CN115287169B

CN115287169B - Detection device based on finger insertion electrode and working method and processing method thereof

Info

Publication number: CN115287169B
Application number: CN202211204903.2A
Authority: CN
Inventors: 关国良; 陈巧玲; 阿布卡尔; 金诚
Original assignee: Changzhou Xianxu Medical Technology Co ltd
Current assignee: Changzhou Xianxu Medical Technology Co ltd
Priority date: 2022-09-30
Filing date: 2022-09-30
Publication date: 2022-12-20
Anticipated expiration: 2042-09-30
Also published as: CN115287169A

Abstract

The invention belongs to the technical field of electrochemical detection, and particularly relates to a detection device based on an interdigitated electrode, and a working method and a processing method thereof. This detection device based on finger electrode includes: the device comprises an amplification cavity, a detection cavity, a flushing liquid cavity and an air blowing interface; the flushing liquid in the flushing liquid cavity is suitable for being conveyed into the detection cavity by an external power source so as to flush the hybridized finger inserting electrode; the blowing interface is suitable for an external air source to blow the flushed finger inserting electrodes through the fourth channel; the flushing liquid in the flushing liquid cavity is suitable for being conveyed to the detection cavity after being dried by an external power source, so that the electrical detection is carried out on the finger inserting electrode. The detection device based on the finger insertion electrode integrates the steps required in the gene detection process of the finger insertion electrode into a chip, can realize that the detection method is operated outside a laboratory, and can effectively reduce the pollution of external genes.

Description

Detection device based on finger insertion electrode and working method and processing method thereof

Technical Field

The invention belongs to the technical field of electrochemical detection, and particularly relates to a detection device based on an interdigitated electrode, and a working method and a processing method thereof.

Background

Interdigitated electrodes are commonly used in microwave filters, surface acoustic wave devices, electro-optical shutters, affinity biosensors, and the like. The finger electrode is used for molecule (gene) detection, and the specific principle is that a biological probe, usually a small segment of single-stranded DNA, is bound on the electrode, and then the electrochemical impedance of the electrode is changed after the probe is hybridized with a target gene. The detection method does not need an additional electrochemical label, does not need a measuring reference line, and is much simpler compared with the traditional method of adding the redox marker.

At present, the method is still in a laboratory stage, manual operation is generally adopted in each step in the measurement process, different instruments and equipment are needed, and detection outside the laboratory cannot be realized.

Disclosure of Invention

The invention aims to provide a detection device based on an interdigital electrode, a working method and a processing method thereof, so that steps required in the gene detection process of the interdigital electrode are integrated into a chip, the detection method can be operated outside a laboratory, and the pollution of external genes can be effectively reduced.

In order to solve the above technical problem, the present invention provides a detection device based on an interdigitated electrode, including: the amplification cavity is used for gene amplification and is communicated with the first power source interface through a first channel; the detection cavity is communicated with the amplification cavity through a second channel, and a finger inserting electrode is arranged in the detection cavity; the flushing liquid cavity is used for containing flushing liquid, is communicated with the detection cavity through a third channel and is communicated with the second power source interface; the air blowing interface is communicated with the detection cavity through a fourth channel; wherein the sample in the amplification cavity is suitable for being conveyed into the detection cavity by an external power source so as to be hybridized with the biological probe on the finger-inserted electrode; the flushing liquid in the flushing liquid cavity is suitable for being externally driven

The force source is conveyed into the detection cavity to wash the hybridized finger inserting electrode; the blowing interface is suitable for an external air source to blow dry the flushed finger-inserted electrode through a fourth channel; the flushing liquid in the flushing liquid cavity is suitable for being conveyed to the detection cavity after blow-drying by an external power source, so that the finger inserting electrodes can be electrically detected.

Furthermore, the lower side of the detection cavity is provided with an electrode chip accommodating cavity for accommodating an electrode chip, and the finger inserting electrode area of the electrode chip is the cavity bottom of the detection cavity.

