CN115120186A

CN115120186A - Subcutaneous detection device, system and method based on conical mirror structure

Info

Publication number: CN115120186A
Application number: CN202110336889.0A
Authority: CN
Inventors: 不公告发明人
Original assignee: Shanghai Jinguan Technology Co ltd
Current assignee: Shanghai Jinguan Technology Co ltd
Priority date: 2021-03-29
Filing date: 2021-03-29
Publication date: 2022-09-30

Abstract

The invention provides a subcutaneous detection device based on a conical mirror structure, which comprises an excitation light source component, a conical mirror structure, a first imaging component, a second imaging component and a detector, wherein the excitation light source component is used for emitting excitation light, and the excitation light emits signal light after being emitted or refracted by a subcutaneous biomarker; the light emitted from the convex surface of the conical mirror structure is an annular light beam; the annular light beam forms an annular excitation area through the first light path. The possible risk of excitation of the point-like light spots is avoided, the signal collection efficiency is improved, and the safety is high; simple structure, with low costs, can adjust the degree of depth of surveying the position through adjusting the conical mirror structure, flexible operation. The detection system and the detection method have corresponding advantages due to the adoption of the subcutaneous detection device based on the conical mirror structure, have simple and efficient steps, can acquire the biological characteristic information of the same subcutaneous depth level according to the requirement, and are favorable for further popularization and application of the subcutaneous noninvasive detection technology.

Description

Subcutaneous detection device, system and method based on conical mirror structure

Technical Field

The invention belongs to the technical field of non-invasive detection, and particularly relates to a subcutaneous detection device based on a conical lens structure, a corresponding detection system and a detection method.

Background

This section is intended to provide a background or context to the embodiments of the invention that are recited in the claims. The description herein is not admitted to be prior art by inclusion in this section.

The application of the non-invasive detection technology has been popularized in various fields in life, and particularly has a universal application in medical examination related to human health and further needs to be further deepened and popularized. The biological characteristics can be reflected through the characteristics of the optical information, and treatment and daily health monitoring can be powerfully supported after analysis.

The Raman spectrum detection technology can reflect the change of human tissue cell molecules in the application of the biomedical field, and is a new technology for early-stage lesion detection. The method has the characteristics of no pain, no wound, simplicity, rapidness and the like, can solve the problems of the conventional detection method, and is one of potential methods for applying the non-invasive biochemical analysis of blood. Different Raman peaks are the characteristics of certain specific molecules, so that the Raman spectrum has the functions of qualitative analysis and distinguishing similar substances, the peak intensity of the Raman spectrum is in direct proportion to the concentration of the corresponding molecules, and the Raman spectrum can also be used for quantitative analysis and can provide a theoretical basis for clinical diagnosis. Whether the blood sample is affected by the disease can be judged according to the characteristic peak intensity of the blood sample in the Raman spectrum in the future.

Taking a raman detection system as an example, the existing non-invasive detection system generally includes a laser light source, a light path component, and a detection component. The laser light source is used as a laser for exciting Raman signal light, and the laser light is focused by a lens of the excitation light path component and then irradiates a tested sample. The excitation light path component filters and focuses the excitation light; the Raman signal light is collected, filtered and transmitted to a detection component, and the Raman signal intensity at different wavelengths is detected. The excitation light is focused into a point to irradiate the surface of the sample, and the generated Raman signal light radiates to the periphery by taking the excitation light irradiation point as the center.

Therefore, how to effectively and sufficiently collect signals in the non-invasive detection is a prominent problem of optical path design in the non-invasive detection system and one of the key designs to be optimized in the current technology. On the other hand, the problem before collection is how to accurately focus the excitation light to the location to be detected, which concerns the complexity of sample preparation and whether it can be deployed outside the laboratory.

Of which the application in subcutaneous biomarker detection is particularly prominent. The skin, the organ with the largest surface area and the most useful, accounts for approximately eight percent of the total weight of the human body, contains 25 to 30 percent of the total circulating blood of the human body, and consists of epidermis, dermis and subcutaneous fat. Interstitial fluid or blood under the skinContains a plurality of biospecific markers which are closely related with the health condition and the disease degree of the human body. However, many of the current medical techniques are difficult to detect the biomarkers through the skin in a non-invasive manner, for example, blood glucose detection requires blood drawing tests or finger-prick blood drawing tests. The non-invasive detection technology is of great significance to subcutaneous medical detection, and especially if ordinary people can monitor personal health in places other than a medical laboratory, the application of the non-invasive detection technology is indispensable. For example, in the application of the conventional raman spectroscopy, which can only test the depth of hundreds of micrometers below the surface, and can detect the spectral information of deep subcutaneous biomarkers without damage, as shown in fig. 1, the excitation light can generate raman signal light at different tissue depths (skin a, subcutaneous tissue B, blood vessel U) in the excitation area and around the excitation area, and according to the photon migration theory, the offset distance Δ S from the central excitation point along the spatial offset direction X is the same as that of the excitation area ₁ The larger the signal light from the deeper sample is. Therefore, there is a need to develop a subcutaneous detection device, a subcutaneous detection system and a corresponding detection method based on a conical mirror structure, which can effectively detect a specific subcutaneous depth part, are beneficial to improving the collection efficiency and have reliable detection results.

