CN115160384A - Method for producing galactose and glucose by taking milk as raw material - Google Patents
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- CN115160384A CN115160384A CN202210791307.2A CN202210791307A CN115160384A CN 115160384 A CN115160384 A CN 115160384A CN 202210791307 A CN202210791307 A CN 202210791307A CN 115160384 A CN115160384 A CN 115160384A
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- 229930182830 galactose Natural products 0.000 title claims abstract description 54
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 53
- 239000008103 glucose Substances 0.000 title claims abstract description 53
- 239000008267 milk Substances 0.000 title claims abstract description 48
- 235000013336 milk Nutrition 0.000 title claims abstract description 48
- 210000004080 milk Anatomy 0.000 title claims abstract description 48
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 title claims abstract description 31
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 title claims abstract description 31
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 239000002994 raw material Substances 0.000 title claims abstract description 11
- 238000013375 chromatographic separation Methods 0.000 claims abstract description 17
- 239000008101 lactose Substances 0.000 claims abstract description 16
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 15
- 238000001035 drying Methods 0.000 claims abstract description 14
- 108091005804 Peptidases Proteins 0.000 claims abstract description 12
- 239000004365 Protease Substances 0.000 claims abstract description 12
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 238000010438 heat treatment Methods 0.000 claims abstract description 9
- 239000012043 crude product Substances 0.000 claims abstract description 8
- 239000003513 alkali Substances 0.000 claims abstract description 5
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 5
- 230000002378 acidificating effect Effects 0.000 claims abstract description 4
- 238000006386 neutralization reaction Methods 0.000 claims abstract description 4
- 238000004806 packaging method and process Methods 0.000 claims abstract description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 9
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 7
- 239000000920 calcium hydroxide Substances 0.000 claims description 7
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 7
- 239000000725 suspension Substances 0.000 claims description 7
- 238000001179 sorption measurement Methods 0.000 claims description 6
- 239000002023 wood Substances 0.000 claims description 6
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 5
- 238000005238 degreasing Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 238000003825 pressing Methods 0.000 claims description 4
- 239000008213 purified water Substances 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- 150000001768 cations Chemical class 0.000 claims description 3
- 239000003480 eluent Substances 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 238000004042 decolorization Methods 0.000 claims description 2
- 238000011033 desalting Methods 0.000 claims description 2
- 239000002585 base Substances 0.000 claims 1
- 239000003960 organic solvent Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 238000010612 desalination reaction Methods 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 36
- 239000000047 product Substances 0.000 description 9
- 239000000706 filtrate Substances 0.000 description 4
- 238000005342 ion exchange Methods 0.000 description 4
- 238000002425 crystallisation Methods 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- -1 and simultaneously Substances 0.000 description 2
- 238000005349 anion exchange Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000003957 anion exchange resin Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 208000028659 discharge Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/13—Crystalline forms, e.g. polymorphs
Abstract
The invention discloses a method for producing galactose and glucose by taking milk as a raw material, which comprises the following steps: (1) Using protease to degrease and filter milk to prepare crude lactose liquid; (2) Hydrolyzing the crude lactose liquid under acidic and high-temperature conditions, heating and preserving heat, adding alkali for neutralization, and then filtering; (3) Carrying out decoloration, filtration and desalination treatment on the neutralized solution; (4) Concentrating and carrying out chromatographic separation on the desalted solution to obtain a galactose enrichment solution and a glucose enrichment solution; (5) Concentrating and crystallizing the galactose enrichment solution and the glucose enrichment solution respectively; (6) And respectively centrifuging, drying, crushing and packaging the crystallized galactose and glucose crude products to finally obtain high-quality galactose and glucose with the purity of more than 99%. The method provided by the invention is simple to operate, does not use an organic solvent, and does not have the food safety problem of organic solvent residue.
Description
Technical Field
The invention relates to the technical field of galactose production, in particular to a method for producing galactose and glucose by taking milk as a raw material.
Background
Milk is one of the oldest natural beverages, is known as 'white blood', and has imaginable importance to human bodies. Milk is expressed from female cows as the name implies. Milk is also graded differently in different countries. The milk has complex types, at least more than 100 types, and the main components of the milk comprise water, lactose, fat, phospholipid, protein, inorganic salt and the like. Lactose has low sweetness, is not easy to be absorbed and utilized by adults, is easy to crystallize and causes lactose intolerance if being directly applied to liquid dairy products to replace cane sugar or syrup, and simultaneously, lactose mineral salts, particularly sodium and potassium, have great influence on the taste and the addition of the product. If the lactose liquid is directly discharged, the lactose discharge treatment cost is high and the environmental pollution is serious because the biological oxygen demand is high.
