CN115322775B - Fingerprint developing agent based on nitrogen-doped carbon quantum dots, preparation method and application - Google Patents
Fingerprint developing agent based on nitrogen-doped carbon quantum dots, preparation method and application Download PDFInfo
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- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 49
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000008280 blood Substances 0.000 claims abstract description 40
- 210000004369 blood Anatomy 0.000 claims abstract description 40
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 38
- 229920000742 Cotton Polymers 0.000 claims abstract description 33
- 230000005284 excitation Effects 0.000 claims abstract description 22
- 239000004519 grease Substances 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 18
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- FVDOBFPYBSDRKH-UHFFFAOYSA-N perylene-3,4,9,10-tetracarboxylic acid Chemical compound C=12C3=CC=C(C(O)=O)C2=C(C(O)=O)C=CC=1C1=CC=C(C(O)=O)C2=C1C3=CC=C2C(=O)O FVDOBFPYBSDRKH-UHFFFAOYSA-N 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims abstract description 7
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 5
- 238000012800 visualization Methods 0.000 claims abstract description 5
- 239000004745 nonwoven fabric Substances 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 3
- 230000003287 optical effect Effects 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 7
- 229910021641 deionized water Inorganic materials 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 238000003825 pressing Methods 0.000 claims description 6
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 238000001179 sorption measurement Methods 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 12
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000011840 criminal investigation Methods 0.000 abstract description 4
- 125000003277 amino group Chemical group 0.000 abstract description 3
- 239000002904 solvent Substances 0.000 abstract description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 11
- 229910052782 aluminium Inorganic materials 0.000 description 11
- 239000011888 foil Substances 0.000 description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 238000003917 TEM image Methods 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- WSRHMJYUEZHUCM-UHFFFAOYSA-N perylene-1,2,3,4-tetracarboxylic acid Chemical compound C=12C3=CC=CC2=CC=CC=1C1=C(C(O)=O)C(C(O)=O)=C(C(O)=O)C2=C1C3=CC=C2C(=O)O WSRHMJYUEZHUCM-UHFFFAOYSA-N 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- IAZDPXIOMUYVGZ-WFGJKAKNSA-N Dimethyl sulfoxide Chemical compound [2H]C([2H])([2H])S(=O)C([2H])([2H])[2H] IAZDPXIOMUYVGZ-WFGJKAKNSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
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- 238000002189 fluorescence spectrum Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000002329 infrared spectrum Methods 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012984 biological imaging Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- FPVGTPBMTFTMRT-UHFFFAOYSA-L disodium;2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=CC=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 FPVGTPBMTFTMRT-UHFFFAOYSA-L 0.000 description 1
- 235000019233 fast yellow AB Nutrition 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000003958 fumigation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- CLYVDMAATCIVBF-UHFFFAOYSA-N pigment red 224 Chemical compound C=12C3=CC=C(C(OC4=O)=O)C2=C4C=CC=1C1=CC=C2C(=O)OC(=O)C4=CC=C3C1=C42 CLYVDMAATCIVBF-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/0883—Arsenides; Nitrides; Phosphides
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Abstract
The invention relates to a fingerprint revealing agent based on nitrogen doped carbon quantum dots, a preparation method and application thereof. Dissolving perylene tetracarboxylic acid in dimethyl sulfoxide solution, adding ethylenediamine, fully mixing, reacting under microwave conditions to obtain nitrogen-doped carbon quantum dots with carboxyl and amino groups on the surfaces, and preparing a fingerprint developing agent by taking water as a solvent; the non-woven fabric is used as a base material, the latent fingerprint developing agent is loaded, the latent fingerprint developing cotton sheet is prepared, and the grease latent fingerprint and the latent blood fingerprint are developed by adopting a cotton sheet developing method. The latent fingerprint revealing cotton piece provided by the invention can reveal oil latent fingerprints and latent blood fingerprints, so that fingerprint revealing is more efficient; and the luminous color of the fingerprint pattern after appearance can be adjusted by changing the wavelength of the excitation light, so that the interference of the object background fluorescence can be effectively avoided. The visualization technology provided by the invention is simple and convenient to operate and low in cost, and can be suitable for criminal investigation sites to cope with various complex conditions.
Description
Technical Field
The invention relates to a fingerprint development technology, in particular to a fingerprint development agent based on a carbon quantum dot solution and a method for developing grease latent fingerprints and latent blood fingerprints on different objects, belonging to the field of carbon nano fluorescent material application.
