CN115212932A - Microfluidic chip components - Google Patents

Microfluidic chip components Download PDF

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Publication number
CN115212932A
CN115212932A CN202110418205.1A CN202110418205A CN115212932A CN 115212932 A CN115212932 A CN 115212932A CN 202110418205 A CN202110418205 A CN 202110418205A CN 115212932 A CN115212932 A CN 115212932A
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reagent
section
sealing
microfluidic chip
chip assembly
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CN115212932B (en
Inventor
张东旭
高润鑫
钟鑫泉
杜曼殊
孔志博
张剑滨
曾俊添
葛胜祥
张军
夏宁邵
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Xiamen Innodx Biotech Co ltd
Xiamen University
Beijing WanTai Biological Pharmacy Enterprise Co Ltd
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Xiamen Innodx Biotech Co ltd
Xiamen University
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0689Sealing

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

The invention discloses a microfluidic chip assembly, relates to the technical field of biological assay or inspection, and is used for optimizing the performance of the microfluidic chip assembly. The microfluidic chip assembly comprises a reagent bin, a reagent bin bracket and a chip body. The reagent cabin comprises a cabin body, a first sealing element and a second sealing element; the storehouse body has the storage chamber, and the one end of the storehouse body is provided with first sealing member, and the other end of the storehouse body is provided with the second sealing member more than two-layer. The reagent cartridge support has a first puncture needle. The chip body is provided with a second puncture needle; the chip body can be dismantled with reagent storehouse support and be connected, and chip body and reagent storehouse support form and place the region to hold the reagent storehouse. Wherein the first lancet corresponds to the first seal and the second lancet corresponds to the second seal. The microfluidic chip assembly provided by the technical scheme has good sealing performance, reduces the possibility of substance leakage in the storage cavity of the reagent bin, and improves the use reliability of the microfluidic chip assembly.

Description

微流控芯片组件Microfluidic chip components

技术领域technical field

本发明涉及生物测定或检验技术领域,具体涉及一种微流控芯片组件。The invention relates to the technical field of biological assay or inspection, in particular to a microfluidic chip assembly.

背景技术Background technique

核酸检测是目前传染病诊断的金标准之一。传统核酸检测方法步骤繁琐,对检测场地和操作人员资质均有较高要求。微流控芯片又称为芯片实验室,基于微流控芯片技术能够实现高集成、一体化的POCT(point-of-care test),大大缩短检测所需时间,实现随到随检。Nucleic acid testing is currently one of the gold standards for infectious disease diagnosis. The traditional nucleic acid detection methods are complicated in steps, and have high requirements on the testing site and the qualifications of operators. Microfluidic chip, also known as lab-on-a-chip, can realize highly integrated and integrated POCT (point-of-care test) based on microfluidic chip technology, greatly shorten the time required for detection, and realize on-demand inspection.

微流控芯片具有多个微小尺寸的流道、腔体且这些流道以一定规则排列,测试过程中,不同的生物试剂按照一定的顺序释放,并通过不同流道流动至指定腔体,完成各种生化反应,最终实现核酸的快速、准确检测。The microfluidic chip has multiple micro-sized flow channels and cavities, and these flow channels are arranged in a certain rule. During the test process, different biological reagents are released in a certain order, and flow to the designated cavity through different flow channels to complete the test. Various biochemical reactions, and finally realize the rapid and accurate detection of nucleic acid.

发明内容SUMMARY OF THE INVENTION

本发明实施例提供了一种微流控芯片组件,包括:An embodiment of the present invention provides a microfluidic chip assembly, including:

试剂仓,包括仓体、第一密封件以及第二密封件;所述仓体具有存储腔,所述仓体的一端设置有所述第一密封件,所述仓体的另一端设置有两层以上的所述第二密封件;The reagent chamber includes a chamber body, a first sealing member and a second sealing member; the chamber body has a storage cavity, one end of the chamber body is provided with the first sealing member, and the other end of the chamber body is provided with two the second seal above the layer;

试剂仓支架,具有第一刺破针;以及a reagent cartridge holder having a first puncture needle; and

芯片本体,具有第二刺破针;所述芯片本体与所述试剂仓支架可拆卸连接,且所述芯片本体和所述试剂仓支架形成放置区域,以容纳所述试剂仓;The chip body is provided with a second puncturing needle; the chip body is detachably connected to the reagent chamber support, and the chip body and the reagent chamber support form a placement area to accommodate the reagent chamber;

其中,所述第一刺破针对应于所述第一密封件,所述第二刺破针对应于所述第二密封件。Wherein, the first piercing pair corresponds to the first sealing member, and the second piercing pair corresponds to the second sealing member.

在一些实施例中,沿着所述试剂仓的轴线方向,所述存储腔被分为存储段、狭窄段以及密封段;所述狭窄段的内径尺寸小于所述存储段和所述密封段各自的内径尺寸。In some embodiments, along the axial direction of the reagent cartridge, the storage cavity is divided into a storage section, a narrow section and a sealing section; the inner diameter of the narrow section is smaller than the storage section and the sealing section respectively inner diameter size.

在一些实施例中,所述第二密封件位于所述密封段,且各个所述第二密封件沿着所述试剂仓的轴线方向分散设置。In some embodiments, the second sealing members are located in the sealing section, and each of the second sealing members is distributed along the axial direction of the reagent cartridge.

在一些实施例中,所述密封段包括过渡段以及安装段;所述过渡段的内径尺寸小于所述安装段的内径尺寸;所述过渡段与所述狭窄段连接。In some embodiments, the sealing section includes a transition section and an installation section; the inner diameter of the transition section is smaller than the inner diameter of the installation section; the transition section is connected to the narrow section.

在一些实施例中,微流控芯片组件还包括:In some embodiments, the microfluidic chip assembly further includes:

密封圈,设置于所述密封段的安装段,所述密封圈被构造为与刺破了所述第二密封件的第二刺破针的外壁抵顶密封。The sealing ring is arranged on the installation section of the sealing section, and the sealing ring is configured to abut and seal against the outer wall of the second puncturing needle that has pierced the second sealing member.

