CN115300784A - Micro-fluidic chip for substance delivery - Google Patents
Micro-fluidic chip for substance delivery Download PDFInfo
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- CN115300784A CN115300784A CN202210945284.6A CN202210945284A CN115300784A CN 115300784 A CN115300784 A CN 115300784A CN 202210945284 A CN202210945284 A CN 202210945284A CN 115300784 A CN115300784 A CN 115300784A
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- 239000000126 substance Substances 0.000 title claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 36
- 239000012528 membrane Substances 0.000 claims abstract description 34
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 238000004891 communication Methods 0.000 claims description 15
- 239000002184 metal Substances 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 abstract description 13
- 210000000170 cell membrane Anatomy 0.000 abstract description 3
- 150000003904 phospholipids Chemical class 0.000 abstract description 3
- 210000003491 skin Anatomy 0.000 description 26
- 238000004088 simulation Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000009826 distribution Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000002537 cosmetic Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000005684 electric field Effects 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
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- 230000003796 beauty Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
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- 238000009434 installation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000004927 skin cell Anatomy 0.000 description 2
- 206010048038 Wound infection Diseases 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000002716 delivery method Methods 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005489 elastic deformation Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
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- 238000011002 quantification Methods 0.000 description 1
- 206010040872 skin infection Diseases 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M2037/0007—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin having means for enhancing the permeation of substances through the epidermis, e.g. using suction or depression, electric or magnetic fields, sound waves or chemical agents
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Medical Informatics (AREA)
- Anesthesiology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a microfluidic chip for substance delivery, comprising: the chip comprises a chip main body, a first electrode and a second electrode, wherein the chip main body is provided with an inner cavity for containing liquid to be introduced, and a first end of the chip main body is provided with a sample adding port communicated with the inner cavity for injecting the liquid to be introduced; a sample adding cover for sealing the sample adding port; an electrode mounted on the chip body, one end of the electrode extending to the inner cavity; the second end of the chip main body is provided with a communicating hole communicated with the inner cavity, the nanopore membrane is packaged at the second end of the chip main body and is used for being contacted with the part to be led in, when the electrode is electrified, a closed loop can be formed by the nanopore membrane, the microfluidic chip and the part to be led in so as to drive charged substances to be led in liquid to be delivered into the part to be led in through the communicating hole and the nanopore membrane, the cell membrane of a cell phospholipid bilayer in skin is opened by instantaneous voltage, charged molecules to be led in liquid are delivered into the cell from the outside, the liquid to be led in can be led in without puncturing the skin, and the leading-in efficiency is improved.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a micro-fluidic chip for delivering substances.
Background
Beauty instruments are popular because of their ability to modify and improve the condition of the skin, and their main purpose and principle is to achieve their effect by introducing specific cosmetic essence molecules into the skin and cells.
At present, the commonly used introduction method is generally a microneedle, which destroys the skin structure and cell tissue by the damage of needle-like structure with small size, and then permeates the beauty essence into the skin and cell by the smearing method. However, this method requires a risk of damaging the skin, which may result in damage to the skin and failure to repair the skin, or wound infection, and also has relatively limited permeation efficiency of the cosmetic essence, relatively low efficiency, and failure to quantitatively introduce the cosmetic essence.
Disclosure of Invention
In view of the above, the present invention provides a micro fluidic chip for delivering a substance, which aims to improve the introduction efficiency on the basis of avoiding damage to the skin.
In order to achieve the above object, the present invention provides the following solutions:
a microfluidic chip for substance delivery, comprising:
the chip comprises a chip main body, a first electrode and a second electrode, wherein the chip main body is provided with an inner cavity for containing liquid to be introduced, and a first end of the chip main body is provided with a sample adding port communicated with the inner cavity for injecting the liquid to be introduced;
a sample adding cover for plugging the sample adding port;
the electrode is arranged on the chip main body, and one end of the electrode extends to the inner cavity;
the second end of the chip main body is provided with a communicating hole communicated with the inner cavity, the nanopore membrane is packaged at the second end of the chip main body and is used for being contacted with a part to be led in, and when the electrode is electrified, a closed loop can be formed by the nanopore membrane, the microfluidic chip and the part to be led in so as to drive the charged substances in the liquid to be led in to be delivered into the part to be led in through the communicating hole and the nanopore membrane.
