CN115212932A - Microfluidic chip assembly - Google Patents
Microfluidic chip assembly Download PDFInfo
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- CN115212932A CN115212932A CN202110418205.1A CN202110418205A CN115212932A CN 115212932 A CN115212932 A CN 115212932A CN 202110418205 A CN202110418205 A CN 202110418205A CN 115212932 A CN115212932 A CN 115212932A
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- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 117
- 238000007789 sealing Methods 0.000 claims abstract description 107
- 238000003860 storage Methods 0.000 claims abstract description 42
- 238000005192 partition Methods 0.000 claims description 12
- 230000007704 transition Effects 0.000 claims description 11
- 238000011144 upstream manufacturing Methods 0.000 claims description 9
- 238000004166 bioassay Methods 0.000 abstract description 2
- 238000007689 inspection Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 description 16
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 238000005842 biochemical reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000006870 function Effects 0.000 description 3
- 108010066057 cabin-1 Proteins 0.000 description 2
- 239000002274 desiccant Substances 0.000 description 2
- 238000002032 lab-on-a-chip Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000007731 hot pressing Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
The invention discloses a microfluidic chip assembly, relates to the technical field of biological assay or inspection, and is used for optimizing the performance of the microfluidic chip assembly. The microfluidic chip assembly comprises a reagent bin, a reagent bin bracket and a chip body. The reagent cabin comprises a cabin body, a first sealing element and a second sealing element; the storehouse body has the storage chamber, and the one end of the storehouse body is provided with first sealing member, and the other end of the storehouse body is provided with the second sealing member more than two-layer. The reagent cartridge support has a first puncture needle. The chip body is provided with a second puncture needle; the chip body can be dismantled with reagent storehouse support and be connected, and chip body and reagent storehouse support form and place the region to hold the reagent storehouse. Wherein the first lancet corresponds to the first seal and the second lancet corresponds to the second seal. The microfluidic chip assembly provided by the technical scheme has good sealing performance, reduces the possibility of substance leakage in the storage cavity of the reagent bin, and improves the use reliability of the microfluidic chip assembly.
Description
Technical Field
The invention relates to the technical field of biological assay or inspection, in particular to a microfluidic chip assembly.
Background
Nucleic acid detection is one of the current gold standards for infectious disease diagnosis. The traditional nucleic acid detection method has complicated steps and has high requirements on detection fields and qualification of operators. The microfluidic chip is also called as a lab-on-a-chip (lab-on-a-chip), and can realize high-integration and integrated point-of-care test (POCT) based on the microfluidic chip technology, thereby greatly shortening the time required by detection and realizing the detection at any time.
The micro-fluidic chip is provided with a plurality of small-sized flow channels and cavities, the flow channels are arranged in a certain rule, in the testing process, different biological reagents are released according to a certain sequence and flow to the designated cavity through the different flow channels to complete various biochemical reactions, and finally, the rapid and accurate detection of nucleic acid is realized.
Disclosure of Invention
An embodiment of the present invention provides a microfluidic chip assembly, including:
the reagent bin comprises a bin body, a first sealing element and a second sealing element; the bin body is provided with a storage cavity, one end of the bin body is provided with the first sealing element, and the other end of the bin body is provided with more than two layers of the second sealing elements;
a reagent cartridge holder having a first piercing needle; and
a chip body having a second lancet; the chip body is detachably connected with the reagent bin support, and the chip body and the reagent bin support form a placing area for accommodating the reagent bin;
wherein the first lancet corresponds to the first seal and the second lancet corresponds to the second seal.
In some embodiments, the storage chamber is divided into a storage section, a throat section, and a sealing section along an axial direction of the reagent cartridge; the narrow section has an inner diameter dimension that is less than the respective inner diameter dimensions of the storage section and the sealing section.
In some embodiments, the second sealing members are located in the sealing section, and each of the second sealing members is disposed in a dispersed manner along an axial direction of the reagent cartridge.
In some embodiments, the seal section comprises a transition section and a mounting section; the inner diameter of the transition section is smaller than that of the mounting section; the transition section is connected to the throat section.
