CN115212932A - Microfluidic chip components - Google Patents

Abstract

The invention discloses a microfluidic chip assembly, relates to the technical field of biological assay or inspection, and is used for optimizing the performance of the microfluidic chip assembly. The microfluidic chip assembly comprises a reagent bin, a reagent bin bracket and a chip body. The reagent cabin comprises a cabin body, a first sealing element and a second sealing element; the storehouse body has the storage chamber, and the one end of the storehouse body is provided with first sealing member, and the other end of the storehouse body is provided with the second sealing member more than two-layer. The reagent cartridge support has a first puncture needle. The chip body is provided with a second puncture needle; the chip body can be dismantled with reagent storehouse support and be connected, and chip body and reagent storehouse support form and place the region to hold the reagent storehouse. Wherein the first lancet corresponds to the first seal and the second lancet corresponds to the second seal. The microfluidic chip assembly provided by the technical scheme has good sealing performance, reduces the possibility of substance leakage in the storage cavity of the reagent bin, and improves the use reliability of the microfluidic chip assembly.

Description

Microfluidic chip components

technical field

The invention relates to the technical field of biological assay or inspection, in particular to a microfluidic chip assembly.

Background technique

Nucleic acid testing is currently one of the gold standards for infectious disease diagnosis. The traditional nucleic acid detection methods are complicated in steps, and have high requirements on the testing site and the qualifications of operators. Microfluidic chip, also known as lab-on-a-chip, can realize highly integrated and integrated POCT (point-of-care test) based on microfluidic chip technology, greatly shorten the time required for detection, and realize on-demand inspection.

The microfluidic chip has multiple micro-sized flow channels and cavities, and these flow channels are arranged in a certain rule. During the test process, different biological reagents are released in a certain order, and flow to the designated cavity through different flow channels to complete the test. Various biochemical reactions, and finally realize the rapid and accurate detection of nucleic acid.

SUMMARY OF THE INVENTION

An embodiment of the present invention provides a microfluidic chip assembly, including:

The reagent chamber includes a chamber body, a first sealing member and a second sealing member; the chamber body has a storage cavity, one end of the chamber body is provided with the first sealing member, and the other end of the chamber body is provided with two the second seal above the layer;

a reagent cartridge holder having a first puncture needle; and

The chip body is provided with a second puncturing needle; the chip body is detachably connected to the reagent chamber support, and the chip body and the reagent chamber support form a placement area to accommodate the reagent chamber;

Wherein, the first piercing pair corresponds to the first sealing member, and the second piercing pair corresponds to the second sealing member.

In some embodiments, along the axial direction of the reagent cartridge, the storage cavity is divided into a storage section, a narrow section and a sealing section; the inner diameter of the narrow section is smaller than the storage section and the sealing section respectively inner diameter size.

In some embodiments, the second sealing members are located in the sealing section, and each of the second sealing members is distributed along the axial direction of the reagent cartridge.

In some embodiments, the sealing section includes a transition section and an installation section; the inner diameter of the transition section is smaller than the inner diameter of the installation section; the transition section is connected to the narrow section.

In some embodiments, the microfluidic chip assembly further includes:

The sealing ring is arranged on the installation section of the sealing section, and the sealing ring is configured to abut and seal against the outer wall of the second puncturing needle that has pierced the second sealing member.

In some embodiments, at least one of the second seals is located upstream of the seal ring and the remainder of the second seals are located downstream of the seal ring.

In some embodiments, the microfluidic chip assembly further includes:

A partition is arranged inside the storage section to divide the storage section into a plurality of regions; the partition is provided with a through hole to connect each of the regions.

In some embodiments, the inner wall of the storage segment is provided with a mounting portion that is convex or concave toward the interior of the storage segment, and the partition is mounted to the mounting portion.

In some embodiments, the reagent cartridge holder includes:

a plate body, which is detachably connected to the chip body; and

a top cover, which is rotatably arranged on one end of the plate body and is detachably connected to the chip body;

Wherein, the top cover is located on the outer side of the first sealing member of the reagent chamber, and the second puncturing needle is located on the outer side of the second sealing member; the plate body, the top cover and the chip body Co-encapsulate the reagent compartment.

