CN115112647B - Detection system based on pesticide residue food - Google Patents
Detection system based on pesticide residue food Download PDFInfo
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- CN115112647B CN115112647B CN202211024050.4A CN202211024050A CN115112647B CN 115112647 B CN115112647 B CN 115112647B CN 202211024050 A CN202211024050 A CN 202211024050A CN 115112647 B CN115112647 B CN 115112647B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The application relates to a detection system based on pesticide residue food. The method comprises the following steps: the sample extraction tank comprises a first inlet and a second inlet which are arranged at intervals, the first inlet is used for throwing sample fragments, and the second inlet is used for throwing buffer solution; the overflow groove is arranged in the extraction tank, the diameter of the overflow groove is gradually increased from the bottom of the overflow groove to the top of the overflow groove, and a plurality of overflow ports are arranged at intervals on the overflow groove along the axial direction of the sample extraction tank; the stirring shaft is arranged along the axial direction of the sample extraction tank, penetrates through the overflow groove and is provided with stirring blades; the accommodating tank is arranged at the bottom of the overflow tank; the rotary disk, the center of rotary disk is provided with the rotation axis, is provided with the detection chamber in the rotary disk, detects the intracavity along keeping away from first detection pond and the second detection pond that the rotation axis set up, and the top in first detection pond and second detection pond is linked together, and the holding tank is through the top and the first detection pond intercommunication that detect the chamber.
Description
Technical Field
The application relates to the field of agricultural safety, in particular to a detection system based on pesticide residue food.
Background
The development of agricultural industrialization makes the production of agricultural products depend on exogenous substances such as pesticides, antibiotics and hormones. The amount of agricultural chemicals in agricultural products in China is high, and unreasonable use of the substances can lead to over standard of the pesticide residues in the agricultural products, influence the edible safety of consumers, and cause the consumers to have diseases, develop abnormally and even directly cause poisoning and death in severe cases. The overproof pesticide residue also affects the trade of agricultural products, countries in the world pay high attention to the problem of pesticide residue, and increasingly strict limit standards are regulated for pesticide residue in various agricultural and sideline products, so that the export of agricultural products in China faces severe challenges. The current pesticide detection mode has low automation degree and influences the detection efficiency.
Disclosure of Invention
In view of the above, it is necessary to provide a detection system based on pesticide residue food.
A pesticide residue food-based detection system comprising:
the sample extraction tank comprises a first inlet and a second inlet which are arranged at intervals, the first inlet is used for throwing sample fragments, and the second inlet is used for throwing buffer solution;
the overflow groove is arranged in the extraction tank, the diameter of the overflow groove is gradually increased from the bottom of the overflow groove to the top of the overflow groove, and a plurality of overflow ports are arranged at intervals on the overflow groove along the axial direction of the sample extraction tank;
the stirring shaft is arranged along the axial direction of the sample extraction tank, penetrates through the overflow groove and is provided with stirring blades;
the accommodating groove is arranged at the bottom of the overflow groove;
the rotary disc, the center of rotary disc is provided with the rotation axis, be provided with in the rotary disc and detect the chamber, detect the intracavity along keeping away from first detection pond and the second detection pond that the rotation axis set up, first detection pond with the top in second detection pond is linked together, the holding tank passes through the top in detection chamber with first detection pond intercommunication.
In one embodiment, the device further comprises a centrifugal limiting plate, wherein the centrifugal limiting plate covers the tops of the first detection pool and the second detection pool;
a first partition plate dividing the first detection cell into a first portion and a second portion, the first portion being close to the rotation shaft;
the first spring is arranged below the first detection pool bottom plate, and the first isolation plate is clamped between the first spring and the centrifugal limiting plate.
In one embodiment, further comprising:
the second partition plate divides the second detection cell into a third part and a fourth part, and the third part is close to the rotating shaft;
the second spring set up in the below of second detection pond bottom plate, the second division board presss from both sides and establishes the second spring with between the centrifugal limiting plate.
In one embodiment, the detection chamber and the centrifugal limit plate are provided with corresponding first and second openings, respectively.
In one embodiment, when the rotating disc rotates at a first rotating speed, the centrifugal limit plate moves away from the center of the rotating shaft and moves to the top of the second detection cell to continue to limit the second partition plate;
the first spring pushes the first separation plate away from the bottom of the first detection cell, and the first portion is communicated with the second portion.
In one embodiment, when the rotating disc rotates at a second rotation speed, the centrifugal limiting plate continues to move away from the rotating shaft, the second partition plate is released, the second spring pushes the second partition plate away from the bottom of the second detection cell, the third portion is communicated with the fourth portion, and the second rotation speed is greater than the first rotation speed.
