CN115321064A - Low-temperature storage system for biological samples - Google Patents
Low-temperature storage system for biological samples Download PDFInfo
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- CN115321064A CN115321064A CN202210776347.XA CN202210776347A CN115321064A CN 115321064 A CN115321064 A CN 115321064A CN 202210776347 A CN202210776347 A CN 202210776347A CN 115321064 A CN115321064 A CN 115321064A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G1/00—Storing articles, individually or in orderly arrangement, in warehouses or magazines
- B65G1/02—Storage devices
- B65G1/04—Storage devices mechanical
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Abstract
The invention discloses a cryogenic storage system for biological samples, comprising: the device comprises a vacuum liquid nitrogen tank, a carrying disc for placing a test tube sample, a freezing frame outlet, a carrying disc discharge port, a first cover opening mechanism, a second cover opening mechanism, a freezing frame storage frame rotating mechanism and a lifting discharge mechanism, wherein the freezing frame outlet is used for the freezing frame to pass in and out, the carrying disc discharge port is used for the carrying disc to pass in and out, the first cover opening mechanism is used for opening or sealing the freezing frame outlet, the second cover opening mechanism is used for opening or sealing the carrying disc discharge port, and the lifting discharge mechanism is used for driving the freezing frame to move up and down. Through the mode, the low-temperature storage system for the biological sample can realize full-automatic low-temperature access operation of the biological sample, effectively improve the working efficiency and the production capacity, effectively ensure the activity of the sample and improve the safety of the stored sample.
Description
Technical Field
The invention relates to the technical field of low-temperature storage, in particular to a low-temperature storage system for biological samples.
Background
When storing biological samples, it is necessary to provide a low temperature environment in which the samples can be kept active, by means of liquid nitrogen or the like.
At present, operations such as loading and unloading, transportation and the like of samples are generally carried out in a manual mode, and although some automatic adjustment is carried out to realize a simple storage function, the prior art still has many problems, such as low utilization rate of the internal space of a liquid nitrogen tank, small storage amount of samples in unit equipment, high consumption of liquid nitrogen, easy failure in the automatic process and the like.
Disclosure of Invention
The invention mainly solves the technical problem of providing the low-temperature storage system for the biological sample, has the advantages of high reliability, accurate positioning, compact structure and the like, and has wide market prospect in the application and popularization of the low-temperature sample storage system.
In order to solve the technical problems, the invention adopts a technical scheme that:
a cryogenic storage system for biological samples is provided, comprising: a vacuum liquid nitrogen tank, a freezing frame for placing a carrying disc, a freezing frame outlet for the freezing frame to pass in and out, a carrying disc discharge port for the carrying disc to pass in and out, a first cover opening mechanism for opening or sealing the freezing frame outlet, a second cover opening mechanism for opening or sealing the carrying disc discharge port, a freezing frame storage frame rotating mechanism and a lifting discharge mechanism for driving the freezing frame to move up and down,
the freezing frame outlet is arranged on the upper part of the vacuum liquid nitrogen tank, the carrier plate discharge port is arranged on the side wall of the freezing frame outlet, the freezing frame storage frame rotating mechanism suspends the freezing frame in the vacuum liquid nitrogen tank, and the freezing frame is driven to rotate in the vacuum liquid nitrogen tank to the position below the freezing frame outlet so as to be stored and taken.
In a preferred embodiment of the invention, the lifting heat preservation bin cover is arranged on the discharge port, the opening and closing mechanism and the lifting discharge mechanism to form an outer low-temperature area for protecting the sample.
In a preferred embodiment of the present invention, the rotating mechanism of the storage frame of the cryopreservation rack comprises a rotation driving mechanism, a cantilever shaft, a landing frame, a partition plate, a hollow connecting groove, and a central liquid storage tank for supplying liquid nitrogen, wherein the lower part of the cantilever shaft is connected with the top of the landing frame to suspend the landing frame in the vacuum liquid nitrogen tank, the upper end of the cantilever shaft is connected with the rotation driving mechanism to drive the landing frame to rotate in the vacuum liquid nitrogen tank, the partition plate array is arranged on the landing frame to form a plurality of cryopreservation spaces for placing the cryopreservation rack, the hollow connecting groove is arranged in the landing frame, and the central liquid storage tank is arranged in the hollow connecting groove and conveys the liquid nitrogen in the central liquid storage tank to the bottom of the vacuum liquid nitrogen tank and/or the hollow cooling interlayer on the side of the vacuum liquid nitrogen tank to increase the storage capacity of the liquid nitrogen.