In some embodiments, the microfluidic chip assembly further comprises:
a seal ring disposed at the mounting section of the sealing section, the seal ring configured to seal against an outer wall of a second puncture needle that punctures the second sealing element.
In some embodiments, at least one of the second seals is located upstream of the seal ring, and the remaining second seals are located downstream of the seal ring.
In some embodiments, the microfluidic chip assembly further comprises:
a partition disposed inside the memory segment to divide the memory segment into a plurality of regions; the partition is provided with a through hole therethrough to communicate the respective regions.
In some embodiments, the inner wall of the storage section is provided with a mounting portion that is convex or concave toward the inside of the storage section, and the partition is mounted to the mounting portion.
In some embodiments, the reagent cartridge holder comprises:
the plate body is detachably connected with the chip body; and
the top cover is rotatably arranged at one end of the plate body and is detachably connected with the chip body;
wherein the top cover is positioned outside of the first seal of the reagent cartridge and the second lancet is positioned outside of the second seal; the plate body, the top cover and the chip body jointly wrap the reagent bin.
In some embodiments, the top cap comprises a cap body, a first snap, and a pusher; one side of the cover body is rotatably connected with the plate body; the first buckle is arranged on one side of the top cover far away from the plate body and is constructed to buckle the chip body; the propeller is configured to be annular and is mounted to the top cover; wherein the first lancet is mounted inside the pusher; and/or the presence of a gas in the atmosphere,
the plate body is provided with a second buckle which is constructed to buckle the chip body.
In some embodiments, the chip body comprises:
the connecting part is provided with a third buckle and an inner concave part, the third buckle is connected with the second buckle in a clamping way, and the first buckle is inserted into the inner concave part; and
and the microfluidic part is provided with a microfluidic channel, and the second puncture needle is communicated with the microfluidic channel.
In some embodiments, at least one of the first and second lancet is arranged in a row.
In some embodiments, at least one of the first seal and the second seal comprises a sealing membrane.
According to the microfluidic chip assembly provided by the technical scheme, the other end of the reagent bin is provided with more than two layers of second sealing parts, the multiple layers of second sealing parts are matched with the second puncture needle of the chip body, and after the second puncture needle of the chip body punctures the second sealing parts, the second sealing parts are provided with two layers, so that a double-layer sealing effect can be achieved, the possibility of substance leakage in the storage cavity of the reagent bin is reduced, and the use reliability of the microfluidic chip assembly is improved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
fig. 1 is an exploded schematic view of a microfluidic chip assembly according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a reagent chamber structure of a microfluidic chip assembly according to an embodiment of the present invention;
FIG. 3a is a schematic diagram of a reagent chamber structure of a microfluidic chip assembly according to another embodiment of the present invention;
fig. 3b is a schematic diagram of a spacer structure of a microfluidic chip assembly according to another embodiment of the present invention;
FIG. 4 is a schematic diagram of a reagent cartridge holder structure of a microfluidic chip assembly according to an embodiment of the present invention;
fig. 5 is a schematic partial three-dimensional structure diagram of a chip body of a microfluidic chip assembly according to an embodiment of the present invention;
fig. 6 is a schematic front view of a chip body of a microfluidic chip assembly according to an embodiment of the present invention.
Detailed Description
The technical solution provided by the present invention is explained in more detail with reference to fig. 1 to 6.
Referring to fig. 1, an embodiment of the present invention provides a microfluidic chip assembly, including a reagent cartridge 1, a reagent cartridge holder 2, and a chip body 3. Referring to fig. 2, the reagent cartridge 1 comprises a cartridge body 11, a first sealing member 12 and a second sealing member 13. The bin body 11 is provided with a storage cavity 10, one end of the bin body 11 is provided with a first sealing member 12, and the other end of the bin body 11 is provided with more than two layers of second sealing members 13. Referring to fig. 4, the reagent cartridge holder 2 has a first lancet 21. Referring to fig. 1, 5 and 6, the chip body 3 has a second lancet 31; chip body 3 can dismantle with reagent storehouse support 2 and be connected, and chip body 3 and reagent storehouse support 2 form and place the region to hold reagent storehouse 1. Wherein the first lancet 21 corresponds to the first seal 12 and the second lancet 31 corresponds to the second seal 13.