Further, the finger electrode-based detection device further comprises: the collection cavity is communicated with the detection cavity through a fifth channel and is communicated with an external collection interface; and the first channel, the second channel, the third channel, the fourth channel and the fifth channel are all provided with film valves.

Furthermore, the lower sides of the amplification cavity and the electrode chip accommodating cavity are respectively provided with a first heating cavity for heating the amplification cavity and the electrode chip accommodating cavity; one side of each first heating cavity is provided with a corresponding second heating cavity; one side of each second heating cavity is provided with a temperature measuring cavity; heating modules are arranged in the first heating cavity and the second heating cavity; and a temperature sensor is arranged in the temperature measuring cavity and used for detecting the temperature in the corresponding second heating cavity.

Further, the detection device based on the interdigitated electrode comprises a top plate, a middle plate and a bottom plate; the amplification cavity, the first channel, the first power source interface, the second channel, the flushing liquid cavity, the third channel, the second power source interface, the blowing interface, the fourth channel, the collection cavity, the fifth channel, the external connection collection interface, the first heating cavity, the second heating cavity and the temperature measurement cavity are all arranged on the bottom plate; the membrane valve is arranged between the middle plate and the bottom plate; the detection cavity is arranged on the middle plate; and the top plate and the middle plate are provided with filling ports respectively communicated with the amplification cavity and the flushing liquid cavity and a connecting port for electrically connecting with the electrode chip.

Further, the collection cavity is rectangular; the third channel and the fourth channel are connected with the same side surface of the collecting cavity, namely the first side surface; the fifth channel is connected with the opposite surface of the first side surface, namely the second side surface; the second channel connects the third side between the first side and the second side.

Furthermore, the first power source interface, the second power source interface, the blowing interface and the external collecting interface are all arranged on the side face of the bottom plate.

In another aspect, the present invention also provides a working method of the above-mentioned finger-insertion-electrode-based detection device, including: amplifying the gene; hybridizing the amplified gene with a biological probe on a finger inserting electrode; washing and drying the hybridized finger inserting electrode; covering the dried finger-inserted electrodes with pure water; and carrying out electrical detection on the finger inserting electrodes.

In another aspect, the present invention further provides a processing method of the above-mentioned detection device based on interdigitated electrodes, including: processing a top plate, a middle plate and a bottom plate; placing a temperature sensor in a temperature measuring cavity, placing an electrode chip which is bound by a probe in advance in an electrode chip accommodating cavity, and placing a thin film sheet at the position of a thin film valve; combining the top plate, the middle plate and the bottom plate; and placing the heating module into the first heating cavity and the second heating cavity.

Further, the bonding method comprises ultrasonic bonding and pressure sensitive tape connection.

The detection device based on the finger-inserted electrode has the advantages that by arranging the amplification cavity, the detection cavity, the flushing liquid cavity and the air blowing interface, a sample in the amplification cavity is suitable for being conveyed into the detection cavity by an external power source so as to be hybridized with a biological probe on the finger-inserted electrode; the flushing liquid in the flushing liquid cavity is suitable for being conveyed into the detection cavity by an external power source so as to flush the hybridized finger inserting electrode; the blowing interface is suitable for an external air source to blow dry the flushed finger-inserted electrode through a fourth channel; the flushing liquid in the flushing liquid cavity is suitable for being conveyed into the detection cavity after being dried by an external power source, so that the finger inserting electrodes can be electrically detected; the steps required in the gene detection process of the finger-inserted electrode are integrated into a chip, so that the detection method can be operated outside a laboratory, and the pollution of external genes can be effectively reduced.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings. In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is an exploded view of a finger-insertion electrode-based detection device of the present invention;

FIG. 2 is a schematic view of a middle plate of the interdigitated electrode based detection apparatus of the present invention;

FIG. 3 is a schematic bottom plate of the interdigitated electrode based detection apparatus of the present invention;

fig. 4 is a perspective view of the bottom plate of the finger-inserted electrode-based detection device of the present invention.