In a preferred embodiment of the present invention, the landing frame includes a central axis of the storage tray and a supporting tray surrounding the bottom of the central axis of the storage tray, the hollow connecting slots are disposed in the central axis of the storage tray, the openings of the hollow connecting slots face downward, and the partition plates are disposed on the supporting tray in an array around the central axis of the storage tray to form a plurality of freezing spaces for placing the fan-shaped structure of the freezing rack.
In a preferred embodiment of the present invention, the first cap opening mechanism includes an opening cap movably connected to the outlet of the cryopreservation rack, and a first driving mechanism for driving the opening cap to lift and rotate.
In a preferred embodiment of the present invention, the second lid opening mechanism includes a sealing lid and a second driving mechanism for synchronously driving the sealing lid to lift and move back and forth, so that the sealing lid opens or seals the discharge hole of the carrying tray.
In a preferred embodiment of the present invention, the second driving mechanism includes a second driving fixing frame, a lifting transmission guide slot, a telescopic transmission guide slot, a transmission guide wheel, a transmission link rod, and a power device, the lifting transmission guide slot and the telescopic transmission guide slot are disposed on the second driving fixing frame, an end of the transmission link rod is connected to the sealing cover, and the transmission link rod is movably connected to the lifting transmission guide slot and the telescopic transmission guide slot respectively through the transmission guide wheel to lift and telescopically guide the transmission link rod and the sealing cover, the power device drives the transmission link rod to move along the guide slot, so that the sealing cover descends and inwardly approaches to the sealed loading tray discharge port, or ascends and outwardly leaves away from the opened loading tray discharge port, wherein the lifting transmission guide slot adopts a linear structure disposed along a vertical direction, and the telescopic transmission guide slot adopts an arc-shaped or L-shaped structure with a lower end extending toward a direction approaching to the loading tray discharge port.
In a preferred embodiment of the invention, the transmission link comprises a lifting transmission part and a telescopic transmission part, one end of the transmission link is connected with the power device, the other end of the transmission link is connected with the lifting transmission part, one end of the telescopic transmission part is rotatably connected with the lifting transmission part, the other end of the telescopic transmission part is rotatably connected with the sealing cover, the lifting transmission part or the telescopic transmission part is slidably connected with the lifting transmission guide groove through a transmission guide wheel so as to perform lifting guidance, so that the sealing cover moves up and down, and the end part of the telescopic transmission part connected with the sealing cover is slidably connected with the telescopic transmission guide groove through the transmission guide wheel so as to perform telescopic guidance, so that the sealing cover is close to or far away from the discharge hole of the carrier disc.
In a preferred embodiment of the present invention, the lifting and discharging mechanism includes a freezing rack gripper, a gripper fixing frame, a first-stage lifting module, and a second-stage lifting module, the freezing rack gripper for gripping the freezing rack is disposed on the gripper fixing frame, the first-stage lifting module is connected to the second-stage lifting module and drives the second-stage lifting module to move up and down, the second-stage lifting module drives the freezing rack gripper to move up and down through the gripper fixing frame, and a lifting power mechanism is connected to the first-stage lifting module and the second-stage lifting module to perform synchronous driving of the multi-stage lifting modules.
In a preferred embodiment of the present invention, the lifting power mechanism includes a lifting driving motor, a sliding block, and a ball spline, the first-stage lifting module drives the second-stage lifting module to move up and down through the sliding block, a flange outer ring in the ball spline is connected to the sliding block, a spline nut in the ball spline is connected to a screw rod in the first-stage lifting module and the second-stage lifting module through two sets of synchronous pulley synchronous belts, and the lifting driving motor drives a spline shaft in the ball spline to rotate, so as to synchronously drive the first-stage lifting module and the second-stage lifting module through the spline nut.