Background
The fingerprint becomes an important basis for identity recognition due to the characteristics of different people, unchanged life and trace of touching objects, and plays an important role in criminal investigation, inspection and authentication. With the continuous improvement of anti-reconnaissance consciousness of criminals, visible fingerprints with high definition are not common at crime sites, while latent fingerprints are often ignored by criminals because the latent fingerprints are invisible to naked eyes, so the fingerprint revealing technology is the core and key of the fingerprint technology.
Fingerprints are common fingerprints at crime sites including grease fingerprints and blood fingerprints according to differences in composition. The traditional method for displaying the grease fingerprint comprises a powder method, a physical visualization agent method, a chemical fumigation method and the like; the common occult blood fingerprint developing agent in China comprises tetramethyl benzidine, amino black 10B, acid yellow and the like. However, the conventional revealing technology cannot meet the first-line criminal investigation requirement due to certain defects, and particularly, most of the conventional revealing methods can only reveal one fingerprint, and the components of the fingerprint need to be judged before revealing, so that inconvenience is brought to criminal investigation. Therefore, the development of a simple, efficient and universal latent fingerprint display method has important significance.
Fluorescent agents are focused by fingerprint imaging workers because of their characteristic luminescence properties that are resistant to interference from fluorescence of the guest background. In recent years, the carbon quantum dot has very wide application in the fields of fluorescence sensing, biological imaging, biomedicine and the like due to the characteristics of excellent optical performance, low toxicity, good biocompatibility, simple preparation and the like. And most of the carbon quantum dots have the dependency of excitation light wavelength, so that the carbon quantum dots have great application prospect in multicolor imaging. At present, carbon quantum dots have been reported to be used in the field of latent fingerprint development, for example: carbon quantum dots of a single luminescent color were prepared for developing latent fingerprints of grease on different objects (see ACS appl. Mater. Interfaces 2020, 12, 29549-29555 and J. Colloid Interface Sci. 2019, 554, 344-352). The literature also reports that a carbon quantum dot with the dependence of excitation light wavelength is used for displaying grease latent fingerprints on different objects (see literature: animal Methods 2017, 9, 4770-4775), but the development of latent blood fingerprints is not involved.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention provides a fingerprint revealing agent based on nitrogen doped carbon quantum dots, a preparation method and a method for revealing latent fingerprints, wherein the fingerprint revealing agent can be used for revealing the latent fingerprints and the latent blood fingerprints, and the fluorescent interference of object background can be prevented by adjusting the wavelength of excitation light to obtain fingerprint images with various colors.
The technical scheme for realizing the invention is to provide a preparation method of a fingerprint revealing agent based on nitrogen doped carbon quantum dots, which comprises the following steps:
(1) According to the parts by weight of substances, 1 part of perylene tetracarboxylic acid is dissolved in dimethyl sulfoxide solution, 2.5-25 parts of ethylenediamine is added, after full mixing, the mixture is reacted for 5-8 minutes under the condition that the microwave power is 700-900W, and the mixture is purified to obtain the nitrogen-doped carbon quantum dot with carboxyl and amino on the surface;
(2) And (3) dissolving the nitrogen-doped carbon quantum dots obtained in the step (1) into deionized water according to the concentration of 5X 10 -5 g/L~1×10-4 g/L to obtain the fingerprint developing agent based on the nitrogen-doped carbon quantum dots.
The technical scheme of the invention comprises the fingerprint revealing agent based on the nitrogen doped carbon quantum dots, which is obtained by the preparation method.
According to the fingerprint revealing agent, different excitation wavelengths are selected within the range of 300-520 nm, and multicolor fluorescence emission with different wavelengths and maximum emission wavelength within the range of 436-545 nm is obtained.
The technical scheme of the invention also comprises application of the fingerprint developing agent based on the nitrogen-doped carbon quantum dots, and the fingerprint developing agent is prepared into a latent fingerprint developing cotton sheet for developing grease latent fingerprints and latent blood fingerprints.
The latent fingerprint developing cotton piece is prepared by immersing a non-woven fabric serving as a base material in a fingerprint developing agent, wherein the amount of the adsorption developing agent is 5 mu L/cm 2～50 μL/cm2.
The invention relates to a fingerprint revealing agent based on nitrogen doped carbon quantum dots, which is applied to revealing of occult blood fingerprints and comprises the following steps:
(1) Fixation of fresh occult blood fingerprint blood: immersing the object loaded with the fresh occult blood fingerprint in absolute ethyl alcohol, immersing for 2-3 min, taking out and naturally airing; or standing the object loaded with fresh occult blood fingerprint in a shade and dry place for more than 12 hours;
(2) Flatly covering the latent fingerprint appearance cotton sheet on the object carrying the latent blood fingerprint processed in the step (1), slightly pressing to enable the cotton sheet to be fully contacted with the object, and standing for 1-5 min;
(3) And (3) stably removing the cotton piece from the object, drying, adopting an excitation wavelength in a range of 300-520 nm, selecting an optical filter matched with the emission wavelength, and shooting to obtain the visualized occult blood fingerprint image.
