CN115227438A - Rat caudal vertebra dynamic loading device - Google Patents

Rat caudal vertebra dynamic loading device Download PDF

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Publication number
CN115227438A
CN115227438A CN202210772452.6A CN202210772452A CN115227438A CN 115227438 A CN115227438 A CN 115227438A CN 202210772452 A CN202210772452 A CN 202210772452A CN 115227438 A CN115227438 A CN 115227438A
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China
Prior art keywords
guide rail
retainer
dynamic loading
base
pull rod
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CN202210772452.6A
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Chinese (zh)
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霍波
高颜
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Beijing Institute of Technology BIT
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Beijing Institute of Technology BIT
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Priority to CN202210772452.6A priority Critical patent/CN115227438A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D1/00—Surgical instruments for veterinary use
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/45—For evaluating or diagnosing the musculoskeletal system or teeth
    • A61B5/4504—Bones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D3/00—Appliances for supporting or fettering animals for operative purposes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2503/00—Evaluating a particular growth phase or type of persons or animals
    • A61B2503/40—Animals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2503/00—Evaluating a particular growth phase or type of persons or animals
    • A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D3/00—Appliances for supporting or fettering animals for operative purposes
    • A61D2003/003—Appliances for supporting or fettering animals for operative purposes with head or neck restraining means

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Surgery (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Rheumatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Animal Husbandry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dentistry (AREA)
  • Orthopedics, Nursing, And Contraception (AREA)

Abstract

The invention provides a rat caudal vertebra dynamic loading device which is simple in structure and capable of dynamically adjusting the loading direction and the loading frequency. This dynamic loading device includes: the device comprises a control unit, a base and a dynamic loading unit arranged on the base; the dynamic loading unit comprises: the device comprises a driving unit, a guide rail, a movable pull rod and a retainer; the guide rail mounting seat and the retainer are respectively fixed at two ends of the same straight line of the base; the movable pull rod is in sliding fit with a guide rail arranged on the guide rail mounting seat; the driving unit drives the movable pull rod to slide along the guide rail in a reciprocating manner under the control of the control unit; the retainer is used for fixing a test mouse body, and a through hole for allowing a mouse tail to penetrate out is formed in the end part, facing one end of the guide rail mounting seat, of the retainer; a tail fixing end is arranged on the base between the guide rail mounting seat and the retainer and is used for fixing a tail vertebra of a tail at a set position; the end part of the movable pull rod facing one end of the retainer is also provided with a tail fixing end.