The beneficial effects of the invention are: the full-automatic biological sample low-temperature access operation can be realized, the working efficiency and the productivity are effectively improved, the activity of the sample can be effectively ensured, and the sample safety of storage is improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:
FIG. 1 is a schematic view of a first lid opening mechanism in a preferred embodiment of a system for cryogenic storage of biological samples according to the invention;
FIG. 2 is a schematic diagram of the internal structure of a vacuum liquid nitrogen tank in a preferred embodiment of the cryogenic storage system for biological samples of the present invention;
FIG. 3 is a schematic side sectional view of a vacuum liquid nitrogen tank in accordance with a preferred embodiment of the cryogenic storage system for biological samples of the present invention;
FIG. 4 is a schematic diagram of a preferred embodiment of a cryogenic storage system for biological samples according to the present invention;
FIG. 5 is a schematic view of a portion of a second uncapping mechanism according to a preferred embodiment of the invention;
FIG. 6 is a schematic diagram of a multi-stage module according to a preferred embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Referring to fig. 1-6, an embodiment of the present invention includes:
the utility model provides a low temperature storage system for biological sample for carry out low temperature storage to the carrier that is equipped with sample and sample box, and carry the promotion to freezing the frame of depositing, in order to realize freezing the last unloading of depositing the frame, its structure includes: the device comprises a vacuum liquid nitrogen tank 21, a freezing frame outlet (cylindrical structure) 22, a carrying disc discharge port 23, a first cover opening mechanism, a second cover opening mechanism, a lifting heat preservation bin, a freezing frame 25, a freezing frame storage frame rotating mechanism and a lifting discharge mechanism.
The export of the freezing frame is arranged on the top surface of the vacuum liquid nitrogen tank, the discharge hole of the carrying disc is arranged on the side surface of the export of the freezing frame, so that the grabbing height of the carrying disc can be reduced, and the maximization of the storage of the carrying disc realized in the limited height is realized.
The promotion heat preservation storehouse cover is located the top of vacuum liquid nitrogen container to form the outside low temperature region of protection sample, promote discharge mechanism and with freezing frame export cooperation be connected first uncap mechanism sets up on promoting mesa 24 in the heat preservation storehouse, second uncap mechanism set up in carry the outside of dish discharge gate, freezing frame storage frame rotary mechanism with the vacuum liquid nitrogen container is connected to it is rotatory in the vacuum liquid nitrogen container to drive freezing frame.
(1) Frozen frame storage frame rotary mechanism: the freezing rack is used for storing the freezing rack and driving the freezing rack to rotate in the vacuum liquid nitrogen tank to the lower part of an outlet of the freezing rack so as to realize the storage and the taking of different freezing racks.
The storage frame rotating mechanism of the freezing frame comprises a rotating driving mechanism 270, a cantilever shaft 271, a falling frame, a separation plate 273, a hollow connecting groove 274 and a central liquid storage tank 275 connected with an external liquid nitrogen input valve.
The cantilever shaft is rotatably arranged at the top of the vacuum liquid nitrogen tank through a bearing and the like, the lower part of the cantilever shaft is connected with the top of the falling frame so as to suspend the falling frame in the vacuum liquid nitrogen tank, the bottom of the falling frame is not contacted with the bottom of the vacuum liquid nitrogen tank, and the upper end of the cantilever shaft is connected with the rotary driving mechanism so as to drive the falling frame to rotate in the vacuum liquid nitrogen tank. Give up the mode that the frame top that falls and the bottom were connected with vacuum liquid nitrogen container through the bearing is direct respectively among the prior art promptly, top suspended structure has been adopted, connect rotary drive mechanism and jar interior frame that falls respectively through the cantilever shaft, make the key part that freezes a frame storage frame rotary mechanism of depositing be located the top, in order to keep away from the low temperature storage area of vacuum liquid nitrogen container bottom, the external environment of key part has been optimized, the part fault rate has effectively been reduceed, the service life of equipment is prolonged, and the installation is maintained simply, the hardware cost can be simplified.
Wherein, the central shaft of the storage disc adopts an aluminum alloy pipe with a large cross section; the rotary drive mechanism comprises a rotary drive motor and a harmonic reducer.
