CN115177579A - Iron sulfide nano enzyme suppository and antibacterial application thereof - Google Patents
Iron sulfide nano enzyme suppository and antibacterial application thereof Download PDFInfo
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- CN115177579A CN115177579A CN202210072103.3A CN202210072103A CN115177579A CN 115177579 A CN115177579 A CN 115177579A CN 202210072103 A CN202210072103 A CN 202210072103A CN 115177579 A CN115177579 A CN 115177579A
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/02—Suppositories; Bougies; Bases therefor; Ovules
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
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- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
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- A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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Abstract
The invention provides an iron sulfide nano enzyme vaginal suppository, which comprises iron sulfide nano enzyme and B type gelatin. The iron sulfide nano enzyme suppository has the characteristics of simple prescription and high efficiency in establishing and maintaining vaginal flora balance, and can be widely applied to treating or preventing bacterial vaginitis, bacterial vaginosis and recurrent bacterial vaginosis.
Description
Technical Field
The invention relates to the field of pharmaceutical preparations, in particular to an iron sulfide nano enzyme suppository for treating or preventing bacterial vaginitis, bacterial vaginosis and recurrent bacterial vaginosis.
Background
Gelatin (Gelatin) is a peptide molecular polymer obtained by purifying collagen in animal skin, bone, tendon or ligament after appropriate hydrolysis, and is prepared into type A Gelatin by acid method and type B Gelatin by alkali method. Gelatin is widely used in the fields of food, rubber, cement, plastics, etc., and in the field of pharmaceutical preparations, gelatin is commonly used for preparing capsule shells. For the two types of gelatin, the prior art has not disclosed other differences in structure, properties, applications, etc., except that the isoelectric point of form a is slightly higher than that of form B.
Vaginitis (Vaginitis) is an inflammation that occurs in and under the vaginal mucosa and includes bacterial Vaginitis, mycotic Vaginitis, trichomonas Vaginitis, and mixed bacterial and fungal Vaginitis. Bacterial Vaginosis (BV) is a series of clinical vaginal dysbacteriosis caused by replacement of normal vaginal flora with Lactobacillus as dominant bacteria by Gardnerella vaginalis and other anaerobic bacteria. (journal of practical obstetrics and gynecology, 2020 (36) 5-358-361) BV is the most common vaginal infectious disease in women of child bearing age, the morbidity in China is 4.96% -36.3%, and the morbidity of women of 26-35 years old is the highest, and serious patients are often accompanied by complications such as pelvic inflammation, salpingitis, infertility, premature birth, premature rupture of fetal membranes, even HIV infection and the like. Gardnerella vaginalis (Gv) is the major and important pathogenic bacterium of BV.
At present, the treatment means of BV mainly comprises oral administration or local application of metronidazole or clindamycin, the recurrence rate of 2-3 months after treatment is 30% -50%, and the recurrence rate of 12 months after treatment is as high as 60% -80%. BV is diagnosed as Recurrent Bacterial Vaginosis (RBV) by 3 or more than 4 episodes per year. Recent research shows that the repeated BV attack is closely related to biofilm formation, and as Gardnerella vaginalis is excessively propagated, multi-microbial biofilms which are interacted among pathogenic bacteria mainly comprising Gardnerella vaginalis are formed in the vagina of BV patients, so that the sensitivity of antibacterial drug treatment is reduced, the drug resistance is increased, and RBV is generated.
2007, chinaScientists have discovered Fe 3 O 4 Magnetic nanoparticles have peroxidase-like activity and the enzymatic properties of nanomaterials, which mimic enzymes and are subsequently defined as nanoenzymes (nanozymes), have been studied systematically from an enzymatic point of view. In the aspect of antibiosis, the enzyme activity of different enzymes such as peroxidase and oxidase based on nano enzyme can remove bacterial biofilms, and further exert antibacterial activity.
Therefore, how to develop the nano enzyme into a medicinal preparation which can be applied to the antibacterial field, and more effectively kill or inhibit the growth and reproduction of gardnerella vaginalis, so as to develop a novel gardnerella vaginalis resistant preparation is urgent need of vast BV and RBV patients and urgent pursuit of medicinal research and development workers.
