CN115266597A - Optical imaging method, device and system - Google Patents
Optical imaging method, device and system Download PDFInfo
- Publication number
- CN115266597A CN115266597A CN202210721653.3A CN202210721653A CN115266597A CN 115266597 A CN115266597 A CN 115266597A CN 202210721653 A CN202210721653 A CN 202210721653A CN 115266597 A CN115266597 A CN 115266597A
- Authority
- CN
- China
- Prior art keywords
- light
- sample
- signal
- detection
- pulse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 65
- 238000012634 optical imaging Methods 0.000 title claims abstract description 50
- 238000001514 detection method Methods 0.000 claims abstract description 123
- 238000003384 imaging method Methods 0.000 claims abstract description 88
- 239000000523 sample Substances 0.000 claims description 167
- 230000003287 optical effect Effects 0.000 claims description 59
- 238000001914 filtration Methods 0.000 claims description 51
- 238000012545 processing Methods 0.000 claims description 29
- 230000008569 process Effects 0.000 claims description 17
- 230000010287 polarization Effects 0.000 claims description 13
- 230000001678 irradiating effect Effects 0.000 claims description 10
- 230000000630 rising effect Effects 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 20
- 238000004590 computer program Methods 0.000 description 15
- 238000005516 engineering process Methods 0.000 description 8
- 238000004891 communication Methods 0.000 description 6
- 238000010276 construction Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000001931 thermography Methods 0.000 description 3
- 238000013500 data storage Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000004476 mid-IR spectroscopy Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 210000004895 subcellular structure Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/171—Systems in which incident light is modified in accordance with the properties of the material investigated with calorimetric detection, e.g. with thermal lens detection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
- G01J5/48—Thermography; Techniques using wholly visual means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/171—Systems in which incident light is modified in accordance with the properties of the material investigated with calorimetric detection, e.g. with thermal lens detection
- G01N2021/1714—Photothermal radiometry with measurement of emission
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Abstract
The present disclosure relates to the technical field of optical imaging, and in particular, to an optical imaging method, apparatus, and system, wherein the method comprises: controlling detection light and mid-infrared pump light to irradiate a sample, wherein the detection light is continuous light or pulse light, and the mid-infrared pump light is mid-infrared pulse light with a set wavelength; acquiring a photoelectric signal of emergent light of the sample irradiated by the detection light; acquiring a photo-thermal signal of the emergent light according to the photoelectric signal, and acquiring unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal; and controlling an irradiation light path to scan the sample to obtain a plurality of unit area imaging data until the target area imaging data of the sample is obtained according to the unit area imaging data. The method realizes high-precision imaging of the sample, and meets the requirements of different imaging precisions.
Description
Technical Field
The present disclosure relates to the field of optical imaging technologies, and in particular, to an optical imaging method, apparatus, and system.
Background
In the field of microscopy imaging, how to achieve label-free imaging with high sensitivity has been a hot issue. The need for such highly sensitive label-free imaging methods is particularly evident in metabolic and molecular biology research, due to the need to track and analyze the movement, transport, metabolic processes of specific molecules, specific substances or specific subcellular structures in dynamic, living cells. The photothermal microscopic imaging technology is a technology which utilizes the property of a specific molecular bond and photothermal effect, irradiates a sample with light with specific wavelength, enables the corresponding part of the sample to generate thermal effect, thereby causing the optical property to change, and is captured, amplified and analyzed by an optical means, thereby completing the imaging of a specific structure or a substance, does not need to use molecular markers, can observe a dynamic sample, and has obvious advantages.
In the conventional technology, when imaging identification is carried out by adopting a photothermal microscopic imaging technology, a phase-locked amplifier is often required to collect light intensity signals, the energy of photothermal signals is easily lost, and the sensitivity of optical imaging is limited.
Disclosure of Invention
In view of the foregoing, it is desirable to provide an optical imaging method, apparatus, system, computer device, storage medium and computer program product for addressing the above technical problems.
In a first aspect, the present disclosure provides a method of optical imaging. The method comprises the following steps:
controlling detection light and mid-infrared pump light to irradiate a sample, wherein the detection light is continuous light or pulse light, and the mid-infrared pump light is mid-infrared pulse light with a set wavelength;
acquiring a photoelectric signal of emergent light of the sample irradiated by the detection light;
acquiring a photo-thermal signal of the emergent light according to the photoelectric signal, and acquiring unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal;
and controlling an irradiation light path to scan the sample to obtain a plurality of unit area imaging data until the target area imaging data of the sample is obtained according to the unit area imaging data.
In one embodiment, the emergent light is reflected light or transmitted light of the sample irradiated by the detection light.
In one embodiment, in a case where the probe light is continuous light, the obtaining a photothermal signal of the outgoing light from the photoelectric signal, and obtaining imaging data per unit area of the sample irradiated with the probe light from the photothermal signal includes:
carrying out first filtering processing on the photoelectric signal to obtain a first filtering signal, and extracting a harmonic component of the first filtering signal;
carrying out second filtering processing on the harmonic component to obtain a second filtering signal;
obtaining the photothermal signal from the second filtered signal;
and acquiring unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal.
In one embodiment, the second filtering process includes narrowband filtering the harmonic component.
In one embodiment, in the case that the detection light is pulsed light, the detection light includes a first pulse and a second pulse within a single pulse period, the first pulse is set before a rising edge of the mid-infrared pump light for a set period of time, and the second pulse is set after a falling edge of the mid-infrared pump light for a set period of time;
the acquiring of the photoelectric signal of the emergent light after the sample is irradiated by the probe light comprises:
and acquiring a first photoelectric signal of the first pulse, and acquiring a second photoelectric signal of the second pulse.