The position frame that falls includes storage disc center pin 272 and the supporting disk 276 of locating storage disc center pin bottom periphery in the ring, be provided with on the supporting disk with freeze the support guide way that puts up swing joint, be provided with the cavity spread groove that the opening is down in the storage disc center pin, after the pressurization center liquid storage pot set up in the cavity spread groove, and the lower part of center liquid storage pot directly or through catheter 277 and vacuum liquid nitrogen tank and/or vacuum liquid nitrogen tank bottom and the cavity cooling intermediate layer 278 of side be linked together, supplement or carry the liquid nitrogen in the center liquid storage pot to vacuum liquid nitrogen tank bottom and/or in the middle or air cooling intermediate layer. In addition, the central liquid storage tank can also be simultaneously communicated into the liquid nitrogen tank to supplement and recover liquid nitrogen for the tank body.
Through setting up central liquid storage pot, can increase the liquid nitrogen reserves, prolong the liquid feeding cycle to further optimize the inside warm area of vacuum liquid nitrogen container, make the temperature and the bottom of vacuum liquid nitrogen container top position more be close, thereby realize more balanced stable low temperature environment, the effective storage of the sample of being convenient for.
The division board uses cantilever shaft (or storage disc center pin) to set up on the frame of falling position as the centre of a circle array to form a plurality of fan-shaped structure's that are used for placing the frame of freezing and depositing the room that freezes, adopted the storage space in the unique fan-shaped structure segmentation liquid nitrogen bucket promptly, maximize the memory space in usable effective space, improved storage efficiency by a wide margin.
(2.2) freezing and storing rack: used for positioning and storing a plurality of loading disks.
The freezing frame adopts the tower structure that can make a plurality of year dishes range upon range of emission from top to bottom, and its cross-section is fan-shaped structure, can be so that the freezing frame is circular arranging inside the circular holding vessel, can the efficient utilize the inside usage space of storage jar to improve the quantity of storage unit.
The structure of freezing frame includes: body frame, top plate, bottom plate, guide pin 251, support tray, locking post (e.g. round steel etc.).
The roof with the bottom plate set up respectively in main body frame's top surface and bottom surface, the locking post set up in the main body frame for carry the storage location piece on the dish with locking post joint, with the locking location carry the dish, utilize stainless steel thin layer board to separate a plurality of dish storage spaces that carry that are used for placing a year dish with main body frame, be provided with on the bottom plate with support guide way swing joint's in the frame storage frame rotary mechanism that freezes support locating pin and support tray, place the frame that freezes to deposit with accurate efficient, be provided with on the roof with promotion discharging mechanism swing joint's direction straining structure to it can drive the frame up-and-down motion that freezes to promote discharging mechanism.
The guide fastening structure comprises a lifting guide device and a lifting fastening device, the lifting guide device comprises a lifting guide groove or a lifting guide pin, and the lifting fastening device comprises a lifting fastening fixture block or a lifting fastening groove with a convex-shaped cross section.
The top and the bottom can be made of aluminum alloy plates, and the laminates can be connected by bolts, integrally formed or welded in the main body frame.
(2.3) lifting and discharging mechanism: the device is used for grabbing a freezing frame in the vacuum liquid nitrogen tank and driving the freezing frame to move up and down in an outlet of the freezing frame, so that the carrying disc gripper grabs or deposits a carrying disc.
Promote discharge mechanism including freezing frame tongs 260, tongs mount 261, promote driving motor 262, one-level lifting module 263, second grade lifting module 264 for snatch freezing frame tongs and the drive freezing frame tongs driving motor that puts up tongs and open and shut that puts up tongs set up in on the tongs mount, one-level lifting module is fixed to be set up on promoting the mesa in heat preservation storehouse, or slides through horizontal X axle and sets up on promoting the mesa in heat preservation storehouse, and the output of one-level lifting module with second grade lifting module is connected to drive second grade lifting module up-and-down motion, the output of second grade lifting module with the tongs mount is connected to drive freezing frame tongs up-and-down motion. The gripper fixing frame can be further provided with a positioning pin 265 connected with a lifting guide groove on the freezing storage frame.
The grip of the freezing storage frame is connected with the guide fastening structure so as to grip the freezing storage frame. The grabbing and buckling slot is arranged on the top of the freezing storage rack, the lifting module is arranged on the bottom of the freezing storage rack, the grabbing and buckling slot is connected with the lifting module, the lifting module is arranged on the lifting module, and the grabbing and buckling slot is connected with the lifting module.