The above information disclosed in the background section is only for enhancement of understanding of the background of the present disclosure and therefore it may contain information that does not constitute prior art that is known to a person of ordinary skill in the art. The statements in this background section merely indicate prior art as known to the public and are not, of course, representative of existing art in this field.

Disclosure of Invention

The invention aims to solve all or part of problems in the prior art, and provides a subcutaneous detection device and system based on a conical mirror structure and a corresponding detection method.

The following description is made of some principles and concepts which may be related to the invention and are intended to be illustrative or schematic in nature and not restrictive in character, and is not intended to limit the scope of the invention.

The invention is based on the principle of the SORS technologyThe fundamental starting point is photon migration theory, as shown in fig. 1, when excitation light is incident to the surface layer of a sample to be measured, the surface layer sample is excited or scattered to generate broadband fluorescence, wherein a part of scattered light reaches the inside of the sample, raman scattered photons generated at the deep layer inside the sample are easier to migrate transversely in the scattering process than photons of the surface layer of the sample, and the raman scattered photons return to the surface layer of the sample after multiple scattering and are collected. The position of the scattered light reaching the same depth in the sample after returning to the surface layer has the same spatial offset distance deltaS in the X direction on the surface layer of the sample from the incident point of the exciting light ₁ 。

When the distance from the conical top point of the conical mirror structure to the imaging surface is increased, the diameter of the formed light ring is also increased, and the width of the light ring is kept unchanged. The light beam has the characteristics of a Bessel light beam, and the light intensity distribution along the propagation direction of the light beam does not change. The size of the halo can be set by selecting a specific material and a conical mirror structure with a conical base angle and/or adjusting the distance from the vertex of the conical mirror structure to an imaging surface, and vice versa, after the spatial offset distance is converted for the subcutaneous depth required to be detected, the spatial offset distance can be used as the inner diameter for annular excitation, and the central area can collect signal light of the accumulated intensity generated by excitation.

A dichroic beam splitting element refers to an optical beam splitting element with dichroism, which is characterized by almost complete transmission of light of certain wavelengths and almost complete reflection of light of other wavelengths.

Based on the principle, the invention provides a subcutaneous detection device based on a conical mirror structure and a related detection system and method aiming at the particularity of the detection of the characteristic signal of the marker in the biological tissue, in particular to the acquisition of the deep biological characteristic signal of the subcutaneous tissue and aiming at solving all or part of the problems in the prior art.

The invention provides a subcutaneous detection device based on a conical mirror structure, which comprises an excitation light source assembly and a conical mirror structure which are sequentially arranged along a first light path, wherein the first light path is used for forming an annular excitation area on skin, the excitation light source assembly is used for emitting excitation light, and the excitation light emits signal light after being emitted or refracted by a subcutaneous biomarker; the device comprises a first imaging component, a second imaging component and a detector which are sequentially arranged along a second light path, wherein the second light path is used for collecting signal light; the excitation light source assembly comprises a laser and an optical collimating structure, light output by the laser is converted into parallel light beams through the optical collimating structure and enters the plane side of the conical mirror structure, and light emitted from the convex surface of the conical mirror structure is an annular light beam; the annular light beam forms an annular excitation area through the first light path; the first imaging component and the second imaging component form a conjugate optical structure; the central area of the annular excitation area and the receiving end of the detector are on a pair of conjugate planes.

The central area of the annular excitation area is a position with the same spatial offset distance to the excitation point, signal light reflects biological characteristic signals from the subcutaneous same depth level, the signals are accumulated to have high intensity, the excitation of the annular excitation area can realize more efficient single-point signal collection at the position of the central area, and meanwhile, the biological characteristic information of the subcutaneous specific depth meeting the actual requirement can be detected by selecting a specific conical mirror structure (material, shape and size) and/or adjusting the position of the conical mirror structure. More importantly, the side effects of local overheating, overhigh energy density and tissue burning risk caused by injection of point-like light spots can be effectively reduced by excitation of the annular excitation area, and the annular excitation area is particularly favorable for noninvasive detection of subcutaneous internal characteristic signals, and has safety and high reliability.

The conical mirror structure comprises a conical lens. The number of the conical lenses is single or multiple.

The cone angle of the axicon lens is in the range of 0.5-40 deg. Of these, 20 ° is preferred. The radius range of the annular excitation area is 1mm-9 mm. Preferably 2 mm.

The conical mirror structure further comprises a track fixed in a position parallel to the optical axis of the first optical path; the cone lens is connected with the rail in a sliding mode. And when the conical lens slides along the track, adjusting the distance from the conical vertex of the conical lens to an imaging surface.

The cone-shaped mirror structure is arranged in a through lens cone, and the size of through openings at two ends of the lens cone is matched with the light-transmitting aperture of the cone-shaped mirror structure; the rail is arranged on the side wall of the lens barrel.