In a specific embodiment, the first end of the chip main body is further provided with an air outlet communicated with the inner cavity, and the air outlet is packaged with a waterproof breathable film.
In another specific embodiment, the chip body comprises a cavity and a support plate;
one end of the cavity is arranged in a closed manner and is the first end of the chip main body;
the other end of the cavity is opened and is a second end of the chip main body, the support plate blocks the opened end of the cavity, and the communication hole is formed in the support plate;
the nanopore membrane is fixed on the end face of the open end of the cavity, and a preset gap is reserved between the nanopore membrane and the support plate.
In another specific embodiment, the number of the communication holes is multiple, and the communication holes are uniformly distributed on the support plate.
In another specific embodiment, the inner wall of the second end of the cavity is provided with a limiting step for limiting and abutting against the end surface of the supporting plate;
the end of the supporting plate facing the cavity is provided with a connecting part so as to be detachably connected with the inner wall of the cavity; or the supporting plate and the limiting step are welded by laser.
In another specific embodiment, the connecting part comprises a connecting plate and a clamping protrusion arranged on the connecting plate, the connecting plate is fixed on the supporting plate, and the inner wall of the cavity is provided with a clamping groove clamped with the clamping protrusion;
or
The connecting part comprises a connecting plate and a clamping groove formed in the connecting plate, and a clamping protrusion used for being clamped with the clamping groove is arranged on the inner wall of the cavity;
or
The connecting part is a circular plate fixed on the supporting plate, external threads are formed in the circular plate, the inner cavity is a cylindrical cavity, and internal threads matched with the external threads in a threaded mode are formed in the inner cavity.
In another specific embodiment, the side wall of the supporting plate is provided with a limited rotation guide groove, and the inner wall of the cavity is provided with a limited rotation guide protrusion limited in the limited rotation guide groove.
In another specific embodiment, the end of the supporting plate facing the inner cavity is further provided with a guide plate, and the side surface of the guide plate facing the inner cavity is flush with the groove bottom of the guide groove and is attached to the guide protrusion to slide.
In another specific embodiment, the outer wall of the cavity is provided with a sliding groove in sliding fit with the piece to be installed, the side wall of the cavity is recessed towards the inner wall of the cavity, the sliding groove is formed on the outer side, and the guide protrusion is formed on the inner wall;
the cavity is further provided with a clamping groove which is connected with the mounting piece in a clamping mode.
In another specific embodiment, the sample addition cover comprises a fastening cover body, an elastic cover body and a connecting piece, two ends of the connecting piece are respectively connected with the fastening cover body and the elastic cover body, a fastening hole is formed in the chip main body, the fastening cover body is installed in the fastening hole, and the elastic cover body is detachably and hermetically installed in the sample addition port;
and/or
The electrode is an inert metal electrode;
and/or
The electrode comprises an electrode table and an electrode column, the electrode column is vertically connected with the electrode table, the end surface of the electrode table surface facing the electrode column is hermetically connected with the accommodating groove, and the electrode column penetrates through the accommodating hole and extends out of the accommodating hole by a preset length;
and/or
The gas exhaust port is a counter bore, and the waterproof breathable film is connected with the chip main body through ultrasonic bonding;
and/or
The nanopore membrane is laser welded to the chip body.
The various embodiments according to the invention can be combined as desired, and the embodiments obtained after these combinations are also within the scope of the invention and are part of the specific embodiments of the invention.
