CN115282946A - Simulant for testing virus purification performance in water purification device and preparation method thereof - Google Patents
Simulant for testing virus purification performance in water purification device and preparation method thereof Download PDFInfo
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- CN115282946A CN115282946A CN202210959356.2A CN202210959356A CN115282946A CN 115282946 A CN115282946 A CN 115282946A CN 202210959356 A CN202210959356 A CN 202210959356A CN 115282946 A CN115282946 A CN 115282946A
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- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
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- B01J20/28021—Hollow particles, e.g. hollow spheres, microspheres or cenospheres
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
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Abstract
The invention belongs to the field of new material detection and metering standardization, and particularly relates to a simulant for testing virus purification performance in a water purification device and a preparation method thereof. The mimetic was prepared by the following method: (1) Adding a certain amount of n-butyl titanate into a water-ethanol solution of hexadecyl trimethyl ammonium bromide, and stirring to obtain pretreated titanium dioxide; (2) And adding sodium alginate into the pretreated titanium dioxide, uniformly stirring, adding the cross-linking agent again, continuously stirring for reaction, centrifugally separating after the reaction is finished, washing and drying to obtain the titanium dioxide @ sodium alginate microspheres. The virus simulant prepared by the invention has simple preparation method and lower raw material cost. The particle size of the titanium dioxide @ sodium alginate microspheres prepared by the method is similar to that of viruses, the surfaces of the titanium dioxide @ sodium alginate microspheres have active groups similar to those of the viruses, the titanium dioxide @ sodium alginate microspheres are nontoxic, the experimental safety requirement is low, the particle size is controllable, and the titanium dioxide @ sodium alginate microspheres can simulate the viruses to perform related performance tests on a water purifying device.
Description
Technical Field
The invention belongs to the field of new material detection and metering standardization, and particularly relates to a simulant for testing virus purification performance in a water purification device and a preparation method thereof.
Background
Bacteriophages are a generic term for viruses that infect microorganisms such as bacteria, fungi, actinomycetes, and spirochetes, and are called bacteriophages because they are partially responsible for the lysis of the host bacteria. The phage mainly comprises two categories, namely double-stranded phage and single-stranded filamentous phage, wherein the double-stranded phage is lambda-type phage, and the single-stranded filamentous phage comprises M13, f1 and fd phage. At present, the adoption of phage as a simulated virus has become a research hotspot in the fields of evaluating the treatment effect of water and sewage, clarifying a virus inactivation mechanism, modifying a virus detection method and the like.
However, since bacteriophage is used as a mimic virus, the controllability of particle size is poor, and the cultivation process is complicated. Therefore, the development of a novel virus-mimic material for mimicking the method of phage removal is an urgent problem to be solved.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a simulant for testing the virus purification performance in a water purification device and a preparation method thereof.
The technical scheme adopted by the invention for realizing the purpose is as follows:
the invention provides a preparation method of a simulant for testing virus purification performance in a water purification device, which comprises the following steps:
(1) Adding a certain amount of n-butyl titanate into a water-ethanol solution of hexadecyl trimethyl ammonium bromide, and stirring to obtain pretreated titanium dioxide;
(2) And adding sodium alginate into the pretreated titanium dioxide, uniformly stirring, adding the cross-linking agent again, continuously stirring for reaction, centrifugally separating after the reaction is finished, washing and drying to obtain the titanium dioxide @ sodium alginate microspheres.
Further, in the step (1), the mass ratio of the water-ethanol solution of the n-butyl titanate and the hexadecyl trimethyl ammonium bromide is 1; the mass concentration of the water-ethanol solution of the hexadecyl trimethyl ammonium bromide is 20%; the volume ratio of the water to the ethanol is 5.
Further, in the step (1), the stirring time is 40-50min.
Further, in the step (2), the mass ratio of the titanium dioxide to the sodium alginate is 2-3; the mass ratio of the cross-linking agent to the sodium alginate is 0.08-0.1.
Further, the cross-linking agent is composed of triethyl formate and tyrosine according to a mass ratio of 1.
Further, in the step (2), the stirring reaction time is 1-2h.
The invention provides titanium dioxide @ sodium alginate microspheres prepared by the preparation method of any one of claims 1-6.
The beneficial effects of the invention are as follows:
(1) The virus simulant prepared by the invention has simple preparation method and lower raw material cost.
(2) The particle size of the titanium dioxide @ sodium alginate microspheres prepared by the method is similar to that of viruses, the surfaces of the titanium dioxide @ sodium alginate microspheres have active groups similar to those of the viruses, the titanium dioxide @ sodium alginate microspheres are nontoxic, the experimental safety requirement is low, the particle size is controllable, and the titanium dioxide @ sodium alginate microspheres can simulate the viruses to perform related performance tests on a water purifying device.
Drawings
FIG. 1 is a scanning electron microscope image of the titanium dioxide @ sodium alginate microspheres prepared in example 1.
Detailed Description
The technical solution of the present invention is further explained and illustrated by the following specific examples.
