CN115155828B

CN115155828B - Pipeline negative pressure threshold value determination and abnormality warning method, controller, system and medium

Info

Publication number: CN115155828B
Application number: CN202210752880.2A
Authority: CN
Inventors: 姚嘉林; 邓温平; 谢澎; 乔德山; 陈键邦; 郭霄亮; 商院芳
Original assignee: Shenzhen Saiqiao Biological Innovation Technology Co Ltd
Current assignee: Shenzhen Saiqiao Biological Innovation Technology Co Ltd
Priority date: 2022-06-29
Filing date: 2022-06-29
Publication date: 2023-08-04
Anticipated expiration: 2042-06-29
Also published as: WO2024001746A1; CN115155828A

Abstract

The invention discloses a method, a device, equipment and a medium for determining a pipeline negative pressure threshold value and warning abnormality, wherein the method comprises the following steps: receiving a pipe installation completion instruction of a target pipeline, and acquiring a normal pressure value of fluid in the target pipeline with an output end communicated with the outside air; executing negative pressure measurement starting operation on the target pipeline; after the pump in the target pipeline is started, if the fluid pressure in the target pipeline meets a preset negative pressure constant condition, acquiring a negative pressure comparison value of the target pipeline; and acquiring a pipe replacement parameter in a pipe installation completion instruction, and determining a negative pressure threshold of the target pipeline according to the pipe replacement parameter, the normal pressure value, the negative pressure comparison value and a preset negative pressure threshold model. According to the invention, after each pipe replacement, the negative pressure threshold value can be accurately determined by automatically combining the external environment and the pipe replacement parameters of the replaced pipe, so that the accuracy of abnormal warning of the cell separation process according to the negative pressure threshold value is ensured, and the smooth and safe cell separation process is ensured.

Description

Pipeline negative pressure threshold value determination and abnormality warning method, controller, system and medium

Technical Field

The invention relates to the technical field of cell separation, in particular to a method, a device, equipment and a medium for determining a pipeline negative pressure threshold value and alarming abnormality.

Background

At present, cell therapy has become an emerging therapeutic means, and a cell separation method plays one of important roles in cell therapy, and the cell separation method often realizes separation of mononuclear cells through a centrifugal cup, and in the process of cell separation, a blood sample needs to be injected into the centrifugal cup and unnecessary liquid components in the centrifugal cup are discharged to a waste liquid bag, so that the inventor finds that blood clots or cell aggregation exists due to different cell activities of blood, and the blood clots or products of the cell aggregation can cause blockage of a blood sample bag and the like, and once the blockage occurs, the cell separation process cannot be normally performed, even safety accidents are caused, and further the blood sample is greatly wasted, and huge damage and loss are caused to patients needing treatment.

In the prior art, in order to avoid the risk of abnormal occurrence of the pipeline, there is a scheme of setting a limit value corresponding to a pipeline related parameter to perform abnormal blockage recognition of cell separation, but since the above various devices and the connecting pipeline thereof are usually disposable products, the pipe needs to be replaced frequently, and the material of the replaced pipe may change (the pipe replacement of various materials may occur in one cell separation machine), therefore, each pipe replacement operation may cause the change of the pipeline related parameter, and meanwhile, the pipeline related parameter may be affected by external environment, so that the accuracy of performing abnormal blockage recognition of the pipeline by setting the pipeline related parameter to a fixed limit value cannot be ensured, which still cannot ensure smooth progress of the cell separation process, and cannot ensure the safety thereof.

Disclosure of Invention

The embodiment of the invention provides a method, a device, equipment and a medium for determining a pipeline negative pressure threshold value and alarming abnormality, which are used for solving the problems that in the prior art, the accuracy of identifying abnormal blockage in a cell separation process cannot be ensured due to the fact that a pipeline related parameter is set to be a fixed limit value.

A method for determining a negative pressure threshold of a cell separation system pipeline comprises the following steps:

receiving a pipe installation completion instruction of a target pipeline, and acquiring a normal pressure value of fluid in the target pipeline, wherein the output end of the normal pressure value is communicated with the outside air;

executing negative pressure measurement starting operation on the target pipeline;

after a pump in the target pipeline is started, if the fluid pressure in the target pipeline meets a preset negative pressure constant condition, acquiring a negative pressure comparison value of the target pipeline;

and acquiring a pipe replacement parameter in the pipe installation completion instruction, and determining a negative pressure threshold of the target pipeline according to the pipe replacement parameter, the normal pressure value, the negative pressure comparison value and a preset negative pressure threshold model.

The cell separation system comprises a target pipeline, a liquid bag communicated with the input end of the target pipeline and a centrifugal cup communicated with the output end of the target pipeline; a first pressure sensor is arranged between the liquid bag and the pump of the target pipeline;

The cell separation system abnormality warning method comprises the following steps:

determining a negative pressure threshold value of a target pipeline in the cell separation system by the method for determining the negative pressure threshold value of the pipeline of the cell separation system;

receiving a cell separation processing instruction, controlling the pump of the target pipeline to start and execute a cell separation operation, and monitoring a first real-time pressure value in a fluid pipeline between the liquid bag and the pump of the target pipeline through the first pressure sensor;

and when the first real-time pressure value is smaller than or equal to the negative pressure threshold value, controlling the pump to be closed, and prompting the abnormal condition of the liquid bag or/and the input end of the target pipeline in a first preset prompting mode.

A controller for executing the above-mentioned cell separation system pipeline negative pressure threshold value determination method, or the above-mentioned cell separation system abnormality warning method.

The cell separation system comprises a target pipeline, a liquid bag communicated with the input end of the target pipeline, a centrifugal cup communicated with the output end of the target pipeline and the controller, wherein a first pressure sensor is arranged between the liquid bag and a pump of the target pipeline; the controller communicates with the first pressure sensor and the pump.

A computer readable storage medium storing a computer program, wherein the computer program when executed by a processor implements the above-mentioned method for determining a negative pressure threshold value of a cell separation system pipeline, or the computer program when executed by a processor implements the above-mentioned method for warning of abnormality of a cell separation system.

The method receives a pipe installation completion instruction of a target pipeline, and obtains a normal pressure value of fluid in the target pipeline, wherein an output end of the normal pressure value is communicated with outside air; executing negative pressure measurement starting operation on the target pipeline; after a pump in the target pipeline is started, if the fluid pressure in the target pipeline meets a preset negative pressure constant condition, acquiring a negative pressure comparison value of the target pipeline; and acquiring a pipe replacement parameter in the pipe installation completion instruction, and determining a negative pressure threshold of the target pipeline according to the pipe replacement parameter, the normal pressure value, the negative pressure comparison value and a preset negative pressure threshold model.

