CN115165836A - Method for detecting common drugs in hair - Google Patents
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- CN115165836A CN115165836A CN202210978031.9A CN202210978031A CN115165836A CN 115165836 A CN115165836 A CN 115165836A CN 202210978031 A CN202210978031 A CN 202210978031A CN 115165836 A CN115165836 A CN 115165836A
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- 210000004209 hair Anatomy 0.000 title claims abstract description 66
- 229940079593 drug Drugs 0.000 title claims abstract description 42
- 239000003814 drug Substances 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 29
- 231100000640 hair analysis Toxicity 0.000 claims abstract description 47
- 238000001514 detection method Methods 0.000 claims abstract description 39
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000006166 lysate Substances 0.000 claims abstract description 13
- 239000011259 mixed solution Substances 0.000 claims abstract description 10
- 239000011324 bead Substances 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims description 24
- 238000005070 sampling Methods 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- 238000010166 immunofluorescence Methods 0.000 claims description 10
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 6
- 229910052782 aluminium Inorganic materials 0.000 claims description 6
- 239000011888 foil Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 210000004761 scalp Anatomy 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 108010067770 Endopeptidase K Proteins 0.000 claims description 3
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 238000012864 cross contamination Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 3
- 239000008213 purified water Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 101100008047 Caenorhabditis elegans cut-3 gene Proteins 0.000 claims description 2
- 210000003128 head Anatomy 0.000 claims description 2
- 208000011117 substance-related disease Diseases 0.000 abstract description 8
- 206010013654 Drug abuse Diseases 0.000 abstract description 6
- 238000012216 screening Methods 0.000 abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 4
- 241001622623 Coeliadinae Species 0.000 abstract description 2
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 238000000338 in vitro Methods 0.000 abstract description 2
- 238000007689 inspection Methods 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract description 2
- 230000007115 recruitment Effects 0.000 abstract 1
- 239000004005 microsphere Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- BQJCRHHNABKAKU-KBQPJGBKSA-N morphine Chemical compound O([C@H]1[C@H](C=C[C@H]23)O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4O BQJCRHHNABKAKU-KBQPJGBKSA-N 0.000 description 4
- 108010008038 Synthetic Vaccines Proteins 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 210000003296 saliva Anatomy 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 206010013663 drug dependence Diseases 0.000 description 2
- 229960005181 morphine Drugs 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- KWTSXDURSIMDCE-QMMMGPOBSA-N (S)-amphetamine Chemical compound C[C@H](N)CC1=CC=CC=C1 KWTSXDURSIMDCE-QMMMGPOBSA-N 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 208000007271 Substance Withdrawal Syndrome Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229940025084 amphetamine Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 238000000589 high-performance liquid chromatography-mass spectrometry Methods 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 229960001252 methamphetamine Drugs 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- Life Sciences & Earth Sciences (AREA)
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- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract
The invention discloses a method for detecting common drugs in hair, which comprises the following steps: s1, collecting a hair sample; s2, hair sample treatment: mixing and grinding a hair sample, a hair lysate and zirconia grinding beads to obtain a mixed solution; s3, detecting a hair sample: the invention is suitable for the technical field of reagent in-vitro diagnosis, designs a method which has simple operation, short inspection time and low cost, is not limited by places and sexes, can quickly, simply and conveniently detect whether an examinee sucks the drugs, is suitable for the primary screening quantitative detection of the hair of a suspect with unknown drug abuse by drug ban, public security policemen and drug rehabilitation departments, is also suitable for the primary screening of the drugs or the drug abuse and the like by a health epidemic prevention system, customs detection, soldier experience, physical examination in recruitment, government officer physical examination and sensitive department physical examination, and is suitable for basic popularization and application.
Description
Technical Field
The invention belongs to the technical field of reagent in-vitro diagnosis, and particularly relates to a method for detecting common drugs in hair.
Background
Drug abuse is a major problem that seriously jeopardizes social safety and human life health, and drug addicts are on a trend of increasing quantity and becoming younger. At present, the immune colloidal gold technology, the gas chromatography-mass spectrometry and the high performance liquid chromatography-mass spectrometry are mainly applied at home and abroad to detect and identify the blood, saliva, urine and hair samples of the virus-absorbing personnel. The time of the blood and saliva sample detection window is 30 minutes to several days, the time of the urine sample detection window is 2 hours to 7 days, and the time of the hair sample detection window is one week to several years. Typically, 24-48 hours after drug withdrawal, blood, urine and saliva drugs are essentially metabolically excreted, resulting in negative tests that do not provide valuable information.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for detecting common drugs in hair.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for detecting common drugs in hair comprises the following steps:
s1, collecting a hair sample;
s2, hair sample treatment: mixing and grinding a hair sample, a hair lysate and zirconia grinding beads to obtain a mixed solution;
s3, detecting a hair sample: and taking the supernatant of the mixed solution, adding the sample, timing, and detecting to obtain the content of common drugs.