In a second aspect, the present disclosure also provides an optical imaging apparatus. The device comprises: the light source module is used for controlling the irradiation of detection light and mid-infrared pump light on the sample, wherein the detection light is continuous light or pulse light, and the mid-infrared pump light is mid-infrared pulse light with a set wavelength;
the optical detection module is used for collecting a photoelectric signal of emergent light after the sample is irradiated by the probe light;
the data processing module is used for obtaining a photo-thermal signal of the emergent light according to the photoelectric signal and obtaining unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal;
and the scanning module is used for controlling the irradiation light path to scan the sample to obtain a plurality of unit area imaging data until the target area imaging data of the sample is obtained according to the unit area imaging data.
In one embodiment, the emergent light is reflected light or transmitted light of the sample irradiated by the detection light.
In one embodiment, in the case that the detection light is a continuous light, the data processing module includes:
the first filtering unit is used for carrying out first filtering processing on the photoelectric signal to obtain a first filtering signal and extracting a harmonic component of the first filtering signal;
the second filtering unit is used for carrying out second filtering processing on the harmonic component to obtain a second filtering signal;
a photothermal signal unit for obtaining the photothermal signal from the second filtered signal;
and the imaging unit is used for obtaining the imaging data of the unit area of the sample irradiated by the detection light according to the photothermal signal.
In one embodiment, the second filtering process includes narrowband filtering the harmonic component.
In one embodiment, in the case that the detection light is pulsed light, the detection light includes a first pulse and a second pulse within a single pulse period, the first pulse is set before a rising edge of the mid-infrared pump light for a set period of time, and the second pulse is set after a falling edge of the mid-infrared pump light for a set period of time;
the optical detection module comprises a pulse unit for acquiring a first photoelectric signal of the first pulse and acquiring a second photoelectric signal of the second pulse.
In a third aspect, the present disclosure also provides an optical imaging system. The system comprises:
the intermediate infrared pump light source is used for emitting intermediate infrared pulse light with set wavelength to irradiate the sample;
the detection light source is used for emitting detection light to irradiate a sample, and the detection light is continuous light or pulse light;
the photoelectric detection device is used for collecting a photoelectric signal of the detection light;
the data acquisition device is used for acquiring a photoelectric signal output by the photoelectric detection device and acquiring a photo-thermal signal of the emergent light according to the photoelectric signal;
the upper computer, with well infrared pump light source, detection light source, photoelectric detection device, data acquisition device electric connection, the upper computer is used for according to the light and heat signal of data acquisition device output carries out the formation of image.
In one embodiment, the system further comprises an optical path module, in the case that the emergent light is reflected light of the sample irradiated by the detection light, the optical path module comprises an objective lens, a first optical path component, a beam splitter, a first scanning galvanometer, and the detection light reaches the objective lens through the beam splitter, the first scanning galvanometer, the first optical path component and irradiates the sample through the objective lens; the reflected light of the sample irradiated by the detection light passes through the objective lens, the first light path component, the first scanning galvanometer and the spectroscope to reach the photoelectric detection device.
In one embodiment, the system further comprises an optical path module, in the case that the emergent light is transmitted light of the sample irradiated by the detection light, the optical path module comprises a first scanning galvanometer, a second optical path component, an objective lens and a spectroscope, the detection light reaches the objective lens through the first scanning galvanometer and the second optical path component, and the sample is irradiated by the objective lens; the transmitted light of the sample irradiated by the probe light reaches the photoelectric detection device through the spectroscope.
In one embodiment, the system further comprises a light path module, wherein in the case that the emergent light is transmitted light of the sample irradiated by the detection light, the light path module comprises a first scanning galvanometer, a third light path component, an objective lens and a spectroscope, and the detection light irradiates the sample through the first scanning galvanometer and the spectroscope; the transmitted light of the sample irradiated by the detection light reaches the photoelectric detection device through the objective lens and the third light path component.
In one embodiment, the optical path module further includes a second scanning galvanometer, and the second scanning galvanometer is disposed between the mid-infrared pump light source and the sample.
In one embodiment, in the case that the detection light is pulsed light, the detection light includes pulsed light of two polarization components; the emergent light reaches the photoelectric detection device through the polarization light splitting component.
In a fourth aspect, the present disclosure also provides a computer device. The computer device comprises a memory storing a computer program and a processor implementing the steps of the above-described optical imaging method when executing the computer program.
In a fifth aspect, the present disclosure also provides a computer-readable storage medium. The computer-readable storage medium has stored thereon a computer program which, when being executed by a processor, carries out the steps of the above-mentioned optical imaging method.
In a sixth aspect, the present disclosure also provides a computer program product. The computer program product comprises a computer program which, when being executed by a processor, carries out the steps of the above-mentioned optical imaging method.