The track is a sliding groove on the side wall of the lens barrel; a sliding block is arranged in the sliding groove and comprises a limiting part and a control part for controlling the movement of the limiting part, and the limiting part is fixedly connected with the edge of the conical lens; the control end of the control part is arranged outside the side wall of the lens barrel. The position of the conical lens is controlled by adopting a mechanical structure, the size of the annular excitation area is adjusted, and the conical lens is easy to manufacture, low in cost and convenient to operate.

The lens cone is made of aluminum materials subjected to oxidation blackening treatment.

The conical mirror structure is connected with the optical collimating structure through an optical fiber.

The conical mirror structure comprises an optical fiber and a conical lens arranged on the emergent end face of the optical fiber.

The optical alignment structure comprises an excitation optical fiber coupler which is connected with the laser through an optical fiber and converts the output light of the laser into parallel beams; the excitation fiber coupler is a plurality of GRIN lenses. The numerical aperture of the excitation fiber coupler is 0.22. The optical fiber is matched with the excitation optical fiber coupler to realize optical path connection, parallel light beams can be generated, the design of the optical path is more flexible, the bending of the optical path can be realized according to the design requirement of an actual structure, and the miniaturization or portability requirement of the design of the non-invasive detection equipment is better met.

The first light path and the second light path are partially overlapped, and a dichroic light splitting element is arranged at the intersection of the first light path and the second light path. The first or second light path can be diverted using a dichroic beam splitting element.

The dichroic light splitting element is arranged between the first imaging component and the second imaging component, is positioned between the conical mirror structure and the first imaging component, reflects emergent light of the conical mirror structure, enters the first imaging component, and is collimated by the first imaging component to form the annular excitation area; the signal light generated by the central area is transmitted from the dichroic light splitting element after being collected by the first imaging component. The first light path and the second light path can be partially overlapped, exciting light is separated from signal light, and the flexibility of the overall structural design of the subcutaneous detection device based on the conical mirror structure and the miniaturization requirement are facilitated.

And a band-pass filtering component is arranged in front of the second imaging component along the second optical path and is used for filtering stray light with the wavelength shorter than the wavelength of the signal light, and the central wavelength of the band-pass filtering component is matched with the wavelength of the laser.

The detector receives the signal light converged by the second imaging component through a collection optical fiber bundle, and the receiving end of the detector is the incident end face of the collection optical fiber bundle.

The first imaging component and/or the second imaging component comprises a focusing lens; the focal length of the focusing lens ranges from 5mm to 900 mm. Wherein the focal length of the focusing lens is preferably 20mm or 50 mm.

The first imaging component and/or the second imaging component comprise a lens group formed by at least 2 focusing lenses, and the focal length of the lens group can be adjusted, which is beneficial to the flexibility of practical application.

Including but not limited to 830nm semiconductor lasers, 785nm semiconductor lasers.

In another aspect, the present invention further provides a detection method, and the subcutaneous detection device based on the conical mirror structure of the present invention includes: s1, determining a spatial offset distance according to a subcutaneous specific depth level to be detected; s2, selecting the conical mirror structure, and arranging the conical mirror structure at a preset position on the first light path to form an annular excitation area with the size meeting the spatial offset distance; s3, arranging a first imaging component and a second imaging component on a pair of conjugate planes of a central area of the annular excitation area and a receiving end of the detector; and S4, collecting the signal light generated by excitation to the detector through the second light path for biological characteristic signal analysis.

In step S2, the position of the conical mirror structure is further adjusted along the first optical path to obtain annular excitation regions with different radii, so as to excite signal light from different subcutaneous depths.

The invention also provides a detection system for non-invasively detecting the blood biomarker information of the nail bed, which comprises an optical chamber and a support, wherein the optical chamber and the support form a finger or toe end placing chamber for accommodating a finger or toe; the support piece is movably connected with the optical bin; the optical chamber is used for integrating the parts of the subcutaneous detection device based on the conical mirror structure except the laser and the detector; one surface of the optical bin, which faces the supporting piece, is provided with an optical window, and the placing bin corresponds to the optical window; the excitation light provided by the optical bin is projected to the nail bed of the finger or toe to be detected through the optical window, the biological marker in the blood of the nail bed is detected, and the reflected or refracted signal light is collected, so that the information of the biological marker in the blood of the nail bed is obtained.

The optical bin provides exciting light and collects returned signal light, the exciting light is emitted through the optical window, and the returned signal light is collected. The supporting piece is used for supporting the finger or toe end of the living creature to be detected. The detection system is used for non-invasively detecting nail bed characteristic signals of the finger or toe end of a living being. When the biological marker in the nail bed blood is detected, the biological finger or toe end to be detected is placed in the biological finger or toe end placing bin, and the nail is correspondingly placed under the optical window, so that noninvasive detection can be performed, the operation is simple and efficient, the information of the biological marker in the nail bed blood is detected, and the disease or health condition of the nail bed is accurately reflected.