In the figure:

the device comprises a top plate 1, a filling port 11, a connecting port 12, a middle plate 2, a detection cavity 21, a second channel 211, a membrane valve 22, a bottom plate 3, an amplification cavity 31, a first channel 311, a first power source interface 312, a flushing liquid cavity 32, a third channel 321, a second power source interface 322, an electrode chip accommodating cavity 33, a collecting cavity 34, a fifth channel 341, an external collecting interface 342, a blowing interface 351, a fourth channel 352, a first heating cavity 36, a second heating cavity 37, a temperature measuring cavity 38, an electrode chip 4, a heating module 5 and a temperature sensor 6.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

As shown in fig. 1 to 4, the present embodiment provides a detection apparatus based on an interdigitated electrode, including: an amplification chamber 31 for gene amplification, communicating with a first power source interface 312 through a first channel 311; the detection cavity 21 is communicated with the amplification cavity 31 through a second channel 211, and a finger inserting electrode is arranged in the detection cavity; a flushing liquid cavity 32 for containing flushing liquid, which is communicated with the detection cavity 21 through a third passage 321, and is communicated with the second power source interface 322; an air blowing interface 351, which is communicated with the detection cavity 21 through a fourth channel 352; wherein the sample in the amplification chamber 31 is suitable for being transported into the detection chamber 21 by an external power source so as to be hybridized with the biological probe on the finger-inserted electrode; the flushing liquid in the flushing liquid cavity 32 is suitable for being conveyed into the detection cavity 21 by an external power source so as to flush the hybridized finger electrodes; the blowing interface 351 is suitable for an external air source to blow dry the washed finger-inserted electrode through the fourth channel 352; the flushing liquid in the flushing liquid cavity 32 is suitable for being conveyed into the detection cavity 21 after being dried by an external power source, so that the finger insertion electrodes can be electrically detected conveniently.

In the embodiment, the detection device based on the interdigital electrode integrates the procedures required by the interdigital electrode to detect the target gene into a chip, so that the detection method can be operated outside a laboratory, and the pollution of external genes can be effectively reduced.

As shown in fig. 3 and 4, optionally, an electrode chip accommodating cavity 33 is disposed at a lower side of the detection cavity 21 for accommodating the electrode chip 4, and the finger electrode area of the electrode chip 4 is a cavity bottom of the detection cavity 21.

As shown in fig. 3 and 4, in this embodiment, in order to facilitate the recovery of waste liquid, the finger-inserted electrode-based detection device further includes: a collection cavity 34 communicated with the detection cavity 21 through a fifth channel 341 and communicated with an external collection interface 342; and the first channel 311, the second channel 211, the third channel 321, the fourth channel 352 and the fifth channel 341 are all provided with the thin film valves 22.

In this embodiment, taking the structure of the thin film valve 22 on the first channel 311 as an example, an alternative implementation may be: the first channel 311 is interrupted, the bottom surface of the elastic thin film sheet covers the interrupted position, the middle plate 2 is provided with a pressurizing cavity, and the top surface of the elastic thin film sheet covers the bottom of the pressurizing cavity; when the fluid in the first channel 311 flows in the forward direction (i.e. enters the detection chamber 21 from the amplification chamber 31), the fluid can be powered by the external power source, and the fluid can overcome the elasticity of the elastic membrane and the gas pressure in the pressurizing chamber, and push the elastic membrane away from the break to flow forward; the reaction liquid may generate heat in the detection chamber 21, and the fluid is going to flow in the reverse direction (i.e. going to enter the amplification chamber 31 from the detection chamber 21), but it is not enough to overcome the elasticity of the elastic membrane and the gas pressure in the pressurizing chamber, i.e. the elastic membrane cannot be pushed open, and the membrane valve 22 has a certain function of a one-way valve.

In some application scenarios, the amplification chamber 31 and the detection chamber 21 need to have a constant temperature function, in this embodiment, it is preferable that the lower sides of the amplification chamber 31 and the electrode chip accommodating chamber 33 are respectively provided with a first heating chamber 36 for heating the amplification chamber 31 and the electrode chip accommodating chamber 33; a corresponding second heating cavity 37 is arranged on one side of each first heating cavity 36; a temperature measuring cavity 38 is arranged on one side of each second heating cavity 37; wherein the first heating cavity 36 and the second heating cavity 37 are both provided with heating modules 5; and a temperature sensor 6 is arranged in the temperature measuring cavity 38 and is used for detecting the temperature in the corresponding second heating cavity 37.

