CN115322945A - Method for improving conidium yield of pathogenic bacteria conidia of gummy stem blight of melon - Google Patents
Method for improving conidium yield of pathogenic bacteria conidia of gummy stem blight of melon Download PDFInfo
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- 244000052616 bacterial pathogen Species 0.000 title claims abstract description 64
- 241000219112 Cucumis Species 0.000 title claims abstract description 29
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 26
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 title claims abstract description 25
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000011591 potassium Substances 0.000 claims abstract description 46
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 46
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 42
- 239000008103 glucose Substances 0.000 claims abstract description 42
- 239000001963 growth medium Substances 0.000 claims abstract description 35
- 238000009630 liquid culture Methods 0.000 claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 claims abstract description 19
- 238000012258 culturing Methods 0.000 claims abstract description 11
- 244000061456 Solanum tuberosum Species 0.000 claims description 15
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 15
- 239000012153 distilled water Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 4
- 238000011282 treatment Methods 0.000 description 45
- 238000012360 testing method Methods 0.000 description 13
- 230000006698 induction Effects 0.000 description 12
- 230000028070 sporulation Effects 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
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- 239000000463 material Substances 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 238000013095 identification testing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000005080 plant death Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N3/00—Spore forming or isolating processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Abstract
The invention discloses a method for improving conidium spore yield of pathogenic bacteria of gummy stem blight of melon, relating to the technical field of biology, and the method comprises the following steps: inoculating pathogenic bacteria of gummy stem blight of melon into a liquid culture medium, and culturing to obtain conidia of the pathogenic bacteria of gummy stem blight of melon; wherein the liquid culture medium contains potassium fulvate. The method changes the content of glucose in the PD liquid culture medium for culturing the pathogenic bacteria of the gummy stem blight, determines the optimal dosage of the glucose, and on the basis, adds the potassium fulvate in the PD liquid culture medium, optimizes the culture temperature, and proves that the method can effectively improve the spore production rate and the spore production quantity of the conidia of the pathogenic bacteria of the gummy stem blight by observing the conidia production quantity of the pathogenic bacteria of the gummy stem blight.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a method for improving conidium spore yield of pathogenic bacteria of gummy stem blight of melons.
Background
The gummy stem blight of the melons is one of common soil-borne diseases in the cultivation of the melons, mainly harms leaves and stems, and leads to plant death and serious yield reduction when serious. In the research of gummy stem blight resistance of melons, the induction of conidium generation of pathogenic bacteria of gummy stem blight is a key point in a gummy stem blight backcross test and a breeding material resistance inoculation identification test. In a large number of researches, different types of strains of pathogenic bacteria of the gummy stem blight generate conidiospores greatly, and compared with other pathogenic bacteria, the pathogenic bacteria of the gummy stem blight are not easy to generate the conidiospores, so that the difficulty of researching the gummy stem blight resistance of the melon is increased to a certain extent. In the previous research, the main methods adopted for inducing the generation of conidia of pathogenic bacteria of gummy stem blight of melon include a sporulation induction method for inducing the conidia by dark culture and ultraviolet intermittent irradiation, a colony culture method (i.e., culturing bacterial colonies of pathogenic bacteria of gummy stem blight on a plate and washing the conidia by distilled water) and a living sporulation method (i.e., culturing the conidia of pathogenic bacteria of gummy stem blight of melon by using a cucumber living body). In order to obtain a large amount of conidia of pathogenic bacteria of gummy stem blight, dilute acid or lactic acid is added into a liquid culture medium for culturing the conidia of the pathogenic bacteria of gummy stem blight for regulation, so that a large amount of conidia can be generated, but the test operation process is more strict and complex; the colony culture method and the living body sporulation method are difficult to meet the requirements of large-scale disease-resistant back-grafting identification tests or breeding material resistance inoculation identification tests.