In addition, the first-level lifting module and the second-level lifting module can adopt screw rod modules.
And the first-stage lifting module and the second-stage lifting module can be respectively connected with a lifting driving motor to be driven independently.
Alternatively, a motor can be used to drive the multi-stage module to move simultaneously, and the structure of the multi-stage module comprises a sliding block 266, a ball spline 267; the sliding block is connected with the second-level lifting module and the nut in the first-level lifting module respectively, so that the first-level lifting module can drive the second-level lifting module to move up and down through the sliding block. In addition, the sliding block can also be connected with the lifting slide rail through the sliding block so as to improve the lifting of accurate control.
The lower part of the spline shaft of the ball spline can be connected with a lifting drive motor 262 through a group of synchronous pulley synchronous belts to drive the spline shaft of the ball spline to rotate on the fixed frame of the one-level lifting module.
The flange outer lane of ball spline with the sliding block is connected, can rotate along with the spline shaft is rotatory, again can be connected with the lead screw in one-level lifting module and the second grade lifting module respectively through two sets of synchronizing wheel hold-in ranges along the spline mother of spline shaft gliding from top to bottom to two sets of lifting module of synchronous drive.
That is, the lead screw that spline female earlier in through a set of synchronizing wheel hold-in range drive one-level lifting module is rotatory (the synchronizing wheel sets up on female and the one-level lead screw 268 of spline), make one-level lifting module can drive the lift of second grade lifting module, then the spline female is rotatory through the lead screw in another set of synchronizing wheel hold-in range drive second grade lifting module again (the synchronizing wheel sets up on female and the second grade lead screw of spline), make second grade lifting module drive and freeze and deposit a tongs lift, just so can utilize a motor drive two-stage straight line module simultaneous movement, realize freezing and deposit a tongs and go deep into the inside function of drawing the frame of freezing of vacuum liquid nitrogen jar, can save the motor setting, compression equipment size, moreover, the steam generator is simple in structure, and the cost is reduced.
(2.4) a first lid opening mechanism: used for sealing or opening the outlet of the freezing storage rack.
The first cover opening mechanism comprises an opening cover 280 movably connected with an outlet of the cryopreservation frame and a first driving mechanism 281 driving the opening cover to lift and rotate.
First actuating mechanism includes first drive mount, a driving motor, opening lid drive screw, opening lid link 282, a drive mount sets up on promoting the mesa in heat preservation storehouse, opening lid drive screw rotate connect in on the first drive mount, a driving motor is connected with opening lid drive screw to drive opening lid drive screw rotates, the one end of opening lid link with opening lid drive screw threaded connection is connected with on the other end the opening lid. When first driving motor during operation, opening lid driving screw passes through opening lid link and drives opening lid elevating movement in the time for opening lid rotates around opening lid driving screw, thereby makes opening lid lifting and drop rotation downwards in order to seal and freeze and deposit a frame export, or upwards lifting and drop rotation in order to open and freeze and deposit a frame export.
(2.5) a second cap opening mechanism: used for opening or closing the discharge hole of the carrier disc.
The second cover opening mechanism comprises a sealing cover 290 and a second driving mechanism for driving the sealing cover to lift and move back and forth, so that the sealing cover can open or seal the discharge hole of the carrying disc.
The second driving mechanism comprises a second driving fixing frame 291, a second driving motor 292, a second synchronizing wheel 293, a second synchronizing belt 294, a transmission connecting frame 295, a transmission connecting rod, a transmission guide wheel (or transmission guide pin) 297, a lifting transmission guide groove 298 and a telescopic transmission guide groove 299.
The second drive mount sets up in the both sides of carrying the dish discharge gate, be provided with a pair of second synchronizing wheel of connecting through the rotation of second hold-in range on one side or the both sides of second drive mount, all be provided with a set ofly on one side or the both sides of second drive mount the lift transmission guide slot with the flexible transmission guide slot, just flexible transmission guide slot set up in the inboard of lift transmission guide slot, the transmission connecting rod is connected with second hold-in range and closing cap respectively, just the transmission connecting rod pass through the transmission leading wheel respectively with lift transmission guide slot and flexible transmission guide slot are connected to go up and down and flexible direction, thereby drive the closing cap and descend and the inward movement carries the dish discharge gate with sealed, or rise and the outward movement in order to open and carry the dish discharge gate, just can accomplish the continuous action that lets the closing cap realize level and smooth lid again promptly with a structure.