The optical imaging method, the optical imaging device, the optical imaging system, the optical imaging computer device, the optical imaging storage medium and the optical imaging computer program product at least have the following beneficial effects:
according to the imaging device, a sample is irradiated by intermediate infrared pulse light to generate a thermal effect, emergent light of the sample irradiated by detection light is obtained, and two-dimensional imaging of the sample is performed according to a photo-thermal signal of the emergent light, so that high-precision imaging of the sample is realized; the detection light supports continuous light and pulse light, meets the imaging requirements of different occasions and meets the requirements of different imaging accuracies; meanwhile, the irradiation light path of the detection light is controlled, and the sample is scanned to realize two-dimensional imaging of the target area of the sample.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments or the conventional technologies of the present disclosure, the drawings used in the descriptions of the embodiments or the conventional technologies will be briefly introduced below, it is obvious that the drawings in the following descriptions are only some embodiments of the present disclosure, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a diagram of an exemplary optical imaging method;
FIG. 2 is a schematic flow chart diagram illustrating a method for optical imaging in one embodiment;
FIG. 3 is a schematic view of an irradiation optical path scanning of the probe light in one embodiment;
FIG. 4 is a schematic view of an irradiation optical path scanning of the probe light in one embodiment;
FIG. 5 is a schematic view of an irradiation optical path scanning of the probe light in one embodiment;
FIG. 6 is a schematic flow chart diagram illustrating a method for optical imaging in one embodiment;
FIG. 7 is a diagram illustrating the processing of an optical signal according to one embodiment;
FIG. 8 is a diagram illustrating the processing of an optoelectronic signal in one embodiment;
FIG. 9 is a block diagram showing the structure of an optical imaging apparatus according to an embodiment;
FIG. 10 is a block diagram showing the structure of an optical imaging apparatus according to an embodiment;
FIG. 11 is a block diagram showing the construction of an optical imaging system according to an embodiment;
FIG. 12 is a block diagram showing the construction of an optical imaging system in one embodiment;
FIG. 13 is a block diagram showing the construction of an optical imaging system in one embodiment;
FIG. 14 is a block diagram showing the construction of an optical imaging system in one embodiment;
FIG. 15 is a block diagram showing the construction of an optical imaging system according to an embodiment;
FIG. 16 is a block diagram showing the construction of an optical imaging system in one embodiment;
FIG. 17 is a block diagram showing a configuration of an optical imaging system in one embodiment;
FIG. 18 is a block diagram of a polarization beam splitter assembly in one embodiment;
FIG. 19 is a block diagram showing an internal configuration of a computer device according to an embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more clearly understood, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. The terminology used herein in the description of the disclosure is for the purpose of describing particular embodiments only and is not intended to be limiting of the disclosure.
It should be noted that the terms "first," "second," and the like in the description and claims of the present disclosure and in the foregoing drawings are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the disclosure described herein are capable of operation in other sequences than those illustrated or described herein. The implementations described in the exemplary embodiments below are not intended to represent all implementations consistent with the present disclosure. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present disclosure, as detailed in the appended claims. The terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, the presence of additional identical or equivalent elements in a process, method, article, or apparatus that comprises the recited elements is not excluded. For example, if the terms first, second, etc. are used to denote names, they do not denote any particular order.
It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or be connected to the other element through intervening elements. In addition, "connection" in the following embodiments is understood to mean "electrical connection", "communication connection", and the like if there is a transfer of electrical signals or data between the connected objects.
As used herein, the singular forms "a", "an" and "the" may include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises/comprising," "includes" or "including," etc., specify the presence of stated features, integers, steps, operations, components, parts, or combinations thereof, but do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof. Also, in this specification, the term "and/or" includes any and all combinations of the associated listed items.
The optical imaging method provided by the embodiment of the application can be applied to the application environment as shown in fig. 1. Wherein, the terminal 102 is equipped with a display device, the terminal 102 is connected with the data acquisition device 104, the terminal 102 and the data acquisition device 104 may belong to different hardware devices, or the data acquisition device 104 may be integrated on the terminal 102 as a part of the terminal 102. The terminal 102 is configured to receive a data signal (for example, a photothermal signal) transmitted by the data acquisition device 104, perform imaging processing on the received photothermal signal based on a thermal imaging principle, and display imaging information through a display device. The data storage system may store data that the terminal 102 needs to process. The data storage system may be integrated on the terminal 102, or may be placed on the cloud or other network server. The terminal 102 may be, but is not limited to, various personal computers, notebook computers, smart phones, tablet computers, and the like.
In some embodiments of the present disclosure, as shown in fig. 2, an optical imaging method is provided, which is described by taking the method as an example for being applied to the terminal in fig. 1, and includes the following steps:
step S10: the method comprises the steps of controlling detection light and mid-infrared pump light to irradiate a sample, wherein the detection light is continuous light or pulse light, and the mid-infrared pump light is mid-infrared pulse light with a set wavelength.
Specifically, the terminal can control the mid-infrared pump light source to emit mid-infrared pulse light with a set wavelength, and control the detection light source to emit continuous light or pulse light. The intermediate infrared pump light source can directly irradiate the sample, and the detection light can directly irradiate the sample or indirectly irradiate the sample through the optical component. The mid-infrared pump light source irradiates a sample, and generally interacts with specific components in the sample to generate a thermal effect and change the optical property of the sample, so that physical quantities such as the intensity of a probe light emergent beam, the beam directivity and the like are influenced.
Step S20: and acquiring a photoelectric signal of emergent light of the sample irradiated by the detection light.
Specifically, when the probe light irradiates the sample directly or indirectly through the optical component, the probe light forms emergent light after irradiating the sample. The emergent light can be collected by a photoelectric detection device (such as a photoelectric sensor) and converted into a photoelectric signal, and the photoelectric signal can comprise physical quantities such as intensity, light beam directivity and the like.
Step S30: and acquiring photo-thermal signals of the emergent light according to the photoelectric signals, and acquiring unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signals.
Specifically, data analysis processing is carried out on the obtained photoelectric signals, and the photoelectric signals are converted into photo-thermal signals. The photothermal signal may comprise an infrared band-specific signal of the emitted light. Based on the thermal imaging principle, the unit area imaging data of the detection light irradiating sample is obtained according to the photo-thermal signal. The unit area may refer to an area of the sample irradiated with the probe light without changing the angle and displacement, and the unit area imaging data may refer to imaging data obtained from the outgoing light without changing the angle and displacement of the probe light.