After the target pipeline receives the pipe installation completion instruction, at least part of the pipe in the target pipeline is confirmed to be replaced, at the moment, the negative pressure environment is formed in the target pipeline after the pump in the target pipeline is started by executing negative pressure measurement starting operation on the target pipeline, and then the negative pressure threshold of the target pipeline is automatically determined according to the normal pressure value measured before the pump is started, the negative pressure comparison value obtained when the preset negative pressure constant condition is met after the pump is started, the preset negative pressure threshold model and the pipe replacement parameter corresponding to the replaced pipe. According to the invention, after each pipe replacement operation, external air (external environment influence factors) and pipe replacement parameters of the replaced pipe can be combined automatically, the corresponding negative pressure threshold value can be determined accurately in a targeted manner, the negative pressure threshold value is not required to be set or adjusted manually, the setting efficiency and the accuracy of the negative pressure threshold value are improved, the accuracy of warning of abnormality (such as blockage) of the cell separation process according to the automatically determined negative pressure threshold value is ensured, and the smooth proceeding and the safety of the cell separation process are further ensured.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the description of the embodiments of the present invention will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a flow chart of a method for determining a negative pressure threshold of a cell separation system pipeline according to an embodiment of the present invention;

FIG. 2 is a flowchart of step S30 of a method for determining a negative pressure threshold of a cell separation system according to an embodiment of the present invention;

FIG. 3 is a flow chart of a method for warning of abnormality in a cell separation system according to an embodiment of the invention;

FIG. 4 is a schematic diagram showing the structure of a cell separation system according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of a computer device in accordance with an embodiment of the invention.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

In one embodiment, as shown in fig. 1, a method for determining a negative pressure threshold of a cell separation system pipeline is provided, which includes the following steps:

s10: receiving a pipe installation completion instruction of a target pipeline, and acquiring a normal pressure value of fluid in the target pipeline, wherein the output end of the normal pressure value is communicated with the outside air; the target pipeline comprises a pump and a plurality of pipe sections connected to two ends of the pump, and further the target pipeline can also comprise a pipeline panel for fixedly installing the pipe sections and the pump and the like; the pipe section and the pipe panel of the target pipe need to be replaced frequently (for example, most pipe sections and pipe panels used in the cell separation system belong to disposable consumables and need to be replaced frequently). The materials of the replaced pipe section and the pipe panel in the target pipe may be changed compared with those before replacement, so that the pipe installation completion instruction is generated after the pipe (such as the pipe section or the pipe panel) in the target pipe is replaced and installed, or generated when the pipe of the target pipe is installed for the first time, or generated when the man-machine interface/process flow operation process is manually clicked after the pipe is installed and the negative pressure threshold determination method of the pipe of the cell separation system is involved, for example, after at least part of the pipe in the target pipe is replaced, a preset confirmation button is triggered to generate the pipe installation completion instruction. It is understood that the pipe installation completion command includes pipe replacement parameters corresponding to the replaced pipe, such as material, length, thickness, and other dimensional parameters of the pipe after replacement.

Wherein the process flow is the flow of steps/processes in the cell handling process.

After receiving the pipe installation completion instruction, the output end of the target pipe (such as the end connected with the centrifugal cup in the cell separation system) is controlled to be communicated with the external air, and the inside of the pipe in the target pipe can be in a sterile environment through the sterile air filter in the communicated path. Further, in the present invention, all the pipe sections or pipes are in communication with the outside air, meaning that the pipe sections or pipes are in communication with the outside air through a sterile air filter.

S20: executing negative pressure measurement starting operation on the target pipeline; the purpose of the negative pressure measurement starting operation is as follows: after the negative pressure measurement start operation is performed, if the pump in the target line is started, a negative pressure environment is formed in the target line to further measure a negative pressure limit value that can be reached by the fluid in the target line in the negative pressure environment in a state where the pump is started.

In one embodiment, as shown in fig. 4, the target pipeline 11 includes a first pipe section L1, a pump 111, and a second pipe section L2 connected in sequence; the first pipe section L1 is clamped with a first pressure sensor 15 for measuring the pressure of the fluid in the first pipe section L1; a second pressure sensor 16 for measuring the fluid pressure in the second pipe section L2 is clamped on the second pipe section L2; the output end of the target pipeline 11 is arranged at one end of the second pipe section L2 far away from the pump 111; the input end of the target pipeline 11 is arranged at one end of the first pipe section L1 far away from the pump 111; preferably, the pump 111 in the present invention includes, but is not limited to, a peristaltic pump, and correspondingly, the pump 111 in the present invention may be started by a peristaltic pump in a forward rotation, so as to continuously drive the fluid in the first pipe section L1 to flow toward the second pipe section L2. The first pressure sensor 15 and the second pressure sensor 16 can measure the fluid pressure analog signal corresponding to the point in the first pipe section L1 and the second pipe section L2, respectively, and then the pressure analog signal is converted into an ADC (analog to digital converter, the analog signal is converted into a digital signal) pressure value by AD conversion (analog-to-digital conversion), and the pressure value is transmitted to the controller 12.

It will be appreciated that in the present invention, when the pump, the liquid bag and the pipe section between the pump and the liquid bag of the target pipeline in the cell separation system are replaced (such as material or model replacement), the generation of the pipe installation completion instruction of the target pipeline in the method for determining the negative pressure threshold value of the pipeline of the cell separation system is necessarily triggered.

Further, in the step S10, acquiring a normal pressure value of the fluid in the target pipeline with the output end communicating with the outside air includes: and measuring a fluid pressure value in the second pipe section communicated with the outside air through the second pressure sensor, and determining the measured fluid pressure value as a normal pressure value. That is, in the state that the pump is not started, the output end of the target pipeline (i.e., the end of the second pipe section away from the pump) is communicated with the external air, at this time, the normal pressure value of the fluid in the target pipeline measured by the second pressure sensor located on the second pipe section is the external air pressure, which is generally equal to the atmospheric pressure, and when the external environment changes, the normal pressure value also changes along with the change of the external environment.

Further, step S20, that is, the performing the negative pressure measurement start operation on the target pipeline, includes:

controlling the first pressure sensor to separate from the first pipe segment to release the first pipe segment; that is, in the present invention, the subsequent abnormal warning of the cell separation process performed on the cell separation system according to the negative pressure threshold value mainly occurs in the first tube segment, and therefore, in the present invention, the first pressure sensor clamped on the first tube segment is first controlled to be separated from the first tube segment, that is, the measurement clamping portion corresponding to the first pressure sensor does not clamp the first tube segment any more at this time, and therefore, the first tube segment is free to be released, deformation or the like due to the clamping of the measurement clamping portion is not generated, so that the fluid in the first tube segment which is not deformed can flow more freely, and further, the measurement accuracy will be higher for the whole measurement process of the negative pressure threshold value.

And closing the input end of the target pipeline, and keeping the output end of the target pipeline communicated with the outside air, so that a negative pressure environment is formed in the first pipe section and the second pipe section after the pump is started. It will be appreciated that in this embodiment, the inlet end of the target line (i.e. closed by closure of a first control valve provided at the end of the first pipe section remote from the pump) is closed, wherein the inlet end may be for the inflow of liquid in the liquid bag, or the inflow of liquid into the liquid bag; the output end of the target pipeline (namely, the end of the second pipe section far away from the pump) is communicated with the outside air (the output end of the target pipeline is communicated with the outside air through a sterile air filter), and the liquid at the output end can be used for flowing out the liquid in the target pipeline or flowing back into the target pipeline after being treated. At this time, at the initial time when the pump is not started, the air pressure value in the target pipeline is equal to the outside air pressure; if the pump starts to start continuously (such as a peristaltic pump rotates positively), the gas in the first pipe section is continuously driven to flow towards the second pipe section, at this time, due to the closed input end of the target pipeline, the gas in the first pipe section is less and less, and is continuously in a negative pressure state, meanwhile, due to the fact that the gas driven by the pump to enter the second pipe section from the first pipe section is less and less, the second pipe section is also affected and is continuously in a negative pressure state, so that the whole target pipeline is in a negative pressure environment, and due to the influence of negative pressure in the pipe, the first pipe section and the second pipe section are gradually thinned, and it is required to be stated that the gas pressure value in the first pipe section is lower than the gas pressure value in the second pipe section.