Referring to fig. 1, a reagent cartridge holder 2 is matched with a chip body 3 to support and fix a reagent cartridge 1 and to provide constraint for the movement of the reagent cartridge 1 along the vertical direction shown in fig. 1, so that the reagent cartridge 1 cannot fall off, and the reagent cartridge 1 can be effectively fixed in an unused state and a used state of a microfluidic chip assembly. The unused state refers to a state of the first seal member 12 and the second seal member 13 before being pierced; the use state refers to a state in which the microfluidic chip assembly is used for testing, that is, the first sealing member 12 and the second sealing member 13 are punctured to release the reagent in the storage chamber 10 of the reagent cartridge 1.
The first piercing needle 21 corresponds to the first sealing member 12, which means that after the components of the microfluidic chip assembly are mounted in place, the first piercing needle 21 is located near the first sealing member 12, and taking the direction shown in fig. 1 as an example, the first piercing needle 21 is located above the first sealing member 12; when it is required for subsequent use, the first lancet 21 is caused to pierce the first seal 12 by changing the distance between the first lancet 21 and the first seal 12.
The second piercing needle 31 corresponds to the second sealing member 13, which means that after the components of the microfluidic chip assembly are mounted in place, the second piercing needle 31 is located near the second sealing member 13, and taking the orientation shown in fig. 1 as an example, the second piercing needle 31 is located below the second sealing member 13. When it is needed for subsequent use, the second puncture needle 31 punctures the second sealing member 13 by changing the distance between the second puncture needle 31 and the second sealing member 13.
The storage chamber 10 of the reagent cartridge 1 is used for storing a liquid reagent or a lyophilized reagent. The data of the reagent chamber 1 is determined according to the number of the second puncture needles 31 of the chip body 3, and can be flexibly increased or decreased according to the flow requirements of biochemical reactions. The reagent chambers 1 are arranged in rows, and each reagent chamber 1 is correspondingly provided with a first puncture needle 21 and a second puncture needle 31. All the reagent chambers 1 are fixed together to form a whole reagent chamber.
The first sealing member 12 and the second sealing members 13 of the reagent cabin 1 realize the whole-course sealing during the process of releasing the reagent in the storage chamber 10. The second sealing members 13 are mounted by heat-pressure sealing, and the first sealing member 12 is heat-pressure sealed after the reagent is loaded.
Referring to fig. 2, in some embodiments, along the axial direction of the reagent cartridge 1, i.e. the length direction of the reagent cartridge 1, the storage chamber 10 is divided into a storage section 101, a narrow section 102 and a sealing section 103. The narrow section 102 is located between the storage section 101 and the sealing section 103. The narrow section 102 has an inner diameter dimension that is less than the inner diameter dimension of each of the storage section 101 and the sealing section 103.
The inner diameter of the storage section 101 is configured to be tapered, with the inner diameter of the storage section 101 on the side toward the narrowed section 102 being smaller in size and the inner diameter of the storage section 101 distal from the narrowed section 102 being larger in size to facilitate subsequent release of the reagent.
The inner diameter of the throat section 102 is relatively small in size, and when desired, the inner diameter of the throat section 102 may be set relatively small to allow a small gap between the throat section 102 and the outer wall of the second lancet 31 that has punctured the second seal 13.
Referring to fig. 2, in some embodiments, the second sealing members 13 are located at the sealing section 103, and the second sealing members 13 are distributed along the axial direction of the reagent cartridge 1. The second sealing members 13 are, for example, of a sheet-like structure, and each second sealing member 13 is individually installed to collectively perform a multiple sealing function.
With continued reference to fig. 2, in some embodiments, the seal segment 103 includes a transition segment 103a and a mounting segment 103b. The transition section 103a has an inner diameter dimension that is smaller than the inner diameter dimension of the mounting section 103b. The transition section 103a is located between the throat section 102 and the mounting section 103b. The transition section 103a is connected to the throat section 102. The transition section 103a and the mounting section 103b each have an inner diameter dimension that is greater than the inner diameter dimension of the throat section 102.
