CN115247207A - 一种用于2型糖尿病鉴别的肠道微生物基因标记物组合及其应用 - Google Patents

一种用于2型糖尿病鉴别的肠道微生物基因标记物组合及其应用 Download PDF

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CN115247207A
CN115247207A CN202011185119.2A CN202011185119A CN115247207A CN 115247207 A CN115247207 A CN 115247207A CN 202011185119 A CN202011185119 A CN 202011185119A CN 115247207 A CN115247207 A CN 115247207A
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尚进
张一丁
郭瑞雪
崔文
郑雪君
赵占正
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Abstract

本发明属于微生物医药技术领域，具体涉及一种用于2型糖尿病鉴别的肠道微生物基因标记物组合及其应用，尤其是一种将糖尿病患者或高风险糖尿病患者从正常人群中鉴别出来的肠道微生物基因标记物组合。本发明所述肠道微生物基因标记物组合共由246个不同的OTU组成，是一种可以区别糖尿病人或者糖尿病高风险病人与正常人群的肠道微生物标记物模型。利用这246种差异OTU，并通过构建ROC曲线，成功的获得了一种灵敏度和特异度均较高的糖尿病人群的无创筛选的标记物组合，并进一步建立了一种灵敏度和特异度均较高的鉴别模型，可以有效的将糖尿病人与健康人群进行区分，显示出了良好的鉴别能力。

Description

一种用于2型糖尿病鉴别的肠道微生物基因标记物组合及其 应用
技术领域
本发明属于微生物医药技术领域，具体涉及一种用于2型糖尿病鉴别 的肠道微生物基因标记物组合，尤其是一种将糖尿病患者或高风险糖尿病 患者从正常人群中鉴别出来的肠道微生物基因标记物组合。
背景技术
糖尿病是目前世界上最常见的代谢性疾病之一，影响着全世界约4亿 多人的健康。据统计，截至2017年，我国约有1.144亿糖尿病患者(20-79 岁)，而随着我国经济发展，生活水平提高及人口老龄化，糖尿病患病率也 明显上升，预计至2045年，这一数字将会达到1.343亿。目前，我国诊断 糖尿病人的方法主要以测量血糖为主，然而，这种方法可能存在一定的偶 然性，或因测量仪器的偏差，或测量人员的操作不当，而存在未能将初期 或潜在高危糖尿病人诊断出来；而且，部分具有凝血功能障碍或者合并有 其他禁忌证的患者也无法进行采血操作。因此，迫切需要开发更为安全的 并具有高灵敏度和特异度的糖尿病预测或诊断手段。
人类的肠道内定殖着数量庞大的肠道细菌(约10万亿个)，且种类丰 富、结构复杂。据研究，肠道菌群的组成和稳态与人体健康息息相关，对 宿主的消化吸收，代谢功能，免疫反应等方面产生着重要的影响。据已有 研究报道，肠道菌群更与许多疾病有着密切的联系，如直肠癌，肝硬化， 干细胞肝癌，糖尿病等。基于肠道菌群的强大潜力，许多研究已经表明肠 道菌群可能有成为新的诊断疾病方式的能力。如中国专利CN105378739A 公开了一种利用宏基因组簇(MGCs)来鉴定患有或有风险发生II型糖尿病 的个体的模型。但是，由于宏基因组测序过程繁杂且时间成本高，且测序 针对的菌属众多，标记物的特异性不高。因此，急需开发一种灵敏度、特 异度均较高的肠道微生物基因标记物，用于临床快速准确的鉴别及筛选糖 尿病患者。
发明内容
为此，本发明所要解决的技术问题在于提供一种用于2型糖尿病鉴别 的肠道微生物基因标记物组合，其包括246种OTU在正常人组和糖尿病 人组中间存在统计学差异，可用于临床糖尿病患者的筛选及诊断；
本发明所要解决的第二个技术问题在于提供所述肠道微生物基因标记 物在2型糖尿病患者的临床筛选及诊断中的应用。
为解决上述技术问题，本发明所述的一种用于2型糖尿病鉴别的肠道 微生物基因标记物组合，包括如下表中所示的246种OTU的组合：
Figure BDA0002748732980000021
Figure BDA0002748732980000031
Figure BDA0002748732980000041
Figure BDA0002748732980000051
优选的，各所述OTU标记物包括如下表中所示序号的核苷酸序列结构：
Figure BDA0002748732980000052
Figure BDA0002748732980000061
Figure BDA0002748732980000071
本发明还公开了所述微生物基因标记物组合用于制备糖尿病患者与健 康人鉴别诊断试剂，或者，糖尿病患者的筛查或是辅助诊断，或者，糖尿 病微生物靶向治疗药物的用途。
具体的，所述糖尿病包括2型糖尿病。
本发明还公开了一种用于糖尿病鉴别及诊断的试剂盒，包括所述的肠 道微生物基因标记物组合的引物。
具体的所述的试剂盒，所述引物包括341F_805R，其插入长度为465bq， 测序长度为2*300bp，具体结构为：
上游引物：341F 5’-CCTACGGGNGGCWGCAG-3’；
下游引物：805R5’-GACTACHVGGGTATCTAATCC-3’。
本发明还公开了一种构建预测及辅助诊断糖尿病的模型的方法，包括 如下步骤：
(1)收集符合要求的糖尿病人和健康人的大便完成16S rRNA基因测 序；
(2)将获得的基因序列，依据97％的相似性完成OTU聚类以及丰度 测定；进一步采用RDP classifier贝叶斯算法对97％相似水平的OTU代表 序列进行分类学分析，并分别在各个分类水平统计各样品的群落组成；
(3)基于步骤(2)的结果，通过统计学分析，将有统计学意义的且 能够准确区分糖尿病肾病和正常人的OTU筛选出来；
(4)利用步骤(3)筛选出的OTU结果，分别计算在糖尿病患者和正 常人组富集的，且有统计学意义的OTU，并将两类OTU分别相加求和；利 用两组OTU总差值和，构建ROC曲线，从而确定此OTU组合标记物对于 糖尿病和正常人的区别能力。
具体的，所述的预测及辅助诊断糖尿病的模型的构建方法，还包括对 待诊断患者的粪便按照权利要求1或2所述微生物基因标记物进行基因检 测的步骤，以及，对诊断结果进行验证的步骤。
具体的，所述246种OTU组成中，共有56种在糖尿病患者中增多， 190种在糖尿病患者中减少。
本发明还公开了一种用于微生物靶向治疗糖尿病的组合物，包括如权 利要求1或2所述的微生物基因标记物组合，即通过增加或减少相应的所 述标记物的相对含量而治疗或者控制糖尿病，对糖尿病进行早期干预，从 而控制糖尿病的病情，预防并发症的出现。
本发明进一步为治疗糖尿病提供了一种新思路，即通过菌群移植，或 使用相关药物，改变或者控制肠道菌群，从而达到治疗糖尿病，控制糖尿 病发展的目的。
本发明进一步可以用于对患有糖尿病的病人的一种验证方式，部分正 常人可能存在饮食不当，测量方式不当等原因，导致误诊为糖尿病，此时 运用本发明可以对原诊断的正确性进行验证。
本发明所述肠道微生物基因标记物组合共由246个不同的OTU组成， 是一种可以区别糖尿病人或者糖尿病高风险病人与正常人群的肠道微生物 标记物模型。待测患者的样本经过16S rRNA测序后,在所筛选的3115种 OTU中，共有246种OTU在正常人组和糖尿病人组中间存在统计学差异。 利用这246种差异OTU，并通过构建ROC曲线，成功的获得了一种灵敏 度和特异度均较高的糖尿病人群的无创筛选的标记物组合，并进一步建立 了一种灵敏度和特异度均较高的鉴别模型，可以有效的将糖尿病人与健康 人群进行区分，显示出了良好的鉴别能力。根据这些有差异性的菌群，可 以将一些检查时可能存在血糖正常的糖尿病患者或者有糖尿病高发倾向的 人从正常人群众筛查并鉴别出来。
本发明所述方案，可主要用于2型糖尿病(DM)患者与正常人的鉴别， 利用16SrRNA基因测序，将所筛查人群的肠道菌群的微生物谱分析并鉴定 出来，进一步进行统计学分析，将一些有差异性的菌群在OTU的分类水平 上鉴别出来。本发明方案利用肠道菌群标记物预测个体有无糖尿病或高糖 尿病风险的模型，可广泛用于正常人群的糖尿病筛查。尤其对于许多有抽 血禁忌证的患者，如服用大量抗凝药物，或者凝血因子缺乏等凝血功能障碍的患者，利用本发明可以进行无创的糖尿病辅助诊断；可有效避免使用 血糖仪进行检查时，可能会由于设备老化，或是操作者操作不当等问题， 没有将实际患有糖尿病的患者筛查出来，而且与血液中的血糖浓度相比也 更加稳定，适用于所有正常人，也更适用于有出血倾向或有抽血检查禁忌 证的患者；本发明相较常规抽血检查相比，完全无创；同时，由于肠道菌 群是一个相对稳定的组成及相对较为长周期的改变，不会出现极端的结果 偏差，灵敏度和特异度均特异性较强，结果真实可靠。
附图说明
为了使本发明的内容更容易被清楚的理解，下面根据本发明的具体实 施例并结合附图，对本发明作进一步详细的说明，其中，
图1为本发明预测及辅助诊断糖尿病的模型的构建流程图；
图2为获得的ROC曲线结果分析图。
具体实施方式
下列实施例中所用方法如无特别说明，均为常规方法。下列实施例中 所需要的材料或试剂，如无特殊说明均为公开商业途径获得。
实施例1
临床共检测了137名2型糖尿病的患者和179名正常健康人群的大便 相对微生物含量。所有的入选人群随机分为发现队列(糖尿病病人＝102， 正常人＝131)和验证队列(糖尿病病人＝35，正常人＝48)。
如图1所示的流程图，基于高通量测序数据，在发现队列中建立糖尿 病患者和健康人群的微生物区别模型，
样本收集：按照前瞻性临床试验的设计原则，本研究方案得到了郑州 大学第一附属医院伦理委员会的批准。在患者签署知情同意书后，在住院 的第二天清晨，收集患者的新鲜大便样本，收集后先放入装有冰袋的冰盒 中，半小时内送往郑州大学生物样本库，入库后立即存于负80摄氏度冰箱 中，运输过程中避免反复冻存；除此步骤外，其他步骤均在上海illumina 平台完成，共收集到137名2型糖尿病的患者和179名正常健康人群的大 便样本，其中发现队列234人(糖尿病病人＝102，正常人＝131)用于模型 的开发验证，验证队列83人(糖尿病病人＝35，正常人＝48)为模型的实例 验证。
通过对发现队列收集的样本提取微生物总DNA，进行肠道菌群的 16SrDNA Miseq测序，DNA抽提操作具体包括如下步骤：
(1)化学裂解细胞膜及细胞核膜：每个样品加790ul的裂解液(4mmol/L 硫氰酸胍，250ul；10％N-月桂酰肌氨酸,40ul；5％N-月桂酰肌氨酸,0.1mmol/L PBS缓冲液[pH 8.0],500ul)，剧烈涡旋后70℃下孵育1h；
(2)物理击打法(Beads-Beating)充分裂解细胞膜及细胞核膜：孵育 结束后加入玻璃珠(0.1mm，500-750ul)混合，击打10min(25HZ/S)；
(3)后续提取按照抽提试剂盒的说明书进行(E.Z.N.A.