S30: after a pump in the target pipeline is started, if the fluid pressure in the target pipeline meets a preset negative pressure constant condition, acquiring a negative pressure comparison value of the target pipeline; understandably, the fluid pressure in the target line meeting the preset negative pressure constant condition means that: because the whole target pipeline is in a negative pressure environment, the pressure in the corresponding negative pressure environment in the target pipeline is continuously reduced, under the influence of negative pressure in the pipeline, the first pipeline section and the second pipeline section are gradually thinned, when the negative pressure is detected to be reduced to be unchanged or almost unchanged through the second pressure sensor, the negative pressure in the target pipeline reaches a limit value, at the moment, the fluid pressure in the target pipeline is considered to meet a preset negative pressure constant condition, and then the negative pressure gas pressure value which is correspondingly acquired by the second pressure sensor when the preset negative pressure constant condition is met can be acquired, the negative pressure gas pressure value is a negative pressure comparison value, the negative pressure limit which can be achieved in the second pipeline section is reflected, and understandably, the negative pressure limit is also achieved in the first pipeline section after the second pipeline section reaches the negative pressure limit.

S40: and acquiring a pipe replacement parameter in the pipe installation completion instruction, and determining a negative pressure threshold of the target pipeline according to the pipe replacement parameter, the normal pressure value, the negative pressure comparison value and a preset negative pressure threshold model. That is, in this step, the tubing replacement parameter may represent the influence of the material of tubing replacement on the negative pressure threshold (after tubing replacement, the tubing replacement parameter is sent to the controller along with the tubing replacement command and stored in the database or the gas storage area for ready access), the tubing replacement parameter may be obtained by selecting the tubing model on the man-machine interface to determine the corresponding parameter, if the tubing model does not change, the tubing replacement parameter remains unchanged, and the normal pressure value represents the influence of the external environment on the negative pressure threshold, and the negative pressure comparison value is the negative pressure limit that can be reached in the second tube section under the influence of the two, so that, according to the above three (tubing replacement parameter, the normal pressure value, the negative pressure comparison value) and the preset negative pressure threshold model, the negative pressure threshold representing the negative pressure limit in the first tube section may be obtained. It is understood that the negative pressure threshold value is not necessarily a negative pressure gas pressure value directly corresponding to the first pipe section reaching the negative pressure limit, but may be a safety negative pressure threshold value obtained after safety setting is performed according to the negative pressure gas pressure value corresponding to the negative pressure limit.

Understandably, after step S40, further comprising: the pump is turned off, that is, the rotation of the pump is stopped, the first control valve at the input end is turned on, the first pipe section is restored to be communicated with the outside air (or other pipe sections), and the first pressure sensor is clamped on the first pipe section again, so that the first pipe section is located at a measurable position of the first pressure sensor again, and the cell separation treatment is carried out later.

After the target pipeline receives the pipe installation completion instruction, at least part of the pipe in the target pipeline is confirmed to be replaced, at the moment, the negative pressure environment is formed in the target pipeline after the pump in the target pipeline is started by executing negative pressure measurement starting operation on the target pipeline, and then the negative pressure threshold of the target pipeline is automatically determined according to the normal pressure value measured before the pump is started, the negative pressure comparison value obtained when the preset negative pressure constant condition is met after the pump is started, the preset negative pressure threshold model and the pipe replacement parameter corresponding to the replaced pipe. According to the invention, after each pipe replacement operation, external air (external environment influence factors) and pipe replacement parameters of the replaced pipe can be combined automatically, the corresponding negative pressure threshold value can be determined accurately in a targeted manner, the negative pressure threshold value is not required to be set or adjusted manually, the setting efficiency and the accuracy of the negative pressure threshold value are improved, so that the accuracy of abnormal warning of the cell separation process according to the automatically determined negative pressure threshold value is ensured, and the smooth proceeding and the safety of the cell separation process are further ensured.

In an embodiment, as shown in fig. 2, the step S30, that is, after the pump in the target pipeline is started, of obtaining the negative pressure comparison value of the target pipeline if the fluid pressure in the target pipeline meets the preset negative pressure constant condition, includes:

s301, controlling the pump in the target pipeline to start so as to form a negative pressure environment in the first pipe section and the second pipe section; that is, after the pump in the target pipeline is controlled to start (such as a peristaltic pump rotates forward), the gas in the first pipe section is continuously driven to flow towards the second pipe section, at this time, due to the fact that the input end of the closed target pipeline is closed, the gas in the first pipe section is less and less, and is continuously in a negative pressure state, meanwhile, due to the fact that the gas driven by the pump to enter the second pipe section from the first pipe section is less and less, the second pipe section is also affected to be continuously in a negative pressure state, so that the whole target pipeline is in a negative pressure environment, and due to the influence of negative pressure in the pipe, the first pipe section and the second pipe section are gradually thinned, and the air pressure value in the first pipe section is lower than the air pressure value in the second pipe section (namely, the negative pressure air pressure value of the fluid in the negative pressure environment of the second pipe section) needs to be explained.

S302, detecting the negative pressure gas pressure value of the fluid in the negative pressure environment of the second pipe section in real time or at fixed time through the second pressure sensor; that is, since the whole target pipeline is in a negative pressure environment, and the pressure in the corresponding negative pressure environment in the target pipeline is continuously reduced, under the influence of the negative pressure in the pipeline, both the first pipeline section and the second pipeline section are gradually thinned, and at this time (the time interval is set according to the requirement), the negative pressure gas pressure value of the fluid in the negative pressure environment of the second pipeline section is detected by the second pressure sensor at regular time or in real time, so as to determine whether the current fluid in the second pipeline meets the preset negative pressure constant condition according to the negative pressure gas pressure value.

And S303, when the negative pressure gas pressure value meets a preset negative pressure constant condition, closing the pump, and recording the currently measured negative pressure gas pressure value as a negative pressure comparison value, wherein the negative pressure comparison value is smaller than the normal pressure value. That is, when the negative pressure gas pressure value detected by the second pressure sensor drops to be unchanged or almost unchanged, it is indicated that the negative pressure gas pressure value in the target pipeline reaches a limit value at this time, and the subsequent change amplitude of the negative pressure gas pressure value in this state is negligible, at this time, the fluid pressure in the target pipeline is considered to meet the preset negative pressure constant condition, and then the negative pressure gas pressure value acquired correspondingly by the second pressure sensor when the preset negative pressure constant condition is met can be acquired, where the negative pressure gas pressure value is a negative pressure comparison value, and the negative pressure comparison value represents the negative pressure limit that can be reached in the second pipe section. Further, when the negative pressure gas pressure value meets the preset negative pressure constant condition, the pump needs to be turned off at the same time so as to perform subsequent operations.