在一些实施例中,至少其中一个所述第二密封件位于所述密封圈的上游,其余的所述第二密封件位于所述密封圈的下游。In some embodiments, at least one of the second seals is located upstream of the seal ring and the remainder of the second seals are located downstream of the seal ring.

在一些实施例中,微流控芯片组件还包括:In some embodiments, the microfluidic chip assembly further includes:

分隔件,设置于所述存储段内部,以将所述存储段分为多个区域;所述分隔件设置有贯穿的通孔,以将各个所述区域连通。A partition is arranged inside the storage section to divide the storage section into a plurality of regions; the partition is provided with a through hole to connect each of the regions.

在一些实施例中,所述存储段的内壁设置有朝着所述存储段内部凸起或者内凹的安装部,所述分隔件安装于所述安装部。In some embodiments, the inner wall of the storage segment is provided with a mounting portion that is convex or concave toward the interior of the storage segment, and the partition is mounted to the mounting portion.

在一些实施例中,所述试剂仓支架包括:In some embodiments, the reagent cartridge holder includes:

板体,与所述芯片本体可拆卸连接;以及a plate body, which is detachably connected to the chip body; and

顶盖,可转动地设置于所述板体的一端,与所述芯片本体可拆卸连接;a top cover, which is rotatably arranged on one end of the plate body and is detachably connected to the chip body;

其中,所述顶盖位于所述试剂仓的第一密封件的外侧,所述第二刺破针位于所述第二密封件的外侧;所述板体、所述顶盖和所述芯片本体共同包住所述试剂仓。Wherein, the top cover is located on the outer side of the first sealing member of the reagent chamber, and the second puncturing needle is located on the outer side of the second sealing member; the plate body, the top cover and the chip body Co-encapsulate the reagent compartment.

在一些实施例中,所述顶盖包括盖体、第一卡扣和推进器;所述盖体一侧与所述板体可转动连接;所述第一卡扣设置于所述顶盖远离所述板体的一侧,且被构造为扣住所述芯片本体;所述推进器被构造为环形的,安装于所述顶盖;其中,所述第一刺破针安装于所述推进器的内部;和/或,In some embodiments, the top cover includes a cover body, a first buckle and a pusher; one side of the cover body is rotatably connected to the plate body; the first buckle is disposed away from the top cover one side of the plate body is configured to buckle the chip body; the pusher is configured in an annular shape and is mounted on the top cover; wherein, the first puncturing needle is mounted on the pusher internal; and/or,

所述板体设置有第二卡扣,所述第二卡扣被构造为扣住所述芯片本体。The plate body is provided with a second buckle, and the second buckle is configured to buckle the chip body.

在一些实施例中,所述芯片本体包括:In some embodiments, the chip body includes:

连接部,设置有第三卡扣以及内凹部,所述第三卡扣与所述第二卡扣卡合连接,所述第一卡扣插入所述内凹部;以及a connection part, which is provided with a third buckle and an inner recess, the third buckle is connected with the second buckle, and the first buckle is inserted into the inner recess; and

微流控部,设置有微流通道,所述第二刺破针与所述微流通道连通。The microfluidic part is provided with a microfluidic channel, and the second puncturing needle is communicated with the microfluidic channel.

在一些实施例中,所述第一刺破针和所述第二刺破针至少其中之一成排设置。In some embodiments, at least one of the first lancing needle and the second lancing needle is arranged in a row.

在一些实施例中,第一密封件和所述第二密封件至少其中之一包括密封膜。In some embodiments, at least one of the first seal and the second seal includes a sealing membrane.

上述技术方案提供的微流控芯片组件,在试剂仓的另一端设置有两层以上的第二密封件,该多层的第二密封件与芯片本体的第二刺破针配合,在芯片本体的第二刺破针刺破第二密封件后,由于第二密封件具有两层,所以可以起到双层密封作用,减少试剂仓的存储腔中物质泄漏的可能性,提高微流控芯片组件的使用可靠性。The microfluidic chip assembly provided by the above technical solution is provided with more than two layers of second sealing members at the other end of the reagent chamber. After the second puncturing needle pierces the second sealing member, since the second sealing member has two layers, it can play a double-layer sealing effect, reduce the possibility of material leakage in the storage cavity of the reagent chamber, and improve the microfluidic chip. reliability of components.

附图说明Description of drawings

此处所说明的附图用来提供对本发明的进一步理解,构成本申请的一部分,本发明的示意性实施例及其说明用于解释本发明,并不构成对本发明的不当限定。在附图中:The accompanying drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the attached image:

图1为本发明实施例提供的微流控芯片组件的分解结构示意图;1 is a schematic diagram of an exploded structure of a microfluidic chip assembly provided by an embodiment of the present invention;

图2为本发明实施例提供的微流控芯片组件的试剂仓结构示意图;FIG. 2 is a schematic structural diagram of a reagent compartment of a microfluidic chip assembly provided by an embodiment of the present invention;

图3a为本发明另一些实施例提供的微流控芯片组件的试剂仓结构示意图;Figure 3a is a schematic structural diagram of a reagent chamber of a microfluidic chip assembly provided by other embodiments of the present invention;

图3b为本发明另一些实施例提供的微流控芯片组件的分隔件结构示意图;3b is a schematic structural diagram of a separator of a microfluidic chip assembly provided by other embodiments of the present invention;

图4为本发明实施例提供的微流控芯片组件的试剂仓支架结构示意图;FIG. 4 is a schematic structural diagram of a reagent cartridge bracket of a microfluidic chip assembly provided by an embodiment of the present invention;

图5为本发明实施例提供的微流控芯片组件的芯片本体局部立体结构示意图;5 is a schematic diagram of a partial three-dimensional structure of a chip body of a microfluidic chip assembly provided by an embodiment of the present invention;

图6为本发明实施例提供的微流控芯片组件的芯片本体主视结构示意图。FIG. 6 is a schematic structural diagram of a front view of a chip body of a microfluidic chip assembly provided by an embodiment of the present invention.