The invention relates to a fingerprint revealing agent based on nitrogen doped carbon quantum dots, which is applied to revealing grease latent fingerprints, and comprises the following steps:
(1) Flatly covering the latent fingerprints with cotton sheets on the object carrying the grease latent fingerprints, slightly pressing to enable the cotton sheets to fully contact with the object, standing for 1-5 min, and taking out the object;
(3) And (3) stably removing the cotton piece from the object, drying, adopting an excitation wavelength in a range of 300-520 nm, selecting an optical filter matched with the emission wavelength, and shooting to obtain the visualized occult blood fingerprint image.
According to the invention, perylene tetracarboxylic acid is used as a carbon source, and the carbon quantum dot solution doped with ethylenediamine is used as a developing agent, and as the surface of the carbon quantum dot contains amino groups, the amino groups can act with proteins in blood, so that the occult blood fingerprint can be developed; and because of the large conjugated structure of perylene tetracarboxylic, the maximum emission wavelength range of the carbon quantum dots is wide, so that the interference of object background fluorescence can be effectively resisted, and a fingerprint image with clear lines and high ridge-groove contrast is obtained.
Compared with the prior art, the invention has the beneficial effects that:
1. the surface of the carbon quantum dot prepared by the invention has a large number of amino and carboxyl groups, and can be used as reaction sites to act with proteins in blood, so that the latent blood fingerprint can be displayed while the grease fingerprint is displayed.
2. After the latent fingerprint is developed by the latent fingerprint development method provided by the invention, fingerprint images (blue-red) with different luminous colors can be obtained by changing the wavelength of the excitation light and matching with the optical filters with corresponding emission wavelengths, so that the fluorescent interference of object background is effectively avoided, and the development effect is clear and bright.
3. The latent fingerprint developing method provided by the invention is simple to operate, the developing cotton sheets can be stored for a long time, the concentration of the contained latent fingerprint developing agent is low, the using amount is small, and the using cost is low.
4. The latent fingerprint developing agent provided by the invention uses water as a solvent, has low toxicity and can be stored for a long time.
Drawings
FIG. 1 is a nuclear magnetic spectrum of perylene tetracarboxylic acids prepared in accordance with examples of the present invention;
FIG. 2 is an infrared spectrum of a carbon quantum dot prepared according to an embodiment of the present invention;
FIG. 3 is a transmission electron micrograph of carbon quantum dots prepared according to an embodiment of the present invention;
FIG. 4 is a graph showing fluorescence emission spectra of carbon quantum dot solutions prepared according to embodiments of the present invention at different excitation light wavelengths;
Fig. 5 is a photograph of a occult blood fingerprint not developed on an aluminum foil object provided in an embodiment of the present invention:
FIG. 6 is a photograph of occult blood fingerprint after appearance on an aluminum foil object provided by an embodiment of the present invention under different excitation light conditions;
Fig. 7 is a photograph of a latent oil fingerprint not developed on an aluminum foil object provided in an embodiment of the present invention;
Fig. 8 is a photograph of a latent oil fingerprint on an aluminum foil object according to an embodiment of the present invention under 365 nm excitation lights.
Detailed Description
The technical scheme of the invention is further described below with reference to the accompanying drawings and examples.
Example 1
The embodiment provides a carbon quantum dot solution fingerprint developing cotton piece with perylene tetracarboxylic acid as a carbon source, and the preparation method comprises the following steps:
1. Preparation of perylene tetracarboxylic acids:
3,4,9, 10-perylene tetracarboxylic dianhydride (5.00 g, 12.74 mmol), potassium hydroxide (5.00 g, 89.13 mmol), 100mL deionized water and reflux stirring 12h were added into the flask in sequence. Cooling to room temperature, adding 100mL of 10% sulfuric acid, stirring thoroughly, filtering to obtain red solid, and drying. Yield 5.12 g (94%). 1H NMR (400 MHz, DMSO-d6, delta) 8.01 (s, 4H), 8.61 (t, 4H), 13.04 (s, 4H).
Referring to fig. 1, the nuclear magnetic spectrum of perylene tetracarboxylic acid provided in this example is shown.