Further, in the step S303, before the pump is turned off and the currently measured negative pressure gas pressure value is recorded as the negative pressure comparison value, the method further includes:

acquiring all negative pressure gas pressure values in a preset history time before a current time point; that is, in this embodiment, whether or not the negative pressure gas pressure value in a certain period (within a preset history period, which may be set according to the need) before the current time point satisfies the preset negative pressure constant condition is determined by continuously monitoring the negative pressure gas pressure value, and therefore, all the negative pressure gas pressure values acquired within the preset history period before the current time point need to be acquired first.

Determining the difference between the maximum value and the minimum value of all the acquired negative pressure gas pressure values; that is, the difference between the maximum value and the minimum value among all the negative pressure gas pressure values represents the maximum fluctuation range of the negative pressure gas pressure values within the preset history period.

And when the difference value is smaller than a preset fluctuation range, determining that the negative pressure gas pressure value meets a preset negative pressure constant condition. That is, when the maximum fluctuation range of the negative pressure gas pressure value within the preset history period is smaller than the preset fluctuation range, it is indicated that the negative pressure gas pressure value has fallen to be unchanged or almost unchanged, at this time, it is indicated that the negative pressure gas pressure value within the target line reaches the limit value, and the subsequent variation amplitude of the negative pressure gas pressure value in this state will be negligible, at this time, it is considered that the fluid pressure in the target line satisfies the preset negative pressure constant condition.

In an embodiment, in the step S40, the determining the negative pressure threshold of the target pipeline according to the tubing replacement parameter, the normal pressure value, the negative pressure comparison value, and a preset negative pressure threshold model includes:

determining a pressure correlation coefficient between the first pipe section and the second pipe section after the target pipeline is changed according to the pipe changing parameters; that is, the pipe replacement parameter can embody the influence of the material of pipe replacement on the negative pressure threshold value, when the pipe sections with different materials are respectively arranged at the positions of the first pipe section or the second pipe section, different pressure association coefficients between the first pipe section and the second pipe section can be obtained, and then, according to the pressure association coefficients, the negative pressure comparison value corresponding to the second pipe section under the negative pressure limit (when the fluid pressure in the target pipeline meets the preset negative pressure constant condition) can be mapped to the first pipe section, and then the negative pressure limit value corresponding to the first pipe section is obtained. The pressure correlation coefficient may be obtained in advance by performing a pressure test on a different test pipeline (i.e., a pipeline that matches a target pipeline after the pipe is replaced according to the pipe replacement parameter), and the obtained pressure correlation coefficient may be correlated with the pipe replacement parameter to generate a comparison table, and in this step, the pressure correlation coefficient corresponding to the pipe replacement parameter may be searched from the comparison table.

Determining a negative pressure threshold of the target pipeline according to the preset negative pressure threshold model, wherein the preset negative pressure threshold model is as follows:

Y＝k1*k2*(A-B)+B

wherein:

y is the negative pressure threshold; understandably, Y is a negative value.

k1 is a pressure safety coefficient; preferably, k1 may be 0.8 to 1.

k2 is the pressure correlation coefficient; further, k2 may be 0.9-2. Preferably, k1×k2=1.5 or k1×k2=0.9.

A is the negative pressure comparison value; the negative pressure comparison value refers to a negative pressure gas pressure value acquired by the second pressure sensor in the second pipe section when the fluid pressure in the target pipeline meets the preset negative pressure constant condition, and the negative pressure comparison value reflects the negative pressure limit which can be reached in the second pipe section; the negative pressure comparison value A is smaller than the normal pressure value B.

And B is the normal pressure value. The normal pressure value is the ambient air pressure, and is generally equal to the atmospheric pressure, and when the external environment changes, the normal pressure value also changes along with the change of the external environment, and it can be understood that in the embodiment, the setting of the acquiring state and condition of the normal pressure value reflects the consideration of the external environment factors in the process of determining the negative pressure threshold.

The invention also provides an abnormality warning method of the cell separation system, wherein the cell separation system comprises a target pipeline, a liquid bag (such as a blood sample bag) communicated with the input end of the target pipeline and a centrifugal cup communicated with the output end of the target pipeline; a first pressure sensor is arranged between the liquid bag and the pump of the target pipeline; further, a second pressure sensor can be arranged between the centrifugal cup and the pump of the target pipeline; that is, in the present embodiment, the first pressure sensor is not limited to be provided on the first pipe section as long as it is provided on the fluid line between the fluid bag and the pump, and it is only required to be able to measure the first real-time pressure value in the fluid line from the fluid bag to the pump. Similarly, the second pressure sensor may be provided in the fluid line between the centrifugal cup and the pump, and is not limited to be provided in the second pipe section, as long as the second real-time pressure value in the fluid line from the pump to the centrifugal cup can be measured.

Further, as shown in fig. 3, the cell separation system abnormality warning method includes:

s100, determining a negative pressure threshold value of a target pipeline in the cell separation system by the method for determining the negative pressure threshold value of the pipeline of the cell separation system; it is clear that the method for determining the negative pressure threshold value of the pipeline of the cell separation system needs to be performed before the cell separation process is performed on the cell separation system, that is, before the cell separation process is performed, firstly monitoring whether a pipe installation completion instruction of the target pipeline is received, and if the pipe installation completion instruction of the target pipeline is received, determining the negative pressure threshold value of the target pipeline in the cell separation system by the method for determining the negative pressure threshold value of the pipeline of the cell separation system.

As can be appreciated, in the above-described method for determining a negative pressure threshold value of a cell separation system line, closing the input end of the target line means that communication between the first pipe section and the liquid bag is cut off by the first control valve, and the input end of the first pipe section is blocked, and the fluid in the first pipe section can only flow into the second pipe section by the pump. In this embodiment, after the negative pressure threshold is determined in the step S40, the pump is turned off, that is, the rotation of the pump is stopped, the input end of the target pipeline is turned on again, that is, the first pipe section is allowed to resume communication with the liquid bag (the liquid can flow from the liquid bag to the pump through the fluid pipeline, the pump is always rotated during the process of flowing the liquid in the liquid bag into the centrifugal cup), and the first pressure sensor is clamped on the fluid pipeline between the liquid bag and the pump again, so that the fluid pipeline between the liquid bag and the pump is at a measurable position of the first pressure sensor, so that the cell separation process can be performed later; after the operation is completed, a trigger button for presetting a cell separation operation is triggered, and then a cell separation processing instruction is generated, and then the subsequent steps are executed. Understandably, if the pipe installation completion instruction is not received before the cell separation process is required, a trigger button for the preset cell separation operation may be directly triggered, and further, after the cell separation process instruction is generated, the subsequent steps are executed.

S200, receiving a cell separation processing instruction, controlling a pump of the target pipeline to start and execute a cell separation operation, and monitoring a first real-time pressure value in a fluid pipeline between the liquid bag and the pump of the target pipeline through the first pressure sensor; the cell separation operation is set according to a specific operation procedure required for the cell separation process, for example, the cell separation operation may include starting a motor to drive the centrifugal cup to rotate, and the like, which will not be described herein. After receiving a cell separation processing instruction and controlling the pump of the target pipeline to start and execute a cell separation operation, a first real-time pressure value in a fluid pipeline between the liquid bag and the pump of the target pipeline needs to be monitored in real time or at a fixed time (the time interval can be set according to requirements) through a first pressure sensor, and then, whether abnormal warning is needed or not is determined according to the first real-time pressure value.