Step S40: and controlling an irradiation light path to scan the sample to obtain a plurality of unit area imaging data until the target area imaging data of the sample is obtained according to the unit area imaging data.
In particular, the terminal may control the position and angle of the illumination path to scan the sample. The illumination optical path may generally include an exit optical path for probe light and an exit optical path for mid-infrared pump light. The control of the irradiation light path for scanning may include changing only the emergent light path of the probe light, changing only the emergent light path of the mid-infrared pump light, and synchronously changing the emergent light paths of the probe light and the mid-infrared pump light. By repeating the steps of steps S10 to S30, a plurality of unit area imaging data can be obtained until the target area imaging data of the sample can be obtained from the plurality of unit area imaging data. Target region imaging data may refer to imaging data of the entire area of the sample or a designated target region. The unit area is usually smaller than or equal to the target area, and the unit area imaging data can be combined to obtain target area imaging data.
In some embodiments, in conjunction with FIG. 3, the circle may represent a unit area. And controlling the irradiation light path of the detection light to be adjusted along two orthogonal directions of an X axis and a Y axis of the sample so as to obtain a plurality of unit area imaging data, and processing the data to obtain target area imaging data so as to complete two-dimensional imaging of the target area of the sample.
In some embodiments, in conjunction with FIG. 4, a row of circles may represent a unit area. And controlling the irradiation light path of the detection light to be adjusted along the Y axis of the sample so as to obtain a plurality of unit area imaging data, and obtaining target area imaging data through data processing so as to finish two-dimensional imaging of the target area of the sample.
In some embodiments, in conjunction with fig. 5, the circles in rows and columns 5 collectively may represent a unit area. And controlling the probe light to irradiate the sample, and directly obtaining the imaging data of the target area, thereby completing the two-dimensional imaging of the target area of the sample. When the unit area is smaller than the target area, the irradiation light path of the detection light can be controlled to be adjusted along two orthogonal directions of an X axis and a Y axis of the sample to obtain a plurality of unit area imaging data, and the target area imaging data is obtained through data processing, so that the two-dimensional imaging of the target area of the sample is completed.
In the optical imaging method, the sample is irradiated by the intermediate infrared pulse light to generate a thermal effect, emergent light of the sample is irradiated by the acquired detection light, and the sample is subjected to two-dimensional imaging according to a photo-thermal signal of the emergent light, so that the sample is imaged with high precision; the detection light supports continuous light and pulse light, meets the imaging requirements of different occasions and meets the requirements of different imaging accuracies; meanwhile, the irradiation light path of the detection light is controlled, and the sample is scanned, so that two-dimensional imaging of a target area of the sample is realized.
In some embodiments of the present disclosure, the emerging light is reflected light or transmitted light of the probe light illuminating the sample. Specifically, the emergent light of the sample irradiated by the detection light may be reflected light after the sample is irradiated by the detection light, or may be transmitted light which transmits the sample without changing the direction of the light path after the sample is irradiated by the detection light.
In some embodiments of the present disclosure, in the case that the probe light is continuous light, as shown in fig. 6, the step S30 includes:
step S32: and carrying out first filtering processing on the photoelectric signal to obtain a first filtering signal, and extracting a harmonic component of the first filtering signal.
Specifically, the photoelectric signal may be subjected to fourier transform, the transformed signal may be subjected to a first filtering process to obtain a first filtered signal, and then a harmonic component of the first filtered signal may be extracted. The first filtering process may include, but is not limited to, low-pass filtering, band-pass filtering, amplification filtering, matched filtering, and other digital signal processing. FIG. 7 is a schematic diagram showing (a) a pulse waveform of the mid-IR pump light; (b) is a waveform schematic diagram of the photoelectric signal; (c) The signal diagram after the Fourier transform is carried out on the photoelectric signal.
Step S34: and carrying out second filtering processing on the harmonic component to obtain a second filtering signal.
Step S36: obtaining the photothermal signal according to the second filtered signal;
specifically, each harmonic component is subjected to second filtering processing to obtain a second filtered signal, and the second filtering processing may refer to the first filtering processing or other filtering processing manners. The photothermal signal is then extracted from the second filtered signal.
Step S38: and acquiring unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal.
Specifically, imaging data per unit area of the sample irradiated with the probe light is obtained from the photothermal signal based on the principle of thermal imaging.
The embodiment processes the acquired photoelectric signals by digital signals and improves prime ministry accuracy and sensitivity by filtering twice; and each harmonic component is captured for imaging, so that the imaging resolution is greatly improved, and the imaging speed is improved.
In some embodiments of the present disclosure, the second filtering process includes narrowband filtering the harmonic component. Specifically, the second filtering process may be narrow-band filtering, that is, applying narrow-band filtering to each harmonic component so that each harmonic component participates in the imaging process.
In the embodiment, the nanosecond resolution of the dynamic process of the single-pulse photothermal signal is recorded by carrying out narrow-band filtering on harmonic components; and meanwhile, the imaging sensitivity and the imaging speed are improved.
In some embodiments of the present disclosure, in the case that the detection light is pulsed light, as shown in fig. 8, the detection light includes a first pulse I within a single pulse period0And a second pulse I1The first pulse is set in the time period before the rising edge of the middle infrared pumping light, and the second pulse is set in the time period after the falling edge of the middle infrared pumping light. The terminal may control the first pulse and the second pulse within a single pulse period of the probe light by the control pulse signal.
Step S20 includes: and acquiring a first photoelectric signal of the first pulse, and acquiring a second photoelectric signal of the second pulse. Specifically, a first photoelectric signal of a first pulse is acquired, and a second photoelectric signal of a second pulse is acquired. The variation of the photoelectric signal before and after the irradiation of the mid-infrared pump light can be calculated according to the difference value of the first photoelectric signal and the second photoelectric signal.
