample data, different loci can be distinguished, and the peak height of the corresponding locus typing value is obtained.
And performing DNA sample equilibrium analysis according to a preset equilibrium index based on the DNA sample data and the calculated average peak height of the locus to obtain a DNA sample equilibrium analysis result.
According to the method for analyzing the DNA sample balance, the type of the kit is determined, the DNA sample data based on the type of the kit is introduced, the DNA sample data comprises the sample identification, the color group identification, the name of the gene locus, the typing value of the gene locus and the peak height of the typing value of the gene locus, the average peak height of the gene locus is calculated according to the peak height of the typing value of the gene locus corresponding to the name of the gene locus, the DNA sample balance analysis is carried out based on the DNA sample data and the average peak height of the gene locus, the result of the DNA sample balance analysis is obtained, the automatic analysis of the DNA sample balance is realized, the partial repetitive work existing in the production or quality inspection work can be effectively and accurately completed manually, meanwhile, the repetitive errors caused by human factors can be reduced, the working efficiency of the production or quality inspection is improved, and unnecessary loss and risks are reduced.
According to the method for analyzing the DNA sample balance, provided by the embodiment of the invention, the DNA sample balance analysis result comprises a locus balance analysis result; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the DNA sample equilibrium analysis result comprises the following steps: obtaining said locus names having 2 of said locus typing values; obtaining a minimum height and a maximum height among peak heights of the 2 locus typing values; and calculating a first ratio of the minimum height to the maximum height, and taking the first ratio as a locus equilibrium analysis result corresponding to the locus name.
The preset equilibrium index comprises locus equilibrium, and the DNA sample equilibrium analysis result comprises a locus equilibrium analysis result. Accordingly, when the DNA sample equilibrium analysis is performed based on the DNA sample data and the locus average peak height to obtain the DNA sample equilibrium analysis result, because only the locus equilibrium of the loci having 2 locus typing values needs to be considered, the original table data of the sample may be queried through the database, the locus names having 2 locus typing values are screened out, the minimum height and the maximum height among the peak heights of the locus typing values corresponding to the loci are queried, the first ratio of the minimum height and the maximum height is calculated, and the first ratio is used as the locus equilibrium analysis result corresponding to the locus name, that is, the locus equilibrium analysis result of the locus corresponding to the locus name.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the locus names with 2 locus typing values are obtained, the minimum height and the maximum height in the peak heights of the 2 locus typing values are obtained, the first ratio of the minimum height and the maximum height is calculated, and the first ratio is used as the locus equilibrium analysis result corresponding to the locus names, so that the automatic analysis of the locus equilibrium is realized.
According to the method for analyzing the DNA sample balance, provided by the embodiment of the invention, the analysis result of the DNA sample balance comprises an analysis result of the group balance; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the DNA sample equilibrium analysis result comprises the following steps: acquiring the minimum value of the average peak height of the gene locus and the maximum value of the average peak height of the gene locus in the color group corresponding to each color group mark according to the color group marks; and calculating a second ratio of the minimum value of the average peak height of the gene loci in the color group to the maximum value of the average peak height of the gene loci, and taking the second ratio as the analysis result of the intra-group equilibrium of the color group.
The predetermined balance index includes an intra-group balance, or an intra-color-group balance. The DNA sample equilibrium analysis results include intra-group equilibrium analysis results. Correspondingly, when the DNA sample equilibrium analysis is carried out based on the DNA sample data and the locus average peak height to obtain the DNA sample equilibrium analysis result, the equilibrium height result data table can be inquired through the database, the minimum value of the locus average peak height and the maximum value of the locus average peak height of each color group of each sample can be inquired according to the sample identification and the color group identification, the second ratio of the minimum value of the locus average peak height and the maximum value of the locus average peak height in the color group is calculated, and the second ratio is used as the intra-group equilibrium analysis result of the color group.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the minimum value of the locus average peak height and the maximum value of the locus average peak height in the color group corresponding to each color group identifier are obtained according to the color group identifiers, the second ratio of the minimum value of the locus average peak height and the maximum value of the locus average peak height in the color group is calculated, and the second ratio is used as the intra-group equilibrium analysis result of the color group, so that the automatic analysis of the intra-group equilibrium is realized.