Disclosure of Invention
The invention aims to provide a method for improving the spore yield of conidia of pathogenic bacteria of gummy stem blight so as to solve the problems in the prior art.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a method for improving the spore yield of conidia of pathogenic bacteria of gummy stem blight, which comprises the following steps: inoculating pathogenic bacteria of gummy stem blight of melon into a liquid culture medium, and culturing to obtain conidia of the pathogenic bacteria of gummy stem blight of melon; wherein the liquid culture medium contains potassium fulvate.
Further, the content of the potassium fulvate in the liquid culture medium is 0.1g/L.
Further, the formula of the liquid culture medium is specifically as follows: 200g of potato, 10g of glucose, 0.1g of potassium fulvate and 1000mL of distilled water, and the pH value is natural.
Further, the temperature of the culture was 28 ℃.
Further, the rotation speed of the culture is 180r/min.
Further, the potassium fulvate is potassium fulvate.
The invention also provides application of the potassium fulvate in improving the spore production rate or spore production quantity of the conidia of the pathogenic bacteria of the gummy stem blight.
Further, the potassium fulvate is potassium fulvate.
The invention also provides a culture medium for improving the spore yield of conidia of pathogenic bacteria of gummy stem blight of melon, and the culture medium contains potassium fulvate.
Further, the addition amount of the potassium fulvate in the culture medium is 0.1g/L.
The invention discloses the following technical effects:
the invention changes the content of glucose in PD (potato glucose liquid culture medium, culture medium formula potato 200g, glucose 20g, distilled water 1000mL) liquid culture medium for culturing pathogenic bacteria of gummy stem blight, and determines the optimal glucose dosage (10 g)) On the basis, potassium fulvate (with the optimal addition amount of 0.10 g) is added into a PD liquid culture medium for propagation of the conidia of the pathogenic bacteria of the gummy stem blight, and the conidia production amount of the pathogenic bacteria of the gummy stem blight is observed under different temperature conditions. Test results show that the conidiophore of pathogenic bacteria of gummy stem blight induced at 28 ℃ has large conidiophore, and the conidiophore quantity reaches 8.8 multiplied by 10 on the 12 th day of culture 11 one/mL, and good stability. The method simplifies the generation process of conidia of pathogenic bacteria of gummy stem blight under the condition of ensuring the generation of the conidia by inducing the pathogenic bacteria of gummy stem blight, cancels the link of ultraviolet intermittent irradiation and cancels the link of utilizing diluted acid or lactic acid to regulate PD culture medium. According to the invention, potassium fulvate is added into the PD culture medium, so that the conidiospore production rate of pathogenic bacteria of gummy stem blight cultured by the PD liquid culture medium is improved, the culture time of 2 days is shortened, and the conidiospore propagation quantity of gummy stem blight is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a graph showing the observation results of CK treatment at 40 times microscope in Table 1 of example 1;
FIG. 2 is an observation of T1 treatment at 40 times microscope in Table 1 of example 1;
FIG. 3 is an observation of T2 treatment at 40 times microscope in Table 1 of example 1;
FIG. 4 is an observation of the T3 treatment at 40 times microscope in Table 1 of example 1;
FIG. 5 is an observation of T4 treatment at 40 times microscope in Table 1 of example 1;
FIG. 6 is an observation of CK treatment at 40 times microscope in Table 2 of example 1;
FIG. 7 is an observation of T1 treatment at 40 times microscope in Table 2 of example 1;
FIG. 8 is the observation of the T2 treatment at 40 times microscope in Table 2 of example 1;
FIG. 9 is an observation of T3 treatment at 40 times microscope in Table 2 of example 1;
FIG. 10 is a graph showing the observation results of CK treatment at 40 times microscope in Table 3 of example 1;
FIG. 11 is an observation of T1 treatment at 40 times microscope in Table 3 of example 1;
FIG. 12 is an observation of T2 treatment at 40 times microscope in Table 3 of example 1;
FIG. 13 is an observation of T3 treatment at 40 times microscope in Table 3 of example 1;
FIG. 14 is an observation of CK treatment at 40 times microscope in Table 4 of example 1;
FIG. 15 is an observation of T1 treatment at 40 times microscope in Table 4 of example 1;
FIG. 16 is an observation of T2 treatment at 40 times microscope in Table 4 of example 1;
FIG. 17 is the observation of the T3 treatment at 40 times microscope in Table 4 of example 1.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every intervening value, to the extent any stated value or intervening value in a stated range, and any other stated or intervening value in a stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
Example 1
1.1 test materials
Test disease strains: the reference document of the separation method of the melon gummy stem blight type A bacterial strain "separation of fruit bodies of melon gummy stem blight bacteria and research of spore production conditions of the type A bacterial strain".