In the present embodiment, taking the implementation of constant temperature control on the amplification chamber 31 as an example, the heating modules 5 in the first heating chamber 36 and the second heating chamber 37 can be controlled by the same heating circuit, that is, the first heating chamber 36 and the second heating chamber 37 can be implemented with the same temperature; the first heating cavity 36 is used for directly heating the amplification cavity 31, and the second heating cavity 37 is used for measuring the temperature of the temperature sensor 6, so as to indirectly acquire the temperature of the amplification cavity 31, and can be used for controlling the constant temperature of the amplification cavity 31 through, but not limited to, a PID algorithm.

As shown in fig. 1, in the present embodiment, as a preferred assembly manner of the finger-insertion-electrode-based detection device, the finger-insertion-electrode-based detection device may include a top plate 1, a middle plate 2, and a bottom plate 3; wherein the amplification cavity 31, the first channel 311, the first power source interface 312, the second channel 211, the flushing liquid cavity 32, the third channel 321, the second power source interface 322, the blowing interface 351, the fourth channel 352, the collection cavity 34, the fifth channel 341, the external connection collection interface 342, the first heating cavity 36, the second heating cavity 37 and the temperature measuring cavity 38 are all arranged on the bottom plate 3; the membrane valve 22 is arranged between the intermediate plate 2 and the bottom plate 3; the detection chamber 21 is arranged on the intermediate plate 2; the top plate 1 and the middle plate 2 are provided with a filling port 11 respectively communicated with the amplification chamber 31 and the washing solution chamber 32, and a connecting port 12 for electrically connecting with the electrode chip 4.

As shown in fig. 3, in the present embodiment, optionally, the collection chamber 34 is rectangular; the third channel 321 and the fourth channel 352 are connected to the same side, i.e. the first side, of the collecting cavity 34; the fifth passage 341 connects the opposite surface of the first side surface, i.e., the second side surface; the second channel 211 connects a third side between the first side and the second side.

In this embodiment, the flow direction of the third and

fourth passages

321, 352 is the same direction as the fifth passage 341, which facilitates the gas-liquid flow into the fifth passage 341 during the blowing and cleaning.

In this embodiment, optionally, the first power source interface 312, the second power source interface 322, the blowing interface 351, and the external collection interface 342 are all disposed on the side of the bottom plate 3, and may be, but not limited to, a threaded structure.

In this embodiment, the external power source may be, but is not limited to, a piezoelectric pump or a peristaltic pump.

In this embodiment, the finger-inserted electrode-based detection device may be a microfluidic chip.

Example 2

On the basis of embodiment 1, this embodiment provides an operating method of the detection apparatus based on the interdigitated electrode as described in embodiment 1, including: amplifying the gene; hybridizing the amplified gene with a biological probe on a finger inserting electrode; washing and drying the hybridized finger inserting electrode; covering the dried finger-inserted electrodes with pure water; and carrying out electrical detection on the finger inserting electrodes.

In this embodiment, optionally, when performing the detection, firstly, the sample can be injected into the amplification chamber 31 from the filling port 11, the heating module 5 below the amplification chamber 31 and the temperature measurement chamber 38 works simultaneously after sealing, and the temperature sensor 6 in the temperature measurement chamber 38 detects and feeds back the temperature. After a certain time the pump is operated to push the liquid in the amplification chamber 31 above the interdigitated electrodes, i.e. in the detection chamber 21. At this time, the heating modules 5 in the first heating cavity 36 and the second heating cavity 37 start to work and perform temperature feedback. After a certain period of reaction, the air pump works to push part of the pure water in the rinsing liquid chamber 32 to the upper part of the finger electrode to complete the cleaning, and the liquid in the detection chamber 21 will pass through the channel to enter the collection chamber 34. Then the air pump associated with the fourth channel 352 is turned on and high velocity air is pumped through, and the majority of the liquid will follow the channel into the collection chamber 34 and continue to blow until the surfaces of the interdigitated electrodes are dry. At this time, pure water may be continuously introduced and dried, and the washing may be repeated 1 time. Then, a certain amount of pure water (10-20 μ L) is pushed in to cover the whole detection area above the finger-inserted electrode. And then, starting to work by an external detection hardware circuit, introducing a certain electric signal to detect an impedance spectrum, and analyzing according to the result of the impedance spectrum to obtain whether the sample contains the target gene.