Disclosure of Invention
The inventor prepares the iron sulfide nano enzyme vaginal suppository on the basis of years of research work in the field of iron sulfide nano enzyme antibiosis. In the process of preparing and detecting the iron sulfide nanoenzyme pessary, the inventors surprisingly found that different types of gelatin play a significant role in the action of the matrix in the pessary, and also play a role in the activity of 'conditional pathogenic bacteria' of iron sulfide nanoenzyme such as gardnerella vaginalis, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and the like, namely: compared with the suppository matrix of type A gelatin, the iron sulfide nanoenzyme vaginal suppository prepared by using type B gelatin as the matrix has obvious advantages in establishing and maintaining normal flora balance of vagina, and further the inventor develops the iron sulfide nanoenzyme vaginal suppository for simply and efficiently treating or preventing bacterial vaginitis, bacterial Vaginosis (BV) and Recurrent Bacterial Vaginosis (RBV).
Firstly, the invention provides an iron sulfide nanoenzyme suppository, which comprises iron sulfide nanoenzyme and B type gelatin.
Specifically, the invention provides an iron sulfide nanoenzyme suppository, which comprises the following components in percentage by mass:
1) 0.01-1% of iron sulfide nano enzyme;
2) 10-39% of type B gelatin;
3) 60-89% of humectant.
Preferably, the iron sulfide nanoenzyme is Fe 3 S 4 Nanoenzymes or Fe 7 S 8 Nanoenzymes, all of which can be prepared using existing techniques, such as: preparation and application of ferroferric sulfide nano particles disclose Fe 3 S 4 Various methods for preparing nanoenzymes [2020, chemical bulletin (83) 10:897-908](ii) a The preparation of iron/carbon composite material and the research on its bacteriostatic property disclose Fe 7 S 8 Preparation method of nanosheet [2020 Master academic thesis of Yangzhou university]。
Preferably, wherein the content of the iron sulfide nanoenzyme is preferably 0.05-0.5%, more preferably 0.05-0.1%;
preferably, wherein the content of type B gelatin is preferably 15-20%, more preferably 20%.
Preferably, the humectant is one or a mixture of two or more of glycerin, propylene glycol, sorbitol and tween 80, and the content of the humectant is preferably 70-85%, and more preferably 70%.
Preferably, in order to improve shelf life, the iron sulfide nanoenzyme suppository of the present invention may further include a preservative, preferably, parabens such as methylparaben, ethylparaben, and the like.
In addition, the invention also provides a preparation method of the iron sulfide nanoenzyme suppository, which comprises the steps of swelling and softening type B gelatin by water (such as purified water and distilled water), mixing with other components, evaporating to remove water, standing, exhausting, molding and the like. Specifically, the method comprises the following steps:
1) Adding appropriate amount of water into type B gelatin, soaking until swelling and softening;
2) Adding the humectant, stirring uniformly, adding the ferric sulfide nanoenzyme, and stirring uniformly again;
3) Heating and evaporating on a magnetic stirrer to remove water;
4) Standing in a water bath to remove bubbles;
5) Injecting into suppository mold, molding, and taking out.
Furthermore, the invention provides an application of the iron sulfide nano enzyme vaginal suppository in preparing medicines for treating or preventing bacterial vaginitis, bacterial Vaginosis (BV) or Recurrent Bacterial Vaginosis (RBV). Wherein, the dosage of the ferric sulfide nano enzyme vaginal suppository can be 1-500 mg/day, preferably 5-100 mg/day, or determined according to factors such as the type, severity and the like of related diseases.
According to the current research result, although the regulation mechanism or action mechanism of the activity of the gelatin on the iron sulfide nano enzyme vaginal suppository against the conditioned pathogens such as gardnerella is not clear, the invention obtains the following beneficial effects based on the technical scheme:
(1) Compared with the type A gelatin, the iron sulfide nano enzyme vaginal suppository prepared by the type B gelatin has obviously improved activity against conditioned pathogens such as Gardnerella vaginalis and the like, hardly generates any inhibiting or killing effect on the growth and the reproduction of beneficial bacteria such as lactobacillus and the like, and is very favorable for quickly restoring vaginal flora to a normal balance state.
(2) The iron sulfide nano enzyme vaginal suppository prepared by the invention has the advantages of simple prescription and preparation process, low cost and the like, and has extremely high industrial application value.