In some embodiments, the top cover includes a cover body, a first buckle and a pusher; one side of the cover body is rotatably connected to the plate body; the first buckle is disposed away from the top cover one side of the plate body is configured to buckle the chip body; the pusher is configured in an annular shape and is mounted on the top cover; wherein, the first puncturing needle is mounted on the pusher internal; and/or,

The plate body is provided with a second buckle, and the second buckle is configured to buckle the chip body.

In some embodiments, the chip body includes:

a connection part, which is provided with a third buckle and an inner recess, the third buckle is connected with the second buckle, and the first buckle is inserted into the inner recess; and

The microfluidic part is provided with a microfluidic channel, and the second puncturing needle is communicated with the microfluidic channel.

In some embodiments, at least one of the first lancing needle and the second lancing needle is arranged in a row.

In some embodiments, at least one of the first seal and the second seal includes a sealing membrane.

The microfluidic chip assembly provided by the above technical solution is provided with more than two layers of second sealing members at the other end of the reagent chamber. After the second puncturing needle pierces the second sealing member, since the second sealing member has two layers, it can play a double-layer sealing effect, reduce the possibility of material leakage in the storage cavity of the reagent chamber, and improve the microfluidic chip. reliability of components.

Description of drawings

The accompanying drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the attached image:

1 is a schematic diagram of an exploded structure of a microfluidic chip assembly provided by an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a reagent compartment of a microfluidic chip assembly provided by an embodiment of the present invention;

Figure 3a is a schematic structural diagram of a reagent chamber of a microfluidic chip assembly provided by other embodiments of the present invention;

3b is a schematic structural diagram of a separator of a microfluidic chip assembly provided by other embodiments of the present invention;

FIG. 4 is a schematic structural diagram of a reagent cartridge bracket of a microfluidic chip assembly provided by an embodiment of the present invention;

5 is a schematic diagram of a partial three-dimensional structure of a chip body of a microfluidic chip assembly provided by an embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a front view of a chip body of a microfluidic chip assembly provided by an embodiment of the present invention.

Detailed ways

The technical solutions provided by the present invention will be described in more detail below with reference to FIGS. 1 to 6 .

Referring to FIG. 1 , an embodiment of the present invention provides a microfluidic chip assembly, including a reagent chamber 1 , a reagent chamber support 2 and a chip body 3 . Referring to FIG. 2 , the reagent cartridge 1 includes a cartridge body 11 , a first sealing member 12 and a second sealing member 13 . The bin body 11 has a storage cavity 10 , one end of the bin body 11 is provided with a first sealing member 12 , and the other end of the bin body 11 is provided with two or more layers of second sealing members 13 . Referring to FIG. 4 , the reagent cartridge holder 2 has a first puncturing needle 21 . 1 , 5 and 6 , the chip body 3 has a second puncturing needle 31 ; the chip body 3 is detachably connected to the reagent chamber support 2 , and the chip body 3 and the reagent chamber support 2 form a placement area to accommodate the reagent chamber 1. The first puncturing needle 21 corresponds to the first sealing member 12 , and the second puncturing needle 31 corresponds to the second sealing member 13 .

Referring to FIG. 1 , the reagent compartment bracket 2 cooperates with the chip body 3 to provide support and fixation for the reagent compartment 1, and to provide constraints for the reagent compartment 1 to move in the vertical direction shown in FIG. The reagent chamber 1 can be effectively fixed in both the unused state and the used state of the microfluidic chip assembly. The unused state refers to the state of the first seal 12 and the second seal 13 before they are punctured; the used state refers to the state of using the microfluidic chip assembly for testing, that is, the first seal 12 and the second seal 13 to puncture to release the reagent in the storage chamber 10 of the reagent cartridge 1.