2. Preparing carbon quantum dots by taking perylene tetracarboxylic acid as a carbon source:
Adding perylene tetracarboxylic acid (0.251 g,0.59 mmol), 15 and mL dimethyl sulfoxide into a pre-dried 400 and mL beaker, stirring until the perylene tetracarboxylic acid and the dimethyl sulfoxide are fully dissolved, performing ultrasonic treatment on the mixture for 1 min, adding ethylenediamine (900 mu L,12.80 and mmol), stirring the mixture at normal temperature until the ethylenediamine and the ethylenediamine are fully mixed, covering the beaker with a preservative film, and placing the preservative film in a microwave oven for microwave reaction for 5 and min. After the reaction is finished, cooling to room temperature, adding 15 mL deionized water to enable reactants to be fully dispersed in the deionized water, centrifuging, dispersing solids obtained by centrifuging in 15 mL deionized water, filtering by a filter head with the diameter of 0.45 mu m, and finally freeze-drying filtrate to obtain deep purple solids, namely the carbon quantum dots.
Referring to fig. 2, an infrared spectrum of the carbon quantum dot provided in this embodiment is shown, where the surface of the carbon quantum dot contains carboxyl and amino.
Referring to fig. 3, it is a transmission electron micrograph of the carbon quantum dot in the embodiment, a graph a is a transmission electron micrograph of the carbon quantum dot amplified by 3.5K times, and b graph b is a transmission electron micrograph of the carbon quantum dot amplified by 150K times, and it can be seen that the particle size of the particles is about 50nm, and the particles are in a more regular sphere shape.
Referring to fig. 4, the fluorescence emission spectra of the carbon quantum dot solution in this embodiment under different excitation light wavelengths show that the maximum emission wavelength of the carbon quantum dot solution can be adjusted to 436-545 nm by adjusting the excitation light wavelength in the range of 300-520 nm.
3. Preparing a latent fingerprint developing agent:
And taking 0.5 g carbon quantum dot solid in a 10mL centrifuge tube, adding 5 mL deionized water, and shaking the centrifuge tube to uniformly disperse the carbon quantum dot to obtain the 5 mL latent fingerprint developing agent.
4. Preparing a developing cotton piece loaded with a developing agent:
400 mu L of visualization reagent is taken in a weighing bottle; after two cotton non-woven fabric substrates are cut according to the size of 2 cm multiplied by 3 cm, the substrates are placed into a weighing bottle for soaking 1 min, and the latent fingerprint developing cotton sheet with the load developing agent of about 20 mu L/cm 2 is obtained.
Sealing the weighing bottle filled with the cotton sheets with latent fingerprints, and storing in a shade and dry place.
Example 2
The embodiment provides a method for displaying the occult blood fingerprint by using the display cotton sheet prepared in the embodiment 1 aiming at the fresh occult blood fingerprint on the surface of an aluminum foil object, which comprises the following specific steps:
1. standing the object loaded with the fresh occult blood fingerprint in a shade and dry place for 12 hours;
2. the latent blood fingerprint developing cotton piece loaded with the latent blood fingerprint developing agent in the embodiment 1 is covered on the object loaded with the latent blood fingerprint in a flat way, the cotton piece is fully contacted with the object by slightly pressing, and the object is kept stand for 2 min;
3. And (3) stably removing the cotton piece from the object, drying, and then selecting an optical filter matched with the emission wavelength under the illumination condition of 300-520 nm wavelength, and shooting to obtain the developed occult blood fingerprint images with different colors.
Referring to fig. 5, a photograph of a occult blood fingerprint not appearing on an aluminum foil object provided in this embodiment is shown in a drawing a under natural light, and in a drawing b under 365nm light; as can be seen from the figure, the fingerprint pattern is not observed under 365nm light excitation, and the fingerprint ridge-groove contrast is low under natural light.
Referring to fig. 6, a photograph of a occult blood fingerprint after being developed on an aluminum foil object provided in this embodiment under different excitation light conditions is shown: wherein, a is a fingerprint photo excited at 405 nm and matched with 445 nm optical filters, and the fingerprint ridge image is yellow-green; b is a fingerprint photo excited at 445 nm and matched with 495 nm filter, the fingerprint ridge image is yellow; c, under 470 nm excitation, matching 535 nm fingerprint photo of the filter, wherein the fingerprint ridge image is orange yellow; d is a fingerprint photograph excited at 500 nm and matched with a 570 nm filter, and the fingerprint ridge image is red. The latent fingerprint display method has good latent blood fingerprint effect, clear fingerprint pattern and changeable excitation light wavelength as required, so as to change the color of the fingerprint.
