CN115227718A - Application of nano-silver in inhibiting streptococcus suis - Google Patents
Application of nano-silver in inhibiting streptococcus suis Download PDFInfo
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- CN115227718A CN115227718A CN202210934569.XA CN202210934569A CN115227718A CN 115227718 A CN115227718 A CN 115227718A CN 202210934569 A CN202210934569 A CN 202210934569A CN 115227718 A CN115227718 A CN 115227718A
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- streptococcus suis
- silver
- inhibiting
- nano silver
- nano
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/38—Silver; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
Abstract
The invention belongs to the technical field of nano silver application, and discloses application of nano silver in inhibition of Streptococcus suis, wherein the nano silver is used for inhibiting clinically common Streptococcus suis type 2 and 9 (Streptococcus suis), specifically is used for inhibiting growth of Streptococcus suis, inhibiting formation of a Streptococcus suis biomembrane, causing oxidative stress to Streptococcus suis and reducing Streptococcus suis intracellular protein, and besides, the nano silver can be used for preparing a Streptococcus suis (Streptococcus suis) bactericide and is used for inactivating clinically common Streptococcus suis type 2 and 9, the nano silver is set to be elemental silver, and the particle size range of the elemental silver is set to be 1-100nm. The invention reduces the drug resistance of the streptococcus suis caused by abuse of antibiotics by applying the nano-silver to inhibit the streptococcus suis in a breakthrough manner and preparing a broad-spectrum and high-efficiency streptococcus suis bactericide.
Description
Technical Field
The invention relates to the technical field of nano-silver application, in particular to application of nano-silver in inhibiting streptococcus suis.
Background
The streptococcus suis is a pathogen of streptococcus suis which is a zoonotic infectious disease, and can be divided into 35 serotypes according to different capsular polysaccharide antigens, wherein the serotypes 1, 2, 7 and 9 are common, and the serotype 2 has the strongest toxicity and the widest infection range. The method has the advantages that the method poses great threat to the health of swinery in intensive culture, causes various diseases such as serious brain, arthritis, endocarditis and the like after the infection of people and pigs, and has high mortality rate after the disease attack. Antibiotics are often used for the production of prevention and treatment.
However, with the use of antibiotics and the transmission of bacterial drug resistance for many years, the drug resistance of streptococcus suis has become severe in recent years, which results in poor or even ineffective inhibition effect of common antibiotics on drug-resistant bacteria, so that the search for effective antibiotic substitutes while reducing the abuse of antibiotics is of great significance for the prevention and control of streptococcus suis.
The nano silver is a novel antibacterial material emerging in recent years, and is widely concerned due to the broad-spectrum and high-efficiency antibacterial action, but the application of the nano silver in inhibiting the streptococcus suis is not reported.
Therefore, the application of the nano silver in inhibiting the streptococcus suis is needed to solve the problems.
Disclosure of Invention
The invention aims to provide application of nano silver in inhibiting Streptococcus suis, which solves the defects in the technology by breakthrough application of nano silver in inhibiting Streptococcus suis and preparation of a Streptococcus suis bactericide, wherein broad-spectrum and high-efficiency antibacterial effects of nano silver can effectively inactivate Streptococcus suis type 2 and Streptococcus suis type 9 (Streptococcus suis).
In order to achieve the above purpose, the invention provides the following technical scheme:
application of nano silver in inhibiting streptococcus suis is provided.
As a preferable scheme of the invention, the nano-silver is used for inhibiting clinically common Streptococcus suis (Streptococcus suis) 2 and 9 in Streptococcus suis.
As a preferable scheme of the invention, the nano silver is used for inhibiting the growth of streptococcus suis.
As a preferable scheme of the invention, the nano silver is used for inhibiting the formation of the streptococcus suis biofilm.
As a preferable scheme of the invention, the nano silver causes the oxidative stress of the streptococcus suis.
As a preferable scheme of the invention, the nano silver is used for reducing intracellular protein of streptococcus suis.
Application of nano silver in preparation of streptococcus suis bactericide.
As a preferred embodiment of the invention, the Streptococcus suis bactericide is used for inhibiting and inactivating clinically common Streptococcus suis type 2 and 9 (Streptococcus suis).
As a preferable scheme of the present invention, the nano silver is elemental silver, and the particle size range of the elemental silver is set to 1-100nm.