The prior art still stays in the production of a mixture of galactose and glucose by taking milk as a raw material, and no related process for producing single products of galactose and glucose by taking milk as a raw material is found at present.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a method for producing galactose and glucose by taking milk as a raw material, the production method of galactose and the product purification process relate to chromatographic separation and crystallization technologies, the method is advanced, the purity of the produced single galactose and glucose reaches over 99 percent, in addition, the solvent used by the production method is purified water, other organic solvents are not added in the whole process, and the problem of food safety risk caused by organic solvent residue is effectively avoided.
In order to realize the purpose of the invention, the invention is realized by the following technical scheme: a method for producing galactose and glucose from milk comprises the following steps:
(1) Using protease to degrease and filter milk to prepare crude lactose liquid;
(2) Hydrolyzing the crude lactose liquid under acidic and high-temperature conditions, heating and preserving heat, adding alkali for neutralization, and then filtering;
(3) Carrying out decoloration, filtration and desalination treatment on the neutralized solution;
(4) Concentrating and carrying out chromatographic separation on the desalted solution to obtain a galactose enrichment solution and a glucose enrichment solution;
(5) Concentrating and crystallizing the galactose enrichment solution and the glucose enrichment solution respectively;
(6) And respectively centrifuging, drying, crushing and packaging the crystallized galactose and glucose crude products to finally obtain high-quality galactose and glucose with the purity of more than 99%.
The further improvement lies in that: the step (1) comprises the following steps:
a. and (3) heating: adding milk into a reaction kettle, starting stirring, and heating the milk;
b. adding an enzyme: after the temperature of the milk is raised to 60 ℃, adding protease, wherein the adding amount of the protease is 1-5% of the mass of the milk;
c. degreasing: continuously stirring, keeping the temperature of the milk at 50-70 ℃, and degreasing for 1-3 hours;
d. and (3) filtering: and (3) carrying out filter pressing on the degreased milk, wherein the filter pressing equipment is a plate-and-frame filter press.
The further improvement lies in that: the acid in the step (2) is diluted phosphoric acid, the concentration of the diluted phosphoric acid is 5% -40%, and the pH value of the solution after the acid is added is 1-4.
The further improvement lies in that: in the step (2), the high temperature is 90-130 ℃, and the heat preservation time is 2-6 hours.
The further improvement is that: the alkali in the step (2) is calcium hydroxide suspension, the mass fraction of the calcium hydroxide suspension is 10-40%, and the pH value of a solution neutralized by the calcium hydroxide suspension is 3-6.
The further improvement lies in that: the wood activated carbon is adopted for decolorization in the step (3), the addition amount of the wood activated carbon is 0.5 percent of the mass of the reaction solution, the addition temperature is 50-70 ℃, and the heat preservation time is 0.5-2 hours.
The further improvement is that: and (5) concentrating the solution in the step (4), wherein the concentration of the concentrated solution is controlled to be 35-50%.
The further improvement lies in that: six serially connected chromatographic separation columns are adopted in the chromatographic separation in the step (4), the adsorption medium filled in the chromatographic separation columns is sodium type cation adsorption resin, and the used eluent is purified water.
The further improvement lies in that: in the step (5), the galactose enrichment solution is concentrated, and the concentration of the concentrated galactose enrichment solution is 60% -75%, and in the step (5), the glucose enrichment solution is concentrated, and the concentration of the concentrated glucose enrichment solution is 75% -85%.
The further improvement lies in that: the drying temperature in the step (6) is 85-95 ℃, the drying time is 2-5 hours, and the moisture of the semi-lactose and the glucose after drying is controlled below 1.0 percent.
The invention has the beneficial effects that: according to the invention, the protease is adopted to degrease the milk, so that the prepared lactose has high purity and stable crystal form, and is beneficial to subsequent hydrolysis; the invention does not use organic solvent, thus avoiding the pollution of the organic solvent to the product; the invention adopts chromatographic separation and crystallization technology to effectively separate and purify lactose hydrolysate galactose and glucose, and galactose and glucose products with high purity and good quality are obtained.
Drawings
FIG. 1 is a flow chart of the production method of the present invention.
FIG. 2 is a HPLC chart of a mixed solution after milk hydrolysis according to the present invention.