In this embodiment, the detection light triggered by the pulse collects the first photoelectric signal of the first pulse and collects the second photoelectric signal of the second pulse, so as to calculate the variation of the photoelectric signal before and after irradiation of the mid-infrared pump light, thereby improving the imaging accuracy and reducing the energy consumption of the detection light.
It should be understood that, although the steps in the flowcharts related to the embodiments as described above are sequentially displayed as indicated by arrows, the steps are not necessarily performed sequentially as indicated by the arrows. The steps are not performed in the exact order shown and described, and may be performed in other orders, unless explicitly stated otherwise. Moreover, at least a part of the steps in the flowcharts related to the embodiments described above may include multiple steps or multiple stages, which are not necessarily performed at the same time, but may be performed at different times, and the execution order of the steps or stages is not necessarily sequential, but may be performed alternately or alternately with other steps or at least a part of the steps or stages in other steps.
Based on the same inventive concept, the disclosed embodiment also provides an optical imaging device for realizing the optical imaging method. The solution of the problem provided by the apparatus is similar to the solution described in the above method, so the specific limitations in one or more embodiments of the optical imaging apparatus provided below can be referred to the limitations of the optical imaging method in the above, and are not described herein again.
The apparatus may include systems (including distributed systems), software (applications), modules, components, servers, clients, etc. that use the methods described in embodiments of the present specification in conjunction with any necessary apparatus to implement the hardware. Based on the same innovative concept, embodiments of the present disclosure provide an apparatus in one or more embodiments as described in the following embodiments. Since the implementation scheme of the apparatus for solving the problem is similar to that of the method, the specific implementation of the apparatus in the embodiment of the present specification may refer to the implementation of the foregoing method, and repeated details are not repeated. As used hereinafter, the term "unit" or "module" may be a combination of software and/or hardware that implements a predetermined function. Although the means described in the embodiments below are preferably implemented in software, an implementation in hardware, or a combination of software and hardware is also possible and contemplated.
In some embodiments of the present disclosure, as shown in fig. 9, there is provided an optical imaging apparatus, which may be the aforementioned terminal, or a module, component, device, unit, etc. integrated with the terminal. The apparatus Z00 may comprise:
the light source module Z10 is used for controlling the irradiation of detection light and mid-infrared pump light on the sample, wherein the detection light is continuous light or pulse light, and the mid-infrared pump light is mid-infrared pulse light with a set wavelength;
the optical detection module Z20 is used for collecting photoelectric signals of emergent light after the sample is irradiated by the probe light;
the data processing module Z30 is used for obtaining a photo-thermal signal of the emergent light according to the photoelectric signal and obtaining unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal;
and the scanning module Z40 is used for controlling the irradiation light path of the detection light to scan the sample to obtain a plurality of unit area imaging data until the target area imaging data of the sample is obtained according to the unit area imaging data.
In some embodiments of the present disclosure, the exit light is reflected light or transmitted light of the probe light irradiating the sample.
In some embodiments of the present disclosure, as shown in fig. 10, in the case that the probe light is a continuous light, the data processing module Z30 includes:
the first filtering unit Z32 is configured to perform first filtering processing on the photoelectric signal to obtain a first filtered signal, and extract a harmonic component of the first filtered signal;
the second filtering unit Z34 is configured to perform second filtering processing on the harmonic component to obtain a second filtered signal;
a photothermal signal unit Z36 for obtaining the photothermal signal from the second filtered signal;
and the imaging unit Z38 is used for obtaining imaging data of a unit area of the sample irradiated by the detection light according to the photothermal signal.
In some embodiments of the present disclosure, the second filtering process includes narrowband filtering the harmonic component.
In some embodiments of the present disclosure, in a case where the detection light is pulsed light, the detection light includes a first pulse and a second pulse within a single pulse period, the first pulse is set to a time period before a rising edge of the mid-infrared pump light, and the second pulse is set to a time period after a falling edge of the mid-infrared pump light;
the optical detection module comprises a pulse unit for acquiring a first photoelectric signal of the first pulse and acquiring a second photoelectric signal of the second pulse.
The respective modules in the optical imaging apparatus described above may be implemented in whole or in part by software, hardware, and a combination thereof. The modules can be embedded in a hardware form or independent from a processor in the computer device, and can also be stored in a memory in the computer device in a software form, so that the processor can call and execute operations corresponding to the modules. It should be noted that, the division of the modules in the embodiment of the present disclosure is illustrative, and is only one logical function division, and there may be another division manner in actual implementation.
In some embodiments of the present disclosure, as shown in fig. 11, an optical imaging system is provided. The system X00 may include:
the intermediate infrared pump light source X10 is used for emitting intermediate infrared pulse light with set wavelength to irradiate a sample;
the detection light source X20 is used for emitting detection light to irradiate the sample, and the detection light is continuous light or pulse light;
a photoelectric detection device X30 for collecting photoelectric signals of the detection light;
the data acquisition device X40 is used for acquiring a photoelectric signal output by the photoelectric detection device and acquiring a photo-thermal signal of the emergent light according to the photoelectric signal;
the upper computer X50 is electrically connected with the intermediate infrared pump light source X10, the detection light source X20, the photoelectric detection device X30 and the data acquisition device X40, and the upper computer X50 is used for imaging according to the photo-thermal signals output by the data acquisition device X40. Specifically, the upper computer X50 may be the aforementioned terminal. The upper computer can realize the optical imaging method.