The detection system based on pesticide residue food that this application embodiment provided draws jar, overflow launder, (mixing) shaft, holding tank and holding tank including the sample. The sample extraction tank comprises a first inlet and a second inlet which are arranged at intervals, wherein the first inlet is used for throwing sample fragments, and the second inlet is used for throwing buffer solution. The buffer may be a phosphate buffer. The sample pieces may include pakchoi, lettuce, etc. The overflow groove is arranged in the extraction tank, the diameter of the overflow groove is gradually increased from the bottom of the overflow groove to the top of the overflow groove, and a plurality of overflow ports are arranged at intervals along the axial direction of the sample extraction tank. The sample pieces and the buffer solution may be mixed in the overflow tank. The stirring shaft is arranged along the axial direction of the sample extraction tank. The stirring shaft penetrates through the overflow groove, and stirring blades are arranged on the stirring shaft. The stirring shaft rotates to enable the sample fragments and the buffer solution to be fully mixed to form a mixed solution. When the stirring shaft rotates, the liquid level of the mixed liquid close to the edge of the overflow tank rises under the action of centrifugal force, and the liquid impurities on the surface of the mixed liquid are less and purer, so that the measurement accuracy is improved. Therefore, the mixed liquid can flow out along the overflow port, and the degree of stirring of the mixed liquid can be controlled by adjusting the rotating speed of the stirring rod, and the overflow groove from which the mixed liquid can flow out can also be controlled.
The first detection cell may include acetylcholinesterase. A thioacetyl choline may be included in the second detection cell as well as a chromogenic agent. After the mixed solution enters the first detection pool, the rotating disc can promote the mixed solution and the acetylcholinesterase to be mixed and reacted when rotating to obtain a reaction solution. After the reaction reaches a certain degree, the rotating speed of the rotating disc can be improved, the reaction solution can cross the opening of the first detection pool under the action of centrifugal force, and the reaction solution enters the second detection pool to react with the color developing agent to obtain yellow reaction liquid by connecting the first detection pool with the channel of the second detection pool. The whole process can realize two-step reaction by controlling the rotating speed of the rotating disc, thereby improving the working efficiency.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments or the conventional technologies of the present application, the drawings used in the description of the embodiments or the conventional technologies will be briefly introduced below, it is obvious that the drawings in the description below are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic view of a pesticide residue food-based detection system provided by an embodiment of the present application;
FIG. 2 is a schematic view of a pesticide residue food-based detection system provided in another embodiment of the present application;
fig. 3 is a schematic view of a pesticide residue food-based detection system according to another embodiment of the present application.
Description of the reference numerals
Stirring shaft 134
Stirring blade 136
Accommodating groove 140
Detection chamber 300
First opening 301
First detection cell 310
Second detection cell 320
Fourth portion 324
Centrifugal limit plate 340
First spring 352
A second spring 362.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is further described in detail below by way of embodiments and with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings). In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present application and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be considered as limiting the present application.
In this application, unless expressly stated or limited otherwise, a first feature is "on" or "under" a second feature such that the first and second features are in direct contact, or the first and second features are in indirect contact via an intermediary. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
Referring to fig. 1, the present application provides a detection system 10 for food based on pesticide residue. The inspection system includes a sample-extracting pot 100, an overflow bath 130, a stirring shaft 134, a housing tank 140, and a rotating disk 200. The sample extraction tank 100 comprises a first inlet 110 and a second inlet 120 which are arranged at intervals, wherein the first inlet 110 is used for throwing sample fragments, and the second inlet 120 is used for throwing buffer solution. The buffer may be a phosphate buffer. The sample pieces may include pakchoi, lettuce, and the like. The overflow groove 130 is disposed in the extraction pot, the diameter of the overflow groove 130 gradually increases from the bottom of the overflow groove 130 to the top of the overflow groove 130, and a plurality of overflow ports 132 are disposed at intervals in the overflow groove 130 along the axial direction of the sample extraction pot 100. The sample pieces and the buffer solution may be mixed in the overflow bath 130. The stirring shaft 134 is axially disposed along the sample extraction tank 100. The stirring shaft 134 penetrates through the overflow tank 130, and stirring blades 136 are arranged on the stirring shaft 134. The rotation of the stirring shaft 134 may cause the sample fragments and the buffer solution to be thoroughly mixed to form a mixed solution. In one embodiment, as the agitator shaft 134 rotates, the liquid level of the mixed liquor rises near the edge of the overflow tank 130 under the influence of centrifugal force. The mixed liquid can flow out along the overflow port 132. As can be seen from the illustration, the overflow outlets 132 can be multiple and located at different heights of the overflow trough 130. The user can select different overflow ports 132 to flow the mixed liquid according to the requirement, and other unnecessary overflow ports 132 can be blocked by a piston or the like. The mixed liquid flowing out of the overflow port 132 has a slower flow rate than that of the mixed liquid in the overflow groove 130 close to the stirring rod, thereby facilitating the flow control and simultaneously playing a certain role in filtering large impurities.