According to the DNA sample balance analysis method provided by the embodiment of the invention, the DNA sample balance analysis result comprises an intergroup balance analysis result; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the analysis result comprises the following steps: acquiring the number of loci in the color group of the sample according to the sample identification, the color group identification and the locus name; calculating the average peak height of the color group according to the average peak height of the gene loci and the number of the gene loci in the color group; obtaining a color group maximum average peak height and a color group minimum average peak height according to the color group average peak heights of the color groups of the sample; and calculating a third ratio of the minimum average peak height of the color group to the maximum average peak height of the color group, and taking the third ratio as the analysis result of the balance among the groups.
The predetermined balance index includes the balance among groups, or color balance among groups. The DNA sample balance analysis result comprises a group balance analysis result. Accordingly, when the DNA sample equilibrium analysis is performed based on the DNA sample data and the locus average peak height to obtain the DNA sample equilibrium analysis result, the equilibrium height result data table may be queried through the database, the number of loci in the color group of the sample may be obtained according to the sample identifier, the color group identifier, and the locus name, and the color group average peak height may be calculated according to the locus average peak height and the number of loci of the loci in the color group, for example, the color group average peak height may be obtained by dividing the result of the weighted summation of the locus average peak heights of the loci in the color group by the number of loci. And aiming at a certain DNA sample to be analyzed, obtaining the maximum average peak height of the color group and the minimum average peak height of the color group according to the average peak height of the color group of each color group of the DNA sample. And calculating a third ratio of the minimum average peak height of the color group to the maximum average peak height of the color group, and taking the third ratio as the analysis result of the interclass equilibrium of the DNA sample.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the number of loci in a color group of a sample is obtained according to the sample identification, the color group identification and the locus name, the average peak height of the color group is calculated according to the average peak height of the loci and the number of the loci in the color group, the maximum average peak height of the color group and the minimum average peak height of the color group are obtained according to the average peak height of the color group of each color group of the sample, the third ratio of the minimum average peak height of the color group and the maximum average peak height of the color group is calculated, and the third ratio is used as an analysis result of the inter-group equilibrium, so that the automatic analysis of the inter-group equilibrium is realized.
According to an embodiment of the present invention, a method for analyzing DNA sample balance includes calculating a color group average peak height according to the locus average peak height and the number of loci in the color group, including: calculating the sum of the average peak heights of the loci of each locus in the color group to obtain the accumulated value of the average peak heights of the loci; dividing the accumulated value of the locus mean peak heights by the number of loci in the color group to obtain the color group mean peak heights.
When calculating the average peak height of the color group from the average peak height of loci and the number of loci in the color group, the average peak height of the color group can be obtained by directly averaging the average peak heights of loci in the color group. Specifically, calculating the sum of the locus average peak heights of each locus in the color group to obtain an accumulated value of the locus average peak heights; the color group mean peak height is obtained by dividing the accumulated value of locus mean peak heights by the number of loci in the color group.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the accumulated value of the average peak heights of the loci is obtained by calculating the sum of the average peak heights of the loci in the color group, and the average peak height of the color group is obtained by dividing the accumulated value of the average peak heights of the loci by the number of loci in the color group, so that the average peak height of the color group can be quickly, simply and conveniently obtained.
According to the method for analyzing the DNA sample balance, provided by the embodiment of the invention, the analysis result of the DNA sample balance comprises an overall balance analysis result; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the DNA sample equilibrium analysis result comprises the following steps: obtaining the minimum value of the average peak height of the gene locus and the maximum value of the average peak height of the gene locus in the corresponding sample according to the sample identification; and calculating a fourth ratio of the minimum value of the average peak height of the gene locus in the sample to the maximum value of the average peak height of the gene locus, and taking the fourth ratio as the overall equilibrium analysis result.
The preset balance index comprises overall balance, and the DNA sample balance analysis result comprises an overall balance analysis result. Correspondingly, when the DNA sample equilibrium analysis result is obtained by performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height, the equilibrium height result data table can be inquired through the database, the minimum value of the locus average peak height and the maximum value of the locus average peak height in the corresponding sample are obtained according to the sample identification, the fourth ratio of the minimum value of the locus average peak height and the maximum value of the locus average peak height in the sample is calculated, and the fourth ratio is used as the overall equilibrium analysis result.