And S300, when the first real-time pressure value is smaller than or equal to the negative pressure threshold value, controlling the pump to be closed, and prompting the abnormal condition of the liquid bag or/and the input end of the target pipeline in a first preset prompting mode.

When the liquid bag is a blood sample bag and blood in the blood sample bag is not shaken uniformly and a blood clot occurs, and a liquid outlet of the blood sample bag or a pipeline (or/and a pipeline panel) near the liquid outlet is blocked, the pump always rotates in the process of flowing blood in the blood sample bag into the centrifugal cup, at the moment, if the blood in a fluid pipeline between the blood sample bag and the pump cannot flow due to blockage, at the moment, the in-tube pressure of the fluid pipeline between the blood sample bag and the pump gradually becomes smaller, at the moment, the pump continuously rotates to form a negative pressure environment, and when a first real-time pressure value monitored by a first pressure sensor is smaller than or equal to a negative pressure threshold value, the abnormality of the input end of the blood sample bag or/and the target pipeline is confirmed, at the moment, the abnormality of the input end of the blood sample bag or/and the target pipeline can be prompted through a first preset prompting mode, such as displaying an image, a text and the like on a preset display interface, or prompting through playing sound prompting, or prompting through lamplight of different colors or flashing frequencies can be performed; in a specific embodiment, the prompt information may be set as "the blood sample bag or/and the tube internal pressure of the target tube is abnormal" according to the specific situation: negative pressure exceeding "or" blood sample bag or line panel being blocked "and the like. After the abnormal warning is performed, when the cell separation is performed, the whole machine can not run normally (the cell separation operation time is wasted) due to the idle running of the blood sample bag or the input end, but the current cell separation operation can be stopped according to the prompt information, and after the blocking state is released (such as the replacement of a new blood sample bag, the replacement of a blocked pipeline and the like), the execution of the step S100 and the subsequent steps is performed again, so that the occurrence of safety accidents is avoided, and the cell separation efficiency is also improved.

A second pressure sensor is arranged between the centrifugal cup and the pump of the target pipeline; thus, a second real-time pressure value in the fluid line from the pump to the centrifugal cup can be measured by the second pressure sensor. Further, after the pump in the target line is started, the cell separation system abnormality warning method further includes: monitoring, by the second pressure sensor, a second real-time pressure value in the fluid line between the centrifugal cup and the pump of the target line; and acquiring a positive pressure threshold value of a target pipeline in the cell separation system, controlling the pump to be closed when the second real-time pressure value is larger than or equal to the positive pressure threshold value, and prompting the abnormality of the centrifugal cup or/and the output end of the target pipeline in a second preset prompting mode. That is, in this embodiment, the positive pressure threshold value may be obtained first, where the positive pressure threshold value may be determined according to a preset line positive pressure threshold value determination method, or may be preset.

In an embodiment, after the step S10, a preset positive pressure pipeline threshold determining method may be performed to obtain a positive pressure threshold, where the preset positive pressure pipeline threshold determining method includes:

Step one: performing a positive pressure measurement initiation operation on the target line; the purpose of the positive pressure measurement start operation is: after the positive pressure measurement start operation is performed, if the pump in the target line is started, a positive pressure environment is formed in the fluid line between the pump in the target line and the output end, so that a positive pressure limit value which can be reached in a state that the pump is continuously started in the positive pressure environment is further measured by the fluid in the fluid line between the pump and the output end. Understandably, the performing a positive pressure measurement initiation operation on the target pipeline specifically includes: and controlling the output end of the target pipeline communicated with the outside air to be closed, and controlling the input end of the target pipeline to be communicated with the outside air so as to form a positive pressure environment in the second pipe section after the pump is started. It will be appreciated that in this embodiment, the output of the target line is closed (i.e. by closing a second control valve provided at the end of the second pipe section remote from the pump), the input of the target line (i.e. the end of the first pipe section remote from the pump) is communicated with ambient air (the input of the target line is communicated with ambient air via a sterile air filter), at which time the air pressure in the first pipe section of the target line is equalized with the ambient air pressure when the pump is not or is activated. But for the second tube section, at the initial moment when the pump is not started, the air pressure value in the second tube section is equal to the outside air pressure; if the pump starts to start continuously (for example, the peristaltic pump rotates forward), the gas in the first pipe section is continuously driven to flow towards the second pipe section, at this time, because the output end of the target pipeline is closed, the gas in the second pipe section is more and more, and is continuously in a positive pressure state, so that the second pipe section is in a positive pressure environment, and the second pipe section is gradually thickened due to the influence of the positive pressure in the pipe, and it is required to be noted that the gas pressure value in the second pipe section is higher than the gas pressure value in the first pipe section.

Step two: after a pump in the target pipeline is started, collecting a positive pressure gas pressure value of the target pipeline within a preset safety duration and collecting time of the positive pressure gas pressure value; the preset safety time period can be set according to the sustainable blocking time period for which the non-fried cup can be adhered after the centrifugal cup in the cell separation system is blocked (for example, the preset safety time period is set to be 0.7-0.9 times of the sustainable blocking time period), so that the safety of determining the positive pressure threshold value through the pipeline positive pressure threshold value determining method is ensured. Further, the second step specifically comprises: controlling pump activation in the target line such that a positive pressure environment is established within the second pipe segment; and detecting the positive pressure gas pressure value of the fluid in the positive pressure environment of the second pipe section in real time or at fixed time through the second pressure sensor. That is, since the second pipe section is in a positive pressure environment, and the pressure in the positive pressure environment continuously increases, the second pipe section gradually thickens under the influence of the positive pressure in the pipe, and at this time, the positive pressure gas pressure value of the fluid in the positive pressure environment of the second pipe section and the corresponding acquisition time thereof are detected by the second pressure sensor at a timing (time interval is set according to the requirement) or in real time. It should be noted that the positive pressure gas pressure value in the second pipe section will be higher than the gas pressure value (equal to the outside air pressure) in the first pipe section. And after the pump is started for a preset safety duration, the pump is closed, and all the positive pressure gas pressure values acquired in the preset safety duration and the acquisition time corresponding to the positive pressure gas pressure values are recorded. That is, after the pump is started for a preset safety period, the centrifugal cup in the cell separation system may have a risk of frying, so that the pump needs to be turned off at this time, and then the positive pressure threshold value is further determined according to all the positive pressure gas pressure values collected in the recorded preset safety period and the collection time corresponding to the positive pressure gas pressure values, so that the accuracy of the determined positive pressure threshold value is improved while the safety of the positive pressure threshold value is ensured by the pipeline positive pressure threshold value determination method.

Step three: and acquiring a pipe replacement parameter in the pipe installation completion instruction, and determining a positive pressure threshold of the target pipeline according to the pipe replacement parameter, the positive pressure gas pressure value, the acquisition time and the normal pressure value. That is, in this step, the pipe replacement parameter may represent the influence of the material of the pipe replacement on the positive pressure threshold, while the normal pressure value represents the influence of the external environment on the positive pressure threshold, and the positive pressure gas pressure value and the collection time may represent the positive pressure variation trend that may actually occur in the second pipe section under the influence of the two, so that the positive pressure variation trend when the output end of the second pipe section is blocked and is in the positive pressure environment can be determined according to the four parameters (the pipe replacement parameter, the normal pressure value, the positive pressure gas pressure value and the collection time), and then the positive pressure variation limit value corresponding to the variation trend is determined according to the variation trend, and then the positive pressure threshold is determined according to the positive pressure variation limit value.