Taking the part to be introduced as human skin as an example, when the micro-fluidic chip provided by the invention is used, the sample adding cover is opened, the liquid to be introduced is injected into the inner cavity of the chip main body through the sample adding opening, after the sample adding is finished, the sample adding cover is covered, the nano-pore membrane of the micro-fluidic chip is attached to the human skin, the electrode is electrified through the power supply piece, so that the micro-fluidic chip, the skin and the cells form a closed loop, the cell membrane of the cell phospholipid bilayer in the skin is opened by instantaneous voltage, the charged molecules in the liquid to be introduced are conveyed into the cells from the outside, the liquid to be introduced can be introduced into the skin and the cells without puncturing the skin, and the introduction efficiency is improved. Namely, the invention improves the leading-in efficiency on the basis of avoiding the damage to the skin.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without novelty work.
Fig. 1 is a schematic diagram of a three-dimensional structure of a microfluidic chip provided by the present invention in one angular direction;
FIG. 2 is a schematic diagram of a three-dimensional structure of a microfluidic chip provided by the present invention in another angular direction;
FIG. 3 is a schematic diagram of a three-dimensional structure of a microfluidic chip provided by the present invention without a nanopore membrane;
fig. 4 is a schematic diagram of an exploded structure of a microfluidic chip provided by the present invention;
FIG. 5 is a schematic cross-sectional view of a microfluidic chip according to the present invention;
FIG. 6 is a schematic cross-sectional view of a microfluidic chip according to the present invention;
FIG. 7 is a graph showing the simulation result of the internal voltage trend distribution of the microfluidic chip according to the present invention;
fig. 8 is a diagram showing a simulation result of the internal current density distribution of the microfluidic chip according to the present invention.
Wherein, in fig. 1-8:
the micro-fluidic chip comprises a micro-fluidic chip 1000, a chip main body 100, an inner cavity 101, a sample adding port 102, a sample adding cover 200, an electrode 300, a nano-pore membrane 400, a notch 401, a communication hole 103, an exhaust port 104, a waterproof breathable membrane 500, a cavity 105, a support plate 106, a limiting step 105a, a connecting part 106a, a connecting plate 106a-1, a clamping protrusion 106a-2, a clamping groove 105b, a rotation limiting guide groove 106b, a rotation limiting guide protrusion 105c, a guide plate 106c, a sliding groove 105d, a clamping groove 105e, a fastening cover body 201, an elastic cover body 202, a connecting piece 203, a fastening hole 107, an accommodating groove 108, an accommodating hole 108a, an electrode platform 301, an electrode column 302, a chip model 600 and a skin cell layer 700.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to fig. 1 to 8 in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "top", "bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the position or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Referring to fig. 1 to 6, the present invention provides a micro-fluidic chip 1000 for delivering a substance, and the micro-fluidic chip 1000 can be used to achieve efficient, rapid and accurate delivery of a charged medium through a nanopore on a nanopore membrane 400 under the action of an electric field, and compared with other delivery methods and consumables, the present invention can achieve accurate quantification and accurate delivery, and has a good uniformity, and the charged medium can effectively break through the stratum corneum of the skin to reach the dermis of the skin without damage, so as to achieve the purpose of repair and treatment to avoid damage to the skin, and improve the introduction efficiency.
As shown in fig. 1, fig. 2 and fig. 4, the microfluidic chip 1000 includes a chip main body 100, a sample-adding cover 200, an electrode 300 and a nanopore membrane 400, where the chip main body 100 has an inner cavity 101 for holding a liquid to be introduced, and specifically, the shape of the inner cavity 101 is not limited, and may be set as the inner cavity 101 with any shape as required. For the convenience of processing and manufacturing, the invention discloses that the chip main body 100 is in a circular cylindrical shape, and correspondingly, the inner cavity 101 is in a cylindrical shape. It should be understood that the shape of the chip body 100 disclosed above is only one embodiment of the present invention, and in practical applications, the chip body 100 may be a tube with other regular shapes such as a square shape, or a profile structure with other irregular shapes.
The first end of the chip main body 100 is opened with a sample injection port 102 communicating with the inner cavity 101 for injecting a liquid to be introduced. It should be noted that the sample addition port 102 may be a circular hole, a square hole, or the like, and the specific shape is not limited. The liquid to be introduced can be cosmetic essence or other medicaments, and can be selected according to the needs.