FIG. 3 is an HPLC chart of galactose as a finished product of the present invention.
FIG. 4 is an HPLC chart of the final product glucose of the present invention.
Detailed Description
In order to further understand the present invention, the following detailed description will be made with reference to the following examples, which are only used for explaining the present invention and are not to be construed as limiting the scope of the present invention.
According to fig. 1, 2 and 3, the present embodiment provides a method for producing galactose and glucose from milk, which comprises the following steps:
(1) Using protease to degrease and filter milk to prepare crude lactose liquid;
(2) Hydrolyzing the crude lactose liquid under acidic and high-temperature conditions, heating and preserving heat, adding alkali for neutralization, and then filtering;
(3) Carrying out decoloration, filtration and desalination treatment on the neutralized solution;
(4) Concentrating and carrying out chromatographic separation on the desalted solution to obtain a galactose enrichment solution and a glucose enrichment solution;
(5) Concentrating and crystallizing the galactose enrichment solution and the glucose enrichment solution respectively;
(6) And respectively centrifuging, drying, crushing and packaging the crystallized galactose and glucose crude products to finally obtain high-quality galactose and glucose with the purity of more than 99%.
Examples
Pouring milk into a reaction kettle, starting stirring of the reaction kettle at the rotation speed of 30 revolutions per minute, starting a heating device of the reaction kettle, slowly adding protease into the reaction kettle after the temperature of the milk is raised to 60 ℃, wherein the adding amount of the protease is 3% of the mass of the milk, starting timing after the protease is added, finishing milk degreasing after 2 hours, introducing the milk into a plate-and-frame filter press, collecting filtrate, and carrying out the next operation;
introducing the collected filtrate into a hydrolysis kettle, adding 30 mass percent of diluted phosphoric acid, adjusting the pH value of the solution to 2.1, hydrolyzing at the temperature of 115 ℃ for 4 hours, cooling to 80 ℃, adding 20 mass percent of calcium hydroxide suspension, adjusting the pH value to 4.5, wherein the content of galactose in the hydrolyzed milk is 47.772 percent, the content of glucose is 51.971 percent, filtering, collecting the filtrate, and carrying out the next operation;
wood activated carbon is adopted for decoloring the filtrate in the previous step, the addition amount of the wood activated carbon is 0.5 percent of the mass of the reaction solution, the addition temperature is 62 ℃, the heat preservation time is 1.5 hours, the decoloring effect is determined by testing the light transmittance of the decolored solution, and the light transmittance requirement is more than 92 percent;
enabling the decolored solution to enter an ion exchange column for desalting, enabling two ion exchange columns to be provided, enabling one ion exchange column to be an anode column, filling cation exchange resin in the anode column, enabling one ion exchange column to be an anion exchange column, filling anion exchange resin in the anion exchange column, and testing the conductivity of the desalted solution, wherein the conductivity is required to be 30 mu s/cm smaller;
evaporating and concentrating the desalted solution, wherein the concentration of the concentrated solution is 40%, then separating and removing impurities by using a program type simulated moving bed chromatographic separation device to obtain a galactose enrichment solution and a glucose enrichment solution respectively, wherein the chromatographic separation adopts six chromatographic separation columns and a program control system which are connected in series, an adsorption medium filled in the chromatographic separation columns is sodium type cation adsorption resin, and the used eluent is purified water;
carrying out evaporation concentration on the galactose enrichment solution and the glucose enrichment solution obtained after chromatographic separation respectively, wherein the concentration of the galactose enrichment solution is 65% after concentration, the concentration of the glucose enrichment solution is 82%, and the concentrated galactose enrichment solution and the concentrated glucose enrichment solution are crystallized by adopting crystallization tanks respectively;
and (3) respectively carrying out centrifugal drying on the galactose crude product and the glucose crude product by adopting a centrifugal machine, respectively collecting the dried galactose crude product and the dried glucose crude product, and then drying at the drying temperature of 94 ℃ for 3 hours, wherein the moisture content of the galactose after drying is 0.05 percent, and the moisture content of the glucose is 0.04 percent. The dried product has particles and a small amount of powder, the material is crushed by a crusher and then packaged;
the purity of the packaged galactose product is 99.877%, and the purity of the glucose product is 99717%.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, and such changes and modifications are within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A method for producing galactose and glucose by taking milk as a raw material is characterized by comprising the following steps:
(1) Using protease to degrease and filter milk to prepare crude lactose liquid;
(2) Hydrolyzing the crude lactose liquid under acidic and high-temperature conditions, heating and preserving heat, adding alkali for neutralization, and then filtering;
(3) Carrying out decoloration, filtration and desalting treatment on the neutralized solution;
(4) Concentrating and carrying out chromatographic separation on the desalted solution to obtain a galactose enrichment solution and a glucose enrichment solution;
(5) Concentrating and crystallizing the galactose enrichment solution and the glucose enrichment solution respectively;
(6) And (3) respectively centrifuging, drying, crushing and packaging the crystallized galactose and glucose crude products to finally obtain high-quality galactose and glucose with the purity of more than 99%.
