CN115166119A - High-precision low-pressure gradient method of high performance liquid chromatograph - Google Patents
High-precision low-pressure gradient method of high performance liquid chromatograph Download PDFInfo
- Publication number
- CN115166119A CN115166119A CN202210772961.9A CN202210772961A CN115166119A CN 115166119 A CN115166119 A CN 115166119A CN 202210772961 A CN202210772961 A CN 202210772961A CN 115166119 A CN115166119 A CN 115166119A
- Authority
- CN
- China
- Prior art keywords
- plunger pump
- liquid
- valve
- pressure
- plunger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 92
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000005213 imbibition Methods 0.000 claims abstract description 15
- 230000008569 process Effects 0.000 claims description 7
- 238000010521 absorption reaction Methods 0.000 claims description 6
- 238000005086 pumping Methods 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 230000004044 response Effects 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000003196 chaotropic effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
- G01N2030/342—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient fluid composition fixed during analysis
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The invention provides a high-precision low-pressure gradient method of a high-performance liquid chromatograph, which relates to the technical field of liquid chromatographs.A high-pressure pump adopts a series double-plunger pump, the liquid suction side of a first plunger pump and the liquid suction side of a second plunger pump are respectively provided with an electromagnetic valve and a one-way valve, the electromagnetic valve is used for opening liquid flow entering the direction of the first plunger pump from a liquid suction inlet, and the one-way valve is used for controlling the liquid flow entering the direction of the second plunger pump from the first plunger pump; before the first plunger pump does not suck, the electromagnetic valve is opened, so that the pressure of the first plunger pump is reduced to the atmospheric pressure, no delay imbibition is realized when the first plunger pump starts sucking, the difference of mixing ratio precision caused by the delay of opening the one-way valve is avoided, the imbibition time of a small proportion is increased by designing a symmetrical imbibition interval, the imbibition precision of the small proportion is improved, and the analysis precision of the high performance liquid chromatograph is further improved.
Description
Technical Field
The invention relates to a high-performance liquid chromatograph, in particular to a high-precision low-pressure gradient method of the high-performance liquid chromatograph.
Background
The high performance liquid chromatograph is an apparatus that uses the principle of chromatographic separation, and injects a sample solution into a mobile phase injected by a sample injector by pushing the mobile phase into a system by a high-pressure pump, thereby separating the mobile phase on a chromatographic column and analyzing each component by a detector. The high pressure pump commonly used in the industry at present is a series double-plunger pump driven by a cam driven by a motor. According to the designed cam curve, the circumferential rotation of the cam can be converted into liquid suction and liquid pushing actions of the plunger rod. And then the double-plunger pump is matched to form a continuous liquid suction and liquid delivery process with relatively stable system pressure.
The process of imbibing under low pressure, and absorbing different mobile phases according to a certain proportion is called as a low pressure gradient mode. The low-pressure gradient mode generally forms mixed mobile phases with different proportions according to the opening and closing time of the gradient valve. The mixing precision of the mixed mobile phase, i.e. the low pressure gradient mixing ratio precision, has a direct influence on the final analysis result, and the higher the low pressure gradient mixing ratio precision is, the higher the accuracy of the analysis result is. Therefore, it is necessary to improve the low pressure gradient mixing ratio accuracy as much as possible when performing component analysis by a high performance liquid chromatograph.
However, in the conventional hplc, the mixing ratio accuracy of the low pressure gradient is not high for various reasons, for example, since the check valve is not opened before the pressure in the pump is reduced to the normal pressure when the plunger starts the sucking action, and the mobile phase cannot be sucked into the pump, the theoretical sucking time and the time sucking time are different, which may cause poor mixing accuracy of the mixed mobile phase. As another example, when the mixing ratio is small (for example, less than 5%), the gradient valve response time is insufficient, and the mixing accuracy of the small ratio is deteriorated. In addition, because the types of flow phases are various, the volume elastic coefficients of the flow phases are different, the opening time of the check valve has different values, the starting time of the check valve is different due to the change of other factors such as different system pressures, different temperatures and the like, although the prior art corrects the mixing time of the mobile phase of the system pressure, the prior art cannot correspond to different temperatures and mobile phases, and the generated error is large.
Disclosure of Invention
In view of the above, the present invention provides a high-precision low-pressure gradient method for a high performance liquid chromatograph, so as to improve the mixing ratio precision of the low-pressure gradient, and further improve the analysis precision of the high performance liquid chromatograph.