In order to further improve the sealing performance of the sample adding cover 200 and the sample adding port 102, the invention discloses that the sample adding port 102 comprises an equal-diameter hole and a gradually-expanded hole, wherein one end of the equal-diameter hole is connected with the smaller end of the gradually-expanded hole, the other end of the equal-diameter hole is communicated with the inner cavity 101, the larger end of the gradually-expanded hole is positioned at the end face of the first end of the chip main body 100, the wall surface of the gradually-expanded hole is abutted to the sample adding cover 200, the area of a sealing surface is increased, and the sealing performance of the sample adding cover 200 and the sample adding port 102 is further improved.
The electrode 300 is mounted on the chip body 100, and one end of the electrode 300 extends to the inner cavity 101, specifically, the electrode 300 can be electrically connected to a power supply to achieve energization.
As shown in fig. 3, the nanopore membrane 400 is packaged at the second end of the chip main body 100, and is used for contacting with a portion to be introduced, and when the electrode 300 is powered on, a closed loop can be formed with the microfluidic chip 1000 and the portion to be introduced, so as to drive the charged substance to be introduced into the liquid to be delivered into the portion to be introduced through the communication hole 103 and the nanopore membrane 400. Specifically, the portion to be introduced is human skin or the like.
When the microfluidic chip 1000 provided by the present invention is used, the microfluidic chip 1000 may be used as an introduction head of an introduction instrument, and may be mounted on the introduction instrument, or may be used alone. Here, it is understood that the introducer is a device capable of mounting the microfluidic chip 1000 and turning on/off the electrodes 300 of the microfluidic chip 1000, and the specific structure and shape are not limited and may be set as necessary.
When liquid to be introduced is required to be introduced into human skin, the sample adding cover 200 is opened, the liquid to be introduced is injected into the inner cavity 101 of the chip main body 100 through the sample adding port 102, after sample adding is finished, the sample adding cover 200 is covered, the nanopore membrane 400 of the microfluidic chip 1000 is attached to the human skin, the electrode 300 is electrified through the power supply piece, the microfluidic chip 1000, the skin and cells form a closed loop, the cell membrane of a cell phospholipid bilayer in the skin is opened through instantaneous voltage, charged molecules in the liquid to be introduced are conveyed into the cells from the outside, the liquid to be introduced can be introduced into the skin and the cells without puncturing the skin, and the introduction efficiency is improved. Namely, the invention improves the introduction efficiency on the basis of avoiding the damage to the skin.
In some embodiments, the first end of the chip body 100 further defines an exhaust port 104 communicating with the inner cavity 101, so as to facilitate discharging the gas in the inner cavity 101 when the liquid is added to the inner cavity 101, thereby preventing the gas from being mixed in the liquid to be introduced.
In order to prevent the liquid to be introduced in the inner cavity 101 from overflowing from the exhaust port 104, the invention discloses that the waterproof breathable film 500 is packaged on the exhaust port 104.
Further, the present invention discloses that the gas outlet 104 is a counterbore, and the waterproof breathable film 500 is connected in the counterbore by ultrasonic bonding. It should be noted that the waterproof breathable film 500 and the counter bore are not limited to be connected by ultrasonic bonding, but may be welded or bonded, and the specific connection manner is not limited, and all connection manners that can fix the waterproof breathable film 500 in the counter bore are within the protection scope of the present invention.
In addition, waterproof ventilated membrane 500 also can be used for observing whether the application of sample is accomplished, in the application of sample process, wait to introduce liquid and get into inner chamber 101 from application of sample mouth 102, because the action of gravity, wait to introduce liquid and subside on nanopore membrane 400, and the gas of inner chamber 101 is discharged through gas vent 104, along with the going on of application of sample, when waiting to introduce liquid and fill up whole inner chamber 101, the gas in the inner chamber 101 is all discharged, pauses after the liquid level contacts waterproof ventilated membrane 500 to accomplish whole application of sample.