Figure RE-GDA0003066546220000101
DNA Kit)；
(4)第一次离心(14000g，3min)取上清置于2ml离心管中，加入200ul 的SP2buffer，涡旋后冰浴5min；
(5)第二次离心(14000g，3min)取上清置于新的2ml离心管中，加 入200ul的cHTRReagent(用前需摇匀)，混匀后室温放置2min；
(6)第三次离心(14000g，2min)取上清500ul置于新的2ml离心管 中，加入20ul蛋白酶K(20mg/ml)，500ul的BL buffer，涡旋混匀，70℃ 下孵育10min；孵育后加入500ul的无水乙醇，涡旋后进行过柱：将
Figure BDA0002748732980000111
DNA迷你柱插入2mL收集管。
以上述每个样本提取的DNA为模板进行16S rRNA基因的扩增，测序 引物序列包括：
上游引物：341F 5’-CCTACGGGNGGCWGCAG-3’；
下游引物：805R5’-GACTACHVGGGTATCTAATCC-3’。
PCR扩增与测序操作具体包括如下步骤：
以每个样本提取的DNA为模板，扩增16S rRNA基因的V3-V4区域， 反应条件如下：
Figure RE-GDA0003066546220000112
将PCR产物按相同比例进行混合，在Illumina Miseq平台完成测序。
实施例2OTU聚类与注释
在16S rRNA高通量测序后，将所测得序列进行归类操作，将序列按照 相似性分归为许多操作单元，即一个OTU(同种基因序列分类单元， Operational Taxonomy Units)。可根据不同的相似度水平，对所有序列进行OTU划分，通常在97％的相似水平下的OTU进行生物信息统计分析 (Usearch软件)。将OTU作为基本操作的单元，并使用Silva(SSU123) 16SrRNA数据基础的RDP分类器(http://rdp.cme.msu.edu/)来进行分析注 释。通过RDP分类器贝叶斯算法对97％相似水平的OTU代表序列进行分 类学分析，从而得到每个OTU对应的物种分类信息，并分别在各个分类水 平：domain(域)，kingdom(界)，phylum(门)，class(纲)，order(目)， family(科)，genus(属)，species(种)统计各样品的群落组成,
群落组成差异分析：我们使用LEfSe分析(Linear discriminant analysisEffect Siz，线性判别分析)评估所有OTU在两组之间丰度的差异，得出所 有LDA>2.0(Linear discriminant analysis)的OTU，共有246种LDA值大 于2.0，其中有56种在糖尿病患者中富集，190种在正常人群中更为富集。 根据已确定归类的细菌，这些OTU分属于5个不同phylum(门)、10个不 同class(纲)、10个不同order(目)、21个不同科family(科)、41个不同 的genus(属)及3种未归类细菌。
经检测可知，在糖尿病患者中不同OUT的增多或减少情况见下表1所 示。
表1OTU的序列结构及变化情况
Figure BDA0002748732980000121
Figure BDA0002748732980000131
Figure BDA0002748732980000141
Figure BDA0002748732980000151
Figure BDA0002748732980000161
Figure BDA0002748732980000171
如上表格中，标注CON的是在CON中更为富集，即在糖尿病中减少 的OTU；标记DM的即为在糖尿病患者中增多的OTU。
实施例3ROC分析
对糖尿病人群和正常人群的测定结果进行分析，依据统计学知识，根 据LDA值大于2.0的共计246种有差异的OTU分为疾病富集组和正常人群 富集组，将两类人群的两组OTU的数量分别相加后，进行两组间别分相减， 再将两组OTU相减的结果求和，计算方式见下表2。
表2计算方式
Figure BDA0002748732980000172
利用每个样本所得差值的上下限、组距以及截断点(阈值，cut-off point)， 按选择的组距间隔列出累积频数分布表，分别计算出所有截断点的敏感性 (Sensitivity)、特异性和假阳性率(1-特异性：Specificity)。以敏感性为纵 坐标代表真阳性率，(1-特异性)为横坐标代表假阳性率，作图绘成ROC曲 线。ROC曲线下面积值(AUC)在1.0和0.5之间，越靠近左上角，诊断 的准确性越高。在AUC>0.5的情况下，AUC越接近于1，说明诊断效果越 好。AUC在0.5-0.7时有较低准确性，AUC在0.7-0.9时有一定准确性，AUC 在0.9以上时有较高准确性。故ROC曲线越靠近左上角,试验的准确性就越 高。最靠近左上角的ROC曲线的点是错误最少的最好阈值，即为cut off值， 其假阳性和假阴性的总数最少，此时，模型的敏感度和特异度的综合水平 是最高的，即模型的诊断效能是最佳的。
构建出ROC模型，计算出其曲线下的面积AUC(见图2中A)。在发 现队列中AUC达到了92.25％(95％置信区间：88.71％-95.79％)，在最佳截 断处，模型的敏感性和特异性分别达到了75.81％和92.22％。体现出了此鉴 别模型较高的诊断效能。
测得本发明所需纳入的246种OTU相对丰度值，通过本发明所建立的 模型，进行检验，判断入组对象是否患有糖尿病或糖尿病高发风险。
实验例4
以验证队列为实验例，对本发明进行进一步说明。
我们在验证队列中对模型进行验证。验证队列共有83人，其中糖尿病 病人35人，正常健康人48人，纳入本模型并进行模型的验证。本发明通 过收集入组对象的粪便样本，抽提微生物总DNA，进行肠道菌群的 16SrDNA Miseq测序。分别鉴别并计算出模型所需的246种OTU的相对丰 度值，并纳入模型进行鉴别。具体包括：
(1)按照前瞻性临床试验的设计原则，如本发明图1所示流程图，该 研究方案得到了郑州大学第一附属医院伦理委员会的批准，所有入组的患 者签署研究方案知情同意书和临床样本收集知情同意书；
(2)收集入组对象(糖尿病患者和健康人群)的粪便样本，按照DNA 的标准抽提方法完成粪便样本中微生物总DNA的抽提，在Illumina MiSeq 平台完成肠道菌群的16SrDNA高通量测序工作；
(3)基于肠道菌群测序数据结果，将验证队列83名入组对象的肠道 菌群进行16SrDNA基因序列分类单元(Operational Taxonomy Units，OTUs) 水平进行归类，并计算出模型所需求的246种OTU的相对丰度；
(4)得到验证队列所需的246微生物基因标记物的相对丰度，根据模 型，将56种在糖尿病中更为富集和190种在正常人中更为富集的OTU分 别相加，求出两类人群中两组OTU的总差值和，并将其纳入之基于发现队 列所建立的模型中，对模型的鉴别能力进行测试；
(5)绘制验证队列组的ROC曲线(如图2中B)，评价该模型的鉴别 效果，其曲线下面积AUC达到90.48％，即在此模型对验证队列的35名糖 尿病患者及48名正常人的区别能力达到90.48％，且敏感度和特异度分别达 到了90.91％和78.72％，验证了本发明所述鉴别模型出色的鉴别能力。
因此，本发明的微生物基因区别模型在2型糖尿病患者和健康人群中 实现了良好的区别能力。
本发明通过上述实施例来说明本发明的详细方法，但本发明并不局限 于上述详细方法，即不意味着本发明必须依赖上述详细方法才能实施。所 属技术领域的技术人员应该明了，对本发明的任何改进，对本发明产品各 原料的等效替换及辅助成分的添加、具体方式的选择等，均落在本发明的 保护范围和公开范围之内。
序列表
<110> 郑州大学第一附属医院
<120> 一种用于2型糖尿病鉴别的肠道微生物基因标记物组合及其应用
<160> 246
<170> SIPOSequenceListing 1.0
<210> 1
<211> 440
<212> DNA
<213> OTU11
<400> 1
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggagc 180
aagcgttgtc cggatttact gggtgtaaag ggtgcgcagg cggaaggcta agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg gtcatctaga gtgtcggagg 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 2
<211> 440
<212> DNA
<213> OTU36
<400> 2
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgggtagtc 440
<210> 3
<211> 440
<212> DNA
<213> OTU41
<400> 3
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgagta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgaggca agtctgatgt 240
gaaagcctgg ggcttaaccc cggaactgca ttggaaactg ctttgctgga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccttgtagtc 440
<210> 4
<211> 460
<212> DNA
<213> OTU58
<400> 4
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg ttagcctgaa ccagccaagt 60
agcgtgaagg atgaaggctc tatgggtcgt aaacttcttt tatataagaa taaagtgcag 120
tatgtatact gttttgtatg tattatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggactggta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
tcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 5
<211> 440
<212> DNA
<213> OTU66
<400> 5
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt 240
gaaatccccg ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga 300
ggggggtaga attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg 360
cgaaggcggc cccctggacg aagactgacg ctgaggctcg aaagtgtggg tagcaaacag 420
gattagatac ccgagtagtc 440
<210> 6
<211> 440
<212> DNA
<213> OTU72
<400> 6
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attgggaaga acaccagtgg 360
cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 7
<211> 440
<212> DNA
<213> OTU77
<400> 7
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg atgaagtatt tcggtatgta aaactctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggtatggca agtcagaagt 240
gaaaacccag ggcttaactc tgggactgct tttgaaactg tcagactgga gtgcaggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttgctggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgtgtagtc 440
<210> 8
<211> 440
<212> DNA
<213> OTU101
<400> 8
cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgcagca agtctgatgt 240
gaaaggcagg ggcttaaccc ctggactgca ttggaaactg ctgtgcttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccttgtagtc 440
<210> 9
<211> 440
<212> DNA
<213> OTU104
<400> 9
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatc tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggtatggca agtctgatgt 240
gaaaggccgg ggctcaaccc cgggactgca ttggaaactg tcacacttga gtgtcggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg acaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 10
<211> 440
<212> DNA
<213> OTU107
<400> 10
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagccccg gctaaatacg tgccagcagc cgcggtaata cgtatggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggtatggca agtcagaagt 240
gaaagcctgg ggctcaaccc cggaattgct tttgaaactg tcaaactaga gtgtcggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggtgg 360
cgaaggcggc ttactggacg accactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgggtagtc 440
<210> 11
<211> 441
<212> DNA
<213> OTU110
<400> 11
cctacgggtg gctgcagtgg ggaatattgc gcaatggggg aaaccctgac gcagcaacgc 60
cgcgtgattg aagaaggcct tcgggttgta aagatcttta attcgggacg aattttgacg 120
gtaccgaaag aataagctcc ggctaactac gtgccagcag ccgcggtaat acgtagggag 180
caagcgttat ccggatttac tgggtgtaaa gggcgcgcag gcgggccggc aagttggaag 240
tgaaatccgg gggcttaacc cccgaactgc tttcaaaact gctggtcttg agtgatggag 300
aggcaggcgg aattccgtgt gtagcggtga aatgcgtaga tatacggagg aacaccagtg 360
gcgaaggcgg cctgctggac attaactgac gctgaggcgc gaaagcgtgg ggagcaaaca 420
ggattagata ccctagtagt c 441
<210> 12
<211> 440
<212> DNA
<213> OTU111
<400> 12
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctgctggact gttactgaca ctgaggcacg aaagcgtggg gagcaaacag 420
gattagatac ccgtgtagtc 440
<210> 13
<211> 441
<212> DNA
<213> OTU124
<400> 13
cctacgggcg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgatgc 60
cgcgtgaggg aagaaggttt taggattgta aacctctgtc tttggggacg aaaaatgacg 120
gtacccaagg aggaagctcc ggctaactac gtgccagcag ccgcggtaat acgtagggag 180
cgagcgttgt ccggaattac tgggtgtaaa gggagcgtag gcgggatcgc aagtcagatg 240
tgaaaactat gggcttaacc cataaactgc atttgaaact gtggttcttg agtgaagtag 300
aggtaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg aacatcagtg 360
gcgaaggcgg cttactggac tgtaactgac actgaggctc gaaagcgtgg ggagcaaaca 420
ggattagata cccgagtagt c 441
<210> 14
<211> 440
<212> DNA
<213> OTU147
<400> 14
cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgaggg aagaaggttt tcggattgta aacctctgtt cttagtgacg ataatgacgg 120
tagctaagga gaaagctccg gctaactacg tgccagcagc cgcggtaata cgtagggagc 180
gagcgttgtc cggatttact gggtgtaaag ggtgcgtagg cggcgaggca agtcaggcgt 240
gaaatctatg ggcttaaccc ataaactgcg cttgaaactg tcttgcttga gtgaagtaga 300
ggtaggcgga attcccggtg tagcggtgaa atgcgtagag atcgggagga acaccagtgg 360
cgaaggcggc ctactgggct ttaactgacg ctgaagcacg aaagcatggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 15
<211> 440
<212> DNA
<213> OTU149
<400> 15
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcgtactaga gtgtcggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 16
<211> 440
<212> DNA
<213> OTU200
<400> 16
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cggaggatcc 180
gagcgttatc cggatttatt gggtttaaag ggagcgtagg cgggttgtta agtcagttgt 240
gaaagtttgc ggctcaaccg taaaattgca gttgatactg gcgaccttga gtgcaacaga 300
ggtaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360
cgaaggcagc tcactagact gcaactgaca ctgatgctcg aaagtgtggg tatcaaacag 420
gattagatac ccctgtagtc 440
<210> 17
<211> 440
<212> DNA
<213> OTU232
<400> 17
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggttttgca agtctgaagt 240
gaaagcccgg ggcttaaccc cgggactgct ttggaaactg taggactaga gtgcaggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 18
<211> 460
<212> DNA
<213> OTU235
<400> 18
cctacgggtg gctgcagtga ggaatattgg tcaatggccg agaggctgaa ccagccaagt 60
cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgcgg 120
gacgtgtccc gttttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240
cggcctttta agtcagcggt gaaagtctgt ggctcaacca tagaattgcc gttgaaactg 300
gggggcttga gtatgtttga ggcaggcgga atgcgtggtg tagcggtgaa atgcatagat 360
atcacgcaga accccgattg cgaaggcagc ctgccaagcc attactgacg ctgatgcacg 420
aaagcgtggg gatcaaacag gattagatac cctagtagtc 460
<210> 19
<211> 440
<212> DNA
<213> OTU245
<400> 19
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggaattact gggtgtaaag ggtgcgtaga cggcaaggca agtctgatgt 240
gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagag attaggaaga acaccggtgg 360
cgaaggcgac tttctggacg aaaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 20
<211> 440
<212> DNA
<213> OTU266
<400> 20
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggaattact gggtgtaaag ggtgcgtagg tggtatggca agtcagaagt 240
gaaaacccag ggcttaactc tgggactgct tttgaaactg tcagactgga gtgcaggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acatcagtgg 360
cgaaggcggc ttactggact gaaactgaca ctgaggcacg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 21
<211> 440
<212> DNA
<213> OTU276
<400> 21
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cgggagaaca agttggaagt 240
gaaatccatg ggctcaaccc atgaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctgctggact gttactgaca ctgaggcacg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 22
<211> 442
<212> DNA
<213> OTU277
<400> 22
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcaacgc 60
cgcgtgaggg aagacggttt tcggattgta aacctctgtt ttcggtgacg aacaaatgac 120
ggtaaccgag taggaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180
gcaagcgtta tccggaatta ctgggtgtaa agggagcgca ggcgggatag caagtcagct 240
gtgaaaacta tgggctcaac ccataaactg cagttgaaac tgttattctt gagtggagta 300
gaggcaagcg gaattccgag tgtagcggtg aaatgcgtag atattcggag gaacaccagt 360
ggcgaaggcg gcttgctggg ctctaactga cgctgaggct cgaaagtgtg gggagcaaac 420
aggattagat accctagtag tc 442
<210> 23
<211> 465
<212> DNA
<213> OTU286
<400> 23
cctacgggag gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtgtg aagaaggcct tcgggttgta aagcactttc agcggggagg aaggcgttaa 120
ggttaataac cttggcgatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtc tgtcaagtcg gatgtgaaat ccccgggctc aacctgggaa ctgcatctga 300
tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacaaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gatacccctg tagtc 465
<210> 24
<211> 443
<212> DNA
<213> OTU344
<400> 24
cctacgggtg gcagcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60
cgcgtgaagg aagaaggctt tcgggttgta aacttctttt gtcagggacg aagcaagtga 120
cggtacctga cgaataagcc acggctaact acgtgccagc agccgcggta atacgtaggt 180
ggcaagcgtt atccggattt actgggtgta aagggcgtgt aggcgggaaa gcaagtcaga 240
tgtgaaaact gtgggctcaa cccacagcct gcatttgaaa ctgtttttct tgagtactgg 300
agaggcagat ggaattccta gtgtagcggt gaaatgcgta gatattagga ggaacaccag 360
tggcgaaggc gatctgctgg acagcaactg acgctgaggc gcgaaagcgt ggggagcaaa 420
caggattaga taccctagta gtc 443
<210> 25
<211> 460
<212> DNA
<213> OTU366
<400> 25
cctacgggtg gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300
gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420
aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460
<210> 26
<211> 447
<212> DNA
<213> OTU368
<400> 26
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagcgacgc 60
cgcgtgcggg atgacggcct tcgggttgta aaccgctttt gatcgggagc aagccttcgg 120
gtgagtgtac ctttcgaata agcaccggct aactacgtgc cagcagccgc ggtaatacgt 180
agggtgcaag cgttatccgg aattattggg cgtaaagggc tcgtaggcgg ttcgtcgcgt 240
ccggtgtgaa agtccatcgc ttaacggtgg atctgcgccg ggtacgggcg ggctggagtg 300
cggtagggga gactggaatt cccggtgtaa cggtggaatg tgtagatatc gggaagaaca 360
ccaatggcga aggcaggtct ctgggccgtt actgacgctg aggagcgaaa gcgtggggag 420
cgaacaggat tagataccct agtagtc 447
<210> 27
<211> 440
<212> DNA
<213> OTU386
<400> 27
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggaatggca agtctgatgt 240
gaaaggccgg ggctcaaccc cgggactgca ttggaaactg ccaatctaga gtaccggagg 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagctaggg tagcgaacgg 420
gattagatac cccagtagtc 440
<210> 28
<211> 460
<212> DNA
<213> OTU405
<400> 28
cctacggggg gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420
aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460
<210> 29
<211> 439
<212> DNA
<213> OTU418
<400> 29
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttatc cggatttatt gggtgtaaag ggcgcgcagg cgggccggca agttggaagt 240
gaaatccggg ggcttaaccc ccgaactgct ttcaaaactg ctggtcttga gtgatggaga 300
ggcaggcgga attccgtgtg tagcggtgaa atgcgtagat atacggagga acaccagtgg 360
cgaaggcggc ctgctggaca ttaactgacg ctgatgctcg aaagtgtggg tatcaaacag 420
gattagatcc cttgtagtc 439
<210> 30
<211> 442
<212> DNA
<213> OTU427
<400> 30
cctacgggag gcagcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60
cgcgtgaagg aagaaggctt tcgggttgta aacttctttt aagagggaag agcagaagac 120
ggtacctctt gaataagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180
gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccgggtgcg caagtcagat 240
gtgaaatctc agggctcaac cctgaaactg catttgaaac tgtgcatctt gagtgccgga 300
