CN115166271A

CN115166271A - Sample adding needle device with preheating function

Info

Publication number: CN115166271A
Application number: CN202210775149.1A
Authority: CN
Inventors: 刘建; 吴冬; 苗新利; 吴国银
Original assignee: Suzhou Hybiome Biomedical Engineering Co Ltd
Current assignee: Suzhou Hybiome Biomedical Engineering Co Ltd
Priority date: 2022-07-01
Filing date: 2022-07-01
Publication date: 2022-10-11
Also published as: WO2024000729A1

Abstract

A loading needle assembly with preheat function, comprising: the sample adding needle comprises a needle point at the lower end, a needle tail at the upper end and a rod part in the middle; the heating sleeve is wrapped and arranged outside the sample adding needle; the heat insulation sleeve is wrapped on the outer side surface of the heating sleeve; the outer sleeve is wrapped on the outer side surface of the heat insulation insulating sleeve and made of conductive metal; the heating plate is arranged at the upper end part of the sample adding needle, and the heating sleeve penetrates through the heating plate; the heater is arranged on the top of the heating plate; and the wiring threaded sleeve is arranged at the upper end part of the sampling needle and is communicated with the heating sleeve. A heating device is designed on the sampling needle, and the heating surface of the sampling needle is controlled by regions, so that the sampling needle is uniformly heated in the whole length direction; the heating system has the functions of temperature control and over-temperature protection; has good heat preservation effect; the outmost layer is coated with metal, so that the sensitivity and accuracy of liquid level detection can be greatly improved.

Description

Sample adding needle device with preheating function

Technical Field

The application belongs to the technical field of medical instrument, especially, relate to a sample adding needle device with preheat function.

Background

During the test of the chemiluminescence immune device, the sample and the reagent need to be fully reacted in the environment of 37 +/-0.5 ℃, so that the antigen or antibody to be tested is fully captured. The reagent needs to be refrigerated, generally controlled at 2-8 ℃, the temperature of most samples is about 20 ℃, and the amount of the reagent is far larger than that of the samples. Most of the current devices place reaction containers (reaction cups) in an incubation tray, and heat is applied to the reaction cups by adding samples and reagents through a sample adding needle. The heating path is as follows: the heater heats the incubation tray body, and the incubation tray body heats the air in the tray, and the air heats the reaction cup, and the reaction cup heats sample and reagent again. The low heat transfer rate leads to a long time for raising the temperature of the sample and the reagent to 37 ℃, generally 5 to 10 minutes, and on the one hand, the reaction degree of the sample and the reagent is influenced; on the other hand, the time of the detection result is prolonged.

The application provides a sample adding needle with preheat function loads, and the design has heating device on the sample adding needle. The function of the sample adding needle is as follows: a certain amount of sample or reagent is sucked and added into the reaction vessel. The sample adding needle can quickly raise the temperature of the reagent or the sample to reach or approach 37 ℃ within a period of seconds between sample sucking and sample discharging, so that when the reagent or the sample cup is added into the reaction cup, the temperature can be quickly raised to 37 +/-0.5 ℃.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: the heat transfer rate is low, so that the time for heating the sample and the reagent to 37 ℃ is long, and the problem of low heating speed in the prior art is solved, so that the sample adding needle device with the preheating function is provided.

The technical scheme adopted by the invention for solving the technical problems is as follows: a sample adding needle device with a preheating function comprises:

the sample adding needle comprises a needle point at the lower end, a needle tail at the upper end and a middle rod part, and is used for sucking a certain amount of samples or reagents and adding the samples or the reagents into the reaction container;

the heating sleeve is wrapped and arranged outside the sample adding needle;

the heat insulation sleeve is wrapped on the outer side surface of the heating sleeve;

the outer sleeve is wrapped on the outer side surface of the heat insulation insulating sleeve and made of conductive metal;

the heating plate is arranged at the upper end part of the sample adding needle, and the heating sleeve penetrates through the heating plate;

the heater is arranged at the top of the heating plate and used for heating the heating plate;

and the wiring threaded sleeve is arranged at the upper end part of the sample adding needle and is in conduction connection with the outer sleeve.