Test medium: PDA (potato glucose agar solid culture medium, the culture medium formula is that potato 200g, glucose 20g, agar 15g, distilled water 1000mL, pH value is natural); PD (potato glucose liquid culture medium, culture medium formula: 200g of potato, 20g of glucose, 1000mL of distilled water, natural pH value).
1.2 spore production induction of conidia by different factors
1.2.1 sporulation Induction of conidia by Medium
Test 1: under the conditions of the same temperature and the same revolution for culturing the conidia of the pathogenic bacteria of the gummy stem blight, the glucose content in a PD liquid culture medium for culturing the conidia of the pathogenic bacteria of the gummy stem blight is changed, and 0.1g of potassium fulvate is added in the T4 treatment. See table 1 for details. The test bacterial cake was inoculated into each of the 5 treatment groups shown in Table 1 and cultured for 12 days.
TABLE 1 sporulation Induction of conidia by the Medium
1.2.2 spore production Induction of conidia by exogenous factors
Test 2: a total of 4 treatments were set up for this experiment. And (3) adding pathogenic bacteria of gummy stem blight of melon into a PD liquid culture medium, and changing the content of glucose in the PD liquid culture medium to perform a spore production induction experiment. (4 treatment temperature: 25 ℃, revolution: 180r/min. The same amount of mineral source potassium fulvate was added). See table 2 for details. The test bacterial cake was inoculated into each of the 4 treatment groups shown in Table 2 and cultured for 12 days.
TABLE 2 spore production induction of geminized spores by exogenous factors
1.2.3 temperature and exogenous factors inducing conidia to produce spores
Test 3: a total of 4 treatments were set up for this experiment. Adding potassium fulvate with different contents into a liquid culture medium, setting different temperature gradients (22 ℃ and 28 ℃), and observing the conidium amount of pathogenic bacteria of gummy stem blight. See tables 3 and 4 for details. The test bacterial cake was inoculated into each of the 4 treatment groups shown in Table 3 and 4 treatment groups shown in Table 4, and cultured for 12 days.
TABLE 3 temperature and spore production Induction of conidia by exogenous factors
TABLE 4 sporulation Induction of conidia by temperature and exogenous factors
2 results and analysis
2.1 sporulation Induction of conidia by Medium
In the experiment of culturing conidia of pathogenic bacteria of gummy stem blight, the glucose content in the conventional PD liquid medium formula was changed under the same conditions of culture temperature and shaking table rotation number. As can be seen from FIGS. 1, 2, 3, 4 and 5, compared with CK treatment (conventional PD broth formulation: potato 200g, glucose 20g, distilled water 1000mL, pH value natural), T1 (glucose 5 g) and T3 (glucose 15 g) did not induce conidia of pathogenic bacteria of gummy stem blight, and T2 (glucose 15 g) induced conidia of pathogenic bacteria of gummy stem blight with a small amount of conidia, about 1X 10 3 spores/mL (see Table 5). The T4 treatment (20 g of glucose and 0.10g of potassium fulvate) can induce the generation of conidia of pathogenic bacteria of gummy stem blight of melon, and the conidia amount reaches 8.9 multiplied by 10 at the 12 th day of culture 11 spores/mL.
