CN115181677A - Preparation method of composite biomass material for treating phenolic wastewater - Google Patents
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Abstract
The invention relates to the technical field of biological treatment of sewage and wastewater, and provides a preparation method of a composite biomass material for treating phenolic wastewater, which comprises the following steps: s1, inoculating Aspergillus niger (Aspergillus niger) spore suspension into a mycelium pellet preparation culture medium for culture; s2, inoculating the phenolic degrading bacteria (Alcaligenes sp.) suspension and tourmaline into a culture medium for continuous culture to obtain the composite biomass material. Through the technical scheme, the prepared composite biomass material has good phenolic wastewater treatment efficiency, and simultaneously solves the problems of functional flora loss and delayed start of a treatment system in the traditional treatment method.
Description
Technical Field
The invention relates to the technical field of biological treatment of sewage and wastewater, in particular to a preparation method of a composite biomass material for treating phenolic wastewater.
Background
The phenol-containing wastewater is widely produced in industries such as phenolic resin, medicines, paints, lubricants and the like, and because phenolic compounds have high toxicity under low concentration, the phenol-containing wastewater is extremely harmful to the safety of human beings and the natural environment when discharged outside, the development of an effective phenol-containing wastewater treatment technology is particularly critical.
The biological method has the remarkable advantages of large treatment capacity, low cost, wide application range, no secondary pollution and the like in the aspect of degrading the phenolic compounds in the water. However, the problems of effective microorganism loss, long system starting time, strain degradation, low degradation efficiency, sludge poisoning and the like in the wastewater treatment process still influence the application and popularization of the biological method for treating the phenolic wastewater.
Disclosure of Invention
The invention provides a preparation method of a composite biomass material for treating phenolic wastewater, which is used for preparing the composite biomass material for treating the phenolic wastewater, has stable structural performance and is easy to recycle, and solves the problems of effective microorganism loss, delayed starting of a treatment system and low initial degradation efficiency in the process of degrading the phenolic wastewater by a biological method in the prior art.
The technical scheme of the invention is as follows:
the preparation method of the composite biomass material for treating the phenolic wastewater comprises the following steps:
s1, inoculating Aspergillus niger (Aspergillus niger) spore suspension into a mycelium pellet preparation culture medium for culture;
s2, inoculating the phenolic degrading bacteria (Alcaligenes sp.) suspension and tourmaline into a culture medium for continuous culture to obtain the composite biomass material.
The tourmaline is nano powder tourmaline with the grain diameter of 1500-2500 meshes as a further technical proposal;
preferably, the tourmaline is soaked and disinfected in 75% ethanol for 15-20min, and washed with deionized water for 3-5 times;
preferably, the tourmaline accounts for 0.01-0.02% of the mass of the mycelium pellet preparation culture medium.
As a further technical scheme, in the step S1, the concentration of the Aspergillus niger spore suspension is 10 7 -10 8 Per mL;
preferably, the aspergillus niger spore suspension is inoculated into a mycelium pellet preparation culture medium according to the volume ratio of 0.5-1% for culture.
As a further technical scheme, in the step S1, the mycelium pellet preparation medium comprises the following components: sucrose 10g/L, NH 4 CI 1g/L，K 2 HPO 4 ·3H 2 O 0.5g/L，KH 2 PO 4 0.5g/L，MgSO 4 0.5g/L and 1000mL of deionized water.
As a further technical scheme, in the step S1, the culture time is 24-48h; preferably, the culture temperature is 30-35 ℃, and the constant temperature shaking culture is 140-160 r/min.
As a further technical scheme, in the step S2, the concentration of the bacterial suspension of the phenol degrading bacteria is 10 8 -10 10 one/mL.
As a further technical scheme, the phenolic degrading bacteria suspension accounts for 3-5% of the volume of the mycelium pellet preparation culture medium.
As a further technical scheme, in the step S2, the cultivation is continued for 24-48h; preferably, the culture temperature is 30-35 ℃, and the constant temperature shaking culture is carried out at 140-160 r/min.
As a further technical scheme, the oscillation speed is 160r/min at the initial stage of mycelium pellet culture, and is properly reduced with the increase of culture time, but is not lower than 140r/min.
As a further technical scheme, after the composite biomass material is prepared, the culture medium is filtered, and the trapped composite biomass material is washed by deionized water to remove the residual culture medium.