On one hand, the micro-fluidic chip 1000 has high manufacturing precision, and the dimensional precision of the inner cavity 101 for containing the liquid to be introduced is improved; on the other hand, in the application of sample in-process, whether the application of sample is accomplished through observing waterproof ventilated membrane 500 convenience in time, avoided adding the not full condition, further improved the accurate ration of waiting to leading-in liquid.
In some embodiments, the chip main body 100 includes a cavity 105 and a supporting plate 106, one end of the cavity 105 is closed and is a first end of the chip main body 100, the other end of the cavity 105 is open, and is a second end of the chip main body 100, the supporting plate 106 closes the open end of the cavity 105, and the communication hole 103 is opened on the supporting plate 106. The chip main body 100 is split into the cavity 105 and the supporting plate 106, so that the processing difficulty is reduced, and the batch production is facilitated.
The nanopore membrane 400 is fixed on the end face of the open end of the cavity 105 with a predetermined gap from the support plate 106. It should be noted that the preset gap is specifically set according to needs, and is not limited to a specific value. Through the setting of predetermined clearance for the liquid that treats that the intercommunicating pore 103 came out can the equipartition on nanopore membrane 400, and the nanopore membrane 400 of being convenient for will treat that the even leading-in of leading-in liquid treats the leading-in.
Furthermore, the invention discloses that the number of the communication holes 103 is multiple, and the communication holes are uniformly distributed on the support plate 106, so that the liquid to be introduced in the inner cavity 101 can rapidly and uniformly enter the preset gap through the communication holes 103.
Furthermore, the invention discloses that the inner wall of the second end of the cavity 105 is provided with a limit step 105a for limiting and abutting against the end face of the support plate 106, so that the support plate 106 is convenient to mount, in addition, the contact area of the limit step 105a and the support plate 106 is increased through the abutting of the limit step 105a and the support plate 106, and the sealing effect is further improved.
The end of the supporting plate 106 facing the cavity 105 is provided with a connecting portion 106a to detachably connect with the inner wall of the cavity 105, so as to facilitate disassembly and replacement, it should be noted that the detachable connection of the supporting plate 106 with the cavity 105 through the connecting portion 106a is only a specific embodiment of the present invention, and in practical applications, the supporting plate 106 and the limiting step 105a may also be provided to realize the non-detachable connection of the supporting plate 106 with the cavity 105 through laser welding and other manners.
Taking the detachable connection of the supporting portion with the cavity 105 through the connecting portion 106a as an example, specifically, the connecting portion 106a includes a connecting plate 106a-1 and a clamping protrusion 106a-2 disposed on the connecting plate 106a-1, the connecting plate 106a-1 is fixed on the supporting plate 106, and a clamping groove 105b clamped with the clamping protrusion 106a-2 is disposed on an inner wall of the cavity 105, as shown in fig. 5 and 6. In order to realize the stable connection between the support plate 106 and the cavity 105, the invention discloses that the number of the connecting parts 106a is at least 2, and the connecting parts are annularly and uniformly distributed on the support plate 106. Of course, the connecting portion 106a may also include a connecting plate 106a-1 and a clamping groove 105b formed on the connecting plate 106a-1, and the inner wall of the cavity 105 is provided with a clamping protrusion 106a-2 for clamping with the clamping groove 105 b.
Further, the invention discloses that the clamping protrusion 106a-2 is matched with the clamping groove 105b in shape, specifically, the clamping protrusion 106a-2 is an arc-shaped protrusion, and correspondingly, the clamping groove 105b is an arc-shaped groove.
The above-mentioned detachable connection manner of the connection portion 106a and the cavity 105 is only one specific embodiment of the present invention, and in practical applications, other connection manners are also possible, for example, the connection portion 106a is a circular plate fixed on the support plate 106, and the circular plate is provided with an external thread, the inner cavity 101 is a cylindrical cavity, and the inner cavity 101 is provided with an internal thread matching with the external thread. That is, the connecting portion 106a is detachably connected to the inner cavity 101 by a screw connection.