The lifting transmission guide groove can be of a linear structure arranged along the vertical direction, and the telescopic transmission guide groove can be of an arc or L-shaped structure extending from the lower end to the direction close to the discharge port of the carrying disc.
The transmission connecting rod includes lift drive portion 2961 and flexible drive portion 2962, the one end of transmission link and the second hold-in range is connected, and the other end is connected with the lift drive portion among the transmission connecting rod, and the one end of flexible drive portion rotates with lift drive portion to be connected, and the other end rotates with the closing cap to be connected, lift drive portion or be connected with lift drive portion the tip of flexible drive portion passes through transmission leading wheel and lift drive guide slot sliding connection to go up and down to lead, make the closing cap up-and-down motion, be connected with the closing cap the tip of flexible drive portion passes through transmission leading wheel and flexible drive guide slot sliding connection, in order to lead telescopically, makes the closing cap be close to or keep away from and carry a set discharge gate.
Two sides of the sealing cover can be respectively connected with a group of second driving motors, and can also be connected with two groups of transmission devices through a synchronizing shaft 2910 to realize bilateral synchronization, namely, two ends of the synchronizing shaft are connected with the second synchronizing wheels through a group of synchronizing wheel synchronous belts, so that the two groups of transmission devices can be synchronously driven by only one second driving motor, the structure is simplified, and the cost is reduced.
The cryogenic storage system for biological samples is operative: the freezing frame storage frame rotating mechanism drives the freezing frame to rotate, so that the target freezing frame is aligned to a freezing frame outlet on the vacuum liquid nitrogen tank; the first cover opening mechanism opens an outlet of the freezing storage rack, the lifting and discharging mechanism descends and grabs the target freezing storage rack, and then the target freezing storage rack is lifted upwards to a certain height, so that a carrying disc on the freezing storage rack is aligned with a carrying disc discharging port; the second cover opening mechanism opens the discharge port of the carrier disc, so that the corresponding carrier disc can be grabbed by external docking equipment to store or take out sample test tubes or reagent boxes; after the operation is finished, the second cover opening mechanism closes the discharge port of the carrying disc, the lifting discharge mechanism drives the target cryopreservation frame to descend to the cryopreservation frame storage frame rotating mechanism, and the first cover opening mechanism closes the export of the cryopreservation frame.
The low-temperature storage system for the biological sample has the advantages that:
1. the structural design of the traditional vacuum liquid nitrogen tank is optimized, and the cantilever shaft enables the vacuum liquid nitrogen tank to have lower failure rate; the inside central liquid storage pot makes the inside temperature of vacuum liquid nitrogen container more even, guarantees the activity of sample, has also increased the liquid nitrogen storage capacity simultaneously and has prolonged liquid nitrogen and change the cycle.
2. The modularized test tube carrying tray with independent fan-shaped division realizes the maximization of the utilization rate of the inner space of the circular vacuum liquid nitrogen tank, and the carrying tray design of the standard module can be matched with the automatic requirement of sample test tubes of any form and size.
3. The height of frame access & exit is deposited to ingenious design freezing reduces design cost for sample memory space greatly increased, greatly reduced unit sample storage cost.
4. The whole system is highly intelligent, can realize full-automatic biological sample access operation, and high security, high flexibility, high efficiency.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A cryogenic storage system for biological samples, comprising: a vacuum liquid nitrogen tank, a freezing frame for placing a carrying disc, a freezing frame outlet for the freezing frame to pass in and out, a carrying disc discharge port for the carrying disc to pass in and out, a first cover opening mechanism for opening or sealing the freezing frame outlet, a second cover opening mechanism for opening or sealing the carrying disc discharge port, a freezing frame storage frame rotating mechanism and a lifting discharge mechanism for driving the freezing frame to move up and down,
the freezing frame outlet is arranged on the upper part of the vacuum liquid nitrogen tank, the carrier plate discharge port is arranged on the side wall of the freezing frame outlet, the freezing frame storage frame rotating mechanism suspends the freezing frame in the vacuum liquid nitrogen tank, and the freezing frame is driven to rotate in the vacuum liquid nitrogen tank to the position below the freezing frame outlet so as to be stored and taken.