The holding tank 140 is disposed at the bottom of the overflow tank 130, so that the mixed liquid flowing out of the overflow tank 130 can enter the holding tank 140. The center of the rotary plate 200 is provided with a rotary shaft 210, and the rotary plate 200 is provided with a detection chamber 300 therein. The first detection cell 310 and the second detection cell 320 are disposed along the detection chamber 300 away from the rotation axis 210, the top of the first detection cell 310 is communicated with the top of the second detection cell 320, and the accommodating groove 140 is communicated with the first detection cell 310 through the top of the detection chamber 300. Therefore, the mixed solution can enter the first detection cell 310 in the accommodating cavity through the accommodating groove 140.
The system can adopt an enzyme inhibition rate method, takes the thio-acetylcholine as a substrate, the thio-acetylcholine is hydrolyzed into the thio-choline under the action of acetylcholinesterase, and the thio-choline can react with the color developing agent to generate 5-mercapto-2-nitrobenzoic acid, so that the reaction liquid is yellow. The change value of the absorbance along with the time is measured by a spectrophotometer at the wavelength of 410 nm, the inhibition rate is calculated, and whether high-dose organophosphorus and carbamate pesticides exist or not can be judged through the inhibition rate.
Acetylcholinesterase may be included in the first detection cell 310. A thioacetyl choline as a color-developing agent may be included in the second detection cell 320. After the mixed solution enters the first detection cell 310, the rotation of the rotating disc 200 can promote the mixed solution and the acetylcholinesterase to be mixed and reacted to obtain a reaction solution. After the reaction reaches a certain degree, the rotation speed of the rotating disk 200 may be increased, and the reaction solution may cross the opening of the first detection cell 310 under the action of centrifugal force, and enter the second detection cell 320 to react with the thioacetyl choline and the color developing agent through the channel connecting the first detection cell 310 and the second detection cell 320, so as to obtain a yellow reaction solution. The whole process can realize the mixing of experimental materials by controlling the rotating speed of the rotating disc 200, and the working efficiency is improved.
In one embodiment, a centrifugal retainer plate 340, a first spacer plate 350, and a first spring 352 are also included. The centrifugal limit plate 340 covers the tops of the first and second detection cells 310 and 320. The centrifugal limit plate 340 is arranged in the detection cavity 300. The first partition plate 350 divides the first detection cell 310 into a first portion 312 and a second portion 314. Acetylcholinesterase may be located in first portion 312. Mixed liquor may be injected into the second portion 314 before the rotating disk 200 rotates. A first spring 352 is provided below the bottom plate of the first detection cell 310, and the first partition plate 350 is interposed between the first spring 352 and the centrifugal limit plate 340. Under the action of the first spring 352 and the centrifugal stopper plate 340, the first partition plate 350 confines acetylcholinesterase within the first portion 312. Before detection, the acetylcholinesterase is limited in the first part 312, so that the space occupied by the acetylcholinesterase can be reduced, and the environment and the temperature of the acetylcholinesterase can be controlled independently.
In one embodiment, a second isolation plate 360, a second spring 362 is also included. The second partition plate 360 divides the second detection cell 320 into a third portion 322 and a fourth portion 324. A second spring 362 is disposed below the bottom plate of the second detection cell 320, and the second isolation plate 360 is sandwiched between the second spring 362 and the centrifugal limit plate 340. The third part can be thio-acetylcholine and color developing agent. The thioacetyl choline neutralizing reagent and the color developing reagent may be separately stored in the third portion 322 before the test is not performed, thereby preventing the thioacetyl choline neutralizing reagent and the color developing reagent from being exposed to an excessively large space and reducing interference of external environmental factors.
In one embodiment, the detection chamber 300 and the centrifugation limitation plate 340 are provided with corresponding first and second openings 301 and 342, respectively. When the rotating disc 200 is not rotated, the first opening 301 and the second opening 342 may be aligned, and the mixed solution may enter the first detection cell 310 through the first opening 301 and the second opening 342.
Referring to fig. 2, in one embodiment, when the rotating disc 200 rotates at the first rotating speed, the centrifugal stopper plate 340 moves away from the center of the rotating shaft 210 and moves to the top of the second detection cell 320 to continue to limit the second partition plate 360. After the centrifugal stopper plate 340 is separated from the first isolation plate 350, the first spring 352 pushes the first isolation plate 350 away from the bottom of the first detection cell 310, and the first portion 312 and the second portion 314 are communicated. At this point the first portion 312 of the mixture is mixed with the acetylcholinesterase enzyme in the second portion 314.