Therefore, the invention provides the following technical scheme:
a high-precision low-pressure gradient method of a high-performance liquid chromatograph is characterized in that a high-pressure pump in the high-performance liquid chromatograph adopts a series double-plunger pump, a liquid suction side of a first plunger pump and a liquid suction side of a second plunger pump are respectively provided with an electromagnetic valve and a one-way valve, the electromagnetic valves are used for opening liquid flow entering the direction of the first plunger pump from a liquid suction port, and the one-way valves are used for controlling the liquid flow entering the direction of the second plunger pump from the first plunger pump;
before the first plunger pump sucks no air, the electromagnetic valve is opened, so that the pressure of the first plunger pump is reduced to the atmospheric pressure.
Further, the solenoid valve is a 2-way solenoid valve.
Further, the small-proportion imbibing interval is designed to be symmetrical front and back.
Further, the time for small-scale pipetting is increased when the corresponding gradient scale is changed.
Further, a small-proportion liquid absorption interval pair strain gradient model which is symmetrical front and back is adopted.
Further, the pipetting process of the first plunger pump is as follows:
when the first plunger piston finishes pumping liquid, the first plunger piston stops moving, the electromagnetic valve and the gradient valve are opened, the system pressure is reduced to the atmospheric pressure, and the one-way valve is automatically closed;
the first plunger rod uniformly accelerates liquid absorption, absorbs a small proportion of mobile phase, and increases the valve opening time of the small proportion of mixing ratio;
the first plunger rod absorbs liquid at a constant speed, so that the liquid absorption speed is increased;
the first plunger rod absorbs liquid at a uniform speed, absorbs a small proportion of mobile phase, and increases the valve opening time of a small proportion of mixing ratio;
and after liquid suction is finished, the electromagnetic valve and the gradient valve are closed simultaneously to wait for liquid delivery.
The invention has the advantages and positive effects that:
1. the scheme of the invention changes the one-way valve into the 2-way electromagnetic valve, can relieve pressure in advance, is not influenced by various factors such as pressure, temperature and flow, can realize non-delay imbibition, and improves imbibition precision.
2. In the invention, on the basis of realizing non-delay imbibition, the imbibition interval is set as a symmetrical imbibition interval, so that the accuracy of small-proportion (for example, less than 5%) imbibition can be improved, and the separation performance and consistency of the hue chromatograph are further improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic diagram of the position of a high pressure pump in a liquid chromatograph according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a tandem dual plunger pump according to an embodiment of the present invention;
FIG. 3 is a block diagram of a high pressure pump control according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a cam speed regulation interval curve according to an embodiment of the present invention;
FIG. 5 is a graphical illustration of the cam pumping interval in an embodiment of the present invention;
FIG. 6 is an exemplary graph of varying gradients in an embodiment of the invention.
Detailed Description
In order to make those skilled in the art better understand the technical solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, shall fall within the protection scope of the present invention.
It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those illustrated or described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
As shown in fig. 1, the high performance liquid chromatograph includes: the device comprises a high-pressure pump, a sample injector, a column incubator and a detector; wherein: the high-pressure pump adopts a series double-plunger pump, the motor drives a cam shaft to rotate through a belt, and the cam shaft is provided with 2 cams which respectively drive plunger rods of the 2 plunger pumps to push and pull. As shown in fig. 2, the driving motor 1 rotates to drive the camshaft 3 to rotate through the transmission belt 2; two cams (a first cam 4 and a second cam 5) on the cam shaft synchronously rotate to respectively drive two plunger rods (a first plunger rod 6 and a second plunger rod 7) to reciprocate; the plunger rod is drawn out and pushed in the plunger pump, and liquid is sucked in from the liquid suction port and discharged from the liquid discharge port 14 under the control of the gradient valve 13, so that liquid suction and liquid pushing effects are achieved. An electromagnetic valve 10 and a check valve 11 are respectively arranged on the liquid suction side of the first plunger pump 8 and the liquid suction side of the second plunger pump 9, the electromagnetic valve 10 is used for opening the liquid flow entering the first plunger pump 8 from the liquid suction inlet, and the check valve 11 can only enter the liquid flow entering the second plunger pump 9 from the first plunger pump 8. The liquid outlet of the second plunger pump 9 is connected with a pressure sensor 12 which can monitor the pressure in the rear pipeline.