TABLE 5 Effect of different treatments on conidia count of pathogenic bacteria of gummy stem blight of melon
2.2 spore production Induction of conidia by Medium
As can be seen from FIGS. 6, 7, 8 and 9, compared with CK treatment (conventional PD broth formulation: potato 200g, glucose 20g, distilled water 1000mL, pH value natural), T1 (glucose 5g + potassium fulvate 0.10 g) did not induce conidium production of pathogenic bacteria of gummy stem blight of melon; the generation of conidia of pathogenic bacteria of gummy stem blight can be induced under the condition of T2 treatment (10 g of glucose and 0.10g of potassium fulvate), and the conidia amount reaches 8.8 multiplied by 10 at the 12 th day of culture 9 spores/mL (see table 6); t3 treatment (glucose 15g + potassium fulvate 0.10 g) failed to induce pathogenic bacteria of gummy stem blight at day 12 of cultureConidiophores but induces conidiophores.
TABLE 6 Effect of different treatments on conidia count of pathogenic bacteria of gummy stem blight
2.3 temperature and exogenous factors induced spore production of conidia
As can be seen from FIGS. 10, 11, 12 and 13, the generation of conidia of pathogenic bacteria of gummy stem blight of melon could not be induced under the condition of T1 (10 g of glucose + 0.05g of potassium fulvate) compared with CK treatment (PD liquid medium formula: potato 200g, 10g of glucose, 1000mL of distilled water, natural pH value) at 22 ℃; the T2 treatment (10 g of glucose and 0.10g of potassium fulvate) can induce the generation of conidia of pathogenic bacteria of gummy stem blight of melon, and the conidia amount reaches 11.4 multiplied by 10 at the 12 th day of culture 10 spores/mL (see table 7); the T3 treatment (10 g of glucose and 0.20g of potassium fulvate) can induce the generation of conidia of pathogenic bacteria of gummy stem blight at the 12 th day of culture, and the conidia amount reaches 10.0 multiplied by 10 10 spores/mL.
TABLE 7 Effect of different treatments on conidia count of pathogenic bacteria of gummy stem blight of melon
As can be seen from FIGS. 14, 15, 16 and 17, the production of conidia of pathogenic bacteria of gummy stem blight of melon was induced under T1 (10 g glucose + 0.05g potassium fulvate) at 28 ℃ as compared with CK treatment (PD broth formulation: potato 200g, 10g glucose, 1000mL distilled water, natural pH) and the conidia amount reached 8.4X 10 at 12 days of culture 3 spores/mL (see table 8); the generation of conidia of pathogenic bacteria of gummy stem blight can be induced under the condition of T2 treatment (10 g of glucose and 0.10g of potassium fulvate), and the conidia amount reaches 8.8 multiplied by 10 at the 12 th day of culture 11 spore/mL; t3 treatment (glucose 10g + Potassium Fulvate)0.20 g) can induce the generation of conidia of pathogenic bacteria of gummy stem blight at the 12 th day of culture, and the conidia amount reaches 1.4 multiplied by 10 4 spores/mL.