According to the composite biomass material obtained by the preparation method of the composite biomass material for treating the phenolic wastewater, 2-10cm microsphere particles of phenolic degrading bacteria and tourmaline are loaded by taking Aspergillus niger mycelium pellets as a biomass carrier.
The beneficial effects of the invention are as follows:
1. the invention finds that the composite biomass material prepared by taking the fungal mycelium pellets as the main material and combining the phenolic degradation functional flora and the tourmaline can solve the problems of effective microorganism loss, delayed starting of a treatment system and low initial degradation efficiency to a certain extent when treating phenolic wastewater, and meanwhile, the composite biomass material is beneficial to recovery and can be repeatedly used.
2. The growth condition of mycelium pellets formed by the aspergillus niger is easy to control, the sedimentation performance in an aqueous solution is good, the adsorption capacity is strong, phenols are well tolerated, the mycelium pellets can be tightly combined with a phenol degradation functional flora, and the composite biomass material has advantages when being used as a biomass carrier of the composite biomass material. The mycelium pellet is formed by interweaving and winding Aspergillus niger mycelium, has large specific surface area and high porosity, and is beneficial to the attachment and mass transfer of bacteria. Extracellular polymers secreted on the surface of hyphae take polysaccharide as a main component, have certain viscosity, are beneficial to bacteria attachment, also have certain protection on the bacteria and improve the shock load resistance of flora.
3. Tourmaline has far infrared radiation effect and spontaneous electric polarity, and can reduce water molecule cluster volume, enhance solute permeability in solution, and promote growth and reproduction of microorganism. Tourmaline is added when the composite biomass material of the phenol wastewater is prepared, so that the environmental adaptability of the composite material is greatly improved, and the start-up of a water treatment system is accelerated. However, when tourmaline is used alone for wastewater treatment, the effect of degrading phenolic compounds is poor.
4. When the activated sludge reinforced phenolic degradation flora is only adopted to treat phenolic wastewater, the effluent reaching stabilization time is longer, and the lag period is overlong, but in the invention, aspergillus niger mycelium pellets are taken as a biomass carrier, and the phenolic degradation bacteria and tourmaline are loaded, so that the synergistic effect is realized, the degradation capability of phenolic compounds is improved, the start-up of a sewage treatment system is greatly accelerated, and the effluent is stable.
Drawings
The invention is described in further detail below with reference to the drawings and the detailed description.
FIG. 1 is a photograph of a mycelial pellet obtained in step S1 in example 5;
FIG. 2 is a photograph of the composite biomass material of example 5;
FIG. 3 is an SEM picture of the mycelium pellet obtained in step S1 of example 5;
fig. 4 is an SEM picture of the composite biomass material of example 5.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall be included within the scope of protection of the present invention.
Example 1
A method for preparing a composite biomass material, comprising the steps of:
s1, adjusting the concentration to 10 7 Inoculating Aspergillus niger spore suspension into mycelium pellet preparation culture medium according to 0.5% volume ratio, and performing constant temperature shaking culture at 33 deg.C and 160r/min for 48h, wherein the mycelium pellet preparation culture medium comprises: sucrose 10g/L, NH 4 CI 1g/L，K 2 HPO 4 ·3H 2 O 0.5g/L，KH 2 PO 4 0.5g/L，MgSO 4 0.5g/L and 1000mL of deionized water.
S2, concentration of 10 10 The phenol degrading bacteria suspension per mL is inoculated into the culture medium according to the volume ratio of 4 percent and the tourmaline according to the mass ratio of 0.02 percent, and the mixture is subjected to constant temperature oscillation at the speed of 160r/min for continuous culture for 48 hours.
The 2-10cm microsphere particles which take aspergillus niger wire balls as biological carriers and carry phenol degrading bacteria and tourmaline are prepared.
Example 2
A preparation method of a composite biomass material comprises the following steps:
s1, adjusting the concentration to 10 7 Inoculating Aspergillus niger spore suspension into a mycelium pellet preparation culture medium according to the volume ratio of 0.4%, performing constant-temperature shaking culture at the temperature of 30 ℃ and the speed of 150r/min for 48h, wherein the components of the mycelium pellet preparation culture medium are as follows: sucrose 10g/L, NH 4 CI 1g/L，K 2 HPO 4 ·3H 2 O 0.5g/L，KH 2 PO 4 0.5g/L，MgSO 4 0.5g/L and 1000mL of deionized water.