2. The system of claim 1, wherein the lift-off incubation cap is disposed over the outlet, the opening and closing mechanism, and the lift-off outlet mechanism to form an outer cryogenic region for protecting the sample.
3. The cryogenic storage system for biological samples according to claim 1, wherein the rotating mechanism of the storage frame of the cryopreservation rack comprises a rotary driving mechanism, a cantilever shaft, a landing frame, a partition plate, hollow connecting grooves and a central liquid storage tank for supplying liquid nitrogen, the lower part of the cantilever shaft is connected with the top of the landing frame to suspend the landing frame in the vacuum liquid nitrogen tank, the upper end of the cantilever shaft is connected with the rotary driving mechanism to drive the landing frame to rotate in the vacuum liquid nitrogen tank, the partition plate array is arranged on the landing frame to form a plurality of cryopreservation spaces for placing the cryopreservation rack, the hollow connecting grooves are arranged in the landing frame, and the central liquid storage tank is arranged in the hollow connecting grooves and conveys the liquid nitrogen in the central liquid storage tank to the bottom of the vacuum liquid nitrogen tank and/or the hollow cooling interlayer at the side of the vacuum liquid nitrogen tank to increase the storage capacity of the liquid nitrogen.
4. The system of claim 3, wherein the landing frame comprises a central axis of the storage tray and a support tray surrounding the bottom of the central axis of the storage tray, the hollow connecting slots are disposed in the central axis of the storage tray, the hollow connecting slots have downward openings, and the partition plates are disposed on the support tray in an array centered on the central axis of the storage tray to form a plurality of freezing spaces for placing fan-shaped structures of the freezing rack.
5. The system of claim 1, wherein the first lid-opening mechanism comprises a lid movably connected to the outlet of the cryopreservation rack, and a first driving mechanism for driving the lid to lift and rotate.
6. The system of claim 1, wherein the second lid opening mechanism comprises a lid and a second driving mechanism for synchronously lifting and moving the lid back and forth to open or seal the carrier plate outlet.
7. The cryogenic storage system for biological samples according to claim 6, wherein the second driving mechanism comprises a second driving fixing frame, a lifting transmission guide groove, a telescopic transmission guide groove, a transmission guide wheel, a transmission connecting rod and a power device, the lifting transmission guide groove and the telescopic transmission guide groove are arranged on the second driving fixing frame, the end portion of the transmission connecting rod is connected with the sealing cover, the transmission connecting rod is movably connected with the lifting transmission guide groove and the telescopic transmission guide groove through the transmission guide wheel respectively so as to lift and telescopically guide the transmission connecting rod and the sealing cover, the power device drives the transmission connecting rod to move along the guide groove, so that the sealing cover descends and inwards approaches to a sealed loading disc discharge port or ascends and outwards keeps away from an opened loading disc discharge port, wherein the lifting transmission guide groove is of a linear structure arranged along a vertical direction, and the telescopic transmission guide groove is of an arc or L-shaped structure with a lower end extending towards a direction approaching the loading disc discharge port.
8. The cryopreservation system for biological samples as claimed in claim 7, wherein the transmission link comprises a lifting transmission part and a telescopic transmission part, one end of the transmission link is connected with the power device, the other end of the transmission link is connected with the lifting transmission part, one end of the telescopic transmission part is rotatably connected with the lifting transmission part, the other end of the telescopic transmission part is rotatably connected with the sealing cover, the lifting transmission part or the telescopic transmission part is slidably connected with the lifting transmission guide groove through the transmission guide wheel for lifting and guiding, so that the sealing cover moves up and down, and the end part of the telescopic transmission part connected with the sealing cover is slidably connected with the telescopic transmission guide groove through the transmission guide wheel for telescopic guiding, so that the sealing cover is close to or far away from the discharge port of the carrying tray.
9. The cryogenic storage system for biological samples according to claim 1, wherein the lifting and discharging mechanism comprises a freezing rack gripper, a gripper fixing frame, a first-stage lifting module and a second-stage lifting module, the freezing rack gripper for gripping the freezing rack is arranged on the gripper fixing frame, the first-stage lifting module is connected with the second-stage lifting module and drives the second-stage lifting module to move up and down, the second-stage lifting module drives the freezing rack gripper to move up and down through the gripper fixing frame, and a lifting power mechanism is connected with the first-stage lifting module and the second-stage lifting module to synchronously drive the multi-stage lifting modules.