Description

Dynamic loading device for tail vertebrae
Technical Field
The invention relates to a loading device, in particular to a bone tissue mechanics loading device, and belongs to the technical field of bone tissue mechanics loading.
Background
Bones are important organs for maintaining human life, constitute a human body support, are important components of a human body movement system, and have the functions of supporting, protecting and moving. Bone tissue is sensitive to mechanical stimuli and can adapt to a constantly changing mechanical environment by constantly adjusting the external morphology and the internal tissue structure of the bone tissue. Mechanical stimulation to change bone morphology is critical to maintaining bone performance, and when people engage in various activities, such as climbing stairs, jogging, or standing in place, they exert loads on the bone and cause various degrees of deformation of the bone matrix.
As for the regulation mechanism of bone remodeling caused by force, different researches have been made from molecules, cells to tissues at home and abroad. In vivo experiments, mice are usually selected for relevant studies. The existing bone tissue mechanics loading experimental device at present comprises a dynamic loading device which performs static stretching and compression on a tail vertebra of a rat or performs in-vitro tissue loading under a body state, and the existing bone tissue mechanics loading device mainly has the following defects:
(1) The static loading device cannot adjust the loading frequency;
(2) The traditional loading device has a plurality of redundant structures, so that the mechanical loss is large, and the actual force acting on a sample cannot be accurately measured.
(3) The evolution process of the trabecular bone microstructure under the action of long-term mechanical load and the related cell distribution rule cannot be known by the in vitro loaded sample.
There is no good experimental set-up for bone overload and precisely controlled dynamic loading. But quantitative analysis on the external load change of the bone, the bone mass and the bone structure has very important significance for disclosing and exploring the regulation mechanism of bone reconstruction caused by force.
Disclosure of Invention
In view of this, the invention provides a dynamic loading device for a caudal vertebra, which has a simple structure and can dynamically adjust the loading direction and the loading frequency.
The technical scheme of the invention is as follows: a caudal vertebra dynamic loading device, comprising: the device comprises a control unit, a base and a dynamic loading unit arranged on the base;
the dynamic loading unit comprises: the device comprises a driving unit, a guide rail, a movable pull rod and a retainer;
the guide rail mounting seat and the retainer are respectively fixed at two ends of the same straight line of the base; the movable pull rod is in sliding fit with a guide rail arranged on the guide rail mounting seat;
the driving unit drives the movable pull rod to slide along the guide rail in a reciprocating manner under the control of the control unit;
the retainer is used for fixing a test mouse body, and a through hole for allowing a mouse tail to penetrate out is formed in the end part, facing one end of the guide rail mounting seat, of the retainer;
a base between the guide rail mounting seat and the retainer is provided with a tail fixing end for fixing a tail vertebra at a set position;
and a tail fixing end is also arranged at the end part of one end of the movable pull rod, which faces to the retainer.
On the basis of the above scheme, further, the driving unit includes: a motor, a cam and a connecting rod;
the motor is fixed on the guide rail mounting seat through the motor seat;
the cam is fixedly arranged on an output shaft of the motor;
one end of the connecting rod is supported outside the cam through a center hole, and the other end of the connecting rod is connected with the movable pull rod through a connecting seat; when the motor drives the cam to rotate, the cam drives the movable pull rod to slide along the guide rail in a reciprocating mode through the connecting rod.
On the basis of the scheme, a mechanical sensor is further fixedly connected between the connecting seat and the movable pull rod; the mechanical sensor is electrically connected with the control unit.
On the basis of above-mentioned scheme, it is further, the tail stiff end is in the position of base is adjustable, makes the distance between removal pull rod and the tail stiff end is adjustable.
On the basis of the above scheme, further, a display is arranged in the control unit, and dynamic mechanical parameters obtained by the mechanical sensor are displayed in real time.
On the basis of the above scheme, further, more than two dynamic loading units are arranged on the base in parallel, and the control unit respectively and independently controls each driving unit.
On the basis of the scheme, the surface of the tail fixing end is provided with a groove, and the tail fixing end is matched with a pressing sheet A arranged above the groove to fix the tail vertebrae of the tail.