Preferably, the sample adding needle device with the preheating function has a heat preservation upper cover arranged on the top of the heater.

Preferably, the heating plate is provided with a temperature-keeping cover on the bottom surface.

Preferably, according to the sample adding needle device with the preheating function, a temperature sensor and a temperature switch are mounted on the heating plate, and the temperature sensor is used for detecting the temperature of the heating plate.

Preferably, according to the sample adding needle device with the preheating function, the side wall of the outer sleeve is provided with a threaded wiring hole.

Preferably, according to the sample adding needle device with the preheating function, a first temperature equalizing groove is formed in the outer side wall of the upper end of the heating sleeve.

Preferably, according to the sample adding needle device with the preheating function, a second temperature equalizing groove is arranged on the outer side wall of the middle of the heating sleeve, and the length and width of the second temperature equalizing groove are smaller than those of the first temperature equalizing groove.

Preferably, according to the sample adding needle device with the preheating function, the outer side wall of the lower end part of the heating sleeve is provided with a smooth cylindrical surface.

Preferably, the sample adding needle device with the preheating function is provided with a first pouring sealant at the lower ports of the heating sleeve, the heat insulation insulating sleeve and the outer sleeve.

Preferably, according to the sample adding needle device with the preheating function, the second potting adhesive is arranged at the upper ports of the heat insulation sleeve and the outer sleeve, and the heating plate is isolated from the heat insulation sleeve and the outer sleeve by the second potting adhesive.

The invention has the beneficial effects that: a heating device is designed on the sample adding needle, and the temperature rise of the liquid is fast because the inner cavity of the sample adding needle is directly contacted with the liquid (sample or reagent);

the heating sleeve is provided with temperature equalizing grooves with various sizes, and the heating surface of the sampling needle is controlled by regions, so that the sampling needle is uniformly heated in the whole length direction;

the heating system has the functions of temperature control and over-temperature protection by installing the temperature sensor and the temperature switch on the heating plate;

the arrangement of the heat-preservation upper cover, the heat-preservation lower cover and the heat-insulation sleeve enables the device to have a good heat-preservation effect;

the outermost layer of this device increases the metal package, can greatly improve the sensitivity and the degree of accuracy of liquid level detection.

Drawings

The technical solution of the present application is further explained below with reference to the drawings and examples.

FIG. 1 is a schematic diagram of the overall structure of an embodiment of the present application;

FIG. 2 is a schematic and enlarged view of the internal structure of an embodiment of the present application;

FIG. 3 is a schematic view of a temperature equalizing structure of a heating jacket according to an embodiment of the present application.

The reference symbols in the figures are:

1: sample addition needle, 101: needle tip, 102: needle tail;

2: heating jacket, 201: first temperature equalizing groove, 202: a second temperature equalizing groove;

3: a heat insulating sleeve;

4: outer sleeve, 401: a wiring threaded hole;

5: a first pouring sealant;

6: second pouring sealant;

7: a heat preservation upper cover;

8: covering under heat preservation;

9: heating the plate;

10: a temperature sensor;

11: a temperature switch;

12: a heater;

13: and a wiring threaded sleeve.

Detailed Description

It should be noted that, in the present application, the embodiments and features of the embodiments may be combined with each other without conflict.

In the description of the present application, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the present application and to simplify the description, but are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed in a particular orientation, and be operated in a particular manner, and thus are not to be construed as limiting the scope of the present application. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate a number of the indicated technical features. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, the meaning of "a plurality" is two or more unless otherwise specified.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art through specific situations.

The technical solutions of the present application will be described in detail below with reference to the accompanying drawings in combination with embodiments.

Examples

The embodiment provides a sample application needle device with preheat function, refers to fig. 1, includes:

the sample adding needle 1 comprises a needle point 101 at the lower end, a needle tail 102 at the upper end and a rod part in the middle;

the sample adding needle 1 is configured into a long tube shape and is used for sucking a certain amount of liquid sample or reagent and discharging the liquid sample or reagent into a reaction cup. During liquid suction, the needle tip 101 directly enters the liquid level of the reagent tube to suck samples, and the needle tail end 102 is connected with a pump valve pipeline. To avoid the reaction between the sample injection needle 1 and the sample or the reagent, 316L stainless steel is generally used.