S2, concentration of 10 10 The phenol degrading bacteria suspension per mL is inoculated into a culture medium according to the volume ratio of 3 percent and the tourmaline according to the mass ratio of 0.02 percent, and the culture is continued for 36 hours under the constant temperature oscillation of 150 r/min.
The 2-10cm microsphere particles which take aspergillus niger wire balls as biological carriers and carry phenol degrading bacteria and tourmaline are prepared.
Example 3
A method for preparing a composite biomass material, comprising the steps of:
s1, adjusting the concentration to 10 8 Inoculating Aspergillus niger spore suspension into a mycelium pellet preparation culture medium according to the volume ratio of 0.3% for 35 ℃, and carrying out constant-temperature shaking culture at 160r/min for 48h, wherein the components of the mycelium pellet preparation culture medium are as follows: sucrose 10g/L, NH 4 CI 1g/L，K 2 HPO 4 ·3H 2 O 0.5g/L，KH 2 PO 4 0.5g/L，MgSO 4 0.5g/L and 1000mL of deionized water.
S2, concentration of 10 9 The phenol degrading bacteria suspension is inoculated into a culture medium according to the volume ratio of 5 percent and the tourmaline according to the mass ratio of 0.01 percent, the constant temperature oscillation is carried out for continuous culture for 24 hours at 140r/min, wherein the tourmaline adopts nano powder tourmaline with the grain diameter of about 2000 meshes, the ethanol with the concentration of 75 percent is adopted for soaking and disinfection for 20min, and the deionized water is used for washing for 3 times.
The 2-10cm microsphere particles which take aspergillus niger wire balls as biological carriers and carry phenol degrading bacteria and tourmaline are prepared.
Example 4
A method for preparing a composite biomass material, comprising the steps of:
s1, adjusting the concentration to 10 8 Inoculating Aspergillus niger spore suspension of 0.3% volume ratioThe preparation culture medium of the mycelium pellet is subjected to constant temperature shaking culture at 34 ℃ and 160r/min for 48h, and the preparation culture medium of the mycelium pellet comprises the following components: sucrose 10g/L, NH 4 CI 1g/L，K 2 HPO 4 ·3H 2 O 0.5g/L，KH 2 PO 4 0.5g/L，MgSO 4 0.5g/L and 1000mL of deionized water.
S2, concentration of 10 9 The phenol degrading bacteria suspension is inoculated into a culture medium according to the volume ratio of 5 percent and the tourmaline according to the mass ratio of 0.01 percent, the constant temperature oscillation is carried out for continuous culture for 48 hours at 140r/min, wherein the tourmaline adopts nano powder tourmaline with the grain diameter of about 2000 meshes, the ethanol with the concentration of 75 percent is adopted for soaking and disinfection for 20min, and the deionized water is used for washing for 3 times.
And S3, after the composite biomass material is prepared, filtering the culture medium, washing the trapped composite biomass material with deionized water, and removing the residual culture medium.
The 2-10cm microsphere particles which take aspergillus niger wire balls as biological carriers and carry phenol degrading bacteria and tourmaline are prepared.
Example 5
A preparation method of a composite biomass material comprises the following steps:
s1, adjusting the concentration to 10 8 Inoculating Aspergillus niger spore suspension of each mL into a mycelium pellet preparation culture medium according to the volume ratio of 0.3%, performing constant-temperature shaking culture at 34 ℃ at 160r/min for 48h, wherein the mycelium pellet preparation culture medium comprises the following components: sucrose 10g/L, NH 4 CI 1g/L，K 2 HPO 4 ·3H 2 O 0.5g/L，KH 2 PO 4 0.5g/L，MgSO 4 0.5g/L and 1000mL of deionized water.
S2, concentration of 10 9 The phenol degrading bacteria suspension is inoculated into a culture medium according to the volume ratio of 5 percent and the tourmaline according to the mass ratio of 0.01 percent, the constant temperature oscillation is carried out for continuous culture for 48 hours at 140r/min, wherein the tourmaline adopts nano powder tourmaline with the grain diameter of about 2000 meshes, the ethanol with the concentration of 75 percent is adopted for soaking and disinfection for 20min, and the deionized water is used for washing for 3 times.
The 2-10cm microsphere particles which take aspergillus niger wire balls as biological carriers and carry phenol degrading bacteria and tourmaline are prepared.