In the technical scheme, the invention provides the following technical effects and advantages:
by applying the nano silver to inhibition of the Streptococcus suis and preparing the Streptococcus suis bactericide in a breakthrough manner, a new application of the nano silver is discovered, and unexpected technical effects are generated, namely the nano silver has broad-spectrum and high-efficiency antibacterial action and can effectively inactivate the Streptococcus suis types 2 and 9 (Streptococcus suis), reduce the drug resistance of the Streptococcus suis caused by abuse of antibiotics and bacterial drug resistance and safely and effectively inhibit the spread of the Streptococcus suis.
Detailed Description
The present invention will be described in further detail below in order to enable those skilled in the art to better understand the technical solution of the present invention.
The invention provides the following scheme:
the application of the nano-silver in inhibiting the streptococcus suis is found through research, and the nano-silver can effectively inhibit the growth of the streptococcus suis.
Furthermore, in the technical scheme, the nano-silver is used for inhibiting the clinically common Streptococcus suis type 2 and 9 (Streptococcus suis) in the Streptococcus suis, and the inhibiting effect on the Streptococcus suis type 2 and 9 (Streptococcus suis) in the Streptococcus suis is most obvious.
Further, in the technical scheme, the nano silver is used for inhibiting the growth of streptococcus suis.
Furthermore, in the technical scheme, the nano silver is used for inhibiting the formation of the streptococcus suis biofilm.
Furthermore, in the technical scheme, the nano silver causes the oxidative stress of the streptococcus suis.
Furthermore, in the technical scheme, the nano silver is used for reducing the intracellular protein of the streptococcus suis.
Application of nano silver in preparation of streptococcus suis bactericide.
Further, in the technical scheme, the Streptococcus suis bactericide is used for inhibiting and inactivating the clinically common Streptococcus suis 2 and 9 (Streptococcus suis).
Furthermore, in the above technical scheme, the nano silver is elemental silver, the particle size range of the elemental silver is 1-100nm, and the elemental silver with the particle size of 1-100nm can be prepared by physical, chemical, biological and other methods, and researches show that the nano silver has broad-spectrum antibacterial effect.
For the application of nano silver in inhibiting the growth of streptococcus suis, the application realizes research verification by appropriately improving process parameters through a plurality of following examples:
example 1: research on inhibition of growth of streptococcus suis by nano-silver with different concentrations
Selecting a single colony to inoculate in TSB, carrying out shake culture at a constant temperature of 37 ℃ for 8h, calculating the concentration of bacteria by adopting a plate counting method, temporarily storing bacterial liquid in a refrigerator at 4 ℃, inoculating streptococcus suis in a 96-well plate containing the TSB, adding AgNPs solutions with different concentrations to enable the final concentration of the bacteria to be 1.0 multiplied by 106CFU/mL, carrying out double-fold dilution on nano-silver from 100 mu g/mL to 0.098 mu g/mL in an experimental group, not adding the nano-silver in a control group, placing the plate in a constant temperature box at 37 ℃ for 24h, then measuring the absorbance of each hole at a wavelength of 600nm, and calculating the inhibition rate according to the formula as follows:
example 2: research on influence of nano silver with different concentrations on biofilm formation of streptococcus suis
And respectively adding nano silver solution into each bacterial solution to be detected on a 96-well plate to ensure that the final concentration of nano silver is 50, 25, 12.5, 6 and 25 mu g/mL, respectively, setting a control group as the bacterial solution without the nano silver, additionally setting a blank culture medium with the nano silver as a blank control, and performing crystal violet dyeing after standing culture at 37 ℃ for 24 hours. Discarding the culture medium, adding 300 mu L PBS to each hole for washing for 3 times, inverting and draining, adding 150 mu L methanol for fixing for 20min, discarding the solution, inverting and drying, then adding 150 mu L crystal violet solution for dyeing for 15min, sucking out the liquid, washing until no stain exists, adding 150 mu L acetic acid (33%) after drying, eluting for more than 30min, measuring the OD570nm value by using an ultraviolet spectrophotometer, and calculating the inhibition rate according to a formula.
Example 3: research on oxidative stress of streptococcus suis caused by nano-silver
Production of ros was detected by staining bacterial cells with 2'7' -dichlorofluorescein diacetate (H2 DCF-DA). H2DCF-DA was diluted to 1mM with PBS. Adding a nano silver solution in the logarithmic phase of the growth of bacteria, taking a bacterial solution added with PBS in the same amount as a control, washing the solution with PBS after acting for 12 hours, suspending the solution in PBS, adding 1mM H2DCF-DA, incubating the solution for 30min in a dark place, collecting the bacteria, washing and suspending the solution, dripping the bacterial suspension on a glass slide, and naturally drying the solution at room temperature. Observation was performed using a fluorescence microscope.