具体实施方式Detailed ways

下面结合图1~图6对本发明提供的技术方案进行更为详细的阐述。The technical solutions provided by the present invention will be described in more detail below with reference to FIGS. 1 to 6 .

参见图1,本发明实施例提供一种微流控芯片组件,包括试剂仓1、试剂仓支架2以及芯片本体3。参见图2,试剂仓1包括仓体11、第一密封件12以及第二密封件13。仓体11具有存储腔10,仓体11的一端设置有第一密封件12,仓体11的另一端设置有两层以上的第二密封件13。参见图4,试剂仓支架2具有第一刺破针21。参见图1、图5和图6,芯片本体3具有第二刺破针31;芯片本体3与试剂仓支架2可拆卸连接,且芯片本体3和试剂仓支架2形成放置区域,以容纳试剂仓1。其中,第一刺破针21对应于第一密封件12,第二刺破针31对应于第二密封件13。Referring to FIG. 1 , an embodiment of the present invention provides a microfluidic chip assembly, including a reagent chamber 1 , a reagent chamber support 2 and a chip body 3 . Referring to FIG. 2 , the reagent cartridge 1 includes a cartridge body 11 , a first sealing member 12 and a second sealing member 13 . The bin body 11 has a storage cavity 10 , one end of the bin body 11 is provided with a first sealing member 12 , and the other end of the bin body 11 is provided with two or more layers of second sealing members 13 . Referring to FIG. 4 , the reagent cartridge holder 2 has a first puncturing needle 21 . 1 , 5 and 6 , the chip body 3 has a second puncturing needle 31 ; the chip body 3 is detachably connected to the reagent chamber support 2 , and the chip body 3 and the reagent chamber support 2 form a placement area to accommodate the reagent chamber 1. The first puncturing needle 21 corresponds to the first sealing member 12 , and the second puncturing needle 31 corresponds to the second sealing member 13 .

参见图1,试剂仓支架2和芯片本体3配合,为试剂仓1提供支撑和固定,为试剂仓1沿图1所示的竖直方向运动提供约束,使得试剂仓1不会掉落,使得微流控芯片组件在未使用状态和使用状态下,试剂仓1均能有效固定。未使用状态是指第一密封件12和第二密封件13在未刺破之前的状态;使用状态是指使用微流控芯片组件做测试的状态,即将第一密封件12和第二密封件13刺破,以释放试剂仓1的存储腔10中的试剂。Referring to FIG. 1 , the reagent compartment bracket 2 cooperates with the chip body 3 to provide support and fixation for the reagent compartment 1, and to provide constraints for the reagent compartment 1 to move in the vertical direction shown in FIG. The reagent chamber 1 can be effectively fixed in both the unused state and the used state of the microfluidic chip assembly. The unused state refers to the state of the first seal 12 and the second seal 13 before they are punctured; the used state refers to the state of using the microfluidic chip assembly for testing, that is, the first seal 12 and the second seal 13 to puncture to release the reagent in the storage chamber 10 of the reagent cartridge 1.

第一刺破针21对应于第一密封件12,是指微流控芯片组件的各个部件安装到位后,第一刺破针21位于第一密封件12附近,以图1所示的方向为例,第一刺破针21位于第一密封件12的上方;后续需要使用时,通过改变第一刺破针21和第一密封件12之间的距离,使得第一刺破针21将第一密封件12刺破。The first puncturing needle 21 corresponds to the first sealing member 12, which means that after each component of the microfluidic chip assembly is installed in place, the first puncturing needle 21 is located near the first sealing member 12, and the direction shown in FIG. 1 is For example, the first puncturing needle 21 is located above the first sealing member 12; when subsequent use is required, by changing the distance between the first puncturing needle 21 and the first sealing member 12, the first puncturing needle 21 will A seal 12 is pierced.

第二刺破针31对应于第二密封件13,是指微流控芯片组件的各个部件安装到位后,第二刺破针31位于第二密封件13附近,以图1所示的方向为例,第二刺破针31位于第二密封件13的下方。后续需要使用时,通过改变第二刺破针31和第二密封件13之间的距离,使得第二刺破针31将第二密封件13刺破。The second puncturing needle 31 corresponds to the second sealing member 13 , which means that after each component of the microfluidic chip assembly is installed in place, the second puncturing needle 31 is located near the second sealing member 13 , and the direction shown in FIG. 1 is For example, the second puncturing needle 31 is located below the second sealing member 13 . When subsequent use is required, the second puncturing needle 31 can pierce the second sealing member 13 by changing the distance between the second puncturing needle 31 and the second sealing member 13 .

试剂仓1的存储腔10用于存储液体试剂或者冻干试剂。试剂仓1的数据根据芯片本体3的第二刺破针31的数量确定,可以根据生化反应的流程需求灵活增减。试剂仓1成排设置,每个试剂仓1对应设置一个第一刺破针21和一个第二刺破针31。所有的试剂仓1固定在一起,形成试剂仓整体。The storage chamber 10 of the reagent chamber 1 is used for storing liquid reagents or lyophilized reagents. The data of the reagent chamber 1 is determined according to the number of the second puncturing needles 31 of the chip body 3 , and can be flexibly increased or decreased according to the flow requirements of the biochemical reaction. The reagent chambers 1 are arranged in rows, and each reagent chamber 1 is correspondingly provided with a first puncturing needle 21 and a second puncturing needle 31 . All the reagent chambers 1 are fixed together to form the whole reagent chamber.

试剂仓1的第一密封件12、各第二密封件13实现了释放存储腔10中试剂过程中的全程密闭。各第二密封件13采用热压密封实现安装,第一密封件12是在试剂装载完毕后进行热压密封。The first sealing member 12 and each second sealing member 13 of the reagent chamber 1 realize the whole process of sealing in the process of releasing the reagent in the storage chamber 10 . Each second sealing member 13 is installed by heat-press sealing, and the first sealing member 12 is heat-pressed and sealed after the reagent is loaded.