10. The cryogenic storage system for biological samples according to claim 9, wherein the lifting power mechanism comprises a lifting driving motor, a sliding block and a ball spline, the first lifting module drives the second lifting module to move up and down through the sliding block, a flange outer ring in the ball spline is connected with the sliding block, a spline nut in the ball spline is respectively connected with a screw rod in the first lifting module and the second lifting module through two sets of synchronous wheels, and the lifting driving motor drives a spline shaft in the ball spline to rotate so as to synchronously drive the first lifting module and the second lifting module through the spline nut.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210733880 | 2022-06-27 | ||
| CN2022107338808 | 2022-06-27 |
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| CN115321064A true CN115321064A (en) | 2022-11-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210776347.XA Pending CN115321064A (en) | 2022-06-27 | 2022-07-04 | Low-temperature storage system for biological samples |
| CN202221691291.XU Active CN217837014U (en) | 2022-06-27 | 2022-07-04 | Low-temperature storage system for biological samples |
| CN202221718658.2U Active CN218199457U (en) | 2022-06-27 | 2022-07-06 | Low-temperature storage mechanism for biological samples |
| CN202221744607.7U Active CN218199966U (en) | 2022-06-27 | 2022-07-08 | Low-temperature environment carrying mechanism for biological samples |
| CN202210811015.0A Pending CN115108218A (en) | 2022-06-27 | 2022-07-11 | Biological sample low temperature intelligent storage system |
| CN202221777140.6U Active CN217837016U (en) | 2022-06-27 | 2022-07-11 | Biological sample low temperature intelligence storage system |
| CN202221790978.9U Active CN217837545U (en) | 2022-06-27 | 2022-07-13 | Low-temperature intelligent access mechanism for biological samples |
| CN202221813998.3U Active CN217837546U (en) | 2022-06-27 | 2022-07-15 | A access call mechanism for biological sample carries dish |
| CN202221833820.5U Active CN217837486U (en) | 2022-06-27 | 2022-07-18 | Automatic low-temperature butt-joint feeding mechanism for biological samples |
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| CN202221718658.2U Active CN218199457U (en) | 2022-06-27 | 2022-07-06 | Low-temperature storage mechanism for biological samples |
| CN202221744607.7U Active CN218199966U (en) | 2022-06-27 | 2022-07-08 | Low-temperature environment carrying mechanism for biological samples |
| CN202210811015.0A Pending CN115108218A (en) | 2022-06-27 | 2022-07-11 | Biological sample low temperature intelligent storage system |
| CN202221777140.6U Active CN217837016U (en) | 2022-06-27 | 2022-07-11 | Biological sample low temperature intelligence storage system |
| CN202221790978.9U Active CN217837545U (en) | 2022-06-27 | 2022-07-13 | Low-temperature intelligent access mechanism for biological samples |
| CN202221813998.3U Active CN217837546U (en) | 2022-06-27 | 2022-07-15 | A access call mechanism for biological sample carries dish |
| CN202221833820.5U Active CN217837486U (en) | 2022-06-27 | 2022-07-18 | Automatic low-temperature butt-joint feeding mechanism for biological samples |
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| CN115557242B (en) * | 2022-12-05 | 2023-03-03 | 基点生物科技(成都)有限公司 | Biological sample access device |
| CN116576950A (en) * | 2023-07-07 | 2023-08-11 | 时新(上海)产品设计有限公司 | Batch weighing device |
| CN117104753B (en) * | 2023-08-25 | 2024-05-17 | 上海药明康德新药开发有限公司 | CRO field compound container management system and container warehouse-in, warehouse-out and inventory method |
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| CN217837014U (en) | 2022-11-18 |
| CN217837016U (en) | 2022-11-18 |
| CN115108218A (en) | 2022-09-27 |
| CN217837486U (en) | 2022-11-18 |
| CN217837546U (en) | 2022-11-18 |
| CN218199457U (en) | 2023-01-03 |
| CN218199966U (en) | 2023-01-03 |
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