In the existing high performance liquid chromatograph, a check valve is arranged on the liquid suction side of a first plunger pump 8, and in the liquid suction process of the first plunger pump 8, when suction is started, the pressure of the first plunger pump 8 is system pressure, and is different from atmospheric pressure (0.1 MPa), so that the check valve cannot be opened immediately, and the precision of the mixing ratio of mobile phases is poor.
In contrast, in the present embodiment, the check valve provided on the suction side of the first plunger pump 8 is replaced with a 2-way solenoid valve, and the 2-way solenoid valve is opened to reduce the pressure of the first plunger pump 8 to the atmospheric pressure before the first plunger pump 8 sucks no liquid. When the first plunger pump 8 starts to suck, the liquid is sucked without delay, the liquid is not influenced by various factors such as pressure, temperature and flow, and the difference of mixing ratio precision caused by the delay of opening the one-way valve is avoided.
When the hplc is controlled, as shown in fig. 3, the control system drives the motor to rotate, drives the transmission mechanisms such as the cam shaft in the high-pressure pump mechanism to pump out the separation liquid from the liquid storage bottle, and the separation liquid flows into the rear end through the pressure sensor, the pressure sensor collects the system pressure and feeds the system pressure back to the control system, and then the motor is controlled to adjust the rotation speed at a proper time.
In order to further improve the low-pressure gradient mixing ratio accuracy of a high-performance liquid chromatograph, in the embodiment of the invention, a check valve arranged on the liquid suction side of a first plunger pump 8 is changed into a 2-way solenoid valve, a liquid suction interval with symmetrical front and back is designed, and when the gradient ratio is changed, the time of a small ratio is increased, so that the accuracy of the small ratio is improved.
As shown in fig. 4, according to the design of the cam speed regulation curve, theoretically, the motor driving the cam shaft rotates at a constant speed, the first plunger pump 8 and the second plunger pump 9 alternately suck and push liquid, and the liquid can be continuously pushed at a constant speed to the rear end. As shown in fig. 5:
stage (1): when the first plunger pump 8 finishes liquid feeding, the first plunger rod 6 stops moving, the electromagnetic valve 10 and the gradient valve 13 are opened, and the system pressure is reduced to the atmospheric pressure. The check valve 11 closes automatically, avoiding mixing ratio accuracy deviations.
Stage (2): the plunger rod starts to suck liquid.
Stage (3): and after the liquid suction is finished, the electromagnetic valve and the gradient valve are closed simultaneously to wait for liquid delivery.
On the other hand, in the case of a small-proportion mixed mobile phase, the liquid-feeding time is too short, and the time for closing the valve is too long, so that the liquid-suction accuracy is not accurate. In the embodiment of the invention, the small-proportion imbibition precision is improved by increasing the small-proportion imbibition time (the increased time is calculated according to the designed imbibition interval). In addition, the small-proportion imbibition interval can be designed to be symmetrical front and back, and for a variable gradient model, the variable gradient means: the A chaotropic agent was changed from 100% to 0% and the B chaotropic agent was changed from 0% to 100% in a fixed time, as shown in FIG. 6. Specifically, the method comprises the following steps:
as shown in fig. 5, according to the design of the cam liquid suction curve, theoretically, the motor driving the cam shaft rotates at a constant speed, and the first plunger pump 8 and the second plunger pump 9 alternately suck and push liquid, so that the liquid can be continuously pushed to the rear end at a constant speed.
Stage (1): when the first plunger pump 8 finishes liquid feeding, the first plunger rod 6 stops moving, the electromagnetic valve and the gradient valve are opened, the system pressure is reduced to the atmospheric pressure, and the one-way valve is automatically closed.
Stage (2): the first plunger rod 6 uniformly accelerates liquid absorption, absorbs a small proportion of mobile phase, increases the valve opening time of a small proportion mixing ratio, and improves the precision of small proportion mixing.
Stage (3): the first plunger rod 6 sucks liquid at a constant speed, and the liquid sucking speed is increased.
Stage (4): the first plunger rod 6 uniformly decelerates to absorb liquid, absorbs a small proportion of mobile phase, increases the valve opening time of a small proportion mixing ratio, and improves the precision of small proportion mixing.