Referring to fig. 2, the heating jacket 2 wraps up the pole portion outside of setting in the middle of the application of sample needle 1, and the material that thermal conductivity is good is selected for use to heating jacket 2, generally selects pure copper material, and heating jacket 2 adopts silver solder with application of sample needle 1, welds the welding together with argon arc, and welded structure intensity is high, and the welding department heat conductivity is good, and heating jacket 2 conducts the heat for application of sample needle 1, carries out heating operation to application of sample needle 1. The lateral surface parcel at heating jacket 2 sets up thermal-insulated insulation support 3 to set up outer tube 4 in thermal-insulated insulation support 3 lateral surface parcel, outer tube 4 plays the guard action, and thermal-insulated insulation support 3 is used for keeping warm, makes heat conduction to application of sample needle 1 as much as possible on the heating jacket 2, and simultaneously, thermal-insulated function of thermal-insulated insulation support 3 makes outer tube 4 and heating jacket 2 isolated, can not scald the hand. The lower end openings of the heating sleeve 2, the heat insulation insulating sleeve 3 and the outer sleeve 4 and the upper end openings of the heat insulation insulating sleeve 3 and the outer sleeve 4 are filled and sealed by glue, and the heating plate 9 is isolated from the heat insulation insulating sleeve 3 and the outer sleeve 4 by the second filling and sealing glue 6 at the upper end, so that the heat insulation effect is achieved. The potting glue needs to be good in insulativity and high in bonding strength, such as epoxy resin glue.

Preferably, the sample adding needle device with the preheating function of this embodiment sets up heating plate 9 at sample adding needle 1 upper end, and sample adding needle 1, heating jacket 2 run through heating plate 9, have installed heater 12 above heating plate 9, and heater 12 chooses the thin film resistor type for use, and the surface has the gum, and is small simple to operate, and power density is big. During operation, the heater 12 heats the heating plate 9, the heating plate 9 transfers heat to the heating jacket 2, and the heating jacket 2 heats the sampling needle 1. After the sample or reagent is sucked by the sample adding needle 1, the liquid in the cavity is heated through the inner wall. Because the contact area of the inner wall of the cavity of the sample adding needle 1 and the liquid is large, the liquid amount sucked is relatively small, generally the maximum liquid amount is about 100uL, and the whole temperature rise process of the liquid is relatively fast.

Preferably, in the sample adding needle device with a preheating function of the present embodiment, the connection thread sleeve 13 is disposed at the upper end of the sample adding needle 1, and is welded to the heating needle 1, so that the connection is good, and the connection thread sleeve 13 is connected to one end of the liquid level detection line. The side wall of the outer sleeve 4 is provided with a wiring threaded hole 401, the wiring threaded hole 401 is welded with the outer sleeve 4, the conduction is good, and the wiring threaded hole 401 is connected with the other end shielding grounding end of the liquid level detection. Whether the needle tip contacts the liquid level is judged by detecting capacitance values at two ends. In this embodiment, the outer sleeve 4 wraps the outer periphery of the sample-sucking needle 1 to improve the sensitivity of small liquid volume detection, thereby reducing the dead volume of the sample-sucking.

Preferably, in the sample adding needle device with a preheating function of the present embodiment, the heat preservation upper cover 7 and the heat preservation lower cover 8 are respectively installed above and below the heating plate 9, so as to preserve heat of the whole heating system.

Preferably, in the sample adding needle device with a preheating function of the embodiment, the heating plate 9 is provided with the temperature sensor 10 and the temperature switch 11, the temperature sensor 10 can monitor the temperature of the heating plate 9, and when the temperature exceeds a set value, the heater stops or reduces the power to work; when the temperature is lower than the set value, the heater 12 works or works with increased power to generate heat. When the whole temperature control system is in failure, if the heater 12 is continuously operated, the temperature of the heating plate 9 is overhigh, and the switch of the temperature 11 is operated, so that the heater 12 stops operating.