The main components of the tourmaline used in the embodiment of the invention are shown in the table.
TABLE 1 tourmaline major ingredients and contents
In the embodiment of the invention, the Aspergillus niger spore suspension and the phenolic degrading bacteria suspension are prepared according to the following steps:
(1) A suspension of Aspergillus niger spores was prepared. In a sterile environment, selecting spores on a slope by using an inoculating loop, inoculating the spores into sterilized deionized water, counting the spores by adopting a blood counting method, and then diluting the spores to a specified concentration by using sterile deionized water.
(2) A suspension of phenol-degrading bacteria (Alcaligenes sp.) was prepared. In a sterile environment, strains on the inclined plane are picked by an inoculating loop, inoculated into sterilized deionized water, counted by adopting a blood counting method, and the bacterial suspension is adjusted to a specified concentration.
Wherein Aspergillus niger adopts Aspergillus niger Y3; PHC, and is separated from secondary sludge of a sewage treatment plant of a Jilin chemical plant, wherein the phenol degrading bacteria are Alcaligenes sp.PHC, and the separation method comprises the following steps:
preparing bacterial suspension, namely preparing secondary sludge of a Jilin chemical wastewater treatment plant into bacterial suspension;
enrichment culture-the enrichment culture medium selects a common culture medium added with phenol, the bacterial suspension is put into the enrichment culture medium, the mixture is put into a new enrichment culture medium for continuous culture after shaking culture is carried out for 48 hours at 30 ℃ and 120r/min, and the process is repeated for 5 times;
and (3) strain separation, namely performing gradient dilution on the bacterial suspension, taking 0.5mL of diluent with different concentrations to inoculate onto an inorganic salt agar plate culture medium added with phenol and ammonium nitrate, coating, performing constant-temperature culture for 48h at 30 ℃, selecting a single colony strain, and performing repeated streaking separation culture on the plate culture medium to obtain a pure strain, namely Alcaligenes sp.
FIG. 1 is a photograph of a mycelial pellet obtained in step S1 of example 5 of the present invention, and FIG. 3 is an SEM picture of a mycelial pellet in which a mycelial pellet is intertwined with Aspergillus niger hyphae, and has a large specific surface area and a high porosity.
Fig. 2 is a photograph of the composite biomass material of example 5, the composite biomass material having a nearly spherical structure, and fig. 4 is an SEM picture of the composite biomass material, in which tourmaline and phenolic degrading bacteria are attached to the mycelium pellet, the porosity is high, and the degrading and adsorbing ability to phenolic compounds is improved.
Experimental example: phenol-containing wastewater treatment experiment
No. 1 influent phenolic Compound concentration
2# SBR treatment system for reinforcing phenol degrading bacteria by adopting activated sludge and for treating effluent water with phenol compound concentration
Example 3# 5 composite Biomass Material SBR treatment System effluent phenolic Compound concentration
( Note: since the effects of embodiments 1-5 are similar, taking embodiment 5 as an example, the data of other embodiments are not repeated herein )
The other conditions of the SBR treatment systems 2# and 3# are the same, and the period is 12h, wherein the period comprises 5min water inlet, 600min aeration (2L/h), 20min sedimentation, hydraulic Retention Time (HRT) of 20h,5min water discharge and 90min idle time.
The effect of treating phenol-containing wastewater is shown in Table 2.
TABLE 2 Effect of composite biomass materials on treating phenol-containing wastewater
Note: 1# represents the influent phenolic compound concentration (mg/L); 2# represents the concentration (mg/L) of the phenolic compound in the effluent of the treatment system adopting the activated sludge reinforced phenolic degradation flora; and 3# represents the concentration (mg/L) of the phenolic compound in the effluent of the treatment system adopting the composite biomass material.
Wherein phenolic compounds in the wastewater mainly comprise phenol and cresol, the activated sludge strengthens a treatment system of phenolic degradation floras, and because the floras are lost and exist in a delay period, water is stable from the seventh period, and the phenolic compounds are not detected; however, in the treatment system of the composite biomass material obtained in the embodiment of the present invention, the effluent is stable from the second cycle, and no phenolic compound is detected. The composite biomass material prepared by the invention has better advantages in terms of the degradation effect of phenolic compounds and the starting speed of a system.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The preparation method of the composite biomass material for treating the phenolic wastewater is characterized by comprising the following steps of:
s1, inoculating Aspergillus niger (Aspergillus niger) spore suspension into a mycelium pellet preparation culture medium for culture;
s2, inoculating the phenolic degrading bacteria (Alcaligenes sp.) suspension and tourmaline into a culture medium for continuous culture to obtain the composite biomass material.