Furthermore, the invention discloses that the side wall of the supporting plate 106 is provided with a limited rotation guide groove 106b, and the inner wall of the cavity 105 is provided with a limited rotation guide protrusion 105c limited in the limited rotation guide groove 106 b.
According to the invention, the rotation limiting protrusion is matched with the rotation limiting guide groove 106b, so that on one hand, the clamping protrusion 106a-2 of the support plate 106 is conveniently clamped into the clamping groove 105b on the inner wall of the cavity 105, on the other hand, the support plate 106 is prevented from rotating relative to the cavity 105, and the clamping stability of the support plate 106 and the cavity 105 is further improved.
Furthermore, the invention discloses that the end of the support plate 106 facing the inner cavity 101 is also provided with a guide plate 106c, and the side surface of the guide plate 106c facing the inner cavity 101 is flush with the groove bottom of the guide groove and is attached to the guide protrusion for sliding.
That is, the guide plate 106c is provided to facilitate alignment of the support plate 106 with the cavity 101, and to facilitate guiding of the support plate 106.
In some embodiments, the outer wall of the cavity 105 is provided with a sliding groove 105d which is in sliding fit with the component to be installed, the side wall of the cavity 105 is recessed towards the inner wall of the cavity 105, the outer side of the cavity forms the sliding groove 105d, and the inner wall forms a guide protrusion, so that the processing and forming are facilitated. Of course, it is understood that the slide groove 105d and the guide protrusion may be separately provided along the circumferential direction of the cavity 105.
The cavity 105 is further provided with a clamping groove 105e clamped with the to-be-mounted piece, so that the micro-fluidic chip 1000 and the to-be-mounted piece can be conveniently disassembled and assembled. It should be noted that, when the microfluidic chip 1000 is used as an introduction head of an introduction instrument, the component to be mounted is a main body of the introduction instrument. When the microfluidic chip 1000 needs to be mounted on a bracket or a housing for handheld use, the component to be mounted is the bracket or the housing.
Further, the invention discloses that the nanopore membrane 400 and the chip body 100 are welded by laser, and specifically, the nanopore membrane 400 is provided with a notch 401 corresponding to the sliding groove 105d to avoid interference with the installation of the microfluidic chip 1000.
In some embodiments, the sample addition cover 200 includes a fastening cover 201, an elastic cover 202, and a connector 203, two ends of the connector 203 are respectively connected to the fastening cover 201 and the elastic cover 202, the chip main body 100 is provided with a fastening hole 107, the fastening cover 201 is installed in the fastening hole 107, and the elastic cover 202 is detachably and hermetically installed in the sample addition port 102. During the use, only need to pull out elasticity lid 202 from the application of sample mouth 102 in can carry out the application of sample, because elasticity lid 202 passes through connecting piece 203 and is connected with fastening lid 201, avoided the circumstances that elasticity lid 202 loses.
The fastening cover body 201 and the connector 203 may be made of the same material as the elastic cover body 202, or may be different from each other, and may be specifically set as needed.
Of course, the fastening hole 107 may be a blind hole or a through hole, and when the fastening hole 107 is a through hole, it can also be used as a sample application.
It can be understood that the above-disclosed specific structure of the sample-adding cover 200 is only one specific embodiment of the present invention, and in practical applications, the sample-adding cover 200 may also include only one elastic cover, the elastic cover is detachably mounted at the sample-adding port 102, and for convenience of assembly and disassembly, a pull ring or a handle may be disposed on the elastic cover.