Referring to fig. 3, preferably, in the sample adding needle device with a preheating function of this embodiment, a first temperature equalizing groove 201 is disposed on an outer side wall of an upper end portion (a region) of the heating jacket 2, and a second temperature equalizing groove 202 is disposed on an outer side wall (b region) of a middle portion of the heating jacket 2, in this embodiment, the first temperature equalizing groove 201 and the second temperature equalizing groove 202 are waist-shaped grooves, an outer side wall (c region) of a lower end portion of the heating jacket 2 is a smooth cylindrical surface, and a length and width dimension of the second temperature equalizing groove 202 is smaller than that of the first temperature equalizing groove 201. In order to reduce the non-uniformity of liquid heating, a plurality of temperature equalizing grooves are arranged at the higher temperature area of the heating jacket so as to reduce the contact heating area of the heating jacket and the sample adding needle. The first temperature equalizing groove 201 in the upper area is opened slightly larger and denser, the second temperature equalizing groove 202 in the middle area is opened second, and the temperature equalizing groove is not opened in the bottom area. Therefore, the temperature of the sampling needle can be distributed more uniformly, so that the liquid in the cavity of the sampling needle is heated uniformly.

In light of the foregoing description of the preferred embodiments according to the present application, it is to be understood that various changes and modifications may be made by those skilled in the art without departing from the scope of the invention as defined by the appended claims. The technical scope of the present application is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. The utility model provides a sample application needle device with preheat function which characterized in that includes:

the sample adding needle (1) comprises a needle point (101) at the lower end, a needle tail (102) at the upper end and a middle rod part, is used for sucking a certain amount of samples or reagents and is added into the reaction container;

the heating sleeve (2) is wrapped and arranged on the outer side of the sample adding needle (1);

the heat insulation insulating sleeve (3) is wrapped on the outer side surface of the heating sleeve (2);

the outer sleeve (4) is wrapped on the outer side surface of the heat insulation insulating sleeve (3), and the outer sleeve (4) is made of conductive metal;

the heating plate (9), the said heating plate (9) is set up in the upper end of the sample adding needle (1), and connect with hot sleeve (2);

the heater (12) is arranged at the top of the heating plate (9), and the heater (12) is used for heating the heating plate (9);

and the wiring threaded sleeve (13) is arranged at the upper end part of the sample adding needle (1) and is communicated and connected with the outer sleeve (4).

2. The sample adding needle device with preheating function as claimed in claim 1, wherein the top of the heater (12) is provided with a heat-insulating upper cover (7).

3. The sample adding needle device with preheating function as claimed in claim 2, wherein the heating plate (9) is provided with a heat-insulating cover (8) on the bottom surface.

4. The sample adding needle device with the preheating function is characterized in that a temperature sensor (10) and a temperature switch (11) are mounted on the heating plate (9), and the temperature sensor (10) is used for detecting the temperature of the heating plate (9).

5. The sample adding needle device with preheating function as claimed in claim 1, wherein the side wall of the outer sleeve (4) is provided with a threaded wiring hole (401).

6. The sample adding needle device with the preheating function according to any one of claim 2, wherein the outer side wall of the upper end of the heating jacket (2) is provided with a first temperature equalizing groove (201).

7. The sample adding needle device with the preheating function according to claim 6, wherein a second temperature equalizing groove (202) is arranged on the outer side wall of the middle part of the heating jacket (2), and the length and width of the second temperature equalizing groove (202) are smaller than those of the first temperature equalizing groove (201).

8. The sample adding needle device with the preheating function as claimed in claim 6, wherein the outer side wall of the lower end part of the heating jacket (2) is configured as a smooth cylindrical surface.

9. The sample adding needle device with the preheating function according to claim 8, wherein the lower ports of the heating sleeve (2), the heat insulating sleeve (3) and the outer sleeve (4) are provided with a first potting adhesive (5).

10. The sample adding needle device with the preheating function according to claim 9, wherein the upper ports of the heat insulating sleeve (3) and the outer sleeve (4) are provided with a second potting adhesive (6), and the second potting adhesive (6) isolates the heating plate (9) from the heat insulating sleeve (3) and the outer sleeve (4).