The first puncturing needle 21 corresponds to the first sealing member 12, which means that after each component of the microfluidic chip assembly is installed in place, the first puncturing needle 21 is located near the first sealing member 12, and the direction shown in FIG. 1 is For example, the first puncturing needle 21 is located above the first sealing member 12; when subsequent use is required, by changing the distance between the first puncturing needle 21 and the first sealing member 12, the first puncturing needle 21 will A seal 12 is pierced.

The second puncturing needle 31 corresponds to the second sealing member 13 , which means that after each component of the microfluidic chip assembly is installed in place, the second puncturing needle 31 is located near the second sealing member 13 , and the direction shown in FIG. 1 is For example, the second puncturing needle 31 is located below the second sealing member 13 . When subsequent use is required, the second puncturing needle 31 can pierce the second sealing member 13 by changing the distance between the second puncturing needle 31 and the second sealing member 13 .

The storage chamber 10 of the reagent chamber 1 is used for storing liquid reagents or lyophilized reagents. The data of the reagent chamber 1 is determined according to the number of the second puncturing needles 31 of the chip body 3 , and can be flexibly increased or decreased according to the flow requirements of the biochemical reaction. The reagent chambers 1 are arranged in rows, and each reagent chamber 1 is correspondingly provided with a first puncturing needle 21 and a second puncturing needle 31 . All the reagent chambers 1 are fixed together to form the whole reagent chamber.

The first sealing member 12 and each second sealing member 13 of the reagent chamber 1 realize the whole process of sealing in the process of releasing the reagent in the storage chamber 10 . Each second sealing member 13 is installed by heat-press sealing, and the first sealing member 12 is heat-pressed and sealed after the reagent is loaded.

Referring to FIG. 2 , in some embodiments, the storage cavity 10 is divided into a storage section 101 , a narrow section 102 and a sealing section 103 along the axial direction of the reagent container 1 , that is, the length direction of the reagent container 1 . The narrow section 102 is located between the storage section 101 and the sealing section 103 . The inner diameter of the narrow section 102 is smaller than the respective inner diameters of the storage section 101 and the sealing section 103 .

The inner diameter of the storage section 101 is configured to be gradual, the inner diameter of the side of the storage section 101 facing the narrow section 102 is small, and the inner diameter of the storage section 101 away from the narrow section 102 is large, so as to facilitate subsequent release of the reagent.

The inner diameter of the narrow section 102 is relatively small, and when necessary, the inner diameter of the narrow section 102 can be set relatively small, so that the narrow section 102 and the outer wall of the second puncturing needle 31 that has pierced the second seal 13 There are small gaps in between.

Referring to FIG. 2 , in some embodiments, the second sealing members 13 are located in the sealing section 103 , and each second sealing member 13 is distributed along the axial direction of the reagent cartridge 1 . For example, the second sealing members 13 adopt a sheet-like structure, and each of the second sealing members 13 is installed separately to jointly play the role of multiple sealing.

Continuing to refer to FIG. 2, in some embodiments, the sealing section 103 includes a transition section 103a and a mounting section 103b. The inner diameter of the transition section 103a is smaller than the inner diameter of the mounting section 103b. The transition section 103a is located between the narrow section 102 and the mounting section 103b. The transition section 103a is connected to the narrow section 102 . The inner diameter of the transition section 103 a and the installation section 103 b are both larger than the inner diameter of the narrow section 102 .

Continuing to refer to FIG. 2 , in some embodiments, the microfluidic chip assembly further includes a sealing ring 4 , the sealing ring 4 is disposed on the mounting section 103 b of the sealing section 103 , and the sealing ring 4 is configured to pierce the second sealing member 13 . The outer wall of the second puncturing needle 31 abuts against the seal. The sealing ring 4 is made of rubber, for example.

Microfluidic chip assembly with sealing ring 4, especially suitable for liquid reagents. During transportation, or when the first sealing member 12 and the second sealing member 13 at the end of the reagent container 1 are not pierced, the first sealing member 12 at the top of the reagent container 1 and the multi-layer sealing member 13 at the bottom of the reagent container The second sealing member 13 can effectively seal the liquid reagent.