With continued reference to fig. 2, in some embodiments, the microfluidic chip assembly further comprises a sealing ring 4, the sealing ring 4 being disposed at the mounting section 103b of the sealing section 103, the sealing ring 4 being configured to seal against an outer wall of the second lancet 31 that has punctured the second sealing member 13. The seal ring 4 is made of rubber, for example.
The microfluidic chip assembly with the sealing ring 4 is particularly suitable for liquid reagents. During transportation or in the case that neither the first sealing member 12 nor the second sealing member 13 at the end of the reagent cartridge 1 is punctured, the liquid reagent can be well sealed by the first sealing member 12 at the top and the multiple layers of second sealing members 13 at the bottom of the reagent cartridge 1.
In the process of using the microfluidic chip assembly, a downward force, i.e. a force towards the direction of the second puncture needle 31, is applied to the reagent chamber bracket 2, the second puncture needle 31 of the chip body 3 is adopted to puncture the second sealing element 13 at the bottom of the reagent chamber 1, and at the moment, the outer wall of the second puncture needle 31 is tightly wrapped by the sealing ring 4 to prevent leakage; then, continuing to apply a downward force to the reagent cartridge support 2, the first piercing needle 21 of the reagent cartridge support 2 pierces the first seal 12 at the top of the reagent cartridge 1, so that the storage chamber 10 of the reagent cartridge 1 is vented to the outside atmosphere to effect release of the reagent. At this time, the liquid in the storage chamber 10 does not leak due to the sealing connection between the seal ring 4 and the second lancet 31 of the chip body 3.
Referring to fig. 2, in some embodiments, at least one of the second seals 13 is located upstream of the seal ring 4, and the remaining second seals 13 are located downstream of the seal ring 4. At least one of the plurality of second seals 13 is located upstream of the seal ring 4, and the remaining second seals 13 are located downstream of the seal ring 4.
After the sealing ring 4 is arranged, due to the sealing effect of the second sealing piece 13 positioned at the upstream of the sealing ring 4, the reagent bin 1 is prevented from being soaked in the reagent in the storage process, so that the service life of the sealing ring 4 is shortened, and the reagent is prevented from reacting with the sealing ring 4 to cause reagent deterioration and invalidation.
Therefore, the technical scheme improves the sealing performance of the microfluidic chip assembly in the unused state and improves the sealing performance of the microfluidic chip assembly in the used state.
Referring to fig. 3a and 3b, in further embodiments, the microfluidic chip assembly further comprises a separator 5, the separator 5 being disposed inside the storage section 101 to divide the storage section 101 into a plurality of regions, each region being configured to place a biological material. The partition 5 is provided with through holes 50 penetrating to communicate the respective regions.
The microfluidic chip assembly may not be provided with a sealing ring 4 if it is suitable for freeze-drying reagents. At this time, the storage section 101 is divided into a plurality of sections by using the partition 5, and each section may be placed with a different lyophilized reagent as shown in fig. 3a and 3b. At the moment, the second sealing element 13 at the bottom of the reagent bin 1 also plays a role in double-layer sealing, so that the sealing performance of the microfluidic chip assembly is improved, and the rapid nucleic acid field detection is favorably realized.
When the reagent lyophilized spheres are loaded, the section downstream of the partition 5 contains the lyophilized spheres and the section upstream of the partition 5 contains a desiccant to extend the shelf life of the lyophilized spheres.
Referring to fig. 3a and 3b, in some embodiments, the inner wall of the storage section 101 is provided with a mounting portion 104 that is convex or concave toward the inside of the storage section 101, and the partition 5 is mounted to the mounting portion 104. The mounting portion 104 may be annular or may be convex or concave only in a partial region of the inner wall circumference of the storage section 101. A mounting portion 104 is provided to facilitate mounting of the spacer 5.
The following describes a specific implementation of the reagent vessel holder 2.
Referring to fig. 4 and 5, in some embodiments, the reagent cartridge support 2 comprises a plate body 22 and a top cover 23. The board body 22 is detachably connected to the chip body 3. The top cover 23 is rotatably disposed at one end of the plate 22, and the top cover 23 is detachably connected to the chip body 3. Wherein the top cover 23 is positioned outside the first sealing member 12 of the reagent cartridge 1, and the second puncture needle 31 is positioned outside the second sealing member 13. The plate body 22, the top cover 23 and the chip body 3 jointly enclose the reagent cartridge 1.