It should be noted that "first", "second", "third", and "fourth" in the first ratio, the second ratio, the third ratio, and the fourth ratio are only used to distinguish different types of ratios.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the minimum value of the locus average peak height and the maximum value of the locus average peak height in the corresponding sample are obtained according to the sample identification, the fourth ratio of the minimum value of the locus average peak height and the maximum value of the locus average peak height in the sample is calculated, and the fourth ratio is used as the overall equilibrium analysis result, so that the automatic analysis of the overall equilibrium of the DNA sample is realized.
According to the method for analyzing the DNA sample balance provided by the embodiment of the invention, the method further comprises the following steps: displaying the DNA sample equilibrium analysis result and/or deriving the DNA sample equilibrium analysis result.
After the DNA sample equilibrium analysis result is obtained, the DNA sample equilibrium analysis result may be directly displayed on a display interface of the software, or the DNA sample equilibrium analysis result may be exported, for example, to a file in an Excel format.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the DNA sample equilibrium analysis result is displayed and/or derived, so that the DNA sample equilibrium analysis result is visualized.
According to the DNA sample equilibrium analysis method provided by the embodiment of the invention, the DNA sample equilibrium analysis result comprises an analysis result of at least one preset equilibrium index; the step of displaying the DNA sample equilibrium analysis result comprises the following steps: and comparing the analysis result of the preset balance index with the expected value of the corresponding preset balance index, and if the analysis result of the preset balance index is lower than the expected value of the corresponding preset balance index, highlighting the analysis result of the preset balance index.
The DNA sample equilibrium analysis result comprises an analysis result of at least one preset equilibrium index. When the DNA sample equilibrium analysis result is displayed, the analysis result of the preset equilibrium index may be compared with the expected value of the corresponding preset equilibrium index, and if the analysis result of the preset equilibrium index is lower than the expected value of the corresponding preset equilibrium index, the analysis result of the preset equilibrium index is highlighted, for example, displayed in red; and if the analysis result of the preset equalization index is higher than the expected value of the corresponding preset equalization index, normally displaying the analysis result of the preset equalization index.
According to the method for analyzing the DNA sample balance, provided by the embodiment of the invention, the analysis result of the preset balance index is compared with the expected value of the corresponding preset balance index, and if the analysis result of the preset balance index is lower than the expected value of the corresponding preset balance index, the analysis result of the preset balance index is highlighted, so that the abnormal index data can be conveniently checked.
It should be noted that, on the premise that logics or structures do not conflict with each other, a plurality of preferred embodiments provided in this embodiment may be freely combined, and the present invention is not limited to this.
The following describes a DNA sample equilibrium analyzing apparatus provided in an embodiment of the present invention, and the DNA sample equilibrium analyzing apparatus described below and the DNA sample equilibrium analyzing apparatus method described above can be referred to in correspondence with each other.