Stage (5): and after liquid suction is finished, the electromagnetic valve and the gradient valve are closed simultaneously to wait for liquid delivery.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (6)
1. A high-precision low-pressure gradient method of a high-performance liquid chromatograph is characterized in that a high-pressure pump in the high-performance liquid chromatograph adopts a series double-plunger pump, a liquid suction side of a first plunger pump and a liquid suction side of a second plunger pump are respectively provided with an electromagnetic valve and a one-way valve, the electromagnetic valves are used for opening liquid flow entering the direction of the first plunger pump from a liquid suction port, and the one-way valves are used for controlling the liquid flow entering the direction of the second plunger pump from the first plunger pump;
before the first plunger pump sucks the air, the electromagnetic valve is opened, so that the pressure of the first plunger pump is reduced to the atmospheric pressure.
2. The high accuracy low pressure gradient method of high performance liquid chromatograph of claim 1, wherein the solenoid valve is a 2-way solenoid valve.
3. The method of claim 1, wherein the small-fraction pipetting zones are designed to be symmetrical in the front-to-back direction.
4. The method of claim 3, wherein the time for small-scale pipetting is increased in response to a change in the gradient scale.
5. The method of claim 3, wherein the small-scale imbibition space is symmetric with respect to the strain gradient model.
6. The high-precision low-pressure gradient method for high performance liquid chromatograph of claim 4, wherein the imbibing process of the first plunger pump is as follows:
when the first plunger piston finishes pumping liquid, the first plunger piston stops moving, the electromagnetic valve and the gradient valve are opened, the system pressure is reduced to the atmospheric pressure, and the one-way valve is automatically closed;
the first plunger rod uniformly accelerates liquid absorption, absorbs a small proportion of mobile phase and increases the valve opening time of the small proportion of mixing ratio;
the first plunger rod absorbs liquid at a constant speed, so that the liquid absorption speed is increased;
the first plunger rod uniformly decelerates to absorb liquid, absorbs a small proportion of mobile phase, and increases the valve opening time of the small proportion of mixing ratio;
and after liquid suction is finished, the electromagnetic valve and the gradient valve are closed simultaneously to wait for liquid delivery.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210772961.9A CN115166119A (en) | 2022-06-30 | 2022-06-30 | High-precision low-pressure gradient method of high performance liquid chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210772961.9A CN115166119A (en) | 2022-06-30 | 2022-06-30 | High-precision low-pressure gradient method of high performance liquid chromatograph |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115166119A true CN115166119A (en) | 2022-10-11 |
Family
ID=83489378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210772961.9A Pending CN115166119A (en) | 2022-06-30 | 2022-06-30 | High-precision low-pressure gradient method of high performance liquid chromatograph |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115166119A (en) |
Citations (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4128476A (en) * | 1977-06-14 | 1978-12-05 | Spectra-Physics, Inc. | Carrier composition control for liquid chromatographic systems |
| US5852231A (en) * | 1996-03-29 | 1998-12-22 | Hitachi, Ltd. | Elution liquid control for a liquid chromatograph |
| US20040164013A1 (en) * | 2002-11-01 | 2004-08-26 | Kunihiko Takao | Pump for liquid chromatograph |
| US20050023205A1 (en) * | 2003-08-01 | 2005-02-03 | Kenji Hiraku | Pump for liquid chromatography |
| US20050084386A1 (en) * | 2002-03-18 | 2005-04-21 | Kiyotoshi Mori | Gradient liquid feed pump system, and liquid chromatograph |