In the process of using the microfluidic chip assembly, a downward force is applied to the reagent cartridge holder 2 , that is, a force in the direction of the second puncturing needle 31 , and the second puncturing needle 31 of the chip body 3 is used to puncture the reagent cartridge 1 The second sealing member 13 at the bottom, at this time, the outer wall of the second puncturing needle 31 will be tightly wrapped by the sealing ring 4 to prevent leakage; The first piercing needle 21 pierces the first sealing member 12 on the top of the reagent container 1, so that the storage cavity 10 of the reagent container 1 is communicated with the external atmosphere, so as to realize the release of the reagent. At this time, due to the sealed connection between the sealing ring 4 and the second puncturing needle 31 of the chip body 3, the liquid in the storage cavity 10 will not leak.

Referring to FIG. 2 , in some embodiments, at least one of the second seals 13 is located upstream of the seal ring 4 , and the remaining second seals 13 are located downstream of the seal ring 4 . At least one of the plurality of second seals 13 is located upstream of the seal ring 4 , and the remaining second seals 13 are located downstream of the seal ring 4 .

After the sealing ring 4 is installed, due to the sealing effect of the second sealing member 13 located upstream of the sealing ring 4, the reagent chamber 1 prevents the sealing ring 4 from soaking in the reagent during the storage process, thereby reducing the life of the sealing ring 4, and avoiding the The reagent reacts with the sealing ring 4 to make the reagent deteriorate and fail.

It can be seen that the above technical solution not only improves the sealing performance of the microfluidic chip assembly in an unused state, but also improves the sealing performance of the microfluidic chip assembly in a used state.

Referring to FIGS. 3a and 3b, in other embodiments, the microfluidic chip assembly further includes a spacer 5, and the spacer 5 is disposed inside the storage segment 101 to divide the storage segment 101 into a plurality of regions, each region being Constructed to place a biological material. The spacer 5 is provided with through holes 50 therethrough to communicate the various regions.

If it is suitable for lyophilized reagents, the microfluidic chip assembly may not be provided with the sealing ring 4 . At this time, the separator 5 is used to divide the storage section 101 into a plurality of sections, as shown in FIG. 3 a and FIG. 3 b , and different lyophilized reagents can be placed in each section. At this time, the second sealing member 13 at the bottom of the reagent chamber 1 also plays the role of double-layer sealing, so as to improve the sealing performance of the microfluidic chip assembly and facilitate the realization of rapid nucleic acid on-site detection.

When the reagent lyophilized spheres are loaded, the section located downstream of the partition 5 accommodates the lyophilized spheres, and the section located upstream of the partition 5 accommodates the desiccant, so as to prolong the storage time of the lyophilized spheres.

Referring to FIGS. 3 a and 3 b , in some embodiments, the inner wall of the storage segment 101 is provided with a mounting portion 104 that is convex or concave toward the interior of the storage segment 101 , and the partition 5 is mounted to the mounting portion 104 . The mounting portion 104 may be annular, or only convex or concave in a partial area of the inner wall of the storage section 101 in the circumferential direction. The mounting portion 104 is provided to facilitate the mounting of the spacer 5 .

The specific implementation of the reagent cartridge holder 2 is described below.

Referring to FIGS. 4 and 5 , in some embodiments, the reagent cartridge holder 2 includes a plate body 22 and a top cover 23 . The plate body 22 is detachably connected to the chip body 3 . The top cover 23 is rotatably disposed on one end of the plate body 22 , and the top cover 23 is detachably connected to the chip body 3 . The top cover 23 is located outside the first sealing member 12 of the reagent chamber 1 , and the second puncturing needle 31 is located outside the second sealing member 13 . The plate body 22 , the top cover 23 and the chip body 3 enclose the reagent chamber 1 together.

The top cover 23 is mounted on the plate body 22 in a movable and switchable position. The connection point M between the top cover 23 and the plate body 22 can be made of deformable materials, such as rubber, elastic parts, etc.; Before the reagent chamber 1 is not installed, the top cover 23 is in the state shown in FIG. 4 relative to the plate body 22 . After the reagent chamber 1 is installed in place, the top cover 23 is rotated about 90°, and the top cover 23 and the chip body 3 are snapped together.