The optical chamber is connected with the supporting piece through a rotating piece, and a biological finger or toe end placing chamber is formed between the optical chamber and the supporting piece. The biological finger or toe end placing bin is used for placing the biological finger or toe end to be detected.

The rotating member is a hinge or a bearing. The optical bin can rotate anticlockwise through the hinge or the bearing, the optical bin preferably rotates 90 degrees anticlockwise, the light transmission condition of the optical window is convenient to check, and the optical window is convenient to replace when damaged.

The entrance of the biological finger or toe end placing bin is provided with a detachable rubber ring. The rubber ring is used for fixing the finger or toe end of the living creature to be detected and can be replaced according to the size of the finger or toe end of the living creature to be detected. The diameter of the rubber ring is preferably 10mm to 20 mm.

The optical bin and the support can also be slidably connected. A sliding groove is formed in the optical bin, and a sliding rail is arranged on the supporting piece; the supporting piece is fixed, the optical bin slides towards the direction far away from the supporting piece, and a biological finger or toe end placing bin is formed between the optical bin and the supporting piece according to the size of the biological finger or toe end to be detected.

Or the optical bin is provided with a slide rail, and the supporting piece is provided with a sliding groove; the optical bin is fixed, the support part slides towards the direction far away from the optical bin, and a biological finger or toe end placing bin is formed between the optical bin and the support part according to the size of the biological finger or toe end to be detected.

Or the optical bin and the supporting piece are in sliding connection through a sliding connecting piece. The optical cartridge and the support are simultaneously slidable, forming the biological finger or toe end placement cartridge between the optical cartridge and the support.

The optical window is a sheet structure. The thickness range of the optical window is 0.5mm-10mm, and preferably 1 mm; when the optical window is a circular sheet structure, the diameter of the optical window ranges from 0.5mm to 25mm, and preferably ranges from 5 mm.

The optical window is made of transparent resin or quartz glass. The optical window should be selected of a material that has a high transmittance and allows excitation light of a wavelength preferably 785nm or 830nm to pass through.

The invention also provides another detection system for detecting biomarker information under limb skin, which comprises an optical bin, an optical fiber transmission structure and a bandage, wherein the optical fiber transmission structure is used for optically connecting the optical bin and the bandage, and the bandage is used for accommodating limbs in a surrounding manner; the surface of the optical bin is provided with a light through hole, and the optical bin is used for integrating the parts of the subcutaneous detection device except the laser and the detector; the optical fiber transmission structure comprises an optical fiber bundle, a first optical fiber coupling system and a second optical fiber coupling system, wherein the optical fiber coupling systems are connected with two ends of the optical fiber bundle, the first optical fiber coupling system is used for leading the exciting light out of the optical bin, leading the exciting light into the binding band along the optical fiber bundle and the second optical fiber coupling system in sequence, reflecting or refracting the exciting light by a biomarker under limb skin to emit signal light, and leading the signal light into the optical bin along the second optical fiber coupling system, the optical fiber bundle and the first optical fiber coupling system in sequence; the optical fiber bundle is connected with the light through hole through the optical fiber coupling system. Transmitting the exciting light provided in the optical bin to the surface of the limb to be measured through the optical fiber transmission structure; and re-couples and re-transmits the signal light returned by the surface into the optical bin. The length of the optical fiber bundle is changed to meet different spatial positions of the optical bin and the limb to be detected, and the subcutaneous detection device is integrated through the optical bin, so that the subcutaneous detection device is convenient to use.

Compared with the prior art, the invention has the main beneficial effects that:

1. according to the subcutaneous detection device based on the conical mirror structure, annular excitation is formed based on the conical mirror structure, the collection of signal light can be enhanced by combining the first imaging part, the possible subcutaneous risk caused by point-shaped light spot excitation is avoided, the signal collection efficiency of subcutaneous noninvasive detection equipment is improved, and the safety is good; the reliability and the accuracy of the optical noninvasive detection are further optimized. The detection system is integrated with the subcutaneous detection device, and has the advantages of simple structure, low cost, high efficiency, flexible design and convenient arrangement.

2. The subcutaneous detection device provided by the invention has the advantages of simple steps and high efficiency, can acquire the biological characteristic information of the same subcutaneous depth level according to the requirement, and is favorable for further popularization and application of subcutaneous noninvasive detection equipment.

Drawings

Fig. 1 is a schematic diagram of spatial offset.

Fig. 2 is a schematic view of an annular excitation region and a central region according to a first embodiment of the invention.

Fig. 3 is a schematic view of a subcutaneous detection device based on a conical mirror structure according to a first embodiment of the present invention.

Fig. 4 is a schematic diagram of a detection method according to a first embodiment of the invention.

Fig. 5 is a schematic diagram of a conical mirror structure and related components according to a second embodiment of the invention.

Fig. 6 is a schematic diagram of a detection system in a third embodiment of the invention.

Fig. 7 is a schematic diagram of a detection system in a fourth embodiment of the invention.