Drawings
FIG. 1: the appearance of the iron sulfide nanoenzyme pessaries of the present invention prepared in example 1;
FIG. 2: electron microscopy scanning images of the iron sulfide nanoenzyme pessaries of the present invention prepared in example 1;
FIG. 3: photographs of the vaginal openings of mice in the experimental group and the control group;
FIG. 4: pathological section images of vaginal tissue of mice in experimental group and control group.
Detailed Description
The detailed description of the invention is provided only to illustrate the technical solutions that the invention can implement and should not be construed as limiting the invention in any way. Fe according to examples of the present invention, comparative examples, and the like 3 S 4 Nanoenzymes made by the inventors (example 9), fe 7 S 8 Nanometer enzyme ZuiyanThe iron/carbon composite material is prepared by a method disclosed in ' preparation of iron/carbon composite material and antibacterial performance research ' of university of state Master ' academic thesis, and other raw and auxiliary materials, reagents, instruments and equipment and the like are all purchased in the market.
EXAMPLES 1-4 preparation of the Fe3S4 Nanolase pessary of the present invention
1. Prescription
| Components | Example 1 | Example 2 | Example 3 | Example 4 |
| Iron sulfide nano enzyme | Fe 3 S 4 ，0.1g | Fe 3 S 4 ，1.0g | Fe 3 S 4 ，5.0g | Fe 3 S 4 ，10g |
| Type B gelatin | 100g | 150g | 200g | 300g |
| Glycerol | 600g | 700g | 750g | 800g |
| Purified water | 70g | 85g | 96g | 111g |
| Is made into | 450 granules | 550 granules | 650 granules | 800 granules |
2. Preparation method
(1) Gelatin is added to purified water and soaked for about 4 hours until swelling and softening.
(2) Adding glycerol, stirring, adding ferric sulfide nanometer enzyme, and stirring again.
(3) Heating and evaporating on a magnetic stirrer for 3-4 hours to remove water.
(4) Standing in 75 deg.C water bath for 1 hr to remove air bubbles.
(5) Injecting into a suppository mold coated with lubricant and precooled by 1.5g, slightly cooling, strickling, taking out after molding to obtain the Fe of the invention 3 S 4 A vaginal suppository containing nanometer enzyme. The appearance and scanning electron microscope images of the suppository prepared in example 1 are shown in fig. 1 and 2, respectively. The suppository prepared in examples 2-4 has an appearance similar to that of example 1 in an electron microscope scanning image.
Examples 5 to 8 Fe of the invention 7 S 8 Preparation method of nano enzyme vaginal suppository
The inventors prepared Fe according to the following recipe in the same preparation method as in examples 1-4 3 S 4 Fe for nano enzyme 7 S 8 Nano enzyme substitution, respectively preparingObtaining Fe with different specifications of the examples 5 to 8 of the invention 7 S 8 A vaginal suppository containing ferric sulfide nano enzyme. The appearance and scanning electron microscope images of the suppositories prepared in examples 5 to 8 were similar to those of example 1.
| Components | Example 5 | Example 6 | Example 7 | Example 8 |
| Iron sulfide nano enzyme | Fe 7 S 8 ，0.1g | Fe 7 S 8 ，1.0g | Fe 7 S 8 ，5.0g | Fe 7 S 8 ，10g |
| Type B gelatin | 100g | 150g | 200g | 300g |
| Glycerol | 600g | 700g | 750g | 800g |
| Purified water | 70g | 85g | 96g | 111g |
| Is made into | 450 granules | 550 granules | 650 granules | 800 granules |
Example 9Fe 3 S 4 Preparation method of nano enzyme
(1) 0.82g of FeCl 3 Dissolving in 40mL of ethylene glycol, stirring at room temperature for 30min, and performing ultrasonic treatment for 10min to ensure FeCl 3 And completely dissolving.
(2) 3.6g NaAc.3H was added 2 O, stirred at room temperature for 30 minutes.
(3) 1g of DADS was added and stirring was continued vigorously for 30 minutes.
(4) The mixture was transferred to a 50ml stainless steel autoclave, reacted at 200 ℃ for 12 hours, and naturally cooled to room temperature.