Example 1 (1) n-butyl titanate was added to a water-ethanol solution (mass concentration of 20%) of cetyltrimethylammonium bromide in a mass ratio of 1;
(2) Adding sodium alginate into the pretreated titanium dioxide according to the mass ratio of 2.
The scanning electron microscope image of the prepared titanium dioxide @ sodium alginate microsphere is shown in figure 1.
Example 2
(1) Adding a water-ethanol solution (mass concentration is 20%) of hexadecyl trimethyl ammonium bromide into n-butyl titanate according to a mass ratio of 1.2, and stirring for 40min to obtain pretreated titanium dioxide;
(2) Adding sodium alginate into the pretreated titanium dioxide according to the mass ratio of 3.
Comparative example 1
(1) Adding a water-ethanol solution (with the mass concentration of 20%) of hexadecyl trimethyl ammonium bromide into n-butyl titanate according to a mass ratio of 1;
(2) Adding sodium alginate into the pretreated titanium dioxide according to the mass ratio of 2.
Comparative example 2
(1) Adding a water-ethanol solution (with the mass concentration of 20%) of hexadecyl trimethyl ammonium bromide into n-butyl titanate according to a mass ratio of 1;
(2) Adding sodium alginate into the pretreated titanium dioxide according to the mass ratio of 2 to 1, uniformly stirring, adding tyrosine accounting for 8 percent of the mass of the sodium alginate again, continuously stirring for reacting for 2 hours, after the reaction is finished, performing centrifugal separation, washing and drying to obtain the titanium dioxide @ sodium alginate microspheres.
It is noted that in comparative examples 1 and 2, microspheres were prepared by adding a single crosslinking agent, and a part of the surface of the titanium dioxide spheres was not coated with sodium alginate.
Effects of the embodiment
The titanium dioxide @ sodium alginate microspheres prepared in the examples and the comparative examples are used as a simulant for simulating the removal efficiency of viruses in a water purifying device, and phage is used as a control group, wherein the specific requirements are as follows:
1. test environment and operating requirements
The test adopts aseptic operation technology, and the laboratory environment, test operation and other parts related to biological safety meet the requirements of GB 19489.
Control phage and host
a) Phage display: phi-X174 (ATCC 13706-B1, NBRC 103405)
Host bacteria: escherichia coli (ATCC 13706, NBRC 13898)
b) Phage display: MS2 (NBRC 102619)
Host bacteria: escherichia coli (Escherichia coli) (NBRC 3012)
3. Preparation of phage suspension
a) Inoculating host bacterium Escherichia coli on nutrient agar medium (NA) plate, culturing at 37 + -1 deg.C for 24 + -1 h, inoculating single colony in nutrient broth medium, and oscillating at 35 + -1 deg.C for 5 + -1 h at 100 r/min;
b) Pouring 60 mL of nutrient agar into a culture dish, and solidifying for later use;
c) 5 mL of 10 8 CFU/mL~10 9 CFU/mL suspension of Escherichia coli with 5 mL of 10 concentration 5 PFU/mL～10 6 Phage suspension mixing of PFU/mL (according to Escherichia coli: phage = 1)000: 1) is kept stand for 10min to 20min at the temperature of (35 +/-1) ° c;
d) Adding 10 mL of semisolid culture medium into the mixed solution obtained in the step c), uniformly mixing, pouring onto the nutrient agar solid culture medium prepared in the step b), and standing for culture (18 +/-2) h without inversion at the temperature of (35 +/-1) ° C. After culturing, recovering the upper semi-solid culture medium into a sterile bag by using a sterile coating rod, rotating at 260 r/min for 2 min, and standing at the temperature of (35 +/-1) ° C;
e) Transferring the liquid into a 50 mL centrifuge tube, centrifuging for 10min at 3500 r/min, transferring the supernatant into another 50 mL centrifuge tube, centrifuging under the same conditions, and repeating for 2 times;
f) The supernatant was filtered through a filter having a pore size of 0.22. Mu.m, to obtain a phage stock solution for testing.
Test procedure
a) In the examples and comparative example groups: adding the prepared titanium dioxide @ sodium alginate microspheres into water to be detected; in the control group: diluting the phage stock solution with sterilized deionized water to adjust the concentration of phage stock solution to 10 6 PFU/mL～10 7 PFU/mL；
b) The test is carried out under the condition that the water inlet pressure is (0.24 +/-0.02) MPa. When the water purification device reaches 0%, 25%, 50%, 75% and 100% of the nominal rated total water purification amount, sampling is respectively carried out at an inflow sample sampling point and an outflow sample sampling point. The sampling amount should not exceed 1L or 4 times of the amount required for analysis, whichever is larger. The collection, storage and test of samples are carried out according to the method specified in GB/T5750 (all parts), and the test is carried out according to other related standards at home and abroad which are not specified in GB/T5750. c) After sampling, in the examples and comparative examples, the samples after passing through a water purifying device are centrifuged, and the removal rate is detected; phage titers were determined according to method 5.