The top cover 23 is movably and switchably attached to the plate body 22. The junction M of the top cover 23 and the plate body 22 may be made of a deformable material such as rubber, an elastic member, etc.; or a pivot or the like is adopted to realize the rotatable connection. Before the reagent cuvette 1 is not mounted, the top cover 23 is in the state shown in fig. 4 with respect to the plate body 22. After the reagent cartridge 1 is mounted in place, the top cover 23 is rotated by about 90 °, and the top cover 23 and the chip body 3 are snapped together.
Referring to fig. 4, in some embodiments, the top cover 23 includes a cover 231, a first catch 232, and a pusher 233. One side of the cover 231 is rotatably connected to the plate 22. The first clip 232 is disposed on a side of the top cover 23 away from the board 22 and configured to clip the chip body 3. The pusher 233 is configured in a ring shape, and the pusher 233 is mounted to the top cover 23. Wherein the first lancet 21 is mounted inside the pusher 233.
The lid 231 is constructed in a rectangular open lid-like structure, and the lid 231 serves as an outer frame of the top cover 23. The propeller 233 is disposed inside the outer frame, and the propeller 233 and the top cover 23 may be connected together by a connection rib. Mover 233 is generally a frame-like structure or a semi-enclosed structure of a plurality of plates. In the embodiment illustrated in fig. 4, the propeller 233 is a rectangular annular wall. Inside the area enclosed by the pusher 233, the second lancet 31 is disposed. Second lancet 31 may also be connected to pusher 233 by a rib. The number of second lancet 31 matches the number of micro-flow channels 331 on plate body 22. The first clip 232 is a protrusion disposed on the edge of the cover 231, and is clipped into the inner recess 322 of the chip body 3, so as to detachably connect the top cover 23 and the chip body 3.
Referring to fig. 4, the board body 22 is provided with a second catch 221, and the second catch 221 is configured to catch the chip body 3. The second buckle 221 buckles two sides of the edge of the chip body 3 to realize the detachable connection of the board body 22 and the chip body 3.
Referring to FIG. 4, to improve the puncturing effect of first lancet 21, in some embodiments, the outer wall of the lancet is configured with a pointed end through which puncturing of first seal 12 is accomplished. Specifically, in the cross-sectional direction: the outer wall of the first lancet 21 is configured as a quadrangle, a trilateral, a polygon, or other irregular shape.
The following describes a specific implementation of the chip body 3.
Referring to fig. 5 and 6, in some embodiments, the chip body 3 includes a connection portion 32 and a microfluidic portion 33. The connecting portion 32 is provided with a third catch 321 and an inner recess 322, the third catch 321 is engaged with the second catch 221, and the first catch 232 is inserted into the inner recess 322. The microfluidic part 33 is provided with a microfluidic channel 331, and the second lancet 31 communicates with the microfluidic channel 331. The arrangement of the microfluidic channels 331 may be configured as desired. The second lancet 31 is hollow and the second lancet 31 is located upstream of the microfluidic channel 331, and reagent flows from the reagent chamber 1 to the microfluidic channel 331 through the second lancet 31.
Referring to fig. 1 and 5, in some embodiments, at least one of the first and second lancet 21, 31 is arranged in a row. The first puncture needle 21 and the second puncture needle 31 may be hollow needles, i.e., the wall of the needle body performs a puncturing function, and the inner area of the needle body is hollow to perform a fluid circulating function.
The following describes the manufacturing and using process of the microfluidic chip assembly, taking the microfluidic chip assembly with the sealing ring 4 as an example, that is, the microfluidic chip assembly is suitable for liquid reagents.
Referring to fig. 2, first, a reagent cartridge 1 is prepared. The second sealing element 13 positioned at the most downstream is fixed in a hot pressing mode, and the bottom of the reagent cabin 1 is preliminarily sealed. Next, the seal ring 4 is installed; then, the second seal member 13 located upstream of the seal ring 4 is fixed by a hot press. Taking the second sealing member 13 as two layers as an example, a structure in which the sealing ring 4 is located in the middle and two second sealing members 13 are located on two sides is finally formed, that is, a structure of the second sealing member 13-the sealing ring 4-the second sealing member 13.