gaggtaatcg gaattccttg tgtagcggtg aaatgcgtag atataaggaa gaacaccagt 360
ggcgaaggcg gattactgga cggtaactga cggtgaggcg cgaaagcgtg gggagcaaac 420
aggattagat acccgggtag tc 442
<210> 31
<211> 440
<212> DNA
<213> OTU428
<400> 31
cctacgggag gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240
gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgggtagtc 440
<210> 32
<211> 440
<212> DNA
<213> OTU433
<400> 32
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aagctctatc agcagggaag acagtgacgg 120
tacctgacta agaagcaccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggaagagca agtctgatgt 240
gaaaggctgg ggcttaaccc caggactgca ttggaaactg tttttcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccggtagtc 440
<210> 33
<211> 460
<212> DNA
<213> OTU440
<400> 33
cctacgggag gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
cggtgtggca agtctgatgt gaaaggcatg ggctcaacct gtggactgca ttggaaactg 300
tcatacttga gtgccggagg ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360
attaggagga acaccagtgg cgaaggcggc ttactggaca gtaactgacg ttgaggctcg 420
aaagcgtggg gagcaaacag gattagatac ccttgtagtc 460
<210> 34
<211> 442
<212> DNA
<213> OTU458
<400> 34
cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaaatgac 120
ggtacctgac taagaagcat cggctaaata cgtgccagca gccgcggtaa tacgtatgga 180
gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggcaagg caagtctgat 240
gtgaaaaccc agggcttaac cctgggactg cattggaaac tgtctggctc gagtgccgga 300
gaggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa gaacaccagt 360
ggcgaaggcg gcttactgga cagtaactga cgttgaggct cgaaagcgtg gggagcaaac 420
aggattagat acccgagtag tc 442
<210> 35
<211> 460
<212> DNA
<213> OTU465
<400> 35
cctacggggg gcagcagtga ggaatattgg tcaatggccg agaggctgaa ccagccaagt 60
cgcgtgaagg aagaaggatc tatggtttgt aaacttcttt tataggggaa taaagtggag 120
gacgtgtcct tttttgtatg taccctatga ataagcatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatgc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420
aaagtgtggg tatcaaacag gattagatac cccagtagtc 460
<210> 36
<211> 442
<212> DNA
<213> OTU478
<400> 36
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgaacg aagaagtatt tcggtatgta aagttctatc agcagggaag aagaaatgac 120
ggtacctgac taagaagctc cggctaaata cgtgccagca gccgcggtaa tacgtatgga 180
gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggcggtctta taagtctgat 240
gtgaaagccc ggggctcaac cccgggactg cattggaaac tgtaggacta gagtgtcgga 300
ggggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360
ggcgaaggcg gcttactgga cgattactga cgctgaggct cgaaagcgtg gggagcaaac 420
aggattagat accccagtag tc 442
<210> 37
<211> 440
<212> DNA
<213> OTU479
<400> 37
cctacgggtg gctgcagtgg gggatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240
gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctaga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 38
<211> 460
<212> DNA
<213> OTU482
<400> 38
cctacgggtg gctgcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgaaggtcc tacggattgt aaacttcttt tataagggaa taaaccctcc 120
cacgtgtggg agcttgtatg taccttatga ataagcatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatgc gagcgttatc cggatttatt gggtttaaag ggagcgcaga 240
cgggtcgtta agtcagctgt gaaagtttgg ggctcaacct taaaattgca gttgatactg 300
gcgtccttga gtgcggttga ggtgtgcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc acactaatcc gtaactgacg ttcatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 39
<211> 440
<212> DNA
<213> OTU492
<400> 39
cctacgggtg gctgcagtcg agaatcattc acaatggggg aaaccctgat ggtgcgacgc 60
cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120
tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaaa cgtagggtgc 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggaccggca agttggaagt 240
gaaatccatg ggctcaaccc gtgaattgct ttcaaaactg ctggccttga gtagtgcaga 300
ggtaggtgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcgac ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420
gattagatac ccttgtagtc 440
<210> 40
<211> 440
<212> DNA
<213> OTU493
<400> 40
cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaaaccatg ggctcaaccc ataaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 41
<211> 440
<212> DNA
<213> OTU507
<400> 41
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg atgaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggtgcgtagg cggctttgca agtcagatgt 240
gaaatctatg ggctcaaccc atagcctgca tttgaaactg cagagcttga gtgaagtaga 300
ggcaggcgga attccccgtg tagcggtgaa atgcgtagag atggggagga acaccagtgg 360
cgaaggcggc ctgctgggct ttaactgacg ctgaggcacg aaagcgtggg tagcaaacag 420
gattagatac ccgagtagtc 440
<210> 42
<211> 460
<212> DNA
<213> OTU513
<400> 42
cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagtattc 120
cacgtgtggg attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatgc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gtttccttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactagact gcaactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 43
<211> 460
<212> DNA
<213> OTU517
<400> 43
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg taagcctgaa ccagccaagt 60
cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgcgg 120
gacgtgtccc gttttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300
gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactagact gttactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 44
<211> 460
<212> DNA
<213> OTU530
<400> 44
cctacggggg gcagcagtga ggaatattgg tcaatgggcg cgagcctgaa ccagccaagt 60
cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgcgg 120
gacgtgtccc gttttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac ccgtgtagtc 460
<210> 45
<211> 465
<212> DNA
<213> OTU531
<400> 45
cctacgggag gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa 120
agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag gatccgagcg ttatccggat ttattgggtt taaagggagc 240
gtagatggat gtttaagtca gttgtgaaag tttgcggctc aaccgtaaaa ttgcagttga 300
tactggatat cttgagtgca gttgaggcag gcggaattcg tggtgtagcg gtgaaatgct 360
tagatatcac gaagaactcc gattgcgaag gcagcctgct aagctgcaac tgacattgag 420
gctcgaaagg gtgggtatca aacaggatta gataccccag tagtc 465
<210> 46
<211> 460
<212> DNA
<213> OTU536
<400> 46
cctacgggag gcagcagtga ggaatattgg tcaatggacg caagtctgaa ccagccatgc 60
cgcgtgcagg atgacggctc tatgagttgt aaactgcttt tgtactaggg taaactcacc 120
tacgtgtagg tgactgaaag tatagtacga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggattc aagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cggacgctta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
ggtgtcttga gtacagtaga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctggact gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460
<210> 47
<211> 460
<212> DNA
<213> OTU548
<400> 47
cctacgggtg gctgcagtga ggaatattgg tcaatggacg cgagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagttgtc 120
cacgtgtgga tttttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gcagtcttga gtgcagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggagt gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 48
<211> 440
<212> DNA
<213> OTU569
<400> 48
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240
gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 49
<211> 439
<212> DNA
<213> OTU587
<400> 49
cctacgggtg gcagcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtaggtggc 180
gagcgttgtc cggaattatt gggcgtaaag agcatgtagg cggcttaata agtcgagcgt 240
gaaaatgcgg ggctcaaccc cgtatggcgc tggaaactgt taggcttgag tgcaggagag 300
gaaaggggaa ttcccagtgt agcggtgaaa tgcgtagata ttgggaggaa caccagtggc 360
gaaggcgcct ttctggactg tgtctgacgc tgagatgcga aagccagggt agcgaacggg 420
attagatacc cctgtagtc 439
<210> 50
<211> 440
<212> DNA
<213> OTU597
<400> 50
cctacgggcg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgagaca agtctgaagt 240
gaaagcccgg ggctcaaccc cgggactgct ttggaaactg ccttgctaga gtgctggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccctgtagtc 440
<210> 51
<211> 460
<212> DNA
<213> OTU606
<400> 51
cctacgggcg gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttact gggtgtaaag ggagcgtaga 240
cggcaaggca agtctgatgt gaaaacccag ggcttaaccc tgggactgca ttggaaactg 300
tctggctcga gtgccggaga ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360
attaggaaga acaccagtgg cgaaggcggc ttgctggaca gtaactgacg ttcaggctcg 420
aaagcgtggg gagcaaacag gattagatac cctagtagtc 460
<210> 52
<211> 465
<212> DNA
<213> OTU609
<400> 52
cctacgggtg gcagcagtgg ggaattttgg acaatgggcg caagcctgat ccagctattc 60
cgcgtgtggg atgaaggccc tcgggttgta aaccactttt gtagagaacg aaaagacacc 120
atcgaataaa tggtgttgct gacggtactc taagaataag caccggctaa ctacgtgcca 180
gcagccgcgg taatacgtag ggtgcgagcg ttaatcggaa ttactgggcg taaagggtgc 240
gcaggcggtt gagtaagaca gatgtgaaat ccccgagctt aactcgggaa tggcatatgt 300
gactgctcga ctagagtgtg tcagagggag gtggaattcc acgtgtagca gtgaaatgcg 360
tagatattag gaggaacacc agtggcgaag gcggcttact ggacgataac tgacgctgag 420
gctcgaaagc gtggggagca aacaggatta gataccctag tagtc 465
<210> 53
<211> 465
<212> DNA
<213> OTU625
<400> 53
cctacgggtg gcagcagtag ggaatcttcg gcaatggacg gaagtctgac cgagcaacgc 60
cgcgtgagtg aagaaggttt tcggatcgta aagctctgtt gtaagagaag aacgagtgtg 120
agagtggaaa gttcacactg tgacggtatc ttaccagaaa gggacggcta actacgtgcc 180
agcagccgcg gtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag 240
cgcaggcggt tagataagtc tgaagttaaa ggctgtggct taaccatagt acgctttgga 300
aactgtttaa cttgagtgca agaggggaga gtggaattcc atgtgtagcg gtgaaatgcg 360
tagatatatg gaggaacacc ggtggcgaaa gcggctctct ggcttgtaac tgacgctgag 420
gctcgaaagc gtggggagca aacaggatta gataccctag tagtc 465
<210> 54
<211> 460
<212> DNA
<213> OTU634
<400> 54
cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120
cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttact gggtgtaaag ggagcgtaga 240
cggatgggca agtctgatgt gaaaacccgg ggctcaaccc cgggactgca ttggaaactg 300
tctggctcga gtgccggaga ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360
attaggaaga acaccagtgg cgaaggcggc ttactggact gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cccggtagtc 460
<210> 55
<211> 440
<212> DNA
<213> OTU656
<400> 55
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag agattgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240
gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 56
<211> 440
<212> DNA
<213> OTU671
<400> 56
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgatta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240
gaaagactgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcgga ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccggtagtc 440
<210> 57
<211> 440
<212> DNA
<213> OTU676
<400> 57
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cggaggatcc 180
gagcgttatc cggatttatt gggtttaaag ggagcgtaga tggatgttta agtcagttgt 240
gaaagtttgc ggctcaaccg taaaattgca gttgatactg gatgtcttga gttcagttga 300
ggcaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360
cgaaggcagc ctgctaatct gcgactgaca ttgaggctcg aaagtgtggg tatcaaacag 420
gattagatac ccgggtagtc 440
<210> 58
<211> 459
<212> DNA
<213> OTU678
<400> 58
cctacgggtg gctgcagtga ggaatattgg tcaatggacg taagtctgaa ccagccaagt 60
cgcgtgaggg aagactgccc tatgggttgt aaacctcttt tataagggaa gaataagttc 120
tacgtgtaga atgatgcctg taccttatga ataagcatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatgc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240
cggtttatta agttagtggt taaatatttg agctaaactc aattgtgcca ttaatactgg 300
taaactggag tacagacgag gtaggcggaa taagttaagt agcggtgaaa tgcatagata 360
taacttagaa ctccgatagc gaaggcagct taccagactg taactgacgc tgatgcacga 420
gagcgtgggt agcgaacagg attagatacc ctagtagtc 459
<210> 59
<211> 440
<212> DNA
<213> OTU703
<400> 59
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggagaaca agttggaagt 240
gaaatccatg ggctcaaccc atgaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ctgaggcacg aaggccaggg gagcgaaagg 420
gattagatac cccagtagtc 440
<210> 60
<211> 441
<212> DNA
<213> OTU707
<400> 60
cctacgggag gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaatgacg 120
gtacctgact aagaagctcc ggctaaatac gtgccagcag ccgcggtaat acgtatggag 180
caagcgttat ccggaattac tgggtgtaaa gggtgcgtag gtggtatggc aagtcagaag 240
tgaaaaccca gggcttaact ctgggactgc ttttgaaact gtggaactgg agtgcaggag 300
aggtaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg aacaccagtg 360
gcgaaggcgg cttactggac ggtaactgac gttgaggctc gaaagcgtgg ggagcaaaca 420
ggattagata ccctagtagt c 441
<210> 61
<211> 460
<212> DNA
<213> OTU717
<400> 61
cctacgggag gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgaaggctc tatgggtcgt aaacttcttt tatatgggaa taaagtattc 120
cacgtgtgga attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300
gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactagact gtcactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac ccctgtagtc 460
<210> 62
<211> 460
<212> DNA
<213> OTU724
<400> 62
cctacgggag gctgcagtga ggaatattgg tcaatggacg caagtctgaa ccagccatgc 60
cgcgtgcagg aagacggctc tatgagttgt aaactgcttt tgtacgaggg taaacgctct 120
tacgtgtaag agcctgaaag tatcgtacga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggattc aagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240
cggtttgata agttagaggt gaaatttcgg ggctcaaccc tgaacgtgcc tctaatactg 300
ttgagctaga gagtagttgc ggtaggcgga atgtatggtg tagcggtgaa atgcttagag 360
atcatacaga acaccgattg cgaaggcagc ttaccaaact atatctgacg ttgaggcacg 420
aaagcgtggg gagcaaacag gattagatac cccagtagtc 460
<210> 63
<211> 440
<212> DNA
<213> OTU736
<400> 63
cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg tggcaaggca agccagaagt 240
gaaaacccgg ggctcaaccg cgggattgct tttggaactg tcatgctaga gtgcaggagg 300
ggtgagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggagg 360
cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 64
<211> 440
<212> DNA
<213> OTU752
<400> 64
cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120
tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggctgtgca agtctgaagt 240
gaaaggcatg ggctcaacct gtggactgct ttggaaactg tgcagctaga gtgtcggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttgctggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 65
<211> 442
<212> DNA
<213> OTU757
<400> 65
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaaatgac 120
ggtacctgac taagaagcac cggctaaata cgtgccagca gccgcggtaa tacgtaggtg 180
gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggcggacttt taagtgagat 240
gtgaaatacc cgggctcaac ttgggtgctg catttcaaac tggaagtcta gagtgcagga 300
gaggagaatg gaattcctag tgtagcggtg aaatgcgtag agattaggaa gaacaccagt 360
ggcgaaggcg attctctgga ctgtaactga cgctgaggct cgaaagcgtg gggagcaaac 420
aggattagat accccagtag tc 442
<210> 66
<211> 465
<212> DNA