FIG. 8 is a schematic diagram illustrating the rotation of an optical chamber according to a fourth embodiment of the present invention.

Fig. 9 is a schematic diagram of a detection system and a related structure in the fifth embodiment of the invention.

Detailed Description

The technical solutions in the specific embodiments of the present invention will be clearly and completely described below, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings. In the figures, parts of the same structure or function are denoted by the same reference numerals, and not all parts shown are denoted by the associated reference numerals in all figures for reasons of clarity of presentation.

Example one

Referring to fig. 1 in combination, as shown in fig. 3, the subcutaneous detection device based on the conical mirror structure of the present embodiment is integrated by an optical chamber I-1, and includes a laser 1, an excitation fiber coupler 102, and a conical mirror structure 3 arranged along a first optical path L1, wherein the laser 1 and the excitation fiber coupler 102 are used for emitting lightEmitting exciting light, wherein the exciting light emits signal light after being emitted or refracted by the subcutaneous biomarker; a first imaging section 2, a second imaging section 4, and a detector 5 disposed along a second optical path; the excitation fiber coupler 102 is directly connected with the laser 1 through an optical fiber 101, light output by the laser 1 is converted into parallel light beams to enter the plane side of the conical mirror structure 3, and light emitted from the convex surface of the conical mirror structure 3 is annular light beams; the annular light beam irradiates on the surface of the skin A through a first light path L1 to form an annular excitation area Q; the first imaging section 2 and the second imaging section 4 are a pair of focusing lenses of a conjugate structure. The focal length of the focusing lens ranges from 5mm to 900 mm. Wherein the focal length f of the first imaging member 2 ₁ Preferably 20mm and the focal length of the second imaging member is preferably 50 mm. In this embodiment, the excitation light source assembly includes a laser 1 and an optical collimating structure of the excitation fiber coupler 102, and for convenience of description, the first imaging component 2 and the second imaging component 4 are each a focusing lens, which is not limited to the example. The excitation light source assembly can comprise other optical elements, and the optical collimating structure can also adopt other optical elements such as a collimating lens; the number of the focusing lenses can be multiple, and the focal length can be adjusted and set according to actual needs, and is not limited. In a practical application of the embodiment, other components than the laser 1 and the detector 2 are integrated in the optical bin I-1. The signal light of the central region C generated by excitation of the annular excitation region Q enters the second light path L2 and is collected by the second imaging component 4, and the detector 5 receives the signal light converged by the second imaging component 4 through the collection optical fiber bundle 51. The incident end face of the collection optical fiber bundle 51 is on a pair of conjugate planes with the central region C, and the incident end face of the collection optical fiber bundle 51 is the incident face of the optical fiber coupler 52 in this embodiment. In the present embodiment, the central region C is located on the optical axis of the second light path L2. In this embodiment, the signal light to be collected is from the subcutaneous tissue as an example.

In this embodiment, the excitation fiber coupler 102 is a single or multiple GRIN lenses, and the numerical aperture of the excitation fiber coupler 102 is 0.22. Optical fiber is matched with the excitation optical fiber coupler 102 to realize optical path connection, so that not only can parallel light beams be generated, but also optical path bending can be realized according to the actual structural design requirement. In this embodiment, the output wavelength of the laser is preferably 785nm or 830 nm. An annular excitation area Q is generated based on the conical mirror structure 3, namely annular excitation is carried out, the central area C of the annular excitation area is a position with the same spatial offset distance offset from an excitation point (a point on the circumference of the annular excitation area Q), signal light at the position reflects biological characteristic signals from the same subcutaneous depth level, the signals are accumulated and have high intensity, and the excitation of the annular excitation area Q realizes efficient single-point signal collection at the position of the central area C, so that the side effects of local overheating, overhigh energy density and tissue burning risk caused by injection of punctiform light spots are effectively reduced.

In the optical path structure design in this embodiment, the first optical path L1 and the second optical path L2 are partially overlapped, and the dichroic beam splitter 6 is disposed at the intersection of the two. The dichroic beam splitting element 6 used in the present embodiment is a dichroic mirror, and can turn the first optical path or the second optical path to perform beam splitting. The dichroic beam splitter 6 may be a dichroic member such as a dichroic film, a dichroic plate, or a dichroic beam splitter, and is not limited thereto. The exit light of the cone mirror structure 3 is reflected while reaching between the first imaging member 2 and the second imaging member 4 using the dichroic beam splitting element 6 in the present embodiment, while transmitting the signal light collected by the first imaging member 2. Specifically, the dichroic mirror turns the first light path L1, converts the divergent annular light beam emitted from the conical mirror structure 3 into a collimated annular light beam, reflects the collimated annular light beam to the direction of the skin a, and transmits the signal light returning from the skin a, and in this embodiment, the reflection angle of the annular light beam at the dichroic mirror is preferably 45 °.