(5) Washing black precipitate with water and ethanol for 6 times, and vacuum drying at 60 deg.C for 5 hr to obtain Fe 3 S 4 And (3) nano enzyme.
Comparative examples 1-8 method for preparing iron sulfide nanoenzyme pessaries using type A gelatin
The inventors prepared Fe separately by replacing type B gelatin with type A gelatin according to the following formulation in the same manner as in examples 1 to 8 3 S 4 Nano enzyme pessary and Fe 7 S 8 A vaginal suppository containing nanometer enzyme. The appearance and scanning electron microscope images of the suppositories obtained in comparative examples 1 to 8 were similar to those of example 1.
Experimental example 1 antibacterial Activity of iron sulfide nanoenzyme vaginal suppository in vitro control experiment
According to the suppository inspection regulations of the four parts of Chinese pharmacopoeia (2020 edition), the inventor inspects the weight difference, the melting time limit and the microbial limit of all the iron sulfide nano enzyme vaginal suppositories prepared by the invention, and the results are in accordance with the regulations.
The inventors prepared Fe of examples 1 to 4 and comparative examples 1 to 4 3 S 4 Nanoenzyme pessaries and Fe prepared in examples 5-8 and comparative examples 5-8 7 S 8 Antibacterial activity control experiments are respectively carried out on the nano enzyme vaginal suppository, and the experimental method and the results are as follows:
1. experimental methods
The experimental example was carried out according to the method specified in "method for testing antibacterial properties of inorganic nanomaterial" (GB/T21510-2008) and appendix A thereof.
2. Results of the experiment
The results of comparative experiments (antibacterial rate%) on antibacterial activity of the iron sulfide nanoenzyme pessaries prepared in examples 1-8 and comparative examples 1-8 are as follows:
therefore, the iron sulfide nano enzyme vaginal suppository prepared by the type B gelatin can efficiently kill or inhibit 'conditional pathogenic bacteria', hardly influences the growth and reproduction of 'beneficial bacteria', and is remarkably superior to the iron sulfide nano enzyme vaginal suppository prepared by the type A gelatin in the aspect of establishing and maintaining vaginal flora balance in a comparison example.
Experimental example 2 evaluation of therapeutic Effect of iron sulfide Nanolase pessaries for vaginitis in mice
1. Experimental methods
(1) 7 weeks old female Balb/C mice, mice intravaginally inoculated with 10. Mu.L of 1X10 10 G. vagina lis (ATCC 14018) at CFU/ml, inoculated for 3 consecutive days.
(2) After successful infection, mice were randomized into three groups (5 per group): one experimental group and two control groups.
(3) Experimental group mice were given Fe prepared in Experimental example 1 3 S 4 Nano enzyme vaginal suppository, two control group mice respectively give control example 1 prepared Fe 3 S 4 The nano enzyme vaginal suppository and 10 mu L PBS are continuously treated for 3 days, and the treatment is carried out 1 time per day.
(4) The vaginal opening of the mice was photographed every other day.
(5) Mice were sacrificed 7d post infection and vaginal, uterine, heart, liver, spleen, lung, kidney and tissues were taken and fixed with formalin. And (3) performing hematoxylin-eosin (HE) staining analysis on the pathological section of the tissue. The tissue sections were observed using a Nikon Eclipse CI microscope in bright field mode.
2. Results of the experiment
The experimental results show that the Fe prepared in example 1 is compared with the PBS control group and the control example 1 3 S 4 The purulent secretion of mouse vaginal orifice after the treatment of the nano enzyme vaginal suppository is obviously reduced (figure 3), and inflammatory reactions such as infiltration of pathological lymphocytes and neutrophils in vaginal tissue, vascular hyperplasia and the like are obviously reduced (figure 4).
Other experimental results also show that Fe prepared in example 5 is comparable to PBS control, control 5 7 S 8 The purulent secretion of the mouse vaginal opening is obviously reduced after the nano enzyme vaginal suppository is used for treating, and inflammatory reactions such as pathological lymphocytes of vaginal tissues, neutrophil infiltration, vascular hyperplasia and the like are obviously reduced.
In addition, in order to further eliminate possible influences of manufacturers, product batches and the like on experimental results, the inventor conducts verification by using gelatin products of a plurality of different manufacturers and different batches. The validation experiment achieved similar results and conclusions as the control experiment described above.