参见图2,在一些实施例中,沿着试剂仓1的轴线方向,即试剂仓1的长度方向,存储腔10被分为存储段101、狭窄段102以及密封段103。狭窄段102位于存储段101和密封段103之间。狭窄段102的内径尺寸小于存储段101和密封段103各自的内径尺寸。Referring to FIG. 2 , in some embodiments, the storage cavity 10 is divided into a storage section 101 , a narrow section 102 and a sealing section 103 along the axial direction of the reagent container 1 , that is, the length direction of the reagent container 1 . The narrow section 102 is located between the storage section 101 and the sealing section 103 . The inner diameter of the narrow section 102 is smaller than the respective inner diameters of the storage section 101 and the sealing section 103 .

存储段101的内径尺寸被构造为是渐变的,存储段101朝向狭窄段102的一侧的内径尺寸小,存储段101远离狭窄段102的内径尺寸大,以方便后续释放试剂。The inner diameter of the storage section 101 is configured to be gradual, the inner diameter of the side of the storage section 101 facing the narrow section 102 is small, and the inner diameter of the storage section 101 away from the narrow section 102 is large, so as to facilitate subsequent release of the reagent.

狭窄段102的内径尺寸比较小,在需要的时候,可以将狭窄段102的内径尺寸设置的比较小,以使得狭窄段102与刺破了第二密封件13的第二刺破针31的外壁之间具有小缝隙。The inner diameter of the narrow section 102 is relatively small, and when necessary, the inner diameter of the narrow section 102 can be set relatively small, so that the narrow section 102 and the outer wall of the second puncturing needle 31 that has pierced the second seal 13 There are small gaps in between.

参见图2,在一些实施例中,第二密封件13位于密封段103,且各个第二密封件13沿着试剂仓1的轴线方向分散设置。第二密封件13比如采用片状结构,各个第二密封件13单独安装,共同起到多重密封的作用。Referring to FIG. 2 , in some embodiments, the second sealing members 13 are located in the sealing section 103 , and each second sealing member 13 is distributed along the axial direction of the reagent cartridge 1 . For example, the second sealing members 13 adopt a sheet-like structure, and each of the second sealing members 13 is installed separately to jointly play the role of multiple sealing.

继续参见图2,在一些实施例中,密封段103包括过渡段103a以及安装段103b。过渡段103a的内径尺寸小于安装段103b的内径尺寸。过渡段103a位于狭窄段102和安装段103b之间。过渡段103a与狭窄段102连接。过渡段103a和安装段103b的内径尺寸均大于狭窄段102的内径尺寸。Continuing to refer to FIG. 2, in some embodiments, the sealing section 103 includes a transition section 103a and a mounting section 103b. The inner diameter of the transition section 103a is smaller than the inner diameter of the mounting section 103b. The transition section 103a is located between the narrow section 102 and the mounting section 103b. The transition section 103a is connected to the narrow section 102 . The inner diameter of the transition section 103 a and the installation section 103 b are both larger than the inner diameter of the narrow section 102 .

继续参见图2,在一些实施例中,微流控芯片组件还包括密封圈4,密封圈4设置于密封段103的安装段103b,密封圈4被构造为与刺破了第二密封件13的第二刺破针31的外壁抵顶密封。密封圈4比如为橡胶材质的。Continuing to refer to FIG. 2 , in some embodiments, the microfluidic chip assembly further includes a sealing ring 4 , the sealing ring 4 is disposed on the mounting section 103 b of the sealing section 103 , and the sealing ring 4 is configured to pierce the second sealing member 13 . The outer wall of the second puncturing needle 31 abuts against the seal. The sealing ring 4 is made of rubber, for example.

带有密封圈4的微流控芯片组件,尤其适用于液体试剂。在运输过程中,或者其他试剂仓1端部的第一密封件12和第二密封件13都未被刺破的情况下,通过试剂仓1顶部的第一密封件12以及底部的多层第二密封件13,就能对液体试剂起到比较好的密封作用。Microfluidic chip assembly with sealing ring 4, especially suitable for liquid reagents. During transportation, or when the first sealing member 12 and the second sealing member 13 at the end of the reagent container 1 are not pierced, the first sealing member 12 at the top of the reagent container 1 and the multi-layer sealing member 13 at the bottom of the reagent container The second sealing member 13 can effectively seal the liquid reagent.

在使用微流控芯片组件的过程中,对试剂仓支架2施加向下的力即朝着第二刺破针31方向的力,采用芯片本体3的第二刺破针31刺破试剂仓1底部的第二密封件13,此时,第二刺破针31的外壁会被密封圈4严密包裹,以防止泄露;然后,继续对试剂仓支架2施加向下的力,试剂仓支架2的第一刺破针21刺破试剂仓1顶部的第一密封件12,使得试剂仓1的存储腔10与外部大气连通,以实现试剂的释放。此时,由于密封圈4与芯片本体3的第二刺破针31之间的密封连接,使得存储腔10内的液体不会出现泄露。In the process of using the microfluidic chip assembly, a downward force is applied to the reagent cartridge holder 2 , that is, a force in the direction of the second puncturing needle 31 , and the second puncturing needle 31 of the chip body 3 is used to puncture the reagent cartridge 1 The second sealing member 13 at the bottom, at this time, the outer wall of the second puncturing needle 31 will be tightly wrapped by the sealing ring 4 to prevent leakage; The first piercing needle 21 pierces the first sealing member 12 on the top of the reagent container 1, so that the storage cavity 10 of the reagent container 1 is communicated with the external atmosphere, so as to realize the release of the reagent. At this time, due to the sealed connection between the sealing ring 4 and the second puncturing needle 31 of the chip body 3, the liquid in the storage cavity 10 will not leak.

参见图2,在一些实施例中,至少其中一个第二密封件13位于密封圈4的上游,其余的第二密封件13位于密封圈4的下游。多个第二密封件13中的至少其中一个位于密封圈4的上游,其余的第二密封件13位于密封圈4的下游。Referring to FIG. 2 , in some embodiments, at least one of the second seals 13 is located upstream of the seal ring 4 , and the remaining second seals 13 are located downstream of the seal ring 4 . At least one of the plurality of second seals 13 is located upstream of the seal ring 4 , and the remaining second seals 13 are located downstream of the seal ring 4 .