A bandpass filter T for filtering out stray light having a wavelength shorter than that of the signal light is provided along the second light path L2 before the second imaging element 4, and the center wavelength of the bandpass filter T is adapted to the wavelength of the laser. In the present embodiment, the band-pass filter member T is disposed on the second optical path L2 between the second imaging member 4 and the dichroic beam-splitting element 6. The collection quality can be improved, stray light is prevented from being collected as signal light, and the risk of signal distortion of the detector 5 is reduced.

In this embodiment, the conical mirror structure 3 comprises a conical lens. The cone angle of the axicon is in the range from 0.5 ° to 40 °, preferably 20 °, and the radius of the respective annular excitation region Q with the position of the axicon fixed is in the range from 1mm to 9mm, preferably 2 mm. The number of the axicons may be more than 1. The tapered mirror structure 3 may include, but is not limited to, an optical fiber and a tapered lens provided on an exit end surface of the optical fiber. In one practice of this embodiment, the tapered mirror structure 3 and the excitation fiber coupler 102 are connected by matching with an optical fiber 101.

As shown in fig. 4, the detection method in this embodiment includes: adopt subcutaneous detection device based on conical mirror structure of this embodiment, include: s1, determining a spatial offset distance according to a subcutaneous specific depth level to be detected; s2, selecting the conical mirror structure, and arranging the conical mirror structure at a preset position on a first light path to form an annular excitation area with the size meeting the spatial offset distance; s3, arranging a first imaging component and a second imaging component on a pair of conjugate planes of a central area of the annular excitation area and a receiving end of the detector; and S4, collecting the signal light generated by excitation to the detector through the second light path for biological characteristic signal analysis. In this embodiment, the size of the annular excitation region can be changed by replacing the cone lens with different cone angles, so as to change the spatial offset distance Δ S corresponding to the collected signal light in the central region ₁ And obtaining a biological characteristic signal of a specific subcutaneous depth.

Example two

The main difference between the second embodiment of the present invention and the first embodiment of the present invention is that, with reference to fig. 2 and fig. 3, as shown in fig. 5, the conical mirror structure 3 is disposed in the through lens barrel 7, the size of the through opening at both ends of the lens barrel 7 matches with the clear aperture of the conical mirror structure 3, and the aperture stop formed by the opening does not weaken the excitation light incident on the conical mirror structure 3. The track is provided with a chute G on the side wall 71 of the lens cone; a sliding block is arranged in the sliding groove G, the sliding block comprises a limiting part 81 and a control part 82 for controlling the movement of the limiting part, and the limiting part 81 is fixedly connected with the edge of the conical mirror structure 3 (the conical mirror structure 3 is a conical lens in this embodiment). The control end 82a of the control portion 82 is disposed outside the barrel side wall 71. The position of the conical mirror structure 3 is adjusted by adopting the position controlled by a mechanical structure, so that the conical mirror structure is easy to manufacture, low in cost and convenient to operate. The lens barrel 7 of the present embodiment is made of an aluminum material subjected to oxidation blackening treatment.

In the detection method of this embodiment, on the basis of the first embodiment, in the step S2, the position of the conical mirror structure 3 is further adjusted along the first optical path L1, so as to obtain annular excitation regions Q with different radii, and excite signal lights from different subcutaneous depths.

EXAMPLE III

The detection system in the embodiment is used for non-invasively detecting the biomarkers in the blood of the nail bed under the nail, and comprises an optical chamber I-1 and an optical window I-2 arranged on the optical chamber I-1, and is shown in figure 6.

The optical bin I-1 is used for integrating the subcutaneous detection device, providing exciting light and collecting returned signal light, projecting the exciting light to a nail bed to be detected through the optical window I-2, and collecting the returned signal light.

In this embodiment, the optical window I-2 is a circular sheet-like structure. The thickness of the optical window I-2 is 1mm, and the diameter of the optical window is 5 mm. In other embodiments, the optical window I-2 can also be configured as an oval, square, rectangle, or the like. The material of the optical window I-2 is transparent resin, and the selected material has high transmittance and can allow excitation light with the wavelength of 785nm or 830nm to transmit.

During detection, the fingernail of the finger to be detected is placed under the optical window I-2, the excitation light provided by the optical bin I-1 is projected to the nail bed to be detected through the optical window I-2, the biomarker in the blood of the nail bed is detected, and the returned signal light is collected, so that the information of the biomarker in the blood of the nail bed is obtained.

Example four

The embodiment integrates the subcutaneous detection device in any one of the first to the third embodiments, and provides a detection system for non-invasively detecting the biomarkers in the blood of the nail bed, which is used for non-invasively detecting the biomarkers in the blood of the nail bed under the nail, and the detection system comprises an optical bin I-1 and a support piece I-5, wherein the optical bin I-1 is movably connected with the support piece I-5, in particular, is connected through a rotating piece; a finger or toe end placement chamber I-4 is formed between the optical chamber I-1 and the support member I-5.