TABLE 8 Effect of different treatments on conidia count of pathogenic bacteria of gummy stem blight of melon
In conclusion, on the basis of the conventional PD liquid culture medium formula (200 g of potato, 10g of glucose, 1000mL of distilled water and natural pH value), the regulation of the glucose content alone cannot induce the generation of conidia of pathogenic bacteria of the gummy stem blight of the melon. But the generation of conidia of pathogenic bacteria of gummy stem blight can be induced by adjusting the glucose content and adding a certain amount of potassium fulvate, and the conidia amount is large at the 12 th day of culture. Simultaneously changing the temperature and the glucose content, and adding different contents of potassium fulvate on the basis, wherein at the temperature of 22 ℃, compared with CK, conidia of pathogenic bacteria of gummy stem blight can not be induced under the condition of T1 (10 g of glucose and 0.05g of potassium fulvate); however, conidia induced by T2 (glucose 10g + potassium fulvate 0.10 g) and T3 (glucose 10g + potassium fulvate 0.20 g) treatments are large, conidia induced by T2 (glucose 10g + potassium fulvate 0.10 g) treatments are large, and the stability is good. Compared with CK at the temperature of 28 ℃, T1 (10 g of glucose and 0.05g of potassium fulvate), T2 (10 g of glucose and 0.10g of potassium fulvate) and T3 (10 g of glucose and 0.20g of potassium fulvate) can induce the generation of conidia of pathogenic bacteria of gummy stem blight, and the conidia amount induced by the culture at the temperature of 28 ℃ to the 12 th day is more than that induced at the temperatures of 22 ℃ and 25 ℃.
Comparative example 1
Inoculating Rhizoctonia solani (potato 200g, glucose 20g, NH) with PD culture medium (potato glucose liquid culture medium) 4 H 2 PO 4 2g, 1000mL of distilled water), placing in an incubator at 25 ℃, performing dark culture for 7d, performing 4d (12 h ultraviolet lamp/12 h dark) intermittent ultraviolet lamp treatment, then performing culture in the dark, and calculating the score after 14 days of total cultureThe amount of sporogenous spores.
The number of conidia after 12 days of culture in the T2 treatment group in Table 8 of example 1 was counted, and the number of conidia after 14 days of culture in comparative examples 1-2 was counted, and the results are shown in Table 9.
In comparative example 1, after 14 days of culture in PD medium, the conidium amount of pathogenic bacteria of gummy stem blight was measured to be 2X 10 3 The conidium amount of pathogenic bacteria of gummy stem blight cultured by the method of T2 in Table 8 of example 1 is 8.8X 10 days after 12 days of culture 11 Per mL, much higher than in comparative examples 1-2.
TABLE 9 Effect of different treatments on conidia count of pathogenic bacteria of gummy stem blight
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (10)
1. A method for improving the spore yield of conidia of pathogenic bacteria of gummy stem blight is characterized by comprising the following steps: inoculating pathogenic bacteria of gummy stem blight of melon into a liquid culture medium, and culturing to obtain conidia of the pathogenic bacteria of gummy stem blight of melon; wherein the liquid culture medium contains potassium fulvate.
2. The method according to claim 1, wherein the content of the potassium fulvate in the liquid medium is 0.1g/L.
3. The method according to claim 1, wherein the liquid medium is formulated as: 200g of potato, 10g of glucose, 0.1g of potassium fulvate and 1000mL of distilled water, and the pH value is natural.
4. The method according to claim 1, wherein the temperature of the culture is 28 ℃.
5. The method according to claim 1, wherein the rotation speed of the cultivation is 180r/min.
6. The method of claim 1, wherein the potassium fulvate is potassium fulvate.
7. The application of the potassium fulvate in improving the spore production rate or the spore production amount of conidia of pathogenic bacteria of gummy stem blight.
8. Use according to claim 7, wherein the potassium fulvate is potassium fulvate.
9. A culture medium for increasing the spore yield of conidia of pathogenic bacteria of gummy stem blight, which is characterized by comprising potassium fulvate.
10. The culture medium according to claim 9, wherein the potassium fulvate is added to the culture medium in an amount of 0.1g/L.
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| WO2014043485A1 (en) * | 2012-09-13 | 2014-03-20 | Fbsciences Holdings, Inc. | Compositions and method of controlling fungus |
| CN110331098A (en) * | 2019-07-11 | 2019-10-15 | 浙江省农业科学院 | A kind of sporogenic method of promotion blight dis-ease pathogen |
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|---|---|---|---|---|
| CN1122650A (en) * | 1995-07-04 | 1996-05-22 | 河南省科学院化学研究所 | Xanthohumate |
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