EXAMPLE 4 investigation of the Effect of nanosilver on intracellular proteins of Streptococcus suis
Adding the streptococcus suis which is incubated to the logarithmic growth stage into TSB according to 1% (V/V), culturing for 12h at the constant temperature of 37 ℃, adding nano-silver into a sample, adding the same amount of PBS into a control group, after 5h of action, centrifuging the sample at the low temperature of 4 ℃ and 4000rpm for 10min, suspending the obtained thalli in a proper amount of sterile water, and mixing a 5X SDS-PEAGE Loading Buffer and the sample by a ratio of 1:4, mixing the mixture in a boiling water bath for 8min, finally centrifuging the mixture for 5min at 9000rmp, carrying out SDS-PEAGE electrophoresis on the obtained supernatant, dyeing the supernatant for 40min by Coomassie brilliant blue, decolorizing the decolorized solution for multiple times until protein bands are clearly visible, and carrying out imaging shooting on a daylight lamp panel.
The research of the above examples shows that the nano silver can inhibit the growth of the streptococcus suis, can inhibit the formation of a biofilm of the streptococcus suis, can cause the oxidative stress of the streptococcus suis and can reduce the intracellular protein of the streptococcus suis.
The inhibitor can effectively inhibit the growth of Streptococcus suis type 2 and 9 (Streptococcus suis), and can be used for preparing a Streptococcus suis growth inhibitor.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the invention. Accordingly, the foregoing description is illustrative in nature and is not to be construed as limiting the scope of the invention as claimed.
Claims (9)
1. Application of nano silver in inhibiting streptococcus suis is provided.
2. The use of nanosilver according to claim 1 for inhibiting streptococcus suis, characterized in that: the nano silver is used for inhibiting clinically common Streptococcus suis 2 and 9 (Streptococcus suis) in Streptococcus suis.
3. The use of nanosilver according to claim 2 for inhibiting streptococcus suis, wherein: the nano silver is used for inhibiting the growth of streptococcus suis.
4. The use of nanosilver according to claim 2 for inhibiting streptococcus suis, wherein: the nano silver is used for inhibiting the formation of a streptococcus suis biofilm.
5. The use of nanosilver according to claim 2 for inhibiting streptococcus suis, wherein: the nano silver causes the oxidative stress of streptococcus suis.
6. The use of nanosilver according to claim 2 for inhibiting streptococcus suis, wherein: the nano silver is used for reducing intracellular protein of streptococcus suis.
7. Use of nano silver as claimed in any one of claims 1 to 6 in the preparation of a streptococcus suis fungicide.
8. The application of the nano silver in the preparation of the streptococcus suis bactericide according to claim 7 is characterized in that: the Streptococcus suis bactericide is used for inhibiting and inactivating clinically common Streptococcus suis 2 and 9 (Streptococcus suis).
9. The use of nanosilver according to claim 7 for inhibiting streptococcus suis, wherein: the nano silver is set to be simple substance silver, and the particle size range of the simple substance silver is set to be 1-100nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210934569.XA CN115227718A (en) | 2022-08-04 | 2022-08-04 | Application of nano-silver in inhibiting streptococcus suis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210934569.XA CN115227718A (en) | 2022-08-04 | 2022-08-04 | Application of nano-silver in inhibiting streptococcus suis |
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| CN115227718A true CN115227718A (en) | 2022-10-25 |
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| CN202210934569.XA Pending CN115227718A (en) | 2022-08-04 | 2022-08-04 | Application of nano-silver in inhibiting streptococcus suis |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020051823A1 (en) * | 2000-09-13 | 2002-05-02 | Jixiong Yan | Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same |
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- 2022-08-04 CN CN202210934569.XA patent/CN115227718A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020051823A1 (en) * | 2000-09-13 | 2002-05-02 | Jixiong Yan | Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same |
Non-Patent Citations (1)
| Title |
|---|
| MAIS EMAD.AHMED等: "Effects of Silver Nanoparticles on Biofilms of Streptococcus Spps", 《INDIAN JOURNAL OF PUBLIC HEALTH RESEARCH & DEVELOPMENT》, vol. 9, no. 12, pages 1216 - 1221 * |
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