Preferably, in step S1, when the sample of the hair of the person to be detected is extracted, the worker should wear a disposable glove, and use medical scissors or saw-tooth scissors to cut the hair with a length of the back of the vertex of less than 3 cm and a length of the hair longer than 3 cm tightly against the surface of the scalp of the person to be extracted, cut 3 cm from the end of the hair root, and select the head part nearest to the back of the vertex if the back of the vertex cannot extract enough hair.
Preferably, in step S1, the extracted hair sample is divided into two parts, a and B, each part of the hair sample is not less than 50 mg, the hair sample is wrapped by aluminum foil paper, the hair sample is respectively packaged in material evidence bags, the material evidence bags are packaged, information such as sample numbers, extraction dates and extractors are filled in the material evidence bags, the seal of the material evidence bags is identified by the extracted person according to fingerprints and signing, the extracted person refuses to press fingerprints or sign, the extractor is noted, and the whole extraction process is recorded.
Preferably, in the step S1, the hair extraction worker creates a hair sample extraction information table in which information such as the name of the person to be extracted, the number of the person who is resident to be extracted, the type of hair to be extracted, the extraction location, the extraction unit, the extraction worker, and the extraction time is described.
Preferably, in the step S1, the hairs of different persons are respectively extracted, packaged independently, numbered uniformly, and residues on the extraction equipment in the sampling process are cleaned in time to prevent cross contamination.
Preferably, in step S2, the hair sample treatment includes:
weighing 20mg of hair sample to be tested, placing into a 5mL sampling tube, grinding with a portable grinder at 5000rpm for 3 minutes, wherein the 5mL sampling tube contains 2mL of 0.61% tris of 0.5mg/mL proteinase K; 0.85% NACL;1.0% tween-20;0.1% of hair lysate prepared from proclin300 purified water and 95% of zirconia grinding beads having a particle size of 2-5 mm.
Preferably, in step S3, the hair sample detection includes:
s31, starting the immunofluorescence analyzer, and inserting and reading the ID cards corresponding to the batch of products;
s32, tearing the aluminum foil bag, taking out the detection card, and horizontally placing the detection card on an experiment table;
s33, taking the supernatant of the treated poison-extracted mixed solution in the hair, adding the sample and timing;
and S34, detecting by using an immunofluorescence analyzer and automatically converting into the content of the drugs in the hair sample.
Preferably, in step S33, the hair lysate after treatment in the sampling tube is sucked by a disposable pipette, and 80uL of the hair lysate is added to the sample adding hole of the detection card, and then timing is started.
Preferably, in step S34, the detection card after sample addition is reacted for 5 minutes outside the machine, inserted into an immunofluorescence analyzer, and subjected to a click test, and the display screen of the analyzer is waited to display the detection result and automatically print the detection result.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
the invention designs a method which is simple to operate, short in inspection time, low in cost, free from the limitation of places and sexes in detection, capable of quickly and simply detecting whether an examinee takes drugs or not, suitable for banning, public security policemen and drug rehabilitation departments to carry out preliminary screening and quantitative detection on hairs of suspects with unknown drug abuse, simultaneously suitable for a sanitation and epidemic prevention system, customs detection, preliminary screening of items such as drugs or drug abuse and the like during troop experience, recruit physical examination, government officer physical examination and sensitive department physical examination, and suitable for basic popularization and application.
Drawings
FIG. 1 is a flow chart of a method for detecting common drugs in hair according to the present invention.
Detailed Description
The following describes a specific embodiment of a method for detecting common drugs in hair according to the present invention with reference to fig. 1. The method for detecting common drugs in hair is not limited to the description of the following examples.
Example 1:
this example shows a specific implementation of a method for detecting common drugs in hair, as shown in fig. 1, which includes the following steps:
s1, collecting a hair sample;
s2, hair sample treatment: mixing and grinding a hair sample, a hair lysate and zirconia grinding beads to obtain a mixed solution;
s3, detecting a hair sample: and taking the supernatant of the mixed solution, adding the sample, timing, and detecting to obtain the content of common drugs.