On the basis of the scheme, furthermore, the end part of the movable pull rod, which faces one end of the retainer, extends upwards to form a protrusion, the surface of the protrusion is provided with a groove, and the pressing sheet B arranged above the protrusion is matched to fix the caudal vertebra of the tail.
Has the advantages that:
(1) The device can automatically perform dynamic cyclic loading on the caudal vertebra at a set position under a set amplitude, and the whole device is controlled by the control unit freely to adjust and control required dynamic loading conditions, so that the operation is simple and safe.
(2) The dynamic loading unit adopts a cam to control the loading stroke, so that high-frequency cyclic dynamic loading can be realized.
(3) And the dynamic loading unit is provided with a force sensor which can record and store the actual acting force acting on the coccyx in the loading process.
(4) The position of the rat tail fixed end on the base is adjustable, so that the distance between the movable pull rod and the rat tail fixed end can be flexibly adjusted; therefore, the boundary condition of the displacement loading of the caudal vertebra can be changed conveniently, and the device can realize the static loading of the caudal vertebra.
(5) A plurality of dynamic loading units are arranged on the base, so that dynamic loading of different directions and frequencies can be simultaneously performed on a plurality of experimental mice.
Drawings
FIG. 1 is a front view of the dynamic loading unit of the present invention (excluding the base and control unit);
FIG. 2 is an exploded view of the dynamic loading unit of the present invention (excluding the base and control unit);
fig. 3 is a top view of a loading device having four dynamic loading modules.
Wherein: 1-a motor; 2-a connecting rod; 3-a cam; 4-a motor base; 5-a guide rail mounting seat; 6-a force sensor; 7-a guide rail; 8-moving the pull rod; 9-rat tail fixed end; 10-tabletting A; 11-tabletting B; 12-a retainer; 13-a base; 14-control unit
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Example 1:
the embodiment provides a dynamic loading device for a caudal vertebra, which is an in-vivo caudal vertebra loading system capable of freely regulating and controlling the loading direction and frequency, and can be used for researching the microstructure change rule of an endosseous trabecula in a body state and the relation between related cytological distributions, so as to explore a molecular biological mechanism of bone tissue reconstruction caused by force.
As shown in fig. 1 to 3, the loading device includes: the device comprises a control unit, a base 13 and a dynamic loading unit arranged on the base 13; the dynamic loading unit comprises: the device comprises a motor 1, a cam 3, a connecting rod 2, a guide rail mounting seat 5, a guide rail 7, a movable pull rod 8 and a retainer 12;
the guide rail mounting seat 5 and the retainer 12 are respectively fixed at two ends of the same straight line of the base 13; the motor 1 is fixed on the guide rail mounting base 5 through a motor base 4 (specifically, the end part of an output shaft of the motor 1 is supported on the motor base 4 through a bearing 3); the cam 3 is fixedly arranged on an output shaft of the motor 1 through a fastener (wherein the circle center of a base circle of the cam 3 is positioned on the axis of the output shaft of the motor), one end of the connecting rod 2 is supported outside the cam 3 through a center hole (the circle center of an eccentric circle of the cam 3 is positioned on the axis of a center line hole of the connecting rod 2), the other end of the connecting rod is connected with the movable pull rod 8 through a connecting seat, and a mechanical sensor 6 is fixedly connected between the connecting seat and the movable pull rod 8; the movable pull rod 8 and the bottom surface of the connecting seat are in sliding fit with a guide rail 7 arranged on the guide rail mounting seat 5. The motor 1 provides power for the cam 3, and sinusoidal motion is provided through the cam 3, and when the motor 1 drives the cam 3 to rotate, the cam 3 drives the movable pull rod 8 to slide along the guide rail 7 in a reciprocating mode through the connecting rod 2. The amplitude is determined according to the eccentricity of the cam 3 (i.e., the distance between the center of the base circle of the cam 3 and the center of the eccentric circle) to ensure that the stretching distance is a set value (i.e., the reciprocating distance of the movable pull rod 8 is a set value). Therefore, the dynamic cyclic loading with the same amplitude can be carried out on the set caudal vertebra position through the dynamic loading unit; the adjustment of the dynamic cyclic loading amplitude can be achieved by varying the eccentricity of the cam 3.
The mechanical sensor 6 is electrically connected with the control unit and is used for detecting the stress condition of the movable pull rod 8 in the dynamic loading process in real time and sending the detection result to the control unit, so that the stress of the caudal vertebra in the loading process is recorded and transmitted in real time; in addition, the control unit controls the start, stop, rotating speed and rotating direction of the motor 1 to realize the control of the dynamic loading switch, frequency and direction of the whole device.