FIG. 2 is a schematic structural diagram of a DNA sample equilibrium analysis apparatus according to an embodiment of the present invention. As shown in fig. 2, the apparatus includes a determining module 10, an importing module 20, a calculating module 30, and an analyzing module 40, wherein: the determination module 10 is configured to: determining the type of the kit; the import module 20 is configured to: introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identification, a color group identification, a locus name, a locus typing value and a peak height of the locus typing value; the calculation module 30 is configured to: calculating the average peak height of the gene loci according to the peak height of the typing value of the gene loci corresponding to the name of the gene loci; the analysis module 40 is configured to: and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the kit type is determined, DNA sample data based on the kit type is introduced, the DNA sample data comprises the sample identification, the color group identification, the locus name, the locus typing value and the peak height of the locus typing value, the locus average peak height is calculated according to the peak height of the locus typing value corresponding to the locus name, the DNA sample equilibrium analysis is carried out based on the DNA sample data and the locus average peak height to obtain the DNA sample equilibrium analysis result, the automatic analysis of the DNA sample equilibrium is realized, the partial repetitive work existing in the production or quality inspection work can be effectively and accurately completed by manpower, meanwhile, the repetitive errors caused by human factors can be reduced, the production or quality inspection work efficiency is improved, and unnecessary loss and risks are reduced.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the DNA sample equilibrium analysis result comprises a locus equilibrium analysis result; when the analysis module 40 is configured to perform DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, it is specifically configured to: obtaining said locus names having 2 of said locus typing values; obtaining a minimum height and a maximum height among peak heights of the 2 locus typing values; and calculating a first ratio of the minimum height to the maximum height, and taking the first ratio as a locus equilibrium analysis result corresponding to the locus name.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the locus names with 2 locus typing values are obtained, the minimum height and the maximum height in the peak heights of the 2 locus typing values are obtained, the first ratio of the minimum height and the maximum height is calculated, and the first ratio is used as the locus equilibrium analysis result corresponding to the locus names, so that the automatic analysis of the locus equilibrium is realized.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the DNA sample equilibrium analysis result comprises an intra-group equilibrium analysis result; when the analysis module 40 is configured to perform DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, it is specifically configured to: obtaining the minimum value of the average peak height of the gene loci and the maximum value of the average peak height of the gene loci in the color group corresponding to each color group identification according to the color group identifications; and calculating a second ratio of the minimum value of the average peak height of the gene loci in the color group to the maximum value of the average peak height of the gene loci, and taking the second ratio as the analysis result of the intra-group equilibrium of the color group.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the minimum value of the locus average peak height and the maximum value of the locus average peak height in the color group corresponding to each color group identifier are obtained according to the color group identifiers, the second ratio of the minimum value of the locus average peak height and the maximum value of the locus average peak height in the color group is calculated, and the second ratio is used as the intra-group equilibrium analysis result of the color group, so that the automatic analysis of the intra-group equilibrium is realized.
According to the DNA sample balance analysis device provided by the embodiment of the invention, the DNA sample balance analysis result comprises an interclass balance analysis result; when the analysis module 40 is configured to perform DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, it is specifically configured to: acquiring the number of loci in the color group of the sample according to the sample identification, the color group identification and the locus name; calculating the average peak height of the color group according to the average peak height of the gene loci and the number of the gene loci in the color group; obtaining a color group maximum average peak height and a color group minimum average peak height according to the color group average peak height of each color group of the sample; and calculating a third ratio of the minimum average peak height of the color group to the maximum average peak height of the color group, and taking the third ratio as the analysis result of the balance among the groups.
The DNA sample equilibrium analysis apparatus provided in the embodiment of the present invention obtains the number of loci in the color group of the sample from the sample identifier, the color group identifier, and the locus name, calculates the color group average peak height from the locus average peak height and the number of loci of the loci in the color group, obtains the color group maximum average peak height and the color group minimum average peak height from the color group average peak height of each color group of the sample, calculates the third ratio of the color group minimum average peak height and the color group maximum average peak height, and uses the third ratio as the inter-group equilibrium analysis result, thereby realizing the automated analysis of the inter-group equilibrium.
According to an embodiment of the present invention, when the analyzing module 40 is configured to calculate the average peak height of the color set according to the average peak height of the loci and the number of loci of the loci in the color set, it is specifically configured to: calculating the sum of the locus average peak heights of each locus in the color group to obtain an accumulated value of the locus average peak heights; dividing the accumulated value of the locus mean peak heights by the number of loci in the color group to obtain the color group mean peak height.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the accumulated value of the locus average peak heights is obtained by calculating the sum of the locus average peak heights of all loci in the color group, and the color group average peak height is obtained by dividing the accumulated value of the locus average peak heights by the number of loci in the color group, so that the color group average peak heights can be quickly, simply and conveniently obtained.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the DNA sample equilibrium analysis result comprises an overall equilibrium analysis result; when the analysis module 40 is configured to perform DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, it is specifically configured to: obtaining the minimum value of the average peak height of the gene locus and the maximum value of the average peak height of the gene locus in the corresponding sample according to the sample identification; and calculating a fourth ratio of the minimum value of the average peak height of the gene locus in the sample to the maximum value of the average peak height of the gene locus, and taking the fourth ratio as the overall equilibrium analysis result.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the minimum value of the locus average peak height and the maximum value of the locus average peak height in the corresponding sample are obtained according to the sample identification, the fourth ratio of the minimum value of the locus average peak height and the maximum value of the locus average peak height in the sample is calculated, and the fourth ratio is used as the overall equilibrium analysis result, so that the automatic analysis of the overall equilibrium of the DNA sample is realized.