Further, in step S1, when the sample of the hair of the person to be detected is extracted, the worker should wear disposable gloves, and use medical scissors or saw-tooth scissors to closely attach to the surface of the scalp of the person to be extracted to cut the hair with the length of the back portion of the vertex within 3 cm, the hair longer than 3 cm is cut from the hair root end by 3 cm, and if the back portion of the vertex cannot extract enough hair, the head portion closest to the back portion of the vertex is selected.
Further, in step S1, the extracted hair sample is divided into two parts, a and B, each part of the sample is not less than 50 mg in weight, the sample is wrapped by aluminum foil paper, the sample is packaged in a material evidence bag after being respectively filled in the material evidence bag, information such as a sample number, an extraction date and an extractor is filled in the material evidence bag, the seal of the material evidence bag is confirmed by the extractor according to a fingerprint and signed, the extractor refuses to press the fingerprint or sign, the extractor is noted, and the whole extraction process is recorded.
Further, in step S1, the hair extraction worker creates a hair sample extraction information table in which information such as the name of the person to be extracted, the number of the person who is to be extracted, the type of hair to be extracted, the extraction location, the extraction unit, the extraction worker, and the extraction time is described.
Further, in the step S1, the hairs of different persons are respectively extracted, independently packaged and numbered, residues on the extracted equipment in the sampling process are timely cleaned, and cross contamination is prevented.
Further, in step S2, the hair sample processing includes:
weighing 20mg of hair sample to be tested, placing into a 5mL sampling tube, grinding with a portable grinder at 5000rpm for 3 minutes, wherein the 5mL sampling tube contains 2mL of 0.61% tris of 0.5mg/mL proteinase K; 0.85% NACL;1.0% tween-20;0.1% of hair lysate prepared from proclin300 purified water and 95% of zirconia grinding beads having a particle size of 2-5 mm.
Further, in step S3, the hair sample detection includes:
s31, starting the immunofluorescence analyzer, and inserting and reading the ID cards corresponding to the batch of products;
s32, tearing the aluminum foil bag, taking out the detection card, and horizontally placing the detection card on an experiment table;
s33, taking the supernatant of the treated poison-extracted mixed solution in the hair, adding the sample and timing;
and S34, detecting by using an immunofluorescence analyzer and automatically converting into the content of the drugs in the hair sample.
Further, in step S33, the hair lysate after treatment in the sampling tube is sucked by the disposable pipette, and 80uL of the hair lysate is added to the sample addition hole of the detection card, and timing is started.
And further, reacting the detection card after sample adding for 5 minutes outside the card, inserting the detection card into an immunofluorescence analyzer, performing click test, waiting for an instrument display screen to display a detection result, and automatically printing the detection result.
The working principle is as follows: as shown in fig. 1, the hair is surrounded by dense capillary vessels, and drugs in blood enter the hair follicles of the hair from the blood during the growth process of the hair, and the hair containing drug molecules grows out of the surface of the scalp and stably exists for about 3 to 5 days. The hair grows out at a relatively constant rate, and the time, type and dosage of drug use can be presumed. The detection of the abuse drugs in the hair has the unique advantages of easy acquisition and storage of samples, sanitary sampling process, high detectable rate, long detection time, accurate detection result, conjecture of drug absorption of detected personnel within one week to several years and the like. Therefore, the hair test of the drug addict can provide evidence for drug abuse and can also authenticate the type and time of drug intake. The method can be applied to rapid field detection such as screening in entertainment places and major places, and can be used as a basis for drug addiction behavior identification and drug addiction identification and a means for periodic monitoring in community drug rehabilitation and social rehabilitation work.
The reagent adopts the detection principle of a fluorescence immunochromatography competition method, three (morphine, K powder and methamphetamine) synthetic antigens are respectively coated on a detection area on a nitrocellulose membrane, and a goat anti-chicken polyclonal antibody is coated on a contrast area; the sample pad is coated with a mixture according to a certain proportion (a morphine monoclonal antibody and a fluorescent microsphere mark form a compound; a K powder monoclonal antibody and a fluorescent microsphere mark form a compound; an amphetamine monoclonal antibody and a fluorescent microsphere mark form a compound and chicken IGY and a fluorescent microsphere mark form a compound). After the hair sample lysate is dripped into the sample adding hole of the detection card, when the object to be detected contains the antigen substance to be detected, the antigen substance of the species to be detected and the monoclonal antibody for marking the fluorescent microsphere form a complex, and the complex moves along the membrane band due to the capillary chromatography effect, so that the complex cannot be combined with the synthetic antigen coated on the nitrocellulose membrane. And when the substance to be detected is not contained in the substance to be detected, the monoclonal antibody marked with the fluorescent microspheres moves along the membrane strip to be combined with the synthetic antigen coated on the detection line (T line), the concentration of the antigen substance in the sample is in negative correlation with the fluorescent signal, and the concentration value of the sample is calculated by an immunofluorescence analyzer according to the relative fluorescent signal processing value of the sample (T/C: T is the fluorescent signal of the detection line, C is the fluorescent signal of the quality control line) and the calibration curve of the reagent strip (the calibration curve takes T/C as the ordinate and the concentration as the abscissa).