In one embodiment, the third step includes:

firstly, recording a difference value between each positive pressure gas pressure value and the normal pressure value acquired in the preset safety duration as a gas pressure difference, and associating each gas pressure difference with each acquisition time in a one-to-one correspondence manner; constructing a time-air pressure curve according to all the air pressure differences and the corresponding acquisition time; that is, a time-air pressure rectangular coordinate system is established first, and then a time-air pressure curve is established in the time-air pressure rectangular coordinate system according to air pressure differences corresponding to different acquisition times in all the preset safety time periods. Further, before constructing the time-air pressure curve, data screening processing is performed on all the air pressure differences to obtain interference data; the interference data comprise the air pressure difference exceeding a preset air pressure jumping range (set according to requirements) and the corresponding acquisition time; after deleting the interference data, constructing a time-air pressure curve according to all the residual air pressure differences and the corresponding acquisition time. That is, the accuracy of the resulting time-air pressure curve can be improved by removing the air pressure value at which the data is jumped to the excessive pressure.

Secondly, acquiring parameter attribute values corresponding to a preset type of linear parameter in the time-air pressure curve; that is, after the time-air pressure curve is constructed, attribute values corresponding to one or more preset type linear parameters (such as slope, rising trend, etc.) can be extracted therefrom, and all sets of attribute values corresponding to the preset type linear parameters are the parameter attribute values.

Thirdly, a preset parameter-threshold value comparison table corresponding to the preset type linear parameter and the pipe replacement parameter is called, wherein the preset parameter-threshold value comparison table comprises a plurality of parameter value sets, and each parameter value set corresponds to one pressure limit value; that is, each preset parameter-threshold value comparison table corresponds to a set of linear parameter types, and each preset parameter-threshold value comparison table includes a plurality of parameter value sets corresponding to the set of linear parameter types, and each parameter value set is a parameter value (equivalent to the attribute value) corresponding to each linear parameter in the set of linear parameter types one by one. Meanwhile, the pressure limit value corresponding to each parameter value group is obtained by positive pressure test according to a target pipeline corresponding to a group of pipe parameters (corresponding to the pipe replacement parameters), specifically, when different test pipelines (namely pipelines consistent with the target pipeline after pipe replacement according to the pipe parameters) are subjected to positive pressure test, when pipe sections of different materials are respectively arranged at the positions of the first pipe section or the second pipe section, different parameter value groups and corresponding pressure limit values can be obtained, the parameter value groups and the pressure limit values obtained through the positive pressure test are correspondingly related to the pipe parameters, and then, a preset parameter-threshold value comparison table can be generated and stored according to the related parameter value groups, the pressure limit values, the pipe parameters and the linear parameter types for being adjusted at any time. In this embodiment, the preset parameter-threshold value comparison table corresponding to the preset type of linear parameter and the pipe replacement parameter is: the linear parameter type of the preset parameter-threshold value comparison table is matched with the linear parameter of the preset type, and the pipe parameter of the preset parameter-threshold value comparison table is matched with the pipe replacement parameter. Wherein, matching means consistent or fluctuating within a certain range.

Then, determining the parameter value group matched with the parameter attribute value from the preset parameter-threshold value comparison table; in this step, after the preset parameter-threshold value comparison table corresponding to the preset type of linear parameter and the pipe replacement parameter is called, a parameter value group that is consistent with the parameter attribute value or fluctuates within a preset range may be queried from the comparison table.

And finally, determining the positive pressure threshold value of the target pipeline according to the pressure limit value corresponding to the parameter value group matched with the parameter attribute value. That is, after the parameter value group matching the parameter attribute value is determined, the pressure limit value associated with the determined parameter value group in the preset parameter-threshold value comparison table is determined as the positive pressure limit value that can be finally reached by the pressure change trend corresponding to the time-air pressure curve, and further, according to the positive pressure limit value, the positive pressure threshold value of the target pipeline may be determined, for example, the positive pressure threshold value may be set to a certain proportion, for example, between 0.8 and 1, of the positive pressure limit value.

In another embodiment, after associating each of the air pressure differences with each of the acquisition times in a one-to-one correspondence, the third step further includes:

Inputting the pipe replacement parameters, all the air pressure differences and the corresponding acquisition time into a preset positive pressure threshold prediction model to obtain a predicted pressure threshold output by the preset positive pressure threshold prediction model; the preset positive pressure threshold prediction model is constructed based on a neural network; that is, in this embodiment, the preset positive pressure threshold prediction model may be obtained by training based on a neural network model and through historical positive pressure test data (the historical positive pressure test data includes a historical trend change curve and a corresponding historical positive pressure limit value thereof, and each historical trend change curve corresponds to a set of pipeline parameters), through the preset positive pressure threshold prediction model, the input change trend characteristics of the air pressure difference along with the collection time may be extracted, and meanwhile, the preset positive pressure threshold prediction model may also determine all pipeline parameters corresponding to the air pressure difference according to the pipe replacement parameters, further obtain a historical trend change curve according to the determined pipeline parameters, further match the extracted change trend characteristics with the obtained historical trend change curve, and when the matching is successful, output the historical positive pressure limit value corresponding to the successfully matched historical trend change curve as the predicted pressure threshold.

And determining a positive pressure threshold of the target pipeline according to the predicted pressure threshold. That is, in this embodiment, the predicted pressure threshold corresponds to a positive pressure limit value that can be eventually reached by the pressure change trend corresponding to the present line replacement, and therefore, according to this predicted pressure threshold, the positive pressure threshold of the target line may be determined, for example, the positive pressure threshold may be set to a certain proportion of the predicted pressure threshold, for example, between 0.8 and 1.

Understandably, after step three, further comprising: and closing the pump, namely stopping the rotation of the pump, opening a second control valve at the output end, and enabling the second pipe section to be communicated with the outside air (or other pipe sections) so as to facilitate the subsequent cell separation treatment.

According to the embodiment of the invention, after each pipe replacement operation, external air (external environment influence factors) and pipe replacement parameters of the replaced pipe can be combined automatically, the positive pressure threshold corresponding to the external air (external environment influence factors) can be determined accurately in a targeted manner, the positive pressure threshold is not required to be set or adjusted manually, the setting efficiency and the accuracy of the positive pressure threshold are improved, the accuracy of warning of abnormality (such as blockage) of the cell separation process according to the automatically determined positive pressure threshold is ensured, and the smooth proceeding and the safety of the cell separation process are further ensured.