<213> OTU763
<400> 66
cctacgggtg gcagcagtag ggaatcttcc acaatgggcg aaagcctgat ggagcaacgc 60
cgcgtgtgtg atgaagggtt tcggctcgta aaacactgtt gtaagagaag aatgacattg 120
agagtaactg ttcaatgtgt gacggtatct taccagaaag gaacggctaa atacgtgcca 180
gcagccgcgg taatacgtat gttccaagcg ttatccggat ttattgggcg taaagcgagc 240
gcagacggtt atttaagtct gaagtgaaag ccctcagctc aactgaggaa ttgctttgga 300
aactggatga cttgagtgca gtagaggaaa gtggaactcc atgtgtagcg gtgaaatgcg 360
tagatatatg gaagaacacc agtggcgaag gcggctttct ggactgtaac tgacgttgag 420
gctcgaaagt gtgggtagca aacaggatta gataccctag tagtc 465
<210> 67
<211> 460
<212> DNA
<213> OTU771
<400> 67
cctacgggtg gctgcagtga ggaatattgg tcaatggacg caagtctgaa ccagccatgc 60
cgcgtgcagg aagacggctc tatgagttgt aaactgcttt tgtactaggg taaacgcttc 120
tacgtgtagg agcctgaaag tatagtacga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300
gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactagact gcaactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cccagtagtc 460
<210> 68
<211> 440
<212> DNA
<213> OTU774
<400> 68
cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgtagg tggcaaggca agccagaagt 240
gaaaacccgg ggctcaaccg cgggattgct tttggaactg tcatgctaga gtgcaggagg 300
ggtgagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggagg 360
cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 69
<211> 444
<212> DNA
<213> OTU777
<400> 69
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgaagg aagaaggttt tcggatcgta aacttctatc aacagggacg aagaaagtga 120
cggtacctga ataagaagcc ccggctaact acgtgccagc agccgcggta atacgtaggg 180
ggcaagcgtt atccggaatt actgggtgta aagggagcgt aggcggcacg ccaagccaga 240
tgtgaaagcc cgaggcttaa cctcgcggat tgcatttgga actggcgagc tagagtacag 300
gagaggaaag cggaattcct agtgtagcgg tgaaatgcgt agatattagg aagaacacca 360
gtggcgaagg cggctttctg gactgaaact gacgctgagg ctcgaaagcg tggggagcaa 420
acaggattag ataccctagt agtc 444
<210> 70
<211> 439
<212> DNA
<213> OTU780
<400> 70
cctacgggtg gcagcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcaacgc 60
cgcgtgagcg atgaaggcct tcgggtcgta aagctctgtc ctcaaggaag ataatgacgg 120
tacttgagga ggaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
tagcgttatc cggatttact gggcgtaaag ggtgcgtagg cggtctttta agtcaggagt 240
gaaaggctac ggctcaaccg tagtaagctc ttgaaactgg aggacttgag tgcaggagag 300
gagagtggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaggaa caccagtagc 360
gaaggcggct ctctggactg taactgacgc tgaggcacga aagcgtgggg agcaaacagg 420
attagatacc ctagtagtc 439
<210> 71
<211> 440
<212> DNA
<213> OTU783
<400> 71
cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag acagtgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggtatcaca agtcagaagt 240
gaaagcccgg ggctcaaccc cgggactgct tttgaaactg tggaactgga gtgcaggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 72
<211> 440
<212> DNA
<213> OTU787
<400> 72
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgcggca agtcagatgt 240
gaaaacccgg ggctcaaccc cgggactgca tttgaaactg tcggactaga gtgccggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 73
<211> 460
<212> DNA
<213> OTU793
<400> 73
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgaccgccc tatgggttgt aaacttcttt tatatgggaa taaagggtgc 120
cacgtgtggc attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggacatgta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300
cgtgtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cccagtagtc 460
<210> 74
<211> 440
<212> DNA
<213> OTU801
<400> 74
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgaggca agtctgatgt 240
gaaaacccgg ggctcaaccc cgtgactgca ttggaaactg ttttgcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gcaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 75
<211> 440
<212> DNA
<213> OTU828
<400> 75
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaatccatg ggctcaaccc atgaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 76
<211> 440
<212> DNA
<213> OTU844
<400> 76
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtc ccaggggacg ataatgacgg 120
taccctggga ggaagcaccg gctaactacg tgccagcagc cgcggtaaaa cgtagggtgc 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggattggca agttgggagt 240
gaaatctatg ggctcaaccc ataaattgct ttcaaaactg tcagtcttga gtggtgtaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ctactgggca ctaactgacg ctgaggctcg aaagcatggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 77
<211> 440
<212> DNA
<213> OTU845
<400> 77
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg tggcaaggca agccagaagt 240
gaaaacccgg ggctcaaccg cgggattgct tttggaactg tcatgctaga gtgcaggagg 300
ggtgagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggagg 360
cgaaggcgat tctctggact gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccctgtagtc 440
<210> 78
<211> 442
<212> DNA
<213> OTU846
<400> 78
cctacgggtg gctgcagtga ggaatcttcc acaatgggcg aaagcctgat ggagcaacgc 60
cgcgtgaagg atgaaggcct tcgggttgta aacttctttt atgagtgaag aatatgacgg 120
taactcatga ataagcaccg gctaactacg tgccagcagc cgcggtcata cgtagggtgc 180
aagcattatc cggagtgact gggcgtaaag agttgcgtag gcggtttaat aagtgaatag 240
tgaaacctgg tggctcaacc atacagacta ttattcaaac tgttaaactc gagaatggta 300
gaggtaactg gaatttctag tgtaggagtg aaatccgtag atattagaag gaacaccgat 360
ggcgtaggca ggttactggg ccatttctga cgctaaggca cgaaagcgtg gggagcgaac 420
cggattagat acccgagtag tc 442
<210> 79
<211> 440
<212> DNA
<213> OTU850
<400> 79
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggcagtgca agtcagatgt 240
gaaaggccgg ggctcaaccc cggagctgca tttgaaactg ctcggctaga gtacaggaga 300
ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 80
<211> 440
<212> DNA
<213> OTU866
<400> 80
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggaaggcta agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg gtcatctaga gtgtcggagg 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 81
<211> 460
<212> DNA
<213> OTU870
<400> 81
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg taagcctgaa ccagccaagt 60
cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgtgg 120
gacgtgtcct attttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgagga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 82
<211> 440
<212> DNA
<213> OTU873
<400> 82
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaagtgacgg 120
tacctgacta agaagccccg gctaattacg tgccagcagc cgcggtaata cgtaaggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtttggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcagacttga gtgccggaga 300
ggcaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctgctggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctggtagtc 440
<210> 83
<211> 440
<212> DNA
<213> OTU875
<400> 83
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg atgaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactccg tgccagcagc cgcggtaata cggaggatcc 180
gagcgttatc cggatttatt gggtttaaag ggagcgtagg tggattgtta agtcagttgt 240
gaaagtttgc ggctcaaccg taaaattgca gttgaaactg gcagtcttga gtacagtaga 300
ggtgggcgga attcgtggtg tagcggtgaa atgcttggat atcacgaaga actccgattg 360
cgaaggcagc tcactagact gcaactgaca ctgatgctcg aaagtgtggg tatcaaacag 420
gattagatac ccgagtagtc 440
<210> 84
<211> 440
<212> DNA
<213> OTU893
<400> 84
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcagcgca agtctgaagt 240
gaaagcccgg ggctcaaccc cggaatggct ttggaaactg tgcagctaga gtaccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 85
<211> 460
<212> DNA
<213> OTU944
<400> 85
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggactggta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
tcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420
aaagtgtggg tatcaaacag gattagatac ccttgtagtc 460
<210> 86
<211> 460
<212> DNA
<213> OTU948
<400> 86
cctacgggag gcagcagtga ggaatattgg tcaatgggcg taagcctgaa ccagccaagt 60
cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgtgg 120
gacgtgtcct attttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240
cggcctttta agtcagcggt gaaagtctgt ggctcaacca tagaattgcc gttgaaactg 300
ggaggcttga gtatgtttga ggcaggcgga atgcgtggtg tagcggtgaa atgcttagat 360
atcacgcaga accccgattg cgaaggcagc ctgccaagcc atgactgacg ctgatgcacg 420
aaagcgtggg gatcaaacag gattagatac ccctgtagtc 460
<210> 87
<211> 442
<212> DNA
<213> OTU949
<400> 87
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaaatgac 120
ggtacctgac taagaagcac cggctaaata cgtgccagca gccgcggtaa tacgtatggt 180
gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggtgtgg caagtctgat 240
gtgaaaggca tgggctcaac ctgtggactg cattggaaac tgtcatactt gagtgccgga 300
ggggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360
ggcgaaggcg gcttactgga cgataactga cgctgaggct cgaaagcgtg gggagcaaac 420
aggattagat accctagtag tc 442
<210> 88
<211> 465
<212> DNA
<213> OTU962
<400> 88
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtgtg aagaaggcct tcgggttgta aagcactttc agcggggagg aaggcggtga 120
ggttaataac ctcatcgatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtc tgtcaagtcg gatgtgaaat ccccgggctc aacctgggaa ctgcattcga 300
aactggcagg ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacaaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gataccctag tagtc 465
<210> 89
<211> 440
<212> DNA
<213> OTU983
<400> 89
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240
gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctaga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 90
<211> 460
<212> DNA
<213> OTU1002
<400> 90
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg atggcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttact gggtgtaaag ggagcgcaga 240
cggcactgca agtctgaagt gaaagcccgg ggctcaaccc cgggactgct ttggaaactg 300
tagagctaga gtgctggaga ggcaagcgga attcctagtg tagcggtgaa atgcgtagat 360
attaggaaga acaccagtgg cgaaggcggc ttactggaca gtaactgacg ttgaggctcg 420
aaagcgtggg gagcaaacag gattagatac cctagtagtc 460
<210> 91
<211> 440
<212> DNA
<213> OTU1013
<400> 91
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg atgacggcct tcgggttgta aagctctgtc ttcagggacg ataatgacgg 120
tacctgagga ggaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 180
gagcgttgtc cggatttact gggcgtaaag ggagcgtagg cggactttta agtgagatgt 240
gaaatacccg ggctcaactt gggtgctgca tttcaaactg gaagtctaga gtgcaggaga 300
ggagaatgga attcctagtg tagcggtgaa atgcgtagag attaggaaga acaccagtgg 360
cgaaggcgat tctctggact gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 92
<211> 440
<212> DNA
<213> OTU1024
<400> 92
cctacgggcg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240
gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctaga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 93
<211> 440
<212> DNA
<213> OTU1038
<400> 93
cctacggggg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240
gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggagg 360
cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 94
<211> 465
<212> DNA
<213> OTU1053
<400> 94
cctacgggag gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa 120
agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtc tgtcaagtcg gatgtgaaat ccccgggctc aacctgggaa ctgcattcga 300
aactggcagg ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaagacc ggtggcgaag gcggccccct ggacgaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gatacccgtg tagtc 465
<210> 95
<211> 442
<212> DNA
<213> OTU1064
<400> 95
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgaagg aagaaggctt tcgggttgta aacttctttt gtcagggacg agcagaagac 120
ggtacctgac gaataagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180
gcaagcgttg tccggattta ctgggtgtaa agggcgtgta gccgggtcgg caagtcagat 240
gtgaaatcca cgggcttaac tcgtgaactg catttgaaac tgttgatctt gagtatcgga 300
gaggcaatcg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360
ggcgaaggcg gattgctgga cgacaactga cggtgaggcg cgaaagcgtg gggagcaaac 420
aggattagat accccagtag tc 442
<210> 96
<211> 440
<212> DNA
<213> OTU1070
<400> 96
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggcgaagca agtctgaagt 240
gaaaacccag ggctcaaccc tgggactgct ttggaaactg ttttgctaga gtgtcggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 97
<211> 440
<212> DNA
<213> OTU1081
<400> 97
cctacgggag gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggacgggca agtctgatgt 240
gaaagcccgg ggcttaaccc cgggactgca ttggaaactg tccatcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccctgtagtc 440
<210> 98
<211> 440
<212> DNA
<213> OTU1150
<400> 98
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggaagagca agtctgatgt 240
gaaaggctgg ggcttaaccc caggactgca ttggaaactg tttttcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 99
<211> 440
<212> DNA
<213> OTU1157
<400> 99
cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120
tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaaa cgtagggtgc 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaaaccatg ggctcaaccc atgaattgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtagatgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggt ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 100
<211> 440
<212> DNA
<213> OTU1163
<400> 100
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagt aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgatgca agtctgaagt 240
gaaatacccg ggctcaacct gggaactgct ttggaaactg tatggctaga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttactggaca gtaactgacg ttcaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 101
<211> 460
<212> DNA
<213> OTU1170
<400> 101
cctacgggtg gctgcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagc 120
cacgtgtggc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cgggttgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gcgaccttga gtgcaacaga ggtaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ttactggatt gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 102
<211> 460
<212> DNA
<213> OTU1188
<400> 102
cctacgggag gcagcagtga ggaatattgg tcaatgggcg caggcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatgtatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240
cggtttgata agttagaggt gaaatcccgg ggcttaactc cggaactgcc tctaatactg 300
ttagactaga gagtagttgc ggtaggcgga atgtatggtg tagcggtgaa atgcttagag 360
atcatacaga acaccgattg cgaaggcagc ttaccaaact atatctgacg ttgaggcacg 420
aaagcgtggg gagcaaacag gattagatac cccagtagtc 460
<210> 103
<211> 440
<212> DNA
<213> OTU1205
<400> 103
cctacgggag gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagcaacgc 60
cgcgtgagtg atgacggcct tcgggttgta aagctctgtc ttcagggacg ataatgacgg 120
tacctgagga ggaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 180
gagcgttgtc cggatttact gggcgtaaag ggagcgtagg cggattttta agtgagatgt 240
gaaatactcg ggcttaacct gagtgctgca tttcaaactg gaagtctaga gtgcaggaga 300