2. The method for producing galactose and glucose by using milk as raw material according to claim 1, wherein the step (1) comprises the following steps:
a. and (3) heating: adding milk into a reaction kettle, starting stirring, and heating the milk;
b. adding an enzyme: after the temperature of the milk is raised to 60 ℃, adding protease, wherein the adding amount of the protease is 1-5% of the mass of the milk;
c. degreasing: continuously stirring, keeping the temperature of the milk at 50-70 ℃, and degreasing for 1-3 hours;
d. and (3) filtering: and (3) carrying out filter pressing on the degreased milk, wherein the filter pressing equipment is a plate-and-frame filter press.
3. The method for producing galactose and glucose from milk according to claim 1, wherein the acid in step (2) is diluted phosphoric acid, the concentration of diluted phosphoric acid is 5% -40%, and the pH of the solution after adding acid is 1-4.
4. The method for producing galactose and glucose from milk according to claim 1, wherein the high temperature in step (2) is 90-130 ℃ and the holding time is 2-6 hours.
5. The method for producing galactose and glucose from milk according to claim 1, wherein the base in step (2) is calcium hydroxide suspension, the mass fraction of the calcium hydroxide suspension is 10% -40%, and the pH value of the solution neutralized by calcium hydroxide suspension is 3-6.
6. The method for producing galactose and glucose by using milk as a raw material according to claim 1, wherein the wood activated carbon is used for decolorization in the step (3), the addition amount of the wood activated carbon is 0.5% of the mass of the reaction solution, the addition temperature is 50-70 ℃, and the heat preservation time is 0.5-2 hours.
7. The method for producing galactose and glucose from milk according to claim 1, wherein the concentration of the solution in step (4) is controlled to be 35% -50%.
8. The method for producing galactose and glucose from milk according to claim 1, wherein the chromatographic separation in step (4) uses six chromatographic separation columns connected in series, the adsorption medium filled in the chromatographic separation columns is sodium type cation adsorption resin, and the eluent used is purified water.
9. The method for producing galactose and glucose by using milk as a raw material according to claim 1, wherein the galactose-enriched liquid is concentrated in step (5) to a concentration of 60% -75% and the glucose-enriched liquid is concentrated in step (5) to a concentration of 75% -85%.
10. The method for producing galactose and glucose from milk according to claim 1, wherein the drying temperature in step (6) is 85-95 ℃, the drying time is 2-5 hours, and the moisture content of galactose and glucose after drying is controlled below 1.0%.
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| Application Number | Priority Date | Filing Date | Title |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210791307.2A CN115160384A (en) | 2022-07-07 | 2022-07-07 | Method for producing galactose and glucose by taking milk as raw material |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116926147A (en) * | 2023-07-19 | 2023-10-24 | 安徽天凯生物科技有限公司 | Method and device for preparing lactose and galacto-oligosaccharide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646847A (en) * | 2019-10-12 | 2021-04-13 | 内蒙古伊利实业集团股份有限公司 | Preparation method of galactose glucose mixture |
| CN112876520A (en) * | 2021-02-07 | 2021-06-01 | 安徽禾庚生物技术有限公司 | Preparation method of high-quality arabinose, galactose, rhamnose and glucuronic acid |
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- 2022-07-07 CN CN202210791307.2A patent/CN115160384A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646847A (en) * | 2019-10-12 | 2021-04-13 | 内蒙古伊利实业集团股份有限公司 | Preparation method of galactose glucose mixture |
| CN112876520A (en) * | 2021-02-07 | 2021-06-01 | 安徽禾庚生物技术有限公司 | Preparation method of high-quality arabinose, galactose, rhamnose and glucuronic acid |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116926147A (en) * | 2023-07-19 | 2023-10-24 | 安徽天凯生物科技有限公司 | Method and device for preparing lactose and galacto-oligosaccharide |
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