According to an embodiment of the present invention, there is provided a DNA sample equilibrium analyzing apparatus, the apparatus further including an output module, configured to: displaying the DNA sample equilibrium analysis result and/or deriving the DNA sample equilibrium analysis result.
The DNA sample equilibrium analysis device provided by the embodiment of the invention realizes the visualization of the DNA sample equilibrium analysis result by displaying and/or deriving the DNA sample equilibrium analysis result.
According to the DNA sample equilibrium analysis apparatus provided in the embodiment of the present invention, when the output module is used for displaying the DNA sample equilibrium analysis result, the output module is specifically configured to: and comparing the analysis result of the preset balance index with the expected value of the corresponding preset balance index, and if the analysis result of the preset balance index is lower than the expected value of the corresponding preset balance index, highlighting the analysis result of the preset balance index.
According to the DNA sample equilibrium analysis device provided by the embodiment of the invention, the analysis result of the preset equilibrium index is compared with the expected value of the corresponding preset equilibrium index, and if the analysis result of the preset equilibrium index is lower than the expected value of the corresponding preset equilibrium index, the analysis result of the preset equilibrium index is highlighted, so that abnormal data can be conveniently checked.
Fig. 3 is a schematic structural diagram of an electronic device according to an embodiment of the present invention, and as shown in fig. 3, the electronic device may include: a processor (processor) 310, a communication Interface (Communications Interface) 320, a memory (memory) 330 and a communication bus 340, wherein the processor 310, the communication Interface 320 and the memory 330 communicate with each other via the communication bus 340. The processor 310 may invoke logic instructions in the memory 330 to perform a method of DNA sample equality analysis, the method comprising: determining the type of the kit; introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identification, a color group identification, a locus name, a locus typing value and a peak height of the locus typing value; calculating the average peak height of the gene loci according to the peak height of the typing value of the gene loci corresponding to the name of the gene loci; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
In addition, the logic instructions in the memory 330 may be implemented in the form of software functional units and stored in a computer readable storage medium when the software functional units are sold or used as independent products. Based on such understanding, the technical solution of the present invention or a part thereof which substantially contributes to the prior art may be embodied in the form of a software product, which is stored in a storage medium and includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk, or an optical disk, and various media capable of storing program codes.
In another aspect, an embodiment of the present invention further provides a computer program product, where the computer program product includes a computer program, the computer program may be stored on a non-transitory computer-readable storage medium, and when the computer program is executed by a processor, a computer can execute the DNA sample equilibrium analysis method provided by the above methods, where the method includes: determining the type of the kit; introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identity, a color group identity, a locus name, a locus typing value, and a peak height of the locus typing value; calculating the average peak height of the gene loci according to the peak height of the typing value of the gene loci corresponding to the name of the gene loci; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
In another aspect, an embodiment of the present invention further provides a non-transitory computer-readable storage medium, on which a computer program is stored, where the computer program is implemented to perform the DNA sample equilibrium analyzing method provided by the foregoing methods when executed by a processor, and the method includes: determining the type of the kit; introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identity, a color group identity, a locus name, a locus typing value, and a peak height of the locus typing value; calculating the average peak height of the gene loci according to the peak height of the typing value of the gene loci corresponding to the name of the gene loci; and carrying out DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.