Secondly, according to different requirements of biochemical reaction, corresponding reagents are selected. The liquid reagent may be injected directly into the chamber. The chamber reserved for the sample can also be provided with a filtering device in advance according to the requirement, so as to help purify the sample to be detected. After the reagent is filled, the first sealing element 12 is fixed in a hot-press sealing mode to realize complete sealing of the reagent bin 1, so that the storage and transportation of the microfluidic chip assembly are facilitated. It should be additionally noted that, in the case of a solid reagent, the immobilized reagent is placed downstream of the storage section 101, and then the desiccant is filled in the upstream section of the partition 5, so as to keep the chamber dry for a long time and prolong the storage time of the solid reagent.
When needing to examine, at first, operating personnel installs reagent storehouse support 2, is in the same place the plate body 22 of reagent storehouse support 2 and chip body 3 lock joint to form the space that can hold reagent storehouse 1.
Then, according to the requirement of detection, selecting a corresponding reagent chamber 1, after a sample is extracted and injected into the sample cavity, placing the reagent chamber 1 between the plate body 22 and the chip body 3 in the correct orientation shown in fig. 1, and then fastening the top cover 23 of the reagent chamber support 2. In the embodiment, for nucleic acid detection, a reagent chamber 1 with eleven storage chambers 10 is selected, wherein ten storage chambers 10 are pre-packaged with detection reagents, and one sample chamber is reserved for loading a sample to be detected.
Then, the preliminarily assembled chip is put into a detection instrument, and the detection instrument is started. The detection instrument outputs a certain pressure, and the top cover 23 is pressed down to press the propeller 233 away from the cover 231 of the top cover 23. Along with the increase of the downward pressure, the pusher 233 will press the reagent chamber 1 to increase the contact force with the second puncture needle 31, so as to puncture the double-layer film of the lower sealing area of the reagent chamber 1 until the second puncture needle 31 and the sealing ring 4 realize interference fit to ensure that no leakage occurs.
After the interference fit between the reagent chamber 1 and the second puncture needle 31 of the chip body 3 is completed, the stroke of the pressure output surface of the instrument is increased again, the interior of the propeller 233 is squeezed, the first puncture needle 21 is pressed away from the propeller 233, and the first sealing element 12 of the reagent chamber 1 is punctured, so that the communication with the atmosphere is realized. The reagent can be normally released after being communicated, and can be taken by a control instrument according to the reaction requirement. The whole process needs to keep down the pressure, maintains interference fit and prevents leakage pollution.
And finally, removing the pressing force after the detection is finished, taking out the chip, and assembling the next chip to perform a new group of detection.
The microfluidic chip assembly provided by the technical scheme is convenient and fast to operate, has low requirements on operators, and only needs to install the reagent bin bracket 2, put the reagent bin 1 pre-loaded with the corresponding biochemical reaction reagent into the microfluidic chip assembly, close the top cover 23 and put the instrument into the instrument, and output a certain pressure by the instrument to communicate the reagent bin 1 with the chip body 3, so that preparation is made for the next detection work.
In the description of the present invention, it is to be understood that the terms "central", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be considered as limiting the scope of the present invention.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: it is to be understood that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof, but such modifications or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (13)
1. A microfluidic chip assembly, comprising:
the reagent cabin (1) comprises a cabin body (11), a first sealing element (12) and a second sealing element (13); the bin body (11) is provided with a storage cavity (10), one end of the bin body (11) is provided with the first sealing element (12), and the other end of the bin body (11) is provided with more than two layers of the second sealing elements (13);
a reagent cartridge holder (2) having a first piercing needle (21); and
a chip body (3) having a second puncture needle (31); the chip body (3) is detachably connected with the reagent bin support (2), and the chip body (3) and the reagent bin support (2) form a placing area for accommodating the reagent bin (1);
wherein the first lancet (21) corresponds to the first seal (12) and the second lancet (31) corresponds to the second seal (13).