In some embodiments, the first end of the chip body 100 is provided with a receiving groove 108, the bottom end of the receiving groove 108 is opened with a receiving hole 108a communicated with the inner cavity 101, and the axis of the receiving hole 108a coincides with the center of the chip body 100, the electrode 300 includes an electrode stage 301 and an electrode column 302, the electrode column 302 is vertically connected with the electrode stage 301, the end surface of the electrode stage 301 facing the electrode column 302 is hermetically connected with the receiving groove 108, and the electrode column 302 passes through the receiving hole 108a and extends out of the receiving hole 108a by a preset length. The size and the position distribution of the electrodes 300 on the microfluidic chip 1000 are obtained by performing accurate simulation and calculation based on an electric field, so that the resistance value of the whole system can be ensured to be minimum, and thus the charged medium can be efficiently transmitted inside the chip, the nanopore membrane 400 and the skin cells of the human body. When the device is used, simulation calculation is carried out according to the conductivity of different liquids to be introduced, and electric field parameters are determined. As shown in fig. 7 and 8, fig. 7 is a graph showing a simulation result of a voltage trend distribution inside the microfluidic chip 1000, and fig. 8 is a graph showing a simulation result of a current density distribution inside the microfluidic chip 1000.
Further, the invention discloses that the electrode 300 is an inert metal electrode, which ensures good conductivity, and simultaneously ensures that the metal electrode has good inertia and is not electrolyzed, and has good stability. For example, the electrode 300 is an electrode 300 made of gold or the like.
It should be noted that, in the present specification, words indicating orientation, such as up, down, left, right, and the like, are set in the direction of fig. 1, and are used for convenience of description only, and do not have other specific meanings.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (10)
1. A microfluidic chip (1000) for substance delivery, comprising:
the chip comprises a chip main body (100), wherein the chip main body (100) is provided with an inner cavity (101) for containing liquid to be introduced, and a first end of the chip main body (100) is provided with a sample adding port (102) communicated with the inner cavity (101) for injecting the liquid to be introduced;
a sample addition cover (200) for sealing the sample addition port (102);
an electrode (300), the electrode (300) being mounted on the chip body (100), and one end of the electrode (300) extending to the inner cavity (101);
the second end of the chip main body (100) is provided with a communication hole (103) communicated with the inner cavity (101), the nanopore membrane (400) is packaged at the second end of the chip main body (100) and is used for being in contact with a part to be led in, and when the electrode (300) is electrified, a closed loop can be formed between the nanopore membrane and the microfluidic chip and the part to be led in, so that the charged substances in the liquid to be led in are driven to be delivered into the part to be led in through the communication hole (103) and the nanopore membrane (400).
2. The microfluidic chip (1000) according to claim 1, wherein the first end of the chip body (100) further defines an air outlet (104) in communication with the inner cavity (101), and the air outlet (104) is packaged with a waterproof and breathable film (500).
3. The microfluidic chip (1000) of claim 2, wherein the chip body (100) comprises a cavity (105) and a support plate (106);
one end of the cavity (105) is arranged in a closed manner and is a first end of the chip main body (100);
the other end of the cavity (105) is opened and is a second end of the chip main body (100), the support plate (106) seals the opened end of the cavity (105), and the communication hole (103) is formed in the support plate (106);
the nanopore membrane (400) is fixed on the end face of the open end of the cavity (105), and a preset gap is reserved between the nanopore membrane and the support plate (106).
4. The microfluidic chip (1000) according to claim 3, wherein the number of the communication holes (103) is plural and the communication holes are uniformly distributed on the support plate (106).
5. The microfluidic chip (1000) according to claim 3, wherein the inner wall of the second end of the cavity (105) is provided with a limiting step (105 a) for limiting and abutting against the end face of the support plate (106);
the end of the supporting plate (106) facing the cavity (105) is provided with a connecting part (106 a) for detachably connecting with the inner wall of the cavity (105); or the supporting plate (106) and the limiting step (105 a) are welded by laser.
6. The microfluidic chip (1000) according to claim 5, wherein the connecting portion (106 a) comprises a connecting plate (106 a-1) and a clamping protrusion (106 a-2) disposed on the connecting plate (106 a-1), the connecting plate (106 a-1) is fixed on the supporting plate (106), and a clamping groove (105 b) clamped with the clamping protrusion (106 a-2) is formed on an inner wall of the cavity (105); or
The connecting part (106 a) comprises a connecting plate (106 a-1) and a clamping groove (105 b) formed in the connecting plate (106 a-1), and a clamping protrusion (106 a-2) used for being clamped with the clamping groove (105 b) is arranged on the inner wall of the cavity (105);
or alternatively
The connecting portion (106 a) is a circular ring plate fixed on the supporting plate (106), an external thread is formed in the circular ring plate, the inner cavity (101) is a cylindrical cavity, and an internal thread matched with the external thread in a threaded mode is formed in the inner cavity (101).