Through the above description of the embodiments, those skilled in the art will clearly understand that each embodiment may be implemented by software plus a necessary general hardware platform, and may also be implemented by hardware. Based on the understanding, the above technical solutions substantially or otherwise contributing to the prior art may be embodied in the form of a software product, which may be stored in a computer-readable storage medium, such as ROM/RAM, magnetic disk, optical disk, etc., and includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute the method according to the various embodiments or some parts of the embodiments.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (11)
1. A method for analyzing the equilibrium of a DNA sample, comprising:
determining the type of the kit;
introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identity, a color group identity, a locus name, a locus typing value, and a peak height of the locus typing value;
calculating the average peak height of the gene loci according to the peak height of the typing value of the gene loci corresponding to the name of the gene loci;
and carrying out DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
2. The method of claim 1, wherein the result of the DNA sample equilibrium analysis comprises a result of locus equilibrium analysis; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the analysis result comprises the following steps:
obtaining said locus names having 2 of said locus typing values;
obtaining a minimum height and a maximum height among peak heights of the 2 locus typing values;
and calculating a first ratio of the minimum height to the maximum height, and using the first ratio as the locus equilibrium analysis result corresponding to the locus name.
3. The method of claim 1, wherein the result of the DNA sample equilibrium analysis comprises a result of an intra-group equilibrium analysis; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the DNA sample equilibrium analysis result comprises the following steps:
acquiring the minimum value of the average peak height of the gene locus and the maximum value of the average peak height of the gene locus in the color group corresponding to each color group mark according to the color group marks;
and calculating a second ratio of the minimum value of the locus average peak heights to the maximum value of the locus average peak heights in the color group, and taking the second ratio as an in-group equilibrium analysis result of the color group.
4. The method according to claim 1, wherein the result of the DNA sample balance analysis comprises a result of an interclass balance analysis; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the analysis result comprises the following steps:
acquiring the number of loci in the color group of the sample according to the sample identification, the color group identification and the locus name;
calculating the average peak height of the color group according to the average peak height of the gene loci and the number of the gene loci in the color group;
obtaining a color group maximum average peak height and a color group minimum average peak height according to the color group average peak heights of the color groups of the sample;
and calculating a third ratio of the minimum average peak height of the color group to the maximum average peak height of the color group, and taking the third ratio as the analysis result of the balance among the groups.
5. The method for DNA sample equilibrium analysis according to claim 4, wherein the calculating color group average peak height according to the locus average peak height and the number of loci in the color group comprises:
calculating the sum of the locus average peak heights of each locus in the color group to obtain an accumulated value of the locus average peak heights;
dividing the accumulated value of the locus mean peak heights by the number of loci in the color group to obtain the color group mean peak height.
6. The method of claim 1, wherein the DNA sample balance analysis result comprises a global balance analysis result; and performing DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result, wherein the analysis result comprises the following steps:
obtaining the minimum value of the average peak height of the gene locus and the maximum value of the average peak height of the gene locus in the corresponding sample according to the sample identification;
and calculating a fourth ratio of the minimum value of the average peak height of the gene locus in the sample to the maximum value of the average peak height of the gene locus, and taking the fourth ratio as the overall equilibrium analysis result.
7. The method for DNA sample equilibrium analysis according to claim 1, characterized in that the method further comprises:
displaying the DNA sample equilibrium analysis result and/or deriving the DNA sample equilibrium analysis result.
8. The method for analyzing the DNA sample balance according to claim 7, wherein the analysis result of the DNA sample balance comprises an analysis result of at least one preset balance index; the step of displaying the DNA sample equilibrium analysis result comprises the following steps:
and comparing the analysis result of the preset equalization index with the expected value of the corresponding preset equalization index, and if the analysis result of the preset equalization index is lower than the expected value of the corresponding preset equalization index, highlighting the analysis result of the preset equalization index.
9. A DNA sample equilibrium analyzing apparatus, comprising:
a determination module to: determining the type of the kit;
an import module to: introducing DNA sample data based on the type of the kit; wherein the DNA sample data comprises a sample identification, a color group identification, a locus name, a locus typing value and a peak height of the locus typing value;
a calculation module to: calculating the average peak height of the gene loci according to the peak height of the typing value of the gene loci corresponding to the name of the gene loci;
an analysis module to: and carrying out DNA sample equilibrium analysis based on the DNA sample data and the locus average peak height to obtain a DNA sample equilibrium analysis result.
10. An electronic device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, wherein the processor when executing the program implements the steps of the DNA sample equilibrium analysis method according to any one of claims 1 to 8.
11. A non-transitory computer readable storage medium, on which a computer program is stored, wherein the computer program, when executed by a processor, implements the steps of the DNA sample equilibrium analysis method according to any one of claims 1 to 8.
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