| JP2007327846A (en) * | 2006-06-07 | 2007-12-20 | Gl Sciences Inc | Gradient liquid feeding method and device |
| JP2008291848A (en) * | 2008-08-04 | 2008-12-04 | Hitachi High-Technologies Corp | Pump for liquid chromatography |
| JP2010014453A (en) * | 2008-07-02 | 2010-01-21 | Hitachi High-Technologies Corp | Liquid sending device and liquid chromatograph |
| CN201696242U (en) * | 2010-04-22 | 2011-01-05 | 杭州奥希科技有限公司 | Double-plunger high-pressure infusion pump for liquid chromatograph |
| US20130104631A1 (en) * | 2010-07-29 | 2013-05-02 | Kenichiro Tokuo | Pump for liquid chromatograph, and liquid chromatograph |
| WO2014030498A1 (en) * | 2012-08-23 | 2014-02-27 | 株式会社 日立ハイテクノロジーズ | Liquid chromatography device and liquid delivery device |
| CN103869029A (en) * | 2012-12-18 | 2014-06-18 | 北京普源精电科技有限公司 | Liquid chromatograph with reciprocating in-series plunger pump |
| CN104101658A (en) * | 2013-04-10 | 2014-10-15 | 北京普源精电科技有限公司 | High performance liquid chromatograph controllable in flow velocity |
| CN104101669A (en) * | 2013-04-10 | 2014-10-15 | 北京普源精电科技有限公司 | High performance liquid chromatograph used for control of system pressure pulsation |
| JP2015001456A (en) * | 2013-06-17 | 2015-01-05 | 株式会社日立ハイテクノロジーズ | Liquid feeder for liquid chromatograph and liquid chromatography device |
| CN104730180A (en) * | 2013-12-18 | 2015-06-24 | 北京普源精电科技有限公司 | Liquid chromatograph having mixing ratio correcting function |
| CN104730179A (en) * | 2013-12-18 | 2015-06-24 | 北京普源精电科技有限公司 | Liquid chromatograph capable of controlling mobile phase mixing ratio |
| CN106018638A (en) * | 2016-05-17 | 2016-10-12 | 大连依利特分析仪器有限公司 | Liquid chromatogram multielement mobile phase low-pressure gradient control method |
| CN109444310A (en) * | 2018-10-12 | 2019-03-08 | 安徽皖仪科技股份有限公司 | The high pressure resistant gradient valve of liquid chromatogram and liquid chromatogram feed liquor system |
| CN111812262A (en) * | 2020-07-17 | 2020-10-23 | 依利特(苏州)分析仪器有限公司 | Liquid chromatography multi-element low-pressure low-proportion control method and device |
| CN211905239U (en) * | 2020-04-09 | 2020-11-10 | 苏州康纯医药科技有限公司 | High-pressure delivery pump of liquid chromatograph for detecting surfactant |
| CN112107885A (en) * | 2020-09-17 | 2020-12-22 | 山东悟空仪器有限公司 | Control method and device for quaternary low-pressure gradient pump |
-
2022
- 2022-06-30 CN CN202210772961.9A patent/CN115166119A/en active Pending
Patent Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4128476A (en) * | 1977-06-14 | 1978-12-05 | Spectra-Physics, Inc. | Carrier composition control for liquid chromatographic systems |
| US5852231A (en) * | 1996-03-29 | 1998-12-22 | Hitachi, Ltd. | Elution liquid control for a liquid chromatograph |
| US20050084386A1 (en) * | 2002-03-18 | 2005-04-21 | Kiyotoshi Mori | Gradient liquid feed pump system, and liquid chromatograph |
| US20040164013A1 (en) * | 2002-11-01 | 2004-08-26 | Kunihiko Takao | Pump for liquid chromatograph |
| US20050023205A1 (en) * | 2003-08-01 | 2005-02-03 | Kenji Hiraku | Pump for liquid chromatography |
| JP2007327846A (en) * | 2006-06-07 | 2007-12-20 | Gl Sciences Inc | Gradient liquid feeding method and device |
| JP2010014453A (en) * | 2008-07-02 | 2010-01-21 | Hitachi High-Technologies Corp | Liquid sending device and liquid chromatograph |
| JP2008291848A (en) * | 2008-08-04 | 2008-12-04 | Hitachi High-Technologies Corp | Pump for liquid chromatography |
| CN201696242U (en) * | 2010-04-22 | 2011-01-05 | 杭州奥希科技有限公司 | Double-plunger high-pressure infusion pump for liquid chromatograph |
| US20130104631A1 (en) * | 2010-07-29 | 2013-05-02 | Kenichiro Tokuo | Pump for liquid chromatograph, and liquid chromatograph |
| WO2014030498A1 (en) * | 2012-08-23 | 2014-02-27 | 株式会社 日立ハイテクノロジーズ | Liquid chromatography device and liquid delivery device |
| CN104508478A (en) * | 2012-08-23 | 2015-04-08 | 株式会社日立高新技术 | Liquid chromatography device and liquid delivery device |
| CN103869029A (en) * | 2012-12-18 | 2014-06-18 | 北京普源精电科技有限公司 | Liquid chromatograph with reciprocating in-series plunger pump |