Example 3
The embodiment provides a method for displaying grease latent fingerprints on the surface of an aluminum foil object by using the cotton piece prepared in the embodiment 1, which comprises the following specific steps:
1. the cotton sheets with latent fingerprints prepared in the example 1 are flatly covered on an aluminum foil object loaded with grease latent fingerprints, the cotton sheets are fully contacted with the object by slightly pressing, and the object is taken out after standing for 2 min;
2. And (3) stably removing the cotton piece from the object, drying, and shooting under the illumination condition with the wavelength of 365 nm to obtain the grease latent fingerprint image.
Referring to fig. 7, it is a photograph of a latent fingerprint of grease which is not developed on the aluminum foil object provided in this example 3, a is a photograph under natural light, b is a photograph under 365 nm light, and it can be seen from the figure that the latent fingerprint pattern which is not developed is not observed under 365 nm excitation light, and the fingerprint ridge-groove contrast is low under natural light.
Referring to fig. 8, a photograph of the latent grease fingerprint developed on the aluminum foil object provided in this example under 365 nm excitation light conditions; as can be seen from fig. 8, the method for developing latent fingerprints in the present invention has good effects on grease latent fingerprints, and the developed fingerprint patterns are clear.
Claims (7)
1. The preparation method of the fingerprint developing agent based on the nitrogen-doped carbon quantum dots is characterized by comprising the following steps:
(1) According to the parts by weight of the substances, 1 part of perylene tetracarboxylic acid is dissolved in dimethyl sulfoxide solution, 2.5-25 parts of ethylenediamine is added, after full mixing, the mixture is reacted for 5-8 minutes under the condition that the microwave power is 700-900W, and the mixture is purified to obtain the nitrogen-doped carbon quantum dot with carboxyl and amino on the surface;
(2) And (3) dissolving the nitrogen-doped carbon quantum dots obtained in the step (1) into deionized water according to the concentration of 5X 10 -5 g/L~1×10-4 g/L to obtain the fingerprint developing agent based on the nitrogen-doped carbon quantum dots.
2. A fingerprint developing agent based on nitrogen-doped carbon quantum dots obtained by the preparation method of claim 1.
3. The fingerprint developing agent based on nitrogen-doped carbon quantum dots according to claim 2, wherein: different excitation wavelengths are selected within the range of 300-520 nm, and multicolor fluorescence emission with different wavelengths and maximum emission wavelength within the range of 436-545 nm is obtained.
4. The use of a fingerprint developing agent based on nitrogen-doped carbon quantum dots as claimed in claim 2, characterized in that: the fingerprint developing agent is prepared into a latent fingerprint developing cotton sheet for developing grease latent fingerprints and latent blood fingerprints.
5. The use of a fingerprint developing agent based on nitrogen-doped carbon quantum dots according to claim 4, wherein: the non-woven fabric is used as a base material, the base material is immersed in the fingerprint developing agent, the amount of the adsorption developing agent is 5 mu L/cm 2～50 μL/cm2, and the latent fingerprint developing cotton sheet is prepared.
6. The use of a fingerprint developing agent based on nitrogen-doped carbon quantum dots according to claim 4 or 5, characterized in that: the method is applied to the visualization of the occult blood fingerprint and comprises the following steps:
(1) Fixation of fresh occult blood fingerprint blood: immersing the object loaded with the fresh occult blood fingerprint in absolute ethyl alcohol, immersing for 2-3 min, taking out and naturally airing; or standing the object loaded with fresh occult blood fingerprint in a shade and dry place for more than 12 hours;
(2) Flatly covering the latent fingerprint appearance cotton sheet on the object carrying the latent blood fingerprint processed in the step (1), slightly pressing to enable the cotton sheet to be fully contacted with the object, and standing for 1-5 min;
(3) And (3) stably removing the cotton piece from the object, drying, adopting an excitation wavelength in a range of 300-520 nm, selecting an optical filter matched with the emission wavelength, and shooting to obtain the visualized occult blood fingerprint image.
7. The use of a fingerprint developing agent based on nitrogen-doped carbon quantum dots according to claim 4 or 5, characterized in that: the method is applied to the visualization of the grease latent fingerprints and comprises the following steps:
(1) Flatly covering the latent fingerprints with cotton sheets on the object carrying the grease latent fingerprints, slightly pressing to enable the cotton sheets to fully contact with the object, standing for 1-5 min, and taking out the object;
(3) And (3) stably removing the cotton piece from the object, drying, adopting an excitation wavelength in a range of 300-520 nm, selecting an optical filter matched with the emission wavelength, and shooting to obtain the developed grease latent fingerprint image.
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