The retainer 12 is arranged on the base 13 and is used for fixing a living rat; the end part of the retainer 12 is provided with a through hole for the mouse tail to penetrate out; when in use, the head and the body of a living rat are placed in the retainer 12, and the rat tail penetrates through the through hole at the end part of the retainer 12.
A tail fixing end 9 is arranged on the base 13 and between the guide rail 7 and the retainer 12 and is used for fixing tail vertebrae at a set position; in this example, the 7 th caudal vertebra is fixed to the caudal fixation end 9; the surface of the tail fixing end 9 is provided with a groove, and the tail vertebra of the tail is fixed by matching with a pressing sheet A11 arranged above the groove; specifically, the method comprises the following steps: the lower end face of the pressing sheet A11 is also provided with a groove, a through hole for allowing the rat tail to pass through is formed after the pressing sheet A11 is butted with the groove on the surface of the rat tail fixing end 9, and then the pressing sheet A11 is fixed on the surface of the rat tail fixing end 9 through a fastening piece, so that the clamping and fixing of the rat tail vertebra at the position are realized.
The end of the movable pull rod 8 facing one end of the retainer 12 is also provided with a rat tail fixing end, namely, the end of the movable pull rod 8 facing one end of the retainer 12 extends upwards to form a bulge, the surface of the bulge is provided with a groove, and a pressing sheet B10 arranged above the bulge is matched to fix the rat tail vertebra (the same way as the matching way of the pressing sheet A11 and the rat tail fixing end 9 is adopted); in this example, the 9 th caudal vertebra is fixed to a caudal fixation end of a traveling pull rod 8. Thereby, the eighth caudal vertebra is positioned between the moving rod 8 and the rat tail fixing end 9.
In this example, the seventh and ninth caudal vertebrae of the laboratory mouse are fixed, so that the eighth caudal vertebra in the middle can be dynamically loaded by controlling the movement of the movable pull rod 8, and loads in different directions and different frequencies can be applied according to experimental needs. The dynamic loading unit adopts a cam 3 to control the loading stroke so as to realize high-frequency cyclic dynamic loading.
The dynamic loading device for circularly and dynamically loading the tail vertebrae comprises the following steps:
firstly, the head of the live rat for the test faces towards the retainer 12, the tail of the live rat faces towards the movable pull rod 8 and is placed in the retainer 12, and the tail is exposed. Then, the 7 th caudal vertebra is clamped and fixed through a caudal fixing end 9, and the 9 th caudal vertebra is clamped and fixed through a caudal fixing end on a movable pull rod 8; then, the motor 1 is started through the control unit 14, and the eighth caudal vertebra is subjected to cyclic dynamic loading through the movable pull rod 8; the direction and frequency of dynamic loading are adjusted by the control unit 14, the obtained dynamic mechanical parameters are stored in the control unit 14 by the mechanical sensor 6 and are obtained by a memory card, and subsequent experimental treatment can be performed in a computer.
In order to obtain the mechanical parameters visually, a display may be provided in the control unit 14 to display the dynamic mechanical parameters obtained by the mechanical sensor 6 in real time.
In addition, position at base 13 is adjustable for rat tail stiff end 9 (can set up the mounting hole of multiunit rat tail stiff end 9 along the moving direction of removal pull rod 8 on base 13, during the experiment, selects the mounted position of rat tail stiff end 9 according to experimental demand), makes the distance that removes between pull rod 8 and the rat tail stiff end 9 can be adjusted in a flexible way. Therefore, the boundary condition of the displacement loading of the caudal vertebra can be conveniently changed, and the device can also realize the static loading of the caudal vertebra, namely, the caudal vertebra between the caudal fixing end 9 and the movable pull rod 8 is also in a stretching or compressing state under the static non-operating state of the dynamic loading unit so as to realize the static compression or stretching. Simultaneously, the position of tail stiff end 9 at base 13 is adjustable also makes the device can adapt to different test mouse.
Example 2:
on the basis of the above embodiment 1, the number of experimental groups can be increased to efficiently and synchronously complete multiple groups of experiments with different loading directions and frequencies.
As shown in fig. 3, a plurality of dynamic loading units are arranged on the base 13 in parallel, the motors 1 in the dynamic loading units are respectively connected with the control unit 14, and the control unit respectively controls the start, stop, rotation speed and rotation direction of each motor 1; therefore, the accurate regulation and control of different directions and frequencies of multiple experimental mice can be provided simultaneously.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, it is intended that all such modifications and alterations be included within the scope of this invention as defined in the appended claims.