设置密封圈4之后,由于位于密封圈4的上游的第二密封件13的密封作用,使得试剂仓1在存储过程中,防止密封圈4浸泡在试剂中从而降低密封圈4寿命,也避免了试剂与密封圈4发生反应,使试剂变质失效。After the sealing ring 4 is installed, due to the sealing effect of the second sealing member 13 located upstream of the sealing ring 4, the reagent chamber 1 prevents the sealing ring 4 from soaking in the reagent during the storage process, thereby reducing the life of the sealing ring 4, and avoiding the The reagent reacts with the sealing ring 4 to make the reagent deteriorate and fail.

可见,上述技术方案既改善了微流控芯片组件未使用状态下的密封性能,又改善了微流控芯片组件使用状态下的密封性能。It can be seen that the above technical solution not only improves the sealing performance of the microfluidic chip assembly in an unused state, but also improves the sealing performance of the microfluidic chip assembly in a used state.

参见图3a和图3b,在另一些实施例中,微流控芯片组件还包括分隔件5,分隔件5设置于存储段101内部,以将存储段101分为多个区域,每个区域被构造为放置一种生物材料。分隔件5设置有贯穿的通孔50,以将各个区域连通。Referring to FIGS. 3a and 3b, in other embodiments, the microfluidic chip assembly further includes a spacer 5, and the spacer 5 is disposed inside the storage segment 101 to divide the storage segment 101 into a plurality of regions, each region being Constructed to place a biological material. The spacer 5 is provided with through holes 50 therethrough to communicate the various regions.

如果适用于冻干试剂,微流控芯片组件可以不设置密封圈4。此时,采用分隔件5,将存储段101分为多个区段,如图3a和图3b所示,每个区段可以放置不同的冻干试剂。此时,试剂仓1底部的第二密封件13同样起到双层密封的作用,从而提高微流控芯片组件的密封性能,利于实现快速核酸现场检测。If it is suitable for lyophilized reagents, the microfluidic chip assembly may not be provided with the sealing ring 4 . At this time, the separator 5 is used to divide the storage section 101 into a plurality of sections, as shown in FIG. 3 a and FIG. 3 b , and different lyophilized reagents can be placed in each section. At this time, the second sealing member 13 at the bottom of the reagent chamber 1 also plays the role of double-layer sealing, so as to improve the sealing performance of the microfluidic chip assembly and facilitate the realization of rapid nucleic acid on-site detection.

当装载试剂冻干球的时候,位于分隔件5下游的区段容纳冻干球,位于分隔件5上游的区段容纳干燥剂,以延长冻干球的保存时间。When the reagent lyophilized spheres are loaded, the section located downstream of the partition 5 accommodates the lyophilized spheres, and the section located upstream of the partition 5 accommodates the desiccant, so as to prolong the storage time of the lyophilized spheres.

参见图3a和图3b,在一些实施例中,存储段101的内壁设置有朝着存储段101内部凸起或者内凹的安装部104,分隔件5安装于安装部104。安装部104可以为环形的,或者只在存储段101的内壁周向的部分区域凸起或者内凹。设置安装部104,便于安装分隔件5。Referring to FIGS. 3 a and 3 b , in some embodiments, the inner wall of the storage segment 101 is provided with a mounting portion 104 that is convex or concave toward the interior of the storage segment 101 , and the partition 5 is mounted to the mounting portion 104 . The mounting portion 104 may be annular, or only convex or concave in a partial area of the inner wall of the storage section 101 in the circumferential direction. The mounting portion 104 is provided to facilitate the mounting of the spacer 5 .

下面介绍试剂仓支架2的具体实现方式。The specific implementation of the reagent cartridge holder 2 is described below.

参见图4和图5,在一些实施例中,试剂仓支架2包括板体22以及顶盖23。板体22与芯片本体3可拆卸连接。顶盖23可转动地设置于板体22的一端,顶盖23与芯片本体3可拆卸连接。其中,顶盖23位于试剂仓1的第一密封件12的外侧,第二刺破针31位于第二密封件13的外侧。板体22、顶盖23和芯片本体3共同包住试剂仓1。Referring to FIGS. 4 and 5 , in some embodiments, the reagent cartridge holder 2 includes a plate body 22 and a top cover 23 . The plate body 22 is detachably connected to the chip body 3 . The top cover 23 is rotatably disposed on one end of the plate body 22 , and the top cover 23 is detachably connected to the chip body 3 . The top cover 23 is located outside the first sealing member 12 of the reagent chamber 1 , and the second puncturing needle 31 is located outside the second sealing member 13 . The plate body 22 , the top cover 23 and the chip body 3 enclose the reagent chamber 1 together.

顶盖23可动、可切换位置地安装于板体22。顶盖23和板体22的连接处M可以采用可变形材料,比如橡胶、弹性件等;或者采用枢轴等方式实现可转动连接。未安装试剂仓1之前,顶盖23相对于板体22是图4所示的状态。在试剂仓1安装到位后,将顶盖23转动约90°,将顶盖23与芯片本体3卡扣在一起。The top cover 23 is mounted on the plate body 22 in a movable and switchable position. The connection point M between the top cover 23 and the plate body 22 can be made of deformable materials, such as rubber, elastic parts, etc.; Before the reagent chamber 1 is not installed, the top cover 23 is in the state shown in FIG. 4 relative to the plate body 22 . After the reagent chamber 1 is installed in place, the top cover 23 is rotated about 90°, and the top cover 23 and the chip body 3 are snapped together.