Example 3

On the basis of the embodiment 1 and the embodiment 2, the present embodiment provides a processing method of the detection device based on the interdigitated electrode, which includes: processing a top plate 1, a middle plate 2 and a bottom plate 3; the temperature sensor 6 is placed in the temperature measuring cavity 38, the electrode chip 4 which is pre-finished with probe binding is placed in the electrode chip accommodating cavity 33, and the thin film piece is placed at the position of the thin film valve 22; combining a processed top plate 1, a middle plate 2 and a bottom plate 3; the heating module 5 is placed in the first heating cavity 36 and the second heating cavity 37.

In this embodiment, optionally, the top plate 1, the middle plate 2 and the bottom plate 3 may be processed by a high-precision 3d printer or a numerical control machine; after finishing the processing, the two temperature sensors 6 can be adhered to the bottom of the corresponding temperature measurement cavity 38 through glue; the electrode chip 4 is previously bound with the probe and then placed in the electrode chip accommodating chamber 33. The bonding of the entire chip can then be initiated by placing a pre-processed latex film of appropriate dimensions at the corresponding valve locations. Conventional thermocompression bonding may damage the structure of the probe, so that ultrasonic bonding may be used to bond 3 layers of chips together, and at this time, a layer of double-sided tape may be attached in advance above the finger electrode chip to ensure the close bonding of the chip and the middle plate 2. Or the bonding between the chips can be realized by directly using a Pressure Sensitive Adhesive (PSA) which is cut into corresponding shapes by a cutting machine to ensure the connectivity of the channels and the valves. And after the bonding is finished, the heating module 5 is placed into the corresponding cavity, and the upper surfaces of the other cavities are attached by using glue, so that the heat is directly transferred to the chip. And then screwing the threaded connector on the chip, and communicating the threaded connector with an external pump through a hose, so that the test can be started.

In conclusion, the detection device based on the finger-inserted electrode is designed for the matched finger-inserted electrode chip, and finally realizes the detection of the target gene by using the finger-inserted electrode outside a laboratory; integrates the functions of temperature control, fluid valves, gene amplification, gene detection, chip washing and drying, waste liquid recovery and the like.

The components selected for use in the present application (components not illustrated for specific structures) are all common standard components or components known to those skilled in the art, and the structure and principle thereof can be known to those skilled in the art through technical manuals or through routine experimentation.

In the description of the embodiments of the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. A detection device based on finger insertion electrodes is characterized by comprising:

an amplification chamber (31) for gene amplification, communicating with a first power source interface (312) via a first channel (311);

the detection cavity (21) is communicated with the amplification cavity (31) through a second channel (211), and a finger inserting electrode is arranged in the detection cavity;

a flushing liquid cavity (32) used for containing flushing liquid, communicated with the detection cavity (21) through a third channel (321), and communicated with the second power source interface (322);

the blowing interface (351) is communicated with the detection cavity (21) through a fourth channel (352); wherein

The sample in the amplification cavity (31) is suitable for being conveyed into the detection cavity (21) by an external power source so as to be hybridized with the biological probe on the finger-inserted electrode;

the flushing liquid in the flushing liquid cavity (32) is suitable for being conveyed into the detection cavity (21) by an external power source so as to flush the hybridized finger electrodes;

the blowing interface (351) is suitable for an external air source to blow dry the flushed finger-inserted electrode through a fourth channel (352);

the flushing liquid in the flushing liquid cavity (32) is suitable for being conveyed into the detection cavity (21) after being dried by an external power source, so that the finger inserting electrodes can be electrically detected conveniently.