Claims (8)

1. A caudal vertebra dynamic loading device, comprising: the device comprises a control unit, a base (13) and a dynamic loading unit arranged on the base (13);
the dynamic loading unit comprises: a driving unit, a guide rail (7), a movable pull rod (8) and a retainer (12);
the guide rail mounting seat (5) and the retainer (12) are respectively fixed at two ends of the same straight line of the base (13); the movable pull rod (8) is in sliding fit with a guide rail (7) arranged on the guide rail mounting seat (5);
the driving unit drives the movable pull rod (8) to slide along the guide rail (7) in a reciprocating manner under the control of the control unit;
the retainer (12) is used for fixing a test mouse body, and a through hole for allowing a mouse tail to penetrate out is formed in the end part, facing one end of the guide rail mounting seat (5), of the retainer (12);
a rat tail fixing end (9) is arranged on a base (13) between the guide rail mounting seat (5) and the retainer (12) and used for fixing rat tail vertebrae at a set position;
the end part of the movable pull rod (8) facing one end of the retainer (12) is also provided with a rat tail fixing end.
2. The caudal vertebra dynamic loading device of claim 1, wherein said drive unit comprises: the device comprises a motor (1), a cam (3) and a connecting rod (2);
the motor (1) is fixed on the guide rail mounting base (5) through a motor base (4);
the cam (3) is fixedly arranged on an output shaft of the motor (1);
one end of the connecting rod (2) is supported outside the cam (3) through a central hole, and the other end of the connecting rod is connected with the movable pull rod (8) through a connecting seat; when the motor (1) drives the cam (3) to rotate, the cam (3) drives the movable pull rod (8) to slide back and forth along the guide rail (7) through the connecting rod (2).
3. A caudal vertebra dynamic loading device according to claim 2, wherein a mechanical sensor (6) is fixed between said connecting seat and said moving rod (8); the mechanical sensor (6) is electrically connected with the control unit.
4. A caudal vertebra dynamic loading device according to claim 1, wherein said fixed caudal end (9) is adjustable in position on said base (13) such that the distance between said movable rod (8) and said fixed caudal end (9) is adjustable.
5. The caudal vertebra dynamic loading device according to claim 3, wherein a display is provided in the control unit (14) for displaying the dynamic mechanical parameters acquired by the mechanical sensor (6) in real time.
6. A caudal vertebra dynamic loading device according to any of claims 1-5, wherein more than two dynamic loading units are arranged side by side on said base (13), and said control unit controls each driving unit independently.
7. The caudal vertebra dynamic loading device according to any one of claims 1 to 5, wherein the caudal fixation end (9) has a groove on its surface for engaging with a pressing plate A (11) disposed thereon to fix the caudal vertebra.
8. A dynamic rat caudal vertebra loading device according to any one of claims 1 to 5, wherein said movable rod (8) has a projection extending upwardly towards the end of the retainer (12), the projection having a recess on its surface for engaging a pressing plate B (10) provided thereon for fixation of the rat caudal vertebra.
CN202210772452.6A 2022-06-30 2022-06-30 Rat caudal vertebra dynamic loading device Pending CN115227438A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210772452.6A CN115227438A (en) 2022-06-30 2022-06-30 Rat caudal vertebra dynamic loading device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210772452.6A CN115227438A (en) 2022-06-30 2022-06-30 Rat caudal vertebra dynamic loading device

Publications (1)

Publication Number Publication Date
CN115227438A true CN115227438A (en) 2022-10-25

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Application Number Title Priority Date Filing Date
CN202210772452.6A Pending CN115227438A (en) 2022-06-30 2022-06-30 Rat caudal vertebra dynamic loading device

Country Status (1)

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CN (1) CN115227438A (en)

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