Referring to FIG. 4 , in some embodiments, the top cover 23 includes a cover body 231 , a first buckle 232 and a pusher 233 . One side of the cover body 231 is rotatably connected with the plate body 22 . The first buckle 232 is disposed on the side of the top cover 23 away from the plate body 22 and is configured to buckle the chip body 3 . The pusher 233 is configured in an annular shape, and the pusher 233 is mounted on the top cover 23 . The first puncturing needle 21 is installed inside the pusher 233 .

The cover body 231 is configured as a rectangular open cover-like structure, and the cover body 231 serves as an outer frame of the top cover 23 . A pusher 233 is provided inside the outer frame. Specifically, the pusher 233 and the top cover 23 can be connected together by connecting ribs. The propeller 233 is generally a frame-like structure or a semi-enclosed structure surrounded by a plurality of plates. In the embodiment shown in FIG. 4, the pusher 233 is a rectangular annular wall. Inside the area enclosed by the pusher 233, the second puncturing needle 31 is provided. The second puncturing needle 31 can also be connected to the pusher 233 through a rib. The number of the second puncturing needles 31 matches the number of the microfluidic channels 331 on the plate body 22 . The first snap 232 is a protrusion disposed on the edge of the cover body 231 , and is snapped into the concave portion 322 of the chip body 3 to realize detachable connection between the top cover 23 and the chip body 3 .

Referring to FIG. 4 , the board body 22 is provided with a second buckle 221 , and the second buckle 221 is configured to buckle the chip body 3 . The second buckles 221 buckle both sides of the edge of the chip body 3 to realize the detachable connection between the board body 22 and the chip body 3 .

Referring to FIG. 4 , in order to improve the piercing effect of the first puncturing needle 21 , in some embodiments, the outer wall of the puncturing needle is configured with a pointed end, and the first sealing member 12 can be pierced by the pointed end. Specifically, in the cross-sectional direction: the outer wall of the first puncturing needle 21 is configured in a quadrangular, triangular, polygonal, or other irregular shape.

The specific implementation manner of the chip body 3 is described below.

Referring to FIGS. 5 and 6 , in some embodiments, the chip body 3 includes a connection part 32 and a microfluidic part 33 . The connecting portion 32 is provided with a third buckle 321 and an inner concave portion 322 , the third buckle 321 is connected with the second buckle 221 by engaging, and the first buckle 232 is inserted into the inner concave portion 322 . The microfluidic part 33 is provided with a microfluidic channel 331 , and the second puncturing needle 31 communicates with the microfluidic channel 331 . The arrangement of the microfluidic channels 331 can be set as required. The second puncturing needle 31 is hollow, and the second puncturing needle 31 is located upstream of the microfluidic channel 331 , and the reagent flows from the reagent cartridge 1 through the second puncturing needle 31 to the microfluidic channel 331 .

Referring to FIGS. 1 and 5 , in some embodiments, at least one of the first puncturing needles 21 and the second puncturing needles 31 is arranged in a row. The first puncturing needle 21 and the second puncturing needle 31 may adopt hollow needle bodies, that is, the wall body of the needle body plays a puncturing function, and the inner area of the needle body is hollow to play the role of circulating fluid.

The manufacturing and use process of the microfluidic chip assembly is described below, taking the microfluidic chip assembly with the sealing ring 4 as an example, that is, the microfluidic chip assembly is suitable for liquid reagents as an example.

Referring to FIG. 2, first, the reagent cartridge 1 is prepared. First, the second sealing member 13 located at the most downstream is fixed by hot pressing, and the bottom of the reagent chamber 1 is preliminarily sealed. Next, the sealing ring 4 is installed again; then, the second sealing member 13 located upstream of the sealing ring 4 is fixed by hot pressing. Taking the second sealing member 13 as two layers as an example, a structure in which the sealing ring 4 is located in the middle and the two second sealing members 13 are located on both sides is finally formed, that is, the second sealing member 13 - the sealing ring 4 - the second sealing member 13 Structure.