The optical bin I-1 is used for integrating the part of any subcutaneous detection device except the laser and the detector in the first embodiment to the third embodiment of the invention, is externally connected with the laser and the detector to provide exciting light when in use, and is used for collecting signal light reflected or refracted by skin; an optical window I-2 is further arranged on the optical bin I-1, and the optical window I-2 is arranged on one surface, facing the support piece I-5, of the optical bin I-1.

In this embodiment, the optical window I-2 is in a square sheet structure, but not limited to a square sheet structure, and the side length of the optical window I-2 is 10mm, and the thickness is 1 mm; the material of the optical window I-2 is quartz glass, and the selected material has high transmittance and can allow excitation light with the wavelength of 785nm or 830nm to transmit. The excitation light provided by the optical bin I-1 is projected to a nail bed to be detected through the optical window I-2, the biological marker in the blood of the nail bed is detected, and the returned signal light is collected, so that the information of the biological marker in the blood of the nail bed is obtained.

Referring to fig. 8, the optical chamber I-1 is connected with the supporting member I-5 through a rotating member I-3, the rotating member I-3 is a hinge, the optical chamber I-1 can rotate counterclockwise through the hinge, the counterclockwise rotation angle of the optical chamber I-1 is preferably 90 degrees, the light transmission condition of the optical window I-2 can be conveniently checked, and the optical window I-2 can be conveniently replaced when damaged.

A finger or toe end placing bin I-4 is formed between the optical bin I-1 and the supporting piece I-5, the finger or toe end placing bin I-4 corresponds to the optical window I-2 to contain a finger or toe, and the finger or toe end placing bin I-4 is used for placing a finger to be detected; the support piece I-5 is used for supporting the finger to be detected. When a finger to be detected is placed in the finger or toe end placing bin I-4, the fingernail of the finger to be detected is placed right below the optical window I-2.

The rubber ring I-6 is arranged at the entrance of the finger or toe end placing bin I-4, and the rubber ring I-6 can be detached and replaced and is used for fixing the finger to be detected and preventing detection errors caused by accidental sliding of the finger to be detected. The rubber ring I-6 can be made into different sizes so as to be suitable for fingers to be detected with different thicknesses. In this embodiment, the diameter of the rubber ring I-6 is preferably 15 mm.

When the biomarkers in the nail bed blood of the finger need to be detected, the finger to be detected is placed in the finger or toe end placing bin I-4, the nail of the finger to be detected is correspondingly placed under the optical window I-2, the excitation light provided by the optical bin I-1 is projected to the nail bed to be detected through the optical window I-2, the biomarkers in the nail bed blood are detected, and the returned signal light is collected, so that the information of the biomarkers in the nail bed blood is obtained.

Preferably, the optical chamber i-1 is used for integrating any subcutaneous detection device in the first to third embodiments of the present invention, including the laser and the detector.

EXAMPLE five

The present embodiment integrates any subcutaneous detection device in the first to third embodiments, and provides a detection system for detecting biomarker information under limb skin. Referring to fig. 9, the detection system comprises an optical bin i-1, an optical fiber transmission structure and a binding band ii-5, wherein the optical fiber transmission structure is used for optically connecting the optical bin i-1 and the binding band ii-5, and the binding band ii-5 is used for surrounding and accommodating a limb ii-7. The optical bin I-1 is used for integrating the parts of any subcutaneous detection device except the laser and the detector in the first embodiment to the third embodiment of the invention; when the device is used, the optical bin is externally connected with the excitation light source and the detector and is used for providing excitation light to be transmitted to the skin through the optical bin, and the optical bin is used for collecting signal light reflected or refracted by the skin and analyzing the signal light. And a detection window II-4 is arranged on the binding band. The optical fiber transmission structure comprises a first optical fiber coupling system II-2 and a second optical fiber coupling system II-3 which are connected with the light through hole, and an optical fiber bundle II-6 which is connected with the first optical fiber coupling system II-2 and the second optical fiber coupling system II-3. The optical fiber transmission structure transmits the exciting light provided in the optical bin I-1 to the skin surface of the limb to be detected; and the signal light returned from the skin surface is transmitted to the optical bin I-1 for analysis. The optical fiber transmission structure connects the optical bin I-1 with the detection window II-4. The detection windows II-4 are arranged on the outer side of the binding band II-5, the number of the detection windows II-4 is 4-15, and the detection windows II-4 are uniformly distributed on the binding band II-5; preferably, the number of collection windows II-4 is 12. Each detection window II-4 can be correspondingly connected with an optical bin I-1, when the biological markers in tissue fluid or blood below the skin of the limb II-7 are detected, the limb is placed on the inner side of a binding band II-5, the binding band II-5 is made of nylon materials and can be directly attached to the limb, excitation light in the optical bin I-1 is transmitted to the surface of the skin of the limb to be detected through an optical fiber transmission structure, and then signals returned by the detected skin are transmitted to the optical bin I-1 through the optical fiber transmission structure and are analyzed.