7. The microfluidic chip (1000) according to claim 3, wherein the side wall of the supporting plate (106) is provided with a rotation-limiting guide groove (106 b), and the inner wall of the cavity (105) is provided with a rotation-limiting guide protrusion (105 c) limited in the rotation-limiting guide groove (106 b).
8. The microfluidic chip (1000) according to claim 7, wherein the end of the support plate (106) facing the inner cavity (101) is further provided with a guide plate (106 c), and the side of the guide plate (106 c) facing the inner cavity (101) is flush with the bottom of the guide groove (106 b) and slides in abutment with the guide protrusion.
9. The microfluidic chip (1000) according to claim 4, wherein the outer wall of the cavity (105) is provided with a sliding groove (105 d) in sliding fit with the component to be mounted, the side wall of the cavity (105) is recessed towards the inner wall of the cavity (105), the outer side forms the sliding groove (105 d), and the inner wall forms the guide protrusion;
the cavity (105) is further provided with a clamping groove (105 e) which is clamped with the to-be-installed part.
10. The microfluidic chip (1000) according to any of claims 1 to 9, wherein the sample addition cover (200) comprises a fastening cover body (201), an elastic cover body (202), and a connector (203), two ends of the connector (203) are respectively connected to the fastening cover body (201) and the elastic cover body (202), the chip main body (100) is provided with a fastening hole (107), the fastening cover body (201) is installed in the fastening hole (107), and the elastic cover body (202) is detachably and hermetically installed in the sample addition port (102);
and/or
The electrode (300) is an inert metal electrode;
and/or
The first end of the chip main body (100) is provided with an accommodating groove (108), the bottom end of the accommodating groove (108) is provided with an accommodating hole (108 a) communicated with the inner cavity (101), the axis of the accommodating hole coincides with the center of the chip main body (100), the electrode (300) comprises an electrode table (301) and an electrode column (302), the electrode column (302) is vertically connected with the electrode table (301), the end face, facing the electrode column (302), of the electrode table (301) is in sealing connection with the accommodating groove (108), and the electrode column (302) penetrates through the accommodating hole and extends out of the accommodating hole by a preset length;
and/or
The air outlet (104) is a counter bore, and the waterproof breathable film (500) is connected in the counter bore through ultrasonic bonding;
and/or
The nanopore membrane (400) is laser welded to the chip body (100).
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| CN202210945284.6A CN115300784B (en) | 2022-08-08 | 2022-08-08 | Microfluidic chip for substance delivery |
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| CN202210945284.6A CN115300784B (en) | 2022-08-08 | 2022-08-08 | Microfluidic chip for substance delivery |
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| WO2022140984A1 (en) * | 2020-12-28 | 2022-07-07 | 深圳由莱智能电子有限公司 | Cosmetic handle |
| CN115282478A (en) * | 2022-08-08 | 2022-11-04 | 北京载愈生物科技有限公司 | Delivery system and delivery method |
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| JP2015097923A (en) * | 2015-03-04 | 2015-05-28 | 日立マクセル株式会社 | Beauty instrument |
| KR20170138847A (en) * | 2016-06-08 | 2017-12-18 | 주식회사 아모센스 | A skin care device |
| CN110997058A (en) * | 2017-06-28 | 2020-04-10 | 技术研究与创新基金会 | Device and method for controlled and monitored transdermal administration of active agents and use thereof |
| CN213048984U (en) * | 2020-04-14 | 2021-04-27 | 成都柔电云科科技有限公司 | Portable ion leading-in equipment |
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| CN115300784B (en) | 2024-07-05 |
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