Secondly, according to the different needs of the biochemical reaction, select the corresponding reagents. Liquid reagents can be injected directly into the chamber. The chamber reserved for the sample can also be equipped with a filter device in advance as required to help purify the sample to be tested. After the reagents are filled, the first sealing member 12 is fixed by means of thermal pressure sealing, so as to realize the complete sealing of the reagent chamber 1, so as to facilitate the storage and transportation of the microfluidic chip assembly. It should be additionally explained that, if it is a solid reagent, the immobilization reagent is placed downstream of the storage section 101 first, and then the desiccant is filled in the upstream section of the partition 5 to keep the chamber dry for a long time and prolong the storage time of the solid reagent.

When testing is required, first, the operator installs the reagent cartridge holder 2 , and fastens the plate body 22 of the reagent cartridge holder 2 with the chip body 3 to form a space that can hold the reagent cartridge 1 .

Then, select the corresponding reagent chamber 1 according to the detection requirements, and after the extracted sample is injected into the sample chamber, the reagent chamber 1 is placed between the plate body 22 and the chip body 3 in the correct orientation shown in FIG. 1 , and then the reagent chamber is fastened. The top cover 23 of the bracket 2 . In this embodiment, for nucleic acid detection, a reagent chamber 1 with eleven storage chambers 10 is selected, wherein ten storage chambers 10 are pre-packaged with detection reagents, and one sample chamber is reserved for loading samples to be tested.

Then, put the preliminarily assembled chip into the testing apparatus, and start the testing apparatus. The detection instrument outputs a certain pressure, and firstly, the top cover 23 is pressed down, and the pusher 233 is pressed away from the cover body 231 of the top cover 23 . As the downward pressure increases, the pusher 233 will press the reagent cartridge 1 to increase the contact force between the reagent cartridge 1 and the second puncturing needle 31 , thereby piercing the double-layer film in the lower sealing area of the reagent cartridge 1 until the second puncturing needle 31 An interference fit with the sealing ring 4 ensures no leakage.

After the interference fit between the reagent chamber 1 and the second puncture needle 31 of the chip body 3 is completed, the stroke of the pressure output surface of the instrument increases again, squeezes the inside of the pusher 233, and presses the first puncture needle 21 away from the pusher 233, puncturing the pusher 233. The first sealing member 12 of the reagent chamber 1 is broken to realize the communication with the atmosphere. After the connection, the reagents can be released normally, and the reagents can be taken in sequence by controlling the instrument according to the needs of the reaction. The whole process needs to maintain the down pressure and maintain the interference fit to prevent leakage and pollution.

Finally, when the test is over, the pressing force can be removed, the chip can be taken out, and the next chip can be assembled for a new set of tests.

The microfluidic chip assembly provided by the above technical solution is easy to operate and has low requirements for operators. It only needs to install the reagent chamber bracket 2, and then put the reagent chamber 1 preloaded with the corresponding biochemical reaction reagents into it, and close the top cover. 23 is placed into the relative instrument, and the instrument outputs a certain pressure to make the reagent chamber 1 communicate with the chip body 3, which is ready for the next detection work.

In the description of the present invention, it should be understood that the terms "center", "portrait", "horizontal", "front", "rear", "left", "right", "vertical", "horizontal", The orientation or positional relationship indicated by "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or obscuring It means that the referred device or element must have a specific orientation, be constructed and operate for a specific orientation, and therefore cannot be understood as a limitation on the protection content of the present invention.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that it can still be Modifications are made to the technical solutions described in the foregoing embodiments, or some technical features thereof are equivalently replaced, but these modifications or replacements do not make the essence of the corresponding technical solutions depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (13)

1. A microfluidic chip assembly, comprising:

the reagent cabin (1) comprises a cabin body (11), a first sealing element (12) and a second sealing element (13); the bin body (11) is provided with a storage cavity (10), one end of the bin body (11) is provided with the first sealing element (12), and the other end of the bin body (11) is provided with more than two layers of the second sealing elements (13);

a reagent cartridge holder (2) having a first piercing needle (21); and

a chip body (3) having a second puncture needle (31); the chip body (3) is detachably connected with the reagent bin support (2), and the chip body (3) and the reagent bin support (2) form a placing area for accommodating the reagent bin (1);

wherein the first lancet (21) corresponds to the first seal (12) and the second lancet (31) corresponds to the second seal (13).