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Claims (9)
1. A method for detecting common drugs in hair is characterized by comprising the following steps:
s1, collecting a hair sample;
s2, hair sample treatment: mixing and grinding a hair sample, a hair lysate and zirconia grinding beads to obtain a mixed solution;
s3, detecting a hair sample: and taking the supernatant of the mixed solution, adding the sample, timing, and detecting to obtain the content of common drugs.
2. The method for detecting common drugs in hair according to claim 1, wherein: in the step S1, when the sample of the hair of the person to be detected is extracted, the worker should wear the disposable gloves, and use the medical scissors or the saw-tooth scissors to closely attach to the surface of the scalp of the person to be extracted to cut the hair with the length of the back of the vertex within 3 cm and the hair longer than 3 cm, cut 3 cm from the hair root end, and select the head part closest to the back of the vertex if the back of the vertex cannot extract enough hair.
3. The method for detecting common drugs in hair according to claim 1, wherein: in the step S1, the extracted hair sample is divided into two parts A and B, each part of the hair sample is not less than 50 mg, the hair sample is wrapped by aluminum foil paper, the hair sample is respectively packaged in material evidence bags, the material evidence bags are packaged, information such as sample numbers, extraction dates and extractors are filled in the material evidence bags, the seal positions of the material evidence bags are confirmed by the extracted personnel by handprints and signature, the extracted personnel refuse to press the handprints or signature, the extractor is noted, and the whole extracting process is recorded.
4. The method for detecting common drugs in hair according to claim 1, wherein: in the step S1, the hair extraction worker creates a hair sample extraction information table, and records information such as the name of the person to be extracted, the number of the person who is to be extracted, the type of the extracted hair, the extraction location, the extraction unit, the extraction worker, and the extraction time.
5. The method for detecting common drugs in hair according to claim 1, wherein: in the step S1, the hairs of different personnel are respectively extracted, independently packaged and uniformly numbered, residues on the extracted equipment in the sampling process are timely cleaned, and cross contamination is prevented.
6. The method for detecting common drugs in hair according to claim 1, wherein: in step S2, the hair sample processing includes:
weighing 20mg of hair sample to be tested, putting the hair sample into a 5mL sampling tube, and grinding the hair sample for 3 minutes by using a portable grinder at the rotating speed of 5000rpm, wherein 2mL of the hair sample contained in the 5mL sampling tube is calculated by 0.5mg/mL proteinase K,0.61 percent; 0.85% of NACL;1.0% tween-20;0.1% of proclin300 purified water and 95% of zirconia grinding beads having a particle size of 2-5 mm.
7. The method for detecting common drugs in hair according to claim 1, wherein: in step S3, the hair sample detection includes:
s31, starting the immunofluorescence analyzer, and inserting and reading the ID cards corresponding to the batch of products;
s32, tearing the aluminum foil bag, taking out the detection card, and horizontally placing the detection card on an experiment table;
s33, taking the supernatant of the treated poison-extracted mixed solution in the hair, adding the sample and timing;
and S34, detecting by using an immunofluorescence analyzer and automatically converting into the content of the drugs in the hair sample.
8. The method for detecting common drugs in hair according to claim 7, wherein: in step S33, the disposable pipette is used to suck the treated hair lysate in the sampling tube, 80uL of the hair lysate is added to the sample adding hole of the detection card, and timing is started.
9. The method for detecting common drugs in hair according to claim 7, wherein: in step S34, the detection card after sample addition is reacted for 5 minutes outside the machine, inserted into an immunofluorescence analyzer, and subjected to a click test, and waits for the display screen of the apparatus to display a detection result and automatically print the detection result.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116296728A (en) * | 2023-02-03 | 2023-06-23 | 中国农业科学院农业质量标准与检测技术研究所 | Method for extracting medicine from hair by dry method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116296728A (en) * | 2023-02-03 | 2023-06-23 | 中国农业科学院农业质量标准与检测技术研究所 | Method for extracting medicine from hair by dry method |
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