| CN104101658A (en) * | 2013-04-10 | 2014-10-15 | 北京普源精电科技有限公司 | High performance liquid chromatograph controllable in flow velocity |
| CN104101669A (en) * | 2013-04-10 | 2014-10-15 | 北京普源精电科技有限公司 | High performance liquid chromatograph used for control of system pressure pulsation |
| JP2015001456A (en) * | 2013-06-17 | 2015-01-05 | 株式会社日立ハイテクノロジーズ | Liquid feeder for liquid chromatograph and liquid chromatography device |
| CN104730180A (en) * | 2013-12-18 | 2015-06-24 | 北京普源精电科技有限公司 | Liquid chromatograph having mixing ratio correcting function |
| CN104730179A (en) * | 2013-12-18 | 2015-06-24 | 北京普源精电科技有限公司 | Liquid chromatograph capable of controlling mobile phase mixing ratio |
| CN106018638A (en) * | 2016-05-17 | 2016-10-12 | 大连依利特分析仪器有限公司 | Liquid chromatogram multielement mobile phase low-pressure gradient control method |
| CN109444310A (en) * | 2018-10-12 | 2019-03-08 | 安徽皖仪科技股份有限公司 | The high pressure resistant gradient valve of liquid chromatogram and liquid chromatogram feed liquor system |
| CN211905239U (en) * | 2020-04-09 | 2020-11-10 | 苏州康纯医药科技有限公司 | High-pressure delivery pump of liquid chromatograph for detecting surfactant |
| CN111812262A (en) * | 2020-07-17 | 2020-10-23 | 依利特(苏州)分析仪器有限公司 | Liquid chromatography multi-element low-pressure low-proportion control method and device |
| CN112107885A (en) * | 2020-09-17 | 2020-12-22 | 山东悟空仪器有限公司 | Control method and device for quaternary low-pressure gradient pump |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20230135114A1 (en) | Sample Injector With Metering Device Balancing Pressure Differences In An Intermediate Valve State | |
| US7350401B2 (en) | Liquid feeding system | |
| US8160751B2 (en) | Devices, systems and methods for flow-compensating pump-injector synchronization | |
| US4310420A (en) | Mobile phase supplying method in the liquid chromatography and apparatus therefor | |
| CN103038632B (en) | Pump for liquid chromatograph, and liquid chromatograph | |
| US20140338431A1 (en) | Methods for Injecting Samples in Liquid Chromatography, Particularly in High Performance Liquid Chromatography | |
| US8191405B2 (en) | Solvent delivery device and analytical system | |
| US20180274531A1 (en) | Liquid feed device, liquid feed control method for liquid feed device, and liquid feed control program for liquid feed device | |
| US5852231A (en) | Elution liquid control for a liquid chromatograph | |
| JP4732960B2 (en) | Gradient liquid feeding method and apparatus | |
| CN103590993A (en) | Method for reducing flow fluctuations in liquid chromatograph and infusion pump device | |
| US20120055581A1 (en) | Liquid delivery devide and liquid chromatography device | |
| WO2014030498A1 (en) | Liquid chromatography device and liquid delivery device | |
| CN110809713A (en) | Liquid feeding device and fluid chromatograph | |
| CN115166119A (en) | High-precision low-pressure gradient method of high performance liquid chromatograph | |
| US11808252B2 (en) | Liquid delivery device and liquid chromatograph | |
| CN108167171B (en) | A kind of control method that infusion pump pressure fluctuation automatically corrects | |
| CN115190942A (en) | Liquid feeding device and liquid feeding method | |
| CN111812262B (en) | Liquid chromatography multielement low-pressure low-proportion control method and device | |
| JP2504001B2 (en) | Liquid transfer device | |
| JP2006118374A (en) | Liquid feeding system | |
| CN111812261A (en) | Multi-mode chromatography solvent management system and control method thereof | |
| JP2023117988A (en) | Liquid chromatograph | |
| CN109444448A (en) | A kind of liquid continuous sample inlet system and continuous sample introduction method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20240115 Address after: 101407 101, Zone 4, 1st Floor, Building 1, No. 6, Yanqi South 4th Street, Yanqi Economic Development Zone, Huairou District, Beijing Applicant after: China spectrum Tech (Beijing) Technology Co.,Ltd. Address before: 116000 No. 125, Gaoneng street, high tech Industrial Park, Dalian, Liaoning Applicant before: Kunpeng instrument (Dalian) Co.,Ltd. |