2. The interdigitated electrode-based detection apparatus of claim 1,

an electrode chip accommodating cavity (33) is formed in the lower side of the detection cavity (21) and used for placing the electrode chip (4), and the finger inserting electrode area of the electrode chip (4) is the cavity bottom of the detection cavity (21).

3. The interdigitated electrode-based detection apparatus of claim 2, further comprising:

the collection cavity (34) is communicated with the detection cavity (21) through a fifth channel (341) and is communicated with an external collection interface (342); and

the first channel (311), the second channel (211), the third channel (321), the fourth channel (352) and the fifth channel (341) are all provided with a thin film valve (22).

4. The interdigitated electrode-based detection apparatus of claim 3,

the lower sides of the amplification cavity (31) and the electrode chip accommodating cavity (33) are respectively provided with a first heating cavity (36) for heating the amplification cavity (31) and the electrode chip accommodating cavity (33);

one side of each first heating cavity (36) is provided with a corresponding second heating cavity (37);

a temperature measuring cavity (38) is arranged at one side of each second heating cavity (37); wherein

Heating modules (5) are arranged in the first heating cavity (36) and the second heating cavity (37); and

and a temperature sensor (6) is arranged in the temperature measuring cavity (38) and is used for detecting the temperature in the corresponding second heating cavity (37).

5. The interdigitated electrode-based detection apparatus of claim 4,

the detection device based on the interdigital electrode comprises a top plate (1), a middle plate (2) and a bottom plate (3); wherein

The amplification cavity (31), the first channel (311), the first power source interface (312), the second channel (211), the flushing liquid cavity (32), the third channel (321), the second power source interface (322), the blowing interface (351), the fourth channel (352), the collection cavity (34), the fifth channel (341), the external connection collection interface (342), the first heating cavity (36), the second heating cavity (37) and the temperature measurement cavity (38) are all arranged on the bottom plate (3);

the membrane valve (22) is arranged between the middle plate (2) and the bottom plate (3);

the detection cavity (21) is arranged on the middle plate (2);

the top plate (1) and the middle plate (2) are provided with filling ports (11) which are respectively communicated with the amplification cavity (31) and the flushing liquid cavity (32), and connecting ports (12) which are used for being electrically connected with the electrode chip (4).

6. The interdigitated electrode-based detection apparatus of claim 5,

the collection chamber (34) is rectangular;

the third channel (321) and the fourth channel (352) are connected with the same side of the collecting cavity (34), namely the first side;

the fifth channel (341) is connected with the opposite surface of the first side surface, namely the second side surface;

the second channel (211) connects a third side between the first side and the second side.

7. The interdigitated electrode-based detection apparatus of claim 5,

the first power source interface (312), the second power source interface (322), the blowing interface (351) and the external collecting interface (342) are all arranged on the side face of the bottom plate (3).

8. A method of operating an interdigitated electrode based detection apparatus as claimed in any one of claims 1 to 7, comprising:

amplifying the gene;

hybridizing the amplified gene with a biological probe on a finger inserting electrode;

washing and drying the hybridized finger inserting electrode;

covering the dried finger-inserted electrodes with pure water;

and carrying out electrical detection on the finger inserting electrodes.

9. A method of manufacturing a fingered-based detection apparatus as claimed in any one of claims 1-7, comprising:

processing a top plate (1), a middle plate (2) and a bottom plate (3);

placing a temperature sensor (6) in a temperature measuring cavity (38), placing an electrode chip (4) which is pre-bound by a probe in an electrode chip accommodating cavity (33), and placing a film piece at the position of a film valve (22);

combining a top plate (1), a middle plate (2) and a bottom plate (3);

the heating module (5) is placed in the first heating cavity (36) and the second heating cavity (37).

10. The method for manufacturing a finger-inserted electrode-based detection device according to claim 9,

the bonding method comprises ultrasonic bonding and pressure sensitive tape connection.