参见图4,在一些实施例中,顶盖23包括盖体231、第一卡扣232和推进器233。盖体231一侧与板体22可转动连接。第一卡扣232设置于顶盖23远离板体22的一侧,且被构造为扣住所述芯片本体3。推进器233被构造为环形的,推进器233安装于顶盖23。其中,第一刺破针21安装于推进器233的内部。Referring to FIG. 4 , in some embodiments, the top cover 23 includes a cover body 231 , a first buckle 232 and a pusher 233 . One side of the cover body 231 is rotatably connected with the plate body 22 . The first buckle 232 is disposed on the side of the top cover 23 away from the plate body 22 and is configured to buckle the chip body 3 . The pusher 233 is configured in an annular shape, and the pusher 233 is mounted on the top cover 23 . The first puncturing needle 21 is installed inside the pusher 233 .

盖体231被构造为矩形的开口盖状结构,盖体231作为顶盖23的外框。在外框的内部设置有推进器233,具体可以通过连接筋条将推进器233与顶盖23连接在一起。推进器233大致为多块板围成的框状结构或者半包围结构。在图4所示意的实施例中,推进器233为矩形的环形壁体。在推进器233围成的区域内部,设置有第二刺破针31。第二刺破针31也可以通过筋条与推进器233连接。第二刺破针31的数量与板体22上的微流通道331的数量匹配。第一卡扣232为设置在盖体231边缘的凸起,通过卡入到芯片本体3的内凹部322中,实现顶盖23和芯片本体3的可拆卸连接。The cover body 231 is configured as a rectangular open cover-like structure, and the cover body 231 serves as an outer frame of the top cover 23 . A pusher 233 is provided inside the outer frame. Specifically, the pusher 233 and the top cover 23 can be connected together by connecting ribs. The propeller 233 is generally a frame-like structure or a semi-enclosed structure surrounded by a plurality of plates. In the embodiment shown in FIG. 4, the pusher 233 is a rectangular annular wall. Inside the area enclosed by the pusher 233, the second puncturing needle 31 is provided. The second puncturing needle 31 can also be connected to the pusher 233 through a rib. The number of the second puncturing needles 31 matches the number of the microfluidic channels 331 on the plate body 22 . The first snap 232 is a protrusion disposed on the edge of the cover body 231 , and is snapped into the concave portion 322 of the chip body 3 to realize detachable connection between the top cover 23 and the chip body 3 .

参见图4,板体22设置有第二卡扣221,第二卡扣221被构造为扣住芯片本体3。第二卡扣221扣住芯片本体3边缘的两侧,以实现板体22和芯片本体3的可拆卸连接。Referring to FIG. 4 , the board body 22 is provided with a second buckle 221 , and the second buckle 221 is configured to buckle the chip body 3 . The second buckles 221 buckle both sides of the edge of the chip body 3 to realize the detachable connection between the board body 22 and the chip body 3 .

参见图4,为了改善第一刺破针21的刺破效果,在一些实施例中,刺破针的外壁被构造为带有尖端,通过尖端实现对第一密封件12的刺破。具体来说,在横截面方向上:第一刺破针21的外壁被构造为四边形的、三边形的、多边形的、或者其他不规则的形状。Referring to FIG. 4 , in order to improve the piercing effect of the first puncturing needle 21 , in some embodiments, the outer wall of the puncturing needle is configured with a pointed end, and the first sealing member 12 can be pierced by the pointed end. Specifically, in the cross-sectional direction: the outer wall of the first puncturing needle 21 is configured in a quadrangular, triangular, polygonal, or other irregular shape.

下面介绍芯片本体3的具体实现方式。The specific implementation manner of the chip body 3 is described below.

参见图5和图6,在一些实施例中,芯片本体3包括连接部32以及微流控部33。连接部32设置有第三卡扣321以及内凹部322,第三卡扣321与第二卡扣221卡合连接,第一卡扣232插入内凹部322。微流控部33设置有微流通道331,第二刺破针31与微流通道331连通。微流通道331的布置方式可以根据需要进行设置。第二刺破针31是中空的,且第二刺破针31位于微流通道331的上游,试剂从试剂仓1经过第二刺破针31流向微流通道331。Referring to FIGS. 5 and 6 , in some embodiments, the chip body 3 includes a connection part 32 and a microfluidic part 33 . The connecting portion 32 is provided with a third buckle 321 and an inner concave portion 322 , the third buckle 321 is connected with the second buckle 221 by engaging, and the first buckle 232 is inserted into the inner concave portion 322 . The microfluidic part 33 is provided with a microfluidic channel 331 , and the second puncturing needle 31 communicates with the microfluidic channel 331 . The arrangement of the microfluidic channels 331 can be set as required. The second puncturing needle 31 is hollow, and the second puncturing needle 31 is located upstream of the microfluidic channel 331 , and the reagent flows from the reagent cartridge 1 through the second puncturing needle 31 to the microfluidic channel 331 .

参见图1和图5,在一些实施例中,第一刺破针21和第二刺破针31至少其中之一成排设置。第一刺破针21和第二刺破针31可以采用中空的针体,即针体的壁体起到刺破作用,针体的内部区域是中空的以起到流通流体的作用。Referring to FIGS. 1 and 5 , in some embodiments, at least one of the first puncturing needles 21 and the second puncturing needles 31 is arranged in a row. The first puncturing needle 21 and the second puncturing needle 31 may adopt hollow needle bodies, that is, the wall body of the needle body plays a puncturing function, and the inner area of the needle body is hollow to play the role of circulating fluid.

下面介绍微流控芯片组件的制造和使用过程,以微流控芯片组件带有密封圈4为例,即微流控芯片组件适用于液体试剂为例。The manufacturing and use process of the microfluidic chip assembly is described below, taking the microfluidic chip assembly with the sealing ring 4 as an example, that is, the microfluidic chip assembly is suitable for liquid reagents as an example.