2. Microfluidic chip assembly according to claim 1, wherein the storage chamber (10) is divided into a storage section (101), a narrow section (102) and a sealing section (103) along the axial direction of the reagent cartridge (1); the narrow section (102) has an inner diameter dimension that is less than the respective inner diameter dimensions of the storage section (101) and the sealing section (103).
3. Microfluidic chip assembly according to claim 2, wherein the second seals (13) are located at the sealing section (103) and each of the second seals (13) is arranged dispersed along the axial direction of the reagent cartridge (1).
4. The microfluidic chip assembly according to claim 3, wherein the sealing section (103) comprises a transition section (103 a) and a mounting section (103 b); the inner diameter of the transition section (103 a) is smaller than that of the mounting section (103 b); the transition section (103 a) is connected to the throat section (102).
5. The microfluidic chip assembly of claim 2, further comprising:
a sealing ring (4) disposed at a mounting section (103 b) of the sealing section (103), the sealing ring (4) being configured to seal against an outer wall of a second lancet (31) that punctures the second sealing member (13).
6. Microfluidic chip assembly according to claim 5, wherein at least one of the second seals (13) is located upstream of the sealing ring (4) and the remaining second seals (13) are located downstream of the sealing ring (4).
7. The microfluidic chip assembly of claim 2, further comprising:
a partition (5) provided inside the storage section (101) to divide the storage section (101) into a plurality of regions; the partition (5) is provided with a through hole (50) therethrough to communicate the respective regions.
8. Microfluidic chip assembly according to claim 7, wherein the inner wall of the storage section (101) is provided with a mounting portion (104) that is convex or concave towards the inside of the storage section (101), the partition (5) being mounted to the mounting portion (104).
9. Microfluidic chip assembly according to claim 1, wherein the reagent cartridge holder (2) comprises:
the plate body (22) is detachably connected with the chip body (3); and
the top cover (23) is rotatably arranged at one end of the plate body (22) and is detachably connected with the chip body (3);
wherein the top cover (23) is positioned outside the first seal (12) of the reagent cartridge (1), and the second lancet (31) is positioned outside the second seal (13); the plate body (22), the top cover (23) and the chip body (3) jointly wrap the reagent cabin (1).
10. The microfluidic chip assembly according to claim 9, wherein the top cover (23) comprises a cover (231), a first snap (232), and a pusher (233); one side of the cover body (231) is rotatably connected with the plate body (22); the first buckle (232) is arranged on one side of the top cover (23) far away from the plate body (22) and is configured to buckle the chip body (3); the pusher (233) is configured to be annular, mounted to the top cover (23); wherein the first lancet (21) is mounted inside the pusher (233); and/or the presence of a gas in the gas,
the plate body (22) is provided with a second catch (221), the second catch (221) being configured to catch the chip body (3).
11. Microfluidic chip assembly according to claim 10, wherein the chip body (3) comprises:
a connecting part (32) provided with a third buckle (321) and an inner concave part (322), wherein the third buckle (321) is connected with the second buckle (221) in a clamping way, and the first buckle (232) is inserted into the inner concave part (322); and
the microfluidic part (33) is provided with a microfluidic channel (331), and the second puncture needle (31) is communicated with the microfluidic channel (331).
12. Microfluidic chip assembly according to claim 1, wherein at least one of the first (21) and second (31) lancet is arranged in a row.
13. Microfluidic chip assembly according to claim 1, wherein at least one of the first seal (12) and the second seal (13) comprises a sealing film.
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| CN202110418205.1A CN115212932B (en) | 2021-04-19 | 2021-04-19 | Microfluidic chip assembly |
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| CN202110418205.1A CN115212932B (en) | 2021-04-19 | 2021-04-19 | Microfluidic chip assembly |
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| US20060263914A1 (en) * | 2005-05-19 | 2006-11-23 | Konica Minolta Medical & Graphic, Inc. | Testing chip and micro integrated analysis system |
| CN102454794A (en) * | 2010-10-20 | 2012-05-16 | 株式会社爱德克斯 | Liquid seal, shaft seal apparatus and pump apparatus using the same |
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| CN115212932B (en) | 2024-09-24 |
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