In some embodiments of the present disclosure, with reference to fig. 12, the system further includes a light path module, where in the case that the emergent light is reflected light of the detection light irradiating the sample, the light path module includes the objective lens 300, the first light path component 410, the beam splitter 500, and the first galvanometer scanner 610, and the detection light reaches the objective lens 300 through the beam splitter 500, the first galvanometer scanner 610, and the first light path component 410, and irradiates the sample through the objective lens 300. The sample may be disposed on a transparent stage 200. The reflected light of the probe light on the sample reaches the photodetection device X30 through the objective lens 300, the first optical path component 410, the first galvanometer mirror 610, and the beam splitter 500. The first optical path component 410 may include an optical element or combination of optical elements such as a lens, polarizer, etc. In this embodiment, the first optical path component includes a first galvanometer mirror and two optical path lenses. It should be noted that other optical elements or combinations of optical elements may also be included in the optical path module.
In some embodiments of the present disclosure, with reference to fig. 13, the system further includes a light path module, where in the case that the emergent light is transmitted light of the detection light irradiating the sample, the light path module includes a first scanning galvanometer 610, a second light path component 420, the objective lens 300, and a beam splitter 500, the detection light reaches the objective lens 300 through the first scanning galvanometer 610 and the second light path component 420, and irradiates the sample through the objective lens 300; the transmitted light of the probe light irradiating the sample reaches the photodetection device X30 through the spectroscope 500. The second optical path component 420 may be the same as or different from the aforementioned first optical path component 410, and the constituent elements of the second optical path component in this embodiment are the same as those of the first optical path component.
In some embodiments of the present disclosure, with reference to fig. 14, the system further includes a light path module, where in the case that the emergent light is transmitted light of the detection light irradiating the sample, the light path module includes a first scanning galvanometer 610, a third light path component 430, an objective lens 300, and a beam splitter 500, and the detection light irradiates the sample through the first scanning galvanometer 610 and the beam splitter 500; the transmitted light of the probe light irradiating the sample reaches the photo detection device X30 through the objective lens 300 and the third optical path assembly 430. The third optical path assembly 430 may be the same as or different from the first optical path assembly 410 and the second optical path assembly 420, and the components of the second optical path assembly in this embodiment are the same as those of the first optical path assembly and the second optical path assembly.
In some embodiments of the present disclosure, as shown in fig. 15 to 17, the optical path module further includes a second scanning galvanometer 620, and the second scanning galvanometer 620 is disposed between the mid-infrared pump light source X10 and the sample. By moving the first oscillating scanning mirror 610 and/or the second oscillating scanning mirror 620, three modes of changing only the emergent light path of the detection light, changing only the emergent light path of the mid-infrared pump light, and synchronously changing the emergent light paths of the detection light and the mid-infrared pump light can be realized.
In some embodiments of the present disclosure, in combination with fig. 18, in the case that the detection light is pulsed light, the detection light includes pulsed light with two polarization components. The emergent light reaches the photoelectric detection device through the polarization light splitting component.
Specifically, when the pulse duration of the mid-infrared pump light is in the order of ns or more, the terminal may control the first pulse and the second pulse within a single pulse period of the probe light by the control pulse signal. When the mid-infrared pump light pulse duration is shorter than 1ns, the probe light includes pulsed light having two polarization components. With the polarization splitting assembly 700 shown in fig. 18, the polarization splitting assembly 700 may be disposed in front of the photodetection device X30 so that the outgoing light enters the photodetection device X30 through the polarization splitting assembly 700. The optical path formed by the polarization beam splitter 700 causes the optical path difference between the two polarization components, so that the pulsed probe light reaches the sample surface in the form of two pulses.
Based on the foregoing description of the embodiments of the optical imaging method, in another embodiment provided by the present disclosure, a computer device is provided, which may be a terminal, and the internal structure thereof may be as shown in fig. 19. The computer device comprises a processor, a memory, a communication interface, a display screen and an input device which are connected through a system bus. Wherein the processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device comprises a nonvolatile storage medium and an internal memory. The non-volatile storage medium stores an operating system and a computer program. The internal memory provides an environment for the operation of an operating system and computer programs in the non-volatile storage medium. The communication interface of the computer device is used for carrying out wired or wireless communication with an external terminal, and the wireless communication can be realized through WIFI, a mobile cellular network, NFC (near field communication) or other technologies. The computer program is executed by a processor to implement an optical imaging method. The display screen of the computer equipment can be a liquid crystal display screen or an electronic ink display screen, and the input device of the computer equipment can be a touch layer covered on the display screen, a key, a track ball or a touch pad arranged on a shell of the computer equipment, an external keyboard, a touch pad or a mouse and the like.
It will be appreciated by those skilled in the art that the configurations shown in the figures are block diagrams of only some of the configurations relevant to the present application, and do not constitute a limitation on the computing devices to which the present application may be applied, and that a particular computing device may include more or less components than those shown in the figures, or may combine certain components, or have a different arrangement of components.
Based on the foregoing description of the embodiments of the optical imaging method, in another embodiment provided by the present disclosure, a computer-readable storage medium is provided, on which a computer program is stored, which, when being executed by a processor, performs the steps in the above-mentioned embodiments of the method.
Based on the foregoing description of embodiments of the optical imaging method, in another embodiment provided by the present disclosure, a computer program product is provided, which comprises a computer program, which when executed by a processor, performs the steps in the above-described method embodiments.
It should be noted that the user information (including but not limited to user device information, user personal information, etc.) and data (including but not limited to data for analysis, stored data, presented data, etc.) referred to in the present disclosure are information and data that are authorized by the user or sufficiently authorized by each party.