参见图2,首先,制备试剂仓1。先通过热压方式固定位于最下游的第二密封件13,对试剂仓1底部进行初步密封。接下来,再安装密封圈4;然后,通过热压方式固定位于密封圈4上游的第二密封件13。以第二密封件13为两层为例,最终形成了密封圈4位于中间、两个第二密封件13位于两侧的结构,即第二密封件13-密封圈4-第二密封件13的结构。Referring to FIG. 2, first, the reagent cartridge 1 is prepared. First, the second sealing member 13 located at the most downstream is fixed by hot pressing, and the bottom of the reagent chamber 1 is preliminarily sealed. Next, the sealing ring 4 is installed again; then, the second sealing member 13 located upstream of the sealing ring 4 is fixed by hot pressing. Taking the second sealing member 13 as two layers as an example, a structure in which the sealing ring 4 is located in the middle and the two second sealing members 13 are located on both sides is finally formed, that is, the second sealing member 13 - the sealing ring 4 - the second sealing member 13 Structure.

其次,根据生化反应的不同需求,选择相应试剂。液体试剂可以直接注入腔室内。预留给样品的腔室也可以根据需求提前安装过滤装置,帮助纯化待测样品。试剂填充完毕后在采用热压密封的方式固定第一密封件12,以实现试剂仓1的完全密封,以便于微流控芯片组件的储存和运输。需要额外说明的是,如果是固体试剂,先在存储段101的下游放置固定试剂,然后在分隔件5的上游区段填充干燥剂,以保持腔室的长期干燥,延长固体试剂的储存时间。Secondly, according to the different needs of the biochemical reaction, select the corresponding reagents. Liquid reagents can be injected directly into the chamber. The chamber reserved for the sample can also be equipped with a filter device in advance as required to help purify the sample to be tested. After the reagents are filled, the first sealing member 12 is fixed by means of thermal pressure sealing, so as to realize the complete sealing of the reagent chamber 1, so as to facilitate the storage and transportation of the microfluidic chip assembly. It should be additionally explained that, if it is a solid reagent, the immobilization reagent is placed downstream of the storage section 101 first, and then the desiccant is filled in the upstream section of the partition 5 to keep the chamber dry for a long time and prolong the storage time of the solid reagent.

当需要进行检测时,首先,操作人员安装试剂仓支架2,将试剂仓支架2的板体22与芯片本体3扣合在一起,以形成可盛放试剂仓1的空间。When testing is required, first, the operator installs the reagent cartridge holder 2 , and fastens the plate body 22 of the reagent cartridge holder 2 with the chip body 3 to form a space that can hold the reagent cartridge 1 .

然后,根据检测的需求选取相应的试剂仓1,提取样品注入样品腔后,再将试剂仓1以图1所示的正确朝向放入板体22和芯片本体3之间,再扣上试剂仓支架2的顶盖23。本实施例针对核酸检测,选用了共有十一个存储腔10的试剂仓1,其中十个存储腔10预封装有检测试剂,一个样品腔预留以装载待测样品。Then, select the corresponding reagent chamber 1 according to the detection requirements, and after the extracted sample is injected into the sample chamber, the reagent chamber 1 is placed between the plate body 22 and the chip body 3 in the correct orientation shown in FIG. 1 , and then the reagent chamber is fastened. The top cover 23 of the bracket 2 . In this embodiment, for nucleic acid detection, a reagent chamber 1 with eleven storage chambers 10 is selected, wherein ten storage chambers 10 are pre-packaged with detection reagents, and one sample chamber is reserved for loading samples to be tested.

然后,将初步组装好的芯片放进检测仪器,启动检测仪器。检测仪器输出一定压力,先对顶盖23进行下压,将推进器233压离顶盖23的盖体231。随着下压力增加,推进器233会挤压试剂仓1使之与第二刺破针31的接触力增加,从而刺破试剂仓1下密封区的双层薄膜,直至第二刺破针31与密封圈4实现过盈配合确保不会泄露。Then, put the preliminarily assembled chip into the testing apparatus, and start the testing apparatus. The detection instrument outputs a certain pressure, and firstly, the top cover 23 is pressed down, and the pusher 233 is pressed away from the cover body 231 of the top cover 23 . As the downward pressure increases, the pusher 233 will press the reagent cartridge 1 to increase the contact force between the reagent cartridge 1 and the second puncturing needle 31 , thereby piercing the double-layer film in the lower sealing area of the reagent cartridge 1 until the second puncturing needle 31 An interference fit with the sealing ring 4 ensures no leakage.

完成试剂仓1与芯片本体3的第二刺破针31过盈配合后,仪器压力输出面的行程再次增加,挤压推进器233内部,将第一刺破针21压离推进器233,刺破试剂仓1的第一密封件12,实现与大气的连通。连通后即可正常释放试剂,可根据反应需要,通过控制仪器对试剂的按序取用。整个过程需要保持下压力,维持过盈配合防止泄露污染。After the interference fit between the reagent chamber 1 and the second puncture needle 31 of the chip body 3 is completed, the stroke of the pressure output surface of the instrument increases again, squeezes the inside of the pusher 233, and presses the first puncture needle 21 away from the pusher 233, puncturing the pusher 233. The first sealing member 12 of the reagent chamber 1 is broken to realize the communication with the atmosphere. After the connection, the reagents can be released normally, and the reagents can be taken in sequence by controlling the instrument according to the needs of the reaction. The whole process needs to maintain the down pressure and maintain the interference fit to prevent leakage and pollution.

最后,待检测结束可撤去压紧力,取出芯片,开始组装下一个芯片进行新一组的检测。Finally, when the test is over, the pressing force can be removed, the chip can be taken out, and the next chip can be assembled for a new set of tests.

上述技术方案提供的微流控芯片组件,操作便捷,对操作人员要求低,只需要安装好试剂仓支架2,再将预装有相应生化反应试剂的试剂仓1放入其中,合上顶盖23放入相对于仪器中,仪器输出一定压力,使试剂仓1与芯片本体3连通,这就为下一步的检测工作做好了准备。The microfluidic chip assembly provided by the above technical solution is easy to operate and has low requirements for operators. It only needs to install the reagent chamber bracket 2, and then put the reagent chamber 1 preloaded with the corresponding biochemical reaction reagents into it, and close the top cover. 23 is placed into the relative instrument, and the instrument outputs a certain pressure to make the reagent chamber 1 communicate with the chip body 3, which is ready for the next detection work.