As will be appreciated, in the above examples, when the blood in the blood sample bag flows to the centrifuge cup or the centrifuge cup is filled with the cell separation liquid during centrifugation, a blockage occurs in the liquid inlet channel of the centrifuge cup or the liquid inlet is blocked during centrifugation of the centrifuge cup, since the pump continues to rotate, the blood drawn from the blood sample bag and entering the second tube section will not flow into the centrifuge cup, but the pump continues to rotate, the pump continues to draw the blood in the blood sample bag into the second tube section, therefore, the liquid tube between the pump and the centrifuge cup (to which the blockage corresponding to the waste liquid bag is fed) gradually thickens due to the gradual increase in the pressure in the tube, i.e. there is a tendency of positive pressure and rising, when the second real-time pressure value measured by the second pressure sensor is greater than or equal to the threshold value, an abnormality is confirmed in the output end of the centrifuge cup or/and the target tube (blockage of the centrifuge cup may cause the positive pressure cup, thereby rendering the whole centrifuge cup unusable and the blood sample contaminated); the blockage of the centrifugal cup and the waste liquid bag belongs to the problem of abnormal blockage of the centrifugal cup or/and the output end of the target pipeline, at the moment, the centrifugal cup or/and the output end of the target pipeline can be prompted to be abnormal through a second preset prompt mode (the second preset prompt mode can be the same as or different from the first preset prompt mode), such as displaying prompts of images, characters and the like on a preset display interface or playing sound prompts, the prompt can be carried out by lamplight with different colors or flashing frequencies; in a specific embodiment, the prompt information may be set as "centrifugal cup or/and abnormal tube internal pressure at the output end of the target tube" according to specific situations: negative pressure exceeding standard or liquid inlet blockage of centrifugal cup, etc. After the abnormal warning is carried out, when the cell separation is carried out, the current cell separation operation can be stopped according to the prompt information, the problems that the centrifugal cup is fried, the blood sample is polluted or the next operation cannot be carried out due to the blockage of the centrifugal cup, the waste liquid bag or the output end are solved, meanwhile, after the blockage state (such as new pipeline replacement and the like) is removed according to the received prompt information, the step S100 and the subsequent steps are carried out again, so that the occurrence of safety accidents is avoided, and the cell separation efficiency is improved.

It will be appreciated that in the above example, both the blood sample bag and the centrifuge cup are blocked during flow into the centrifuge cup, at which point the fluid conduit between the blood sample bag and the pump tapers as the pressure in the tube gradually decreases as the pump continues to rotate forward, i.e. the first real-time pressure value measured by the first pressure sensor has a tendency to negative pressure and to drop. The fluid pipeline between the pump and the centrifugal cup gradually thickens due to gradual increase of the pressure in the tube, namely, the second real-time pressure value measured by the second pressure sensor has positive pressure and rising trend, under the condition that the pump continues to rotate, if the first real-time pressure value measured by the first pressure sensor is smaller than or equal to a negative pressure threshold value, and the second real-time pressure value measured by the second pressure sensor is larger than or equal to the positive pressure threshold value, the blood sample bag and the centrifugal cup are considered to be abnormal, at the moment, the blood sample bag and the centrifugal cup can be prompted through a first preset mode or/and a second preset prompt mode, and the prompt information can be "the pressure in the tube is abnormal: positive/negative pressure exceeding standard or blockage of blood sample bag, blockage of centrifugal cup, etc.

In the above embodiment of the present invention, the negative pressure threshold and/or positive pressure threshold of the target pipeline in the cell separation system may be determined first, and then, after each pipe replacement operation, the negative pressure threshold and/or positive pressure threshold corresponding to the external air (external environmental influencing factor) and the pipe replacement parameter of the replaced pipe may be determined accurately in a targeted manner, without manually setting or adjusting the negative pressure threshold and/or positive pressure threshold, so that the setting efficiency and accuracy of the negative pressure threshold and/or positive pressure threshold are improved, and thus, the accuracy of abnormal warning of the cell separation process according to the automatically determined negative pressure threshold and/or positive pressure threshold is ensured, and further, the smooth proceeding and safety of the cell separation process is ensured.

It should be understood that the sequence number of each step in the foregoing embodiment does not mean that the execution sequence of each process should be determined by the function and the internal logic, and should not limit the implementation process of the embodiment of the present invention.

In one embodiment, a device for determining a negative pressure threshold value of a cell separation system pipeline is provided, and the device for determining the negative pressure threshold value of the cell separation system pipeline is in one-to-one correspondence with the method for determining the negative pressure threshold value of the cell separation system pipeline in the embodiment. The cell separation system pipeline negative pressure threshold value determining device comprises:

the receiving module is used for receiving a pipe installation completion instruction of the target pipeline and acquiring a normal pressure value of fluid in the target pipeline, wherein the output end of the normal pressure value is communicated with the outside air;

an execution module that performs a negative pressure measurement start operation on the target line;

the acquisition module is used for acquiring a negative pressure comparison value of the target pipeline if the fluid pressure in the target pipeline meets a preset negative pressure constant condition after the pump in the target pipeline is started;

the determining module is used for determining the negative pressure threshold of the target pipeline according to the pipe replacement parameter, the normal pressure value, the negative pressure comparison value and a preset negative pressure threshold model due to the fact that the pipe replacement parameter in the pipe installation completion instruction is obtained.

For the specific definition of the cell separation system line negative pressure threshold value determining device, reference may be made to the definition of the cell separation system line negative pressure threshold value determining method hereinabove, and the detailed description thereof will be omitted. All or part of each module in the cell separation system pipeline negative pressure threshold value determining device can be realized by software, hardware and a combination thereof. The above modules may be embedded in hardware or may be independent of a processor in the computer device, or may be stored in software in a memory in the computer device, so that the processor may call and execute operations corresponding to the above modules.

In one embodiment, a cell separation system abnormality warning device is provided, which corresponds to the cell separation system abnormality warning method in the above embodiment one by one. This cell separation system abnormality warning device includes:

a determining unit for determining a negative pressure threshold value of a target pipeline in the cell separation system by the negative pressure threshold value determining method of the pipeline of the cell separation system;

a control unit for controlling the pump of the target pipeline to start and perform cell separation operation due to receiving a cell separation processing instruction, and monitoring a first real-time pressure value in a fluid pipeline between the liquid bag and the pump of the target pipeline through the first pressure sensor;

And the prompting unit is used for controlling the pump to be closed when the first real-time pressure value is smaller than or equal to the negative pressure threshold value and prompting the abnormal condition of the liquid bag or/and the input end of the target pipeline in a first preset prompting mode.

For specific limitations of the cell separation system abnormality warning device, reference may be made to the above limitations of the cell separation system abnormality warning method, and no further description is given here. All or part of the modules in the abnormality warning device of the cell separation system can be realized by software, hardware and a combination thereof. The above modules may be embedded in hardware or may be independent of a processor in the computer device, or may be stored in software in a memory in the computer device, so that the processor may call and execute operations corresponding to the above modules.

The invention also provides a controller for executing the method for determining the negative pressure threshold value of the cell separation system pipeline or the method for warning the abnormality of the cell separation system. For specific limitations of the controller, reference may be made to the above-mentioned limitation of the method for determining the negative pressure threshold value of the pipeline of the cell separation system and the method for warning of abnormality of the cell separation system, which are not described herein. The various modules in the controller described above may be implemented in whole or in part by software, hardware, and combinations thereof. The above modules may be embedded in hardware or may be independent of a processor in the computer device, or may be stored in software in a memory in the computer device, so that the processor may call and execute operations corresponding to the above modules. It will be appreciated that the controller may be considered one or more computer devices including a processor, memory, network interface, and database connected by a system bus, as shown in fig. 5. Wherein the processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system, computer programs, and a database. The internal memory provides an environment for the operation of the operating system and computer programs in the non-volatile storage media. The database of the computer device is used for storing data used in the method for determining the negative pressure threshold value of the pipeline of the cell separation system or the method for warning the abnormality of the cell separation system in the above embodiment. The network interface of the computer device is used for communicating with an external terminal through a network connection. The computer program when executed by the processor is used for realizing a cell separation system pipeline negative pressure threshold value determining method or a cell separation system abnormality warning method.