For clarity of description, the use of certain conventional and specific terms and phrases is intended to be illustrative and not restrictive, but rather to limit the scope of the invention to the particular letter and translation thereof. It is further noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. The present invention has been described in detail, and the structure and operation principle of the present invention are explained by applying specific embodiments, and the above description of the embodiments is only used to help understanding the method and core idea of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the principles of the invention, and it is intended to cover such changes and modifications as fall within the scope of the appended claims.

Claims

1. The utility model provides a subcutaneous detection device based on toper mirror structure which characterized in that: the skin-care device comprises an excitation light source assembly and a conical mirror structure, wherein the excitation light source assembly and the conical mirror structure are arranged along a first light path, the first light path is used for forming an annular excitation area on skin, the excitation light source assembly is used for emitting excitation light, and the excitation light emits signal light after being emitted or refracted by a subcutaneous biomarker;

the first imaging component, the second imaging component and the detector are arranged along a second optical path, and the second optical path is used for collecting signal light;

the excitation light source assembly comprises a laser and an optical collimating structure, light output by the laser is converted into parallel light beams through the optical collimating structure and enters the plane side of the conical mirror structure, and light emitted from the convex surface of the conical mirror structure is an annular light beam; the annular light beam forms an annular excitation area through the first light path;

the first imaging component and the second imaging component form a conjugate optical structure; the central area of the annular excitation area and the receiving end of the detector are on a pair of conjugate planes.

2. The conical mirror structure-based subcutaneous detection device according to claim 1, wherein: the conical mirror structure comprises a conical lens, and the first imaging component and/or the second imaging component comprises a focusing lens.

3. The conical mirror structure-based subcutaneous detection device according to claim 2, wherein: the conical mirror structure further comprises a track fixed in a position parallel to the optical axis of the first optical path; the cone lens is connected with the rail in a sliding mode.

4. A conical mirror structure-based subcutaneous detection device according to any one of claims 1-3, characterized in that: the first light path and the second light path are partially overlapped, and a dichroic light splitting element is arranged at the intersection of the first light path and the second light path.

5. The conical mirror structure-based subcutaneous detection device according to claim 4, wherein: the dichroic light splitting element is arranged between the first imaging component and the second imaging component and between the conical mirror structure and the first imaging component, reflects emergent light of the conical mirror structure, enters the first imaging component, and is collimated by the first imaging component to form the annular excitation area; the signal light generated by the central area is transmitted from the dichroic beam splitting element after being collected by the first imaging component.

6. A conical mirror structure based subcutaneous detection device according to any of claims 1-3, wherein: and a band-pass filtering component is arranged in front of the second imaging component along the second optical path and is used for filtering stray light with the wavelength shorter than the wavelength of the signal light, and the central wavelength of the band-pass filtering component is matched with the wavelength of the laser.

7. A method of probing, comprising: a subcutaneous probe device using the conical mirror-based structure of any of claims 1-6, comprising:

s1, determining a spatial offset distance according to a subcutaneous specific depth level to be detected;

s2, selecting the conical mirror structure, and arranging the conical mirror structure at a preset position on the first light path to form an annular excitation area with the size meeting the spatial offset distance;

s3, arranging a first imaging component and a second imaging component on a pair of conjugate planes of a central area of the annular excitation area and a receiving end of the detector;

and S4, collecting the signal light generated by excitation to the detector through the second light path for biological characteristic signal analysis.

8. A method of detection as claimed in claim 7, wherein: in step S2, the method further includes adjusting the position of the conical mirror structure along the first optical path to obtain annular excitation regions with different radii, and exciting signal light from different subcutaneous depths.

9. A detection system for non-invasively detecting blood biomarker information for a nail bed, comprising: comprises an optical chamber and a support member, the optical chamber and the support member forming a finger or toe end placement chamber for receiving a finger or toe; the optical chamber is used for integrating the subcutaneous detection device of any one of claims 1-6 except the laser and the detector; one surface of the optical bin, which faces the supporting piece, is provided with an optical window, and the placing bin corresponds to the optical window; the excitation light provided by the optical bin is projected to the nail bed of the finger or toe to be detected through the optical window, the biological marker in the blood of the nail bed is detected, and the reflected or refracted signal light is collected, so that the information of the biological marker in the blood of the nail bed is obtained.

10. A detection system for detecting biomarker information under the skin of a limb, characterized by: comprising an optical cartridge, an optical fiber transmission structure for optically connecting the optical cartridge and the strap, and a strap for encircling a receiving limb; the optical chamber is used for integrating the subcutaneous detection device of any one of claims 1-6 except the laser and the detector; the optical fiber transmission structure comprises an optical fiber bundle, a first optical fiber coupling system and a second optical fiber coupling system, wherein the first optical fiber coupling system and the second optical fiber coupling system are connected with two ends of the optical fiber bundle, the first optical fiber coupling system is used for leading exciting light out of the optical bin, the exciting light is led into the binding band along the optical fiber bundle and the second optical fiber coupling system in sequence, signal light is emitted after being reflected or refracted by biological markers under limb skin, and the signal light is led into the optical bin along the second optical fiber coupling system, the optical fiber bundle and the first optical fiber coupling system in sequence.