2. The method for preparing a composite biomass material according to claim 1, wherein the tourmaline is nano-powder tourmaline having a particle size of 1500-2500 mesh; preferably, the tourmaline is soaked and disinfected in 75% ethanol for 15-20min, and washed with deionized water for 3-5 times; preferably, the tourmaline accounts for 0.01-0.02% of the mass of the mycelium pellet preparation culture medium.
3. The method for preparing composite biomass material according to claim 1, wherein in the step S1, the concentration of Aspergillus niger spore suspension is 10 7 -10 8 Per mL; preferably, the Aspergillus niger spore suspension is inoculated into a mycelium pellet preparation culture medium according to the volume ratio of 0.5-1% for culture.
4. The method for preparing a composite biomass material according to claim 1, wherein in the step S1, the components of the mycelium pellet preparation medium are as follows: sucrose 10g/L, NH 4 CI 1g/L，K 2 HPO 4 ·3H 2 O 0.5g/L，KH 2 PO 4 0.5g/L，MgSO 4 0.5g/L and 1000mL of deionized water.
5. The method for preparing a composite biomass material according to claim 1, wherein in the step S1, the culture time is 24-48h; preferably, the culture temperature is 30-35 ℃, and the constant temperature shaking culture is carried out at 140-160 r/min.
6. The method for preparing a composite biomass material according to claim 1, wherein the concentration of the suspension of phenolic degrading bacteria in step S2 is 10 8 -10 10 one/mL.
7. The method for preparing a composite biomass material according to claim 1, wherein in the step S2, the cultivation is continued for 24-48h; preferably, the culture temperature is 30-35 ℃, and the constant temperature shaking culture is 140-160 r/min.
8. The method of preparing a composite biomass material according to claim 1, wherein said suspension of phenolic degrading bacteria is 3% to 5% by volume of said mycelial pellet preparation medium.
9. The method of claim 1, wherein after the composite biomass material is prepared, the culture medium is filtered off, and the entrapped composite biomass material is washed with deionized water to remove the residual culture medium.
10. The composite biomass material obtained by the preparation method of the composite biomass material for treating phenolic wastewater according to claim 1, wherein 2-10cm microsphere particles of phenolic degrading bacteria and tourmaline are loaded by taking aspergillus niger mycelium pellets as a biomass carrier.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4806482A (en) * | 1986-03-21 | 1989-02-21 | The B.F. Goodrich Company | Microbial degradation of hydrocarbons |
| CN102718327A (en) * | 2012-07-05 | 2012-10-10 | 浙江皇冠科技有限公司 | Nano-biological water body remediation agent for aquaculture and preparation method thereof |
| CN105505781A (en) * | 2014-10-20 | 2016-04-20 | 上海理工大学 | Method for controlling aspergillus filamentous fungi to form mycelium pellets |
-
2022
- 2022-07-13 CN CN202210825714.0A patent/CN115181677A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4806482A (en) * | 1986-03-21 | 1989-02-21 | The B.F. Goodrich Company | Microbial degradation of hydrocarbons |
| CN102718327A (en) * | 2012-07-05 | 2012-10-10 | 浙江皇冠科技有限公司 | Nano-biological water body remediation agent for aquaculture and preparation method thereof |
| CN105505781A (en) * | 2014-10-20 | 2016-04-20 | 上海理工大学 | Method for controlling aspergillus filamentous fungi to form mycelium pellets |
Non-Patent Citations (4)
| Title |
|---|
| SI ZHANG 等: "Biological improvement on combined mycelial pellet for aniline treatment by tourmaline in SBR process", vol. 102, no. 19, XP028276341, DOI: 10.1016/j.biortech.2011.06.075 * |
| 于莹: "生物制剂处理苯胺废水的效果研究", 中国优秀硕士学位论文全文数据库（电子期刊）工程科技I辑, pages 027 - 174 * |
| 张斯 等: "混合菌丝球苯胺净化效能研究", no. 06 * |
| 张斯: "菌丝球生物载体的构建及其强化废水处理效能研究", no. 2014, pages 027 - 61 * |
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