As shown in fig. 4, the present invention further provides a cell separation system, which includes a target pipeline 11, a liquid bag 13 connected to an input end of the target pipeline 11, a centrifugal cup 14 connected to an output end of the target pipeline 11, and the controller 12, wherein a first pressure sensor 15 is disposed between the liquid bag 13 and a pump 111 of the target pipeline 11; the controller 12 communicates with the first pressure sensor 15 and the pump 111. Further, a second pressure sensor 16 is provided between the centrifugal cup 14 and the pump 111 of the target pipeline 11; a second real-time pressure value in the fluid line from the pump 111 to the centrifugal cup 14 can be measured by means of a second pressure sensor 16. The controller 12 is also in communication with the second pressure sensor 16. For more specific limitations on the cell separation system and the controller, reference may be made to the above-mentioned limitation on the method for determining the negative pressure threshold value of the pipeline of the cell separation system and the above-mentioned method for warning of abnormality of the cell separation system, which are not described in detail herein.

In one embodiment, a computer readable storage medium is provided, where a computer program is stored, where the computer program when executed by a processor implements the above method for determining a negative pressure threshold value of a line of a cell separation system, or where the computer program when executed by a processor implements the above method for warning of abnormality of a cell separation system.

Those skilled in the art will appreciate that implementing all or part of the above described methods may be accomplished by way of a computer program stored on a non-transitory computer readable storage medium, which when executed, may comprise the steps of the embodiments of the methods described above. Any reference to memory, storage, database, or other medium used in the various embodiments provided herein may include non-volatile and/or volatile memory. The nonvolatile memory can include Read Only Memory (ROM), programmable ROM (PROM), electrically Programmable ROM (EPROM), electrically Erasable Programmable ROM (EEPROM), or flash memory. Volatile memory can include Random Access Memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in a variety of forms such as Static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double Data Rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous Link DRAM (SLDRAM), memory bus direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), among others.

It will be apparent to those skilled in the art that, for convenience and brevity of description, only the above-described division of the functional units and modules is illustrated, and in practical application, the above-described functional distribution may be performed by different functional units and modules according to needs, i.e. the internal structure of the apparatus is divided into different functional units or modules to perform all or part of the above-described functions.

The above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention, and are intended to be included in the scope of the present invention.

Claims

1. A method for determining a negative pressure threshold of a cell separation system pipeline, comprising:

after a pump in the target pipeline is started, if the fluid pressure in the target pipeline meets a preset negative pressure constant condition, acquiring a negative pressure comparison value of the target pipeline; the negative pressure comparison value refers to a negative pressure gas pressure value which is correspondingly acquired when the target pipeline meets the preset negative pressure constant condition;

2. The method for determining a negative pressure threshold value of a cell separation system line according to claim 1, wherein the target line comprises a first pipe section, a pump, and a second pipe section connected in sequence; a first pressure sensor for measuring the pressure of the fluid in the first pipe section is clamped on the first pipe section; a second pressure sensor for measuring the fluid pressure in the second pipe section is clamped on the second pipe section; the output end of the target pipeline is arranged at one end of the second pipe section far away from the pump; the input end of the target pipeline is arranged at one end of the first pipe section far away from the pump;

The executing the negative pressure measurement starting operation on the target pipeline comprises the following steps:

controlling the first pressure sensor to separate from the first pipe segment to release the first pipe segment;

and closing the input end of the target pipeline, and keeping the output end of the target pipeline communicated with the outside air, so that a negative pressure environment is formed in the first pipe section and the second pipe section after the pump is started.

3. The method for determining a negative pressure threshold value of a cell separation system according to claim 2, wherein the acquiring the normal pressure value of the fluid in the target line, the output end of which is in communication with the outside air, comprises:

and measuring a fluid pressure value in the second pipe section communicated with the outside air through the second pressure sensor, and determining the measured fluid pressure value as a normal pressure value.

4. The method for determining a negative pressure threshold value of a cell separation system according to claim 2, wherein the obtaining the negative pressure comparison value of the target line if the fluid pressure in the target line satisfies a preset negative pressure constant condition after the pump in the target line is started, comprises:

controlling the pump in the target pipeline to start so as to form a negative pressure environment in the first pipe section and the second pipe section;

Detecting a negative pressure gas pressure value of fluid in a negative pressure environment of the second pipe section in real time or at fixed time through the second pressure sensor;

and when the negative pressure gas pressure value meets a preset negative pressure constant condition, closing the pump, and recording the currently measured negative pressure gas pressure value as a negative pressure comparison value, wherein the negative pressure comparison value is smaller than the normal pressure value.

5. The method for determining a negative pressure threshold value of a cell separation system line according to claim 4, wherein before turning off the pump and recording a currently measured negative pressure gas pressure value as a negative pressure comparison value, further comprising:

acquiring all negative pressure gas pressure values in a preset history time before a current time point;

determining the difference between the maximum value and the minimum value of all the acquired negative pressure gas pressure values;

and when the difference value is smaller than a preset fluctuation range, determining that the negative pressure gas pressure value meets a preset negative pressure constant condition.

6. The method for determining a negative pressure threshold of a cell separation system line according to claim 1, wherein the determining the negative pressure threshold of the target line according to the tubing replacement parameter, the normal pressure value, the negative pressure comparison value, and a preset negative pressure threshold model includes:

Determining a pressure correlation coefficient between the first pipe section and the second pipe section after the target pipeline is changed according to the pipe changing parameters;

Y＝k1*k2*(A-B)+B

wherein:

y is the negative pressure threshold;

k1 is a pressure safety coefficient;

k2 is the pressure correlation coefficient;

a is the negative pressure comparison value;

and B is the normal pressure value.

7. The cell separation system abnormality warning method is characterized in that the cell separation system comprises a target pipeline, a liquid bag communicated with the input end of the target pipeline and a centrifugal cup communicated with the output end of the target pipeline; a first pressure sensor is arranged between the liquid bag and the pump of the target pipeline;

determining a negative pressure threshold value of a target line in the cell separation system by the cell separation system line negative pressure threshold value determining method according to any one of claims 1 to 6;

8. A controller for performing the cell separation system line negative pressure threshold determination method according to any one of claims 1 to 6, or the cell separation system abnormality warning method according to claim 7.

9. A cell separation system, comprising a target pipeline, a liquid bag communicated with an input end of the target pipeline, a centrifugal cup communicated with an output end of the target pipeline, and the controller of claim 8, wherein a first pressure sensor is arranged between the liquid bag and a pump of the target pipeline; the controller communicates with the first pressure sensor and the pump.

10. A computer-readable storage medium storing a computer program, wherein the computer program when executed by a processor implements the line negative pressure threshold value determination method according to any one of claims 1 to 6, or the computer program when executed by a processor implements the cell separation system abnormality warning method according to claim 7.