Determination of phage titer
The recovered phage suspension was used as a reagent stock solution, and diluted 10-fold with sterile deionized water or PBS. According to Escherichia coli: phage =1000:1, mixing with a suspension of Escherichia coli at a corresponding concentration, and standing at (35 +/-1) deg.C for 10-20 min. Mixing the bacterial suspension after standing with a semi-solid agar culture medium, pouring the mixture on the surface of the solid agar culture medium, and culturing for 16-24 h without inversion at the temperature of (35 +/-1) ° C. After incubation, the number of phages in the test and control groups was counted.
Data processing
The phage removal rate was calculated according to equations (1) and (2):
in the formula:
the removal rate of the simulants in the examples and comparative examples was counted, and the specific results are shown in table 1.
TABLE 1
And secondly, diluting the titanium dioxide @ sodium alginate microspheres prepared in the embodiment 1 and the comparative examples 1-2 by using deionized water to prepare suspension liquid, wherein the concentrations of all experimental groups are the same, and the turbidity values of the suspension liquid are respectively detected at 0min, 30min, 60min, 90min and 120min, and the specific results are shown in table 2.
TABLE 2
As can be seen from Table 2, the simulant prepared by the invention has good standing stability and can be uniformly dispersed in a system to be detected.
Claims (7)
1. A preparation method of a simulant for testing virus purification performance in a water purification device is characterized by comprising the following steps:
(1) Adding a certain amount of n-butyl titanate into a water-ethanol solution of hexadecyl trimethyl ammonium bromide, and stirring to obtain pretreated titanium dioxide;
(2) And adding sodium alginate into the pretreated titanium dioxide, uniformly stirring, adding the cross-linking agent again, continuously stirring for reaction, centrifugally separating after the reaction is finished, washing and drying to obtain the titanium dioxide @ sodium alginate microspheres.
2. The production method according to claim 1, wherein in the step (1), the mass ratio of the water-ethanol solution of n-butyl titanate and cetyltrimethylammonium bromide is 1; the mass concentration of the water-ethanol solution of the hexadecyl trimethyl ammonium bromide is 20 percent; the volume ratio of the water to the ethanol is 5.
3. The method according to claim 2, wherein in the step (1), the stirring time is 40 to 50min.
4. The preparation method according to claim 1, wherein in the step (2), the mass ratio of the titanium dioxide to the sodium alginate is 2-3; the mass ratio of the cross-linking agent to the sodium alginate is 0.08-0.1.
5. The preparation method according to claim 4, characterized in that the crosslinking agent consists of triethyl formate and tyrosine in a mass ratio of 1.
6. The method according to claim 1 or 4, wherein in the step (2), the stirring reaction time is 1-2h.
7. Titanium dioxide @ sodium alginate microspheres prepared by the preparation method of any one of claims 1-6.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210959356.2A CN115282946A (en) | 2022-08-11 | 2022-08-11 | Simulant for testing virus purification performance in water purification device and preparation method thereof |
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| CN202210959356.2A CN115282946A (en) | 2022-08-11 | 2022-08-11 | Simulant for testing virus purification performance in water purification device and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579643A (en) * | 2009-06-11 | 2009-11-18 | 湖州师范学院 | Sol-gel in situ preparation method of titanium dioxide nano powder coated with alginate |
| CN103170373A (en) * | 2013-04-01 | 2013-06-26 | 湖南大学 | Photocatalytic composite gel bead and preparation method as well as application thereof |
| CN106391132A (en) * | 2016-08-30 | 2017-02-15 | 上海交通大学 | Preparation method of hydrogel immobilized TiO2 photocatalyst |
| CN113441182A (en) * | 2021-06-29 | 2021-09-28 | 安徽农业大学 | Preparation method of core-shell titanium dioxide @ carboxyl chitosan nanoparticles |
| RO135381A2 (en) * | 2020-05-29 | 2021-12-30 | Universitatea Politehnica Din Bucureşti | Composition and process for making micro spheres based on alginate and titanium oxide (tio2) nanoparticles, with application in environment protection |
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2022
- 2022-08-11 CN CN202210959356.2A patent/CN115282946A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579643A (en) * | 2009-06-11 | 2009-11-18 | 湖州师范学院 | Sol-gel in situ preparation method of titanium dioxide nano powder coated with alginate |
| CN103170373A (en) * | 2013-04-01 | 2013-06-26 | 湖南大学 | Photocatalytic composite gel bead and preparation method as well as application thereof |
| CN106391132A (en) * | 2016-08-30 | 2017-02-15 | 上海交通大学 | Preparation method of hydrogel immobilized TiO2 photocatalyst |
| RO135381A2 (en) * | 2020-05-29 | 2021-12-30 | Universitatea Politehnica Din Bucureşti | Composition and process for making micro spheres based on alginate and titanium oxide (tio2) nanoparticles, with application in environment protection |
| CN113441182A (en) * | 2021-06-29 | 2021-09-28 | 安徽农业大学 | Preparation method of core-shell titanium dioxide @ carboxyl chitosan nanoparticles |
Non-Patent Citations (1)
| Title |
|---|
| 张艳青等: "模板法制备介孔TiO2及其在模拟太阳光下光催化还原Cr（VI）性能的研究" * |
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