Referring to fig. 3, when the rotating disc 200 rotates at the second rotation speed, the centrifugal spacing plate 340 continues to move away from the rotating shaft 210, the second separating plate 360 is released, the second spring 362 pushes the second separating plate 360 away from the bottom of the second detection cell 320, and the third portion 322 is communicated with the fourth portion 324. The second rotational speed is greater than the first rotational speed. At this time, as the rotation speed is increased, the liquid in the first detection cell 310 overflows through the opening of the first detection cell 310 and enters the second detection cell 320, and at the same time, the fourth portion 324 is communicated with the third portion 322, so that the thioacetyl choline and the color developing agent can fully react with the liquid in the first detection cell 310 and change color in a large space.
It will be appreciated that the centrifugal force required to move the centrifugal retainer plate 340 away from the rotating shaft 210 can be controlled as desired, for example, at the first speed, the centrifugal retainer plate 340 can only release the first separating plate 350 and then cannot continue the centrifugal movement. While at the second rotational speed, the centrifugal stopper plate 340 can continue to move away from the rotating shaft 210. The centrifugal force of the centrifugal restriction plate 340 may be formed by a frictional force, and thus the displacement of the centrifugal restriction plate 340 can be controlled by controlling the maximum frictional force of the centrifugal restriction plate 340 with respect to the first and second detection cells 310 and 320.
And can be controlled by a common mechanical structure.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present patent. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (6)
1. A detection system based on pesticide residue food, characterized by comprising:
the sample extraction tank comprises a first inlet and a second inlet which are arranged at intervals, the first inlet is used for throwing sample fragments, and the second inlet is used for throwing buffer solution;
the overflow groove is arranged in the extraction tank, the diameter of the overflow groove is gradually increased from the bottom of the overflow groove to the top of the overflow groove, and a plurality of overflow ports are arranged at intervals along the axial direction of the sample extraction tank;
the stirring shaft is arranged along the axial direction of the sample extraction tank, penetrates through the overflow groove and is provided with stirring blades;
the accommodating groove is arranged at the bottom of the overflow groove;
the rotary disc, the center of rotary disc is provided with the rotation axis, be provided with in the rotary disc and detect the chamber, it sets gradually first detection pond and second detection pond along keeping away from to detect the intracavity the direction of rotation axis, first detection pond with the top in second detection pond is linked together, makes reaction solution can cross under the effect of centrifugal force the opening in first detection pond gets into the second detection pond, the holding tank passes through the top in detection chamber with first detection pond intercommunication.
2. The pesticide residue food-based detection system of claim 1, further comprising a centrifugal limit plate covering the top of the first detection tank and the second detection tank;
a first partition plate dividing the first detection cell into a first portion and a second portion, the first portion being close to the rotation shaft;
the first spring is arranged below the first detection pool bottom plate, and the first isolation plate is clamped between the first spring and the centrifugal limiting plate.
3. The pesticide residue food-based detection system of claim 2, further comprising:
the second partition plate divides the second detection cell into a third part and a fourth part, and the third part is close to the rotating shaft;
and the second spring is arranged below the second detection pool bottom plate, and the second partition plate is clamped between the second spring and the centrifugal limit plate.
4. The pesticide residue food based detection system of claim 3, wherein the detection chamber and the centrifugal limit plate are provided with corresponding first and second openings, respectively.
5. The pesticide-residue-based food detection system of claim 4, wherein when the rotating disk rotates at a first rotation speed, the centrifugal limit plate moves away from the center of the rotating shaft and moves to the top of the second detection tank to continue limiting the second partition plate;
the first spring pushes the first separation plate away from the bottom of the first detection cell, and the first portion is communicated with the second portion.
6. The pesticide residue food based detection system of claim 5, wherein when the rotating disc rotates at a second rotation speed, the centrifugal limiting plate continues to move away from the rotating shaft, the second separation plate is released, the second spring pushes the second separation plate away from the bottom of the second detection tank, the third portion is communicated with the fourth portion, and the second rotation speed is greater than the first rotation speed.
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| CN202211024050.4A CN115112647B (en) | 2022-08-25 | 2022-08-25 | Detection system based on pesticide residue food |
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| CN202211024050.4A CN115112647B (en) | 2022-08-25 | 2022-08-25 | Detection system based on pesticide residue food |
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| CN115112647B true CN115112647B (en) | 2022-12-09 |
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| CN216284913U (en) * | 2021-11-26 | 2022-04-12 | 锦州医科大学 | Food pesticide residue rapid detection device |
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