It will be understood by those skilled in the art that all or part of the processes of the methods of the embodiments described above may be implemented by hardware instructions of a computer program, which may be stored in a non-volatile computer-readable storage medium, and when executed, may include the processes of the embodiments of the methods described above. Any reference to memory, database, or other medium used in the embodiments provided herein may include at least one of non-volatile and volatile memory. The nonvolatile Memory may include Read-Only Memory (ROM), magnetic tape, floppy disk, flash Memory, optical Memory, high-density embedded nonvolatile Memory, resistive Random Access Memory (ReRAM), magnetic Random Access Memory (MRAM), ferroelectric Random Access Memory (FRAM), phase Change Memory (PCM), graphene Memory, and the like. Volatile Memory can include Random Access Memory (RAM), external cache Memory, and the like. By way of illustration and not limitation, RAM can take many forms, such as Static Random Access Memory (SRAM) or Dynamic Random Access Memory (DRAM), among others. The databases involved in the embodiments provided herein may include at least one of relational and non-relational databases. The non-relational database may include, but is not limited to, a block chain based distributed database, and the like. The processors referred to in the embodiments provided herein may be general purpose processors, central processing units, graphics processors, digital signal processors, programmable logic devices, quantum computing based data processing logic devices, etc., without limitation.
In the description herein, references to the description of "some embodiments," "other embodiments," or the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, a schematic description of the above terminology may not necessarily refer to the same embodiment or example.
It is understood that the embodiments of the method described above are described in a progressive manner, and the same/similar parts of the embodiments are referred to each other, and each embodiment focuses on differences from the other embodiments. Reference may be made to the description of other method embodiments for relevant points.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features of the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present disclosure, and the description thereof is specific and detailed, but not construed as limiting the scope of the claims. It should be noted that various changes and modifications can be made by one skilled in the art without departing from the spirit of the disclosure, and these changes and modifications are all within the scope of the disclosure. Therefore, the protection scope of the present disclosure should be subject to the appended claims.
Claims (12)
1. An optical imaging method, characterized in that the method comprises:
controlling detection light and mid-infrared pump light to irradiate a sample, wherein the detection light is continuous light or pulse light, and the mid-infrared pump light is mid-infrared pulse light with a set wavelength;
acquiring a photoelectric signal of emergent light of the sample irradiated by the detection light;
acquiring a photo-thermal signal of the emergent light according to the photoelectric signal, and acquiring unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal;
and controlling an irradiation light path to scan the sample to obtain a plurality of unit area imaging data until the target area imaging data of the sample is obtained according to the unit area imaging data.
2. The method of claim 1, wherein the exit light is reflected light or transmitted light of the probe light impinging on the sample.
3. The method of claim 1, wherein in the case where the probe light is continuous light, said obtaining a photothermal signal of the emerging light from the photoelectric signal, and obtaining imaging data per unit area of the sample illuminated by the probe light from the photothermal signal comprises:
carrying out first filtering processing on the photoelectric signal to obtain a first filtering signal, and extracting a harmonic component of the first filtering signal;
carrying out second filtering processing on the harmonic component to obtain a second filtering signal;
obtaining the photothermal signal from the second filtered signal;
and acquiring unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal.
4. The method according to claim 3, wherein the second filtering process includes narrowband filtering the harmonic component.
5. The method according to claim 1, wherein in the case that the probe light is pulsed light, the probe light includes a first pulse and a second pulse within a single pulse period, the first pulse sets a time period before the rising edge of the mid-infrared pump light, and the second pulse sets a time period after the falling edge of the mid-infrared pump light;
the acquiring of the photoelectric signal of the emergent light after the sample is irradiated by the probe light comprises:
and acquiring a first photoelectric signal of the first pulse, and acquiring a second photoelectric signal of the second pulse.
6. An optical imaging apparatus, characterized in that the apparatus comprises:
the light source module is used for controlling the irradiation of detection light and mid-infrared pump light on the sample, wherein the detection light is continuous light or pulse light, and the mid-infrared pump light is mid-infrared pulse light with a set wavelength;
the optical detection module is used for collecting photoelectric signals of emergent light after the detection light irradiates the sample;
the data processing module is used for obtaining a photo-thermal signal of the emergent light according to the photoelectric signal and obtaining unit area imaging data of the sample irradiated by the detection light according to the photo-thermal signal;
and the scanning module is used for controlling the irradiation light path to scan the sample to obtain a plurality of unit area imaging data until the target area imaging data of the sample is obtained according to the unit area imaging data.
7. An optical imaging system, characterized in that the system comprises:
the intermediate infrared pump light source is used for emitting intermediate infrared pulse light with set wavelength to irradiate a sample;
the detection light source is used for emitting detection light to irradiate a sample, and the detection light is continuous light or pulse light;
the photoelectric detection device is used for collecting a photoelectric signal of the detection light;
the data acquisition device is used for acquiring a photoelectric signal output by the photoelectric detection device and acquiring a photo-thermal signal of the emergent light according to the photoelectric signal;
the upper computer, with well infrared pump light source, detection light source, photoelectric detection device, data acquisition device electric connection, the upper computer is used for according to the light and heat signal of data acquisition device output carries out the formation of image.
8. The system according to claim 7, further comprising an optical path module, in the case that the outgoing light is reflected light of the detection light irradiating the sample, the optical path module comprising an objective lens, a first optical path component, a beam splitter, a first scanning galvanometer, the detection light reaching the objective lens through the beam splitter, the first scanning galvanometer, the first optical path component, irradiating the sample through the objective lens; the reflected light of the sample irradiated by the detection light reaches the photoelectric detection device through the objective lens, the first light path component, the first scanning galvanometer and the spectroscope.