ggagaaggga attcctagtg tagcggtgaa atgcgtagag attaggaaga acaccagtgg 360
cgaaggcgct tctctggact gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccctgtagtc 440
<210> 104
<211> 445
<212> DNA
<213> OTU1207
<400> 104
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagcgacgc 60
cgcgtgaggg atggaggcct tcgggttgta aacctctttt atcggggagc aagcgagagt 120
gagtttaccc gttgaataag caccggctaa ctacgtgcca gcagccgcgg taatacgtag 180
ggtgcaagcg ttatccggaa ttattgggcg taaagggctc gtaggcggtt cgtcgcgtcc 240
ggtgtgaaag tccatcgctt aacggtggat ccgcgccggg tacgggcggg cttgagtgcg 300
gtaggggaga ctggaattcc cggtgtaacg gtggaatgtg tagatatcgg gaagaacacc 360
aatggcgaag gcaggtctct gggccgttac tgacgctgag gagcgaaagc gtggggagcg 420
aacaggatta gataccctag tagtc 445
<210> 105
<211> 440
<212> DNA
<213> OTU1208
<400> 105
cctacgggtg gcagcagtag ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatt tcggtatgta aacttctatc agcaaggaag aaaatgacgg 120
tacttgacta agaagccccg gctaaatacg tgccagcagc cgcggtaata cgtatggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggaaggcta agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg gtcatctaga gtgtcggagg 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg atgactgacg ttgaggcttg aaagcgtggg gagcaaacag 420
gattagatac cctggtagtc 440
<210> 106
<211> 443
<212> DNA
<213> OTU1271
<400> 106
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgaagg aagaagtatt tcggtatgta aacttctatc gacagggaag aaacaaatga 120
cggtacctga ataagaagca ccggctaaat acgtgccagc agccgcggta atacgtatgg 180
tgcaagcgtt atccggattt actgggtgta aagggtgagt aggcggtcat gcaagtcata 240
tgtgaaatgt cagggcttaa ccttggcgct gcataagaaa ctgtatgact agagtgcagg 300
agaggtaagc ggaattccta gtgtagcggt gaaatgcgta gatattagga agaacaccgg 360
tggcgaaggc ggcttactgg actgttactg acgctgagtc acgaaagcgt ggggagcaaa 420
caggattaga taccctagta gtc 443
<210> 107
<211> 440
<212> DNA
<213> OTU1274
<400> 107
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cacggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggctgtgta agtctgaagt 240
gaaagcccgg ggctcaaccc cgggactgct ttggaaacta tgcagctaga gtgtcggaga 300
ggtaagtgga attcccagtg tagcggtgaa atgcgtagat attgggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 108
<211> 460
<212> DNA
<213> OTU1276
<400> 108
cctacgggcg gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120
cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240
cggcctttta agtcagcggt gaaagtctgt ggctcaacca tagaattgcc gttgaaactg 300
gggggcttga gtatgtttga ggcaggcgga atgcgtggtg tagcggtgaa atgcttagat 360
atcacgcaga accccgattg cgaaggcagc ctgccaagcc atgactgacg ctgatgcacg 420
aaagcgtggg gatcaaacag gattagatac cctggtagtc 460
<210> 109
<211> 465
<212> DNA
<213> OTU1298
<400> 109
cctacgggtg gctgcagtag ggaatcttcg gcaatggggg caaccctgac cgagcaacgc 60
cgcgtgagtg aagaaggttt tcggatcgta aagctctgtt gtaagagaag aacgtgtgtg 120
agagtggaaa gttcacacag tgacggtaac ttaccagaaa gggacggcta actacgtgcc 180
agcagccgcg gtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag 240
cgcaggcggt ttaataagtc tgaagttaaa ggcagtggct taaccattgt tcgctttgga 300
aactgttaga cttgagtgca gaaggggaga gtggaattcc atgtgtagcg gtgaaatgcg 360
tagatatatg gaggaacacc ggtggcgaaa gcggctctct ggtctgtaac tgacgctgag 420
gctcgaaagc gtggggagca aacaggatta gataccccag tagtc 465
<210> 110
<211> 442
<212> DNA
<213> OTU1359
<400> 110
cctacgggtg gcagcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60
cgcgtgaagg aagaaggctt tcgggttgta aacttctttt aagtgggaag agtagaagac 120
ggtaccactt gaataagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180
gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccgggcatg caagtcagat 240
gtgaaatctc agggcttaac cctgaaactg catttgaaac tgtatgtctt gagtgccgga 300
gaggtaatcg gaattccttg tgtagcggtg aaatgcgtag atataaggaa gaacaccagt 360
ggcgaaggcg gattactgga cggtaactga cggtgaggcg cgaaagcgtg gggagcgaac 420
aggattagat accctagtag tc 442
<210> 111
<211> 440
<212> DNA
<213> OTU1377
<400> 111
cctacgggag gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggcacagca agtcagaagt 240
gaaagcccgg ggctcaaccc cgggactgct tttgaaactg ttgtgctgga gtgcaggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagcgg 360
cgaaggcggc ttactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgggtagtc 440
<210> 112
<211> 460
<212> DNA
<213> OTU1404
<400> 112
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgaaggctc tatgggtcgt aaacttcttt tatatgggaa taaagttttc 120
cacgtgtgga attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactagact gttactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 113
<211> 441
<212> DNA
<213> OTU1412
<400> 113
cctacgggtg gctgcagtgg ggaatcttgc gcaatggggg gaaccctgac gcagcgacgc 60
cgcgtgcggg acggaggcct tcgggtcgta aaccgctttc agcagggaag agtcaagact 120
gtacctgcag aagaagcccc ggctaactac gtgccagcag ccgcggtaat acgtaggggg 180
cgagcgttat ccggattcat tgggcgtaaa gcgcgcgtag gcggcccggc aggccggggg 240
tcgaagcggg gggctcaacc ccccgaagcc cccggaacct ccgcggcttg ggtccggtag 300
gggagggtgg aacacccggt gtagcggtgg aatgcgcaga tatcgggtgg aacaccggtg 360
gcgaaggcgg ccctctgggc cgagaccgac gctgaggcgc gaaagctggg ggagcgaaca 420
ggattagata ccctagtagt c 441
<210> 114
<211> 440
<212> DNA
<213> OTU1413
<400> 114
cctacgggcg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttggggaag ataatgacgg 120
tacccaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaatctatg ggctcaaccc ataaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 115
<211> 441
<212> DNA
<213> OTU1420
<400> 115
cctacgggtg gctgcagtgg gggatattgc gcaatggggg caaccctgac gcagcaacgc 60
cgcgtgaagg atgaaggttt tcggattgta aacttctttt attaaggacg aaaaatgacg 120
gtacttaatg aataagctcc ggctaactac gtgccagcag ccgcggtaat acgtaggggg 180
caagcgttat ccggatttac tgggtgtaaa gggagcgtag acggtgtggc aagtctgatg 240
tgaaaggcat gggctcaacc tgtggactgc attggaaact gtcatacttg agtgccggag 300
gggtaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg aacaccagtg 360
gcgaaggcgg cttactggac ggtaactgac gttgaggctc gaaagcgtgg ggagcaaaca 420
ggattagata ccccagtagt c 441
<210> 116
<211> 440
<212> DNA
<213> OTU1444
<400> 116
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg atgaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggttatgta agtctgatgt 240
gaaaacccgg ggctcaaccc cgggactgca ttggaaacta tgtaactaga gtgtcggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg atgactgacg gtgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 117
<211> 440
<212> DNA
<213> OTU1446
<400> 117
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240
gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggt ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420
gattagatac ccgtgtagtc 440
<210> 118
<211> 440
<212> DNA
<213> OTU1450
<400> 118
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtacggca agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcggactaga gtgtcggagg 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 119
<211> 465
<212> DNA
<213> OTU1464
<400> 119
cctacgggtg gctgcagtag ggaatcttcc acaatggacg caagtctgat ggagcaacgc 60
cgcgtgagtg aagaaggttt tcggatcgta aagctctgtt gttggtgaag aaggatagag 120
gtagtaactg gcctttattt gacggtaatc aaccagaaag tcacggctaa ctacgtgcca 180
gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc 240
gcaggcggaa gaataagtct gatgtgaaag ccctcggctt aaccgaggaa ctgcatcgga 300
aactgttttt cttgagtgca gaagaggaga gtggaactcc atgtgtagcg gtggaatgcg 360
tagatatatg gaagaacacc agtggcgaag gcggctctct ggtctgcaac tgacgctgag 420
gctcgaaagc atgggtagcg aacaggatta gataccctag tagtc 465
<210> 120
<211> 440
<212> DNA
<213> OTU1467
<400> 120
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240
gaaagcccgg ggctcaaccc cgggactgca ttggaaactg tcgtacttga gtatcggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420
gattagatac ccttgtagtc 440
<210> 121
<211> 440
<212> DNA
<213> OTU1476
<400> 121
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg atgaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggcatggca agtcagaagt 240
gaaagcctgg ggctcaaccc cggaattgct tttgaaactg tcaggctaga gtgtcggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccctgtagtc 440
<210> 122
<211> 465
<212> DNA
<213> OTU1488
<400> 122
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtgtg aagaaggcct tcgggttgta aagcactttc agcggggagg aaggcgttaa 120
ggttaataac cttggcgatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcgagcg ttaatcggaa taactgggcg taaagggcac 240
gcaggcggtg acttaagtga ggtgtgaaag ccccgggctc aacctgggaa ctgcatctga 300
tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gatacccgtg tagtc 465
<210> 123
<211> 440
<212> DNA
<213> OTU1502
<400> 123
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg atgaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggagtggca agtctgatgt 240
gaaaacccgg ggctcaaccc cgggactgca ttggaaactg tcaatctaga gtaccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 124
<211> 449
<212> DNA
<213> OTU1503
<400> 124
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgcggg atggaggcct tcgggttgta aaccgctttt gatcgggagc aagcccttcg 120
gggtgagtgt acctttcgaa taagcaccgg ctaactacgt gccagcagcc gcggtaatac 180
gtagggtgca agcgttatcc ggaattattg ggcgtaaagg gctcgtaggc ggttcgtcgc 240
gtccggtgtg aaagcccatc gcttaacggt gggtctgcgc cgggtacggg cgggctggag 300
tgcggtaggg gagactggaa ttcccggtgt aacggtggaa tgtgtagata tcgggaagaa 360
caccaatggc gaaggcaggt ctctgggccg tcactgacgc tgaggagcga aagcgtgggg 420
agcgaacagg attagatacc cttgtagtc 449
<210> 125
<211> 440
<212> DNA
<213> OTU1518
<400> 125
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag ataatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggcggagca agtcagaagt 240
gaaagcccgg ggctcaaccc cgggacggct tttgaaactg ccctgcttga tttcaggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggact gacaatgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 126
<211> 440
<212> DNA
<213> OTU1522
<400> 126
cctacgggtg gcagcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240
gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 127
<211> 459
<212> DNA
<213> OTU1538
<400> 127
cctacggggg ctgcagtgag gaatattggt caatgggcgc aggcctgaac cagccaagta 60
gcgtgaagga tgactgccct atgggttgta aacttctttt ataaaggaat aaagtcgggt 120
atgtataccc gtttgcatgt actttatgaa taaggatcgg ctaactacgt gccagcagcc 180
gcggtaatac gtagggggca agcgttatcc ggatttactg ggtgtaaagg gagcgtagac 240
ggtgtggcaa gtctgatgtg aaaggcatgg gctcaacctg tggactgcat tggaaactgt 300
catacttgag tgccggaggg gtaagcggaa ttcctagtgt agcggtgaaa tgcgtagata 360
ttaggaagaa caccagtggc gaaggcggct tactggacag taactgacgt tgaggctcga 420
aagcgtgggg agcaaacagg attagatacc ccagtagtc 459
<210> 128
<211> 440
<212> DNA
<213> OTU1556
<400> 128
cctacgggag gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggcagtgca agtcagatgt 240
gaaaggccgg ggctcaaccc cggagctgca tttgaaactg ctcggctaga gtacaggaga 300
ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 129
<211> 460
<212> DNA
<213> OTU1563
<400> 129
cctacgggtg gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120
cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cggggtatta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gtatccttga gtgcagcaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggagt gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac ccgtgtagtc 460
<210> 130
<211> 440
<212> DNA
<213> OTU1564
<400> 130
cctacgggag gctgcagtgg gggatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag acagtgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcgtactaga gtgtcggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggact gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 131
<211> 440
<212> DNA
<213> OTU1599
<400> 131
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcaggaaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggttttgca agtctgaagt 240
gaaagcccgg ggcttaaccc cgggactgct ttggaaactg taggactaga gtgcaggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggact gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 132
<211> 460
<212> DNA
<213> OTU1605
<400> 132
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cgtatggtgc aagcgttatc cggatttact gggtgtaaag ggagcgcagg 240
cggtgcggca agtctgatgt gaaagcccgg ggctcaaccc cggtactgca ttggaaactg 300
tcgtactaga gtgtcggagg ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360
attaggagga acaccagtgg cgaaggcggc ttactggacg gtaactgacg ttgaggctcg 420
aaagcgtggg gagcaaacag gattagatac ccgagtagtc 460
<210> 133
<211> 440
<212> DNA
<213> OTU1612
<400> 133
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cggaggatgc 180
gagcgttatc cggatttatt gggtttaaag ggagcgtaga cggatggaca agtctgatgt 240
gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggtgg 360
cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccggtagtc 440
<210> 134
<211> 460
<212> DNA
<213> OTU1626
<400> 134
cctacgggag gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgactgcct tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cgtagggggc aagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cggacgctta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
ggtgtcttga gtacagtaga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 135
<211> 440
<212> DNA
<213> OTU1637
<400> 135
cctacgggcg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggaagagca agtctgatgt 240
gaaaggctgg ggcttaaccc caggactgca ttggaaactg tttttcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgggtagtc 440
<210> 136
<211> 440
<212> DNA
<213> OTU1641
<400> 136
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcaggaaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggcatcaca agtcagaagt 240
gaaagcccgg ggctcaaccc cgggactgct tttgaaactg tggagctgga gtgcaggaga 300
ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 137
<211> 460
<212> DNA
<213> OTU1661
<400> 137
cctacgggtg gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120
cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggagt gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac ccgtgtagtc 460
<210> 138
<211> 460
<212> DNA
<213> OTU1675
<400> 138
cctacgggtg gctgcagtga ggaatattgg tcaatggacg caagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagtattc 120
cacgtgtggg attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 139
<211> 440
<212> DNA
<213> OTU1680
<400> 139
cctacgggag gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtcaagca agtcagaagt 240
gaaaggctgg ggctcaaccc cgggactgct tttgaaactg tttgactgga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 140
<211> 440
<212> DNA
<213> OTU1683
<400> 140
cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120
tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaaa cgtagggtgc 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggaccggca agttggaagt 240
gaaaactatg ggctcaaccc ataaattgct ttcaaaactg ctggccttga gtagtgcaga 300
ggtaggtgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcgac ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420
gattagatac cccagtagtc 440
<210> 141
<211> 440
<212> DNA
<213> OTU1713
<400> 141
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggctttgca agtctgacgt 240
gaaactccgg ggctcaactc cggaactgcg ttggaaactg taaggcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gcaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 142
<211> 440
<212> DNA
<213> OTU1716
<400> 142
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggaattact gggtgtaaag ggagcgtaga cggtaaagca agtctgaagt 240
gaaagcccgg ggctcaaccc cgggactgct ttggaaactg tttaactaga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 143
<211> 440
<212> DNA
<213> OTU1742
<400> 143
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 144
<211> 440
<212> DNA
<213> OTU1758
<400> 144
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggactggca agtctgatgt 240
gaaaggcggg ggctcaaccc ctggactgca ttggaaactg ttagtcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccctgtagtc 440
<210> 145
<211> 440
<212> DNA
<213> OTU1774
<400> 145
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactccg tgccagcagc cgcggtaata cggaggatcc 180
gagcgttatc cggatttatt gggtttaaag ggagcgtaga tgggttgtta agtcagttgt 240
gaaagtttgc ggctcaaccg taaaattgca attgatactg gcagccttga gtacagttga 300
ggtaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360
cgaaggcagc tcactggact gcaactgaca ctgatgctcg aaagtgtggg tatcaaacag 420
gattagatac cccagtagtc 440
<210> 146
<211> 440
<212> DNA
<213> OTU1789
<400> 146
cctacgggtg gcagcagtag ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag acaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggacgggca agtctgaagt 240
gaaaggcagg ggctcaaccc ctggactgct ttggaaactg tccatctaga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgggtagtc 440
<210> 147
<211> 440
<212> DNA
<213> OTU1805
<400> 147
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaatctatg ggcttaaccc ataaactgct ttcaaaactg ctggtcttga gtgatggaga 300
ggcaggcgga attccgtgtg tagcggtgaa atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420
gattagatac ccgagtagtc 440
<210> 148
<211> 443
<212> DNA
<213> OTU1813
<400> 148
cctacgggtg gctgcagtgg ggaatattgc gcaatggggg caaccctgac gcagcaacgc 60
cgcgtgcagg aagaaggtct tcggattgta aactgttgtc gcaagggaag aagacagtga 120
cggtaccttg tgagaaagtc acggctaact acgtgccagc agccgcggta atacgtaggt 180
gacaagcgtt gtccggattt actgggtgta aagggcgcgt aggcggactg tcaagtcagt 240
cgtgaaatac cggggcttaa ccccggggct gcgattgaaa ctgacagcct tgagtatcgg 300
agaggaaagc ggaattccta gtgtagcggt gaaatgcgta gatattagga ggaacaccag 360
tggcgaaggc ggctttctgg acgacaactg acgctgaggc gcgaaagtgt ggggagcaaa 420
caggattaga taccctagta gtc 443
<210> 149
<211> 440
<212> DNA
<213> OTU1824
<400> 149
cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtaat tcgttatgta aagctctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggcatcaca agtcagaagt 240
gaaagcccgg ggctcaaccc cgggactgct tttgaaactg tggagctgga gtgcaggaga 300
ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 150
<211> 440
<212> DNA
<213> OTU1830
<400> 150
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatgggca agtctgatgt 240
gaaaacccgg ggctcaaccc cgggactgca ttggaaactg ttcatctaga gtgctggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc cccctggaca aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 151
<211> 442
<212> DNA
<213> OTU1831
<400> 151
cctacgggtg gctgcagtgg ggaatattgg gcaatggacg caagtctgac ccagcaacgc 60
cgcgtgaagg aagaaggctt tcgggttgta aacttctttt gtcagggaag agtagaagac 120
ggtacctgac gaataagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180
gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccgggccgg caagtcagat 240
gtgaaatctg gaggcttaac ctccaaactg catttgaaac tgtaggtctt gagtaccgga 300
gaggttatcg gaattccttg tgtagcggtg aaatgcgtag atataaggaa gaacaccagt 360
ggcgaaggcg gataactgga cggcaactga cggtgaggcg cgaaagcgtg gggagcaaac 420
aggattagat accctagtag tc 442
<210> 152
<211> 465
<212> DNA
<213> OTU1835
<400> 152
cctacgggag gcagcagtag ggaatcttcg gcaatggggg caaccctgac cgagcaacgc 60
cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa 120
agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtt tgttaagtca gatgtgaaat ccccgggctc aacctgggaa ctgcatctga 300
tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gataccctag tagtc 465
<210> 153
<211> 443
<212> DNA
<213> OTU1841
<400> 153
cctacgggtg gctgcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60
cgcgtgaagg aagaaggctt tcgggttgta aacttctttt atgagggacg aaggaagtga 120
cggtacctca tgaataagct ccggctaact acgtgccagc agccgcggta atacgtaggg 180
agcgagcgtt atccggattt actgggtgta aagggcgtgt aggcggggaa gcaagtcaga 240
tgtgaaaacc agtggctcaa ccactggcct gcatttgaaa ctgtttttct tgagtgatgg 300
agaggcaggc ggaattccgt gtgtagcggt gaaatgcgta gatatacgga ggaacaccag 360
tggcgaaggc ggcctgctgg acattaactg acgctgaggc gcgaaagcgt ggggagcaaa 420
caggattaga taccctagta gtc 443
<210> 154
<211> 440
<212> DNA
<213> OTU1844
<400> 154
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtaaagca agtctgaagt 240
gaaagcccgg ggctcaaccc cgggactgct ttggaaactg tttaactaga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 155
<211> 440
<212> DNA
<213> OTU1848
<400> 155
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggatttact gggtgtaaag ggtgcgtagg tggcagtgca agtcagatgt 240
gaaaggccgg ggctcaaccc cggagctgca tttgaaactg ctcggctaga gtacaggaga 300
ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttgctggaca gtaactgacg ttcaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccttgtagtc 440
<210> 156
<211> 440
<212> DNA
<213> OTU1858
<400> 156
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga tggatgttta agtcagttgt 240
gaaagtttgc ggctcaaccg taaaattgca gttgatactg gatatcttga gtgcagttga 300
ggcaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360
cgaaggcagc acactaatcc gtaactgacg ttcatgctcg aaagtgtggg tatcaaacag 420
gattagatac cccagtagtc 440
<210> 157
<211> 441
<212> DNA
<213> OTU1859
<400> 157
cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaatgacg 120
gtacctgact aagaagcacc ggctaaatac gtgccagcag ccgcggtaaa acgtagggtg 180
caagcgttgt ccggaattac tgggtgtaaa gggagcgtag gtggcaaggc aagccagaag 240
tgaaaacccg gggctcaacc gcgggattgc ttttggaact gtcatgctag agtgcaggag 300
gggtgagcgg aattcctagt gtagcggtga aatgcgtaga tattaggaag aacaccagtg 360
gcgaaggcgg cttactggac ggtaactgac gttgaggctc gaaagcgtgg ggagcaaaca 420
ggattagata cccgggtagt c 441
<210> 158
<211> 440
<212> DNA
<213> OTU1880
<400> 158
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagtgtagg tggccaggca agtcagaagt 240
gaaagcccgg ggctcaaccc cgggactgct tttgaaactg cagggctaga gtgcaggagg 300
ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccttgtagtc 440
<210> 159
<211> 465
<212> DNA
<213> OTU1909
<400> 159
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa 120
agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtt tgttaagtca gatgtgaaat ccccgggctc aacctgggaa ctgcatctga 300
tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gataccctag tagtc 465
<210> 160
<211> 460
<212> DNA
<213> OTU1918
<400> 160
cctacgggtg gctgcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120
cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cggacgctta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
ggtgtcttga gtacagtaga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ttgctggact gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 161
<211> 440
<212> DNA
<213> OTU1919
<400> 161
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggaaggcta agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg gtcatctaga gtgtcggagg 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctggtagtc 440
<210> 162
<211> 440
<212> DNA
<213> OTU1936
<400> 162
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 163
<211> 465
<212> DNA
<213> OTU1951
<400> 163
cctacgggtg gctgcagtgg ggaatcttcc gcaatggacg aaagtctgac ggagcaacgc 60
cgcgtgagtg atgacggcct tcgggttgta aagctctgtg atcggggacg aatggctggt 120
atgctaatac catatcagag tgacggtacc cgaatagcaa gccacggcta actacgtgcc 180
agcagccgcg gtaatacgta ggtggcaagc gttgtccgga attattgggc gtaaagcgcg 240
cgcaggcggc ttcttaagtc catcttaaaa gtgcggggct taaccccgtg atgggatgga 300
aactgggagg ctggagtatc ggagaggaaa gtggaattcc tagtgtagcg gtgaaatgcg 360
tagagattag gaagaacacc ggtggcgaag gcgactttct ggacgacaac tgacgctgag 420
gcgcgaaagc gtggggagca aacaggatta gataccctag tagtc 465
<210> 164
<211> 440
<212> DNA
<213> OTU1972
<400> 164
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggtgc 180
aagcgttaat cggaattact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 165
<211> 440
<212> DNA
<213> OTU1977
<400> 165
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaatccatg ggctcaaccc atgaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 166
<211> 440
<212> DNA
<213> OTU1979
<400> 166
cctacgggtg gctgcagtgg ggaattttgc gcaatggggg aaaccctgac gcagcaacgc 60
cgcgtgcggg acgacggcct tcgggttgta aaccgctttc agcagggaag aaattcgacg 120
gtacctgcag aagaagctcc ggctaactac gtgccagcag ccgcggtaat acgtagggag 180
cgagcgttat ccggattcat tgggcgtaaa gagcgcgtag gcggcctctc aagcgggatc 240
tctaatccga gggctcaacc cccggccgga tcccgaactg ggaggctcga gttcggtaga 300
ggcaggcgga attcccggtg tagcggtgga atgcgcagat atcgggaaga acaccgatgg 360
cgaaggcagc ctgctgggcc gcaactgacg ctgaggcgcg aaagctaggg gagcgaacag 420
gattagatac cctagtagtc 440
<210> 167
<211> 460
<212> DNA
<213> OTU2006
<400> 167
cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagtattc 120
cacgtgtggg attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300
gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ttaccaaact atatctgacg ttgaggcacg 420
aaagcgtggg gagcaaacag gattagatac ccctgtagtc 460
<210> 168
<211> 465
<212> DNA
<213> OTU2048
<400> 168
cctacggggg gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa 120
agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtt gattgagtca gatgtgaaat ccccgggctt aacccgggaa ttgcatctga 300
tactggtcag ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gatacccctg tagtc 465
<210> 169
<211> 440
<212> DNA
<213> OTU2052
<400> 169
cctacgggcg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg tggcagtgca agtcagatgt 240
gaaaggccgg ggctcaaccc cggagctgca tttgaaactg ctcggctaga gtacaggaga 300
ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctgctggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccttgtagtc 440
<210> 170
<211> 440
<212> DNA
<213> OTU2086
<400> 170
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcagcgca agtctgaagt 240
gaaagcccgg ggctcaaccc cggaatggct ttggaaactg tgcagctaga gtaccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 171
<211> 440
<212> DNA
<213> OTU2096
<400> 171
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccttgtagtc 440
<210> 172
<211> 440
<212> DNA
<213> OTU2112
<400> 172
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggtatcaca agtcagaagt 240
gaaagcccgg ggctcaaccc cgggactgct tttgaaactg tggaactgga gtgcaggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 173
<211> 440
<212> DNA
<213> OTU2162
<400> 173
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240
gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 174
<211> 440
<212> DNA
<213> OTU2198
<400> 174
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggcagtgca agtcagatgt 240
gaaaggccgg ggctcaaccc cggagctgca tttgaaactg ctcggctaga gtacaggaga 300
ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ctgctggact gttactgaca ctgaggcacg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 175
<211> 472
<212> DNA
<213> OTU2219
<400> 175
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaaccc cgggactgca tttggaactg tcaggctaga gtgtcggaga 300
ggaaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc tttctggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc catctgtctc tggattagat accctagtag tc 472
<210> 176
<211> 439
<212> DNA
<213> OTU2221
<400> 176
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgatgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggaattatt gggcgtaaag agggagcagg cggcactaag ggtctgtggt 240
gaaagatcga agcttaactt cggtaagcca tggaaaccgt agagctagag tgtgtgagag 300
gatcgtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc 360
gaaggcgacg atctggcgca taactgacgc tgaggctcga aagcgtgggg agcaaacagg 420
attagatacc cgagtagtc 439
<210> 177
<211> 440
<212> DNA
<213> OTU2285
<400> 177
cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtaat tcgttatgta aagctctatc agcagggaag atagtgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggccatgca agtcagaagt 240
gaaaatccgg ggctcaaccc cggaactgct tttgaaactg tgaggctgga gtgcaggagg 300
ggtgagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 178
<211> 440
<212> DNA
<213> OTU2294
<400> 178
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggacgctta agtcagttgt 240
gaaagtttgc ggctcaaccg taaaattgca gttgatactg ggtgtcttga gtacagtaga 300
ggcaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360
cgaaggcagc ctgctggact gtaactgacg ctgatgctcg aaagtgtggg tatcaaacag 420
gattagatac cctagtagtc 440
<210> 179
<211> 439
<212> DNA
<213> OTU2306
<400> 179
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcaacgc 60
cgcgtgagcg atgaaggcct tcgggtcgta aagctctgtc ctcaaggaag ataatgacgg 120
tacttgagga ggaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
tagcgttatc cggaattact gggcgtaaag ggtgcgtagg tggtttctta agtcagaggt 240
gaaaggctac ggctcaaccg tagtaagcct ttgaaactgg gaaacttgag tgcaggagag 300
gagagtggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaggaa caccagttgc 360
gaaggcggct ctctggactg taactgacac tgaggcacga aagcgtgggg agcaaacagg 420
attagatacc ctagtagtc 439
<210> 180
<211> 440
<212> DNA
<213> OTU2312
<400> 180
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag atagtgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggagc 180
aagcgttatc cggatttatt gggtgtaaag ggtgcgcaga cggcgatgca agtctggagt 240
gaaagcccgg ggctcaaccc cgggactgct ttggaaactg tatggctaga gtgctggaga 300
ggcaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttgctggaca gtaactgacg ttcaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgggtagtc 440
<210> 181
<211> 442
<212> DNA
<213> OTU2325
<400> 181
cctacgggtg gctgcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60
cgcgtgaagg aagaaggctt tcgggttgta aacttctttt gtcagggacg agtagaagac 120
ggtacctgac gaataagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180
gcaagcgttg tccggattta ctgggtgtaa agggcgtgta gccgggaggg caagtcagat 240
gtgaaatcca cgggctcaac tcgtgaactg catttgaaac tactcttctt gagtatcgga 300
gaggcaatcg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360
ggcgaaggcg gattgctgga cgacaactga cggtgaggcg cgaaagcgtg gggagcaaac 420