2. Microfluidic chip assembly according to claim 1, wherein the storage chamber (10) is divided into a storage section (101), a narrow section (102) and a sealing section (103) along the axial direction of the reagent cartridge (1); the narrow section (102) has an inner diameter dimension that is less than the respective inner diameter dimensions of the storage section (101) and the sealing section (103).

3. Microfluidic chip assembly according to claim 2, wherein the second seals (13) are located at the sealing section (103) and each of the second seals (13) is arranged dispersed along the axial direction of the reagent cartridge (1).

4. The microfluidic chip assembly according to claim 3, wherein the sealing section (103) comprises a transition section (103 a) and a mounting section (103 b); the inner diameter of the transition section (103 a) is smaller than that of the mounting section (103 b); the transition section (103 a) is connected to the throat section (102).

5. The microfluidic chip assembly of claim 2, further comprising:

a sealing ring (4) disposed at a mounting section (103 b) of the sealing section (103), the sealing ring (4) being configured to seal against an outer wall of a second lancet (31) that punctures the second sealing member (13).

6. Microfluidic chip assembly according to claim 5, wherein at least one of the second seals (13) is located upstream of the sealing ring (4) and the remaining second seals (13) are located downstream of the sealing ring (4).

7. The microfluidic chip assembly of claim 2, further comprising:

a partition (5) provided inside the storage section (101) to divide the storage section (101) into a plurality of regions; the partition (5) is provided with a through hole (50) therethrough to communicate the respective regions.

8. Microfluidic chip assembly according to claim 7, wherein the inner wall of the storage section (101) is provided with a mounting portion (104) that is convex or concave towards the inside of the storage section (101), the partition (5) being mounted to the mounting portion (104).

9. Microfluidic chip assembly according to claim 1, wherein the reagent cartridge holder (2) comprises:

the plate body (22) is detachably connected with the chip body (3); and

the top cover (23) is rotatably arranged at one end of the plate body (22) and is detachably connected with the chip body (3);

wherein the top cover (23) is positioned outside the first seal (12) of the reagent cartridge (1), and the second lancet (31) is positioned outside the second seal (13); the plate body (22), the top cover (23) and the chip body (3) jointly wrap the reagent cabin (1).

10. The microfluidic chip assembly according to claim 9, wherein the top cover (23) comprises a cover (231), a first snap (232), and a pusher (233); one side of the cover body (231) is rotatably connected with the plate body (22); the first buckle (232) is arranged on one side of the top cover (23) far away from the plate body (22) and is configured to buckle the chip body (3); the pusher (233) is configured to be annular, mounted to the top cover (23); wherein the first lancet (21) is mounted inside the pusher (233); and/or the presence of a gas in the gas,

the plate body (22) is provided with a second catch (221), the second catch (221) being configured to catch the chip body (3).

11. Microfluidic chip assembly according to claim 10, wherein the chip body (3) comprises:

a connecting part (32) provided with a third buckle (321) and an inner concave part (322), wherein the third buckle (321) is connected with the second buckle (221) in a clamping way, and the first buckle (232) is inserted into the inner concave part (322); and

the microfluidic part (33) is provided with a microfluidic channel (331), and the second puncture needle (31) is communicated with the microfluidic channel (331).

12. Microfluidic chip assembly according to claim 1, wherein at least one of the first (21) and second (31) lancet is arranged in a row.

13. Microfluidic chip assembly according to claim 1, wherein at least one of the first seal (12) and the second seal (13) comprises a sealing film.

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