9. The system of claim 7, further comprising an optical path module, wherein in the case that the emergent light is transmitted light of the sample irradiated by the probe light, the optical path module comprises a first scanning galvanometer, a second optical path component, an objective lens and a spectroscope, the probe light reaches the objective lens through the first scanning galvanometer and the second optical path component, and the sample is irradiated by the objective lens; the transmitted light of the sample irradiated by the probe light reaches the photoelectric detection device through the spectroscope.
10. The system of claim 7, further comprising a light path module, wherein in the case that the emergent light is transmitted light of the sample irradiated by the probe light, the light path module comprises a first scanning galvanometer, a third light path component, an objective lens and a spectroscope, and the probe light irradiates the sample through the first scanning galvanometer and the spectroscope; the transmitted light of the sample irradiated by the probe light reaches the photoelectric detection device through the objective lens and the third light path component.
11. The system according to any one of claims 8-10, wherein the optical path module further comprises a second scanning galvanometer disposed between the mid-infrared pump light source and the sample.
12. The system according to claim 7, wherein in case the probe light is pulsed light, and the probe light comprises pulsed light of two polarization components; the emergent light reaches the photoelectric detection device through the polarization light splitting component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210721653.3A CN115266597A (en) | 2022-06-24 | 2022-06-24 | Optical imaging method, device and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210721653.3A CN115266597A (en) | 2022-06-24 | 2022-06-24 | Optical imaging method, device and system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115266597A true CN115266597A (en) | 2022-11-01 |
Family
ID=83761956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210721653.3A Pending CN115266597A (en) | 2022-06-24 | 2022-06-24 | Optical imaging method, device and system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115266597A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115855848A (en) * | 2022-11-11 | 2023-03-28 | 浙江大学 | Non-fluorescent molecule super-resolution imaging system based on photothermal relaxation positioning microscope |
-
2022
- 2022-06-24 CN CN202210721653.3A patent/CN115266597A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115855848A (en) * | 2022-11-11 | 2023-03-28 | 浙江大学 | Non-fluorescent molecule super-resolution imaging system based on photothermal relaxation positioning microscope |
| CN115855848B (en) * | 2022-11-11 | 2023-08-18 | 浙江大学 | Non-fluorescent molecule super-resolution imaging system based on photo-thermal relaxation positioning microscope |
| WO2024098935A1 (en) * | 2022-11-11 | 2024-05-16 | 浙江大学 | Non-fluorescent molecular super-resolution imaging system based on photothermal relaxation localization microscope |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Satat et al. | Object classification through scattering media with deep learning on time resolved measurement | |
| Li et al. | Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm | |
| Seo et al. | High-throughput lens-free blood analysis on a chip | |
| Backer et al. | Extending single-molecule microscopy using optical Fourier processing | |
| Schwartz et al. | Superresolution microscopy with quantum emitters | |
| Marx | Brain mapping in high resolution | |
| Zavala et al. | The SCUBA-2 Cosmology Legacy Survey: The EGS deep field I–Deep number counts and the redshift distribution of the recovered Cosmic Infrared Background at 450 and 850 μm | |
| Small et al. | Superresolution localization methods | |
| Totero Gongora et al. | Route to intelligent imaging reconstruction via terahertz nonlinear ghost imaging | |
| Harel et al. | Real-time mapping of electronic structure with single-shot two-dimensional electronic spectroscopy | |
| EP2864468A2 (en) | Imaging or measurement methods and systems | |
| Smith et al. | Submillisecond second harmonic holographic imaging of biological specimens in three dimensions | |
| CN115266597A (en) | Optical imaging method, device and system | |
| Koerner | Models of direct time-of-flight sensor precision that enable optimal design and dynamic configuration | |
| Olivieri et al. | Terahertz nonlinear ghost imaging via plane decomposition: toward near-field micro-volumetry | |
| Lee et al. | A 72× 60 angle-sensitive SPAD imaging array for lens-less FLIM | |
| Guerboukha et al. | Super-resolution orthogonal deterministic imaging technique for terahertz subwavelength microscopy | |
| Musarra et al. | Detection, identification, and tracking of objects hidden from view with neural networks | |
| Lee et al. | CASPI: collaborative photon processing for active single-photon imaging | |
| Bowman et al. | Resonant electro-optic imaging for microscopy at nanosecond resolution | |
| Hutson et al. | Ultrafast four-dimensional coherent spectroscopy by projection reconstruction | |
| Tsikouras et al. | Characterization of SPAD array for multifocal high-content screening applications | |
| Jazani et al. | Computational proposal for tracking multiple molecules in a multifocus confocal setup | |
| Coic et al. | Assessment of essential information in the fourier domain to accelerate raman hyperspectral microimaging | |
| Mazumder et al. | Three-dimensional imaging of biological cells using surface plasmon coupled emission |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20230321 Address after: Unit 209, Building B2, Suzhou Biomedical Industrial Park Phase I, No. 218, Xinghu Street, Suzhou Industrial Park, Suzhou Area, China (Jiangsu) Pilot Free Trade Zone, 215125 Jiangsu Province (the address shall not be used for retail) Applicant after: Zhendian (Suzhou) Medical Technology Co.,Ltd. Applicant after: WEIPENG (SUZHOU) MEDICAL DEVICES Co.,Ltd. Address before: Unit 209, Building B2, Suzhou Biomedical Industrial Park Phase I, No. 218, Xinghu Street, Suzhou Industrial Park, Suzhou Area, China (Jiangsu) Pilot Free Trade Zone, 215125 Jiangsu Province (the address shall not be used for retail) Applicant before: Zhendian (Suzhou) Medical Technology Co.,Ltd. |
|
| TA01 | Transfer of patent application right |