aggattagat accctagtag tc 442
<210> 182
<211> 440
<212> DNA
<213> OTU2334
<400> 182
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg atgaagtatt tcggtatgta aacttctatc ggcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtatggca agtctgatgt 240
gaaaggccag ggctcaaccc tgggactgca ttggaaactg tcgaactaga gtgtcggaga 300
ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttgctggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccttgtagtc 440
<210> 183
<211> 443
<212> DNA
<213> OTU2385
<400> 183
cctacgggtg gctgcagtgg ggaatattgg gcaatggggg aaaccctgac ccagcaacgc 60
cgcgtgaagg aagaaggctt tcgggttgta aacttctttt accagggacg aaggacgtga 120
cggtacctgg agaaaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt 180
ggcaagcgtt gtccggattt actgggtgta aagggcgtgt aggcggagaa gcaagtcaga 240
agtgaaatcc atgggcttaa cccatgaact gcttttgaaa ctgtttccct tgagtatcgg 300
agaggcaggc ggaattccta gtgtagcggt gaaatgcgta gatattagga ggaacaccag 360
tggcgaaggc ggcctgctgg acgacaactg acgctgaggc gcgaaagcgt ggggagcaaa 420
caggattaga taccctagta gtc 443
<210> 184
<211> 440
<212> DNA
<213> OTU2397
<400> 184
cctacgggtg gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt ttggtatgta aagctctatc agcagggaag acagtgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240
gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 185
<211> 440
<212> DNA
<213> OTU2402
<400> 185
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttggggaag ataatgacgg 120
tacccaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggactggca agtctgatgt 240
gaaaggcggg ggctcaaccc ctggactgca ttggaaactg ttagtcttga gtgtcggagg 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccctgtagtc 440
<210> 186
<211> 465
<212> DNA
<213> OTU2407
<400> 186
cctacgggtg gctgcagtgg ggaattttgg acaatgggcg caagcctgat ccagctattc 60
cgcgtgtggg atgaaggccc tcgggttgta aaccactttt gtagagaacg aaaagacatc 120
ttcgaataaa ggatgttgct gacggtactc taagaataag caccggctaa ctacgtgcca 180
gcagccgcgg taatacgtag ggtgcgagcg ttaatcggaa ttactgggcg taaagggtgc 240
gcaggcggtt gagtaagaca gatgtgaaat ccccgagctt aactcgggaa tggcatatgt 300
gactgctcga ctagagtgtg tcagagggag gtggaattcc acgtgtagca gtgaaatgcg 360
tagatatgtg gaagaacacc gatggcgaag gcagcctcct gggacataac tgacgctcag 420
gcacgaaagc gtggggagca aacaggatta gataccctag tagtc 465
<210> 187
<211> 460
<212> DNA
<213> OTU2409
<400> 187
cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagttagc 120
cacgtgtggt tttttgcatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cggggtatta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gtatccttga gtgcagcaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggagt gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 188
<211> 446
<212> DNA
<213> OTU2423
<400> 188
cctacgggtg gctgcagtcg agaatcattc acaatggggg aaaccctgat ggtgcgacgc 60
cgcgtggggg aatgaaggtc ttcggattgt aaacccctgt catgtgggag caaattaaaa 120
agatagtacc acaagaggaa gagacggcta actctgtgcc agcagccgcg gtaatacaga 180
ggtctcaagc gttgttcgga atcactgggc gtaaagcgtg cgtaggctgt ttcgtaagtc 240
gtgtgtgaaa ggcgcgggct caacccgcgg acggcacatg atactgcgag actagagtaa 300
tggaggggga accggaattc tcggtgtagc agtgaaatgc gtagatatcg agaggaacac 360
tcgtggcgaa ggcgggttcc tggacattaa ctgacgctga ggcacgaagg ccaggggagc 420
gaaagggatt agatacccta gtagtc 446
<210> 189
<211> 440
<212> DNA
<213> OTU2443
<400> 189
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcaacgc 60
cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcaga cggcactgca agtctgaagt 240
gaaagcccgg ggctcaaccc cgggactgct ttggaaactg tagagctaga gtgctggaga 300
ggcaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttgctggaca gtaactgacg ttcaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 190
<211> 440
<212> DNA
<213> OTU2445
<400> 190
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag ataatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttactggaca gtaactgacg ttgaggcttg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 191
<211> 465
<212> DNA
<213> OTU2460
<400> 191
cctacgggtg gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcgaggagg aaggtgttgt 120
ggttaataac cgcagcaatt gacgttactc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtc tgtcaagtcg gatgtgaaat ccccgggctc aacctgggaa ctgcatccga 300
aactggcagg ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacaaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gatacccctg tagtc 465
<210> 192
<211> 465
<212> DNA
<213> OTU2471
<400> 192
cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcgaggagg aaggcgttgt 120
ggttaataac cgcaacgatt gacgttactc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtc tgtcaagtcg gatgtgaaat ccccgggctc aacctgggaa ctgcatccga 300
aactggcagg ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcagccatct ggcctaatac tgacgctgag 420
gtacgaaagc atggggagca aacaggatta gataccccag tagtc 465
<210> 193
<211> 460
<212> DNA
<213> OTU2473
<400> 193
cctacggggg gctgcagtga ggaatattgg tcaatgggcg caggcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatgtatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgacg ctgaggctcg 420
aaagtgtggg tagcaaacag gattagatac ccgagtagtc 460
<210> 194
<211> 465
<212> DNA
<213> OTU2499
<400> 194
cctacgggtg gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60
cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcgaggagg aaggcattaa 120
ggttaataac cttagtgatt gacgttactc gcagaagaag caccggctaa ctccgtgcca 180
gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac 240
gcaggcggtc tgttaagtca gatgtgaaat ccccgggctc aacctgggaa ctgcatttga 300
aactggcagg cttgagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360
tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacaaagac tgacgctcag 420
gtgcgaaagc gtggggagca aacaggatta gataccccag tagtc 465
<210> 195
<211> 440
<212> DNA
<213> OTU2514
<400> 195
cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtagt tcgctatgta aagctctatc agcagggaag atagtgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggccaggca agtcagaagt 240
gaaagcccgg ggctcaaccc cgggactgct tttgaaactg cagggctaga gtgcaggagg 300
ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 196
<211> 439
<212> DNA
<213> OTU2531
<400> 196
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggtttctta agtcagaggt 240
gaaaggctac ggctcaaccg tagtaagcct ttgaaactgg gaaacttgag tgcaggagag 300
gagagtggaa ttcctagtgt agcggtgaaa tgcgtagata ttacgaggaa caccagttgc 360
gaaggcggct ctctggacag taactgacgt tcaggctcga aagcgtgggg agcaaacagg 420
attagatacc ctagtagtc 439
<210> 197
<211> 439
<212> DNA
<213> OTU2533
<400> 197
cctacggtgg cagcagtggg gaatattgca caatgggcga aagcctgatg cagcaacgcc 60
gcgtgagtga agaagtatct cggtatgtaa agctctatca gcagggaaga aaatgacggt 120
acctgactaa gaagccccgg ctaactacgt gccagcagcc gcggtaatac gtagggggca 180
agcgttatcc ggatttactg ggtgtaaagg gagcgtagac ggcgcagcaa gtctgatgtg 240
aaaggcaggg gcttaacccc tggactgcat tggaaactgc tgtgcttgag tgccggaggg 300
gtaagcggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaggaa caccagtggc 360
gaaggcggct tgctggacag taactgacgt tcaggctcga aagcgtgggg agcaaacagg 420
attagatacc ccggtagtc 439
<210> 198
<211> 443
<212> DNA
<213> OTU2541
<400> 198
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgaagg atgacggttt tcggattgta aacttctttt cttagtgacg aagacagtga 120
cggtagctaa ggaataagca tcggctaact acgtgccagc agccgcggta atacgtagga 180
tgcaagcgtt atccggattt actgggtgta aagggagcgc aggcgggact gcaagttgga 240
tgtgaaatac cgtggcttaa ccacggaact gcatccaaaa ctgtagttct tgagtgaagt 300
agaggcaagc ggaattccga gtgtagcggt gaaatgcgta gatattcgga ggaacaccag 360
tggcgaaggc ggcttgctgg gctttaactg acgctgaggc tcgaaagtgt ggggagcaaa 420
caggattaga taccctagta gtc 443
<210> 199
<211> 460
<212> DNA
<213> OTU2564
<400> 199
cctacgggtg gcagcagtga ggaatattgg tcaatgggcg caggcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatgtatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cggacgctta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
ggtgtcttga gtacagtaga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420
aaagtgtggg tatcaaacag gattagatac ccgtgtagtc 460
<210> 200
<211> 439
<212> DNA
<213> OTU2568
<400> 200
cctaggtggc tgcagtgggg aatattgcac aatggaggaa actctgatgc agcgacgccg 60
cgtgaaggat gaagtatttc ggtatgtaaa gctctatcag cagggaagaa gaatgacggt 120
acctgactaa gaagcaccgg ctaactacgt gccagcagcc gcggtaatac gtagggggca 180
agcgttatcc ggatttactg ggtgtaaagg gagcgtagac ggtgtggcaa gtctgatgtg 240
aaaggcatgg gctcaacctg tggactgcat tggaaactgt catacttgag tgccggaggg 300
gtaagcggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaagaa caccagtggc 360
gaaggcggct tactggacgg taactgacgt tgaggctcga aagcgtgggg agcaaacagg 420
attagatacc cttgtagtc 439
<210> 201
<211> 440
<212> DNA
<213> OTU2600
<400> 201
cctacgggtg gcagcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120
tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaatctatg ggctcaaccc ataaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcgac ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420
gattagatac ccgtgtagtc 440
<210> 202
<211> 465
<212> DNA
<213> OTU2601
<400> 202
cctacgggtg gcagcagtag ggaatcttcg gcaatggacg aaagtctgac cgagcaacgc 60
cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag aacaaggatg 120
agagtaactg ttcatccctt gacggtatct aaccagaaag ccacggctaa ctacgtgcca 180
gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc 240
gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga 300
aactgggaga cttgagtgca gaagaggaga gtggaattcc atgtgtagcg gtgaaatgcg 360
tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac tgacgctgag 420
gctcgaaagc gtggggagca aacaggatta gataccctag tagtc 465
<210> 203
<211> 440
<212> DNA
<213> OTU2618
<400> 203
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg atgacggcct tcgggttgta aagctctgtc ttcagggacg ataatgacgg 120
tacctgagga ggaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 180
aagcgttgtc cggatttact gggcgtaaag ggagcgtagg cggactttta agtgagatgt 240
gaaatacccg ggctcaactt gggtgctgca tttcaaactg gaagtctaga gtgcaggaga 300
ggagaatgga attcctagtg tagcggtgaa atgcgtagag attaggaaga ataccagtgg 360
cgaaggcgcc tttctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccggtagtc 440
<210> 204
<211> 440
<212> DNA
<213> OTU2626
<400> 204
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtaat tcgttatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggccgtgca agtctgatgt 240
gaaaggctgg ggctcaaccc cgggactgca ttggaaactg tatggctgga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctggtagtc 440
<210> 205
<211> 440
<212> DNA
<213> OTU2637
<400> 205
cctacgggtg gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtagt tcgctatgta aagctctatc agcagggaag ataatgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaatccatg ggctcaaccc atgaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgtgtagtc 440
<210> 206
<211> 460
<212> DNA
<213> OTU2647
<400> 206
cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120
cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtgtaaag ggagcgcagg 240
cggtacggca agtctgatgt gaaagcccgg ggctcaaccc cggtactgca ttggaaactg 300
tcggactaga gtgtcggagg ggtaagtgga attcctagtg tagcggtgaa atgcgtagat 360
attaggagga acaccagtgg cgaaggcggc ttactggacg gtaactgacg ttgaggctcg 420
aaagcgtggg gagcaaacag gattagatac cccagtagtc 460
<210> 207
<211> 440
<212> DNA
<213> OTU2686
<400> 207
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgagta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggt caactggacg ataactgacg ctgaggcgcg aaagcgtggg gagcaaacag 420
gattagatac ccgtgtagtc 440
<210> 208
<211> 460
<212> DNA
<213> OTU2693
<400> 208
cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagc 120
cacgtgtggc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ttactggatt gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 209
<211> 440
<212> DNA
<213> OTU2695
<400> 209
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcaggaaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagtgcagg cgggaagaca agttggaagt 240
gaaatccatg ggctcaaccc atgaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ttactggact gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 210
<211> 440
<212> DNA
<213> OTU2703
<400> 210
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240
gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 211
<211> 440
<212> DNA
<213> OTU2717
<400> 211
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcggagca agtctgaagt 240
gaaagcccgg ggctcaaccc cgggactgct ttggaaactg ttctgctaga gtgctggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccggtagtc 440
<210> 212
<211> 440
<212> DNA
<213> OTU2721
<400> 212
cctacgggtg gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag acagtgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcaga cggcactgca agtctgaagt 240
gaaagcccgg ggctcaaccc cgggactgca ttggaaactg tcaatctaga gtaccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 213
<211> 442
<212> DNA
<213> OTU2730
<400> 213
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaaatgac 120
ggtacctgac taagaagcac cggctaaata cgtgccagca gccgcggtaa tacgtatggt 180
gcaagcgtta tccggattta ctgggtgtaa agggagcgca ggcggaaggc taagtctgat 240
gtgaaagccc ggggctcaac cccggtactg cattggaaac tggtcatcta gagtgtcgga 300
ggggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360
ggcgaaggcg gcttactgga cgataactga cgctgaggct cgaaagcgtg gggagcaaac 420
aggattagat accctagtag tc 442
<210> 214
<211> 460
<212> DNA
<213> OTU2734
<400> 214
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg caggcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagttttc 120
cacgtgtgga attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggacagtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gctgtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 215
<211> 440
<212> DNA
<213> OTU2735
<400> 215
cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgtggg aagaaggttt tcggattgta aaccactgtt cttggtgaag ataatgacgg 120
taaccaagga gaaagctccg gctaactacg tgccagcagc cgcggtaata cgtagggagc 180
aagcgttgtc cggatttact gggtgtaaag ggtgcgtagg cggctaggca agtcagacgt 240
gaaaaccatg ggctcaacct gtggactgcg tttgaaactg tttagcttga gtgaagtaga 300
ggtaggcgga attcccggtg tagcggtgaa atgcgtagag atcgggagga acaccagtgg 360
cgaaggcggc ctactgggct ttaactgacg ctgaggcacg aaagcatggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 216
<211> 440
<212> DNA
<213> OTU2736
<400> 216
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggtctgaca agtcagaagt 240
gaaagcccgg ggctcaactc cgggactgct tttgaaactg ccggactaga ttgcaggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggact gtaaatgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 217
<211> 440
<212> DNA
<213> OTU2770
<400> 217