在本发明的描述中,需要理解的是,术语“中心”、“纵向”、“横向”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系,仅是为便于描述本发明和简化描述,而不是指示或暗指所指的装置或元件必须具有特定的方位、为特定的方位构造和操作,因而不能理解为对本发明保护内容的限制。In the description of the present invention, it should be understood that the terms "center", "portrait", "horizontal", "front", "rear", "left", "right", "vertical", "horizontal", The orientation or positional relationship indicated by "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or obscuring It means that the referred device or element must have a specific orientation, be constructed and operate for a specific orientation, and therefore cannot be understood as a limitation on the protection content of the present invention.

最后应说明的是:以上实施例仅用以说明本发明的技术方案,而非对其限制;尽管参照前述实施例对本发明进行了详细的说明,本领域的普通技术人员应当理解:其依然可以对前述各实施例所记载的技术方案进行修改,或者对其中部分技术特征进行等同替换,但这些修改或者替换,并不使相应技术方案的本质脱离本发明各实施例技术方案的精神和范围。Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that it can still be Modifications are made to the technical solutions described in the foregoing embodiments, or some technical features thereof are equivalently replaced, but these modifications or replacements do not make the essence of the corresponding technical solutions depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (13)

1. A microfluidic chip assembly, comprising:
the reagent cabin (1) comprises a cabin body (11), a first sealing element (12) and a second sealing element (13); the bin body (11) is provided with a storage cavity (10), one end of the bin body (11) is provided with the first sealing element (12), and the other end of the bin body (11) is provided with more than two layers of the second sealing elements (13);
a reagent cartridge holder (2) having a first piercing needle (21); and
a chip body (3) having a second puncture needle (31); the chip body (3) is detachably connected with the reagent bin support (2), and the chip body (3) and the reagent bin support (2) form a placing area for accommodating the reagent bin (1);
wherein the first lancet (21) corresponds to the first seal (12) and the second lancet (31) corresponds to the second seal (13).
2. Microfluidic chip assembly according to claim 1, wherein the storage chamber (10) is divided into a storage section (101), a narrow section (102) and a sealing section (103) along the axial direction of the reagent cartridge (1); the narrow section (102) has an inner diameter dimension that is less than the respective inner diameter dimensions of the storage section (101) and the sealing section (103).
3. Microfluidic chip assembly according to claim 2, wherein the second seals (13) are located at the sealing section (103) and each of the second seals (13) is arranged dispersed along the axial direction of the reagent cartridge (1).
4. The microfluidic chip assembly according to claim 3, wherein the sealing section (103) comprises a transition section (103 a) and a mounting section (103 b); the inner diameter of the transition section (103 a) is smaller than that of the mounting section (103 b); the transition section (103 a) is connected to the throat section (102).
5. The microfluidic chip assembly of claim 2, further comprising:
a sealing ring (4) disposed at a mounting section (103 b) of the sealing section (103), the sealing ring (4) being configured to seal against an outer wall of a second lancet (31) that punctures the second sealing member (13).
6. Microfluidic chip assembly according to claim 5, wherein at least one of the second seals (13) is located upstream of the sealing ring (4) and the remaining second seals (13) are located downstream of the sealing ring (4).
7. The microfluidic chip assembly of claim 2, further comprising:
a partition (5) provided inside the storage section (101) to divide the storage section (101) into a plurality of regions; the partition (5) is provided with a through hole (50) therethrough to communicate the respective regions.
8. Microfluidic chip assembly according to claim 7, wherein the inner wall of the storage section (101) is provided with a mounting portion (104) that is convex or concave towards the inside of the storage section (101), the partition (5) being mounted to the mounting portion (104).
9. Microfluidic chip assembly according to claim 1, wherein the reagent cartridge holder (2) comprises:
the plate body (22) is detachably connected with the chip body (3); and
the top cover (23) is rotatably arranged at one end of the plate body (22) and is detachably connected with the chip body (3);
wherein the top cover (23) is positioned outside the first seal (12) of the reagent cartridge (1), and the second lancet (31) is positioned outside the second seal (13); the plate body (22), the top cover (23) and the chip body (3) jointly wrap the reagent cabin (1).
10. The microfluidic chip assembly according to claim 9, wherein the top cover (23) comprises a cover (231), a first snap (232), and a pusher (233); one side of the cover body (231) is rotatably connected with the plate body (22); the first buckle (232) is arranged on one side of the top cover (23) far away from the plate body (22) and is configured to buckle the chip body (3); the pusher (233) is configured to be annular, mounted to the top cover (23); wherein the first lancet (21) is mounted inside the pusher (233); and/or the presence of a gas in the gas,
the plate body (22) is provided with a second catch (221), the second catch (221) being configured to catch the chip body (3).
11. Microfluidic chip assembly according to claim 10, wherein the chip body (3) comprises:
a connecting part (32) provided with a third buckle (321) and an inner concave part (322), wherein the third buckle (321) is connected with the second buckle (221) in a clamping way, and the first buckle (232) is inserted into the inner concave part (322); and
the microfluidic part (33) is provided with a microfluidic channel (331), and the second puncture needle (31) is communicated with the microfluidic channel (331).
12. Microfluidic chip assembly according to claim 1, wherein at least one of the first (21) and second (31) lancet is arranged in a row.
13. Microfluidic chip assembly according to claim 1, wherein at least one of the first seal (12) and the second seal (13) comprises a sealing film.
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CN108489904A (en) * 2018-04-28 2018-09-04 东莞德益生物医疗科技有限公司 A kind of reaction vessel for detection
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CN111187714A (en) * 2020-02-21 2020-05-22 厦门大学 Micro-fluidic chip assembly and micro-fluidic chip detection system
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CN102454794A (en) * 2010-10-20 2012-05-16 株式会社爱德克斯 Liquid seal, shaft seal apparatus and pump apparatus using the same
CN108489904A (en) * 2018-04-28 2018-09-04 东莞德益生物医疗科技有限公司 A kind of reaction vessel for detection
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