Claims (10)
1. A suppository containing ferric sulfide nanoenzyme comprises ferric sulfide nanoenzyme and type B gelatin.
2. The suppository according to claim 1, which comprises the following components in percentage by mass:
1) 0.01-1% of iron sulfide nano enzyme;
2) 10-39% of type B gelatin;
3) 60-89% of humectant.
3. The suppository of claim 1 or 2 wherein the iron sulfide is Fe 3 S 4 Or Fe 7 S 8 。
4. The suppository of claim 1 or 2, wherein the iron sulfide nanoenzyme is present in an amount of 0.05-0.5%.
5. A suppository according to claim 1 or 2, wherein said type B gelatin is present in an amount of 15-20%.
6. The suppository according to claim 2, wherein said humectant is selected from one or a mixture of two or more of glycerin, propylene glycol, sorbitol, tween 80, in an amount of 70-85%.
7. The suppository of claim 1 or 2, further comprising a preservative.
8. The suppository of claim 7 wherein said preservative is a hydroxybenzoate.
9. A process for the preparation of a suppository as claimed in claim 1 or 2, comprising the steps of:
1) Adding appropriate amount of water into type B gelatin, soaking until swelling and softening;
2) Adding the humectant, uniformly stirring, adding the iron sulfide nano enzyme, and uniformly stirring again;
3) Heating and evaporating on a magnetic stirrer to remove water;
4) Standing in a water bath to remove bubbles;
5) Injecting into suppository mold, molding, and taking out.
10. Use of a suppository according to claim 1 or 2 for the preparation of a medicament for the treatment or prophylaxis of bacterial vaginosis, bacterial vaginosis or recurrent bacterial vaginosis.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115152897A (en) * | 2022-01-29 | 2022-10-11 | 河北金益合生物技术有限公司 | Iron sulfide nano enzyme feed additive and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180104386A (en) * | 2017-03-13 | 2018-09-21 | 동국대학교 산학협력단 | Mutifunctional bionanocomposite hydrogel, method of manufacturing thereof and use thereof |
| CN109260222A (en) * | 2018-08-31 | 2019-01-25 | 扬州大学 | Cysteine base antimicrobial nano vulcanizes iron mixture and its preparation method and application |
| CN109364100A (en) * | 2018-08-31 | 2019-02-22 | 扬州大学 | A kind of nanometer vulcanization iron mixture of high-efficiency antimicrobial and its preparation method and application |
| CN112791099A (en) * | 2021-01-12 | 2021-05-14 | 中国科学院生物物理研究所 | Pyrite nano-enzyme, anti-tumor medicine and application |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180104386A (en) * | 2017-03-13 | 2018-09-21 | 동국대학교 산학협력단 | Mutifunctional bionanocomposite hydrogel, method of manufacturing thereof and use thereof |
| CN109260222A (en) * | 2018-08-31 | 2019-01-25 | 扬州大学 | Cysteine base antimicrobial nano vulcanizes iron mixture and its preparation method and application |
| CN109364100A (en) * | 2018-08-31 | 2019-02-22 | 扬州大学 | A kind of nanometer vulcanization iron mixture of high-efficiency antimicrobial and its preparation method and application |
| CN112791099A (en) * | 2021-01-12 | 2021-05-14 | 中国科学院生物物理研究所 | Pyrite nano-enzyme, anti-tumor medicine and application |
Non-Patent Citations (3)
| Title |
|---|
| TING TONG等: "Antiviral Effects of Gelatin Stabilized Ferrous Sulfide Nanoparticles", 《RESEARCH SQUARE》, pages 1 - 18 * |
| 苏欣等: "纳米硫化铁对小鼠感染性湿疹模型的影响及机制研究", 《扬州大学学报(农业与生命科学版)》, no. 4, pages 67 - 73 * |
| 阴银燕等: "铁基纳米酶对鼠伤寒沙门菌生物被膜的影响", 《生物化学与生物物理进展》, vol. 46, no. 6, pages 587 - 595 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115152897A (en) * | 2022-01-29 | 2022-10-11 | 河北金益合生物技术有限公司 | Iron sulfide nano enzyme feed additive and preparation method and application thereof |
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