cctacggggg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240
gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctgga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctggtagtc 440
<210> 218
<211> 439
<212> DNA
<213> OTU2813
<400> 218
cctacgggtg gcagcagtgg gggatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcgtactaga gtgtcggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcgac ttactggact gctactgaca ctgaggcacg aaagcgtggg gagcaaacag 420
gattagatac ccagtagtc 439
<210> 219
<211> 440
<212> DNA
<213> OTU2819
<400> 219
cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240
gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctaga gtgctggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 220
<211> 440
<212> DNA
<213> OTU2835
<400> 220
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaatctatg ggctcaaccc ataaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420
gattagatac ccctgtagtc 440
<210> 221
<211> 440
<212> DNA
<213> OTU2851_dkd43_100000030018
<400> 221
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcatgaca agccagatgt 240
gaaaacccag ggctcaaccc tgggactgca tttggaactg ccaggctgga gtgcaggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccttgtagtc 440
<210> 222
<211> 440
<212> DNA
<213> OTU2867
<400> 222
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaagtgacgg 120
tacctgaata agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgaagt 240
gaaagcccgg tgcttaacgc cgggactgct ttggaaactg tttggctgga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 223
<211> 440
<212> DNA
<213> OTU2879_hc56_100000053763
<400> 223
cctacgggtg gctgcagtgg ggaatattgg acaatgggcg caagcctgat ccagcaatgc 60
cgcgtgagtg atgaaggccc tagggttgta aagctctttt acccgggaag ataatgactg 120
taccgggaga ataagccccg gctaactccg tgccagcagc cgcggtaata cggagggggc 180
tagcgttgtt cggaattact gggcgtaaag cgcacgtagg cggctttgta agtcagaggt 240
gaaagcctgg agctcaactc cagaactgcc tttgagactg catcgcttga atccaggaga 300
ggtcagtgga attccgagtg tagaggtgaa attcgtagat attcggaaga acaccagtgg 360
cgaaggcggc tgactggact ggtattgacg ctgaggtgcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 224
<211> 440
<212> DNA
<213> OTU2918
<400> 224
cctacgggtg gcagcagtgg ggaatattgg gcaatggacg caagtctgac ccagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240
gaaatctatg ggctcaaccc ataaactgct ttcaaaactg tttttcttga gtagtgcaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggc ctgctggact gtaactgacg ctgatgctcg aaagtgtggg tatcaaacag 420
gattagatac ccttgtagtc 440
<210> 225
<211> 465
<212> DNA
<213> OTU2933
<400> 225
cctacgggtg gctgcagtgg ggaatcttcc gcaatggacg aaagtctgac ggagcaacgc 60
cgcgtgagtg atgaaggatt tcggtctgta aagctctgtt gtttatgacg aacgtgcagt 120
gtgtgaacaa tgcattgcaa tgacggtagt aaacgaggaa gccacggcta actacgtgcc 180
agcagccgcg gtaatacgta ggtggcgagc gttgtccgga attattgggc gtaaagagca 240
tgtaggcggc ttaataagtc gagcgtgaaa atgcggggct caaccccgta tggcgctgga 300
aactgttagg cttgagtgca ggagaggaaa ggggaattcc cagtgtagcg gtgaaatgcg 360
tagatattgg gaggaacacc agtggcgaag gcgcctttct ggactgtgtc tgacgctgag 420
atgcgaaagc cagggtagcg aacgggatta gataccctag tagtc 465
<210> 226
<211> 443
<212> DNA
<213> OTU2935
<400> 226
cctacgggtg gctgcagtgg ggaatattgc gcaatggggg aaaccctgac gcagcaacgc 60
cgcgtgattg aagaaggcct tcgggttgta aagatcttta atcagggacg aaacaaatga 120
cggtacctga agaataagct ccggctaact acgtgccagc agccgcggta atacgtaggg 180
agcaagcgtt atccggattt actgggtgta aagggcgcgc aggcgggccg gcaagttgga 240
agtgaaatct atgggcttaa cccataaact gctttcaaaa ctgctggtct tgagtgatgg 300
agaggcaggc ggaattccgt gtgtagcggt gaaatgcgta gatatacgga ggaacaccag 360
tggcgaaggc ggcctgctgg acattaactg acgctgaggc gcgaaagcgt ggggagcaaa 420
caggattaga taccctagta gtc 443
<210> 227
<211> 439
<212> DNA
<213> OTU2950
<400> 227
cctagggtgg cagcagtggg gaatattgca caatggggga aaccctgatg cagcgacgcc 60
gcgtggagga agaaggtctt cggattgtaa actcctgttg ttgaggaaga taatgacggt 120
actcaacaag gaagtgacgg ctaactacgt gccagcagcc gcggtaatac ggaggatccg 180
agcgttatcc ggatttattg ggtttaaagg gagcgtaggc ggacgcttaa gtcagttgtg 240
aaagtttgcg gctcaaccgt aaaattgcag ttgatactgg gtgtctcgag tacagtagag 300
gcaggcggaa ttcgtggtgt agcggtgaaa tgcttagata tcacgaagaa ctccgattgc 360
gaaggcagct tgctggactg taactgacgc tgatgctcga aagtgtgggt atcaaacagg 420
attagatacc cgtgtagtc 439
<210> 228
<211> 460
<212> DNA
<213> OTU2954
<400> 228
cctacgggtg gctgcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120
cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactacg tgccagcagc 180
cgcggtaata cgtagggggc aagcgttatc cggatttact gggtgtaaag ggagcgtaga 240
cggcaaggca agtctgatgt gaaaacccag ggcttaaccc tgggactgca ttggaaactg 300
tctggctcga gtgccggaga ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360
attaggagga acaccagtgg cgaaggcgac taactggact gtaactgacg ctgaggcgcg 420
aaagtgtggg gagcaaacag gattagatac cccagtagtc 460
<210> 229
<211> 440
<212> DNA
<213> OTU2955
<400> 229
cctacgggtg gcagcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60
cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtaaagca agtctgaagt 240
gaaagcccgc ggctcaactg cgggactgct ttggaaactg tttaactgga gtgtcggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 230
<211> 440
<212> DNA
<213> OTU2997
<400> 230
cctacgggcg gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgaagg atgaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120
tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggagcagca agtctgatgt 240
gaaaacccgg ggctcaaccc cgggactgca ttggaaactg ttgatctgga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgtgtagtc 440
<210> 231
<211> 440
<212> DNA
<213> OTU3044
<400> 231
cctacgggag gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60
cgcgtgagtg aagaagtagt tcgctatgta aagctctatc agcagggaag atagtgacgg 120
tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180
aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggccaggca agtcagaagt 240
gaaaacccag ggcttaactc tgggactgct tttgaaactg tcagactgga gtgcaggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cctagtagtc 440
<210> 232
<211> 466
<212> DNA
<213> OTU3055
<400> 232
cctacgggtg gctgcagtgg ggaatattgc gcaatgggcg aaagcctgac gcagcgacgc 60
cgcgtgaggg atgaaggttc tcggatcgta aacctctgtc aggggggaag aaaccccctc 120
gtgtgaataa tgcgagggct tgacggtacc cccaaaggaa gcaccggcta actccgtgcc 180
agcagccgcg gtaatacgga gggtgcaagc gttaatcgga atcactgggc gtaaagcgca 240
cgtaggcggc ttggtaagtc aggggtgaaa tcccacagcc caactgtgga actgcctttg 300
atactgccag gcttgagtac cggagagggt ggcggaattc caggtgtagg agtgaaatcc 360
gtagatatct ggaggaacac cggtggcgaa ggcggccacc tggacggtaa ctgacgctga 420
ggtgcgaaag cgtgggtagc aaacaggatt agatacccta gtagtc 466
<210> 233
<211> 440
<212> DNA
<213> OTU3061
<400> 233
cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120
tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaaa cgtagggtgc 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggattggca agttgggagt 240
gaaatctatg ggctcaaccc ataaattgct ttcaaaactg tcagtcttga gtggtgtaga 300
ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360
cgaaggcggt ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420
gattagatac cctagtagtc 440
<210> 234
<211> 440
<212> DNA
<213> OTU3065
<400> 234
cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttggggaag ataatgacgg 120
tacccaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240
gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcgtactaga gtgtcggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac cccagtagtc 440
<210> 235
<211> 439
<212> DNA
<213> OTU3087
<400> 235
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgaagg atgaaggcct ttgggtcgta aacttctgtt ctaagggaag atagtgacgg 120
taccttagga gcaagtcccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
aagcgttatc cggaattatt gggcgtaaag agtacgtagg tggttttcta agcacggggt 240
ttaaggcaat ggcttaacca ttgttcgcct tgtgaactgg aagacttgag tgcaggagag 300
gaaagcggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaggaa caccagtggc 360
gaaggcggct ttctggactg taactgacac tgaggtacga aagcgtgggg agcaaacagg 420
attagatacc ctagtagtc 439
<210> 236
<211> 440
<212> DNA
<213> OTU3094
<400> 236
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttggggaag ataatgacgg 120
tacccaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggtctggca agtctgatgt 240
gaaaatccgg ggctcaactc cggaactgca ttggaaactg tcagactaga gtgtcggaga 300
ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgtgtagtc 440
<210> 237
<211> 460
<212> DNA
<213> OTU3102
<400> 237
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420
aaagtgtggg tatcaaacag gattagatac cctagtagtc 460
<210> 238
<211> 460
<212> DNA
<213> OTU3111
<400> 238
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgaaggctc tatgggtcgt aaacttcttt tatatgggaa taaagttttc 120
cacgtgtgga attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
cggggtatta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gtatccttga gtgcagcata ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactagact gtcactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460
<210> 239
<211> 440
<212> DNA
<213> OTU3112
<400> 239
cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtggagg aagaaggtct tcggtttgta aactcctgtt gttgaggaag ataatgacgg 120
tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180
aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240
gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360
cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccgagtagtc 440
<210> 240
<211> 460
<212> DNA
<213> OTU3128
<400> 240
cctacgggtg gcagcagtga ggaatattgg tcaatggacg caagtctgaa ccagccatgc 60
cgcgtgcagg aagacggctc tatgagttgt aaactgcttt tgtacgagag taaacgctct 120
tacgtgtaag agcctgaaag tatcgtacga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc aagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240
cggtttgata agttagaggt gaaataccgg tgcttaacac cggaactgcc tctaatactg 300
ttgaactaga gagtagttgc ggtaggcgga atgtatggtg tagcggtgaa atgcttagag 360
atcatacaga acaccgattg cgaaggcagc ttaccaaact atatctgacg ttgaggcacg 420
aaagcgtggg gagcaaacag gattagatac cctagtagtc 460
<210> 241
<211> 465
<212> DNA
<213> OTU3131
<400> 241
cctacgggtg gctgcagtag ggaatcttcc acaatggacg caagtctgat ggagcaacgc 60
cgcgtgagtg aagaagggtt tcggctcgta aagctctgtt gttggtgaag aaggacaggg 120
gtagtaactg acctttgttt gacggtaatc aattagaaag tcacggctaa ctacgtgcca 180
gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagt 240
gcaggcggct cgataagtct gatgtgaaag ccttcggctc aaccggagaa ttgcatcaga 300
aactgtcgag cttgagtaca gaagaggaga gtggaactcc atgtgtagcg gtgaaatgcg 360
tagatatatg gaagaacacc ggtggcgaag gcggctctct ggtctgttac tgacgctgag 420
gctcgaaagc atgggtagcg aacaggatta gataccctag tagtc 465
<210> 242
<211> 460
<212> DNA
<213> OTU3137
<400> 242
cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120
tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240
cggtgtggca agtctgatgt gaaaggcatg ggctcaacct gtggactgca ttggaaactg 300
tcatacttga gtgccggagg ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360
attaggagga acaccagtgg cgaaggcggc tttctggacg acaactgacg ctgaggcgcg 420
aaagtgtggg gagcaaacag gattagatac ccgagtagtc 460
<210> 243
<211> 441
<212> DNA
<213> OTU3143
<400> 243
cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60
cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaatgacg 120
gtacctgact aagaagcacc ggctaactac gtgccagcag ccgcggtaaa acgtaggtca 180
caagcgttgt ccggaattac tgggtgtaaa gggagcgcag gcgggagaac aagttggaag 240
tgaaatccat gggctcaacc catgaactgc tttcaaaact gttttccttg agtagtgcag 300
aggtaggcgg aattcccggt gtagcggtgg aatgcgtaga tatcgggagg aacaccagtg 360
gcgaaggcgg cttactggac ggtaactgac gttgaggctc gaaagcgtgg ggagcaaaca 420
ggattagata ccctagtagt c 441
<210> 244
<211> 460
<212> DNA
<213> OTU3155
<400> 244
cctacgggtg gctgcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60
agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagttatc 120
cacgtgtgga tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggacagtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300
gctgtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc ttactggatt gtaactgacg ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac cccagtagtc 460
<210> 245
<211> 440
<212> DNA
<213> OTU3157
<400> 245
cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60
cgcgtgagtg atgacggcct tcgggttgta aagctctgtc ttcagggacg ataatgacgg 120
tacttgagga ggaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180
tagcgttatc cggaattact gggcgtaaag ggtgcgtaga cggtgtggca agtctgatgt 240
gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300
ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360
cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420
gattagatac ccctgtagtc 440
<210> 246
<211> 460
<212> DNA
<213> OTU3166
<400> 246
cctacgggtg gcagcagtga ggaatattgg tcaatggccg agaggctgaa ccagccaagt 60
cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgtgg 120
gacgtgtcct attttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180
cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240
tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300
gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360
atcacgaaga actccgattg cgaaggcagc tcactagact gtcactgaca ctgatgctcg 420
aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460

Claims (10)

1.一种用于2型糖尿病鉴别的肠道微生物基因标记物组合，其特征在于，包括如下表中所示的246种OTU的组合：
Figure FDA0002748732970000011
Figure FDA0002748732970000021
Figure FDA0002748732970000031
Figure FDA0002748732970000041
2.根据权利要求1所述的用于2型糖尿病鉴别的肠道微生物基因标记物组合，其特征在于，各所述OTU标记物包括如下表中所示序号的核苷酸序列结构：
Figure FDA0002748732970000042
Figure FDA0002748732970000051
3.权利要求1或2所述微生物基因标记物组合用于制备糖尿病患者与健康人鉴别诊断试剂，或者，糖尿病患者的筛查或是辅助诊断，或者，糖尿病微生物靶向治疗药物的用途。
4.根据权利要求3所述的用途，其特征在于，所述糖尿病包括2型糖尿病。
5.一种用于糖尿病鉴别及诊断的试剂盒，其特征在于，包括如权利要求1或2所述的肠道微生物基因标记物组合的引物。
6.根据权利要求5所述的试剂盒，其特征在于，所述引物包括341F_805R，其插入长度为465bq，测序长度为2*300bp，具体结构为：
上游引物：341F 5’-CCTACGGGNGGCWGCAG-3’；
下游引物：805R5’-GACTACHVGGGTATCTAATCC-3’。
7.一种构建预测及辅助诊断糖尿病的模型的方法，其特征在于，包括如下步骤：
(1)收集符合要求的糖尿病人和健康人的大便完成16S rRNA基因测序；
(2)将获得的基因序列，依据97％的相似性完成OTU聚类以及丰度测定；进一步采用RDPclassifier贝叶斯算法对97％相似水平的OTU代表序列进行分类学分析，并分别在各个分类水平统计各样品的群落组成；
(3)基于步骤(2)的结果，通过统计学分析，将有统计学意义的且能够准确区分糖尿病肾病和正常人的OTU筛选出来；
(4)利用步骤(3)筛选出的OTU结果，分别计算在糖尿病人和正常人中富集，且有统计学意义的OTU，并将两类OTU分别相加求和；利用两组OTU总差值和，构建ROC曲线，从而确定此OTU组合标记物对于糖尿病和正常人的区别能力。
8.根据权利要求7所述的预测及辅助诊断糖尿病的模型的构建方法，其特征在于，还包括对待诊断患者的粪便按照权利要求1或2所述微生物基因标记物进行基因检测的步骤，以及，对诊断结果进行验证的步骤。
9.根据权利要求7或8所述的预测及辅助诊断糖尿病的模型的构建方法，其特征在于，所述246种OTU组成中，共有56种在糖尿病患者中增多，190种在糖尿病患者中减少。
10.一种用于微生物靶向治疗糖尿病的组合物，其特征在于，包括如权利要求1或2所述的微生物基因标记物组合，即通过增加或减少相应的所述标记物的相对含量而治疗或者控制糖尿病，对糖尿病进行早期干预，从而控制糖尿病的病情，预防并发症的出现。
CN202011185119.2A 2020-10-28 2020-10-28 一种用于2型糖尿病鉴别的肠道微生物基因标记物组合及其应用 Pending CN115247207A (zh)

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